LOCUS       NM_015679               5426 bp    mRNA    linear   PRI 18-NOV-2024
DEFINITION  Homo sapiens TruB pseudouridine synthase family member 2 (TRUB2),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_015679
VERSION     NM_015679.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Mukhopadhyay,S., Deogharia,M. and Gupta,R.
  TITLE     Mammalian nuclear TRUB1, mitochondrial TRUB2, and cytoplasmic PUS10
            produce conserved pseudouridine 55 in different sets of tRNA
  JOURNAL   RNA 27 (1), 66-79 (2021)
   PUBMED   33023933
  REMARK    GeneRIF: Mammalian nuclear TRUB1, mitochondrial TRUB2, and
            cytoplasmic PUS10 produce conserved pseudouridine 55 in different
            sets of tRNA.
REFERENCE   3  (bases 1 to 5426)
  AUTHORS   Carlile,T.M., Martinez,N.M., Schaening,C., Su,A., Bell,T.A.,
            Zinshteyn,B. and Gilbert,W.V.
  TITLE     mRNA structure determines modification by pseudouridine synthase 1
  JOURNAL   Nat Chem Biol 15 (10), 966-974 (2019)
   PUBMED   31477916
REFERENCE   4  (bases 1 to 5426)
  AUTHORS   Zaganelli,S., Rebelo-Guiomar,P., Maundrell,K., Rozanska,A.,
            Pierredon,S., Powell,C.A., Jourdain,A.A., Hulo,N.,
            Lightowlers,R.N., Chrzanowska-Lightowlers,Z.M., Minczuk,M. and
            Martinou,J.C.
  TITLE     The Pseudouridine Synthase RPUSD4 Is an Essential Component of
            Mitochondrial RNA Granules
  JOURNAL   J Biol Chem 292 (11), 4519-4532 (2017)
   PUBMED   28082677
REFERENCE   5  (bases 1 to 5426)
  AUTHORS   Antonicka,H., Choquet,K., Lin,Z.Y., Gingras,A.C., Kleinman,C.L. and
            Shoubridge,E.A.
  TITLE     A pseudouridine synthase module is essential for mitochondrial
            protein synthesis and cell viability
  JOURNAL   EMBO Rep 18 (1), 28-38 (2017)
   PUBMED   27974379
REFERENCE   6  (bases 1 to 5426)
  AUTHORS   Arroyo,J.D., Jourdain,A.A., Calvo,S.E., Ballarano,C.A.,
            Doench,J.G., Root,D.E. and Mootha,V.K.
  TITLE     A Genome-wide CRISPR Death Screen Identifies Genes Essential for
            Oxidative Phosphorylation
  JOURNAL   Cell Metab 24 (6), 875-885 (2016)
   PUBMED   27667664
REFERENCE   7  (bases 1 to 5426)
  AUTHORS   Floyd,B.J., Wilkerson,E.M., Veling,M.T., Minogue,C.E., Xia,C.,
            Beebe,E.T., Wrobel,R.L., Cho,H., Kremer,L.S., Alston,C.L.,
            Gromek,K.A., Dolan,B.K., Ulbrich,A., Stefely,J.A., Bohl,S.L.,
            Werner,K.M., Jochem,A., Westphall,M.S., Rensvold,J.W., Taylor,R.W.,
            Prokisch,H., Kim,J.P., Coon,J.J. and Pagliarini,D.J.
  TITLE     Mitochondrial Protein Interaction Mapping Identifies Regulators of
            Respiratory Chain Function
  JOURNAL   Mol Cell 63 (4), 621-632 (2016)
   PUBMED   27499296
REFERENCE   8  (bases 1 to 5426)
  AUTHORS   Zucchini,C., Strippoli,P., Biolchi,A., Solmi,R., Lenzi,L.,
            D'Addabbo,P., Carinci,P. and Valvassori,L.
  TITLE     The human TruB family of pseudouridine synthase genes, including
            the Dyskeratosis Congenita 1 gene and the novel member TRUB1
  JOURNAL   Int J Mol Med 11 (6), 697-704 (2003)
   PUBMED   12736709
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DC326376.1, BC001457.2, AL359091.10 and AJ420462.1.
            
            On Nov 23, 2018 this sequence version replaced NM_015679.2.
            
            Summary: Pseudouridine is an abundant component of rRNAs and tRNAs
            and is enzymatically generated by isomerization of uridine by
            pseudouridine synthase (Zucchini et al., 2003 [PubMed
            12736709]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK001956.1, AJ420462.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372890.6/ ENSP00000361982.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                DC326376.1         335-344
            11-1140             BC001457.2         1-1130
            1141-1142           AL359091.10        8639-8640           c
            1143-1431           BC001457.2         1133-1421
            1432-1485           AJ420462.1         1407-1460
            1486-5426           AL359091.10        4355-8295           c
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.11"
     gene            1..5426
                     /gene="TRUB2"
                     /gene_synonym="CLONE24922"
                     /note="TruB pseudouridine synthase family member 2"
                     /db_xref="GeneID:26995"
                     /db_xref="HGNC:HGNC:17170"
                     /db_xref="MIM:610727"
     exon            1..148
                     /gene="TRUB2"
                     /gene_synonym="CLONE24922"
                     /inference="alignment:Splign:2.1.0"
     CDS             40..1035
                     /gene="TRUB2"
                     /gene_synonym="CLONE24922"
                     /EC_number="5.4.99.25"
                     /note="isoform 1 is encoded by transcript variant 1;
                     probable tRNA pseudouridine synthase 2; TruB pseudouridine
                     (psi) synthase family member 2; TruB pseudouridine (psi)
                     synthase homolog 2; mitochondrial mRNA pseudouridine
                     synthase TRUB2; psi55 synthase TRUB2; tRNA pseudouridine
                     55 synthase TRUB2; truB pseudouridine synthase homolog 2;
                     pseudouridylate synthase TRUB2, mitochondrial"
                     /codon_start=1
                     /product="pseudouridylate synthase TRUB2, mitochondrial
                     isoform 1"
                     /protein_id="NP_056494.1"
                     /db_xref="CCDS:CCDS6897.1"
                     /db_xref="GeneID:26995"
                     /db_xref="HGNC:HGNC:17170"
                     /db_xref="MIM:610727"
                     /translation="MGSAGLSRLHGLFAVYKPPGLKWKHLRDTVELQLLKGLNARKPP
                     APKQRVRFLLGPMEGSEEKELTLTATSVPSFINHPLVCGPAFAHLKVGVGHRLDAQAS
                     GVLVLGVGHGCRLLTDMYNAHLTKDYTVRGLLGKATDDFREDGRLVEKTTYDHVTREK
                     LDRILAVIQGSHQKALVMYSNLDLKTQEAYEMAVRGLIRPMNKSPMLITGIRCLYFAP
                     PEFLLEVQCMHETQKELRKLVHEIGLELKTTAVCTQVRRTRDGFFTLDSALLRTQWDL
                     TNIQDAIRAATPQVAAELEKSLSPGLDTKQLPSPGWSWDSQGPSSTLGLERGAGQ"
     exon            149..280
                     /gene="TRUB2"
                     /gene_synonym="CLONE24922"
                     /inference="alignment:Splign:2.1.0"
     exon            281..355
                     /gene="TRUB2"
                     /gene_synonym="CLONE24922"
                     /inference="alignment:Splign:2.1.0"
     exon            356..417
                     /gene="TRUB2"
                     /gene_synonym="CLONE24922"
                     /inference="alignment:Splign:2.1.0"
     exon            418..499
                     /gene="TRUB2"
                     /gene_synonym="CLONE24922"
                     /inference="alignment:Splign:2.1.0"
     exon            500..572
                     /gene="TRUB2"
                     /gene_synonym="CLONE24922"
                     /inference="alignment:Splign:2.1.0"
     exon            573..709
                     /gene="TRUB2"
                     /gene_synonym="CLONE24922"
                     /inference="alignment:Splign:2.1.0"
     exon            710..5426
                     /gene="TRUB2"
                     /gene_synonym="CLONE24922"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1466..1471
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRUB2"
                     /gene_synonym="CLONE24922"
                     /note="hexamer: ATTAAA"
     polyA_site      1485
                     /gene="TRUB2"
                     /gene_synonym="CLONE24922"
                     /note="major polyA site"
     polyA_site      4637
                     /gene="TRUB2"
                     /gene_synonym="CLONE24922"
     regulatory      5415..5420
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRUB2"
                     /gene_synonym="CLONE24922"
                     /note="hexamer: ATTAAA"
     polyA_site      5426
                     /gene="TRUB2"
                     /gene_synonym="CLONE24922"
                     /note="major polyA site"
ORIGIN      
        1 acttccgtcc aggtccagcg ggtgctgtga tcttcaagta tggggtctgc tggcttgtcg
       61 cggctgcatg ggcttttcgc ggtctataag cccccggggc taaaatggaa gcacctgcgg
      121 gatacagtgg agctacaact tctgaagggt ctcaatgcca ggaagcctcc cgctcctaaa
      181 cagcgtgttc gcttcttgct gggccccatg gaaggcagcg aagagaagga gctgaccctc
      241 acagccacca gcgtaccctc tttcatcaac catccactgg tatgtggacc agcattcgcc
      301 catctcaagg ttggcgtggg acatcggttg gatgcccagg cttctggagt acttgtgctc
      361 ggcgtgggac atggatgcag gctcctcacc gatatgtaca atgctcatct taccaaggat
      421 tacacagtgc gtggcctcct gggcaaagct acagatgact tccgtgagga cgggaggctg
      481 gtagagaaga caacctatga ccacgtgacc agagagaagc tggaccgcat tctggccgtt
      541 atccaaggct cccatcagaa ggccctggtg atgtactcca acctcgacct gaagacccag
      601 gaggcctatg agatggccgt gagaggcctg atccggccca tgaacaagtc cccgatgctg
      661 ataactggca tccgatgcct ctactttgca cctccggaat tcctcttaga ggtgcagtgc
      721 atgcatgaga cgcagaaaga gctgcggaag ttggttcatg aaatcggcct ggaactaaag
      781 accactgctg tctgcaccca agtgcggcgc acgcgcgacg gcttcttcac gctagacagt
      841 gccctcctga ggacccagtg ggacctaacc aacatccagg atgctatccg ggctgctacc
      901 cctcaggtag ctgcagagct ggagaagagc ttgagcccgg ggctggacac caagcagctc
      961 cccagtccgg gatggtcctg ggactcccag ggcccgagct ctaccttggg gctggagagg
     1021 ggtgcggggc agtgaatgcc caggcagctc ctggggcagt ggatggataa atagaagaac
     1081 tgctatgagc tggacctgat agctgtgcag aaagtgagag caggggcacc ttttctacgt
     1141 aagacacaaa actcaagctg taacagaaag gtcaatggac ttgacccaac tgagaaggaa
     1201 aaacttctgt atggaggaaa agacagtaaa catgacagac tgatagaaag tattggcaga
     1261 ccaggcgtgg tggcgggcac ttgtaatccc agctactccc aaggctgagg caggagaatt
     1321 gcttgagccc aggagtttaa ggttgtagtg cgctagacgg cgcctgtgaa tagccactgc
     1381 actctagtct ggggaacata tgagacccct gccatctcaa aaaaaaaaaa attctcaata
     1441 aactatgtgc ccacgaaaat taaaaattaa aaaaaattgt aagtatttgc agtgtcatta
     1501 accaaggact aatatccata gtatacagag agcccttaga aagcaataag aagacaacga
     1561 caaaattagg caaatgagat gaagtgtgta tcagaagaaa tccaaatggc tagtaagcca
     1621 gaaatgacac tcaacctctt taatactgga agacacagat gaaaatgaga tgatttttgt
     1681 tgtttttctg ttgttgccca ggctggagtg cagtggcgcc atctttgctc actgcagcct
     1741 ccgcctccca ggttcaagtg attctcctgc ctcagcctcc caagtagctg gggtcacagg
     1801 cgcccgccac cacacctggc taattttttg tatccgagat gaggtttcac catgttggcc
     1861 tggctcagcc tcccaaagtg ctgggattac aggcgtgagc caccgcacct ggccgggacc
     1921 tcagtctttt ctcttaaagg tcttcagttt attgaatgag ctccgcccac aattgtggtg
     1981 gataatcagc gtgactcaaa gtctacccat tgactgattg aaatgttaat catttttttg
     2041 tttttgtttt tgtttttgag acttgctttg tggcccaggc tggagtgcag tggtgcgatc
     2101 taggctcact gcaacctccg cttcccaggt tcacgccatt ctcctgcctc agcctcccga
     2161 gtagctggga ctacaggtgc ccaccaccac gcccagctaa tttttttgta tttttagtag
     2221 agacggtgtt gcaccgtgtt agccaggatg gtctcaatct cctgacctcg tgatccgcct
     2281 ggcttggcct cccaaagtac tgggattaca gccgtgagcc accacacccg gcctgttttg
     2341 gttttttttt tttctgagac ggagtctcac tccgtcatcc aggttggagt gcagtggcac
     2401 aatctcagct cactgcaacc tctgcctccc aggttcaagc gattctcctg cctcagcttc
     2461 tcaagtagct gggattacag atgtgtacca ccatgcctgg ctaatttttg tatttttagt
     2521 agagacaggg tttcaccatg ttggccaggc tggtctggaa ctcctgacct caggtgatcc
     2581 gcccacctca gcctcccaga gcgctgggat tatgccaacc tctgcctccc aaagcactgg
     2641 gatggcaggc atgagccacc acacccggcc tgtttttgtt ttttagacag ggtcttgctc
     2701 tgtcacccag gctggagtgc agtgatgcaa acatgactca ctgcagcttt gtcctctcag
     2761 gcttaaccat cttcccacct cagcttccag aatagctggg actgcaggcg tgcacacagt
     2821 atctggctaa tttatttatt ttattttatt tttgagacag ggcctcgcac tgtcgcccag
     2881 gctagagtgc agtggcgcgg tttcaactca ttgcagcctc gaccctcctg ggtcaagcga
     2941 ttcttcctgc cttagtctcc agagttgctg ggattaccgg tgtgcaccac caagcctggc
     3001 taatttttgt atttttagta gagacagggt ttcactatgt tggccaggct ggtctggaac
     3061 tcctgacctg aggtgatccg cctacctcgg ccttccaaag tactgggaat ataggcatga
     3121 gccactgttt ccggcctgtt ttttttagac agagtcttgc tctgtcaccc aggctggagt
     3181 gcaatggtgc aaacatgact cactgcaccg tcatcctccc aggctcaacc atcttcccac
     3241 ctcaccctcc agaatagctg ggactacagg cgtgcaccac gatgcctggc taaatatttt
     3301 tttccagcta attttattat ttttttttga gatggagtct tgctctgtca cccaggctgg
     3361 agtgcaatgg cacaatcttg gctcactgca acctccgcct cccagggtca agtgattctc
     3421 ctgcctcagc cacccaagta gctgggatta taggcgttca ccaccacgcc cagctaactt
     3481 ttgtattttt agtagagacg gggtttcacc atgttggaca tgctggtctc aaagtcctga
     3541 cctcaggtga tctgcccgct tcagcctccc aaagtgctgg gattacaggc gtgagccacc
     3601 atacctggcc cacctgagat caggagttca agaccagcct ggccaacata gtgaaaccct
     3661 gtctctacta aaaatacaaa aattagccag ctatggtgac gcacttctgt aatcctagct
     3721 acttgggagg ctgaggcaca agaatcgctg aagcccagga ggtggagatt gccattagct
     3781 gagattgtgc ccctgcactc cagtctggac gagagagtga gactctgtct cagaaaagaa
     3841 aaaaaaaaag gctgggcacg gtggctcacg cctgtaatcc cagcactttg ggaggccagg
     3901 gtgggcggat cacgaggtca ggagttcaag accagcctga ccaacatggt gaaacctgtc
     3961 tctactaaaa atacaaaatt agccgggcgt gcttgcacgt gcctgtagtc ctggctaccc
     4021 gggaggctga tgcagaataa ctggaactcg ggaggcggag cttgcagtga gtggagattg
     4081 cgccactgca ctccagccta ggtgacagag tgagactctg tctcaaaaaa aaaaaaaaaa
     4141 gagaaaaatc agaacaaaat cagcaatatt tagacaagca gaatccaatg aaatggtgag
     4201 gaaagctggg tgggagtgga gggtatcagc aaaactcagt tctttggaaa aattaggtaa
     4261 cttgaacgta gggcacatgg gctgtcacac caccccattt agagccctta ttggcataga
     4321 gatgataaaa gtggactctg tgatggcctc tcatgatctc aggatagcag gataggggaa
     4381 ctctgagtca tggccaaaat aattggtcaa tgtacagcct cagacagctg ggaataccac
     4441 cccaaatcac ttggataagg agatgttttc cttatcctgt gtctttaact ctgagacgga
     4501 tggagttgaa caatcaacga ttgttaaaaa tgtaccaaat gtgtaagcat catgaggtgt
     4561 ttaagctgag cttttaaatt aaaattctgc tgggtgtggc ggctcatgcc tgtaatccca
     4621 gcactttgcg atccccacac aggaggattg cttgagccca gaagtttgag accagcctgg
     4681 gcaacacaag gagaccctgt ctctacaaaa aacaaacaaa caaacaaaaa agccgggggt
     4741 gttggtgtgt gcctgtagtc ccagctacta gggaggctga gatgggagga tcgcttgaac
     4801 ctagcaagtc aaggcggcag taaatggaga tctgtactcc agcctgggca acagagcgag
     4861 accctatgtc agaaaaaaag aaaaagccac ccagcaggct gagcgtggtg gctcacgcct
     4921 gtaatccctg cactttggga agccaaagca ggtgaatcac ttgaggtcag gagttcgaga
     4981 ccagcctggc caatatggtg aaaccctgtc tctaccaaaa atacaaaaat tagccgggca
     5041 tggtggcgct tgtctgtagt ccagctacag ctactccaga tctgggctga ggtgggagaa
     5101 tcatttgaac ccaggaggtg gaggttgcag tgagctgaaa tcacacgact gtactctagc
     5161 ctgggtgaca gggtgagacc ctatctcaaa acaaacaaac aaaaacccca ccagcaacct
     5221 tgctgacaaa tccacaggcc cccacgtctg ccaattgcct gcctgtatga actgggcacc
     5281 tgggggaggg gcgtggcccc caggctactc atcttggctt tcacagccaa agggcctggg
     5341 tggaacctca gcttactgtg tttactgact gggtaatgtg gcctctgaag ctgtcaccta
     5401 tctataaaat ggggattaaa acgaca
//
