LOCUS       NM_001366145           12320 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens transient receptor potential cation channel subfamily
            M member 3 (TRPM3), transcript variant 14, mRNA.
ACCESSION   NM_001366145 XM_011519041
VERSION     NM_001366145.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12320)
  AUTHORS   Behrendt,M.
  TITLE     Implications of TRPM3 and TRPM8 for sensory neuron sensitisation
  JOURNAL   Biol Chem 405 (9-10), 583-599 (2024)
   PUBMED   39417661
  REMARK    GeneRIF: Implications of TRPM3 and TRPM8 for sensory neuron
            sensitisation.
            Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12320)
  AUTHORS   Marshall-Gradisnik,S., Martini Sasso,E., Eaton-Fitch,N., Smith,P.,
            Baraniuk,J.N. and Muraki,K.
  TITLE     Novel characterization of endogenous transient receptor potential
            melastatin 3 ion channels from Gulf War Illness participants
  JOURNAL   PLoS One 19 (6), e0305704 (2024)
   PUBMED   38917121
  REMARK    GeneRIF: Novel characterization of endogenous transient receptor
            potential melastatin 3 ion channels from Gulf War Illness
            participants.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 12320)
  AUTHORS   Zhou,Y., Bennett,T.M., Ruzycki,P.A., Guo,Z., Cao,Y.Q.,
            Shahidullah,M., Delamere,N.A. and Shiels,A.
  TITLE     A Cataract-Causing Mutation in the TRPM3 Cation Channel Disrupts
            Calcium Dynamics in the Lens
  JOURNAL   Cells 13 (3), 257 (2024)
   PUBMED   38334649
  REMARK    GeneRIF: A Cataract-Causing Mutation in the TRPM3 Cation Channel
            Disrupts Calcium Dynamics in the Lens.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 12320)
  AUTHORS   Kashio,M. and Tominaga,M.
  TITLE     Role of novel de novo gain-of-function TRPM3 mutations in a
            spectrum of neurodevelopmental disorders
  JOURNAL   Cell Calcium 110, 102704 (2023)
   PUBMED   36804759
  REMARK    GeneRIF: Role of novel de novo gain-of-function TRPM3 mutations in
            a spectrum of neurodevelopmental disorders.
REFERENCE   5  (bases 1 to 12320)
  AUTHORS   Burglen,L., Van Hoeymissen,E., Qebibo,L., Barth,M., Belnap,N.,
            Boschann,F., Depienne,C., De Clercq,K., Douglas,A.G.L.,
            Fitzgerald,M.P., Foulds,N., Garel,C., Helbig,I., Held,K., Horn,D.,
            Janssen,A., Kaindl,A.M., Narayanan,V., Prager,C., Rupin-Mas,M.,
            Afenjar,A., Zhao,S., Ramaekers,V.T., Ruggiero,S.M., Thomas,S.,
            Valence,S., Van Maldergem,L., Rohacs,T., Rodriguez,D., Dyment,D.,
            Voets,T. and Vriens,J.
  TITLE     Gain-of-function variants in the ion channel gene TRPM3 underlie a
            spectrum of neurodevelopmental disorders
  JOURNAL   Elife 12, e81032 (2023)
   PUBMED   36648066
  REMARK    GeneRIF: Gain-of-function variants in the ion channel gene TRPM3
            underlie a spectrum of neurodevelopmental disorders.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 12320)
  AUTHORS   Oberwinkler,J., Lis,A., Giehl,K.M., Flockerzi,V. and Philipp,S.E.
  TITLE     Alternative splicing switches the divalent cation selectivity of
            TRPM3 channels
  JOURNAL   J Biol Chem 280 (23), 22540-22548 (2005)
   PUBMED   15824111
  REMARK    GeneRIF: the divalent cation selectivity of TRPM3 channels is
            regulated by altenrative splicing
REFERENCE   7  (bases 1 to 12320)
  AUTHORS   Grimm,C., Kraft,R., Schultz,G. and Harteneck,C.
  TITLE     Activation of the melastatin-related cation channel TRPM3 by
            D-erythro-sphingosine [corrected]
  JOURNAL   Mol Pharmacol 67 (3), 798-805 (2005)
   PUBMED   15550678
  REMARK    GeneRIF: TRPM3 is the first ion channel activated by sphingolipids.
            Erratum:[Mol Pharmacol. 2005 Apr;67(4):1382]
REFERENCE   8  (bases 1 to 12320)
  AUTHORS   Grimm,C., Kraft,R., Sauerbruch,S., Schultz,G. and Harteneck,C.
  TITLE     Molecular and functional characterization of the melastatin-related
            cation channel TRPM3
  JOURNAL   J Biol Chem 278 (24), 21493-21501 (2003)
   PUBMED   12672799
REFERENCE   9  (bases 1 to 12320)
  AUTHORS   Lee,N., Chen,J., Sun,L., Wu,S., Gray,K.R., Rich,A., Huang,M.,
            Lin,J.H., Feder,J.N., Janovitz,E.B., Levesque,P.C. and Blanar,M.A.
  TITLE     Expression and characterization of human transient receptor
            potential melastatin 3 (hTRPM3)
  JOURNAL   J Biol Chem 278 (23), 20890-20897 (2003)
   PUBMED   12672827
  REMARK    GeneRIF: The hTRPM3 gene is comprised of 24 exons and maps to
            chromosome 9q-21.12 and is composed of 1555 amino acids with the
            characteristic six-transmembrane domain of TRPs and is expressed in
            kidney and, at lesser levels, in brain, testis, and spinal cord
REFERENCE   10 (bases 1 to 12320)
  AUTHORS   Dyment,D., Lines,M. and Innes,A.M.
  TITLE     TRPM3-Related Neurodevelopmental Disorder
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   36821706
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL161913.11, AL356318.7,
            AL159990.12, AL358786.21 and AL442645.8.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001366145.1.
            
            Summary: The product of this gene belongs to the family of
            transient receptor potential (TRP) channels. TRP channels are
            cation-selective channels important for cellular calcium signaling
            and homeostasis. The protein encoded by this gene mediates calcium
            entry, and this entry is potentiated by calcium store depletion.
            Alternatively spliced transcript variants encoding different
            isoforms have been identified. [provided by RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.3488.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1968189,
                                           SAMEA1968540 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000677713.2/ ENSP00000503830.2
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-444               AL161913.11        108546-108989       c
            445-524             AL356318.7         31652-31731         c
            525-729             AL356318.7         30128-30332         c
            730-943             AL356318.7         13598-13811         c
            944-1068            AL356318.7         10223-10347         c
            1069-1240           AL159990.12        143800-143971       c
            1241-1415           AL159990.12        100058-100232       c
            1416-1539           AL159990.12        77554-77677         c
            1540-1612           AL358786.21        118166-118238       c
            1613-1713           AL358786.21        77220-77320         c
            1714-1848           AL358786.21        75720-75854         c
            1849-1899           AL358786.21        71871-71921         c
            1900-1935           AL358786.21        62142-62177         c
            1936-2076           AL358786.21        61851-61991         c
            2077-2106           AL358786.21        57904-57933         c
            2107-2396           AL358786.21        56736-57025         c
            2397-2625           AL358786.21        55527-55755         c
            2626-2793           AL358786.21        52568-52735         c
            2794-2934           AL358786.21        47269-47409         c
            2935-3063           AL358786.21        40002-40130         c
            3064-3315           AL358786.21        35079-35330         c
            3316-3490           AL358786.21        27691-27865         c
            3491-3641           AL442645.8         138504-138654       c
            3642-3841           AL442645.8         138188-138387       c
            3842-3974           AL442645.8         134886-135018       c
            3975-12320          AL442645.8         114404-122749       c
FEATURES             Location/Qualifiers
     source          1..12320
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q21.12-q21.13"
     gene            1..12320
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /note="transient receptor potential cation channel
                     subfamily M member 3"
                     /db_xref="GeneID:80036"
                     /db_xref="HGNC:HGNC:17992"
                     /db_xref="MIM:608961"
     exon            1..444
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     CDS             268..5427
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /note="isoform m is encoded by transcript variant 14; long
                     transient receptor potential channel 3; melastatin-2"
                     /codon_start=1
                     /product="transient receptor potential cation channel
                     subfamily M member 3 isoform m"
                     /protein_id="NP_001353074.1"
                     /db_xref="CCDS:CCDS94420.1"
                     /db_xref="GeneID:80036"
                     /db_xref="HGNC:HGNC:17992"
                     /db_xref="MIM:608961"
                     /translation="MPEPWGTVYFLGIAQVFSFLFSWWNLEGVMNQADAPRPLNWTIR
                     KLCHAAFLPSVRLLKAQKSWIERAFYKRECVHIIPSTKDPHRCCCGRLIGQHVGLTPS
                     ISVLQNEKNESRLSRNDIQSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSF
                     DTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWI
                     FTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLIGRDVVRPYQTMS
                     NPMSKLTVLNSMHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVA
                     LIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGLINESLRDQ
                     LLVTIQKTFTYTRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGA
                     NASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQAMLDALVLDRVDFVKLLIEN
                     GVSMHRFLTISRLEELYNTRHGPSNTLYHLVRDVKKREYPGFGWIYFKGNLPPDYRIS
                     LIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDIPLRRGRKT
                     TKKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVAC
                     KLCKAMAHEASENDMVDDISQELNHNSRDFGQLAVELLDQSYKQDEQLAMKLLTYELK
                     NWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGILLPPSIL
                     SLEFKNKDDMPYMSQAQEIHLQEKEAEEPEKPTKEKEEEDMELTAMLGRNNGESSRKK
                     DEEEVQSKHRLIPLGRKIYEFYNAPIVKFWFYTLAYIGYLMLFNYIVLVKMERWPSTQ
                     EWIVISYIFTLGIEKMREILMSEPGKLLQKVKVWLQEYWNVTDLIAILLFSVGMILRL
                     QDQPFRSDGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVV
                     LMSFGVARQAILFPNEEPSWKLAKNIFYMPYWMIYGEVFADQIDPPCGQNETREDGKI
                     IQLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQL
                     IMTFHERPVLPPPLIIFSHMTMIFQHLCCRWRKHESDPDERDYGLKLFITDDELKKVH
                     DFEEQCIEEYFREKDDRFNSSNDERIRVTSERVENMSMRLEEVNEREHSMKASLQTVD
                     IRLAQLEDLIGRMATALERLTGLERAESNKIRSRTSSDCTDAAYIVRQSSFNSQEGNT
                     FKLQESIDPAGEETMSPTSPTLMPRMRSHSFYSVNMKDKGGIEKLESIFKERSLSLHR
                     ATSSHSVAKEPKAPAAPANTLAIVPDSRRPSSCIDIYVSAMDELHCDIDPLDNSVNIL
                     GLGEPSFSTPVPSTAPSSSAYATLAPTDRPPSRSIDFEDITSMDTRSFSSDYTHLPEC
                     QNPWDSEPPMYHTIERSKSSRYLATTPFLLEEAPIVKSHSFMFSPSRSYYANFGVPVK
                     TAEYTSITDCIDTRCVNAPQAIADRAAFPGGLGDKVEDLTCCHPEREAELSHPSSDSE
                     ENEAKGRRATIAISSQEGDNSERTLSNNITVPKIERANSYSAEEPSAPYAHTRKSFSI
                     SDKLDRQRNTASLRNPFQRSKSSKPEGRGDSLSMRRLSRTSAFQSFESKHN"
     exon            445..524
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            525..729
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            730..943
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1068
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1240
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1415
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            1416..1539
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1612
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            1613..1713
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            1714..1848
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            1849..1899
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            1900..1935
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            1936..2076
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            2077..2106
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            2107..2396
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            2397..2625
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            2626..2793
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            2794..2934
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            2935..3063
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            3064..3315
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            3316..3490
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            3491..3641
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            3642..3841
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            3842..3974
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     exon            3975..12320
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      12287..12292
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /note="hexamer: AATAAA"
     polyA_site      12320
                     /gene="TRPM3"
                     /gene_synonym="CTRCT50; GON-2; LTRPC3; MLSN2; NEDFSS"
                     /note="major polyA site"
ORIGIN      
        1 attctggagc aggctgcttt gactccgacc acaggctgtt ttgtgcaggc tgtccctctt
       61 cttcaaaatc gtgcatcccc tccccgaagc agcaggcagt gtgcctccat tcagccacat
      121 ttggtatgca tgagcacggc tgcagagaga ggggaggtgg ctgttttaag aaggttcagg
      181 ggctcaggca aggctacttg actagtcttc caagttccag gaagcctctg ccctaatgga
      241 atttgcaggt gtggagatga ccatgggatg ccagagccgt gggggaccgt ttattttcta
      301 ggcattgctc aggttttcag tttcttgttt tcctggtgga atttggaagg ggtcatgaat
      361 caggctgatg ctcctcgacc cctaaactgg accatccgga agctgtgcca cgcagccttt
      421 cttccatctg tcagacttct gaaggctcag aaatcctgga tagaaagagc attttataaa
      481 agagaatgtg tccacatcat acccagcacc aaagaccccc ataggtgttg ctgtgggcgt
      541 ctgataggcc agcatgttgg cctcaccccc agtatctccg tgcttcagaa tgagaaaaat
      601 gaaagtcgcc tctcccgaaa tgacatccag tctgaaaagt ggtccatcag caaacacact
      661 caactcagcc ctacggatgc ttttgggacc attgagttcc aaggaggtgg ccattccaac
      721 aaagccatgt atgtgcgagt atcttttgat acaaaacctg atctcctctt acacctgatg
      781 accaaggaat ggcagttgga gcttcccaag cttctcatct ctgtccatgg gggcctgcag
      841 aactttgaac tccagccaaa actcaagcaa gtctttggga aagggctcat caaagcagca
      901 atgacaactg gagcgtggat attcactgga ggggttaaca caggtgttat tcgtcatgtt
      961 ggcgatgcct tgaaggatca tgcctctaag tctcgaggaa agatatgcac cataggtatt
     1021 gccccctggg gaattgtgga aaaccaggag gacctcattg gaagagatgt tgtccggcca
     1081 taccagacca tgtccaatcc catgagcaag ctcactgttc tcaacagcat gcattcccac
     1141 ttcattctgg ctgacaacgg gaccactgga aaatatggag cagaggtgaa acttcgaaga
     1201 caactggaaa agcatatttc actccagaag ataaacacaa gaatcggtca aggtgttcct
     1261 gtggtggcac tcatagtgga aggaggaccc aatgtgatct cgattgtttt ggagtacctt
     1321 cgagacaccc ctcccgtgcc agtggttgtc tgtgatggga gtggacgggc atcggacatc
     1381 ctggcctttg ggcataaata ctcagaagaa ggcggactga taaatgaatc tttgagggac
     1441 cagctgttgg tgactataca gaagactttc acatacactc gaacccaagc tcagcatctg
     1501 ttcatcatcc tcatggagtg catgaagaag aaggaattga ttacggtatt tcggatggga
     1561 tcagaaggac accaggacat tgatttggct atcctgacag ctttactcaa aggagccaat
     1621 gcctcggccc cagaccaact gagcttagct ttagcctgga acagagtcga catcgctcgc
     1681 agccagatct ttatttacgg gcaacagtgg ccggtgggat ctctggagca agccatgttg
     1741 gatgccttag ttctggacag agtggatttt gtgaaattac tcatagagaa tggagtaagc
     1801 atgcaccgtt ttctcaccat ctccagacta gaggaattgt acaatacgag acatgggccc
     1861 tcaaatacat tgtaccactt ggtcagggat gtcaaaaagc gagagtatcc aggtttcggt
     1921 tggatctatt ttaaggggaa cctgccccca gactacagaa tcagcctgat tgacatcggc
     1981 ctggtgatcg agtacctgat gggcggggct tatcgctgca actacacgcg caagcgcttc
     2041 cggaccctct accacaacct cttcggcccc aagaggccca aagccttgaa actgctggga
     2101 atggaggatg atattccctt gaggcgagga agaaagacaa ccaagaaacg tgaagaagag
     2161 gtggacattg acttggatga tcctgagatc aaccacttcc ccttcccttt ccatgagctc
     2221 atggtgtggg ctgttctcat gaagcggcag aagatggccc tgttcttctg gcagcacggt
     2281 gaggaggcca tggccaaggc cctggtggcc tgcaagctct gcaaagccat ggctcatgag
     2341 gcctctgaga acgacatggt tgacgacatt tcccaggagc tgaatcacaa ttccagagac
     2401 tttggccagc tggctgtgga gctcctggac cagtcctaca agcaggacga acagctggcc
     2461 atgaaactgc tgacgtatga gctgaagaac tggagcaacg ccacgtgcct gcagcttgcc
     2521 gtggctgcca aacaccgcga cttcatcgcg cacacgtgca gccagatgct gctcaccgac
     2581 atgtggatgg gccggctccg catgcgcaag aactcaggcc tcaaggtaat tctgggaatt
     2641 ctacttcctc cttcaattct cagcttggag ttcaagaaca aagacgacat gccctatatg
     2701 tctcaggccc aggaaatcca cctccaagag aaggaggcag aagaaccaga gaagcccaca
     2761 aaggaaaaag aggaagagga catggagctc acagcaatgt tgggacgaaa caacggggag
     2821 tcctccagga agaaggatga agaggaagtt cagagcaagc accggttaat ccccctcggc
     2881 agaaaaatct atgaattcta caatgcaccc atcgtgaagt tctggttcta cacactggcg
     2941 tatatcggat acctgatgct cttcaactat atcgtgttag tgaagatgga acgctggccg
     3001 tccacccagg aatggatcgt aatctcctat attttcaccc tgggaataga aaagatgaga
     3061 gagattctga tgtcagagcc agggaagttg ctacagaaag tgaaggtatg gctgcaggag
     3121 tactggaatg tcacggacct catcgccatc cttctgtttt ctgtcggaat gatccttcgt
     3181 ctccaagacc agcccttcag gagtgacggg agggtcatct actgcgtgaa catcatttac
     3241 tggtatatcc gtctcctaga catcttcggc gtgaacaagt atttgggccc gtatgtaatg
     3301 atgattggaa aaatgatgat agacatgatg tactttgtca tcattatgct ggtggttctg
     3361 atgagctttg gggtcgccag gcaagccatc ctttttccca atgaggagcc atcatggaaa
     3421 ctggccaaga acatcttcta catgccctat tggatgattt atggggaagt gtttgcggac
     3481 cagatagacc ctccctgtgg acagaatgag acccgagagg atggtaaaat aatccagctg
     3541 cctccctgca agacaggagc ttggatcgtg ccggccatca tggcctgcta cctcttagtg
     3601 gcaaacatct tgctggtcaa cctcctcatt gctgtcttta acaatacatt ttttgaagta
     3661 aaatcgatat ccaaccaagt ctggaagttt cagaggtatc agctcatcat gactttccat
     3721 gaaaggccag ttctgccccc accactgatc atcttcagcc acatgaccat gatattccag
     3781 cacctgtgct gccgatggag gaaacacgag agcgacccgg atgaaaggga ctacggcctg
     3841 aaactcttca taaccgatga tgagctcaag aaagtacatg actttgaaga gcaatgcata
     3901 gaagaatact tcagagaaaa ggatgatcgg ttcaactcat ctaatgatga gaggatacgg
     3961 gtgacttcag aaagggtgga gaacatgtct atgcggctgg aggaagtcaa cgagagagag
     4021 cactccatga aggcttcact ccagaccgtg gacatccggc tggcgcagct ggaagacctt
     4081 atcgggcgca tggccacggc cctggagcgc ctgacaggtc tggagcgggc cgagtccaac
     4141 aaaatccgct cgaggacctc gtcagactgc acggacgccg cctacattgt ccgtcagagc
     4201 agcttcaaca gccaggaagg gaacaccttc aagctccaag agagtataga ccctgcaggt
     4261 gaggagacca tgtccccaac ttctccaacc ttaatgcccc gtatgcgaag ccattctttc
     4321 tattcggtca atatgaaaga caaaggtggt atagaaaagt tggaaagtat ttttaaagaa
     4381 aggtccctga gcctacaccg ggctactagt tcccactctg tagcaaaaga acccaaagct
     4441 cctgcagccc ctgccaacac cttggccatt gttcctgatt ccagaagacc atcatcgtgt
     4501 atagacatct atgtctctgc tatggatgag ctccactgtg atatagaccc tctggacaat
     4561 tccgtgaaca tccttgggct gggcgagcca agcttttcaa ctccagtacc ttccacagcc
     4621 ccttcaagta gtgcctatgc aacacttgca cccacagaca gacctccaag ccggagcatt
     4681 gattttgagg acatcacctc catggacact agatcttttt cttcagacta cacccacctc
     4741 ccagaatgcc aaaacccctg ggactcagag cctccgatgt accacaccat tgagcgttcc
     4801 aaaagtagcc gctacctagc caccacaccc tttcttctag aagaggctcc cattgtgaaa
     4861 tctcatagct ttatgttttc cccctcaagg agctattatg ccaactttgg ggtgcctgta
     4921 aaaacagcag aatacacaag tattacagac tgtattgaca caaggtgtgt caatgcccct
     4981 caagcaattg cggacagagc tgccttccct ggaggtcttg gagacaaagt ggaggactta
     5041 acttgctgcc atccagagcg agaagcagaa ctgagtcacc ccagctctga cagtgaggag
     5101 aatgaggcca aaggccgcag agccaccatt gcaatatcct cccaggaggg tgataactca
     5161 gagagaaccc tgtccaacaa catcactgtt cccaagatag agcgcgccaa cagctactcg
     5221 gcagaggagc caagtgcgcc atatgcacac accaggaaga gcttctccat cagtgacaaa
     5281 ctcgacaggc agcggaacac agcaagcctg cgaaatccct tccagagaag caagtcctcc
     5341 aagccggagg gccgagggga cagcctgtcc atgaggagac tgtccagaac atcggctttc
     5401 caaagctttg aaagcaagca caactaaacc ttcttaatat ccgccacaga aggctcaaga
     5461 atccagccct aaaattctct ccaactccag tttttcccct ttccttgaat catacctgct
     5521 ttattcttag ctgagcaaaa caagcaatgc tttgggaggt gttaactcaa aggtgacttc
     5581 tgggccacag atcaagaaag catttgatct gacccagtgc cagacacagg ggatttaagg
     5641 catgttcaca cttgctgggc agggaggggg aagagaggga gaaggaaggg ttagagatga
     5701 atgtgtatcc gcagtcacag cagaaagcca tgagagcagg ggaaacaagg ggcttcgagc
     5761 acgctccatg ccaggaggca tctgttgatt tctgaccatt atcaagagtt gtaggatgca
     5821 gggctaaatt gcaaaataaa ataaaatagc cagcgtacac aatgagatat tctaaacttc
     5881 cattctgttt tcttttcaca ttggctccat cactggtgac tgatgaagag catcctcttt
     5941 attcagtata agccggcagc aagcagttct acctaacgtc ccacatcctt ctcatgccaa
     6001 cacttctgta attgatcatt ataaagaaaa aacaaggtaa cagtcatagt tcacctgtct
     6061 cttatctatt cacttctggt gccacaactg tttatccttt tttgaagaaa ataagggaac
     6121 agaaatgcct ttttgtattg caatcgaaat gaaagaagag ttgatgttaa aaaaacaaaa
     6181 gtcaagtgat ttattatata cagtgggcgt tcaagtctag tcgagcaagc tcaggagaat
     6241 gtaattaaat aattttatat tttttaattt attttgtatc tcacctgtca tggatgaatt
     6301 cattcactga atatgtaata ttgaacttaa aaaaaaaaaa aaaaaaacag tggacagaac
     6361 tagcctgaaa ttgccatgtg ggatgttctg ttctcaccgt tcatgtcgtg tgttgtgctg
     6421 ctgtgtgacc gtcatcattt gcatggctcc accacgtcct cctgaacacc tccaagacgt
     6481 aactgccaat ttttcacacc actcatcaca tgaccatttt gtatatgaat atatggttat
     6541 atgtggatat tttcatacct agagttttgc catctaatct gagctcagca taaatttaat
     6601 tggagttttt caagggctgg tatcttttct atggaaatgt tccaaagtac tttttaagga
     6661 agtttcaaaa ctataaataa ccttagattt gactgggagt ttggtatcaa cccaaagcca
     6721 tcagttgcaa gcataccatg aatatttttc ttatagacag agctataaaa aaagatacag
     6781 tagaccaaaa tagaaagcaa ataaaccata taaagagttc tatagtatat gccacttata
     6841 cacatgtata tgcatttatg cagagacaca tatttatata taaaataaat ggacataaag
     6901 agctttctta agaggcttgg acttttctat caccttgaag tataatcaag atttttttta
     6961 atatacaaga agttccattg aaagagttca ttatactatt agaccaaatg ttgaaattat
     7021 gaatttttta aattcctatg acttttcctc atatcaaact agagttgtca aaatgactca
     7081 ctgctcgcct tctgcccttt tttgtctgaa atcactcact atcaggacat ctcatttagt
     7141 ctcgcttttc ctaaacaaag tttttacacc acgtacaatc tgaatagaca cgcaacttta
     7201 aaccagctag tgttacagac tctaaagtag aggcataatc ttctagatca gaatctggat
     7261 tggaagtggg ataaagaaat cttgacccca aatctttgga ttcctatttg gctcattaac
     7321 gtttaaaaaa gaataaagcc tttaaggaaa acaatctatt gttcctggcc tgagatacta
     7381 cctggttctt ttgtttaatt ataattgaaa acaccaagtt ttactttatt ttgtgaaatc
     7441 aagtaaatac atggccttta aatataagat aaaaaacaaa ataaaaactc ttctgaataa
     7501 gcatttcagg tataaaaatt gtgatgaggt cacatatgta aagaattaaa caattccttt
     7561 ttcactcctt ggcatttagg tgaattgcta acaaacaccc agactccctt atttacagct
     7621 gttctagaaa cacttaacat tttattgggc cccacgctgg ctatttccct ctcagcaaac
     7681 atgcactggg tctgagaata aatcagtcgt tcttaaactt ccttgagtca tgaatcccta
     7741 cgaggctcta cgataactct aggctctctc tccagaaaga aaaaaaaaat gtacataatg
     7801 caaagttgca atataacttg aaagggttca tgagcccctg gttctatatt gattatgagg
     7861 gaaaggaacc atgtttgctc gtagttaatg cagttgtaat aggcacttta catttgagac
     7921 catctctctg tgattctcag gaaggccaaa tctcagcacc agagtttgaa caaagatttt
     7981 gttatttttg ttctttaaat tagatgttgc caatttaatg cacttacatc ctaacatgtt
     8041 gatgatttta agtatcaagc acaatcacaa tgctgaagtc tccctgaagg gtctatagag
     8101 gaatccaaga aggaggtgaa aacattattc attgacatca aacacctcca aggcctcatt
     8161 ctgggccaga ttggaagtga gggaaagaaa ttttcagctc aagtgtttcc attcatcgtg
     8221 atttcagaga gaaaaggata ggaactggga aacgaaatac tcaacagtcc tgatcttgca
     8281 attacctgat tcttttgttg tgcatgaaaa ttaagttcta acgtctgtga aacgtgtgaa
     8341 atatataagc caaacttaaa tgactgtaga cacaaactgt gtaacttcca tgagccattt
     8401 ccttcctgtg cacatgtatg tgtgttattt ttcagtccat ttccttccat ttctgggttc
     8461 tctgtgcagc aatgtggcat tgattctgtg gccttgtgca gtcagcttat gctcagccca
     8521 ttgattccac cacatccctg atcgagggga cgttaattac ctgtctttgt gaattaagca
     8581 gtttatgagc agcaagttac tgaggaccgt aaataaagtc atagagagct caaggaagta
     8641 atagcttcgg tttaatcatc tgcttcaaag gtgccaattt ttacatacta ctatatgtaa
     8701 tgatttgttc taaattgctt gttccttttg aaaaaaattt aaggatgttt tatgaaaatg
     8761 accatggagg tctcctgctt aaccaatata ctttatattt atagttagag ggcccctcct
     8821 tgatagattt gaatgaggag ctggaaagct tgggtacaat tttcatgact agtttgtatt
     8881 atctctgcaa gccaaaaggt gtattagcag tcagaataca gcactttaca gaactggatt
     8941 ttttaaatgt ataagtttcc aggtctagga tattatttag cactttgaaa gttccccttt
     9001 taaaatatat atataagaat aatcaaacga gaaaaatgta ctcataaaaa cacaaaatgt
     9061 gtttcttctg aatgtttcaa atgtattcta aaatatggtt tctatagata atattatggt
     9121 agataactgg atagtacttt tttactttct gagtctgtac agtctaatct tcctgaatgt
     9181 ttgggctgtt taatttcttt aaagatgata aatattaccc ggtcatccat catgctctat
     9241 ctataggcta taccattcat atgttgggca atgctgtatt ataaaacaca tgtaaacatg
     9301 tatgtaaccc atgtttaacc cagcgtagtt gtagcatgtg gttgtattaa tgaacgttac
     9361 aggagagctt tataatcatt tataaatctg taacattcaa taaagaaaca catgttgcaa
     9421 tgattaatta tgttcccaat tccatgttat ttagcatctt tgtgaaccgg gcatttttgt
     9481 tcctgatctg cttgatgctt ttcatgttcc tttggaccac tgtaaaatat gtgtgtaagc
     9541 tgatgtatat cctctaatga gtcctgcgag ctgtggtagg caagttgtga ttttgtgaaa
     9601 gatgaactgg catgttatgc aaaagactat atgacaaatg aacttaaaat gatattttaa
     9661 actccagttg aattagctgt ttacatctgc caaccccatg cagaagcagt gtacagtatt
     9721 ttataaatat gttcatttag taacactatg ttgtttctag ataaagtgat actagtgtgt
     9781 aggtggttat atatttggcc tagatattgc attataactc actatccttg aacaataaca
     9841 atgtgactga aatgaagtaa tctctctcca aacctggtct taattttgtg tcaacaaaat
     9901 gcatgcactg ctttatgcat ttcttttgac ttttctaagg cacttaacat tttactattg
     9961 cctgctgtga cgcttaagcc agttaagaat tcactcaaac agttgggctt cttactatgg
    10021 ggattatgtt cagttttgaa aaattaaaaa caaaaaaaaa gtaaccaagg gatttttaaa
    10081 tctttttttt aagcactgaa caaataatat gaaataaagt attaactgca ggttgtatag
    10141 ccatgggacc cagaggtggc aagccaacac ctcttgggtc tgccaaggtc taggtgttgt
    10201 gcatctgtga tatatgcaag acacatttct gattcttcag agctactggg tgcaaattgc
    10261 taagccggag atgagagcga aaagtccatt tgtgatagtg cagaagtgcg cagatcgctg
    10321 tgaatgtgtg atccaatgat atgacaagag acctccctcc ttgtcttgtg gcttcttcac
    10381 cttgggggtg ggaggcgtgt gtttgggtgt ctgatgaagc actgggtggt tctaaagagt
    10441 ttactctttt agaaaatcat cattactaga agccattcaa catctgttaa agagaggagc
    10501 caatttgcac agttttgaaa atgactgctc ctaaaatgag tagacaaaac tttcctctca
    10561 gcctgcattg acctaagctt tctcttcttt ttacaagttg gatattctgt ctctcagatt
    10621 ggagtcaacc aaaagaaagg ctggttccaa ttaaggaaaa gcaatttttt tcttttaatt
    10681 cctctcagaa ggtagttgtg ttaccttttc tctgatgatt tccttgcact catcactgct
    10741 aatgaaagca acgtgactgt ttctacaggt catctaagag aggattttgg tgaccaggta
    10801 atccagatgg caataagaat gaggtgaatt ttcccttcct taccttacct agtctaccag
    10861 cctccttaaa cctgcaggcc cttgtgcgtt tgcacaatgt ttgtgtgctg gaaactgaaa
    10921 agacatgtgt atggctagaa gtccataggc tttgtaaaga gtgtcagtgc caatcaggac
    10981 ccatgccttc ctctggagga gacaggtggc tgaggcctat ggggcaaagg gggaaattca
    11041 gccttgtgcc tggatgtgcg gctttctttc tcccctcttc ttgcttctct tctcttagtc
    11101 taagactagc tgctttttca ccagaccaac agacccttgc ccacattacc tttatacctt
    11161 gttgaccctc gcacagtaag ctgaaacagc ggccgcttgg aaaaaggcaa acatttagac
    11221 tttgtcccag gcctgatctt cccaccaact tagcctcctc ctgcctctac cccggctggt
    11281 gttcacattg acctcgtcca atttttgcct ctctaaatct caagagtcct acacatcttc
    11341 accgcagctt cccacacatc attaaaacca gccatctgca catagactga gaggctgcaa
    11401 attcaaacac atggaggggc cggatccaga aaacacaaat gtgtgaactg gactggtgaa
    11461 agtcaacaga agtagggttt gtaagtaagg catgacagaa tacacatccc accacaaaca
    11521 ccttcaaatt cagctttcca aacactgtcc tagccaaaca aacatgtctg ccagcagcat
    11581 tcagtcccaa aggcaatagt ttgggacctc tgatttagag ccaacaggat cagattcttg
    11641 atagtgactt ggtgagtgta cacacaaaat acactgagaa ccgagagggg ggaaaagaaa
    11701 ctcgccttat tcagagttac tcttgttctg aatgatatat gcatgtatat ttgttagcca
    11761 cataattcta aacttgaaca cagagtcttt gtgagagaga ttcagaagaa gagtaccata
    11821 gcatggtatg aagacttacc tgcttcctgt cactactgcg tctgacctga gtttatattg
    11881 ctgctaccgc tgtgtaaaaa cagtacgtgc agtatactgt actcatagcc atttcataat
    11941 ataggaccta gactctcatt tgtaaacgtt ctaaccctag tttataatgg gggaaattat
    12001 tcagatatca tttttaaatg aattcctaca aatacaatgg ttttaaaatt gggtttgctg
    12061 aatatatata catcttttcc ttctttgtgt atggatggac cacggtatgc atatctatcg
    12121 acactataaa tatatataaa tccttagaag aattgttctt ttttaaaggt ctattattgg
    12181 attcacaatg cactttgagt ttgattgttt agatatacaa ttcagagcag aagttggcag
    12241 ataccacggg aaaaactttc tgaaatttct gtaacaaatg ttttgcaata aaaaaaaaaa
    12301 ctcagtagtt taaaacatga
//
