LOCUS       NM_003306               7994 bp    mRNA    linear   PRI 12-DEC-2024
DEFINITION  Homo sapiens transient receptor potential cation channel subfamily
            C member 4 (TRPC4), transcript variant 5, mRNA.
ACCESSION   NM_003306
VERSION     NM_003306.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7994)
  AUTHORS   Chen,X., Yan,Y., Liu,Z., Yang,S., Li,W., Wang,Z., Wang,M., Guo,J.,
            Li,Z., Zhu,W., Yang,J., Yin,J., Dai,Q., Li,Y., Wang,C., Zhao,L.,
            Yang,X., Guo,X., Leng,L., Xu,J., Obukhov,A.G., Cao,R. and Zhong,W.
  TITLE     In vitro and in vivo inhibition of the host TRPC4 channel
            attenuates Zika virus infection
  JOURNAL   EMBO Mol Med 16 (8), 1817-1839 (2024)
   PUBMED   39009885
  REMARK    GeneRIF: In vitro and in vivo inhibition of the host TRPC4 channel
            attenuates Zika virus infection.
REFERENCE   2  (bases 1 to 7994)
  AUTHORS   Chen,Z., Zhao,Y., Tian,Y., Cao,R. and Shang,D.
  TITLE     Pan-Cancer Analysis of the TRP Family, Especially TRPV4 and TRPC4,
            and Its Expression Correlated with Prognosis, Tumor
            Microenvironment, and Treatment Sensitivity
  JOURNAL   Biomolecules 13 (2), 282 (2023)
   PUBMED   36830651
  REMARK    GeneRIF: Pan-Cancer Analysis of the TRP Family, Especially TRPV4
            and TRPC4, and Its Expression Correlated with Prognosis, Tumor
            Microenvironment, and Treatment Sensitivity.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7994)
  AUTHORS   Gotz,V., Qiao,S., Beck,A. and Boehm,U.
  TITLE     Transient receptor potential (TRP) channel function in the
            reproductive axis
  JOURNAL   Cell Calcium 67, 138-147 (2017)
   PUBMED   28522036
  REMARK    Review article
REFERENCE   4  (bases 1 to 7994)
  AUTHORS   Dietrich,A., Steinritz,D. and Gudermann,T.
  TITLE     Transient receptor potential (TRP) channels as molecular targets in
            lung toxicology and associated diseases
  JOURNAL   Cell Calcium 67, 123-137 (2017)
   PUBMED   28499580
  REMARK    Review article
REFERENCE   5  (bases 1 to 7994)
  AUTHORS   Zeng,B., Yuan,C., Yang,X., Atkin,S.L. and Xu,S.Z.
  TITLE     TRPC channels and their splice variants are essential for promoting
            human ovarian cancer cell proliferation and tumorigenesis
  JOURNAL   Curr Cancer Drug Targets 13 (1), 103-116 (2013)
   PUBMED   22920441
REFERENCE   6  (bases 1 to 7994)
  AUTHORS   von Spiczak,S., Muhle,H., Helbig,I., de Kovel,C.G., Hampe,J.,
            Gaus,V., Koeleman,B.P., Lindhout,D., Schreiber,S., Sander,T. and
            Stephani,U.
  TITLE     Association study of TRPC4 as a candidate gene for generalized
            epilepsy with photosensitivity
  JOURNAL   Neuromolecular Med 12 (3), 292-299 (2010)
   PUBMED   20574736
  REMARK    GeneRIF: Results showed a trend toward association of TRPC4
            variants and photoparoxysmal response/idiopathic generalized
            epilepsies.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 7994)
  AUTHORS   Schaefer,M., Plant,T.D., Stresow,N., Albrecht,N. and Schultz,G.
  TITLE     Functional differences between TRPC4 splice variants
  JOURNAL   J Biol Chem 277 (5), 3752-3759 (2002)
   PUBMED   11713258
REFERENCE   8  (bases 1 to 7994)
  AUTHORS   Mery,L., Magnino,F., Schmidt,K., Krause,K.H. and Dufour,J.F.
  TITLE     Alternative splice variants of hTrp4 differentially interact with
            the C-terminal portion of the inositol 1,4,5-trisphosphate
            receptors
  JOURNAL   FEBS Lett 487 (3), 377-383 (2001)
   PUBMED   11163362
REFERENCE   9  (bases 1 to 7994)
  AUTHORS   McKay,R.R., Szymeczek-Seay,C.L., Lievremont,J.P., Bird,G.S.,
            Zitt,C., Jungling,E., Luckhoff,A. and Putney,J.W. Jr.
  TITLE     Cloning and expression of the human transient receptor potential 4
            (TRP4) gene: localization and functional expression of human TRP4
            and TRP3
  JOURNAL   Biochem J 351 Pt 3 (Pt 3), 735-746 (2000)
   PUBMED   11042129
REFERENCE   10 (bases 1 to 7994)
  AUTHORS   Zhu,X., Jiang,M., Peyton,M., Boulay,G., Hurst,R., Stefani,E. and
            Birnbaumer,L.
  TITLE     trp, a novel mammalian gene family essential for agonist-activated
            capacitative Ca2+ entry
  JOURNAL   Cell 85 (5), 661-671 (1996)
   PUBMED   8646775
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL356495.15, AL354802.15 and
            AL138679.11.
            
            On Aug 6, 2019 this sequence version replaced NM_003306.2.
            
            Summary: This gene encodes a member of the canonical subfamily of
            transient receptor potential cation channels. The encoded protein
            forms a non-selective calcium-permeable cation channel that is
            activated by Gq-coupled receptors and tyrosine kinases, and plays a
            role in multiple processes including endothelial permeability,
            vasodilation, neurotransmitter release and cell proliferation.
            Single nucleotide polymorphisms in this gene may be associated with
            generalized epilepsy with photosensitivity. Alternatively spliced
            transcript variants encoding multiple isoforms have been observed
            for this gene. [provided by RefSeq, Aug 2011].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.2749475.1,
                                           SRR14372080.3075732.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA2142853
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-178               AL356495.15        14744-14921         c
            179-583             AL354802.15        99189-99593         c
            584-1102            AL354802.15        62170-62688         c
            1103-1439           AL354802.15        8232-8568           c
            1440-1579           AL138679.11        94386-94525         c
            1580-1893           AL138679.11        83574-83887         c
            1894-2089           AL138679.11        75246-75441         c
            2090-2299           AL138679.11        71408-71617         c
            2300-2341           AL138679.11        59416-59457         c
            2342-2431           AL138679.11        59198-59287         c
            2432-7994           AL138679.11        52221-57783         c
FEATURES             Location/Qualifiers
     source          1..7994
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q13.3"
     gene            1..7994
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="transient receptor potential cation channel
                     subfamily C member 4"
                     /db_xref="GeneID:7223"
                     /db_xref="HGNC:HGNC:12336"
                     /db_xref="MIM:603651"
     exon            1..178
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            179..583
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     CDS             206..3154
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="isoform epsilon is encoded by transcript variant 5;
                     short transient receptor potential channel 4; trp-related
                     protein 4"
                     /codon_start=1
                     /product="short transient receptor potential channel 4
                     isoform epsilon"
                     /protein_id="NP_003297.1"
                     /db_xref="CCDS:CCDS45037.1"
                     /db_xref="GeneID:7223"
                     /db_xref="HGNC:HGNC:12336"
                     /db_xref="MIM:603651"
                     /translation="MAQFYYKRNVNAPYRDRIPLRIVRAESELSPSEKAYLNAVEKGD
                     YASVKKSLEEAEIYFKININCIDPLGRTALLIAIENENLELIELLLSFNVYVGDALLH
                     AIRKEVVGAVELLLNHKKPSGEKQVPPILLDKQFSEFTPDITPIILAAHTNNYEIIKL
                     LVQKGVSVPRPHEVRCNCVECVSSSDVDSLRHSRSRLNIYKALASPSLIALSSEDPFL
                     TAFQLSWELQELSKVENEFKSEYEELSRQCKQFAKDLLDQTRSSRELEIILNYRDDNS
                     LIEEQSGNDLARLKLAIKYRQKEFVAQPNCQQLLASRWYDEFPGWRRRHWAVKMVTCF
                     IIGLLFPVFSVCYLIAPKSPLGLFIRKPFIKFICHTASYLTFLFLLLLASQHIDRSDL
                     NRQGPPPTIVEWMILPWVLGFIWGEIKQMWDGGLQDYIHDWWNLMDFVMNSLYLATIS
                     LKIVAFVKYSALNPRESWDMWHPTLVAEALFAIANIFSSLRLISLFTANSHLGPLQIS
                     LGRMLLDILKFLFIYCLVLLAFANGLNQLYFYYEETKGLTCKGIRCEKQNNAFSTLFE
                     TLQSLFWSIFGLINLYVTNVKAQHEFTEFVGATMFGTYNVISLVVLLNMLIAMMNNSY
                     QLIADHADIEWKFARTKLWMSYFEEGGTLPTPFNVIPSPKSLWYLIKWIWTHLCKKKM
                     RRKPESFGTIGVRTQHRRAADNLRRHHQYQEVMRNLVKRYVAAMIRDAKTEEGLTEEN
                     FKELKQDISSFRFEVLGLLRGSKLSTIQSANASKESSNSADSDEKSDSEGNSKDKKKN
                     FSLFDLTTLIHPRSAAIASERHNISNGSALVVQEPPREKQRKVNFVTDIKNFGLFHRR
                     SKQNAAEQNANQIFSVSEEVARQQAAGPLERNIQLESRGLASRGDLSIPGLSEQCVLV
                     DHRERNTDTLGLQVGKRVCPFKSEKVVVEDTVPIIPKEKHAKEEDSSIDYDLNLPDTV
                     THEDYVTTRL"
     exon            584..1102
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1103..1439
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..1579
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1580..1893
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1894..2089
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2090..2299
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2300..2341
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2342..2431
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2432..7994
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3381..3386
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="hexamer: AATAAA"
     polyA_site      3399
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="major polyA site"
     regulatory      6135..6140
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="hexamer: AATAAA"
     polyA_site      6154
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
     regulatory      6165..6170
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="hexamer: AATAAA"
     polyA_site      6185
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="major polyA site"
     regulatory      6347..6352
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="hexamer: ATTAAA"
     polyA_site      6368
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
     regulatory      7739..7744
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="hexamer: AATATA"
     polyA_site      7757
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
     regulatory      7970..7975
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
                     /note="hexamer: ATTAAA"
     polyA_site      7994
                     /gene="TRPC4"
                     /gene_synonym="HTRP-4; HTRP4; TRP4"
ORIGIN      
        1 agttcggtgc cagctgcgtg ggctccagct tcgatcgttt tccttggaat gctccaaaac
       61 tcagcagcga ctaagggaat tccattggaa tttgccgggc gtgctctcac cccgcacggc
      121 acccgcgccg tcagtcctcg gatcccatca cttcagcccg aagattgcaa ctttgcagag
      181 acgaagaaat agcatggcat gaaacatggc tcagttctat tacaaaagaa atgttaatgc
      241 tccctataga gaccgcatcc ctctaaggat agtaagagca gaatcagaac tctcgccatc
      301 agaaaaagcc tacttgaatg ctgtggaaaa gggagattat gccagtgtca agaaatccct
      361 agaggaagct gaaatttatt ttaaaatcaa tattaattgc attgatcctc tcggaagaac
      421 tgctctcctc attgcaattg aaaatgagaa cttggagctc atcgaactac tcttaagctt
      481 taatgtctat gttggagatg ctctattaca tgctatcaga aaagaagtcg tcggagctgt
      541 tgagctgtta ttgaaccaca aaaaacctag tggagaaaaa caggtgcctc ctatactcct
      601 tgataagcag ttctctgaat tcactccaga cattacacca atcattttgg cagcccatac
      661 aaataattat gagataataa aactcttggt tcagaaagga gtctcagtgc ctcgacccca
      721 cgaggtccgc tgtaactgtg tggaatgcgt gtccagttca gatgtggaca gcctccgtca
      781 ctcacgctcc agactcaaca tctacaaggc cttggccagt ccctctctca ttgcactgtc
      841 aagcgaagat ccttttctca cagcctttca gttaagttgg gaacttcagg aactgagcaa
      901 ggtggaaaat gaattcaagt cggagtatga agagctgtca cggcagtgca aacaatttgc
      961 taaggaccta ctggatcaga cgagaagttc cagagaactg gaaatcattc ttaattaccg
     1021 agatgacaat agtctcatag aagaacaaag tggaaatgat cttgcaagac taaaattggc
     1081 cattaagtac cgtcaaaaag agtttgttgc ccagcccaat tgtcaacagc tgctggcatc
     1141 tcgctggtac gatgagtttc caggctggag gagaagacac tgggcagtga agatggtgac
     1201 atgtttcata ataggacttc tttttcctgt cttctctgtg tgctacctga tagctcccaa
     1261 aagcccactt ggactgttca tcaggaagcc atttatcaag tttatctgcc acacagcctc
     1321 ctatttgact tttttgttcc tgctgctgct tgcctctcag cacatcgaca ggtcagactt
     1381 gaacaggcaa ggtccaccac caaccatcgt cgagtggatg atattaccgt gggtcctggg
     1441 cttcatatgg ggagaaatta aacagatgtg ggatggcgga cttcaggact acatccatga
     1501 ttggtggaat ctaatggact ttgtaatgaa ctccttatat ttagcaacaa tctccttgaa
     1561 aattgttgca tttgtaaagt acagtgccct taatccacga gaatcatggg acatgtggca
     1621 tcccactctg gtggcagagg ctttatttgc tattgcaaac atcttcagtt ctctgcgtct
     1681 gatctcactg tttactgcaa attctcacct gggacctctg caaatatctc tgggaagaat
     1741 gctcctggac attttgaagt ttctattcat atactgcctt gtgttgctag catttgcaaa
     1801 tggcctaaat caattgtact tctattatga agaaacgaaa gggttaacct gcaaaggcat
     1861 aagatgtgaa aagcagaata atgcattttc aacgttattt gagacactgc agtccctgtt
     1921 ttggtcaata tttgggctca tcaatttata tgtgaccaat gtcaaagcac agcatgaatt
     1981 tactgagttt gttggtgcca ccatgtttgg gacatacaat gtcatctctc tggttgttct
     2041 actcaacatg ttaatagcta tgatgaataa ttcttaccaa ctgattgctg accatgcaga
     2101 tatagaatgg aaatttgcac gaacaaagct ttggatgagt tattttgaag aaggaggtac
     2161 tctgcctact cccttcaatg tcatcccgag ccccaagtct ctctggtacc tgatcaaatg
     2221 gatctggaca cacttgtgca agaaaaagat gagaagaaag ccagaaagtt ttggaacaat
     2281 aggggtaaga acacagcata ggcgagctgc tgataacttg agaagacatc accaatacca
     2341 agaagttatg aggaacctgg tgaagcgata cgttgctgca atgattagag atgctaaaac
     2401 tgaagaaggc ctgaccgaag agaactttaa ggaactaaag caagacattt ctagtttccg
     2461 ctttgaagtc ctgggattac taagaggaag caaactttcc acaatacaat ctgcgaatgc
     2521 ctcgaaggag tcttcaaatt cggcagactc agatgaaaag agtgatagcg aaggtaatag
     2581 caaggacaag aaaaagaatt tcagcctttt tgatttaacc accctgattc atccgagatc
     2641 agcagcaatt gcctctgaaa gacataacat aagcaatggc tctgccctgg tggttcagga
     2701 gccgcccagg gagaagcaga gaaaagtgaa ttttgtgacc gatatcaaaa actttgggtt
     2761 atttcataga cgatcaaaac aaaatgctgc tgagcaaaat gcaaaccaaa tcttctctgt
     2821 ttcagaagaa gttgctcgtc aacaggctgc aggaccactt gagagaaata ttcaactgga
     2881 atctcgagga ttagcttcac ggggtgacct gagcattccc ggtctcagtg aacaatgtgt
     2941 gttagtagac catagagaaa ggaatacgga cacactgggg ttacaggtag gaaagagagt
     3001 gtgtccattc aagtcagaga aggtggtggt ggaggacacg gttcctataa taccaaagga
     3061 gaaacatgca aaagaagagg actctagtat agactatgat ctaaacctcc cagacacagt
     3121 cacccacgaa gattacgtga ccacaagatt gtgatacttg aaggaggaag cgtttaccat
     3181 acacatacgt attttccgta gtgctctggg tgggggaaaa tgtttaaatt gtattagcaa
     3241 atgctaactt acactttata gcgtttatca gctgtggcat attacctgta acatgtttaa
     3301 ataaggcaaa ggcaatcaaa aacctttttg ttttgtagcc tgcttttgct ttcacaattt
     3361 gtcttacaat tgtttttgtt aataaataaa tgcaccttgt attcttgtac tgttgcaata
     3421 acccacagaa acattttagc tatctttttc aattaaaaca aatgcaattt atttcatgtg
     3481 gtagttgact cctaaaatag atatttttat attcaaaaac caatgtggct gaaatattat
     3541 agcttttcat aattttttag ggtctttaga gagggttact caccaaggac ttttcaccta
     3601 gtcctacagt ttggctttaa aacacaaata ttaccaaatt atttcttaac gacatgtaaa
     3661 taattattgt acacaaagca aacaagtctt cttctcatgg agtcatatct aaactccctc
     3721 aaattcagaa aaactttgta gtgttttagg ttgttccagg gtttcctttc agtaagtaaa
     3781 agaagtgttt gtcttaatac attttgatga ctgatgattt ttaacgacat tttgtcagga
     3841 aaaaaactcg cccctccagt ttttttaatt acctatttca gattcaactg cttatcagta
     3901 taaatatgag tctttatgaa taactgaatt tcaaaaatat ggcaagattt ctgttataaa
     3961 aaaataataa accataatat tgtaagtatc ttaaacaagc aagatgatta catactaata
     4021 atatccaaat gaaaatgaat ttattctaac aatctaaatc taagtaaagt tgaattccag
     4081 tattttcaat caacgcaata ttgtaatcaa ttcaaaagaa gtacaaaaaa actagcagtc
     4141 tcctcttttc accagtaatc tttatatatt tctttaaaat taaatcactc aaaaatcatt
     4201 attaatttta tcagcagata tagatatata gttttgccta taggaaaaac attcttattt
     4261 cttgtttttc tttctattgt ttgcaaggta atttagctta taacaattca ctgaactatt
     4321 acaaggaaag tgttcgcaac ttacataaaa ttgatactag aaacttaaag cttaaatttt
     4381 acattgcttg tgtctattta agccactacg ggaagtttct tcaccacata aaattagcat
     4441 ttatttcaaa tcaatagaaa aatgctaagt tcattttatt tcaaaaatta ctctgcgaac
     4501 ctcagaaatt gcatgtgatt tatcattggc cccaaactga ataatgttat ggcactcagt
     4561 ttctcattac caaacatcaa aaataaggaa taacagcaat aacaatgcca gcctctgttc
     4621 tattcctata cacctgttaa catttatgta ctgctcttta cagccctctg aagtagctcc
     4681 tgtttttatc cccatttcac atatgatgaa actgagacca gcactaactg gctaaggtaa
     4741 tacagctcat gatggagctg tgatgtgtcc atcagagcca ttcccttcac cactgcattg
     4801 taactgcctt tcagaacata aaaagagcaa gttataaaac atgcatatac aggggttaca
     4861 attgccactt ctgaagtcct ataccttgag agagaatgag tttggtatgc ttcataagag
     4921 tcccttcttc taaagcaaga gaagtgtgca aaatataaaa tgtgctgtat ttgattcttt
     4981 agactaaatt acattaaagt tagtagattt taaaaacata tttattatgt gatctcagaa
     5041 ctcaatttga aatactagat tttaattttc agaaaagcca ggagcactaa gcccctgttc
     5101 actcctaaac ctggtttttg ggaatttagg aatgggccag atagatttgg agaaacacaa
     5161 gcctaaatga atgcatttac tgtttcaaga acacctttat ctcaagtata ttaggactca
     5221 gagcaaatcc tggggctact gaaatactca tttttttcta aatcatacct acagtagtag
     5281 agaaattgta ggcctattgt taatatttgg gatattttct attaatttct ttttgcctct
     5341 cttgtgcgtt gttgttttat tttgtttttt cccaaagtaa ggacactata cataaccatt
     5401 ttacatactt taccagaatt taatttcaaa ttctctctgt tccgtcaagc aacaccaata
     5461 acagtacaag caacaaaacc ttttttcccc cagtagctta tttatatcag gggttaaggt
     5521 gctgacattt tataggggga catgatggta atagaggaat gtatttggag caagtaagac
     5581 cctccttttc agtgccctag aaaggatgat tctaacaacc cccagatgtt gctgacagat
     5641 gtgtttatga gtatgaacaa agtgctggat attattagta cccgatgtta ctaatatcac
     5701 tagaattgaa tgctattcta aatacgtagt gacattatgt agcattaatg aagatgagga
     5761 gtagggataa aataagaaaa gtcaaacaac agcaggatag caagcaaaga aatactgttt
     5821 ctttgaggtt tgtaagtgcg tacttcaggg tgttcgtttc tattcatatt tctatccaca
     5881 gaaatttcta aatgctacag agaactttga aattaccttc atttacataa ataatgtttc
     5941 tcatttttcg tactctctta atcttaaaaa atctgttttt cttaaatttt gtgtctgagt
     6001 taggtgaagg agtgtgttga aaattcagtt taatagacta gcaggtaagt ttaaaacact
     6061 ttgtttatca caattgtatt ttggaagtcc catttataag aaagtatgct ataaagatgt
     6121 acctcattat tcgtaataaa ggttttcctt aaaatttctg tgcaaataaa tctatattaa
     6181 cttcagtaag gaaattggct atatttttta aaatggggtg aattatttta taaaatcatg
     6241 agtaattttt caaagtgcta aacatcctca aaatgtatct gtttcatgaa ttgaatattt
     6301 gtatatcaaa ttttcttaaa ttagttactc tatttactgg atggcaatta aactaatgca
     6361 aaaacatact cactttcttg ccttgacgta gacagcaact tgaaatactt tagtgatgaa
     6421 acaaattgat gaaaagtcaa ggatagtaga ctcaccatgc tgttctcaaa gaagcaaagt
     6481 caattttcta gcaaaggtgg aggaaacata agtaacaata gcataagaat atattcttct
     6541 aacattcaat aatccttaat aactctggga tttagctgag taaatgacta tccagtctca
     6601 cagctcttta ttgaagagag gccaccaagt tttgaaatct gtccattctt agtcctcatg
     6661 cattgtattt ttagctgtct tctatggtgt atacagctgc cttccatgct cagtgtcctt
     6721 aaaactcaac ctagtaagaa ccatccattg tggccctgta aatatgctta caatatattt
     6781 ttctttctct gtattatctg aaattctgac ttaaaactaa ccatagaatt tagaaattta
     6841 atattactgg cattcctctt aacttcaaaa ccattattct gattttacaa tgtctgaaaa
     6901 gacattttat ttatacagtg ttatcacatg ccttttttaa tttgaatttt agaatcatgt
     6961 taaataagtt cactgtagat aactggggaa agtaaagccc agggaattta tggaaattat
     7021 ctaaatttac ccatgtgttt ggtggtcaag ttggggctta aacaaaaacc tcctcatcta
     7081 gatatttgga tgaggcatca cattataaat gccttgacca ctgacttttt atttggggaa
     7141 cattttgtct tctttgccct tgtagaaaat atagttgtcc aaacattcaa gttaaaattc
     7201 agaattaata ccatattatc ttttgttttt ctacaaagtt gtgttaagag aatttttaaa
     7261 aagctggttt cttactgttt tcagaaaata cactgttcat tttcactgga agtgtagatt
     7321 ttatgataac ctgtatgcat actggtaata ataaaatgta ttaaattata tcaaagcttc
     7381 tcaactgaaa aacattagag atggacaatg tacagttgaa ctaagattgg gacaatggga
     7441 ccaagcctgg atacagtcac accgttacta ggctgacagt gaagacgtct ctgcaagtca
     7501 aattcaccag tagcaaaacc gttaatttcc tctagagctg cactgttcag taacgtagct
     7561 cctatactca tacggctatt aatcacttgt aagtgtgaca agtctgaatt aagatgtgct
     7621 gtaagtgtga aacacacacc agatttctag gacagtatga aaataatgta aactagctca
     7681 taaatatttt atgttggcta catattgaac tgatagtatt tatgaagtat tgatttaaaa
     7741 tatactaagt aaatgagttt gatgtttctt tttcctttta caatgtggct actagaaaac
     7801 tgaaaaatac gtatgtggct tgcatttgtg ccttgcaata catttctatt ggacagtgct
     7861 gctcaggaac atttgtattt gtttcagtca gctcatgtat ctccatcatc tgaattgcaa
     7921 aggtagaaaa ttctatcttt aattactcaa aaaagttttg tgtagctcta ttaaagtttg
     7981 cagtctctcc ttga
//
