LOCUS       NM_001114382            6346 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 5,
            mRNA.
ACCESSION   NM_001114382
VERSION     NM_001114382.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Fu,J., Liang,P., Zheng,Y., Xu,C., Xiong,F. and Yang,F.
  TITLE     A large deletion in TSC2 causes tuberous sclerosis complex by
            dysregulating PI3K/AKT/mTOR signaling pathway
  JOURNAL   Gene 909, 148312 (2024)
   PUBMED   38412945
  REMARK    GeneRIF: A large deletion in TSC2 causes tuberous sclerosis complex
            by dysregulating PI3K/AKT/mTOR signaling pathway.
            Erratum:[Gene. 2024 Aug 15;919:148481. doi:
            10.1016/j.gene.2024.148481.. PMID: 38685184]
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   Karalis,V., Wood,D., Teaney,N.A. and Sahin,M.
  TITLE     The role of TSC1 and TSC2 proteins in neuronal axons
  JOURNAL   Mol Psychiatry 29 (4), 1165-1178 (2024)
   PUBMED   38212374
  REMARK    GeneRIF: The role of TSC1 and TSC2 proteins in neuronal axons.
            Review article
REFERENCE   3  (bases 1 to 6346)
  AUTHORS   Li,M., Yan,X., Liu,H., Miao,W., Wu,W., Zhao,Y., Wang,C. and Liu,H.
  TITLE     Novel MSH2 and TSC2 variants in a Chinese family with Lynch
            syndrome and their synergistic impact in urothelial carcinoma
  JOURNAL   Transl Res 265, 26-35 (2024)
   PUBMED   37914149
  REMARK    GeneRIF: Novel MSH2 and TSC2 variants in a Chinese family with
            Lynch syndrome and their synergistic impact in urothelial
            carcinoma.
REFERENCE   4  (bases 1 to 6346)
  AUTHORS   Liao,W.T., Chiang,Y.J., Yang-Yen,H.F., Hsu,L.C., Chang,Z.F. and
            Yen,J.J.Y.
  TITLE     CBAP regulates the function of Akt-associated TSC protein complexes
            to modulate mTORC1 signaling
  JOURNAL   J Biol Chem 299 (12), 105455 (2023)
   PUBMED   37949232
  REMARK    GeneRIF: CBAP regulates the function of Akt-associated TSC protein
            complexes to modulate mTORC1 signaling.
            Erratum:[J Biol Chem. 2024 Feb;300(2):105686. doi:
            10.1016/j.jbc.2024.105686.. PMID: 38290174]
REFERENCE   5  (bases 1 to 6346)
  AUTHORS   Papageorgiou,G., Skouteris,N., Valavanis,C., Stanc,G.M., Souka,E.
            and Charalampakis,N.
  TITLE     Identification of a Novel TSC2 c.170G>A Missense Variant: A Case
            Report and Elaboration on the Yield of Targeted Options against
            Tuberous Sclerosis Complex Manifestations
  JOURNAL   Rev Recent Clin Trials 18 (4), 304-312 (2023)
   PUBMED   37877150
  REMARK    GeneRIF: Identification of a Novel TSC2 c.170G>A Missense Variant:
            A Case Report and Elaboration on the Yield of Targeted Options
            against Tuberous Sclerosis Complex Manifestations.
REFERENCE   6  (bases 1 to 6346)
  AUTHORS   Kumar,A., Wolpert,C., Kandt,R.S., Segal,J., Pufky,J., Roses,A.D.,
            Pericak-Vance,M.A. and Gilbert,J.R.
  TITLE     A de novo frame-shift mutation in the tuberin gene
  JOURNAL   Hum Mol Genet 4 (8), 1471-1472 (1995)
   PUBMED   7581393
REFERENCE   7  (bases 1 to 6346)
  AUTHORS   Xu,L., Sterner,C., Maheshwar,M.M., Wilson,P.J., Nellist,M.,
            Short,P.M., Haines,J.L., Sampson,J.R. and Ramesh,V.
  TITLE     Alternative splicing of the tuberous sclerosis 2 (TSC2) gene in
            human and mouse tissues
  JOURNAL   Genomics 27 (3), 475-480 (1995)
   PUBMED   7558029
REFERENCE   8  (bases 1 to 6346)
  AUTHORS   Northrup,H., Koenig,M.K., Pearson,D.A. and Au,K.S.
  TITLE     Tuberous Sclerosis Complex
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301399
REFERENCE   9  (bases 1 to 6346)
  AUTHORS   Kandt,R.S., Haines,J.L., Smith,M., Northrup,H., Gardner,R.J.,
            Short,M.P., Dumars,K., Roach,E.S., Steingold,S., Wall,S. et al.
  TITLE     Linkage of an important gene locus for tuberous sclerosis to a
            chromosome 16 marker for polycystic kidney disease
  JOURNAL   Nat Genet 2 (1), 37-41 (1992)
   PUBMED   1303246
REFERENCE   10 (bases 1 to 6346)
  AUTHORS   Webb,D.W. and Osborne,J.P.
  TITLE     Non-penetrance in tuberous sclerosis
  JOURNAL   J Med Genet 28 (6), 417-419 (1991)
   PUBMED   1870099
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC402106.1, BC150300.1,
            AK295672.1, CA427637.1 and AC093513.3.
            
            On Aug 13, 2020 this sequence version replaced NM_001114382.2.
            
            Summary: This gene is a tumor suppressor gene that encodes the
            growth inhibitory protein tuberin. Tuberin interacts with hamartin
            to form the TSC protein complex which functions in the control of
            cell growth. This TSC protein complex negatively regulates
            mammalian target of rapamycin complex 1 (mTORC1) signaling which is
            a major regulator of anabolic cell growth. Mutations in this gene
            have been associated with tuberous sclerosis and
            lymphangioleiomyomatosis. [provided by RefSeq, May 2022].
            
            Transcript Variant: This variant (5) lacks an alternate in-frame
            exon in the 3' coding region, compared to variant 1. It encodes
            isoform 5, which is shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC150300.1, SRR11853561.10811.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                DC402106.1         93-127
            36-738              BC150300.1         1-703
            739-758             AK295672.1         549-568
            759-2207            BC150300.1         724-2172
            2208-2220           AK295672.1         2018-2030
            2221-3076           BC150300.1         2186-3041
            3077-3153           AK295672.1         2758-2834
            3154-5030           BC150300.1         3119-4995
            5031-5057           AK295672.1         4712-4738
            5058-5567           BC150300.1         5023-5532
            5568-5575           CA427637.1         18-25               c
            5576-6346           AC093513.3         1610-2380           c
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..6346
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /note="TSC complex subunit 2"
                     /db_xref="GeneID:7249"
                     /db_xref="HGNC:HGNC:12363"
                     /db_xref="MIM:191092"
     exon            1..81
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            82..248
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..5465
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /note="isoform 5 is encoded by transcript variant 5;
                     tuberin; tuberous sclerosis 2 protein; protein phosphatase
                     1, regulatory subunit 160"
                     /codon_start=1
                     /product="tuberin isoform 5"
                     /protein_id="NP_001107854.1"
                     /db_xref="CCDS:CCDS45384.1"
                     /db_xref="GeneID:7249"
                     /db_xref="HGNC:HGNC:12363"
                     /db_xref="MIM:191092"
                     /translation="MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEI
                     LRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVL
                     ALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEEL
                     ADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVS
                     LQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNM
                     CHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVV
                     SYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVE
                     ELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALME
                     RFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLA
                     TQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLG
                     LLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRAD
                     SLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLG
                     SVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSM
                     LSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIH
                     RCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHL
                     YRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITK
                     GLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAA
                     EAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMM
                     ARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGEL
                     QSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPA
                     SQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTG
                     NTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAK
                     PPPLPRSNTDSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQE
                     EKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDP
                     GDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPR
                     GYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKP
                     ILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFL
                     TGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKH
                     RCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDM
                     EGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKR
                     LRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV
                     "
     exon            249..335
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            336..446
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            447..591
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            592..709
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            710..758
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            759..884
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            885..958
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            959..1085
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1229
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1230..1367
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1471
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..1553
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1554..1709
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1710..1826
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1827..1949
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1950..2056
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2057..2207
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2208..2330
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2331..2465
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2466..2655
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2656..2749
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2750..2852
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2853..2947
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2948..3076
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            3077..3241
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            3242..3394
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            3395..3507
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            3508..3720
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            3721..3924
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            3925..4046
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            4047..4534
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            4535..4610
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            4611..4703
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            4704..4890
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            4891..5030
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            5031..5109
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            5110..5201
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            5202..5300
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            5301..6346
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5522..5527
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /note="hexamer: AATAAA"
     polyA_site      5567
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /note="major polyA site"
     polyA_site      5598
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
     regulatory      6319..6324
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /note="hexamer: AATAAA"
     polyA_site      6346
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
ORIGIN      
        1 gcttccggcg gcgtcccggg gccagggggg tgcgcctttc tccgcgtcgg ggcggcccgg
       61 agcgcggtgg cgcggcgcgg gaggggtttt ctggtgcgtc ctggtccacc atggccaaac
      121 caacaagcaa agattcaggc ttgaaggaga agtttaagat tctgttggga ctgggaacac
      181 cgaggccaaa tcccaggtct gcagagggta aacagacgga gtttatcatc accgcggaaa
      241 tactgagaga actgagcatg gaatgtggcc tcaacaatcg catccggatg atagggcaga
      301 tttgtgaagt cgcaaaaacc aagaaatttg aagagcacgc agtggaagca ctctggaagg
      361 cggtcgcgga tctgttgcag ccggagcggc cgctggaggc ccggcacgcg gtgctggctc
      421 tgctgaaggc catcgtgcag gggcagggcg agcgtttggg ggtcctcaga gccctcttct
      481 ttaaggtcat caaggattac ccttccaacg aagaccttca cgaaaggctg gaggttttca
      541 aggccctcac agacaatggg agacacatca cctacttgga ggaagagctg gctgactttg
      601 tcctgcagtg gatggatgtt ggcttgtcct cggaattcct tctggtgctg gtgaacttgg
      661 tcaaattcaa tagctgttac ctcgacgagt acatcgcaag gatggttcag atgatctgtc
      721 tgctgtgcgt ccggaccgcg tcctctgtgg acatagaggt ctccctgcag gtgctggacg
      781 ccgtggtctg ctacaactgc ctgccggctg agagcctccc gctgttcatc gttaccctct
      841 gtcgcaccat caacgtcaag gagctctgcg agccttgctg gaagctgatg cggaacctcc
      901 ttggcaccca cctgggccac agcgccatct acaacatgtg ccacctcatg gaggacagag
      961 cctacatgga ggacgcgccc ctgctgagag gagccgtgtt ttttgtgggc atggctctct
     1021 ggggagccca ccggctctat tctctcagga actcgccgac atctgtgttg ccatcatttt
     1081 accaggccat ggcatgtccg aacgaggtgg tgtcctatga gatcgtcctg tccatcacca
     1141 ggctcatcaa gaagtatagg aaggagctcc aggtggtggc gtgggacatt ctgctgaaca
     1201 tcatcgaacg gctccttcag cagctccaga ccttggacag cccggagctc aggaccatcg
     1261 tccatgacct gttgaccacg gtggaggagc tgtgtgacca gaacgagttc cacgggtctc
     1321 aggagagata ctttgaactg gtggagagat gtgcggacca gaggcctgag tcctccctcc
     1381 tgaacctgat ctcctataga gcgcagtcca tccacccggc caaggacggc tggattcaga
     1441 acctgcaggc gctgatggag agattcttca ggagcgagtc ccgaggcgcc gtgcgcatca
     1501 aggtgctgga cgtgctgtcc tttgtgctgc tcatcaacag gcagttctat gaggaggagc
     1561 tgattaactc agtggtcatc tcgcagctct cccacatccc cgaggataaa gaccaccagg
     1621 tccgaaagct ggccacccag ttgctggtgg acctggcaga gggctgccac acacaccact
     1681 tcaacagcct gctggacatc atcgagaagg tgatggcccg ctccctctcc ccacccccgg
     1741 agctggaaga aagggatgtg gccgcatact cggcctcctt ggaggatgtg aagacagccg
     1801 tcctggggct tctggtcatc cttcagacca agctgtacac cctgcctgca agccacgcca
     1861 cgcgtgtgta tgagatgctg gtcagccaca ttcagctcca ctacaagcac agctacaccc
     1921 tgccaatcgc gagcagcatc cggctgcagg cctttgactt cctgttgctg ctgcgggccg
     1981 actcactgca ccgcctgggc ctgcccaaca aggatggagt cgtgcggttc agcccctact
     2041 gcgtctgcga ctacatggag ccagagagag gctctgagaa gaagaccagc ggcccccttt
     2101 ctcctcccac agggcctcct ggcccggcgc ctgcaggccc cgccgtgcgg ctggggtccg
     2161 tgccctactc cctgctcttc cgcgtcctgc tgcagtgctt gaagcaggag tctgactgga
     2221 aggtgctgaa gctggttctg ggcaggctgc ctgagtccct gcgctataaa gtgctcatct
     2281 ttacttcccc ttgcagtgtg gaccagctgt gctctgctct ctgctccatg ctttcaggcc
     2341 caaagacact ggagcggctc cgaggcgccc cagaaggctt ctccagaact gacttgcacc
     2401 tggccgtggt tccagtgctg acagcattaa tctcttacca taactacctg gacaaaacca
     2461 aacagcgcga gatggtctac tgcctggagc agggcctcat ccaccgctgt gccagccagt
     2521 gcgtcgtggc cttgtccatc tgcagcgtgg agatgcctga catcatcatc aaggcgctgc
     2581 ctgttctggt ggtgaagctc acgcacatct cagccacagc cagcatggcc gtcccactgc
     2641 tggagttcct gtccactctg gccaggctgc cgcacctcta caggaacttt gccgcggagc
     2701 agtatgccag tgtgttcgcc atctccctgc cgtacaccaa cccctccaag tttaatcagt
     2761 acatcgtgtg tctggcccat cacgtcatag ccatgtggtt catcaggtgc cgcctgccct
     2821 tccggaagga ttttgtccct ttcatcacta agggcctgcg gtccaatgtc ctcttgtctt
     2881 ttgatgacac ccccgagaag gacagcttca gggcccggag tactagtctc aacgagagac
     2941 ccaagagtct gaggatagcc agacccccca aacaaggctt gaataactct ccacccgtga
     3001 aagaattcaa ggagagctct gcagccgagg ccttccggtg ccgcagcatc agtgtgtctg
     3061 aacatgtggt ccgcagcagg atacagacgt ccctcaccag tgccagcttg gggtctgcag
     3121 atgagaactc cgtggcccag gctgacgata gcctgaaaaa cctccacctg gagctcacgg
     3181 aaacctgtct ggacatgatg gctcgatacg tcttctccaa cttcacggct gtcccgaaga
     3241 ggtctcctgt gggcgagttc ctcctagcgg gtggcaggac caaaacctgg ctggttggga
     3301 acaagcttgt cactgtgacg acaagcgtgg gaaccgggac ccggtcgtta ctaggcctgg
     3361 actcggggga gctgcagtcc ggcccggagt cgagctccag ccccggggtg catgtgagac
     3421 agaccaagga ggcgccggcc aagctggagt cccaggctgg gcagcaggtg tcccgtgggg
     3481 cccgggatcg ggtccgttcc atgtcggggg gccatggtct tcgagttggc gccctggacg
     3541 tgccggcctc ccagttcctg ggcagtgcca cttctccagg accacggact gcaccagccg
     3601 cgaaacctga gaaggcctca gctggcaccc gggttcctgt gcaggagaag acgaacctgg
     3661 cggcctatgt gcccctgctg acccagggct gggcggagat cctggtccgg aggcccacag
     3721 ggaacaccag ctggctgatg agcctggaga acccgctcag ccctttctcc tcggacatca
     3781 acaacatgcc cctgcaggag ctgtctaacg ccctcatggc ggctgagcgc ttcaaggagc
     3841 accgggacac agccctgtac aagtcactgt cggtgccggc agccagcacg gccaaacccc
     3901 ctcctctgcc tcgctccaac acagactccg ccgtggtcat ggaggaggga agtccgggcg
     3961 aggttcctgt gctggtggag cccccagggt tggaggacgt tgaggcagcg ctaggcatgg
     4021 acaggcgcac ggatgcctac agcaggtcgt cctcagtctc cagccaggag gagaagtcgc
     4081 tccacgcgga ggagctggtt ggcaggggca tccccatcga gcgagtcgtc tcctcggagg
     4141 gtggccggcc ctctgtggac ctctccttcc agccctcgca gcccctgagc aagtccagct
     4201 cctctcccga gctgcagact ctgcaggaca tcctcgggga ccctggggac aaggccgacg
     4261 tgggccggct gagccctgag gttaaggccc ggtcacagtc agggaccctg gacggggaaa
     4321 gtgctgcctg gtcggcctcg ggcgaagaca gtcggggcca gcccgagggt cccttgcctt
     4381 ccagctcccc ccgctcgccc agtggcctcc ggccccgagg ttacaccatc tccgactcgg
     4441 ccccatcacg caggggcaag agagtagaga gggacgcctt aaagagcaga gccacagcct
     4501 ccaatgcaga gaaagtgcca ggcatcaacc ccagtttcgt gttcctgcag ctctaccatt
     4561 cccccttctt tggcgacgag tcaaacaagc caatcctgct gcccaatgag tcacagtcct
     4621 ttgagcggtc ggtgcagctc ctcgaccaga tcccatcata cgacacccac aagatcgccg
     4681 tcctgtatgt tggagaaggc cagagcaaca gcgagctcgc catcctgtcc aatgagcatg
     4741 gctcctacag gtacacggag ttcctgacgg gcctgggccg gctcatcgag ctgaaggact
     4801 gccagccgga caaggtgtac ctgggaggcc tggacgtgtg tggtgaggac ggccagttca
     4861 cctactgctg gcacgatgac atcatgcaag ccgtcttcca catcgccacc ctgatgccca
     4921 ccaaggacgt ggacaagcac cgctgcgaca agaagcgcca cctgggcaac gactttgtgt
     4981 ccattgtcta caatgactcc ggtgaggact tcaagcttgg caccatcaag ggccagttca
     5041 actttgtcca cgtgatcgtc accccgctgg actacgagtg caacctggtg tccctgcagt
     5101 gcaggaaaga catggagggc cttgtggaca ccagcgtggc caagatcgtg tctgaccgca
     5161 acctgccctt cgtggcccgc cagatggccc tgcacgcaaa tatggcctca caggtgcatc
     5221 atagccgctc caaccccacc gatatctacc cctccaagtg gattgcccgg ctccgccaca
     5281 tcaagcggct ccgccagcgg atctgcgagg aagccgccta ctccaacccc agcctacctc
     5341 tggtgcaccc tccgtcccat agcaaagccc ctgcacagac tccagccgag cccacacctg
     5401 gctatgaggt gggccagcgg aagcgcctca tctcctcggt ggaggacttc accgagtttg
     5461 tgtgaggccg gggccctccc tcctgcactg gccttggacg gtattgcctg tcagtgaaat
     5521 aaataaagtc ctgaccccag tgcacagaca tagaggcaca gattgcagtc agacagctct
     5581 tttattgact ttgtctgctt ggtgcggggg ttgggggggt gtcgaggctc tagaagcggc
     5641 catgcccaca gaagtggtac acagaagcag gcacagccag ctccgagggc cttgaggctg
     5701 cctgggccat acagcacact cgcgcgtgcg cgcgcgcaca cacacacaca cacagtcacc
     5761 ttcctccacc ctgggagcca gcccccagga ggagtctttt cctctaacca ccctggggtc
     5821 ctctgacatg cctagtcctg ctacttgccc agacctgatg ccagcaggcc tgggcgctgc
     5881 tctcttgcta cctggcctgg ggcaagggag gatgacaagg cctctggggt gatgagagtg
     5941 cctggcagac agctgtgccc ccagcaccgg cccaaggcca agctcgcatc caagcagcag
     6001 ccgggctgcc ataacgccac cacacctacc aagcgcagca ggtgttgggg gaggccagct
     6061 ctgggcgcag gcccctcagc cctagtgaaa atagtgacat acaaaaatat acacatttta
     6121 acaccatata aattactgac acgagacaca cagtgagacg gtgcagggag tacggtagga
     6181 actggagagg taataactta ggggcagggt ggcggcggtg caggctaacc ctccctgaag
     6241 ccagcagcct tagcagtggg ggacatctgc ccagggggtg gggccgggca cagcccgctg
     6301 tacctgagga ctcggggaaa taaattagca tctcagaggc tagaaa
//
