LOCUS       NM_017636               4058 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens transient receptor potential cation channel subfamily
            M member 4 (TRPM4), transcript variant 1, mRNA.
ACCESSION   NM_017636
VERSION     NM_017636.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Pironet,A., Vandewiele,F. and Vennekens,R.
  TITLE     Exploring the role of TRPM4 in calcium-dependent triggered activity
            and cardiac arrhythmias
  JOURNAL   J Physiol 602 (8), 1605-1621 (2024)
   PUBMED   37128952
  REMARK    GeneRIF: Exploring the role of TRPM4 in calcium-dependent triggered
            activity and cardiac arrhythmias.
            Review article
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Ye,F., Liu,D. and Zhang,J.
  TITLE     Transient receptor potential channel TRPM4 favors oxidized
            low-density lipoprotein-induced coronary endothelial cell
            dysfunction via a mechanism involving ferroptosis
  JOURNAL   Tissue Cell 86, 102290 (2024)
   PUBMED   38103473
  REMARK    GeneRIF: Transient receptor potential channel TRPM4 favors oxidized
            low-density lipoprotein-induced coronary endothelial cell
            dysfunction via a mechanism involving ferroptosis.
REFERENCE   3  (bases 1 to 4058)
  AUTHORS   Hu,Y., Cang,J., Hiraishi,K., Fujita,T. and Inoue,R.
  TITLE     The Role of TRPM4 in Cardiac Electrophysiology and Arrhythmogenesis
  JOURNAL   Int J Mol Sci 24 (14), 11798 (2023)
   PUBMED   37511555
  REMARK    GeneRIF: The Role of TRPM4 in Cardiac Electrophysiology and
            Arrhythmogenesis.
            Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4058)
  AUTHORS   Krocker,J.D., Cotton,M.E., Schriner,J.B., Osborn,B.K.,
            Talanker,M.M., Wang,Y.W., Cox,C.S. Jr. and Wade,C.E.
  TITLE     Influence of TRPM4 rs8104571 genotype on intracranial pressure and
            outcomes in African Americans with traumatic brain injury
  JOURNAL   Sci Rep 13 (1), 5815 (2023)
   PUBMED   37037835
  REMARK    GeneRIF: Influence of TRPM4 rs8104571 genotype on intracranial
            pressure and outcomes in African Americans with traumatic brain
            injury.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4058)
  AUTHORS   Launay,P., Cheng,H., Srivatsan,S., Penner,R., Fleig,A. and
            Kinet,J.P.
  TITLE     TRPM4 regulates calcium oscillations after T cell activation
  JOURNAL   Science 306 (5700), 1374-1377 (2004)
   PUBMED   15550671
  REMARK    GeneRIF: TRPM4-mediated depolarization modulates Ca2+ oscillations,
            with downstream effects on cytokine production in T lymphocytes
REFERENCE   6  (bases 1 to 4058)
  AUTHORS   Nilius,B., Prenen,J., Droogmans,G., Voets,T., Vennekens,R.,
            Freichel,M., Wissenbach,U. and Flockerzi,V.
  TITLE     Voltage dependence of the Ca2+-activated cation channel TRPM4
  JOURNAL   J Biol Chem 278 (33), 30813-30820 (2003)
   PUBMED   12799367
  REMARK    GeneRIF: Voltage dependence is not due to block by divalent cations
            or to voltage-dependent binding of intracellular Ca2+ to an
            activator site, indicating that TRPM4 is a transient receptor
            potential channel with an intrinsic voltage-sensing mechanism.
REFERENCE   7  (bases 1 to 4058)
  AUTHORS   Hofmann,T., Chubanov,V., Gudermann,T. and Montell,C.
  TITLE     TRPM5 is a voltage-modulated and Ca(2+)-activated monovalent
            selective cation channel
  JOURNAL   Curr Biol 13 (13), 1153-1158 (2003)
   PUBMED   12842017
REFERENCE   8  (bases 1 to 4058)
  AUTHORS   Launay,P., Fleig,A., Perraud,A.L., Scharenberg,A.M., Penner,R. and
            Kinet,J.P.
  TITLE     TRPM4 is a Ca2+-activated nonselective cation channel mediating
            cell membrane depolarization
  JOURNAL   Cell 109 (3), 397-407 (2002)
   PUBMED   12015988
REFERENCE   9  (bases 1 to 4058)
  AUTHORS   Xu,X.Z., Moebius,F., Gill,D.L. and Montell,C.
  TITLE     Regulation of melastatin, a TRP-related protein, through
            interaction with a cytoplasmic isoform
  JOURNAL   Proc Natl Acad Sci U S A 98 (19), 10692-10697 (2001)
   PUBMED   11535825
REFERENCE   10 (bases 1 to 4058)
  AUTHORS   Brugada,R., Campuzano,O., Sarquella-Brugada,G., Brugada,P.,
            Brugada,J. and Hong,K.
  TITLE     Brugada Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301690
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA218254.1 and AF497623.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_017636.3.
            
            Summary: The protein encoded by this gene is a calcium-activated
            nonselective ion channel that mediates transport of monovalent
            cations across membranes, thereby depolarizing the membrane. The
            activity of the encoded protein increases with increasing
            intracellular calcium concentration, but this channel does not
            transport calcium. [provided by RefSeq, Mar 2016].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF497623.1, AK292862.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000252826.10/ ENSP00000252826.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 DA218254.1         34-36
            4-4058              AF497623.1         1-4055
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.33"
     gene            1..4058
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /note="transient receptor potential cation channel
                     subfamily M member 4"
                     /db_xref="GeneID:54795"
                     /db_xref="HGNC:HGNC:17993"
                     /db_xref="MIM:606936"
     exon            1..99
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     CDS             76..3720
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /note="isoform 1 is encoded by transcript variant 1;
                     melastatin-4; long transient receptor potential channel 4;
                     calcium-activated non-selective cation channel 1"
                     /codon_start=1
                     /product="transient receptor potential cation channel
                     subfamily M member 4 isoform 1"
                     /protein_id="NP_060106.2"
                     /db_xref="CCDS:CCDS33073.1"
                     /db_xref="GeneID:54795"
                     /db_xref="HGNC:HGNC:17993"
                     /db_xref="MIM:606936"
                     /translation="MVVPEKEQSWIPKIFKKKTCTTFIVDSTDPGGTLCQCGRPRTAH
                     PAVAMEDAFGAAVVTVWDSDAHTTEKPTDAYGELDFTGAGRKHSNFLRLSDRTDPAAV
                     YSLVTRTWGFRAPNLVVSVLGGSGGPVLQTWLQDLLRRGLVRAAQSTGAWIVTGGLHT
                     GIGRHVGVAVRDHQMASTGGTKVVAMGVAPWGVVRNRDTLINPKGSFPARYRWRGDPE
                     DGVQFPLDYNYSAFFLVDDGTHGCLGGENRFRLRLESYISQQKTGVGGTGIDIPVLLL
                     LIDGDEKMLTRIENATQAQLPCLLVAGSGGAADCLAETLEDTLAPGSGGARQGEARDR
                     IRRFFPKGDLEVLQAQVERIMTRKELLTVYSSEDGSEEFETIVLKALVKACGSSEASA
                     YLDELRLAVAWNRVDIAQSELFRGDIQWRSFHLEASLMDALLNDRPEFVRLLISHGLS
                     LGHFLTPMRLAQLYSAAPSNSLIRNLLDQASHSAGTKAPALKGGAAELRPPDVGHVLR
                     MLLGKMCAPRYPSGGAWDPHPGQGFGESMYLLSDKATSPLSLDAGLGQAPWSDLLLWA
                     LLLNRAQMAMYFWEMGSNAVSSALGACLLLRVMARLEPDAEEAARRKDLAFKFEGMGV
                     DLFGECYRSSEVRAARLLLRRCPLWGDATCLQLAMQADARAFFAQDGVQSLLTQKWWG
                     DMASTTPIWALVLAFFCPPLIYTRLITFRKSEEEPTREELEFDMDSVINGEGPVGTAD
                     PAEKTPLGVPRQSGRPGCCGGRCGGRRCLRRWFHFWGAPVTIFMGNVVSYLLFLLLFS
                     RVLLVDFQPAPPGSLELLLYFWAFTLLCEELRQGLSGGGGSLASGGPGPGHASLSQRL
                     RLYLADSWNQCDLVALTCFLLGVGCRLTPGLYHLGRTVLCIDFMVFTVRLLHIFTVNK
                     QLGPKIVIVSKMMKDVFFFLFFLGVWLVAYGVATEGLLRPRDSDFPSILRRVFYRPYL
                     QIFGQIPQEDMDVALMEHSNCSSEPGFWAHPPGAQAGTCVSQYANWLVVLLLVIFLLV
                     ANILLVNLLIAMFSYTFGKVQGNSDLYWKAQRYRLIREFHSRPALAPPFIVISHLRLL
                     LRQLCRRPRSPQPSSPALEHFRVYLSKEAERKLLTWESVHKENFLLARARDKRESDSE
                     RLKRTSQKVDLALKQLGHIREYEQRLKVLEREVQQCSRVLGWVAEALSRSALLPPGGP
                     PPPDLPGSKD"
     exon            100..167
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            168..342
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            343..523
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            524..687
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            688..871
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            872..933
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            934..1125
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1225
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1338
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            1339..1683
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            1684..1818
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            1819..1948
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            1949..2094
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            2095..2207
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            2208..2285
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            2286..2720
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            2721..2853
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            2854..3028
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            3029..3206
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            3207..3403
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            3404..3536
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            3537..3609
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            3610..3715
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     exon            3716..4058
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4038..4043
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /note="hexamer: AATAAA"
     polyA_site      4058
                     /gene="TRPM4"
                     /gene_synonym="EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B"
                     /note="major polyA site"
ORIGIN      
        1 gcgggtctgg aagcagagcc ggcggaggga gcgccggggc cctgggctgc aggaggttgc
       61 ggcggccgcg gcagcatggt ggtgccggag aaggagcaga gctggatccc caagatcttc
      121 aagaagaaga cctgcacgac gttcatagtt gactccacag atccgggagg gaccttgtgc
      181 cagtgtgggc gcccccggac cgcccacccc gcagtggcca tggaggatgc cttcggggca
      241 gccgtggtga ccgtgtggga cagcgatgca cacaccacgg agaagcccac cgatgcctac
      301 ggagagctgg acttcacggg ggccggccgc aagcacagca atttcctccg gctctctgac
      361 cgaacggatc cagctgcagt ttatagtctg gtcacacgca catggggctt ccgtgccccg
      421 aacctggtgg tgtcagtgct ggggggatcg gggggccccg tcctccagac ctggctgcag
      481 gacctgctgc gtcgtgggct ggtgcgggct gcccagagca caggagcctg gattgtcact
      541 gggggtctgc acacgggcat cggccggcat gttggtgtgg ctgtacggga ccatcagatg
      601 gccagcactg ggggcaccaa ggtggtggcc atgggtgtgg ccccctgggg tgtggtccgg
      661 aatagagaca ccctcatcaa ccccaagggc tcgttccctg cgaggtaccg gtggcgcggt
      721 gacccggagg acggggtcca gtttcccctg gactacaact actcggcctt cttcctggtg
      781 gacgacggca cacacggctg cctggggggc gagaaccgct tccgcttgcg cctggagtcc
      841 tacatctcac agcagaagac gggcgtggga gggactggaa ttgacatccc tgtcctgctc
      901 ctcctgattg atggtgatga gaagatgttg acgcgaatag agaacgccac ccaggctcag
      961 ctcccatgtc tcctcgtggc tggctcaggg ggagctgcgg actgcctggc ggagaccctg
     1021 gaagacactc tggccccagg gagtggggga gccaggcaag gcgaagcccg agatcgaatc
     1081 aggcgtttct ttcccaaagg ggaccttgag gtcctgcagg cccaggtgga gaggattatg
     1141 acccggaagg agctcctgac agtctattct tctgaggatg ggtctgagga attcgagacc
     1201 atagttttga aggcccttgt gaaggcctgt gggagctcgg aggcctcagc ctacctggat
     1261 gagctgcgtt tggctgtggc ttggaaccgc gtggacattg cccagagtga actctttcgg
     1321 ggggacatcc aatggcggtc cttccatctc gaagcttccc tcatggacgc cctgctgaat
     1381 gaccggcctg agttcgtgcg cttgctcatt tcccacggcc tcagcctggg ccacttcctg
     1441 accccgatgc gcctggccca actctacagc gcggcgccct ccaactcgct catccgcaac
     1501 cttttggacc aggcgtccca cagcgcaggc accaaagccc cagccctaaa agggggagct
     1561 gcggagctcc ggccccctga cgtggggcat gtgctgagga tgctgctggg gaagatgtgc
     1621 gcgccgaggt acccctccgg gggcgcctgg gaccctcacc caggccaggg cttcggggag
     1681 agcatgtatc tgctctcgga caaggccacc tcgccgctct cgctggatgc tggcctcggg
     1741 caggccccct ggagcgacct gcttctttgg gcactgttgc tgaacagggc acagatggcc
     1801 atgtacttct gggagatggg ttccaatgca gtttcctcag ctcttggggc ctgtttgctg
     1861 ctccgggtga tggcacgcct ggagcctgac gctgaggagg cagcacggag gaaagacctg
     1921 gcgttcaagt ttgaggggat gggcgttgac ctctttggcg agtgctatcg cagcagtgag
     1981 gtgagggctg cccgcctcct cctccgtcgc tgcccgctct ggggggatgc cacttgcctc
     2041 cagctggcca tgcaagctga cgcccgtgcc ttctttgccc aggatggggt acagtctctg
     2101 ctgacacaga agtggtgggg agatatggcc agcactacac ccatctgggc cctggttctc
     2161 gccttctttt gccctccact catctacacc cgcctcatca ccttcaggaa atcagaagag
     2221 gagcccacac gggaggagct agagtttgac atggatagtg tcattaatgg ggaagggcct
     2281 gtcgggacgg cggacccagc cgagaagacg ccgctggggg tcccgcgcca gtcgggccgt
     2341 ccgggttgct gcgggggccg ctgcgggggg cgccggtgcc tacgccgctg gttccacttc
     2401 tggggcgcgc cggtgaccat cttcatgggc aacgtggtca gctacctgct gttcctgctg
     2461 cttttctcgc gggtgctgct cgtggatttc cagccggcgc cgcccggctc cctggagctg
     2521 ctgctctatt tctgggcttt cacgctgctg tgcgaggaac tgcgccaggg cctgagcgga
     2581 ggcgggggca gcctcgccag cgggggcccc gggcctggcc atgcctcact gagccagcgc
     2641 ctgcgcctct acctcgccga cagctggaac cagtgcgacc tagtggctct cacctgcttc
     2701 ctcctgggcg tgggctgccg gctgaccccg ggtttgtacc acctgggccg cactgtcctc
     2761 tgcatcgact tcatggtttt cacggtgcgg ctgcttcaca tcttcacggt caacaaacag
     2821 ctggggccca agatcgtcat cgtgagcaag atgatgaagg acgtgttctt cttcctcttc
     2881 ttcctcggcg tgtggctggt agcctatggc gtggccacgg aggggctcct gaggccacgg
     2941 gacagtgact tcccaagtat cctgcgccgc gtcttctacc gtccctacct gcagatcttc
     3001 gggcagattc cccaggagga catggacgtg gccctcatgg agcacagcaa ctgctcgtcg
     3061 gagcccggct tctgggcaca ccctcctggg gcccaggcgg gcacctgcgt ctcccagtat
     3121 gccaactggc tggtggtgct gctcctcgtc atcttcctgc tcgtggccaa catcctgctg
     3181 gtcaacttgc tcattgccat gttcagttac acattcggca aagtacaggg caacagcgat
     3241 ctctactgga aggcgcagcg ttaccgcctc atccgggaat tccactctcg gcccgcgctg
     3301 gccccgccct ttatcgtcat ctcccacttg cgcctcctgc tcaggcaatt gtgcaggcga
     3361 ccccggagcc cccagccgtc ctccccggcc ctcgagcatt tccgggttta cctttctaag
     3421 gaagccgagc ggaagctgct aacgtgggaa tcggtgcata aggagaactt tctgctggca
     3481 cgcgctaggg acaagcggga gagcgactcc gagcgtctga agcgcacgtc ccagaaggtg
     3541 gacttggcac tgaaacagct gggacacatc cgcgagtacg aacagcgcct gaaagtgctg
     3601 gagcgggagg tccagcagtg tagccgcgtc ctggggtggg tggccgaggc cctgagccgc
     3661 tctgccttgc tgcccccagg tgggccgcca ccccctgacc tgcctgggtc caaagactga
     3721 gccctgctgg cggacttcaa ggagaagccc ccacagggga ttttgctcct agagtaaggc
     3781 tcatctgggc ctcggccccc gcacctggtg gccttgtcct tgaggtgagc cccatgtcca
     3841 tctgggccac tgtcaggacc acctttggga gtgtcatcct tacaaaccac agcatgcccg
     3901 gctcctccca gaaccagtcc cagcctggga ggatcaaggc ctggatcccg ggccgttatc
     3961 catctggagg ctgcagggtc cttggggtaa cagggaccac agacccctca ccactcacag
     4021 attcctcaca ctggggaaat aaagccattt cagaggaa
//
