LOCUS       NM_000368               8598 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens TSC complex subunit 1 (TSC1), transcript variant 1,
            mRNA.
ACCESSION   NM_000368
VERSION     NM_000368.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Wang,C., Zhai,J.X. and Chen,Y.J.
  TITLE     Identification of a novel TSC1 variant in a family with
            developmental and epileptic encephalopathies: A case report and
            literature review
  JOURNAL   Medicine (Baltimore) 103 (42), e40151 (2024)
   PUBMED   39432612
  REMARK    GeneRIF: Identification of a novel TSC1 variant in a family with
            developmental and epileptic encephalopathies: A case report and
            literature review.
            Review article
REFERENCE   2  (bases 1 to 8598)
  AUTHORS   Chen,Y., Li,S. and He,H.
  TITLE     miR-27a-3p promotes inflammatory response in infectious
            endophthalmitis via targeting TSC1
  JOURNAL   Sci Rep 14 (1), 19353 (2024)
   PUBMED   39169069
  REMARK    GeneRIF: miR-27a-3p promotes inflammatory response in infectious
            endophthalmitis via targeting TSC1.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8598)
  AUTHORS   Liu,X., Li,W., Yi,L., Wang,J., Liu,W., Cheng,H. and Ren,S.
  TITLE     CDK4/6 inhibitors dephosphorylate RNF26 to stabilize TSC1 and
            increase the sensitivity of ccRCC to mTOR inhibitors
  JOURNAL   Br J Cancer 131 (3), 444-456 (2024)
   PUBMED   38890443
REFERENCE   4  (bases 1 to 8598)
  AUTHORS   Huang,J. and Manning,B.D.
  TITLE     The TSC1-TSC2 complex: a molecular switchboard controlling cell
            growth
  JOURNAL   Biochem J 412 (2), 179-190 (2008)
   PUBMED   18466115
  REMARK    Review article
REFERENCE   5  (bases 1 to 8598)
  AUTHORS   Huang,J., Dibble,C.C., Matsuzaki,M. and Manning,B.D.
  TITLE     The TSC1-TSC2 complex is required for proper activation of mTOR
            complex 2
  JOURNAL   Mol Cell Biol 28 (12), 4104-4115 (2008)
   PUBMED   18411301
  REMARK    GeneRIF: the TSC1-TSC2 complex inhibits mTORC1 and activates
            mTORC2, which through different mechanisms promotes Akt activation
REFERENCE   6  (bases 1 to 8598)
  AUTHORS   Plank,T.L., Yeung,R.S. and Henske,E.P.
  TITLE     Hamartin, the product of the tuberous sclerosis 1 (TSC1) gene,
            interacts with tuberin and appears to be localized to cytoplasmic
            vesicles
  JOURNAL   Cancer Res 58 (21), 4766-4770 (1998)
   PUBMED   9809973
REFERENCE   7  (bases 1 to 8598)
  AUTHORS   van Slegtenhorst,M., Nellist,M., Nagelkerken,B., Cheadle,J.,
            Snell,R., van den Ouweland,A., Reuser,A., Sampson,J., Halley,D. and
            van der Sluijs,P.
  TITLE     Interaction between hamartin and tuberin, the TSC1 and TSC2 gene
            products
  JOURNAL   Hum Mol Genet 7 (6), 1053-1057 (1998)
   PUBMED   9580671
REFERENCE   8  (bases 1 to 8598)
  AUTHORS   Jones,A.C., Daniells,C.E., Snell,R.G., Tachataki,M.,
            Idziaszczyk,S.A., Krawczak,M., Sampson,J.R. and Cheadle,J.P.
  TITLE     Molecular genetic and phenotypic analysis reveals differences
            between TSC1 and TSC2 associated familial and sporadic tuberous
            sclerosis
  JOURNAL   Hum Mol Genet 6 (12), 2155-2161 (1997)
   PUBMED   9328481
REFERENCE   9  (bases 1 to 8598)
  AUTHORS   van Slegtenhorst,M., de Hoogt,R., Hermans,C., Nellist,M.,
            Janssen,B., Verhoef,S., Lindhout,D., van den Ouweland,A.,
            Halley,D., Young,J., Burley,M., Jeremiah,S., Woodward,K.,
            Nahmias,J., Fox,M., Ekong,R., Osborne,J., Wolfe,J., Povey,S.,
            Snell,R.G., Cheadle,J.P., Jones,A.C., Tachataki,M., Ravine,D.,
            Sampson,J.R., Reeve,M.P., Richardson,P., Wilmer,F., Munro,C.,
            Hawkins,T.L., Sepp,T., Ali,J.B., Ward,S., Green,A.J., Yates,J.R.,
            Kwiatkowska,J., Henske,E.P., Short,M.P., Haines,J.H., Jozwiak,S.
            and Kwiatkowski,D.J.
  TITLE     Identification of the tuberous sclerosis gene TSC1 on chromosome
            9q34
  JOURNAL   Science 277 (5327), 805-808 (1997)
   PUBMED   9242607
REFERENCE   10 (bases 1 to 8598)
  AUTHORS   Northrup,H., Koenig,M.K., Pearson,D.A. and Au,K.S.
  TITLE     Tuberous Sclerosis Complex
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301399
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC070032.1, AF013168.1,
            D87683.1 and N63914.1.
            
            On Aug 31, 2019 this sequence version replaced NM_000368.4.
            
            Summary: This gene is a tumor suppressor gene that encodes the
            growth inhibitory protein hamartin. The encoded protein interacts
            with and stabilizes the GTPase activating protein tuberin. This
            hamartin-tuberin complex negatively regulates mammalian target of
            rapamycin complex 1 (mTORC1) signaling which is a major regulator
            of anabolic cell growth. This protein also functions as a
            co-chaperone for Hsp90 that inhibits its ATPase activity. This
            protein functions as a facilitator of Hsp90-mediated folding of
            kinase and non-kinase clients, including TSC2 and thereby
            preventing their ubiquitination and proteasomal degradation.
            Mutations in this gene have been associated with tuberous sclerosis
            and lymphangioleiomyomatosis. [provided by RefSeq, May 2022].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.2628004.1, AF013168.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000298552.9/ ENSP00000298552.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1246              BC070032.1         19-1264
            1247-4000           AF013168.1         1251-4004
            4001-5252           D87683.1           2389-3640
            5253-6364           AF013168.1         5256-6367
            6365-8597           D87683.1           4752-6984
            8598-8598           N63914.1           12-12               c
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.13"
     gene            1..8598
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /note="TSC complex subunit 1"
                     /db_xref="GeneID:7248"
                     /db_xref="HGNC:HGNC:12362"
                     /db_xref="MIM:605284"
     exon            1..74
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            75..137
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            138..323
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     CDS             218..3712
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /note="isoform 1 is encoded by transcript variant 1;
                     hamartin; tuberous sclerosis 1 protein"
                     /codon_start=1
                     /product="hamartin isoform 1"
                     /protein_id="NP_000359.1"
                     /db_xref="CCDS:CCDS6956.1"
                     /db_xref="GeneID:7248"
                     /db_xref="HGNC:HGNC:12362"
                     /db_xref="MIM:605284"
                     /translation="MAQQANVGELLAMLDSPMLGVRDDVTAVFKENLNSDRGPMLVNT
                     LVDYYLETSSQPALHILTTLQEPHDKHLLDRINEYVGKAATRLSILSLLGHVIRLQPS
                     WKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITMLPMIPQSGKQHLLDFFDIFGR
                     LSSWCLKKPGHVAEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEE
                     VVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYS
                     VSHQISARFPHRSADVTTSPYADTQNSYGCATSTPYSTSRLMLLNMPGQLPQTLSSPS
                     TRLITEPPQATLWSPSMVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPA
                     TSPPPAPLCHSDDYVHISLPQATVTPPRKEERMDSARPCLHRQHHLLNDRGSEEPPGS
                     KGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISRELSEITTAEAEPVVPRGGFDSPFY
                     RDSLPGSQRKTHSAASSSQGASVNPEPLHSSLDKLGPDTPKQAFTPIDLPCGSADESP
                     AGDRECQTSLETSIFTPSPCKIPPPTRVGFGSGQPPPYDHLFEVALPKTAHHFVIRKT
                     EELLKKAKGNTEEDGVPSTSPMEVLDRLIQQGADAHSKELNKLPLPSKSVDWTHFGGS
                     PPSDEIRTLRDQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKAAALEEHNAAMKDQL
                     KLQEKDIQMWKVSLQKEQARYNQLQEQRDTMVTKLHSQIRQLQHDREEFYNQSQELQT
                     KLEDCRNMIAELRIELKKANNKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLG
                     EVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQTQRLDTSQKRILELES
                     HLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKD
                     GLLKKLEEEKAEAAEAAEERLDCCNDGCSDSMVGHNEEASGHNGETKTPRPSSARGSS
                     GSRGGGGSSSSSSELSTPEKPPHQRAGPFSSRWETTMGEASASIPTTVGSLPSSKSFL
                     GMKARELFRNKSESQCDEDGMTSSLSESLKTELGKDLGVEAKIPLNLDGPHPSPPTPD
                     SVGQLHIMDYNETHHEHS"
     exon            324..427
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            428..580
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            581..725
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            726..880
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            881..954
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            955..1130
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1131..1246
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..1358
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1359..1480
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1481..1550
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1551..1655
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..2214
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2215..2258
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2259..2425
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2426..2608
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2609..2719
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2720..2842
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2843..3030
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            3031..3192
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            3193..8598
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8574..8579
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /note="hexamer: AATAAA"
     polyA_site      8598
                     /gene="TSC1"
                     /gene_synonym="LAM; TSC"
                     /note="major polyA site"
ORIGIN      
        1 gtacgtccaa gatggcggcg ccctgtaggc tggagggact gtgaggtaaa cagctgaggg
       61 ggaggagacg gtggtgacca tgaaagacac caggttgaca gcactggaaa ctgaagtacc
      121 agttgtcgct agaacagttt ggtagtggcc ccaatgaaga accttcagaa cctgtagcac
      181 acgtcctgga gccagcacag cgccttcgag cgagagaatg gcccaacaag caaatgtcgg
      241 ggagcttctt gccatgctgg actcccccat gctgggtgtg cgggacgacg tgacagctgt
      301 ctttaaagag aacctcaatt ctgaccgtgg ccctatgctt gtaaacacct tggtggatta
      361 ttacctggaa accagctctc agccggcatt gcacatcctg accaccttgc aagagccaca
      421 tgacaagcac ctcttggaca ggattaacga atatgtgggc aaagccgcca ctcgtttatc
      481 catcctctcg ttactgggtc atgtcataag actgcagcca tcttggaagc ataagctctc
      541 tcaagcacct cttttgcctt ctttactaaa atgtctcaag atggacactg acgtcgttgt
      601 cctcacaaca ggcgtcttgg tgttgataac catgctacca atgattccac agtctgggaa
      661 acagcatctt cttgatttct ttgacatttt tggccgtctg tcatcatggt gcctgaagaa
      721 accaggccac gtggcggaag tctatctcgt ccatctccat gccagtgtgt acgcactctt
      781 tcatcgcctt tatggaatgt acccttgcaa cttcgtctcc tttttgcgtt ctcattacag
      841 tatgaaagaa aacctggaga cttttgaaga agtggtcaag ccaatgatgg agcatgtgcg
      901 aattcatccg gaattagtga ctggatccaa ggaccatgaa ctggaccctc gaaggtggaa
      961 gagattagaa actcatgatg ttgtgatcga gtgtgccaaa atctctctgg atcccacaga
     1021 agcctcatat gaagatggct attctgtgtc tcaccaaatc tcagcccgct ttcctcatcg
     1081 ttcagccgat gtcaccacca gcccttatgc tgacacacag aatagctatg ggtgtgctac
     1141 ttctacccct tactccacgt ctcggctgat gttgttaaat atgccagggc agctacctca
     1201 gactctgagt tccccatcga cacggctgat aactgaacca ccacaagcta ctctttggag
     1261 cccatctatg gtttgtggta tgaccactcc tccaacttct cctggaaatg tcccacctga
     1321 tctgtcacac ccttacagta aagtctttgg tacaactgca ggtggaaaag gaactcctct
     1381 gggaacccca gcaacctctc ctcctccagc cccactctgt cattcggatg actacgtgca
     1441 catttcactc ccccaggcca cagtcacacc ccccaggaag gaagagagaa tggattctgc
     1501 aagaccatgt ctacacagac aacaccatct tctgaatgac agaggatcag aagagccacc
     1561 tggcagcaaa ggttctgtca ctctaagtga tcttccaggg tttttaggtg atctggcctc
     1621 tgaagaagat agtattgaaa aagataaaga agaagctgca atatctagag aactttctga
     1681 gatcaccaca gcagaggcag agcctgtggt tcctcgagga ggctttgact ctccctttta
     1741 ccgagacagt ctcccaggtt ctcagcggaa gacccactcg gcagcctcca gttctcaggg
     1801 cgccagcgtg aaccctgagc ctttacactc ctccctggac aagcttgggc ctgacacacc
     1861 aaagcaagcc tttactccca tagacctgcc ctgcggcagt gctgatgaaa gccctgcggg
     1921 agacagggaa tgccagactt ctttggagac cagtatcttc actcccagtc cttgtaaaat
     1981 tccacctccg acgagagtgg gctttggaag cgggcagcct cccccgtatg atcatctttt
     2041 tgaggtggca ttgccaaaga cagcccatca ttttgtcatc aggaagactg aggagctgtt
     2101 aaagaaagca aaaggaaaca cagaggaaga tggtgtgccc tctacctccc caatggaagt
     2161 gctggacaga ctgatacagc agggagcaga cgcgcacagc aaggagctga acaagttgcc
     2221 tttacccagc aagtctgtcg actggaccca ctttggaggc tctcctcctt cagatgagat
     2281 ccgcaccctc cgagaccagt tgcttttact gcacaaccag ttactctatg agcgttttaa
     2341 gaggcagcag catgccctcc ggaacaggcg gctcctccgc aaggtgatca aagcagcagc
     2401 tctggaggaa cataatgctg ccatgaaaga tcagttgaag ttacaagaga aggacatcca
     2461 gatgtggaag gttagtctgc agaaagaaca agctagatac aatcagctcc aggagcagcg
     2521 tgacactatg gtaaccaagc tccacagcca gatcagacag ctgcagcatg accgagagga
     2581 attctacaac cagagccagg aattacagac gaagctggag gactgcagga acatgattgc
     2641 ggagctgcgg atagaactga agaaggccaa caacaaggtg tgtcacactg agctgctgct
     2701 cagtcaggtt tcccaaaagc tctcaaacag tgagtcggtc cagcagcaga tggagttctt
     2761 gaacaggcag ctgttggttc ttggggaggt caacgagctc tatttggaac aactgcagaa
     2821 caagcactca gataccacaa aggaagtaga aatgatgaaa gccgcctatc ggaaagagct
     2881 agaaaaaaac agaagccatg ttctccagca gactcagagg cttgatacct cccaaaaacg
     2941 gattttggaa ctggaatctc acctggccaa gaaagaccac cttcttttgg aacagaagaa
     3001 atatctagag gatgtcaaac tccaggcaag aggacagctg caggccgcag agagcaggta
     3061 tgaggctcag aaaaggataa cccaggtgtt tgaattggag atcttagatt tatatggcag
     3121 gttggagaaa gatggcctcc tgaaaaaact tgaagaagaa aaagcagaag cagctgaagc
     3181 agcagaagaa aggcttgact gttgtaatga cgggtgctca gattccatgg tagggcacaa
     3241 tgaagaggca tctggccaca acggtgagac caagaccccc aggcccagca gcgcccgggg
     3301 cagtagtgga agcagaggtg gtggaggcag cagcagcagc agcagcgagc tttctacccc
     3361 agagaaaccc ccacaccaga gggcaggccc attcagcagt cggtgggaga cgactatggg
     3421 agaagcgtct gccagcatcc ccaccactgt gggctcactt cccagttcaa aaagcttcct
     3481 gggtatgaag gctcgagagt tatttcgtaa taagagcgag agccagtgtg atgaggacgg
     3541 catgaccagt agcctttctg agagcctaaa gacagaactg ggcaaagact tgggtgtgga
     3601 agccaagatt cccctgaacc tagatggccc tcacccgtct cccccgaccc cggacagtgt
     3661 tggacagcta catatcatgg actacaatga gactcatcat gaacacagct aaggaatgat
     3721 ggtcaatcag tgttaacttg catattgttg gcacagaaca ggaggtgtga atgcacgttt
     3781 caaagctttc ctgtttccag ggtctgagtg caagttcatg tgtggaaatg ggacggaggt
     3841 cctttggaca gctgactgaa tgcagaacgg tttttggatc tggcattgaa atgcctcttg
     3901 accttcccct ccacccgccc taaccccctc tcatttacct cgcagtgtgt tctaatccaa
     3961 gggccagttg gtgttcctca gtagctttac tttcttcctt tcccccccaa atggttgcgt
     4021 cctttgaacc tgtgcaatat gaggccaaat ttaatctttg agtctaacac accactttct
     4081 gctttcccga agttcagata actgggttgg ctctcaatta gaccaggtag tttgttgcat
     4141 tgcaggtaag tctggttttg tcccttccag gaggacatag cctgcaaagc tggttgtctt
     4201 tacatgaaag cgtttacatg agactttccg actgcttttt tgattctgaa gttcagcatc
     4261 taaagcagca ggtctagaag aacaacggtt tattcatact tgcattcttt tggcagttct
     4321 gataagcttc ctagaaagtt ctgtgtaaac agaagcctgt ttcagaaatc tggagctggc
     4381 actgtggaga ccacacaccc tttgggaaag ctcttgtctc ttcttccccc actacctctt
     4441 atttatttgg tgtttgcttg aatgctggta ctattgtgac cacaggctgg tgtgtaggtg
     4501 gtaaaacctg ttctccatag gagggaagga gcagtcactg ggagaggtta cccgagaagc
     4561 acttgagcat gaggaactgc acctttaggc catctcagct tgctgggcct tttgttaaac
     4621 ccttctgtct actggcctcc ctttgtgtgc atacgcctct tgttcatgtc agcttatatg
     4681 tgacactgca gcagaaaggc tctgaaggtc caaagagttt ctgcaaagtg tatgtgacca
     4741 tcatttccca ggccattagg gttgcctcac tgtagcaggt tctaggctac cagaagaggg
     4801 gcagcttttt cataccaatt ccaactttca ggggctgact ctccagggag ctgatgtcat
     4861 cacactctcc atgttagtaa tggcagagca gtctaaacag agtccgggag aatgctggca
     4921 aaggctggct gtgtataccc actaggctgc cccacgtgct cccgagagat gacactagtc
     4981 agaaaattgg cagtggcaga gaatccaaac tcaacaagtg ctcctgaaag aaacgctaga
     5041 agcctaagaa ctgtggtctg gtgttccagc tgaggcaggg ggatttggta ggaaggagcc
     5101 agtgaacttg gctttcctgt ttctatcttt cattaaaaag aatagaagga ttcagtcata
     5161 aagaggtaaa aaactgtcac ggtacgaaat cttagtgccc acggaggcct cgagcagaga
     5221 gaatgaaagt cttttttttt tttttttttt tttagcatgg caataaatat tctagcatcc
     5281 ctaactaaag gggactagac agttagagac tctgtcaccc tagctatacc agcagaaaac
     5341 ctgttcaggc aggctttctg ggtgtgactg attcccagcc tgtggcaggg cgtggtccca
     5401 actactcagc ctagcacagg ctggcagttg gtactgaatt gtcagatgtg gagtattagt
     5461 gacaccacac atttaattca gctttgtcca aaggaaagct taaaacccaa tacagtctag
     5521 tttcctggtt ccgttttaga aaaggaaaac gtgaacaaac ttagaaaggg aaggaaatcc
     5581 catcagtgaa tcctgaaact ggttttaagt gctttccttc tcctcatgcc caagagatct
     5641 gtgccataga acaagatacc aggcacttaa agccttttcc tgaattggaa aggaaaagag
     5701 gcccaagtgc aaaagaaaaa acattttaga aacggacagc ttataaaaat aaagggaaga
     5761 aaggaggcag catggagaga ggcctgtgct agaagctcca tggacgtgtc tgcacagggt
     5821 cctcagctca tccatgcggc ctgggtgtcc ttttactcag ctttataaca aatgtggctc
     5881 caagctcagg tgcctttgag ttctaggagg ctgtgggttt tattcaacta cggttgggag
     5941 aatgagacct ggagtcatgt tgaaggtgcc caacctaaaa atgtaggctt tcatgttgca
     6001 aagaactcca gagtcagtag ttaggtttgg tttggttttg gacatgataa acctgccaag
     6061 agtcaacagg tcacttgatc atgctgcagt gggtagttct aaggatggaa aggtgacagt
     6121 attactctcg agaggcaatt cagtcctggg caaaggtatt agtacaataa gcgttaaggg
     6181 cagagtctac cttgaaacca attaagcagc ttggtattca taaatattgg gattggatgg
     6241 cctccatcca gaaatcacta tgggtgagca tacctgtctc agctgtttgg ccaatgtgca
     6301 taacctactc ggatccccac ctgacactaa ccagagtcag cacaggcccc gaggagcccg
     6361 aagtctgctg ctgtgcagca tggaattcct ttaaaaaggt gcactacagt tttagcgggg
     6421 agggggatag gaagacgcag agcaaatgag ctccggagtc cctgcaggtg aataaacaca
     6481 cagatctgca tctgatagaa ctttgatgga ttttcaaaaa gccgttgaca aggctctgct
     6541 atacagtcta taaaaattgt tattatggga ttggaagaaa cacgtggtca tgaatagaaa
     6601 aaaaacaaac ccaaaggtag gaaggtcaag gtcatttctt agatggagaa gttgtgaaag
     6661 atgtccttgg agatgagttt taggaccagc attactaagg caggtgggca gacagtgacc
     6721 tctctaggtg tgtccacaga gtttttcagg agagaaaact gcctgacctt tgggactaag
     6781 ctgcggaatc ttcttactaa gcttgaagag tggagaggcg agaggtgagc tactttgtga
     6841 gccaaagctt atgtgacatg gttggggaaa cagtccaaac tgttctgaga aggtgaactg
     6901 ttacgaccca ggacaattag aaaaattcac ccaccatgcc gcacattact gggtaaaagc
     6961 agggcagcag ggaacaaaac tccagactct tgggccgtcc ccatttgcaa cagcacacat
     7021 agtttctggt atatttgttg ggaaagataa aactctagca gttgttgagg ggaggatgta
     7081 taaaatggtc atggggatga aaggatctct gagaccacag aggctcagac tcactgttaa
     7141 gaatagaaaa ctgggtatgc gtttcatgta gccagcagaa ctgaagtgtg ctgtgacaag
     7201 ccaatgtgaa tttctaccaa atagtagagc ataccacttg aagaaggaaa gaaccgaaga
     7261 gcaaacaaaa gttctgcgta atgagactca ccttttctcg ctgaaagcac taagaggtgg
     7321 gaggaggcct gcacaggctg gaggagggtt tgggcagagc gaagacccgg ccaggacctt
     7381 ggtgagatgg ggtgccgccc acctcctgcg gatactcttg gagagttgtt cccccagggg
     7441 gctctgcccc acctggagaa ggaagctgcc tggtgtggag tgactcaaat cagtatacct
     7501 atctgctgca ccttcactct ccagggtaca tgctttaaaa ccgacccgca acaagtattg
     7561 gaaaaatgta tccagtctga agatgtttgt gtatctgttt acatccagag ttctgtgaca
     7621 catgcccccc agattgctgc aaagatccca aggcattgat tgcacttgat taagcttttg
     7681 tctgtaggtg aaagaacaag tttaggtcga ggactggccc ctaggctgct gctgtgaccc
     7741 ttgtcccatg tggcttgttt gcctgtccgg gactcttcga tgtgcccagg ggagcgtgtt
     7801 cctgtctctt ccatgccgtc ctgcagtcct tatctgctcg cctgagggaa gagtagctgt
     7861 agctacaagg gaagcctgcc tggaagagcc gagcacctgt gcccatggct tctggtcatg
     7921 aaacgagtta atgatggcag aggagcttcc tccccacttc gcagcgccac attatccatc
     7981 ctctgagata agtaggctgg tttaaccatt ggaatggacc tttcagtgga aaccctgaga
     8041 gtctgagaac ccccagacca acccttccct ccctttcccc acctcttaca gtgtttggac
     8101 aggagggtat ggtgctgctc tgtgtagcaa gtactttggc ttatgaaaga ggcagccacg
     8161 cattttgcac taggaagaat cagtaatcac ttttcagaag acttctatgg accacaaata
     8221 tattacggag gaacagattt tgctaagaca taatctagtt ttataactca atcatgaatg
     8281 aaccatgtgt ggcaaacttg cagtttaaag gggtcccatc agtgaaagaa actgattttt
     8341 tttaacggac tgcttttagt taaattgaag aaagtcagct cttgtcaaaa ggtctaaact
     8401 ttcccgcctc aatcctaaaa gcatgtcaac aatccacatc agatgccata aatatgaact
     8461 gcaggataaa atggtacaat cttagtgaat gggaattgga atcaaaagag tttgctgtcc
     8521 ttcttagaat gttctaaaat gtcaaggcag ttgcttgtgt ttaactgtga acaaataaaa
     8581 atttattgtt ttgcacta
//
