LOCUS       NM_017672              10382 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens transient receptor potential cation channel subfamily
            M member 7 (TRPM7), transcript variant 1, mRNA.
ACCESSION   NM_017672
VERSION     NM_017672.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10382)
  AUTHORS   Zhao,F., Yu,W., Hu,J., Xia,Y., Li,Y., Liu,S., Liu,A., Wang,C.,
            Zhang,H., Zhang,L. and Shi,J.
  TITLE     Hypoxia-induced TRPM7 promotes glycolytic metabolism and
            progression in hepatocellular carcinoma
  JOURNAL   Eur J Pharmacol 974, 176601 (2024)
   PUBMED   38677534
  REMARK    GeneRIF: Hypoxia-induced TRPM7 promotes glycolytic metabolism and
            progression in hepatocellular carcinoma.
REFERENCE   2  (bases 1 to 10382)
  AUTHORS   Lin,Y., Chen,Y., Hu,W., Liu,X., Hao,W., Xing,J., Ding,J., Xu,Y.,
            Yao,F., Zhao,Y., Wang,K., Li,S., Yu,Q., Hu,W. and Zhou,R.
  TITLE     TRPM7 facilitates fibroblast-like synoviocyte proliferation,
            metastasis and inflammation through increasing IL-6 stability via
            the PKCalpha-HuR axis in rheumatoid arthritis
  JOURNAL   Int Immunopharmacol 132, 111933 (2024)
   PUBMED   38581988
  REMARK    GeneRIF: TRPM7 facilitates fibroblast-like synoviocyte
            proliferation, metastasis and inflammation through increasing IL-6
            stability via the PKCalpha-HuR axis in rheumatoid arthritis.
REFERENCE   3  (bases 1 to 10382)
  AUTHORS   Zhang,P., Guo,E., Xu,L., Shen,Z., Jiang,N. and Liu,X.
  TITLE     Knockdown of circ-Gatad1 alleviates LPS induced HK2 cell injury via
            targeting miR-22-3p/TRPM7 axis in septic acute kidney
  JOURNAL   BMC Nephrol 25 (1), 79 (2024)
   PUBMED   38443846
  REMARK    GeneRIF: Knockdown of circ-Gatad1 alleviates LPS induced HK2 cell
            injury via targeting miR-22-3p/TRPM7 axis in septic acute kidney.
            Erratum:[BMC Nephrol. 2024 Apr 25;25(1):147. doi:
            10.1186/s12882-024-03580-4.. PMID: 38664673]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10382)
  AUTHORS   Fleig,A. and Chubanov,V.
  TITLE     TRPM7.
  JOURNAL   Handb Exp Pharmacol 222, 521-546 (2014)
   PUBMED   24756720
  REMARK    Review article
REFERENCE   5  (bases 1 to 10382)
  AUTHORS   Ryazanova,L.V., Dorovkov,M.V., Ansari,A. and Ryazanov,A.G.
  TITLE     Characterization of the protein kinase activity of TRPM7/ChaK1, a
            protein kinase fused to the transient receptor potential ion
            channel
  JOURNAL   J Biol Chem 279 (5), 3708-3716 (2004)
   PUBMED   14594813
  REMARK    GeneRIF: only Mg(2+) can directly modulate TRPM7/ChaK1 kinase
            activity in vivo
REFERENCE   6  (bases 1 to 10382)
  AUTHORS   Schmitz,C., Perraud,A.L., Johnson,C.O., Inabe,K., Smith,M.K.,
            Penner,R., Kurosaki,T., Fleig,A. and Scharenberg,A.M.
  TITLE     Regulation of vertebrate cellular Mg2+ homeostasis by TRPM7
  JOURNAL   Cell 114 (2), 191-200 (2003)
   PUBMED   12887921
REFERENCE   7  (bases 1 to 10382)
  AUTHORS   Runnels,L.W., Yue,L. and Clapham,D.E.
  TITLE     The TRPM7 channel is inactivated by PIP(2) hydrolysis
  JOURNAL   Nat Cell Biol 4 (5), 329-336 (2002)
   PUBMED   11941371
REFERENCE   8  (bases 1 to 10382)
  AUTHORS   Nadler,M.J., Hermosura,M.C., Inabe,K., Perraud,A.L., Zhu,Q.,
            Stokes,A.J., Kurosaki,T., Kinet,J.P., Penner,R., Scharenberg,A.M.
            and Fleig,A.
  TITLE     LTRPC7 is a Mg.ATP-regulated divalent cation channel required for
            cell viability
  JOURNAL   Nature 411 (6837), 590-595 (2001)
   PUBMED   11385574
  REMARK    Erratum:[Nature 2001 Aug 9;412(6847):660]
REFERENCE   9  (bases 1 to 10382)
  AUTHORS   Runnels,L.W., Yue,L. and Clapham,D.E.
  TITLE     TRP-PLIK, a bifunctional protein with kinase and ion channel
            activities
  JOURNAL   Science 291 (5506), 1043-1047 (2001)
   PUBMED   11161216
REFERENCE   10 (bases 1 to 10382)
  AUTHORS   Ryazanov,A.G., Pavur,K.S. and Dorovkov,M.V.
  TITLE     Alpha-kinases: a new class of protein kinases with a novel
            catalytic domain
  JOURNAL   Curr Biol 9 (2), R43-R45 (1999)
   PUBMED   10021370
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP354541.1, AY032950.1,
            AC084756.3 and BC028843.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_017672.5.
            
            Summary: This gene belongs to the melastatin subfamily of transient
            receptor potential family of ion channels. The protein encoded by
            this gene is both an ion channel and a serine/threonine protein
            kinase. The kinase activity is essential for the ion channel
            function, which serves to increase intracellular calcium levels and
            to help regulate magnesium ion homeostasis. The encoded protein is
            involved in cytoskeletal organization, cell adhesion, cell
            migration and organogenesis. Defects in this gene are a cause of
            amyotrophic lateral sclerosis-parkinsonism/dementia complex of
            Guam. The gene may also be associated with defects of cardiac
            function. [provided by RefSeq, Aug 2017].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY032950.1, SRR11853564.9598.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000646667.1/ ENSP00000495860.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                BP354541.1         19-59
            42-5249             AY032950.1         49-5256
            5250-5250           AC084756.3         51908-51908         c
            5251-6097           AY032950.1         5258-6104
            6098-10338          AC084756.3         34225-38465         c
            10339-10382         BC028843.1         534-577
FEATURES             Location/Qualifiers
     source          1..10382
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q21.2"
     gene            1..10382
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /note="transient receptor potential cation channel
                     subfamily M member 7"
                     /db_xref="GeneID:54822"
                     /db_xref="HGNC:HGNC:17994"
                     /db_xref="MIM:605692"
     exon            1..267
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     CDS             265..5862
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /EC_number="2.7.11.1"
                     /note="isoform a is encoded by transcript variant 1; LTRPC
                     ion channel family member 7; transient receptor
                     potential-phospholipase C-interacting kinase;
                     channel-kinase 1; long transient receptor potential
                     channel 7"
                     /codon_start=1
                     /product="transient receptor potential cation channel
                     subfamily M member 7 isoform a"
                     /protein_id="NP_060142.3"
                     /db_xref="CCDS:CCDS42035.1"
                     /db_xref="GeneID:54822"
                     /db_xref="HGNC:HGNC:17994"
                     /db_xref="MIM:605692"
                     /translation="MSQKSWIESTLTKRECVYIIPSSKDPHRCLPGCQICQQLVRCFC
                     GRLVKQHACFTASLAMKYSDVKLGDHFNQAIEEWSVEKHTEQSPTDAYGVINFQGGSH
                     SYRAKYVRLSYDTKPEVILQLLLKEWQMELPKLVISVHGGMQKFELHPRIKQLLGKGL
                     IKAAVTTGAWILTGGVNTGVAKHVGDALKEHASRSSRKICTIGIAPWGVIENRNDLVG
                     RDVVAPYQTLLNPLSKLNVLNNLHSHFILVDDGTVGKYGAEVRLRRELEKTINQQRIH
                     ARIGQGVPVVALIFEGGPNVILTVLEYLQESPPVPVVVCEGTGRAADLLAYIHKQTEE
                     GGNLPDAAEPDIISTIKKTFNFGQNEALHLFQTLMECMKRKELITVFHIGSDEHQDID
                     VAILTALLKGTNASAFDQLILTLAWDRVDIAKNHVFVYGQQWLVGSLEQAMLDALVMD
                     RVAFVKLLIENGVSMHKFLTIPRLEELYNTKQGPTNPMLFHLVRDVKQGNLPPGYKIT
                     LIDIGLVIEYLMGGTYRCTYTRKRFRLIYNSLGGNNRRSGRNTSSSTPQLRKSHESFG
                     NRADKKEKMRHNHFIKTAQPYRPKIDTVMEEGKKKRTKDEIVDIDDPETKRFPYPLNE
                     LLIWACLMKRQVMARFLWQHGEESMAKALVACKIYRSMAYEAKQSDLVDDTSEELKQY
                     SNDFGQLAVELLEQSFRQDETMAMKLLTYELKNWSNSTCLKLAVSSRLRPFVAHTCTQ
                     MLLSDMWMGRLNMRKNSWYKVILSILVPPAILLLEYKTKAEMSHIPQSQDAHQMTMDD
                     SENNFQNITEEIPMEVFKEVRILDSNEGKNEMEIQMKSKKLPITRKFYAFYHAPIVKF
                     WFNTLAYLGFLMLYTFVVLVQMEQLPSVQEWIVIAYIFTYAIEKVREIFMSEAGKVNQ
                     KIKVWFSDYFNISDTIAIISFFIGFGLRFGAKWNFANAYDNHVFVAGRLIYCLNIIFW
                     YVRLLDFLAVNQQAGPYVMMIGKMVANMFYIVVIMALVLLSFGVPRKAILYPHEAPSW
                     TLAKDIVFHPYWMIFGEVYAYEIDVCANDSVIPQICGPGTWLTPFLQAVYLFVQYIIM
                     VNLLIAFFNNVYLQVKAISNIVWKYQRYHFIMAYHEKPVLPPPLIILSHIVSLFCCIC
                     KRRKKDKTSDGPKLFLTEEDQKKLHDFEEQCVEMYFNEKDDKFHSGSEERIRVTFERV
                     EQMCIQIKEVGDRVNYIKRSLQSLDSQIGHLQDLSALTVDTLKTLTAQKASEASKVHN
                     EITRELSISKHLAQNLIDDGPVRPSVWKKHGVVNTLSSSLPQGDLESNNPFHCNILMK
                     DDKDPQCNIFGQDLPAVPQRKEFNFPEAGSSSGALFPSAVSPPELRQRLHGVELLKIF
                     NKNQKLGSSSTSIPHLSSPPTKFFVSTPSQPSCKSHLETGTKDQETVCSKATEGDNTE
                     FGAFVGHRDSMDLQRFKETSNKIKILSNNNTSENTLKRVSSLAGFTDCHRTSIPVHSK
                     QAEKISRRPSTEDTHEVDSKAALIPDWLQDRPSNREMPSEEGTLNGLTSPFKPAMDTN
                     YYYSAVERNNLMRLSQSIPFTPVPPRGEPVTVYRLEESSPNILNNSMSSWSQLGLCAK
                     IEFLSKEEMGGGLRRAVKVQCTWSEHDILKSGHLYIIKSFLPEVVNTWSSIYKEDTVL
                     HLCLREIQQQRAAQKLTFAFNQMKPKSIPYSPRFLEVFLLYCHSAGQWFAVEECMTGE
                     FRKYNNNNGDEIIPTNTLEEIMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKAE
                     EKRSCDMVFGPANLGEDAIKNFRAKHHCNSCCRKLKLPDLKRNDYTPDKIIFPQDEPS
                     DLNLQPGNSTKESESTNSVRLML"
     exon            268..347
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            348..386
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            387..585
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            586..799
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            800..924
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1096
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1271
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..1395
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1396..1468
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1569
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1704
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..1758
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1759..1899
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1900..2034
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2035..2315
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2316..2544
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2545..2700
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2701..2844
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2845..2973
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2974..3252
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            3253..3427
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            3428..3554
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            3555..3739
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            3740..3872
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            3873..4588
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            4589..4653
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            4654..4750
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            4751..4821
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            4822..4856
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            4857..4882
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            4883..4933
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            4934..4999
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            5000..5283
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            5284..5366
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            5367..5572
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            5573..5624
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            5625..5731
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            5732..10382
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7227..7232
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /note="hexamer: AATAAA"
     polyA_site      7247
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /note="major polyA site"
     regulatory      10357..10362
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /note="hexamer: AATAAA"
     polyA_site      10382
                     /gene="TRPM7"
                     /gene_synonym="ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7;
                     TRP-PLIK"
                     /note="major polyA site"
ORIGIN      
        1 gtccccagcc ccgtcgccgg cggaggcggg cgcgggcgcg tccctgtggc cagtcacccg
       61 gaggagttgg tcgcacaatt atgaaagact cggcttctgc tgctagcgcc ggagctgagt
      121 tagttctgag aaggtttccc tgggcgttcc ttgtccggcg gcctctgctg ccgcctccgg
      181 agacgcttcc cgatagatgg ctacaggccg cggaggagga ggaggtggag ttgctgccct
      241 tccggagtcc gccccgtgag gagaatgtcc cagaaatcct ggatagaaag cactttgacc
      301 aagagggaat gtgtatatat tataccaagt tccaaggacc ctcacagatg ccttccagga
      361 tgtcaaattt gtcagcaact cgtcaggtgt ttttgtggtc gcttggtcaa gcaacatgct
      421 tgttttactg caagtcttgc catgaaatac tcagatgtga aattgggtga ccattttaat
      481 caggcaatag aagaatggtc tgtggaaaag catacagaac agagcccaac ggatgcttat
      541 ggagtcataa attttcaagg gggttctcat tcctacagag ctaagtatgt gaggctatca
      601 tatgacacca aacctgaagt cattctgcaa cttctgctta aagaatggca aatggagtta
      661 cccaaacttg ttatctctgt acatgggggc atgcagaaat ttgagcttca cccacgaatc
      721 aagcagttgc ttggaaaagg tcttattaaa gctgcagtta caactggagc ctggatttta
      781 actggaggag taaacacagg tgtggcaaaa catgttggag atgccctcaa agaacatgct
      841 tccagatcat ctcgaaagat ttgcactatc ggaatagctc catggggagt gattgaaaac
      901 agaaatgatc ttgttgggag agatgtggtt gctccttatc aaaccttatt gaaccccctg
      961 agcaaattga atgttttgaa taatctgcat tcccatttca tattggtgga tgatggcact
     1021 gttggaaagt atggggcgga agtcagactg agaagagaac ttgaaaaaac tattaatcag
     1081 caaagaattc atgctaggat tggccagggt gtccctgtgg tggcacttat atttgagggt
     1141 gggccaaatg ttatcctcac agttcttgaa taccttcagg aaagcccccc tgttccagta
     1201 gttgtgtgtg aaggaacagg cagagctgca gatctgctag cgtatattca taaacaaaca
     1261 gaagaaggag ggaatcttcc tgatgcagca gagcccgata ttatttccac tatcaaaaaa
     1321 acatttaact ttggccagaa tgaagcactt catttatttc aaacactgat ggagtgcatg
     1381 aaaagaaagg agcttatcac tgttttccat attgggtcag atgaacatca agatatagat
     1441 gtagcaatac ttactgcact gctaaaaggt actaatgcat ctgcatttga ccagcttatc
     1501 cttacattgg catgggatag agttgacatt gccaaaaatc atgtatttgt ttatggacag
     1561 cagtggctgg ttggatcctt ggaacaagct atgcttgatg ctcttgtaat ggatagagtt
     1621 gcatttgtaa aacttcttat tgaaaatgga gtaagcatgc ataaattcct taccattccg
     1681 agactggaag aactttacaa cactaaacaa ggtccaacta atccaatgct gtttcatctt
     1741 gttcgagacg tcaaacaggg aaatcttcct ccaggatata agatcactct gattgatata
     1801 ggacttgtta ttgaatatct catgggagga acctacagat gcacctatac taggaaacgt
     1861 tttcgattaa tatataatag tcttggtgga aataatcgga ggtctggccg aaatacctcc
     1921 agcagcactc ctcagttgcg aaagagtcat gaatcttttg gcaatagggc agataaaaag
     1981 gaaaaaatga ggcataacca tttcattaag acagcacagc cctaccgacc aaagattgat
     2041 acagttatgg aagaaggaaa gaagaaaaga accaaagatg aaattgtaga cattgatgat
     2101 ccagaaacca agcgctttcc ttatccactt aatgaacttt taatttgggc ttgccttatg
     2161 aagaggcagg tcatggcccg ttttttatgg caacatggtg aagaatcaat ggctaaagca
     2221 ttagttgcct gtaagatcta tcgttcaatg gcatatgaag caaagcagag tgacctggta
     2281 gatgatactt cagaagaact aaaacagtat tccaatgatt ttggtcagtt ggccgttgaa
     2341 ttattagaac agtccttcag acaagatgaa accatggcta tgaaattgct cacttatgaa
     2401 ctgaagaact ggagtaattc aacctgcctt aagttagcag tttcttcaag acttagacct
     2461 tttgtagctc acacctgtac acaaatgttg ttatctgata tgtggatggg aaggctgaat
     2521 atgaggaaaa attcctggta caaggtcata ctaagcattt tagttccacc tgccatattg
     2581 ctgttagagt ataaaactaa ggctgaaatg tcccatatcc cacaatctca agatgctcat
     2641 cagatgacaa tggatgacag cgaaaacaac tttcagaaca taacagaaga gatccccatg
     2701 gaagtgttta aagaagtacg gattttggat agtaatgaag gaaagaatga gatggagata
     2761 caaatgaaat caaaaaagct tccaattacg cgaaagtttt atgcctttta tcatgcacca
     2821 attgtaaaat tctggtttaa cacgttggca tatttaggat ttctgatgct ttatacattt
     2881 gtggttcttg tacaaatgga acagttacct tcagttcaag aatggattgt tattgcttat
     2941 atttttactt atgccattga gaaagtccgt gagatcttta tgtctgaagc tgggaaagta
     3001 aaccagaaga ttaaagtatg gtttagtgat tacttcaaca tcagtgatac aattgccata
     3061 atttctttct tcattggatt tggactaaga tttggagcaa aatggaactt tgcaaatgca
     3121 tatgataatc atgtttttgt ggctggaaga ttaatttact gtcttaacat aatattttgg
     3181 tatgtgcgtt tgctagattt tctagctgta aatcaacagg caggacctta tgtaatgatg
     3241 attggaaaaa tggtggccaa tatgttctac attgtagtga ttatggctct tgtattactt
     3301 agttttggtg ttcccagaaa ggcaatactt tatcctcatg aagcaccatc ttggactctt
     3361 gctaaagata tagtttttca cccatactgg atgatttttg gtgaagttta tgcatacgaa
     3421 attgatgtgt gtgcaaatga ttctgttatc cctcaaatct gtggtcctgg gacgtggttg
     3481 actccatttc ttcaagcagt ctacctcttt gtacagtata tcattatggt taatcttctt
     3541 attgcatttt tcaacaatgt gtatttacaa gtgaaggcaa tttccaatat tgtatggaag
     3601 taccagcgtt atcattttat tatggcttat catgagaaac cagttctgcc tcctccactt
     3661 atcattctta gccatatagt ttctctgttt tgctgcatat gtaagagaag aaagaaagat
     3721 aagacttccg atggaccaaa acttttctta acagaagaag atcaaaagaa acttcatgat
     3781 tttgaagagc agtgtgttga aatgtatttc aatgaaaaag atgacaaatt tcattctggg
     3841 agtgaagaga gaattcgtgt cacttttgaa agagtggaac agatgtgcat tcagattaaa
     3901 gaagttggag atcgtgtcaa ctacataaaa agatcattac aatcattaga ttctcaaatt
     3961 ggccatttgc aagatctttc agccctgacg gtagatacat taaaaacact cactgcccag
     4021 aaagcgtcgg aagctagcaa agttcataat gaaatcacac gagaactgag catttccaaa
     4081 cacttggctc aaaaccttat tgatgatggt cctgtaagac cttctgtatg gaaaaagcat
     4141 ggtgttgtaa atacacttag ctcctctctt cctcaaggtg atcttgaaag taataatcct
     4201 tttcattgta atattttaat gaaagatgac aaagatcccc agtgtaatat atttggtcaa
     4261 gacttacctg cagtacccca gagaaaagaa tttaattttc cagaggctgg ttcctcttct
     4321 ggtgccttat tcccaagtgc tgtttcccct ccagaactgc gacagagact acatggggta
     4381 gaactcttaa aaatatttaa taaaaatcaa aaattaggca gttcatctac tagcatacca
     4441 catctgtcat ccccaccaac caaatttttt gttagtacac catctcagcc aagttgcaaa
     4501 agccacttgg aaactggaac caaagatcaa gaaactgttt gctctaaagc tacagaagga
     4561 gataatacag aatttggagc atttgtagga cacagagata gcatggattt acagaggttt
     4621 aaagaaacat caaacaagat aaaaatacta tccaataaca atacttctga aaacactttg
     4681 aaacgagtga gttctcttgc tggatttact gactgtcaca gaacttccat tcctgttcat
     4741 tcaaaacaag cagaaaaaat cagtagaagg ccatctaccg aagacactca tgaagtagat
     4801 tccaaagcag ctttaatacc ggattggtta caagatagac catcaaacag agaaatgcca
     4861 tctgaagaag gaacattaaa tggtctcact tctccattta agccagctat ggatacaaat
     4921 tactattatt cagctgtgga aagaaataac ttgatgaggt tatcacagag cattccattt
     4981 acacctgtgc ctccaagagg ggagcctgtc acagtgtatc gtttggaaga gagttcaccc
     5041 aacatactaa ataacagcat gtcttcttgg tcacaactag gcctctgtgc caaaatagag
     5101 tttttaagca aagaggagat gggaggaggt ttacgaagag ctgtcaaagt acagtgtacc
     5161 tggtcagaac atgatatcct caaatcaggg catctttata ttatcaaatc ttttcttcca
     5221 gaggtggtta atacatggtc aagtatttac aaagaagata cagttctgca tctctgtctg
     5281 agagaaattc aacaacagag agcagcacaa aagcttacgt ttgcctttaa tcaaatgaaa
     5341 cccaaatcca taccatattc tccaaggttc cttgaagttt tcctgctgta ttgccattca
     5401 gcaggacagt ggtttgctgt ggaagaatgt atgactggag aatttagaaa atacaacaat
     5461 aataatggag atgagattat tccaactaat actctggaag agatcatgct agcctttagc
     5521 cactggactt acgaatatac aagaggggag ttactggtac ttgatttgca aggtgttggt
     5581 gaaaatttga ctgacccatc tgtgataaaa gcagaagaaa agagatcctg tgatatggtt
     5641 tttggcccag caaatctagg agaagatgca attaaaaact tcagagcaaa acatcactgt
     5701 aattcttgct gtagaaagct taaacttcca gatctgaaga ggaatgatta tacgcctgat
     5761 aaaattatat ttcctcagga tgagccttca gatttgaatc ttcagcctgg aaattccacc
     5821 aaagaatcag aatcaactaa ttctgttcgt ctgatgttat aatattaata ttactgaatc
     5881 attggttttg cctgcacctc acagaaatgt tactgtgtca cttttccctc gggaggaaat
     5941 tgtttggtaa tatagaaagg tgtatgcaag ttgaatttgc tgactccagc acagttaaaa
     6001 ggtcaatatt cttttgacct gattaatcag tcagaaagtc cctataggat agagctggca
     6061 gctgagaaat tttaaaggta attgataatt agtatttata actttttaaa gggctctttg
     6121 tatagcagag gatctcattt gactttgttt tgatgagggt gatgctctct cttatgtggt
     6181 acaataccat taaccaaagg taggtgtcca tgcagatttt attggcagct gttttattgc
     6241 cattcaacta gggaaatgaa gaaatcacgc agccttttgg ttaaatggca gtcaaaattt
     6301 tcctcagtgt atttagtgtg ttcagtgatg atatcactgg ttcccaacta gatgcttgtt
     6361 ggccacggga agggaaatga cttgttctaa ttctaggttc acagaggtat gagaagcctg
     6421 aactgaagac cattttcaag agggacggta tttatgaatc agggttaggc tccatattta
     6481 aagatagagc cagttttttt ttttaaatag aacccaaatt gtgtaaaaat gttaattggg
     6541 ttttttaaac attgttttat caagtcactg ttaagtagaa gaaagccatg gtaaactgat
     6601 acataaccta aattataaaa gcagaaacct aactcactcg tcaagggaag ttaccttttg
     6661 aggaaagtta aagtactttt ttccctatct gtatctatag caacaaccca gaacttacaa
     6721 acttctccaa agattttatt gattgttata tcaaatcaga atgtaaacat gaactcttgc
     6781 atatatttaa aattgtgttg gaacatttga acatgaatgc tgtttgtggt acttaagaaa
     6841 ttaattcagt tggattatca ttatgtgata ctggcagatt gcagtgcaac cttatgccaa
     6901 taaaatgtaa tttaacagcc ccagatattg ttgaatattc aacaataaca agaaaagctt
     6961 ttcatctaag ttttatgctt taattttttt tctttttttt tctttttctt ttgtttcctt
     7021 ggtactaatt ttaattttta tttggaaggg agcagtataa agcttatttg tatttagtag
     7081 tgtatctcat agatacagac aaggcaagag atgataagct gtttaaatag tgtttaatat
     7141 tgattggggg tggggagaaa gaaaaagtgt attacttaaa gatactatat acgttttgta
     7201 tatcattaaa tctttaaaag aaatgaaata aatttattgt ttacagatgt ttagtgagtt
     7261 taatcattct gaaaaattat ctgacatttt cagggtgtca atttgagtat cagttttttt
     7321 aaatgaacca tttgtatacc tgtgcttttg atctcctgtc ctgtacaatg tttaaattaa
     7381 tactgatttc ttactgtctt cttagaaatc tgttttttgt taggccaaaa aagggcaata
     7441 tgggctgtct gttgattttt aattttatat tgattatttt cacaggatta taatagtagc
     7501 tatacttttt tttttttttt tttttttgag acggagtctc gctctgttgc ttgggctgga
     7561 gtgcagtggt gcgatctcag ctcaccacaa ccgccgcctt ccgggtttaa gtgattctcc
     7621 tgcctcagcc tcccgagtag ctgggactac aggcacacgc caccatgccc agctaatttt
     7681 tatattttta gtagagacag ggtttcacta tgttggccag tgtggtcaca aactcctgac
     7741 cttgtgagcc accgcacctg gctgctaaca cttatttagt gcctactgtg taccagacat
     7801 tactctaagt atttcacata tattaaccta cttaatcctt ataacaatgt tataaagaaa
     7861 taggtgttat tatcctgttt tgcagatttg aaagtcaagg tgctagagag gtaaagtaac
     7921 gtccataaga ttcttacgtt tatttaataa taagtagcaa cggtaggatt tgaacccagg
     7981 ctggctgcct ttcatctata ctgtttttgt tttgttttgt tttgttttgt tttgttttgt
     8041 ttgtcttggt ggggcatggt ggctcatgcc tgtaatccca gcacttcggg aggccaaggc
     8101 aggtggatca cttgggctca ggagtttgag accagcctgg gcaacatggc aaaatcctat
     8161 ctctgctaaa aaaaaaaata caaaaattag gccaggtgca gtggctcatg cctgtaatcc
     8221 cagcactttg ggaggccaag gtgggcggat cacaaggtca ggagttcgag accagcctga
     8281 ccaacatagt gaaaccccgt ctctactaaa aatacaaaaa attagctggg catggcggtg
     8341 agtgcctgta atcccagcta ctcaggagtc tgaggcagga gaattgcttg aacctgggag
     8401 gtggaggttg cagtgagctg agatcgtgcc attgcgctcc agcctgggca acagtgcgag
     8461 actccgtcaa aaaaaaaaaa ataactggat gtgatggtgt gcacctgtag ttccagctac
     8521 ttgggagact gaggtgggag gatcacttga gcctgggaga ctgaggcagc agtgagctga
     8581 gatcatgcca ctgctttcca acctgggcaa cagagtgaga tcctgtctca gaaagaaaaa
     8641 aaaaaaaaag acaacctctt gctctgttgc ccaggctgga gtgtagtagc gtgatcatag
     8701 ctcactgcag ccgtaaactc ctgggctcaa gcaatcctcc tgccactgcc tcttgattag
     8761 gtggaaccac aggcatgcac caccacacgt acctaatttt atatatatat ttttttattt
     8821 ttcattttta tttatttttg tttttttgag ttgaagtctc actctgttgc ccaggccgga
     8881 gtacagtggc acaatcttgg ctcactgcaa cctctgcctc ccaagatcaa gcaattctcg
     8941 tgcttcagcc tccaaagtag ctgagattac aggtacccac cataatgcct ggctgatttt
     9001 tgtatttttc gtagagacaa ggtttcacct tgttggccag gctgatctca aactcctgac
     9061 ctcaagtgat ccacctcccc cggctaccca aagtactggg attataggtg tgagccacca
     9121 tgcctgggta acacccaact aattttaaat atatattttg tagagatggg gtctagcctt
     9181 gttgcccacg ctggtctcaa attcctgggc tcaagtgatc ctctcgcctg agcttcccaa
     9241 agtggtagaa ttgcaggcat gaattgctgc acccagcctc atctgtgctg tgaattatgt
     9301 gctgtattga ctctcaagca tgatgaccat tggtggtttc tgtaccattt cctgttactt
     9361 tactgaaaca cacctactcc attaacttct tgggttaagt ctagaaagta acagtttact
     9421 tgtaaaccac atttcttatc cccaataagt atttttttaa gattattaaa gttcattatt
     9481 actaccctat gatgtgaaag tgtcatttgc ttaatctttt taatttttta ttctcaacct
     9541 catcttactg aagagaataa aactctttta ccatattctt aaaatgtgga attctcggcc
     9601 aggtgcagtg gctcacgcct gtaattccat cactttggga ggccaaggtg ggtggatcat
     9661 ctgaggtcag gagttcaaga ccagcctggc caacatggtg aaaccccgtc tctactaaaa
     9721 atacaaaaat tatctgggtg tggtggcgcg tgcctgtagg cccagctact caggaggctg
     9781 aggcaggaga attgcttgaa cccaagaggt ggaggttgca gtgagcctag attgctgcca
     9841 ctgcactcca gcctgggtga cagcagaact ctgtctcaaa aaaaagatgt ggaattcttt
     9901 tctgcaaatg ttctctaata gtataccttc ttcagtctgt cgatatatgt atgctattat
     9961 tttacaagta atacatgttg attgtattgg aaattataga aaagattata ttggattgtt
    10021 tagaaaatat ttttaaatgt gaagaaaaat ataaaaatta ctcccttgtt ccactttccc
    10081 cactctcaag tcagactatg ttgttttcat agttagtagc tagcagtcta ccccactaga
    10141 ttatatgctt cacagaggga agggaccctc aagacttcac tggattgagt agcacccaat
    10201 accttgcttg ctgcctggtt tgtgatgggc atactgtaag aaaaaaaaat ctgaatgaca
    10261 aaatgttttt ccataatacc agacttcctc ttgaagagat gggtcgtaat gttgtagtct
    10321 tacatgctta cgtagacaat caaagcaaga atactcaata aatggctatt taccacttga
    10381 aa
//
