LOCUS       NM_017662               8252 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens transient receptor potential cation channel subfamily
            M member 6 (TRPM6), transcript variant a, mRNA.
ACCESSION   NM_017662
VERSION     NM_017662.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Huang,S., Ge,Y., Li,Y., Cui,N., Tan,L., Guo,S., Wang,S., Hao,L.,
            Lei,G. and Yang,X.
  TITLE     Magnesium Status, Genetic Variants of Magnesium-Related Ion Channel
            Transient Receptor Potential Membrane Melastatin 6 (TRPM6) and the
            Risk of Gestational Diabetes Mellitus in Chinese Pregnant Women: A
            Nested Case-Control Study
  JOURNAL   Mol Nutr Food Res 67 (22), e2200835 (2023)
   PUBMED   37759402
  REMARK    GeneRIF: Magnesium Status, Genetic Variants of Magnesium-Related
            Ion Channel Transient Receptor Potential Membrane Melastatin 6
            (TRPM6) and the Risk of Gestational Diabetes Mellitus in Chinese
            Pregnant Women: A Nested Case-Control Study.
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   Kim,E.Y. and Lee,J.M.
  TITLE     Transcriptional Control of Trpm6 by the Nuclear Receptor FXR
  JOURNAL   Int J Mol Sci 23 (4), 1980 (2022)
   PUBMED   35216094
  REMARK    GeneRIF: Transcriptional Control of Trpm6 by the Nuclear Receptor
            FXR.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8252)
  AUTHORS   Andriule,I., Pangonyte,D., Almanaityte,M., Patamsyte,V.,
            Kupryte,M., Karciauskas,D., Mubagwa,K. and Macianskiene,R.
  TITLE     Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes
            from all four chamber walls of the human heart
  JOURNAL   Sci Rep 11 (1), 15445 (2021)
   PUBMED   34326388
  REMARK    GeneRIF: Evidence for the expression of TRPM6 and TRPM7 in
            cardiomyocytes from all four chamber walls of the human heart.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8252)
  AUTHORS   Zouharova,M., Herman,P., Hofbauerova,K., Vondrasek,J. and
            Bousova,K.
  TITLE     TRPM6 N-Terminal CaM- and S100A1-Binding Domains
  JOURNAL   Int J Mol Sci 20 (18), 4430 (2019)
   PUBMED   31505788
  REMARK    GeneRIF: data unambiguously confirm that CaM and S100A1 share the
            same binding domain at the TRPM6 N-terminus although the
            ligand-binding mechanism is different.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8252)
  AUTHORS   Tan,J., Yan,T., Li,Z., Huang,J. and Cai,R.
  TITLE     [Analysis of TRPM6 gene variant in a pedigree affected with
            hypocalcemia secondary to hypomagnesemia]
  JOURNAL   Zhonghua Yi Xue Yi Chuan Xue Za Zhi 36 (8), 805-808 (2019)
   PUBMED   31400133
  REMARK    GeneRIF: The homozygous c.3311C>T (p.Pro1104Leu) variant of the
            TRPM6 gene probably underlies hypocalcemia secondary to
            hypomagnesemia in this pedigree.
REFERENCE   6  (bases 1 to 8252)
  AUTHORS   Walder,R.Y., Landau,D., Meyer,P., Shalev,H., Tsolia,M.,
            Borochowitz,Z., Boettger,M.B., Beck,G.E., Englehardt,R.K., Carmi,R.
            and Sheffield,V.C.
  TITLE     Mutation of TRPM6 causes familial hypomagnesemia with secondary
            hypocalcemia
  JOURNAL   Nat Genet 31 (2), 171-174 (2002)
   PUBMED   12032570
  REMARK    GeneRIF: Individuals with TRPM6 mutations have abnormal renal
            magnesium excretion. Deficiency causes hypomagnesemia with
            secondary hypocalcemia.
REFERENCE   7  (bases 1 to 8252)
  AUTHORS   Schlingmann,K.P., Weber,S., Peters,M., Niemann Nejsum,L.,
            Vitzthum,H., Klingel,K., Kratz,M., Haddad,E., Ristoff,E.,
            Dinour,D., Syrrou,M., Nielsen,S., Sassen,M., Waldegger,S.,
            Seyberth,H.W. and Konrad,M.
  TITLE     Hypomagnesemia with secondary hypocalcemia is caused by mutations
            in TRPM6, a new member of the TRPM gene family
  JOURNAL   Nat Genet 31 (2), 166-170 (2002)
   PUBMED   12032568
  REMARK    GeneRIF: Deficiency /mutations of TRPM6 causes hypomagnesemia with
            secondary hypocalcemia
REFERENCE   8  (bases 1 to 8252)
  AUTHORS   Riazanova,L.V., Pavur,K.S., Petrov,A.N., Dorovkov,M.V. and
            Riazanov,A.G.
  TITLE     [Novel type of signaling molecules: protein kinases covalently
            linked to ion channels]
  JOURNAL   Mol Biol (Mosk) 35 (2), 321-332 (2001)
   PUBMED   11357414
REFERENCE   9  (bases 1 to 8252)
  AUTHORS   Ryazanov,A.G., Pavur,K.S. and Dorovkov,M.V.
  TITLE     Alpha-kinases: a new class of protein kinases with a novel
            catalytic domain
  JOURNAL   Curr Biol 9 (2), R43-R45 (1999)
   PUBMED   10021370
REFERENCE   10 (bases 1 to 8252)
  AUTHORS   Walder,R.Y., Shalev,H., Brennan,T.M., Carmi,R., Elbedour,K.,
            Scott,D.A., Hanauer,A., Mark,A.L., Patil,S., Stone,E.M. and
            Sheffield,V.C.
  TITLE     Familial hypomagnesemia maps to chromosome 9q, not to the X
            chromosome: genetic linkage mapping and analysis of a balanced
            translocation breakpoint
  JOURNAL   Hum Mol Genet 6 (9), 1491-1497 (1997)
   PUBMED   9285786
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF350881.2 and AL354795.17.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_017662.4.
            
            Summary: This gene is predominantly expressed in the kidney and
            colon, and encodes a protein containing an ion channel domain and a
            protein kinase domain. It is crucial for magnesium homeostasis, and
            plays an essential role in epithelial magnesium transport and in
            the active magnesium absorption in the gut and kidney. Mutations in
            this gene are associated with hypomagnesemia with secondary
            hypocalcemia. Alternatively spliced transcript variants encoding
            different isoforms have been noted for this gene. [provided by
            RefSeq, Apr 2010].
            
            Transcript Variant: This variant (a) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF350881.2, AY333282.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000360774.6/ ENSP00000354006.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6937              AF350881.2         174-7110
            6938-6939           AL354795.17        26183-26184         c
            6940-8252           AF350881.2         7111-8423
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q21.13"
     gene            1..8252
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /note="transient receptor potential cation channel
                     subfamily M member 6"
                     /db_xref="GeneID:140803"
                     /db_xref="HGNC:HGNC:17995"
                     /db_xref="MIM:607009"
     exon            1..98
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     CDS             66..6134
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /EC_number="2.7.11.1"
                     /note="isoform a is encoded by transcript variant a;
                     channel kinase 2; melastatin-related TRP cation channel 6"
                     /codon_start=1
                     /product="transient receptor potential cation channel
                     subfamily M member 6 isoform a"
                     /protein_id="NP_060132.3"
                     /db_xref="CCDS:CCDS6647.1"
                     /db_xref="GeneID:140803"
                     /db_xref="HGNC:HGNC:17995"
                     /db_xref="MIM:607009"
                     /translation="MKEQPVLERLQSQKSWIKGVFDKRECSTIIPSSKNPHRCTPVCQ
                     VCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQD
                     GEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVISVHGGIQNFTMPSKFKEIFS
                     QGLVKAAETTGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIENQRD
                     LIGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQ
                     KIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLA
                     DEGMLRPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDL
                     DLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALEQAMSDALVM
                     DRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQHTLLSGYRI
                     TLIDIGLVVEYLIGRAYRSNYTRKHFRALYNNLYRKYKHQRHSSGNRNESAESTLHSQ
                     FIRTAQPYKFKEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMA
                     MFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEK
                     AFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMR
                     KNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSSKESASVK
                     EYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVKFWFYTMAYLAFLMLF
                     TYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVREICISEPGKFTQKVKVWISEYWNLTE
                     TVAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAK
                     MTANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEI
                     DVCSSQPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNR
                     YRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKL
                     HDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVSFIKDSLLSL
                     DSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVL
                     GSMEIAGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQ
                     ETQSSIVVSGVSPNRQAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQD
                     IQTEVLVHLTGQTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTM
                     SSPLSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQKQA
                     QDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFHKEEKLMK
                     ICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQGLQVPIITVNACSQSDQLNP
                     EPGENSISEEEYSKNWFTVSKFSHTGVEPYIHQKMKTKEIGQCAIQISDYLKQSQEDL
                     SKNSLWNSRSTNLNRNSLLKSSIGVDKISASLKSPQEPHHHYSAIERNNLMRLSQTIP
                     FTPVQLFAGEEITVYRLEESSPLNLDKSMSSWSQRGRAAMIQVLSREEMDGGLRKAMR
                     VVSTWSEDDILKPGQVFIVKSFLPEVVRTWHKIFQESTVLHLCLREIQQQRAAQKLIY
                     TFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNGDEITPTNTL
                     EELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEVKQSRGMVFGPANLGEDA
                     IRNFIAKHHCNSCCRKLKLPDLKRNDYSPERINSTFGLEIKIESAEEPPARETGRNSP
                     EDDMQL"
     exon            99..178
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            179..217
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            218..395
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            396..609
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            610..734
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            735..906
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            907..1075
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1199
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1272
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1373
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1508
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1509..1562
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1563..1703
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1796
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1797..2074
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            2075..2303
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            2304..2456
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            2457..2603
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            2604..2732
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            2733..2984
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            2985..3159
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            3160..3274
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            3275..3468
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            3469..3601
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            3602..4737
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            4738..4850
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            4851..4971
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            4972..5063
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            5064..5122
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            5123..5148
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            5149..5199
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            5200..5265
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            5266..5552
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            5553..5635
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            5636..5841
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            5842..5893
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            5894..6000
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            6001..8252
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8230..8235
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /note="hexamer: AATACA"
     polyA_site      8252
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /note="major polyA site"
ORIGIN      
        1 agagtttctg cctccacagt taaaagctca gacagggctt tgggagcagg gcctgcaaat
       61 caaagatgaa agaacaacct gtcttggagc gcttgcagtc ccagaaatcc tggattaaag
      121 gagtatttga caagagagaa tgtagcacaa tcatacccag ctcaaaaaat cctcacagat
      181 gtactccagt atgccaagtc tgccagaatt taatcaggtg ttactgtggc cgactgattg
      241 gagaccatgc tgggatagat tattcctgga ccatctcagc tgccaagggt aaagaaagtg
      301 aacaatggtc tgttgaaaag cacacaacga aaagcccaac agatactttt ggcacgatta
      361 atttccaaga tggagagcac acccatcatg ccaagtatat tagaacttct tatgatacaa
      421 aactggatca tctgttacat ttaatgttga aagagtggaa aatggaactg cccaagcttg
      481 tgatctcagt ccatgggggc atccagaact ttactatgcc ctctaaattt aaagagattt
      541 tcagccaagg tttggttaaa gctgcagaga caacaggagc gtggataata actgaaggca
      601 tcaatacagg agtgtccaag catgttgggg atgccttgaa atcccattcc tctcattcct
      661 tgagaaaaat ctggacagtt ggaatccctc cttggggtgt cattgagaac cagagagacc
      721 ttattggaaa agatgtggtg tgcctgtacc agactctgga taaccccctc agcaagctca
      781 caacactcaa cagcatgcac tcgcacttca tcctgtctga tgatgggacc gtgggcaagt
      841 atggaaatga aatgaagctc agaaggaacc tggagaagta cctctctctg cagaaaatac
      901 actgccgctc aagacaaggc gtgccggtcg tggggctggt ggtggaaggc ggtcccaacg
      961 tcatcctgtc agtgtgggag actgtcaagg acaaggaccc agtggtggtg tgtgagggca
     1021 caggtagggc ggctgacctc ctggccttca cacacaaaca cctggcagat gaagggatgc
     1081 tgcgacctca ggtgaaagag gagatcatct gcatgattca gaacactttc aactttagtc
     1141 ttaaacagtc caagcacctt ttccaaattc taatggagtg tatggttcac agggattgta
     1201 ttaccatatt tgatgctgac tctgaagagc agcaagacct ggacttagca atcctaacag
     1261 ctttgctgaa gggcacaaat ttatcagcgt cagagcaatt aaatctggca atggcttggg
     1321 acagggtgga cattgccaag aaacatatcc taatttatga acaacactgg aagcctgatg
     1381 ccctggaaca agcaatgtca gatgctttag tgatggatcg ggtggatttt gtgaagctct
     1441 taatagaata tggagtgaac ctccatcgct ttcttaccat ccctcgactg gaagagctct
     1501 acaatacaaa acaaggacct actaatacac tcttgcatca tctcgtccaa gatgtgaaac
     1561 agcataccct tctttcaggc taccgaataa ccttgattga cattggatta gtagtagaat
     1621 acctcattgg tagagcatat cgcagcaact acactagaaa acatttcaga gccctctaca
     1681 acaacctcta cagaaaatac aagcaccaga gacactcctc aggaaataga aatgagtctg
     1741 cagaaagtac gctgcactcc cagttcatta gaactgcaca gccatacaaa ttcaaggaaa
     1801 agtctatagt ccttcataaa tcaaggaaga agtcaaaaga acaaaatgta tcagatgacc
     1861 ctgagtctac tggctttctt tacccttaca atgacctgct ggtttgggct gtgctgatga
     1921 aaaggcagaa gatggctatg ttcttctggc agcatggaga ggaggccacg gttaaagccg
     1981 tgattgcgtg tatcctctac cgggcaatgg cccatgaagc taaggagagt cacatggtgg
     2041 atgatgcctc agaagagttg aagaattact caaaacagtt tggccagctg gctctggact
     2101 tgttggagaa ggcattcaag cagaatgagc gcatggccat gacgctgttg acgtatgaac
     2161 tcaggaactg gagcaattcg acctgcctta aactggccgt gtcgggagga ttacgaccct
     2221 ttgtttcaca tacttgtacc cagatgctac tgacagacat gtggatgggg aggctgaaaa
     2281 tgaggaaaaa ctcttggtta aagattatta taagcattat tttaccaccc accattttga
     2341 cactggaatt taaaagcaaa gctgagatgt cacatgttcc ccagtcccag gacttccaat
     2401 ttatgtggta ttacagtgac cagaacgcca gcagttccaa agaaagtgct tctgtgaaag
     2461 agtatgattt ggaaaggggc catgatgaga aactggatga aaatcagcat tttggtttgg
     2521 aaagtgggca ccaacacctt ccgtggacca ggaaagtcta tgagttctac agtgctccaa
     2581 ttgtcaagtt ttggttttat acgatggcgt atttggcatt cctcatgctg ttcacttaca
     2641 ccgtgttggt ggagatgcag ccccagccca gcgtgcagga gtggcttgtt agcatttaca
     2701 tcttcaccaa tgctattgag gtggtcaggg agatctgtat ttcagaacct gggaagttta
     2761 cccaaaaggt gaaggtatgg attagtgagt actggaactt aacagaaact gtggccattg
     2821 gcctgttttc agctggcttc gtccttcgat ggggtgaccc tccttttcac acagcgggaa
     2881 gactgatcta ctgcatagac atcatattct ggttctcacg gctcctggac ttctttgctg
     2941 tgaatcaaca tgcaggtcca tatgtgacca tgattgcaaa aatgacagca aacatgttct
     3001 atattgtgat catcatggcc atagtcctgc tgagctttgg agtggcacgc aaggccatcc
     3061 tttcgccaaa agagccacca tcttggagtc tagctcgaga tattgtattt gagccatact
     3121 ggatgatata cggagaagtc tatgctggag aaatagatgt ttgttcaagc cagccatcct
     3181 gccctcctgg ttcttttctt actccattct tgcaagctgt ctacctcttc gtgcaatata
     3241 tcatcatggt gaacctgttg attgctttct tcaacaacgt ttacttagat atggaatcca
     3301 tttcaaataa cctgtggaaa tacaaccgct atcgctacat catgacctac cacgagaagc
     3361 cctggctgcc cccacctctc atcctgctga gccacgtggg ccttctcctc cgccgcctgt
     3421 gctgtcatcg agctcctcac gaccaagaag agggtgacgt tggattaaaa ctctacctca
     3481 gtaaggagga tctgaaaaaa cttcatgatt ttgaggagca gtgcgtggaa aaatacttcc
     3541 atgagaagat ggaagatgtg aattgtagtt gtgaggaacg aatccgagtg acatcagaaa
     3601 gggttacaga gatgtacttc cagctgaaag aaatgaatga aaaggtgtct tttataaagg
     3661 actccttact gtctttggac agccaggtgg gacacctgca ggatctctct gccctgactg
     3721 tggataccct gaaagtcctt tctgctgttg acactttgca agaggatgag gctctcctgg
     3781 ccaagagaaa gcattctact tgcaaaaaac ttccccacag ctggagcaat gtcatctgtg
     3841 cagaggttct aggcagcatg gagatcgctg gagagaagaa ataccagtat tatagcatgc
     3901 cctcttcttt gctgaggagc ctggctggag gccggcatcc cccaagagtg cagagggggg
     3961 cacttcttga gattacaaac agtaaaagag aggctacaaa tgtaagaaat gaccaggaaa
     4021 ggcaagaaac acaaagtagt atagtggttt ctggggtgtc tcctaacagg caagcacact
     4081 caaagtatgg ccagtttctt ctggtcccct ctaatctaaa gcgagttcct ttttcagcag
     4141 aaactgtctt gcctctgtcc agaccctctg tgccagatgt gctggcaact gaacaggaca
     4201 tccagactga ggttcttgtt catctgactg ggcagacccc agttgtctct gactgggcat
     4261 cagtggatga acccaaggaa aagcacgagc ctattgctca cttactggat ggacaagaca
     4321 aggcagagca agtgctaccc actttgagtt gcacacctga acccatgaca atgagctccc
     4381 ctctttccca agccaagatc atgcaaactg gaggtggata tgtaaactgg gcattttcag
     4441 aaggtgatga aactggtgtg tttagcatca agaaaaagtg gcaaacctgc ttgccctcca
     4501 cttgtgacag tgattcctct cggagtgaac agcaccagaa gcaggcccag gacagctccc
     4561 tatctgataa ctcaacaaga tcggcccaga gtagtgaatg ctcagaggtg ggaccatggc
     4621 ttcagccaaa cacatccttt tggatcaatc ctctccgcag atacaggccc ttcgctagga
     4681 gtcatagttt tagattccat aaggaggaga aattgatgaa gatctgtaag attaaaaatc
     4741 tttcaggctc ttcagaaata gggcagggag catgggtcaa agcgaaaatg ctaaccaaag
     4801 acaggagact gtcaaagaaa aagaagaata ctcaaggact ccaggtgcca atcataacag
     4861 tcaatgcctg ctctcagagt gaccagttga atccagagcc aggagaaaac agcatctctg
     4921 aagaggagta cagcaagaac tggttcacag tgtccaaatt tagtcacaca ggtgtagaac
     4981 cttacataca tcagaaaatg aaaactaaag aaattggaca atgtgctata caaatcagtg
     5041 attacctaaa gcagtctcaa gaggatctca gcaaaaactc tttgtggaat tccaggagca
     5101 ccaacctcaa taggaactcc ctgctgaaaa gttcaattgg agttgacaag atctcagcct
     5161 ccttaaaaag ccctcaagag cctcaccatc attattcagc cattgaaagg aataatttaa
     5221 tgaggctttc tcagaccata ccatttacac cagtccaact gtttgcagga gaagaaataa
     5281 ctgtctacag gttggaggag agttcccctt taaaccttga taaaagcatg tcctcttggt
     5341 ctcagcgtgg gagagcggca atgatccagg tattgtcccg agaggagatg gatgggggcc
     5401 tccgtaaagc tatgagagtc gtcagcactt ggtctgagga tgacattctc aagccgggac
     5461 aagttttcat tgtcaagtcc tttcttcctg aggttgtgcg gacatggcat aaaatcttcc
     5521 aggagagcac tgtgcttcat ctttgcctca gggaaattca acaacaaaga gctgctcaaa
     5581 aattgatcta taccttcaac caagtgaaac cacaaaccat accctacaca ccaaggttcc
     5641 tggaagtttt cttaatctac tgccattcag ccaaccagtg gttgaccatt gagaagtata
     5701 tgacagggga gttccggaag tataacaaca acaatggtga tgaaatcacc cccaccaaca
     5761 ccctggagga gctgatgttg gctttctctc actggaccta tgagtacact cggggagagc
     5821 tgctggtttt agatttgcaa ggtgttggag aaaatttgac agatccatct gttataaaac
     5881 ctgaagtcaa acaatcaaga ggaatggtgt ttggaccggc caatttgggg gaagatgcaa
     5941 ttagaaactt cattgcaaaa catcattgta actcctgctg ccggaagctc aaactcccgg
     6001 atttaaaaag aaatgactat tcccctgaaa ggataaattc cacctttgga cttgagataa
     6061 aaatagaatc agctgaggag cctccagcaa gggagacggg tagaaattcc ccagaagatg
     6121 atatgcaact ataaaaaggg aggagcaaga agatcccagt gcttgccctg cctgccagga
     6181 actctgtgat aacatagatt gatcaacgtg atgttgatta catcagcgtc tccttgggac
     6241 acgccttctg agcctcacat ctccttctgt tcaaaggcct cattggtata tgatcaatgg
     6301 gttctcctag acactgacct ctgtccaggg cactttgcag ctccatcctc aagttccaca
     6361 cgaagatgct tggatgagtc agctgggaat attgttcttg tgtacctcat tgctttagct
     6421 ggtcacttgg aactttggag cagaatcctg cacattaaag gatggggttg ggggggatac
     6481 atttatttta ttttctcact atgtatgcag actggacccc ctactactat ttgtcacctc
     6541 acccacagat tgtatttatg tctatatata tgttcataaa aagttatgtg atttcctcct
     6601 ctgtcttttc cacaacatag gactttgaat agcaatgata ggaaaaacaa tggaacaagg
     6661 gtgggtttgc acagattgga gcacattcct gcacaaacta ccaagtatac tggtgaaatc
     6721 tcgatgggtt tcagatattg tcagtgaatc atatgatgcc tggatatttc aggtttctgt
     6781 aaaagaaagg gaaacctaaa acaaataccc ttccatatat aatatatatg gaatatgtat
     6841 attatatata tttttatata tataatatat atggaatata tatattatat atataaaata
     6901 catatggaat atatatattt tatatatata tatatatatt tttatttttg agatggagtt
     6961 tcactctttt tacccaggct ggagtgcaat gatgcgatct cactgcaacc tctgcctccc
     7021 gggcttgagc gattcttgtg tctcagtctt ccgggtagct gggactacag gtgtgcacca
     7081 ctatgcctgg ctaattttgt atttttagta gagatggggt ttcaccatgt tggccaggct
     7141 ggtctcaaac tcctgacctc agatgatcca cctgccttgg cttcccaaag tgctgggatt
     7201 acaggcgtga gccactgcgc ctggcctttt tttttttttt tttaaacgag aacaagaata
     7261 tgaagaactg gaaatcatta agaaagggtt tcccttcctt aaagctcagg ggtactatta
     7321 gttaggagtt gactaactca acctgtaaaa caccactcct ccttccaaag ttgtatatat
     7381 aatattgcag gttaaattac tttatgtcag gtcctatgaa gaaagatacg gtttcagact
     7441 gaaaacatgt ttcacaggtg tttgcttcct tccagagcag agttccctat tcccctggca
     7501 taaagaatgt atatatattt tgaaatatgg ctgagaacat gtcattggtt tgtgaggcct
     7561 aaggtgaagc actcctggca gccacactgt gtagtgtatt tgagggatca gtcatccctc
     7621 ttgtatgctg ggcctggttg ccctacctcg aacaagcacc agcttttcac acaaggagag
     7681 atgtggggct gggagtcctc tccccatcct attgcatctc ctttcttatt ataagctgtt
     7741 ccagttcaca ggcagcaaac ctcctgggtt tgaaaaattc caacttattt ttatctttaa
     7801 tcctgacatt agctgacttg ctagtgagct tgctttaaaa atctacactc ttgcattctt
     7861 aggcatacag gggaaatgtt gaaaaggaag gtggaaaacc aagaatttag tttgccaatg
     7921 attgcctctg attcttgtaa gtttgagttc cacaagggct aatttattcc ccttttactt
     7981 gggttttggg gtggtggaaa gcgggaaatt tgggtgattt gttgattggc aatgaggata
     8041 aaatgttaat acttttttgg ggacttaaca actttatcct attctacaag tcagtaaagg
     8101 aacaattggt actcacctca gtgctgcact caactatgga aagaggcaga gtttgcttgc
     8161 ccaattgcca aactaaagac atcagttcat tggtcaaata tttgttacct ggaatggaac
     8221 ttgaaagcaa atacatttgg atttcaaatt tc
//
