LOCUS       NM_001177310            8244 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens transient receptor potential cation channel subfamily
            M member 6 (TRPM6), transcript variant b, mRNA.
ACCESSION   NM_001177310
VERSION     NM_001177310.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8244)
  AUTHORS   Huang,S., Ge,Y., Li,Y., Cui,N., Tan,L., Guo,S., Wang,S., Hao,L.,
            Lei,G. and Yang,X.
  TITLE     Magnesium Status, Genetic Variants of Magnesium-Related Ion Channel
            Transient Receptor Potential Membrane Melastatin 6 (TRPM6) and the
            Risk of Gestational Diabetes Mellitus in Chinese Pregnant Women: A
            Nested Case-Control Study
  JOURNAL   Mol Nutr Food Res 67 (22), e2200835 (2023)
   PUBMED   37759402
  REMARK    GeneRIF: Magnesium Status, Genetic Variants of Magnesium-Related
            Ion Channel Transient Receptor Potential Membrane Melastatin 6
            (TRPM6) and the Risk of Gestational Diabetes Mellitus in Chinese
            Pregnant Women: A Nested Case-Control Study.
REFERENCE   2  (bases 1 to 8244)
  AUTHORS   Kim,E.Y. and Lee,J.M.
  TITLE     Transcriptional Control of Trpm6 by the Nuclear Receptor FXR
  JOURNAL   Int J Mol Sci 23 (4), 1980 (2022)
   PUBMED   35216094
  REMARK    GeneRIF: Transcriptional Control of Trpm6 by the Nuclear Receptor
            FXR.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8244)
  AUTHORS   Andriule,I., Pangonyte,D., Almanaityte,M., Patamsyte,V.,
            Kupryte,M., Karciauskas,D., Mubagwa,K. and Macianskiene,R.
  TITLE     Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes
            from all four chamber walls of the human heart
  JOURNAL   Sci Rep 11 (1), 15445 (2021)
   PUBMED   34326388
  REMARK    GeneRIF: Evidence for the expression of TRPM6 and TRPM7 in
            cardiomyocytes from all four chamber walls of the human heart.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8244)
  AUTHORS   Zouharova,M., Herman,P., Hofbauerova,K., Vondrasek,J. and
            Bousova,K.
  TITLE     TRPM6 N-Terminal CaM- and S100A1-Binding Domains
  JOURNAL   Int J Mol Sci 20 (18), 4430 (2019)
   PUBMED   31505788
  REMARK    GeneRIF: data unambiguously confirm that CaM and S100A1 share the
            same binding domain at the TRPM6 N-terminus although the
            ligand-binding mechanism is different.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8244)
  AUTHORS   Tan,J., Yan,T., Li,Z., Huang,J. and Cai,R.
  TITLE     [Analysis of TRPM6 gene variant in a pedigree affected with
            hypocalcemia secondary to hypomagnesemia]
  JOURNAL   Zhonghua Yi Xue Yi Chuan Xue Za Zhi 36 (8), 805-808 (2019)
   PUBMED   31400133
  REMARK    GeneRIF: The homozygous c.3311C>T (p.Pro1104Leu) variant of the
            TRPM6 gene probably underlies hypocalcemia secondary to
            hypomagnesemia in this pedigree.
REFERENCE   6  (bases 1 to 8244)
  AUTHORS   Walder,R.Y., Landau,D., Meyer,P., Shalev,H., Tsolia,M.,
            Borochowitz,Z., Boettger,M.B., Beck,G.E., Englehardt,R.K., Carmi,R.
            and Sheffield,V.C.
  TITLE     Mutation of TRPM6 causes familial hypomagnesemia with secondary
            hypocalcemia
  JOURNAL   Nat Genet 31 (2), 171-174 (2002)
   PUBMED   12032570
  REMARK    GeneRIF: Individuals with TRPM6 mutations have abnormal renal
            magnesium excretion. Deficiency causes hypomagnesemia with
            secondary hypocalcemia.
REFERENCE   7  (bases 1 to 8244)
  AUTHORS   Schlingmann,K.P., Weber,S., Peters,M., Niemann Nejsum,L.,
            Vitzthum,H., Klingel,K., Kratz,M., Haddad,E., Ristoff,E.,
            Dinour,D., Syrrou,M., Nielsen,S., Sassen,M., Waldegger,S.,
            Seyberth,H.W. and Konrad,M.
  TITLE     Hypomagnesemia with secondary hypocalcemia is caused by mutations
            in TRPM6, a new member of the TRPM gene family
  JOURNAL   Nat Genet 31 (2), 166-170 (2002)
   PUBMED   12032568
  REMARK    GeneRIF: Deficiency /mutations of TRPM6 causes hypomagnesemia with
            secondary hypocalcemia
REFERENCE   8  (bases 1 to 8244)
  AUTHORS   Riazanova,L.V., Pavur,K.S., Petrov,A.N., Dorovkov,M.V. and
            Riazanov,A.G.
  TITLE     [Novel type of signaling molecules: protein kinases covalently
            linked to ion channels]
  JOURNAL   Mol Biol (Mosk) 35 (2), 321-332 (2001)
   PUBMED   11357414
REFERENCE   9  (bases 1 to 8244)
  AUTHORS   Ryazanov,A.G., Pavur,K.S. and Dorovkov,M.V.
  TITLE     Alpha-kinases: a new class of protein kinases with a novel
            catalytic domain
  JOURNAL   Curr Biol 9 (2), R43-R45 (1999)
   PUBMED   10021370
REFERENCE   10 (bases 1 to 8244)
  AUTHORS   Walder,R.Y., Shalev,H., Brennan,T.M., Carmi,R., Elbedour,K.,
            Scott,D.A., Hanauer,A., Mark,A.L., Patil,S., Stone,E.M. and
            Sheffield,V.C.
  TITLE     Familial hypomagnesemia maps to chromosome 9q, not to the X
            chromosome: genetic linkage mapping and analysis of a balanced
            translocation breakpoint
  JOURNAL   Hum Mol Genet 6 (9), 1491-1497 (1997)
   PUBMED   9285786
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY333283.1, AF350881.2 and
            AL354795.17.
            
            On Aug 13, 2020 this sequence version replaced NM_001177310.1.
            
            Summary: This gene is predominantly expressed in the kidney and
            colon, and encodes a protein containing an ion channel domain and a
            protein kinase domain. It is crucial for magnesium homeostasis, and
            plays an essential role in epithelial magnesium transport and in
            the active magnesium absorption in the gut and kidney. Mutations in
            this gene are associated with hypomagnesemia with secondary
            hypocalcemia. Alternatively spliced transcript variants encoding
            different isoforms have been noted for this gene. [provided by
            RefSeq, Apr 2010].
            
            Transcript Variant: This variant (b) contains an alternate 5'
            terminal exon compared to variant a. This results in a shorter
            isoform (b) with a distinct N-terminus compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY333283.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6517              AY333283.1         1-6517
            6518-6929           AF350881.2         6699-7110
            6930-6931           AL354795.17        26183-26184         c
            6932-8244           AF350881.2         7111-8423
FEATURES             Location/Qualifiers
     source          1..8244
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q21.13"
     gene            1..8244
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /note="transient receptor potential cation channel
                     subfamily M member 6"
                     /db_xref="GeneID:140803"
                     /db_xref="HGNC:HGNC:17995"
                     /db_xref="MIM:607009"
     exon            1..90
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..6126
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /EC_number="2.7.11.1"
                     /note="isoform b is encoded by transcript variant b;
                     channel kinase 2; melastatin-related TRP cation channel 6"
                     /codon_start=1
                     /product="transient receptor potential cation channel
                     subfamily M member 6 isoform b"
                     /protein_id="NP_001170781.1"
                     /db_xref="CCDS:CCDS55319.1"
                     /db_xref="GeneID:140803"
                     /db_xref="HGNC:HGNC:17995"
                     /db_xref="MIM:607009"
                     /translation="MIILSKSQKSWIKGVFDKRECSTIIPSSKNPHRCTPVCQVCQNL
                     IRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHTH
                     HAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVISVHGGIQNFTMPSKFKEIFSQGLVK
                     AAETTGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIENQRDLIGKD
                     VVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCR
                     SRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGML
                     RPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDLDLAIL
                     TALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDF
                     VKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQHTLLSGYRITLIDI
                     GLVVEYLIGRAYRSNYTRKHFRALYNNLYRKYKHQRHSSGNRNESAESTLHSQFIRTA
                     QPYKFKEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQ
                     HGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQN
                     ERMAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWL
                     KIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSSKESASVKEYDLE
                     RGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVKFWFYTMAYLAFLMLFTYTVL
                     VEMQPQPSVQEWLVSIYIFTNAIEVVREICISEPGKFTQKVKVWISEYWNLTETVAIG
                     LFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANM
                     FYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSS
                     QPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIM
                     TYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKLHDFEE
                     QCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVSFIKDSLLSLDSQVG
                     HLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVLGSMEI
                     AGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQETQSS
                     IVVSGVSPNRQAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEV
                     LVHLTGQTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLS
                     QAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQKQAQDSSL
                     SDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFHKEEKLMKICKIK
                     NLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQGLQVPIITVNACSQSDQLNPEPGEN
                     SISEEEYSKNWFTVSKFSHTGVEPYIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSL
                     WNSRSTNLNRNSLLKSSIGVDKISASLKSPQEPHHHYSAIERNNLMRLSQTIPFTPVQ
                     LFAGEEITVYRLEESSPLNLDKSMSSWSQRGRAAMIQVLSREEMDGGLRKAMRVVSTW
                     SEDDILKPGQVFIVKSFLPEVVRTWHKIFQESTVLHLCLREIQQQRAAQKLIYTFNQV
                     KPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNGDEITPTNTLEELML
                     AFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEVKQSRGMVFGPANLGEDAIRNFI
                     AKHHCNSCCRKLKLPDLKRNDYSPERINSTFGLEIKIESAEEPPARETGRNSPEDDMQ
                     L"
     exon            91..170
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            171..209
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            210..387
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            388..601
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            602..726
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            727..898
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            899..1067
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1191
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1192..1264
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1365
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1366..1500
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1501..1554
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1555..1695
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..1788
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            1789..2066
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            2067..2295
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            2296..2448
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            2449..2595
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            2596..2724
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            2725..2976
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            2977..3151
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            3152..3266
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            3267..3460
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            3461..3593
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            3594..4729
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            4730..4842
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            4843..4963
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            4964..5055
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            5056..5114
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            5115..5140
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            5141..5191
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            5192..5257
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            5258..5544
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            5545..5627
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            5628..5833
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            5834..5885
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            5886..5992
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     exon            5993..8244
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8222..8227
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /note="hexamer: AATACA"
     polyA_site      8244
                     /gene="TRPM6"
                     /gene_synonym="CHAK2; HMGX; HOMG; HOMG1; HSH"
                     /note="major polyA site"
ORIGIN      
        1 atctaacgag gatctagaat agctaggatt tatgaaatac cggctttcaa gttctctggt
       61 ctccacccaa agatgattat cctatctaag tcccagaaat cctggattaa aggagtattt
      121 gacaagagag aatgtagcac aatcataccc agctcaaaaa atcctcacag atgtactcca
      181 gtatgccaag tctgccagaa tttaatcagg tgttactgtg gccgactgat tggagaccat
      241 gctgggatag attattcctg gaccatctca gctgccaagg gtaaagaaag tgaacaatgg
      301 tctgttgaaa agcacacaac gaaaagccca acagatactt ttggcacgat taatttccaa
      361 gatggagagc acacccatca tgccaagtat attagaactt cttatgatac aaaactggat
      421 catctgttac atttaatgtt gaaagagtgg aaaatggaac tgcccaagct tgtgatctca
      481 gtccatgggg gcatccagaa ctttactatg ccctctaaat ttaaagagat tttcagccaa
      541 ggtttggtta aagctgcaga gacaacagga gcgtggataa taactgaagg catcaataca
      601 ggagtgtcca agcatgttgg ggatgccttg aaatcccatt cctctcattc cttgagaaaa
      661 atctggacag ttggaatccc tccttggggt gtcattgaga accagagaga ccttattgga
      721 aaagatgtgg tgtgcctgta ccagactctg gataaccccc tcagcaagct cacaacactc
      781 aacagcatgc actcgcactt catcctgtct gatgatggga ccgtgggcaa gtatggaaat
      841 gaaatgaagc tcagaaggaa cctggagaag tacctctctc tgcagaaaat acactgccgc
      901 tcaagacaag gcgtgccggt cgtggggctg gtggtggaag gcggtcccaa cgtcatcctg
      961 tcagtgtggg agactgtcaa ggacaaggac ccagtggtgg tgtgtgaggg cacaggtagg
     1021 gcggctgacc tcctggcctt cacacacaaa cacctggcag atgaagggat gctgcgacct
     1081 caggtgaaag aggagatcat ctgcatgatt cagaacactt tcaactttag tcttaaacag
     1141 tccaagcacc ttttccaaat tctaatggag tgtatggttc acagggattg tattaccata
     1201 tttgatgctg actctgaaga gcagcaagac ctggacttag caatcctaac agctttgctg
     1261 aagggcacaa atttatcagc gtcagagcaa ttaaatctgg caatggcttg ggacagggtg
     1321 gacattgcca agaaacatat cctaatttat gaacaacact ggaagcctga tgccctggaa
     1381 caagcaatgt cagatgcttt agtgatggat cgggtggatt ttgtgaagct cttaatagaa
     1441 tatggagtga acctccatcg ctttcttacc atccctcgac tggaagagct ctacaataca
     1501 aaacaaggac ctactaatac actcttgcat catctcgtcc aagatgtgaa acagcatacc
     1561 cttctttcag gctaccgaat aaccttgatt gacattggat tagtagtaga atacctcatt
     1621 ggtagagcat atcgcagcaa ctacactaga aaacatttca gagccctcta caacaacctc
     1681 tacagaaaat acaagcacca gagacactcc tcaggaaata gaaatgagtc tgcagaaagt
     1741 acgctgcact cccagttcat tagaactgca cagccataca aattcaagga aaagtctata
     1801 gtccttcata aatcaaggaa gaagtcaaaa gaacaaaatg tatcagatga ccctgagtct
     1861 actggctttc tttaccctta caatgacctg ctggtttggg ctgtgctgat gaaaaggcag
     1921 aagatggcta tgttcttctg gcagcatgga gaggaggcca cggttaaagc cgtgattgcg
     1981 tgtatcctct accgggcaat ggcccatgaa gctaaggaga gtcacatggt ggatgatgcc
     2041 tcagaagagt tgaagaatta ctcaaaacag tttggccagc tggctctgga cttgttggag
     2101 aaggcattca agcagaatga gcgcatggcc atgacgctgt tgacgtatga actcaggaac
     2161 tggagcaatt cgacctgcct taaactggcc gtgtcgggag gattacgacc ctttgtttca
     2221 catacttgta cccagatgct actgacagac atgtggatgg ggaggctgaa aatgaggaaa
     2281 aactcttggt taaagattat tataagcatt attttaccac ccaccatttt gacactggaa
     2341 tttaaaagca aagctgagat gtcacatgtt ccccagtccc aggacttcca atttatgtgg
     2401 tattacagtg accagaacgc cagcagttcc aaagaaagtg cttctgtgaa agagtatgat
     2461 ttggaaaggg gccatgatga gaaactggat gaaaatcagc attttggttt ggaaagtggg
     2521 caccaacacc ttccgtggac caggaaagtc tatgagttct acagtgctcc aattgtcaag
     2581 ttttggtttt atacgatggc gtatttggca ttcctcatgc tgttcactta caccgtgttg
     2641 gtggagatgc agccccagcc cagcgtgcag gagtggcttg ttagcattta catcttcacc
     2701 aatgctattg aggtggtcag ggagatctgt atttcagaac ctgggaagtt tacccaaaag
     2761 gtgaaggtat ggattagtga gtactggaac ttaacagaaa ctgtggccat tggcctgttt
     2821 tcagctggct tcgtccttcg atggggtgac cctccttttc acacagcggg aagactgatc
     2881 tactgcatag acatcatatt ctggttctca cggctcctgg acttctttgc tgtgaatcaa
     2941 catgcaggtc catatgtgac catgattgca aaaatgacag caaacatgtt ctatattgtg
     3001 atcatcatgg ccatagtcct gctgagcttt ggagtggcac gcaaggccat cctttcgcca
     3061 aaagagccac catcttggag tctagctcga gatattgtat ttgagccata ctggatgata
     3121 tacggagaag tctatgctgg agaaatagat gtttgttcaa gccagccatc ctgccctcct
     3181 ggttcttttc ttactccatt cttgcaagct gtctacctct tcgtgcaata tatcatcatg
     3241 gtgaacctgt tgattgcttt cttcaacaac gtttacttag atatggaatc catttcaaat
     3301 aacctgtgga aatacaaccg ctatcgctac atcatgacct accacgagaa gccctggctg
     3361 cccccacctc tcatcctgct gagccacgtg ggccttctcc tccgccgcct gtgctgtcat
     3421 cgagctcctc acgaccaaga agagggtgac gttggattaa aactctacct cagtaaggag
     3481 gatctgaaaa aacttcatga ttttgaggag cagtgcgtgg aaaaatactt ccatgagaag
     3541 atggaagatg tgaattgtag ttgtgaggaa cgaatccgag tgacatcaga aagggttaca
     3601 gagatgtact tccagctgaa agaaatgaat gaaaaggtgt cttttataaa ggactcctta
     3661 ctgtctttgg acagccaggt gggacacctg caggatctct ctgccctgac tgtggatacc
     3721 ctgaaagtcc tttctgctgt tgacactttg caagaggatg aggctctcct ggccaagaga
     3781 aagcattcta cttgcaaaaa acttccccac agctggagca atgtcatctg tgcagaggtt
     3841 ctaggcagca tggagatcgc tggagagaag aaataccagt attatagcat gccctcttct
     3901 ttgctgagga gcctggctgg aggccggcat cccccaagag tgcagagggg ggcacttctt
     3961 gagattacaa acagtaaaag agaggctaca aatgtaagaa atgaccagga aaggcaagaa
     4021 acacaaagta gtatagtggt ttctggggtg tctcctaaca ggcaagcaca ctcaaagtat
     4081 ggccagtttc ttctggtccc ctctaatcta aagcgagttc ctttttcagc agaaactgtc
     4141 ttgcctctgt ccagaccctc tgtgccagat gtgctggcaa ctgaacagga catccagact
     4201 gaggttcttg ttcatctgac tgggcagacc ccagttgtct ctgactgggc atcagtggat
     4261 gaacccaagg aaaagcacga gcctattgct cacttactgg atggacaaga caaggcagag
     4321 caagtgctac ccactttgag ttgcacacct gaacccatga caatgagctc ccctctttcc
     4381 caagccaaga tcatgcaaac tggaggtgga tatgtaaact gggcattttc agaaggtgat
     4441 gaaactggtg tgtttagcat caagaaaaag tggcaaacct gcttgccctc cacttgtgac
     4501 agtgattcct ctcggagtga acagcaccag aagcaggccc aggacagctc cctatctgat
     4561 aactcaacaa gatcggccca gagtagtgaa tgctcagagg tgggaccatg gcttcagcca
     4621 aacacatcct tttggatcaa tcctctccgc agatacaggc ccttcgctag gagtcatagt
     4681 tttagattcc ataaggagga gaaattgatg aagatctgta agattaaaaa tctttcaggc
     4741 tcttcagaaa tagggcaggg agcatgggtc aaagcgaaaa tgctaaccaa agacaggaga
     4801 ctgtcaaaga aaaagaagaa tactcaagga ctccaggtgc caatcataac agtcaatgcc
     4861 tgctctcaga gtgaccagtt gaatccagag ccaggagaaa acagcatctc tgaagaggag
     4921 tacagcaaga actggttcac agtgtccaaa tttagtcaca caggtgtaga accttacata
     4981 catcagaaaa tgaaaactaa agaaattgga caatgtgcta tacaaatcag tgattaccta
     5041 aagcagtctc aagaggatct cagcaaaaac tctttgtgga attccaggag caccaacctc
     5101 aataggaact ccctgctgaa aagttcaatt ggagttgaca agatctcagc ctccttaaaa
     5161 agccctcaag agcctcacca tcattattca gccattgaaa ggaataattt aatgaggctt
     5221 tctcagacca taccatttac accagtccaa ctgtttgcag gagaagaaat aactgtctac
     5281 aggttggagg agagttcccc tttaaacctt gataaaagca tgtcctcttg gtctcagcgt
     5341 gggagagcgg caatgatcca ggtattgtcc cgagaggaga tggatggggg cctccgtaaa
     5401 gctatgagag tcgtcagcac ttggtctgag gatgacattc tcaagccggg acaagttttc
     5461 attgtcaagt cctttcttcc tgaggttgtg cggacatggc ataaaatctt ccaggagagc
     5521 actgtgcttc atctttgcct cagggaaatt caacaacaaa gagctgctca aaaattgatc
     5581 tataccttca accaagtgaa accacaaacc ataccctaca caccaaggtt cctggaagtt
     5641 ttcttaatct actgccattc agccaaccag tggttgacca ttgagaagta tatgacaggg
     5701 gagttccgga agtataacaa caacaatggt gatgaaatca cccccaccaa caccctggag
     5761 gagctgatgt tggctttctc tcactggacc tatgagtaca ctcggggaga gctgctggtt
     5821 ttagatttgc aaggtgttgg agaaaatttg acagatccat ctgttataaa acctgaagtc
     5881 aaacaatcaa gaggaatggt gtttggaccg gccaatttgg gggaagatgc aattagaaac
     5941 ttcattgcaa aacatcattg taactcctgc tgccggaagc tcaaactccc ggatttaaaa
     6001 agaaatgact attcccctga aaggataaat tccacctttg gacttgagat aaaaatagaa
     6061 tcagctgagg agcctccagc aagggagacg ggtagaaatt ccccagaaga tgatatgcaa
     6121 ctataaaaag ggaggagcaa gaagatccca gtgcttgccc tgcctgccag gaactctgtg
     6181 ataacataga ttgatcaacg tgatgttgat tacatcagcg tctccttggg acacgccttc
     6241 tgagcctcac atctccttct gttcaaaggc ctcattggta tatgatcaat gggttctcct
     6301 agacactgac ctctgtccag ggcactttgc agctccatcc tcaagttcca cacgaagatg
     6361 cttggatgag tcagctggga atattgttct tgtgtacctc attgctttag ctggtcactt
     6421 ggaactttgg agcagaatcc tgcacattaa aggatggggt tgggggggat acatttattt
     6481 tattttctca ctatgtatgc agactggacc ccctactact atttgtcacc tcacccacag
     6541 attgtattta tgtctatata tatgttcata aaaagttatg tgatttcctc ctctgtcttt
     6601 tccacaacat aggactttga atagcaatga taggaaaaac aatggaacaa gggtgggttt
     6661 gcacagattg gagcacattc ctgcacaaac taccaagtat actggtgaaa tctcgatggg
     6721 tttcagatat tgtcagtgaa tcatatgatg cctggatatt tcaggtttct gtaaaagaaa
     6781 gggaaaccta aaacaaatac ccttccatat ataatatata tggaatatgt atattatata
     6841 tatttttata tatataatat atatggaata tatatattat atatataaaa tacatatgga
     6901 atatatatat tttatatata tatatatata tttttatttt tgagatggag tttcactctt
     6961 tttacccagg ctggagtgca atgatgcgat ctcactgcaa cctctgcctc ccgggcttga
     7021 gcgattcttg tgtctcagtc ttccgggtag ctgggactac aggtgtgcac cactatgcct
     7081 ggctaatttt gtatttttag tagagatggg gtttcaccat gttggccagg ctggtctcaa
     7141 actcctgacc tcagatgatc cacctgcctt ggcttcccaa agtgctggga ttacaggcgt
     7201 gagccactgc gcctggcctt tttttttttt tttttaaacg agaacaagaa tatgaagaac
     7261 tggaaatcat taagaaaggg tttcccttcc ttaaagctca ggggtactat tagttaggag
     7321 ttgactaact caacctgtaa aacaccactc ctccttccaa agttgtatat ataatattgc
     7381 aggttaaatt actttatgtc aggtcctatg aagaaagata cggtttcaga ctgaaaacat
     7441 gtttcacagg tgtttgcttc cttccagagc agagttccct attcccctgg cataaagaat
     7501 gtatatatat tttgaaatat ggctgagaac atgtcattgg tttgtgaggc ctaaggtgaa
     7561 gcactcctgg cagccacact gtgtagtgta tttgagggat cagtcatccc tcttgtatgc
     7621 tgggcctggt tgccctacct cgaacaagca ccagcttttc acacaaggag agatgtgggg
     7681 ctgggagtcc tctccccatc ctattgcatc tcctttctta ttataagctg ttccagttca
     7741 caggcagcaa acctcctggg tttgaaaaat tccaacttat ttttatcttt aatcctgaca
     7801 ttagctgact tgctagtgag cttgctttaa aaatctacac tcttgcattc ttaggcatac
     7861 aggggaaatg ttgaaaagga aggtggaaaa ccaagaattt agtttgccaa tgattgcctc
     7921 tgattcttgt aagtttgagt tccacaaggg ctaatttatt ccccttttac ttgggttttg
     7981 gggtggtgga aagcgggaaa tttgggtgat ttgttgattg gcaatgagga taaaatgtta
     8041 atactttttt ggggacttaa caactttatc ctattctaca agtcagtaaa ggaacaattg
     8101 gtactcacct cagtgctgca ctcaactatg gaaagaggca gagtttgctt gcccaattgc
     8161 caaactaaag acatcagttc attggtcaaa tatttgttac ctggaatgga acttgaaagc
     8221 aaatacattt ggatttcaaa tttc
//
