LOCUS       NM_017956               2207 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens tRNA methyltransferase 12 homolog (TRMT12), mRNA.
ACCESSION   NM_017956
VERSION     NM_017956.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Rossello-Tortella,M., Llinas-Arias,P., Sakaguchi,Y., Miyauchi,K.,
            Davalos,V., Setien,F., Calleja-Cervantes,M.E., Pineyro,D.,
            Martinez-Gomez,J., Guil,S., Joshi,R., Villanueva,A., Suzuki,T. and
            Esteller,M.
  TITLE     Epigenetic loss of the transfer RNA-modifying enzyme TYW2 induces
            ribosome frameshifts in colon cancer
  JOURNAL   Proc Natl Acad Sci U S A 117 (34), 20785-20793 (2020)
   PUBMED   32778592
  REMARK    GeneRIF: Epigenetic loss of the transfer RNA-modifying enzyme TYW2
            induces ribosome frameshifts in colon cancer.
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 2207)
  AUTHORS   Rodriguez,V., Vasudevan,S., Noma,A., Carlson,B.A., Green,J.E.,
            Suzuki,T. and Chandrasekharappa,S.C.
  TITLE     Structure-function analysis of human TYW2 enzyme required for the
            biosynthesis of a highly modified Wybutosine (yW) base in
            phenylalanine-tRNA
  JOURNAL   PLoS One 7 (6), e39297 (2012)
   PUBMED   22761755
REFERENCE   4  (bases 1 to 2207)
  AUTHORS   Rodriguez,V., Chen,Y., Elkahloun,A., Dutra,A., Pak,E. and
            Chandrasekharappa,S.
  TITLE     Chromosome 8 BAC array comparative genomic hybridization and
            expression analysis identify amplification and overexpression of
            TRMT12 in breast cancer
  JOURNAL   Genes Chromosomes Cancer 46 (7), 694-707 (2007)
   PUBMED   17440925
  REMARK    GeneRIF: analysis by RT-qPCR using RNA from 30 breast tumors showed
            TRMT12 was overexpressed >2 fold in 87% of the tumors. TRMT12 is a
            homologue of a yeast gene encoding a tRNA methyltransferase
            involved in the posttranscriptional modification of tRNA(Phe)
REFERENCE   5  (bases 1 to 2207)
  AUTHORS   Noma,A. and Suzuki,T.
  TITLE     Ribonucleome analysis identified enzyme genes responsible for
            wybutosine synthesis
  JOURNAL   Nucleic Acids Symp Ser (Oxf) (50), 65-66 (2006)
   PUBMED   17150819
REFERENCE   6  (bases 1 to 2207)
  AUTHORS   Kalhor,H.R., Penjwini,M. and Clarke,S.
  TITLE     A novel methyltransferase required for the formation of the
            hypermodified nucleoside wybutosine in eucaryotic tRNA
  JOURNAL   Biochem Biophys Res Commun 334 (2), 433-440 (2005)
   PUBMED   16005430
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP342608.1, AK222727.1 and AA608716.1.
            
            On May 17, 2019 this sequence version replaced NM_017956.3.
            
            Summary: Wybutosine (yW) is a hypermodified guanosine at the
            3-prime position adjacent to the anticodon of phenylalanine tRNA
            that stabilizes codon-anticodon interactions during decoding on the
            ribosome. TRMT12 is the human homolog of a yeast gene essential for
            yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM, Mar 2008].
            
            ##Evidence-Data-START##
            Transcript is intronless :: AK225083.1, SRR1660803.138373.1
                                        [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000328599.4/ ENSP00000329858.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                BP342608.1         14-50
            38-2016             AK222727.1         39-2017
            2017-2207           AA608716.1         13-203              c
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.13"
     gene            1..2207
                     /gene="TRMT12"
                     /gene_synonym="TRM12; TYW2"
                     /note="tRNA methyltransferase 12 homolog"
                     /db_xref="GeneID:55039"
                     /db_xref="HGNC:HGNC:26091"
                     /db_xref="MIM:611244"
     exon            1..2207
                     /gene="TRMT12"
                     /gene_synonym="TRM12; TYW2"
                     /inference="alignment:Splign:2.1.0"
     CDS             109..1455
                     /gene="TRMT12"
                     /gene_synonym="TRM12; TYW2"
                     /EC_number="2.5.1.114"
                     /note="homolog of yeast tRNA methyltransferase;
                     tRNA-yW-synthesizing protein 2;
                     alpha-amino-alpha-carboxypropyl transferase TYW2; tRNA
                     methyltranferase 12 homolog; tRNA(Phe)
                     (4-demethylwyosine(37)-C(7))
                     aminocarboxypropyltransferase"
                     /codon_start=1
                     /product="tRNA wybutosine-synthesizing protein 2 homolog"
                     /protein_id="NP_060426.2"
                     /db_xref="CCDS:CCDS6349.1"
                     /db_xref="GeneID:55039"
                     /db_xref="HGNC:HGNC:26091"
                     /db_xref="MIM:611244"
                     /translation="MRENVVVSNMERESGKPVAVVAVVTEPWFTQRYREYLQRQKLFD
                     TQHRVEKMPDGSVALPVLGETLPEQHLQELRNRVAPGSPCMLTQLPDPVPSKRAQGCS
                     PAQKLCLEVSRWVEGRGVKWSAELEADLPRSWQRHGNLLLLSEDCFQAKQWKNLGPEL
                     WETVALALGVQRLAKRGRVSPDGTRTPAVTLLLGDHGWVEHVDNGIRYKFDVTQCMFS
                     FGNITEKLRVASLSCAGEVLVDLYAGIGYFTLPFLVHAGAAFVHACEWNPHAVVALRN
                     NLEINGVADRCQIHFGDNRKLKLSNIADRVILGLIPSSEEGWPIACQVLRQDAGGILH
                     IHQNVESFPGKNLQALGVSKVEKEHWLYPQQITTNQWKNGATRDSRGKMLSPATKPEW
                     QRWAESAETRIATLLQQVHGKPWKTQILHIQPVKSYAPHVDHIVLDLECCPCPSVG"
     regulatory      2017..2022
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRMT12"
                     /gene_synonym="TRM12; TYW2"
                     /note="hexamer: AATAAA"
     polyA_site      2035
                     /gene="TRMT12"
                     /gene_synonym="TRM12; TYW2"
                     /note="major polyA site"
     regulatory      2181..2186
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRMT12"
                     /gene_synonym="TRM12; TYW2"
                     /note="hexamer: TATAAA"
     polyA_site      2207
                     /gene="TRMT12"
                     /gene_synonym="TRM12; TYW2"
ORIGIN      
        1 atttccggca ccggcatggc cgggtgagct gcaggctacc ttatttaaga ccgggaattt
       61 agtcagcctg gtgagccgac tctgaggaga tggagtatcg ctgaggtgat gagagagaat
      121 gtggttgtta gcaacatgga gagagaaagt gggaagcccg tggctgttgt cgcagttgtg
      181 actgagcctt ggtttaccca gcgatacaga gaatatctcc agaggcagaa actctttgat
      241 acacagcacc gtgtggaaaa gatgccggat ggctcggtgg cgctaccggt gctgggagag
      301 acgcttccag agcagcacct gcaggagctg aggaatcgtg ttgccccagg cagtccctgt
      361 atgctcacgc agctcccgga tcctgttcct tcgaagaggg cccagggttg ttcacctgcc
      421 caaaaattgt gtcttgaggt gagtcgctgg gtggagggtc ggggagtcaa gtggtcagcc
      481 gagttggagg ctgatttgcc ccgatcatgg caacggcatg gtaatctctt gttgctgagt
      541 gaagactgtt tccaagccaa gcagtggaaa aatctgggac cggaactctg ggagaccgtt
      601 gccttggcac ttggcgtcca gcgtttggca aaacgagggc gggtatcacc ggatggtact
      661 cgaactccag cagtgacact gctgctgggt gaccatggct gggtagagca tgtggataat
      721 ggtatccgtt ataagtttga cgtgacccag tgtatgttct cctttggaaa catcactgag
      781 aagcttcgag tggcatcgtt gtcctgtgct ggagaagtgc tggtggatct ctatgcaggg
      841 attggttatt ttacattgcc tttcctagtt catgctggtg ctgccttcgt ccatgcttgt
      901 gagtggaatc cccatgctgt agttgctctg agaaataacc ttgagatcaa tggagtagca
      961 gatcggtgcc aaatacactt tggagataac agaaaactga agctctcaaa tattgcagat
     1021 agggtgatcc tggggctgat tcccagctct gaagaaggct ggcccattgc ctgccaagtg
     1081 ttaaggcagg atgctggagg cattttgcat atccaccaaa atgtggaatc tttcccaggg
     1141 aagaatcttc aggctcttgg agtcagcaaa gtagagaaag agcattggct gtatcctcag
     1201 caaattacca ccaaccaatg gaaaaatgga gctaccaggg attctagggg aaaaatgctg
     1261 tcaccagcca ccaagccaga gtggcaaagg tgggcagaat ctgcagaaac tcgaatcgcc
     1321 actcttcttc agcaggtgca tgggaaacca tggaagacac aaattctgca catccaacca
     1381 gtgaaatcct atgctcccca tgtggatcac atagtcctgg atctggaatg ctgcccctgt
     1441 ccttcagttg gctagaggag gtagatcctg ggacacatgg gatccacgtg cgagtggccc
     1501 ttaaatgtat cagttcagtc caggttgtca tcccttttgt cccctggtga tcagtttttt
     1561 tcatatttta tagccctgaa agcaggctct agatcaattc aaattatttc atttgtcttt
     1621 cattgataac agaaaatgaa atacctgttt gggagaagca gcatggccca ttgaaatgag
     1681 gctcatctgt gcaattatga attccaaatt ctgacctcag ttctggaatt gaagtttcag
     1741 tatgttttgg cctcgggttt cgttatttgc aaaatgagag tttctttgaa ctgtctcacg
     1801 tgactattaa gcaactatac acaggacatc ggttatttta gagtgaaaga cacagtgctt
     1861 tttccaaatt gctctggcta ccatatagaa aattgactga aggagggcca agatggaaac
     1921 agagagacca gtgaggaggc ttctgtggtt gtccaggtct gaggtgatgg taacttggac
     1981 tcggatggtg gtaatgggag gtagattgat atgataaata aaattgacag accaagcaat
     2041 ggaatcagaa ttaagtcctt aacatgaagc tgctttgtta ttatgactga ctaaattaga
     2101 gagagaaggg aacaaaaatt attttagtgt atttccattc ccttactgtg gcattcctaa
     2161 atgattgtga ggggttgtct tataaatttg gtttgttgtg gtagaaa
//
