LOCUS       NM_016179               7979 bp    mRNA    linear   PRI 12-DEC-2024
DEFINITION  Homo sapiens transient receptor potential cation channel subfamily
            C member 4 (TRPC4), transcript variant 1, mRNA.
ACCESSION   NM_016179
VERSION     NM_016179.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Chen,X., Yan,Y., Liu,Z., Yang,S., Li,W., Wang,Z., Wang,M., Guo,J.,
            Li,Z., Zhu,W., Yang,J., Yin,J., Dai,Q., Li,Y., Wang,C., Zhao,L.,
            Yang,X., Guo,X., Leng,L., Xu,J., Obukhov,A.G., Cao,R. and Zhong,W.
  TITLE     In vitro and in vivo inhibition of the host TRPC4 channel
            attenuates Zika virus infection
  JOURNAL   EMBO Mol Med 16 (8), 1817-1839 (2024)
   PUBMED   39009885
  REMARK    GeneRIF: In vitro and in vivo inhibition of the host TRPC4 channel
            attenuates Zika virus infection.
REFERENCE   2  (bases 1 to 7979)
  AUTHORS   Chen,Z., Zhao,Y., Tian,Y., Cao,R. and Shang,D.
  TITLE     Pan-Cancer Analysis of the TRP Family, Especially TRPV4 and TRPC4,
            and Its Expression Correlated with Prognosis, Tumor
            Microenvironment, and Treatment Sensitivity
  JOURNAL   Biomolecules 13 (2), 282 (2023)
   PUBMED   36830651
  REMARK    GeneRIF: Pan-Cancer Analysis of the TRP Family, Especially TRPV4
            and TRPC4, and Its Expression Correlated with Prognosis, Tumor
            Microenvironment, and Treatment Sensitivity.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7979)
  AUTHORS   Gotz,V., Qiao,S., Beck,A. and Boehm,U.
  TITLE     Transient receptor potential (TRP) channel function in the
            reproductive axis
  JOURNAL   Cell Calcium 67, 138-147 (2017)
   PUBMED   28522036
  REMARK    Review article
REFERENCE   4  (bases 1 to 7979)
  AUTHORS   Dietrich,A., Steinritz,D. and Gudermann,T.
  TITLE     Transient receptor potential (TRP) channels as molecular targets in
            lung toxicology and associated diseases
  JOURNAL   Cell Calcium 67, 123-137 (2017)
   PUBMED   28499580
  REMARK    Review article
REFERENCE   5  (bases 1 to 7979)
  AUTHORS   Zeng,B., Yuan,C., Yang,X., Atkin,S.L. and Xu,S.Z.
  TITLE     TRPC channels and their splice variants are essential for promoting
            human ovarian cancer cell proliferation and tumorigenesis
  JOURNAL   Curr Cancer Drug Targets 13 (1), 103-116 (2013)
   PUBMED   22920441
REFERENCE   6  (bases 1 to 7979)
  AUTHORS   von Spiczak,S., Muhle,H., Helbig,I., de Kovel,C.G., Hampe,J.,
            Gaus,V., Koeleman,B.P., Lindhout,D., Schreiber,S., Sander,T. and
            Stephani,U.
  TITLE     Association study of TRPC4 as a candidate gene for generalized
            epilepsy with photosensitivity
  JOURNAL   Neuromolecular Med 12 (3), 292-299 (2010)
   PUBMED   20574736
  REMARK    GeneRIF: Results showed a trend toward association of TRPC4
            variants and photoparoxysmal response/idiopathic generalized
            epilepsies.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 7979)
  AUTHORS   Schaefer,M., Plant,T.D., Stresow,N., Albrecht,N. and Schultz,G.
  TITLE     Functional differences between TRPC4 splice variants
  JOURNAL   J Biol Chem 277 (5), 3752-3759 (2002)
   PUBMED   11713258
REFERENCE   8  (bases 1 to 7979)
  AUTHORS   Mery,L., Magnino,F., Schmidt,K., Krause,K.H. and Dufour,J.F.
  TITLE     Alternative splice variants of hTrp4 differentially interact with
            the C-terminal portion of the inositol 1,4,5-trisphosphate
            receptors
  JOURNAL   FEBS Lett 487 (3), 377-383 (2001)
   PUBMED   11163362
REFERENCE   9  (bases 1 to 7979)
  AUTHORS   McKay,R.R., Szymeczek-Seay,C.L., Lievremont,J.P., Bird,G.S.,
            Zitt,C., Jungling,E., Luckhoff,A. and Putney,J.W. Jr.
  TITLE     Cloning and expression of the human transient receptor potential 4
            (TRP4) gene: localization and functional expression of human TRP4
            and TRP3
  JOURNAL   Biochem J 351 Pt 3 (Pt 3), 735-746 (2000)
   PUBMED   11042129
REFERENCE   10 (bases 1 to 7979)
  AUTHORS   Zhu,X., Jiang,M., Peyton,M., Boulay,G., Hurst,R., Stefani,E. and
            Birnbaumer,L.
  TITLE     trp, a novel mammalian gene family essential for agonist-activated
            capacitative Ca2+ entry
  JOURNAL   Cell 85 (5), 661-671 (1996)
   PUBMED   8646775
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL356495.15, AL354802.15 and
            AL138679.11.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 6, 2019 this sequence version replaced NM_016179.3.
            
            Summary: This gene encodes a member of the canonical subfamily of
            transient receptor potential cation channels. The encoded protein
            forms a non-selective calcium-permeable cation channel that is
            activated by Gq-coupled receptors and tyrosine kinases, and plays a
            role in multiple processes including endothelial permeability,
            vasodilation, neurotransmitter release and cell proliferation.
            Single nucleotide polymorphisms in this gene may be associated with
            generalized epilepsy with photosensitivity. Alternatively spliced
            transcript variants encoding multiple isoforms have been observed
            for this gene. [provided by RefSeq, Aug 2011].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK312524.1, SRR18074969.251394.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2149398 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000379705.8/ ENSP00000369027.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-178               AL356495.15        14744-14921         c
            179-583             AL354802.15        99189-99593         c
            584-1102            AL354802.15        62170-62688         c
            1103-1439           AL354802.15        8232-8568           c
            1440-1579           AL138679.11        94386-94525         c
            1580-1893           AL138679.11        83574-83887         c
            1894-2089           AL138679.11        75246-75441         c
            2090-2284           AL138679.11        71423-71617         c
            2285-2326           AL138679.11        59416-59457         c
            2327-2416           AL138679.11        59198-59287         c
            2417-7979           AL138679.11        52221-57783         c
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q13.3"
     gene            1..7979
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="transient receptor potential cation channel
                     subfamily C member 4"
                     /db_xref="GeneID:7223"
                     /db_xref="HGNC:HGNC:12336"
                     /db_xref="MIM:603651"
     exon            1..178
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            179..583
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     CDS             206..3139
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="isoform alpha is encoded by transcript variant 1;
                     short transient receptor potential channel 4; trp-related
                     protein 4"
                     /codon_start=1
                     /product="short transient receptor potential channel 4
                     isoform alpha"
                     /protein_id="NP_057263.1"
                     /db_xref="CCDS:CCDS9365.1"
                     /db_xref="GeneID:7223"
                     /db_xref="HGNC:HGNC:12336"
                     /db_xref="MIM:603651"
                     /translation="MAQFYYKRNVNAPYRDRIPLRIVRAESELSPSEKAYLNAVEKGD
                     YASVKKSLEEAEIYFKININCIDPLGRTALLIAIENENLELIELLLSFNVYVGDALLH
                     AIRKEVVGAVELLLNHKKPSGEKQVPPILLDKQFSEFTPDITPIILAAHTNNYEIIKL
                     LVQKGVSVPRPHEVRCNCVECVSSSDVDSLRHSRSRLNIYKALASPSLIALSSEDPFL
                     TAFQLSWELQELSKVENEFKSEYEELSRQCKQFAKDLLDQTRSSRELEIILNYRDDNS
                     LIEEQSGNDLARLKLAIKYRQKEFVAQPNCQQLLASRWYDEFPGWRRRHWAVKMVTCF
                     IIGLLFPVFSVCYLIAPKSPLGLFIRKPFIKFICHTASYLTFLFLLLLASQHIDRSDL
                     NRQGPPPTIVEWMILPWVLGFIWGEIKQMWDGGLQDYIHDWWNLMDFVMNSLYLATIS
                     LKIVAFVKYSALNPRESWDMWHPTLVAEALFAIANIFSSLRLISLFTANSHLGPLQIS
                     LGRMLLDILKFLFIYCLVLLAFANGLNQLYFYYEETKGLTCKGIRCEKQNNAFSTLFE
                     TLQSLFWSIFGLINLYVTNVKAQHEFTEFVGATMFGTYNVISLVVLLNMLIAMMNNSY
                     QLIADHADIEWKFARTKLWMSYFEEGGTLPTPFNVIPSPKSLWYLIKWIWTHLCKKKM
                     RRKPESFGTIGRRAADNLRRHHQYQEVMRNLVKRYVAAMIRDAKTEEGLTEENFKELK
                     QDISSFRFEVLGLLRGSKLSTIQSANASKESSNSADSDEKSDSEGNSKDKKKNFSLFD
                     LTTLIHPRSAAIASERHNISNGSALVVQEPPREKQRKVNFVTDIKNFGLFHRRSKQNA
                     AEQNANQIFSVSEEVARQQAAGPLERNIQLESRGLASRGDLSIPGLSEQCVLVDHRER
                     NTDTLGLQVGKRVCPFKSEKVVVEDTVPIIPKEKHAKEEDSSIDYDLNLPDTVTHEDY
                     VTTRL"
     exon            584..1102
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1103..1439
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..1579
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1580..1893
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1894..2089
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2090..2284
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2285..2326
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2327..2416
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2417..7979
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3366..3371
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="hexamer: AATAAA"
     polyA_site      3384
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="major polyA site"
     regulatory      6120..6125
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="hexamer: AATAAA"
     polyA_site      6139
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
     regulatory      6150..6155
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="hexamer: AATAAA"
     polyA_site      6170
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="major polyA site"
     regulatory      6332..6337
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="hexamer: ATTAAA"
     polyA_site      6353
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
     regulatory      7724..7729
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="hexamer: AATATA"
     polyA_site      7742
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
     regulatory      7955..7960
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="hexamer: ATTAAA"
     polyA_site      7979
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
ORIGIN      
        1 agttcggtgc cagctgcgtg ggctccagct tcgatcgttt tccttggaat gctccaaaac
       61 tcagcagcga ctaagggaat tccattggaa tttgccgggc gtgctctcac cccgcacggc
      121 acccgcgccg tcagtcctcg gatcccatca cttcagcccg aagattgcaa ctttgcagag
      181 acgaagaaat agcatggcat gaaacatggc tcagttctat tacaaaagaa atgttaatgc
      241 tccctataga gaccgcatcc ctctaaggat agtaagagca gaatcagaac tctcgccatc
      301 agaaaaagcc tacttgaatg ctgtggaaaa gggagattat gccagtgtca agaaatccct
      361 agaggaagct gaaatttatt ttaaaatcaa tattaattgc attgatcctc tcggaagaac
      421 tgctctcctc attgcaattg aaaatgagaa cttggagctc atcgaactac tcttaagctt
      481 taatgtctat gttggagatg ctctattaca tgctatcaga aaagaagtcg tcggagctgt
      541 tgagctgtta ttgaaccaca aaaaacctag tggagaaaaa caggtgcctc ctatactcct
      601 tgataagcag ttctctgaat tcactccaga cattacacca atcattttgg cagcccatac
      661 aaataattat gagataataa aactcttggt tcagaaagga gtctcagtgc ctcgacccca
      721 cgaggtccgc tgtaactgtg tggaatgcgt gtccagttca gatgtggaca gcctccgtca
      781 ctcacgctcc agactcaaca tctacaaggc cttggccagt ccctctctca ttgcactgtc
      841 aagcgaagat ccttttctca cagcctttca gttaagttgg gaacttcagg aactgagcaa
      901 ggtggaaaat gaattcaagt cggagtatga agagctgtca cggcagtgca aacaatttgc
      961 taaggaccta ctggatcaga cgagaagttc cagagaactg gaaatcattc ttaattaccg
     1021 agatgacaat agtctcatag aagaacaaag tggaaatgat cttgcaagac taaaattggc
     1081 cattaagtac cgtcaaaaag agtttgttgc ccagcccaat tgtcaacagc tgctggcatc
     1141 tcgctggtac gatgagtttc caggctggag gagaagacac tgggcagtga agatggtgac
     1201 atgtttcata ataggacttc tttttcctgt cttctctgtg tgctacctga tagctcccaa
     1261 aagcccactt ggactgttca tcaggaagcc atttatcaag tttatctgcc acacagcctc
     1321 ctatttgact tttttgttcc tgctgctgct tgcctctcag cacatcgaca ggtcagactt
     1381 gaacaggcaa ggtccaccac caaccatcgt cgagtggatg atattaccgt gggtcctggg
     1441 cttcatatgg ggagaaatta aacagatgtg ggatggcgga cttcaggact acatccatga
     1501 ttggtggaat ctaatggact ttgtaatgaa ctccttatat ttagcaacaa tctccttgaa
     1561 aattgttgca tttgtaaagt acagtgccct taatccacga gaatcatggg acatgtggca
     1621 tcccactctg gtggcagagg ctttatttgc tattgcaaac atcttcagtt ctctgcgtct
     1681 gatctcactg tttactgcaa attctcacct gggacctctg caaatatctc tgggaagaat
     1741 gctcctggac attttgaagt ttctattcat atactgcctt gtgttgctag catttgcaaa
     1801 tggcctaaat caattgtact tctattatga agaaacgaaa gggttaacct gcaaaggcat
     1861 aagatgtgaa aagcagaata atgcattttc aacgttattt gagacactgc agtccctgtt
     1921 ttggtcaata tttgggctca tcaatttata tgtgaccaat gtcaaagcac agcatgaatt
     1981 tactgagttt gttggtgcca ccatgtttgg gacatacaat gtcatctctc tggttgttct
     2041 actcaacatg ttaatagcta tgatgaataa ttcttaccaa ctgattgctg accatgcaga
     2101 tatagaatgg aaatttgcac gaacaaagct ttggatgagt tattttgaag aaggaggtac
     2161 tctgcctact cccttcaatg tcatcccgag ccccaagtct ctctggtacc tgatcaaatg
     2221 gatctggaca cacttgtgca agaaaaagat gagaagaaag ccagaaagtt ttggaacaat
     2281 agggaggcga gctgctgata acttgagaag acatcaccaa taccaagaag ttatgaggaa
     2341 cctggtgaag cgatacgttg ctgcaatgat tagagatgct aaaactgaag aaggcctgac
     2401 cgaagagaac tttaaggaac taaagcaaga catttctagt ttccgctttg aagtcctggg
     2461 attactaaga ggaagcaaac tttccacaat acaatctgcg aatgcctcga aggagtcttc
     2521 aaattcggca gactcagatg aaaagagtga tagcgaaggt aatagcaagg acaagaaaaa
     2581 gaatttcagc ctttttgatt taaccaccct gattcatccg agatcagcag caattgcctc
     2641 tgaaagacat aacataagca atggctctgc cctggtggtt caggagccgc ccagggagaa
     2701 gcagagaaaa gtgaattttg tgaccgatat caaaaacttt gggttatttc atagacgatc
     2761 aaaacaaaat gctgctgagc aaaatgcaaa ccaaatcttc tctgtttcag aagaagttgc
     2821 tcgtcaacag gctgcaggac cacttgagag aaatattcaa ctggaatctc gaggattagc
     2881 ttcacggggt gacctgagca ttcccggtct cagtgaacaa tgtgtgttag tagaccatag
     2941 agaaaggaat acggacacac tggggttaca ggtaggaaag agagtgtgtc cattcaagtc
     3001 agagaaggtg gtggtggagg acacggttcc tataatacca aaggagaaac atgcaaaaga
     3061 agaggactct agtatagact atgatctaaa cctcccagac acagtcaccc acgaagatta
     3121 cgtgaccaca agattgtgat acttgaagga ggaagcgttt accatacaca tacgtatttt
     3181 ccgtagtgct ctgggtgggg gaaaatgttt aaattgtatt agcaaatgct aacttacact
     3241 ttatagcgtt tatcagctgt ggcatattac ctgtaacatg tttaaataag gcaaaggcaa
     3301 tcaaaaacct ttttgttttg tagcctgctt ttgctttcac aatttgtctt acaattgttt
     3361 ttgttaataa ataaatgcac cttgtattct tgtactgttg caataaccca cagaaacatt
     3421 ttagctatct ttttcaatta aaacaaatgc aatttatttc atgtggtagt tgactcctaa
     3481 aatagatatt tttatattca aaaaccaatg tggctgaaat attatagctt ttcataattt
     3541 tttagggtct ttagagaggg ttactcacca aggacttttc acctagtcct acagtttggc
     3601 tttaaaacac aaatattacc aaattatttc ttaacgacat gtaaataatt attgtacaca
     3661 aagcaaacaa gtcttcttct catggagtca tatctaaact ccctcaaatt cagaaaaact
     3721 ttgtagtgtt ttaggttgtt ccagggtttc ctttcagtaa gtaaaagaag tgtttgtctt
     3781 aatacatttt gatgactgat gatttttaac gacattttgt caggaaaaaa actcgcccct
     3841 ccagtttttt taattaccta tttcagattc aactgcttat cagtataaat atgagtcttt
     3901 atgaataact gaatttcaaa aatatggcaa gatttctgtt ataaaaaaat aataaaccat
     3961 aatattgtaa gtatcttaaa caagcaagat gattacatac taataatatc caaatgaaaa
     4021 tgaatttatt ctaacaatct aaatctaagt aaagttgaat tccagtattt tcaatcaacg
     4081 caatattgta atcaattcaa aagaagtaca aaaaaactag cagtctcctc ttttcaccag
     4141 taatctttat atatttcttt aaaattaaat cactcaaaaa tcattattaa ttttatcagc
     4201 agatatagat atatagtttt gcctatagga aaaacattct tatttcttgt ttttctttct
     4261 attgtttgca aggtaattta gcttataaca attcactgaa ctattacaag gaaagtgttc
     4321 gcaacttaca taaaattgat actagaaact taaagcttaa attttacatt gcttgtgtct
     4381 atttaagcca ctacgggaag tttcttcacc acataaaatt agcatttatt tcaaatcaat
     4441 agaaaaatgc taagttcatt ttatttcaaa aattactctg cgaacctcag aaattgcatg
     4501 tgatttatca ttggccccaa actgaataat gttatggcac tcagtttctc attaccaaac
     4561 atcaaaaata aggaataaca gcaataacaa tgccagcctc tgttctattc ctatacacct
     4621 gttaacattt atgtactgct ctttacagcc ctctgaagta gctcctgttt ttatccccat
     4681 ttcacatatg atgaaactga gaccagcact aactggctaa ggtaatacag ctcatgatgg
     4741 agctgtgatg tgtccatcag agccattccc ttcaccactg cattgtaact gcctttcaga
     4801 acataaaaag agcaagttat aaaacatgca tatacagggg ttacaattgc cacttctgaa
     4861 gtcctatacc ttgagagaga atgagtttgg tatgcttcat aagagtccct tcttctaaag
     4921 caagagaagt gtgcaaaata taaaatgtgc tgtatttgat tctttagact aaattacatt
     4981 aaagttagta gattttaaaa acatatttat tatgtgatct cagaactcaa tttgaaatac
     5041 tagattttaa ttttcagaaa agccaggagc actaagcccc tgttcactcc taaacctggt
     5101 ttttgggaat ttaggaatgg gccagataga tttggagaaa cacaagccta aatgaatgca
     5161 tttactgttt caagaacacc tttatctcaa gtatattagg actcagagca aatcctgggg
     5221 ctactgaaat actcattttt ttctaaatca tacctacagt agtagagaaa ttgtaggcct
     5281 attgttaata tttgggatat tttctattaa tttctttttg cctctcttgt gcgttgttgt
     5341 tttattttgt tttttcccaa agtaaggaca ctatacataa ccattttaca tactttacca
     5401 gaatttaatt tcaaattctc tctgttccgt caagcaacac caataacagt acaagcaaca
     5461 aaaccttttt tcccccagta gcttatttat atcaggggtt aaggtgctga cattttatag
     5521 ggggacatga tggtaataga ggaatgtatt tggagcaagt aagaccctcc ttttcagtgc
     5581 cctagaaagg atgattctaa caacccccag atgttgctga cagatgtgtt tatgagtatg
     5641 aacaaagtgc tggatattat tagtacccga tgttactaat atcactagaa ttgaatgcta
     5701 ttctaaatac gtagtgacat tatgtagcat taatgaagat gaggagtagg gataaaataa
     5761 gaaaagtcaa acaacagcag gatagcaagc aaagaaatac tgtttctttg aggtttgtaa
     5821 gtgcgtactt cagggtgttc gtttctattc atatttctat ccacagaaat ttctaaatgc
     5881 tacagagaac tttgaaatta ccttcattta cataaataat gtttctcatt tttcgtactc
     5941 tcttaatctt aaaaaatctg tttttcttaa attttgtgtc tgagttaggt gaaggagtgt
     6001 gttgaaaatt cagtttaata gactagcagg taagtttaaa acactttgtt tatcacaatt
     6061 gtattttgga agtcccattt ataagaaagt atgctataaa gatgtacctc attattcgta
     6121 ataaaggttt tccttaaaat ttctgtgcaa ataaatctat attaacttca gtaaggaaat
     6181 tggctatatt ttttaaaatg gggtgaatta ttttataaaa tcatgagtaa tttttcaaag
     6241 tgctaaacat cctcaaaatg tatctgtttc atgaattgaa tatttgtata tcaaattttc
     6301 ttaaattagt tactctattt actggatggc aattaaacta atgcaaaaac atactcactt
     6361 tcttgccttg acgtagacag caacttgaaa tactttagtg atgaaacaaa ttgatgaaaa
     6421 gtcaaggata gtagactcac catgctgttc tcaaagaagc aaagtcaatt ttctagcaaa
     6481 ggtggaggaa acataagtaa caatagcata agaatatatt cttctaacat tcaataatcc
     6541 ttaataactc tgggatttag ctgagtaaat gactatccag tctcacagct ctttattgaa
     6601 gagaggccac caagttttga aatctgtcca ttcttagtcc tcatgcattg tatttttagc
     6661 tgtcttctat ggtgtataca gctgccttcc atgctcagtg tccttaaaac tcaacctagt
     6721 aagaaccatc cattgtggcc ctgtaaatat gcttacaata tatttttctt tctctgtatt
     6781 atctgaaatt ctgacttaaa actaaccata gaatttagaa atttaatatt actggcattc
     6841 ctcttaactt caaaaccatt attctgattt tacaatgtct gaaaagacat tttatttata
     6901 cagtgttatc acatgccttt tttaatttga attttagaat catgttaaat aagttcactg
     6961 tagataactg gggaaagtaa agcccaggga atttatggaa attatctaaa tttacccatg
     7021 tgtttggtgg tcaagttggg gcttaaacaa aaacctcctc atctagatat ttggatgagg
     7081 catcacatta taaatgcctt gaccactgac tttttatttg gggaacattt tgtcttcttt
     7141 gcccttgtag aaaatatagt tgtccaaaca ttcaagttaa aattcagaat taataccata
     7201 ttatcttttg tttttctaca aagttgtgtt aagagaattt ttaaaaagct ggtttcttac
     7261 tgttttcaga aaatacactg ttcattttca ctggaagtgt agattttatg ataacctgta
     7321 tgcatactgg taataataaa atgtattaaa ttatatcaaa gcttctcaac tgaaaaacat
     7381 tagagatgga caatgtacag ttgaactaag attgggacaa tgggaccaag cctggataca
     7441 gtcacaccgt tactaggctg acagtgaaga cgtctctgca agtcaaattc accagtagca
     7501 aaaccgttaa tttcctctag agctgcactg ttcagtaacg tagctcctat actcatacgg
     7561 ctattaatca cttgtaagtg tgacaagtct gaattaagat gtgctgtaag tgtgaaacac
     7621 acaccagatt tctaggacag tatgaaaata atgtaaacta gctcataaat attttatgtt
     7681 ggctacatat tgaactgata gtatttatga agtattgatt taaaatatac taagtaaatg
     7741 agtttgatgt ttctttttcc ttttacaatg tggctactag aaaactgaaa aatacgtatg
     7801 tggcttgcat ttgtgccttg caatacattt ctattggaca gtgctgctca ggaacatttg
     7861 tatttgtttc agtcagctca tgtatctcca tcatctgaat tgcaaaggta gaaaattcta
     7921 tctttaatta ctcaaaaaag ttttgtgtag ctctattaaa gtttgcagtc tctccttga
//
