LOCUS       NM_000548               6415 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 1,
            mRNA.
ACCESSION   NM_000548
VERSION     NM_000548.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Fu,J., Liang,P., Zheng,Y., Xu,C., Xiong,F. and Yang,F.
  TITLE     A large deletion in TSC2 causes tuberous sclerosis complex by
            dysregulating PI3K/AKT/mTOR signaling pathway
  JOURNAL   Gene 909, 148312 (2024)
   PUBMED   38412945
  REMARK    GeneRIF: A large deletion in TSC2 causes tuberous sclerosis complex
            by dysregulating PI3K/AKT/mTOR signaling pathway.
            Erratum:[Gene. 2024 Aug 15;919:148481. doi:
            10.1016/j.gene.2024.148481.. PMID: 38685184]
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Karalis,V., Wood,D., Teaney,N.A. and Sahin,M.
  TITLE     The role of TSC1 and TSC2 proteins in neuronal axons
  JOURNAL   Mol Psychiatry 29 (4), 1165-1178 (2024)
   PUBMED   38212374
  REMARK    GeneRIF: The role of TSC1 and TSC2 proteins in neuronal axons.
            Review article
REFERENCE   3  (bases 1 to 6415)
  AUTHORS   Li,M., Yan,X., Liu,H., Miao,W., Wu,W., Zhao,Y., Wang,C. and Liu,H.
  TITLE     Novel MSH2 and TSC2 variants in a Chinese family with Lynch
            syndrome and their synergistic impact in urothelial carcinoma
  JOURNAL   Transl Res 265, 26-35 (2024)
   PUBMED   37914149
  REMARK    GeneRIF: Novel MSH2 and TSC2 variants in a Chinese family with
            Lynch syndrome and their synergistic impact in urothelial
            carcinoma.
REFERENCE   4  (bases 1 to 6415)
  AUTHORS   Liao,W.T., Chiang,Y.J., Yang-Yen,H.F., Hsu,L.C., Chang,Z.F. and
            Yen,J.J.Y.
  TITLE     CBAP regulates the function of Akt-associated TSC protein complexes
            to modulate mTORC1 signaling
  JOURNAL   J Biol Chem 299 (12), 105455 (2023)
   PUBMED   37949232
  REMARK    GeneRIF: CBAP regulates the function of Akt-associated TSC protein
            complexes to modulate mTORC1 signaling.
            Erratum:[J Biol Chem. 2024 Feb;300(2):105686. doi:
            10.1016/j.jbc.2024.105686.. PMID: 38290174]
REFERENCE   5  (bases 1 to 6415)
  AUTHORS   Papageorgiou,G., Skouteris,N., Valavanis,C., Stanc,G.M., Souka,E.
            and Charalampakis,N.
  TITLE     Identification of a Novel TSC2 c.170G>A Missense Variant: A Case
            Report and Elaboration on the Yield of Targeted Options against
            Tuberous Sclerosis Complex Manifestations
  JOURNAL   Rev Recent Clin Trials 18 (4), 304-312 (2023)
   PUBMED   37877150
  REMARK    GeneRIF: Identification of a Novel TSC2 c.170G>A Missense Variant:
            A Case Report and Elaboration on the Yield of Targeted Options
            against Tuberous Sclerosis Complex Manifestations.
REFERENCE   6  (bases 1 to 6415)
  AUTHORS   Kumar,A., Wolpert,C., Kandt,R.S., Segal,J., Pufky,J., Roses,A.D.,
            Pericak-Vance,M.A. and Gilbert,J.R.
  TITLE     A de novo frame-shift mutation in the tuberin gene
  JOURNAL   Hum Mol Genet 4 (8), 1471-1472 (1995)
   PUBMED   7581393
REFERENCE   7  (bases 1 to 6415)
  AUTHORS   Xu,L., Sterner,C., Maheshwar,M.M., Wilson,P.J., Nellist,M.,
            Short,P.M., Haines,J.L., Sampson,J.R. and Ramesh,V.
  TITLE     Alternative splicing of the tuberous sclerosis 2 (TSC2) gene in
            human and mouse tissues
  JOURNAL   Genomics 27 (3), 475-480 (1995)
   PUBMED   7558029
REFERENCE   8  (bases 1 to 6415)
  AUTHORS   Northrup,H., Koenig,M.K., Pearson,D.A. and Au,K.S.
  TITLE     Tuberous Sclerosis Complex
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301399
REFERENCE   9  (bases 1 to 6415)
  AUTHORS   Kandt,R.S., Haines,J.L., Smith,M., Northrup,H., Gardner,R.J.,
            Short,M.P., Dumars,K., Roach,E.S., Steingold,S., Wall,S. et al.
  TITLE     Linkage of an important gene locus for tuberous sclerosis to a
            chromosome 16 marker for polycystic kidney disease
  JOURNAL   Nat Genet 2 (1), 37-41 (1992)
   PUBMED   1303246
REFERENCE   10 (bases 1 to 6415)
  AUTHORS   Webb,D.W. and Osborne,J.P.
  TITLE     Non-penetrance in tuberous sclerosis
  JOURNAL   J Med Genet 28 (6), 417-419 (1991)
   PUBMED   1870099
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC402106.1, AK299343.1,
            X75621.1, AK295672.1, CA427637.1 and AC093513.3.
            
            On Nov 23, 2018 this sequence version replaced NM_000548.4.
            
            Summary: This gene is a tumor suppressor gene that encodes the
            growth inhibitory protein tuberin. Tuberin interacts with hamartin
            to form the TSC protein complex which functions in the control of
            cell growth. This TSC protein complex negatively regulates
            mammalian target of rapamycin complex 1 (mTORC1) signaling which is
            a major regulator of anabolic cell growth. Mutations in this gene
            have been associated with tuberous sclerosis and
            lymphangioleiomyomatosis. [provided by RefSeq, May 2022].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: X75621.1, LC224284.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMEA1968189, SAMEA1968540 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000219476.9/ ENSP00000219476.3
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                DC402106.1         93-121
            30-92               AK299343.1         1-63
            93-1219             X75621.1           1-1127
            1220-1229           AK295672.1         1030-1039
            1230-2515           X75621.1           1138-2423
            2516-2655           AK295672.1         2326-2465
            2656-5635           X75621.1           2564-5543
            5636-5644           CA427637.1         18-26               c
            5645-6415           AC093513.3         1610-2380           c
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..6415
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /note="TSC complex subunit 2"
                     /db_xref="GeneID:7249"
                     /db_xref="HGNC:HGNC:12363"
                     /db_xref="MIM:191092"
     exon            1..81
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            82..248
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..5534
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     tuberin; tuberous sclerosis 2 protein; protein phosphatase
                     1, regulatory subunit 160"
                     /codon_start=1
                     /product="tuberin isoform 1"
                     /protein_id="NP_000539.2"
                     /db_xref="CCDS:CCDS10458.1"
                     /db_xref="GeneID:7249"
                     /db_xref="HGNC:HGNC:12363"
                     /db_xref="MIM:191092"
                     /translation="MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEI
                     LRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVL
                     ALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEEL
                     ADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVS
                     LQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNM
                     CHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVV
                     SYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVE
                     ELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALME
                     RFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLA
                     TQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLG
                     LLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRAD
                     SLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLG
                     SVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSM
                     LSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIH
                     RCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHL
                     YRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITK
                     GLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAA
                     EAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMM
                     ARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGEL
                     QSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPA
                     SQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTG
                     NTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAK
                     PPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVLVEPPGLEDV
                     EAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQP
                     SQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGED
                     SRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPG
                     INPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGE
                     GQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCW
                     HDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNF
                     VHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVH
                     HSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEP
                     TPGYEVGQRKRLISSVEDFTEFV"
     exon            249..335
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            336..446
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            447..591
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            592..709
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            710..758
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            759..884
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            885..958
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            959..1085
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1229
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1230..1367
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1471
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..1553
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1554..1709
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1710..1826
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1827..1949
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1950..2056
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2057..2207
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2208..2330
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2331..2465
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2466..2655
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2656..2749
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2750..2852
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2853..2947
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2948..3076
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            3077..3241
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            3242..3394
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            3395..3507
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            3508..3720
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            3721..3924
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            3925..3993
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            3994..4115
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            4116..4603
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            4604..4679
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            4680..4772
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            4773..4959
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            4960..5099
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            5100..5178
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            5179..5270
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            5271..5369
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     exon            5370..6415
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5591..5596
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /note="hexamer: AATAAA"
     polyA_site      5636
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /note="major polyA site"
     polyA_site      5667
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
     regulatory      6388..6393
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
                     /note="hexamer: AATAAA"
     polyA_site      6415
                     /gene="TSC2"
                     /gene_synonym="LAM; PPP1R160; TSC4"
ORIGIN      
        1 gcttccggcg gcgtcccggg gccagggggg tgcgcctttc tccgcgtcgg ggcggcccgg
       61 agcgcggtgg cgcggcgcgg gaggggtttt ctggtgcgtc ctggtccacc atggccaaac
      121 caacaagcaa agattcaggc ttgaaggaga agtttaagat tctgttggga ctgggaacac
      181 cgaggccaaa tcccaggtct gcagagggta aacagacgga gtttatcatc accgcggaaa
      241 tactgagaga actgagcatg gaatgtggcc tcaacaatcg catccggatg atagggcaga
      301 tttgtgaagt cgcaaaaacc aagaaatttg aagagcacgc agtggaagca ctctggaagg
      361 cggtcgcgga tctgttgcag ccggagcggc cgctggaggc ccggcacgcg gtgctggctc
      421 tgctgaaggc catcgtgcag gggcagggcg agcgtttggg ggtcctcaga gccctcttct
      481 ttaaggtcat caaggattac ccttccaacg aagaccttca cgaaaggctg gaggttttca
      541 aggccctcac agacaatggg agacacatca cctacttgga ggaagagctg gctgactttg
      601 tcctgcagtg gatggatgtt ggcttgtcct cggaattcct tctggtgctg gtgaacttgg
      661 tcaaattcaa tagctgttac ctcgacgagt acatcgcaag gatggttcag atgatctgtc
      721 tgctgtgcgt ccggaccgcg tcctctgtgg acatagaggt ctccctgcag gtgctggacg
      781 ccgtggtctg ctacaactgc ctgccggctg agagcctccc gctgttcatc gttaccctct
      841 gtcgcaccat caacgtcaag gagctctgcg agccttgctg gaagctgatg cggaacctcc
      901 ttggcaccca cctgggccac agcgccatct acaacatgtg ccacctcatg gaggacagag
      961 cctacatgga ggacgcgccc ctgctgagag gagccgtgtt ttttgtgggc atggctctct
     1021 ggggagccca ccggctctat tctctcagga actcgccgac atctgtgttg ccatcatttt
     1081 accaggccat ggcatgtccg aacgaggtgg tgtcctatga gatcgtcctg tccatcacca
     1141 ggctcatcaa gaagtatagg aaggagctcc aggtggtggc gtgggacatt ctgctgaaca
     1201 tcatcgaacg gctccttcag cagctccaga ccttggacag cccggagctc aggaccatcg
     1261 tccatgacct gttgaccacg gtggaggagc tgtgtgacca gaacgagttc cacgggtctc
     1321 aggagagata ctttgaactg gtggagagat gtgcggacca gaggcctgag tcctccctcc
     1381 tgaacctgat ctcctataga gcgcagtcca tccacccggc caaggacggc tggattcaga
     1441 acctgcaggc gctgatggag agattcttca ggagcgagtc ccgaggcgcc gtgcgcatca
     1501 aggtgctgga cgtgctgtcc tttgtgctgc tcatcaacag gcagttctat gaggaggagc
     1561 tgattaactc agtggtcatc tcgcagctct cccacatccc cgaggataaa gaccaccagg
     1621 tccgaaagct ggccacccag ttgctggtgg acctggcaga gggctgccac acacaccact
     1681 tcaacagcct gctggacatc atcgagaagg tgatggcccg ctccctctcc ccacccccgg
     1741 agctggaaga aagggatgtg gccgcatact cggcctcctt ggaggatgtg aagacagccg
     1801 tcctggggct tctggtcatc cttcagacca agctgtacac cctgcctgca agccacgcca
     1861 cgcgtgtgta tgagatgctg gtcagccaca ttcagctcca ctacaagcac agctacaccc
     1921 tgccaatcgc gagcagcatc cggctgcagg cctttgactt cctgttgctg ctgcgggccg
     1981 actcactgca ccgcctgggc ctgcccaaca aggatggagt cgtgcggttc agcccctact
     2041 gcgtctgcga ctacatggag ccagagagag gctctgagaa gaagaccagc ggcccccttt
     2101 ctcctcccac agggcctcct ggcccggcgc ctgcaggccc cgccgtgcgg ctggggtccg
     2161 tgccctactc cctgctcttc cgcgtcctgc tgcagtgctt gaagcaggag tctgactgga
     2221 aggtgctgaa gctggttctg ggcaggctgc ctgagtccct gcgctataaa gtgctcatct
     2281 ttacttcccc ttgcagtgtg gaccagctgt gctctgctct ctgctccatg ctttcaggcc
     2341 caaagacact ggagcggctc cgaggcgccc cagaaggctt ctccagaact gacttgcacc
     2401 tggccgtggt tccagtgctg acagcattaa tctcttacca taactacctg gacaaaacca
     2461 aacagcgcga gatggtctac tgcctggagc agggcctcat ccaccgctgt gccagccagt
     2521 gcgtcgtggc cttgtccatc tgcagcgtgg agatgcctga catcatcatc aaggcgctgc
     2581 ctgttctggt ggtgaagctc acgcacatct cagccacagc cagcatggcc gtcccactgc
     2641 tggagttcct gtccactctg gccaggctgc cgcacctcta caggaacttt gccgcggagc
     2701 agtatgccag tgtgttcgcc atctccctgc cgtacaccaa cccctccaag tttaatcagt
     2761 acatcgtgtg tctggcccat cacgtcatag ccatgtggtt catcaggtgc cgcctgccct
     2821 tccggaagga ttttgtccct ttcatcacta agggcctgcg gtccaatgtc ctcttgtctt
     2881 ttgatgacac ccccgagaag gacagcttca gggcccggag tactagtctc aacgagagac
     2941 ccaagagtct gaggatagcc agacccccca aacaaggctt gaataactct ccacccgtga
     3001 aagaattcaa ggagagctct gcagccgagg ccttccggtg ccgcagcatc agtgtgtctg
     3061 aacatgtggt ccgcagcagg atacagacgt ccctcaccag tgccagcttg gggtctgcag
     3121 atgagaactc cgtggcccag gctgacgata gcctgaaaaa cctccacctg gagctcacgg
     3181 aaacctgtct ggacatgatg gctcgatacg tcttctccaa cttcacggct gtcccgaaga
     3241 ggtctcctgt gggcgagttc ctcctagcgg gtggcaggac caaaacctgg ctggttggga
     3301 acaagcttgt cactgtgacg acaagcgtgg gaaccgggac ccggtcgtta ctaggcctgg
     3361 actcggggga gctgcagtcc ggcccggagt cgagctccag ccccggggtg catgtgagac
     3421 agaccaagga ggcgccggcc aagctggagt cccaggctgg gcagcaggtg tcccgtgggg
     3481 cccgggatcg ggtccgttcc atgtcggggg gccatggtct tcgagttggc gccctggacg
     3541 tgccggcctc ccagttcctg ggcagtgcca cttctccagg accacggact gcaccagccg
     3601 cgaaacctga gaaggcctca gctggcaccc gggttcctgt gcaggagaag acgaacctgg
     3661 cggcctatgt gcccctgctg acccagggct gggcggagat cctggtccgg aggcccacag
     3721 ggaacaccag ctggctgatg agcctggaga acccgctcag ccctttctcc tcggacatca
     3781 acaacatgcc cctgcaggag ctgtctaacg ccctcatggc ggctgagcgc ttcaaggagc
     3841 accgggacac agccctgtac aagtcactgt cggtgccggc agccagcacg gccaaacccc
     3901 ctcctctgcc tcgctccaac acagtggcct ctttctcctc cctgtaccag tccagctgcc
     3961 aaggacagct gcacaggagc gtttcctggg cagactccgc cgtggtcatg gaggagggaa
     4021 gtccgggcga ggttcctgtg ctggtggagc ccccagggtt ggaggacgtt gaggcagcgc
     4081 taggcatgga caggcgcacg gatgcctaca gcaggtcgtc ctcagtctcc agccaggagg
     4141 agaagtcgct ccacgcggag gagctggttg gcaggggcat ccccatcgag cgagtcgtct
     4201 cctcggaggg tggccggccc tctgtggacc tctccttcca gccctcgcag cccctgagca
     4261 agtccagctc ctctcccgag ctgcagactc tgcaggacat cctcggggac cctggggaca
     4321 aggccgacgt gggccggctg agccctgagg ttaaggcccg gtcacagtca gggaccctgg
     4381 acggggaaag tgctgcctgg tcggcctcgg gcgaagacag tcggggccag cccgagggtc
     4441 ccttgccttc cagctccccc cgctcgccca gtggcctccg gccccgaggt tacaccatct
     4501 ccgactcggc cccatcacgc aggggcaaga gagtagagag ggacgcctta aagagcagag
     4561 ccacagcctc caatgcagag aaagtgccag gcatcaaccc cagtttcgtg ttcctgcagc
     4621 tctaccattc ccccttcttt ggcgacgagt caaacaagcc aatcctgctg cccaatgagt
     4681 cacagtcctt tgagcggtcg gtgcagctcc tcgaccagat cccatcatac gacacccaca
     4741 agatcgccgt cctgtatgtt ggagaaggcc agagcaacag cgagctcgcc atcctgtcca
     4801 atgagcatgg ctcctacagg tacacggagt tcctgacggg cctgggccgg ctcatcgagc
     4861 tgaaggactg ccagccggac aaggtgtacc tgggaggcct ggacgtgtgt ggtgaggacg
     4921 gccagttcac ctactgctgg cacgatgaca tcatgcaagc cgtcttccac atcgccaccc
     4981 tgatgcccac caaggacgtg gacaagcacc gctgcgacaa gaagcgccac ctgggcaacg
     5041 actttgtgtc cattgtctac aatgactccg gtgaggactt caagcttggc accatcaagg
     5101 gccagttcaa ctttgtccac gtgatcgtca ccccgctgga ctacgagtgc aacctggtgt
     5161 ccctgcagtg caggaaagac atggagggcc ttgtggacac cagcgtggcc aagatcgtgt
     5221 ctgaccgcaa cctgcccttc gtggcccgcc agatggccct gcacgcaaat atggcctcac
     5281 aggtgcatca tagccgctcc aaccccaccg atatctaccc ctccaagtgg attgcccggc
     5341 tccgccacat caagcggctc cgccagcgga tctgcgagga agccgcctac tccaacccca
     5401 gcctacctct ggtgcaccct ccgtcccata gcaaagcccc tgcacagact ccagccgagc
     5461 ccacacctgg ctatgaggtg ggccagcgga agcgcctcat ctcctcggtg gaggacttca
     5521 ccgagtttgt gtgaggccgg ggccctccct cctgcactgg ccttggacgg tattgcctgt
     5581 cagtgaaata aataaagtcc tgaccccagt gcacagacat agaggcacag attgcagtca
     5641 gacagctctt ttattgactt tgtctgcttg gtgcgggggt tgggggggtg tcgaggctct
     5701 agaagcggcc atgcccacag aagtggtaca cagaagcagg cacagccagc tccgagggcc
     5761 ttgaggctgc ctgggccata cagcacactc gcgcgtgcgc gcgcgcacac acacacacac
     5821 acagtcacct tcctccaccc tgggagccag cccccaggag gagtcttttc ctctaaccac
     5881 cctggggtcc tctgacatgc ctagtcctgc tacttgccca gacctgatgc cagcaggcct
     5941 gggcgctgct ctcttgctac ctggcctggg gcaagggagg atgacaaggc ctctggggtg
     6001 atgagagtgc ctggcagaca gctgtgcccc cagcaccggc ccaaggccaa gctcgcatcc
     6061 aagcagcagc cgggctgcca taacgccacc acacctacca agcgcagcag gtgttggggg
     6121 aggccagctc tgggcgcagg cccctcagcc ctagtgaaaa tagtgacata caaaaatata
     6181 cacattttaa caccatataa attactgaca cgagacacac agtgagacgg tgcagggagt
     6241 acggtaggaa ctggagaggt aataacttag gggcagggtg gcggcggtgc aggctaaccc
     6301 tccctgaagc cagcagcctt agcagtgggg gacatctgcc cagggggtgg ggccgggcac
     6361 agcccgctgt acctgaggac tcggggaaat aaattagcat ctcagaggct agaaa
//
