LOCUS       NM_024917               3547 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens tRNA methyltransferase 2 homolog B (TRMT2B),
            transcript variant 1, mRNA.
ACCESSION   NM_024917
VERSION     NM_024917.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Liu,Y., He,X., Yuan,Y., Li,B., Liu,Z., Li,W., Li,K., Tan,S.,
            Zhu,Q., Tang,Z., Han,F., Wu,Z., Shen,L., Jiang,H., Tang,B., Qiu,J.,
            Hu,Z. and Wang,J.
  TITLE     Association of TRMT2B gene variants with juvenile amyotrophic
            lateral sclerosis
  JOURNAL   Front Med 18 (1), 68-80 (2024)
   PUBMED   37874476
  REMARK    GeneRIF: Association of TRMT2B gene variants with juvenile
            amyotrophic lateral sclerosis.
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 3547)
  AUTHORS   Dai,W., Li,A., Yu,N.J., Nguyen,T., Leach,R.W., Wuhr,M. and
            Kleiner,R.E.
  TITLE     Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated
            dihydrouridylation
  JOURNAL   Nat Chem Biol 17 (11), 1178-1187 (2021)
   PUBMED   34556860
REFERENCE   4  (bases 1 to 3547)
  AUTHORS   Powell,C.A. and Minczuk,M.
  TITLE     TRMT2B is responsible for both tRNA and rRNA m5U-methylation in
            human mitochondria
  JOURNAL   RNA Biol 17 (4), 451-462 (2020)
   PUBMED   31948311
  REMARK    GeneRIF: TRMT2B is responsible for both tRNA and rRNA
            m(5)U-methylation in human mitochondria.
REFERENCE   5  (bases 1 to 3547)
  AUTHORS   Lee,S.Y., Kang,M.G., Park,J.S., Lee,G., Ting,A.Y. and Rhee,H.W.
  TITLE     APEX Fingerprinting Reveals the Subcellular Localization of
            Proteins of Interest
  JOURNAL   Cell Rep 15 (8), 1837-1847 (2016)
   PUBMED   27184847
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA454040.1, BC034272.2 and
            Z97985.16.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_024917.5.
            
            Summary: This gene encodes a homolog of the TRM2 gene in S.
            cerevisiae. The yeast gene encodes a tRNA methyltransferase that
            plays a role in tRNA maturation. The yeast protein also has
            endo-exonuclease activity and may be involved in DNA double strand
            break repair. Alternative splicing results in multiple transcripts
            encoding different isoforms. [provided by RefSeq, Nov 2009].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1, 2, and 4 all encode isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC034272.2, SRR14038197.200797.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372936.4/ ENSP00000362027.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                DA454040.1         6-35
            31-2329             BC034272.2         1-2299
            2330-3547           Z97985.16          68814-70031         c
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq22.1"
     gene            1..3547
                     /gene="TRMT2B"
                     /gene_synonym="CXorf34; dJ341D10.3"
                     /note="tRNA methyltransferase 2 homolog B"
                     /db_xref="GeneID:79979"
                     /db_xref="HGNC:HGNC:25748"
                     /db_xref="MIM:301128"
     exon            1..295
                     /gene="TRMT2B"
                     /gene_synonym="CXorf34; dJ341D10.3"
                     /inference="alignment:Splign:2.1.0"
     exon            296..778
                     /gene="TRMT2B"
                     /gene_synonym="CXorf34; dJ341D10.3"
                     /inference="alignment:Splign:2.1.0"
     exon            779..1049
                     /gene="TRMT2B"
                     /gene_synonym="CXorf34; dJ341D10.3"
                     /inference="alignment:Splign:2.1.0"
     CDS             802..2316
                     /gene="TRMT2B"
                     /gene_synonym="CXorf34; dJ341D10.3"
                     /EC_number="2.1.1.35"
                     /note="isoform a is encoded by transcript variant 1; tRNA
                     (uracil-5-)-methyltransferase homolog; TRM2 homolog; tRNA
                     (uracil(54)-C(5))-methyltransferase homolog; TRM2 tRNA
                     methyltransferase 2 homolog B; TRM2 homolog B; rRNA
                     (uracil-5-)-methyltransferase TRMT2B; tRNA
                     (uracil-5-)-methyltransferase homolog B"
                     /codon_start=1
                     /product="tRNA (uracil-5-)-methyltransferase homolog B
                     isoform a"
                     /protein_id="NP_079193.2"
                     /db_xref="CCDS:CCDS14477.1"
                     /db_xref="GeneID:79979"
                     /db_xref="HGNC:HGNC:25748"
                     /db_xref="MIM:301128"
                     /translation="MAGLKRRVPLHSLRYFISMVGLFSKPGLLPWYARNPPGWSQLFL
                     GTVCKGDFTRVIATKCQKGQKSQKKPSHLGPLDGSWQERLADVVTPLWRLSYEEQLKV
                     KFAAQKKILQRLESYIQMLNGVSVTTAVPKSERLSCLLHPIIPSPVINGYRNKSTFSV
                     NRGPDGNPKTVGFYLGTWRDGNVVCVQSNHLKNIPEKHSQVAQYYEVFLRQSPLEPCL
                     VFHEGGYWRELTVRTNSQGHTMAIITFHPQKLSQEELHVQKEIVKEFFIRGPGAACGL
                     TSLYFQESTMTRCSHQQSPYQLLFGEPYIFEELLSLKIRISPDAFFQINTAGAEMLYR
                     TVGELTGVNSDTILLDICCGTGVIGLSLAQHTSRVLGIELLEQAVEDARWTAAFNGIT
                     NSEFHTGQAEKILPGLLKSKEDGQSIVAVVNPARAGLHYKVIQAIRNFRAIHTLVFVS
                     CKLHGESTRNVIELCCPPDPAKKLLGEPFVLQQAVPVDLFPHTPHCELVLLFTR"
     exon            1050..1104
                     /gene="TRMT2B"
                     /gene_synonym="CXorf34; dJ341D10.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1239
                     /gene="TRMT2B"
                     /gene_synonym="CXorf34; dJ341D10.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1339
                     /gene="TRMT2B"
                     /gene_synonym="CXorf34; dJ341D10.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1410
                     /gene="TRMT2B"
                     /gene_synonym="CXorf34; dJ341D10.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1557
                     /gene="TRMT2B"
                     /gene_synonym="CXorf34; dJ341D10.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1558..1652
                     /gene="TRMT2B"
                     /gene_synonym="CXorf34; dJ341D10.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1653..1867
                     /gene="TRMT2B"
                     /gene_synonym="CXorf34; dJ341D10.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1868..1969
                     /gene="TRMT2B"
                     /gene_synonym="CXorf34; dJ341D10.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1970..2089
                     /gene="TRMT2B"
                     /gene_synonym="CXorf34; dJ341D10.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2090..2189
                     /gene="TRMT2B"
                     /gene_synonym="CXorf34; dJ341D10.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2190..3547
                     /gene="TRMT2B"
                     /gene_synonym="CXorf34; dJ341D10.3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3524..3529
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRMT2B"
                     /gene_synonym="CXorf34; dJ341D10.3"
                     /note="hexamer: AATAAA"
     polyA_site      3547
                     /gene="TRMT2B"
                     /gene_synonym="CXorf34; dJ341D10.3"
                     /note="major polyA site"
ORIGIN      
        1 aggagaggag aacttagaga aggaaggcgg cgggtgtcag ggctatgagt gcttttaagg
       61 aaggtctcca tagaaggttc ccccagcaaa gtagggaggg gtactccccg agaggagtcg
      121 aggtgccgga gcctgctgcc tcgccgacgc tgggaggagc cccgtgtccc gccgcgcttg
      181 ccaagggttg gctgcgcgtg cggcgggagt agaggcgcct tgcgcaccag gaagtgactg
      241 tttccccgcc gcagcaaacc aggccatccg ctggcctttt agtttgccgc ctcagaaaac
      301 tccctactat gccaaactgt ccgcggggcg gggaatggag gaggcaggcg gggctggaga
      361 cgggacccga cggtgactgc gcaaggtcgt gtacggccca gcgtgggcct gtgccctaac
      421 tcctcccact cccttacttt ctgtccttcc cgggccctgc agaccggcca agtttagtgg
      481 tagcctttcc tagtgtctcc tctttgttaa cccttcctac agacacagtg ggcagaactt
      541 gcctaaagaa gaagggaatg gaagggctag tggagtcttg ttggggagac aaggacctga
      601 ctgcctgtgg gctgcattcg ggacacgaca cagcagtcaa cccatttgct tgtttggttt
      661 agcaaggaat agtgtctgga gccggtccag ctgaaggaag ggtgagcaga gagtgcaagc
      721 aggacccttg tcggtgggtg aacattttgc tccaaaaggg atccttggcc ctgcgcaggt
      781 ggatttcagt gtgttctttg gatggcaggc cttaagagaa gagtcccact gcacagcctc
      841 agatacttca tctccatggt gggtctcttc tccaaaccag gactgcttcc ctggtatgcc
      901 agaaatccac caggatggtc acagctcttt ctgggcacag tatgtaaggg agatttcacc
      961 cgtgtgatag ccacgaaatg tcagaaagga caaaaaagtc agaagaaacc aagccatctt
     1021 ggaccactag atggttcctg gcaggaaagg ctggctgatg ttgtgacacc actctggagg
     1081 ttgagctatg aagaacagct caaggtgaaa tttgcagctc agaagaaaat tttacaaaga
     1141 ctagagtctt acatccaaat gctcaatgga gtcagtgtga caacggctgt acccaaatct
     1201 gagaggctct cttgtcttct ccatcctatt ataccctctc ctgtcatcaa tggttaccga
     1261 aataagtcca ccttctctgt gaaccgaggt ccagatggca atccaaagac tgtggggttc
     1321 tacctgggaa cttggagaga tgggaacgtt gtctgtgtgc agtctaatca tctgaaaaac
     1381 atccctgaga aacacagtca agtggcgcag tactatgaag tattccttcg acagtctcca
     1441 ttggagccct gccttgtatt tcatgaaggt ggatactggc gtgagctcac agtccgcacc
     1501 aatagccaag ggcacacaat ggctatcatc actttccatc cccagaaatt aagtcaggag
     1561 gagctccatg ttcagaagga gattgtaaag gaatttttca tcagaggtcc tggagcagcc
     1621 tgtggcttga cctcacttta cttccaggaa agtaccatga cccgttgcag ccatcagcag
     1681 tctccctatc agcttctgtt tggggaaccc tacatctttg aagaacttct gagcttgaag
     1741 atccgcatct ctccagatgc ctttttccag attaacacag ctggtgcaga gatgctgtat
     1801 cggactgtgg gggagctgac tggagtgaac tctgacacca tccttcttga catctgctgt
     1861 ggaactggtg tgattggcct ctctctggct cagcatacat ctcgggtcct tgggattgaa
     1921 ttgttggagc aggcagtgga ggatgcaaga tggactgcag ccttcaatgg catcaccaac
     1981 tctgaatttc atactggtca agcagagaag attttgccag ggctgctaaa gtcaaaggaa
     2041 gatggacagt caattgttgc tgtggtgaac ccagcccgtg ccggactgca ttacaaggtg
     2101 attcaagcca ttcgaaactt cagggccatc cacacgctag tttttgtttc ctgcaagctc
     2161 catggtgaat ccactaggaa tgtcattgag ctgtgctgtc ctccagaccc tgctaagaag
     2221 ctcttaggcg agccctttgt cctgcagcaa gctgtacctg tggatttgtt ccctcacacc
     2281 ccacattgtg agctggtgct cctctttact cgataagcag cctcctagaa gacagcaggc
     2341 tatttgttaa ggctgaagtt tcagaaactt tcaggtttgg catattgcta cattgcagac
     2401 cctgggagca ttacagtgga aaccaatctt tggtttgtga ataggaagaa tctattaggc
     2461 ctctggtctg aggcagattt tttttttgag atagagtctc actcttgccc aggctggagt
     2521 gtcgtggcgt gatctcagct cactgcaacc tccgcctccc gggttcaagt gattctcgtg
     2581 cctcagcttc cccagtagct aggattaaag gtgcgtgcca ccatgcccgg ctaatttttg
     2641 tatttttggt agagacaggg tttcaccatg ttggccaggc tggtctcaaa ctcctgacct
     2701 caagtgatcc acccgcctcg gcctctcaaa gtgctgggat tataggcgtg agccaccaca
     2761 ccccgccgcg gctgacttaa ccttagattt cagattccct ttcaatcatg cccgtttgct
     2821 tgggtttgac cactccccac tcctggatct ggactgtttc ttcctgttgc tgtcagcctt
     2881 ggttctagtt tatagtcttg tctcctccgt ttctgcttat ggtgtggaca aatgaccttt
     2941 tttaagcagc tggtaataat tttacattga ctggagcctg tgcatttgct cagagatgct
     3001 acaattttta gatatttcta agacttctgg attcttgagg tagttaagga ttaaaaccag
     3061 aaacagtcga ggttttttaa aagcagggta agccatgaag tgaatacagg gtgactgagt
     3121 gatcatcaga tgagattaat tcttttgggt tttcttggtt ttaaggcatc tcgaatgccc
     3181 cttgaaaatg tagttttccc tggtggtggg cacctgtaat cccagctact cgggaggctg
     3241 aggcaggata attgcttgaa cctgggaggt ggaggttgca gtgagctgaa attgtgccac
     3301 tgcactccag cctgggtgac agagtgagac accatctcaa aaaaaaaaaa aaaaagaaaa
     3361 tgtagttatc cctaggagca atagcaccaa ttaactctca cccgtggagg aagaattggt
     3421 tattggagag accctaaggg aaaggagatt ttcagagaga ctgaatacct ataattgtgc
     3481 tgtgtagtgt ggaggcaaaa agctccaaaa tctagtataa cataataaac tggatattga
     3541 aaactga
//
