LOCUS       NM_183422               5961 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens TSC22 domain family member 1 (TSC22D1), transcript
            variant 1, mRNA.
ACCESSION   NM_183422
VERSION     NM_183422.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Kamimura,R., Uchida,D., Kanno,S.I., Shiraishi,R., Hyodo,T.,
            Sawatani,Y., Shimura,M., Hasegawa,T., Tsubura-Okubo,M., Yaguchi,E.,
            Komiyama,Y., Fukumoto,C., Izumi,S., Fujita,A., Wakui,T. and
            Kawamata,H.
  TITLE     Identification of Binding Proteins for TSC22D1 Family Proteins
            Using Mass Spectrometry
  JOURNAL   Int J Mol Sci 22 (20), 10913 (2021)
   PUBMED   34681573
  REMARK    GeneRIF: Identification of Binding Proteins for TSC22D1 Family
            Proteins Using Mass Spectrometry.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 5961)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 5961)
  AUTHORS   Homig-Holzel,C., van Doorn,R., Vogel,C., Germann,M., Cecchini,M.G.,
            Verdegaal,E. and Peeper,D.S.
  TITLE     Antagonistic TSC22D1 variants control BRAF(E600)-induced senescence
  JOURNAL   EMBO J 30 (9), 1753-1765 (2011)
   PUBMED   21448135
  REMARK    GeneRIF: Differential regulation of antagonistic TSC22D1 variants
            is required for the establishment of oncogene-induced cellular
            senescence.
REFERENCE   5  (bases 1 to 5961)
  AUTHORS   Lu,Y., Kitaura,J., Oki,T., Komeno,Y., Ozaki,K., Kiyono,M.,
            Kumagai,H., Nakajima,H., Nosaka,T., Aburatani,H. and Kitamura,T.
  TITLE     Identification of TSC-22 as a potential tumor suppressor that is
            upregulated by Flt3-D835V but not Flt3-ITD
  JOURNAL   Leukemia 21 (11), 2246-2257 (2007)
   PUBMED   17690703
  REMARK    GeneRIF: TSC-22 is a potential tumor suppressor that is upregulated
            by Flt3-D835V but not Flt3-ITD
REFERENCE   6  (bases 1 to 5961)
  AUTHORS   Sugawara,F., Yamada,Y., Watanabe,R., Ban,N., Miyawaki,K., Kuroe,A.,
            Hamasaki,A., Ikeda,H., Kurose,T., Usami,M., Ikeda,M. and Seino,Y.
  TITLE     The role of the TSC-22 (-396) A/G variant in the development of
            diabetic nephropathy
  JOURNAL   Diabetes Res Clin Pract 60 (3), 191-197 (2003)
   PUBMED   12757981
  REMARK    GeneRIF: findings suggest that the TSC-22 gene (-396) A allele is
            associated with an increasing risk of diabetic nephropathy
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 5961)
  AUTHORS   Kester,H.A., Blanchetot,C., den Hertog,J., van der Saag,P.T. and
            van der Burg,B.
  TITLE     Transforming growth factor-beta-stimulated clone-22 is a member of
            a family of leucine zipper proteins that can homo- and
            heterodimerize and has transcriptional repressor activity
  JOURNAL   J Biol Chem 274 (39), 27439-27447 (1999)
   PUBMED   10488076
REFERENCE   8  (bases 1 to 5961)
  AUTHORS   Ohta,S., Shimekake,Y. and Nagata,K.
  TITLE     Molecular cloning and characterization of a transcription factor
            for the C-type natriuretic peptide gene promoter
  JOURNAL   Eur J Biochem 242 (3), 460-466 (1996)
   PUBMED   9022669
REFERENCE   9  (bases 1 to 5961)
  AUTHORS   Jay,P., Ji,J.W., Marsollier,C., Taviaux,S., Berge-Lefranc,J.L. and
            Berta,P.
  TITLE     Cloning of the human homologue of the TGF beta-stimulated clone 22
            gene
  JOURNAL   Biochem Biophys Res Commun 222 (3), 821-826 (1996)
   PUBMED   8651929
REFERENCE   10 (bases 1 to 5961)
  AUTHORS   Shibanuma,M., Kuroki,T. and Nose,K.
  TITLE     Isolation of a gene encoding a putative leucine zipper structure
            that is induced by transforming growth factor beta 1 and other
            growth factors
  JOURNAL   J Biol Chem 267 (15), 10219-10224 (1992)
   PUBMED   1587811
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB082525.1, AL356107.21 and
            AL138960.16.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_183422.3.
            
            Summary: This gene encodes a member of the TSC22 domain family of
            leucine zipper transcription factors. The encoded protein is
            stimulated by transforming growth factor beta, and regulates the
            transcription of multiple genes including C-type natriuretic
            peptide. The encoded protein may play a critical role in tumor
            suppression through the induction of cancer cell apoptosis, and a
            single nucleotide polymorphism in the promoter of this gene has
            been associated with diabetic nephropathy. Alternatively spliced
            transcript variants encoding multiple isoforms have been observed
            for this gene. [provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1, also known as
            TSC22D1.1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB082525.1, BC136475.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2154665, SAMEA2157437
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000458659.3/ ENSP00000397435.2
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1251              AB082525.1         236-1486
            1252-1252           AL356107.21        64438-64438         c
            1253-3623           AB082525.1         1488-3858
            3624-5961           AL138960.16        140553-142890       c
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q14.11"
     gene            1..5961
                     /gene="TSC22D1"
                     /gene_synonym="HUCEP-2; Ptg-2; TGFB1I4; TSC22"
                     /note="TSC22 domain family member 1"
                     /db_xref="GeneID:8848"
                     /db_xref="HGNC:HGNC:16826"
                     /db_xref="MIM:607715"
     exon            1..3168
                     /gene="TSC22D1"
                     /gene_synonym="HUCEP-2; Ptg-2; TGFB1I4; TSC22"
                     /inference="alignment:Splign:2.1.0"
     CDS             257..3478
                     /gene="TSC22D1"
                     /gene_synonym="HUCEP-2; Ptg-2; TGFB1I4; TSC22"
                     /note="isoform 1 is encoded by transcript variant 1;
                     transforming growth factor beta-stimulated protein TSC-22;
                     cerebral protein 2; regulatory protein TSC-22;
                     TGFB-stimulated clone 22 homolog; transforming growth
                     factor beta-1-induced transcript 4 protein;
                     transcriptional regulator TSC-22; TGFbeta-stimulated clone
                     22"
                     /codon_start=1
                     /product="TSC22 domain family protein 1 isoform 1"
                     /protein_id="NP_904358.2"
                     /db_xref="CCDS:CCDS31966.1"
                     /db_xref="GeneID:8848"
                     /db_xref="HGNC:HGNC:16826"
                     /db_xref="MIM:607715"
                     /translation="MHQPPESTAAAAAAADISARKMAHPAMFPRRGSGSGSASALNAA
                     GTGVGSNATSSEDFPPPSLLQPPPPAASSTSGPQPPPPQSLNLLSQAQLQAQPLAPGG
                     TQMKKKSGFQITSVTPAQISASISSNNSIAEDTESYDDLDESHTEDLSSSEILDVSLS
                     RATDLGEPERSSSEETLNNFQEAETPGAVSPNQPHLPQPHLPHLPQQNVVINGNAHPH
                     HLHHHHQIHHGHHLQHGHHHPSHVAVASASITGGPPSSPVSRKLSTTGSSDSITPVAP
                     TSAVSSSGSPASVMTNMRAPSTTGGIGINSVTGTSTVNNVNITAVGSFNPNVTSSMLG
                     NVNISTSNIPSAAGVSVGPGVTSGVNVNILSGMGNGTISSSAAVSSVPNAAAGMTGGS
                     VSSQQQQPTVNTSRFRVVKLDSSSEPFKKGRWTCTEFYEKENAVPATEGVLINKVVET
                     VKQNPIEVTSERESTSGSSVSSSVSTLSHYTESVGSGEMGAPTVVVQQQQQQQQQQQQ
                     QPALQGVTLQQMDFGSTGPQSIPAVSIPQSISQSQISQVQLQSQELSYQQKQGLQPVP
                     LQATMSAATGIQPSPVNVVGVTSALGQQPSISSLAQPQLPYSQAAPPVQTPLPGAPPP
                     QQLQYGQQQPMVSTQMAPGHVKSVTQNPASEYVQQQPILQTAMSSGQPSSAGVGAGTT
                     VIPVAQPQGIQLPVQPTAVPAQPAGASVQPVGQAPAAVSAVPTGSQIANIGQQANIPT
                     AVQQPSTQVPPSVIQQGAPPSSQVVPPAQTGIIHQGVQTSAPSLPQQLVIASQSSLLT
                     VPPQPQGVEPVAQGIVSQQLPAVSSLPSASSISVTSQVSSTGPSGMPSAPTNLVPPQN
                     IAQTPATQNGNLVQSVSQPPLIATNTNLPLAQQIPLSSTQFSAQSLAQAIGSQIEDAR
                     RAAEPSLVGLPQTISGDSGGMSAVSDGSSSSLAASASLFPLKVLPLTTPLVDGEDESS
                     SGASVVAIDNKIEQAMDLVKSHLMYAVREEVEVLKEQIKELIEKNSQLEQENNLLKTL
                     ASPEQLAQFQAQLQTGSPPATTQPQGTTQPPAQPASQGSGPTA"
     exon            3169..3220
                     /gene="TSC22D1"
                     /gene_synonym="HUCEP-2; Ptg-2; TGFB1I4; TSC22"
                     /inference="alignment:Splign:2.1.0"
     exon            3221..5961
                     /gene="TSC22D1"
                     /gene_synonym="HUCEP-2; Ptg-2; TGFB1I4; TSC22"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4558..4563
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSC22D1"
                     /gene_synonym="HUCEP-2; Ptg-2; TGFB1I4; TSC22"
                     /note="hexamer: AATAAA"
     regulatory      4567..4572
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSC22D1"
                     /gene_synonym="HUCEP-2; Ptg-2; TGFB1I4; TSC22"
                     /note="hexamer: TATAAA"
     polyA_site      4582
                     /gene="TSC22D1"
                     /gene_synonym="HUCEP-2; Ptg-2; TGFB1I4; TSC22"
                     /note="major polyA site"
     polyA_site      4585
                     /gene="TSC22D1"
                     /gene_synonym="HUCEP-2; Ptg-2; TGFB1I4; TSC22"
     regulatory      5880..5885
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSC22D1"
                     /gene_synonym="HUCEP-2; Ptg-2; TGFB1I4; TSC22"
                     /note="hexamer: ATTAAA"
     polyA_site      5903
                     /gene="TSC22D1"
                     /gene_synonym="HUCEP-2; Ptg-2; TGFB1I4; TSC22"
     regulatory      5941..5946
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSC22D1"
                     /gene_synonym="HUCEP-2; Ptg-2; TGFB1I4; TSC22"
                     /note="hexamer: ATTAAA"
     polyA_site      5961
                     /gene="TSC22D1"
                     /gene_synonym="HUCEP-2; Ptg-2; TGFB1I4; TSC22"
ORIGIN      
        1 aaagtgaatg gcgaaggact gaagggatcc ccccttcggg tccccggccg ccctgttcac
       61 cctcgttcat cctcctttcc gaagctcgct ctcgaaggca ggagcgaccg gcgcctttgg
      121 ctgaggagga ggagaaggag gaatcgcgcc aggcggagcg tcaggtcccg ttttcctctc
      181 cggcgtctcc aatacaaaga ttacggtgca gaaggaaatt gcactcgtct cctccgcgcc
      241 cccggtaccc aacacaatgc accagccgcc tgagtccacc gccgcggccg ccgccgctgc
      301 agacattagc gctaggaaga tggcgcaccc ggcaatgttc cctcgaaggg gcagcggtag
      361 tggcagcgcc tctgctctca atgcagcagg taccggcgtc ggtagtaatg ccacatcttc
      421 cgaggatttt ccgcctccgt cgctgcttca gccgccgccc cctgcagcat cttctacgtc
      481 gggaccacag cctccgcctc cacaaagcct gaacctcctt tcgcaggctc agctgcaggc
      541 acagcctctt gcgccaggcg gaactcaaat gaaaaagaaa agtggcttcc agataactag
      601 cgttactcct gctcagatct ccgctagtat cagctctaac aacagtatag cagaggacac
      661 tgagagctat gatgatctgg atgaatctca cacggaagat ctctcttctt cggagatcct
      721 tgatgtgtca ctttccaggg ctactgactt aggggagccc gaacgcagct cctcagaaga
      781 gaccctaaat aacttccagg aagccgagac acctggggca gtctctccca accagcccca
      841 ccttcctcag cctcatttgc ctcaccttcc acaacagaat gttgtgatca atgggaatgc
      901 tcatccacac cacctccatc accaccatca gattcatcat gggcaccacc tccaacatgg
      961 tcaccaccat ccatctcatg ttgctgtggc cagtgcatcc attactggtg ggccaccctc
     1021 aagcccagta tctagaaaac tctctacaac tggaagctct gacagtatca caccagttgc
     1081 accaacttct gctgtatcat ccagtggttc acctgcatct gtaatgacta atatgcgtgc
     1141 tccaagtact acaggtggaa taggtataaa ttctgttact ggcactagta cagtaaataa
     1201 tgttaacatt actgctgtgg gtagttttaa tcctaatgtg acaagcagca tgcttggtaa
     1261 tgttaatata agtacaagca atattcctag tgctgctggt gtgagtgttg ggcctggagt
     1321 taccagtggt gttaatgtga atatcttgag tggcatgggc aatggtacta tttcttcctc
     1381 tgctgctgtt agcagtgttc ctaatgcagc tgcagggatg actgggggat cggtttcaag
     1441 tcagcagcaa caaccaacag ttaacacttc gaggttcaga gttgtgaagt tagattctag
     1501 ttctgagccc tttaaaaaag gtagatggac ttgcactgag ttctatgaaa aagaaaatgc
     1561 tgtacctgct acagaaggtg tgctgataaa taaagtggtg gagactgtaa agcaaaatcc
     1621 gatagaagtg acttctgaaa gggagagcac tagtgggagt tcagtgagca gtagtgtcag
     1681 cacactgagt cactatacag agagtgtggg aagtggagag atgggagccc ctactgtggt
     1741 ggtgcagcag cagcagcagc aacaacaaca acaacagcaa caaccagctc tccaaggtgt
     1801 gaccctccaa cagatggatt ttggtagcac tggtccacag agtattccag cagttagtat
     1861 accacagagt atttctcagt cacagatctc acaagtacaa ttacagtctc aagaactgag
     1921 ctatcagcaa aagcaaggtc ttcagccagt acctctgcaa gccactatga gtgctgcaac
     1981 tggtatccag ccatcgcctg taaatgtggt tggtgtaact tcagctttag gtcagcagcc
     2041 ttccatttcc agtttggctc aaccccagct accatattct caggcggctc ctccagtgca
     2101 aactcccctt ccaggggcac caccacccca acagttacag tatggacaac agcaaccaat
     2161 ggtttctaca cagatggccc caggccatgt caaatcagtg actcaaaatc ctgcttcaga
     2221 gtatgtacaa cagcagccaa ttcttcaaac agcaatgtcc tccggacagc ccagttctgc
     2281 aggagtagga gcaggaacaa cagtgattcc tgtggctcag ccacagggta tccagctgcc
     2341 agtgcagccc acagcagtcc cagcacaacc tgcaggggca tctgtccagc ctgttggcca
     2401 ggctccggca gcagtgtctg ctgtacctac tggcagtcag attgcaaata ttggtcagca
     2461 agcaaacata cctactgcag tgcagcagcc ctctacccag gttccacctt cagttattca
     2521 gcagggtgct cctccatctt cgcaagtggt tccacctgct caaactggga ttattcatca
     2581 gggagttcaa actagtgctc caagccttcc tcaacaattg gttattgcat cccaaagttc
     2641 cttgttaact gtgcctcccc agccacaagg agtagaacca gtagctcaag gaattgtttc
     2701 acagcagttg cctgcagtta gttctttgcc ctctgctagt agtatttctg ttacaagtca
     2761 ggttagttca actggtcctt ctggaatgcc ttctgcccca acaaacttgg ttccaccaca
     2821 aaatatagca caaacccctg ctacccaaaa tggtaatttg gttcaaagtg ttagtcaacc
     2881 tcccttgata gcaactaata caaatttgcc tttggcacaa cagataccac taagttctac
     2941 ccagttctcc gcacaatcat tagctcaggc aattggaagc caaattgaag atgccaggcg
     3001 tgcagcggag ccctccttag ttggcttacc tcagactatc agtggtgaca gtgggggaat
     3061 gtcagcagtt tcagatggga gtagcagcag cctagcagcc tctgcttctc ttttcccgtt
     3121 gaaggtgcta ccgctgacga cacccctggt ggatggcgag gatgagagct cctctggtgc
     3181 aagtgtggta gctattgaca acaaaatcga gcaagctatg gatctagtga aaagccattt
     3241 gatgtatgcg gtcagagaag aagtggaggt cctcaaagag caaatcaaag aactaataga
     3301 gaaaaattcc cagctggagc aggagaacaa tctgctgaag acactggcca gtcctgagca
     3361 gcttgcccag tttcaggccc agctgcagac tggctccccc cctgccacca cccagccaca
     3421 gggcaccaca cagccccccg cccagccagc atcgcagggc tcaggaccaa ccgcatagct
     3481 gcctatgccc ccgcagaact ggctgctgcg tgtgaactga acagacggag aagatgtgct
     3541 agggagaatc tgcctccaca gtcacccatt tcattgctcg ctgcgaaaga gacgtgagac
     3601 tgacatatgc cattatctct tttccagtat taaacactca tatgcttatg gcttggagaa
     3661 atttcttagt tgggtgaatt aaaggttaat ccgagaatta gcatggatat accgggacct
     3721 catgcagctt ggcagatatc tgagaaatgg tttaattcat gctcaggagc tgtgtgcctt
     3781 tccatccctt ccggctccct acccctcact tccaagggtt ctctctcctg cttgcgctta
     3841 gtgtcctaca tggggttgtg aagcgatgga gctcctcact ggactcgcct ctctcctctc
     3901 ctccccccag gaggaacttg aaaggagggt aaaaagacta aaatgagggg gaacagagtt
     3961 cactgtacaa atttgacaac tgtcaccaaa attcataaaa aacaatagta ctgtgcctct
     4021 ttcttctcaa acaatggatg acacaaaact atgagagtga caaaatggtg acaggtagct
     4081 gggacctagg ctatcttacc atgaaggttg ttttgcttat tgtatatttg tgtatgtagt
     4141 gtaactattt tgtacaatag aggactgtaa ctactattta ggttgtacag attgaaattt
     4201 agttgtttca ttggctgtct gaggaggtgt ggacttttat atatagatct acataaaaac
     4261 tgctacatga caaaaaccac acctaaagaa attttaagaa tttggcacag ttactcactt
     4321 tgtgtaatct gaaatctagc tgctgaatac gctgaagtaa atccttgttc actgaagtct
     4381 ttcaattgag ctggttgaat actttgaaaa atgctcagtt ctaactaatg aaatggattt
     4441 cccagtaggg gtttctgcat atcacctgta tagtagttat atgcatatgt ttctgtgcat
     4501 gttctctaca caattgtaag gtgtcactgt atttaactgt tgcacttgtc aactttcaat
     4561 aaagcatata aatgttgata aacaagtgtt tttcatatga ccctgttaac ataatggcag
     4621 tcatttccac aactgtttcc aggtaaagtt aacaatttga ctagtaaaat cctgaagggc
     4681 agtggaatga ttggaaaagt aggggcgtga tttgactgca tgaagtaaaa ggaaattgac
     4741 acattgagtt gttcagcagg taagagaaga gacagtggag gagtccagct caattctaac
     4801 tactgtgtga cttggacagg ttgcttcact cctggacgga tgtaggggtt tgaaagagat
     4861 gatctcttac attccccttt ggctttaaaa ttctaggagt ccttgaaaat cttaattttt
     4921 tactgaactg agaggaagaa ggagccttat tctgtgtata attggaataa gcagatctag
     4981 gatcaaaaga cggaagttgg agaagctgat ttccacttta agaaccctgt caccctgggt
     5041 tgggatgttt ctcctagtgt aggatgagtt tctagctcct gggataattc gttttgtcta
     5101 agcaaaagat gatttgaggt gggaccagat gagcaaggat gtccttgcag ttgattctgc
     5161 atgactataa aacaggccaa aattaaaacc acgaaggaat cctgaggcag attggcccct
     5221 ggcacctgag gagtgagcct gagggatccc actctgcagt aggagtaacg tgagcgagca
     5281 agtgaatggg gtgggtttac cggctctgta atctatcaac ctagggcctc agcagtggcc
     5341 ccctgcccag taggaattgg accaatccca agttctaagg cttgtcttaa gtccttgcag
     5401 aacaaggaca aactttctga atcattcatt tctctcatct agagctggtg ggagattgag
     5461 aggtgaatgg gacatccaag atccctaaaa agaattgttc gatagcgtgc atgtgttata
     5521 aagtggtgac acgggcatcc tgttgaaatg atggatggct cactgccata ggctgatagc
     5581 agttgtcata aagatatttt gggggaattt gaaaaggacg taaagaaaaa tctttcatat
     5641 tggcttgttg gttatataac ttcaaattta ataaaggaat acttacgtag taattacatt
     5701 tccttgaaaa aactatagtg aatagaaatc cctagccatt tcatttttta tgtttttaat
     5761 gaagatcttt aaaataccat aggtggtaat cgtggaaaat ttgaaaaatc tcatgtcagt
     5821 gtattaagat ggtggagaag tttttttctc cattatttaa tggaactttg ggtcttttta
     5881 ttaaaaatgt gagactcatg aaattttggc agctgaatat ttgtgaaata agaatgaatt
     5941 attaaaagat gtctttccat a
//
