LOCUS       NM_017846               1799 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens tRNA selenocysteine 1 associated protein 1 (TRNAU1AP),
            transcript variant 1, mRNA.
ACCESSION   NM_017846
VERSION     NM_017846.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Hu,X., Luo,J., Lai,H., Li,M., Zheng,X., Nie,T., Li,F. and Li,H.
  TITLE     Knockdown of Trnau1ap inhibits the proliferation and migration of
            NIH3T3, JEG-3 and Bewo cells via the PI3K/Akt signaling pathway
  JOURNAL   Biochem Biophys Res Commun 503 (2), 521-527 (2018)
   PUBMED   29758194
  REMARK    GeneRIF: Knockdown of Trnau1ap reduced cell migration and
            proliferation. Knockdown of Trnau1ap blocked the PI3K/Akt signaling
            pathway.
REFERENCE   3  (bases 1 to 1799)
  AUTHORS   Oudouhou,F., Casu,B., Dopgwa Puemi,A.S., Sygusch,J. and Baron,C.
  TITLE     Analysis of Novel Interactions between Components of the
            Selenocysteine Biosynthesis Pathway, SEPHS1, SEPHS2, SEPSECS, and
            SECp43
  JOURNAL   Biochemistry 56 (17), 2261-2270 (2017)
   PUBMED   28414460
  REMARK    GeneRIF: Data confirm interactions among components of the early
            steps of the selenocysteine biosynthesis pathway (SEPSECS, SECP43,
            SEPHS1, and SEPHS2); SECP43, which interacts with SEPSECS and
            SEPHS1, is a globular protein that forms oligomers in vivo.
REFERENCE   4  (bases 1 to 1799)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   5  (bases 1 to 1799)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   6  (bases 1 to 1799)
  AUTHORS   Small-Howard,A., Morozova,N., Stoytcheva,Z., Forry,E.P.,
            Mansell,J.B., Harney,J.W., Carlson,B.A., Xu,X.M., Hatfield,D.L. and
            Berry,M.J.
  TITLE     Supramolecular complexes mediate selenocysteine incorporation in
            vivo
  JOURNAL   Mol Cell Biol 26 (6), 2337-2346 (2006)
   PUBMED   16508009
  REMARK    GeneRIF: our studies delineating the multiple, coordinated
            protein-nucleic acid interactions between SECp43 and the
            selenoprotein cotranslational factors.
REFERENCE   7  (bases 1 to 1799)
  AUTHORS   Xu,X.M., Mix,H., Carlson,B.A., Grabowski,P.J., Gladyshev,V.N.,
            Berry,M.J. and Hatfield,D.L.
  TITLE     Evidence for direct roles of two additional factors, SECp43 and
            soluble liver antigen, in the selenoprotein synthesis machinery
  JOURNAL   J Biol Chem 280 (50), 41568-41575 (2005)
   PUBMED   16230358
REFERENCE   8  (bases 1 to 1799)
  AUTHORS   Ding,F. and Grabowski,P.J.
  TITLE     Identification of a protein component of a mammalian tRNA(Sec)
            complex implicated in the decoding of UGA as selenocysteine
  JOURNAL   RNA 5 (12), 1561-1569 (1999)
   PUBMED   10606267
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BI602122.1, BC000680.2 and BQ014404.1.
            
            On Nov 22, 2018 this sequence version replaced NM_017846.4.
            
            Transcript Variant: This variant (1) represents the protein-coding
            transcript.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163657.555478.1,
                                           SRR1803613.76686.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373830.4/ ENSP00000362936.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 BI602122.1         74-81
            9-1793              BC000680.2         1-1785
            1794-1799           BQ014404.1         18-23               c
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p35.3"
     gene            1..1799
                     /gene="TRNAU1AP"
                     /gene_synonym="PRO1902; SECP43; TRSPAP1"
                     /note="tRNA selenocysteine 1 associated protein 1"
                     /db_xref="GeneID:54952"
                     /db_xref="HGNC:HGNC:30813"
                     /db_xref="MIM:619597"
     exon            1..53
                     /gene="TRNAU1AP"
                     /gene_synonym="PRO1902; SECP43; TRSPAP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..890
                     /gene="TRNAU1AP"
                     /gene_synonym="PRO1902; SECP43; TRSPAP1"
                     /note="tRNA selenocysteine associated protein (SECP43);
                     tRNA selenocysteine-associated protein 1"
                     /codon_start=1
                     /product="tRNA selenocysteine 1-associated protein 1"
                     /protein_id="NP_060316.1"
                     /db_xref="CCDS:CCDS324.1"
                     /db_xref="GeneID:54952"
                     /db_xref="HGNC:HGNC:30813"
                     /db_xref="MIM:619597"
                     /translation="MAASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIP
                     AGYCFVEFADLATAEKCLHKINGKPLPGATPAKRFKLNYATYGKQPDNSPEYSLFVGD
                     LTPDVDDGMLYEFFVKVYPSCRGGKVVLDQTGVSKGYGFVKFTDELEQKRALTECQGA
                     VGLGSKPVRLSVAIPKASRVKPVEYSQMYSYSYNQYYQQYQNYYAQWGYDQNTGSYSY
                     SYPQYGYTQSTMQTYEEVGDDALEDPMPQLDVTEANKEFMEQSEELYDALMDCHWQPL
                     DTVSSEIPAMM"
     exon            54..151
                     /gene="TRNAU1AP"
                     /gene_synonym="PRO1902; SECP43; TRSPAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            152..251
                     /gene="TRNAU1AP"
                     /gene_synonym="PRO1902; SECP43; TRSPAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            252..304
                     /gene="TRNAU1AP"
                     /gene_synonym="PRO1902; SECP43; TRSPAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            305..436
                     /gene="TRNAU1AP"
                     /gene_synonym="PRO1902; SECP43; TRSPAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            437..556
                     /gene="TRNAU1AP"
                     /gene_synonym="PRO1902; SECP43; TRSPAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            557..719
                     /gene="TRNAU1AP"
                     /gene_synonym="PRO1902; SECP43; TRSPAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            720..753
                     /gene="TRNAU1AP"
                     /gene_synonym="PRO1902; SECP43; TRSPAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            754..1799
                     /gene="TRNAU1AP"
                     /gene_synonym="PRO1902; SECP43; TRSPAP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      991..996
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRNAU1AP"
                     /gene_synonym="PRO1902; SECP43; TRSPAP1"
                     /note="hexamer: AATAAT"
     polyA_site      1018
                     /gene="TRNAU1AP"
                     /gene_synonym="PRO1902; SECP43; TRSPAP1"
                     /note="major polyA site"
     regulatory      1776..1781
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRNAU1AP"
                     /gene_synonym="PRO1902; SECP43; TRSPAP1"
                     /note="hexamer: AATACA"
     polyA_site      1799
                     /gene="TRNAU1AP"
                     /gene_synonym="PRO1902; SECP43; TRSPAP1"
ORIGIN      
        1 gcaaagcccc accccggtgc gcgggtatgg cggccagcct gtggatgggc gacctggaac
       61 cctacatgga tgagaacttc atctccagag cctttgccac catgggggag accgtaatga
      121 gcgtcaaaat tatccgaaac cgcctcactg ggatcccagc tggctactgc tttgtagaat
      181 ttgcagattt ggccacagct gagaagtgtt tgcataaaat taatgggaaa ccccttccag
      241 gagccacacc tgcgaaacgt tttaaactga actatgccac ttacgggaaa caaccagata
      301 acagccctga gtattccctc tttgtggggg acctgacccc ggacgtggat gatggcatgc
      361 tgtatgaatt cttcgtcaaa gtctacccct cctgtcgggg aggcaaggtg gttttggacc
      421 agacaggcgt gtctaagggt tatggttttg tgaaattcac agatgaactg gaacagaagc
      481 gagccctgac ggagtgccag ggagcagtgg gactggggtc taagcctgtg cggctgagcg
      541 tggcaatccc taaagcgagc cgtgtaaagc cagtggaata tagtcagatg tacagttata
      601 gctacaacca gtattatcag cagtaccaga actactatgc tcagtggggc tatgaccaga
      661 acacaggcag ctacagctac agttaccccc agtatggcta tacccagagc accatgcaga
      721 catatgaaga agttggagat gatgcattgg aagaccccat gccacagctg gatgtgactg
      781 aggccaacaa ggagttcatg gaacagagtg aggagctgta tgacgctctg atggactgtc
      841 actggcagcc cctggacaca gtgtcttcag agatccctgc catgatgtag ccaggccaaa
      901 ggacaagcca ggttgcatga tgtgagggag atgagagact cctttttaaa aattgtgaaa
      961 cctttttgga aatatgattt gtaagatttt aataatgact gtttttggag atcatgaatg
     1021 tttctacaac actgctgcat tcatttgacc atttgagttt gaagaccaag ggaacaactt
     1081 ttaaacaagg ttcaaattgg tttccttcac aggaatcctt tgtccaggta gttatccaca
     1141 taggacagtt tgggattatc caaaactggg atgagcagct tgggataatt cacacactaa
     1201 agcctgagtt caaggtttct cagttgacag gtaggtatgt aaatcagggt tctctggagt
     1261 atttgtagga cctgccatat ttgagtccta acttggtgca agtctggcag ctaggtagaa
     1321 aaggcactct agcaggtgtg gtaggagcac gccccagaag gtagaatgtg gtggggtgtg
     1381 atggaacagg ccagttggcc taagaattag tacttgctct aataatgggt ttcatctatt
     1441 ttcatgagtt gcagcatctg catatacaag gcctggatca ctttaggatc aatattttga
     1501 cacgggagaa ggcagctcag aaaggattaa ggaagatagc tcaaagccaa agcagaccct
     1561 ccgtgtgaac tttatactgc tcacttccgt gtttcccaag taaatgggga aactgtccaa
     1621 ccttgtcatg tgttgggact tactgcttga ggcaaagcat tcatcctaca tcataaaaaa
     1681 tctccaaaga tctctttaag ggctgggcat ggtggctcac tttgggagat ggattgcttg
     1741 agctcgggaa gagaccagtt cggtctctac aaaaaaatac aaacacaaat atactttta
//
