LOCUS       NM_144964               2293 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens tRNA methyltransferase 10B (TRMT10B), transcript
            variant 1, mRNA.
ACCESSION   NM_144964 NM_001077260
VERSION     NM_144964.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Qi,L., Wang,Q., Guan,Z., Wu,Y., Shen,C., Hong,S., Cao,J., Zhang,X.,
            Yan,C. and Yin,P.
  TITLE     Cryo-EM structure of the human mitochondrial translocase TIM22
            complex
  JOURNAL   Cell Res 31 (3), 369-372 (2021)
   PUBMED   32901109
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Vilardo,E., Amman,F., Toth,U., Kotter,A., Helm,M. and Rossmanith,W.
  TITLE     Functional characterization of the human tRNA methyltransferases
            TRMT10A and TRMT10B
  JOURNAL   Nucleic Acids Res 48 (11), 6157-6169 (2020)
   PUBMED   32392304
  REMARK    GeneRIF: Functional characterization of the human tRNA
            methyltransferases TRMT10A and TRMT10B.
REFERENCE   3  (bases 1 to 2293)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2293)
  AUTHORS   Howell,N.W., Jora,M., Jepson,B.F., Limbach,P.A. and Jackman,J.E.
  TITLE     Distinct substrate specificities of the human tRNA
            methyltransferases TRMT10A and TRMT10B
  JOURNAL   RNA 25 (10), 1366-1376 (2019)
   PUBMED   31292261
  REMARK    GeneRIF: demonstrate that human TRMT10A (hTRMT10A) and human
            TRMT10B (hTRMT10B) are not biochemically redundant
REFERENCE   5  (bases 1 to 2293)
  AUTHORS   Vilardo,E., Nachbagauer,C., Buzet,A., Taschner,A., Holzmann,J. and
            Rossmanith,W.
  TITLE     A subcomplex of human mitochondrial RNase P is a bifunctional
            methyltransferase--extensive moonlighting in mitochondrial tRNA
            biogenesis
  JOURNAL   Nucleic Acids Res 40 (22), 11583-11593 (2012)
   PUBMED   23042678
  REMARK    Erratum:[Nucleic Acids Res. 2018 Nov 16;46(20):11126-11127. doi:
            10.1093/nar/gky931.. PMID: 30295808]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            HY066525.1, BC057774.1, AK056017.1, AK092718.1 and CA440955.1.
            
            On Nov 22, 2018 this sequence version replaced NM_144964.3.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK056017.1, SRR1803613.97173.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000297994.4/ ENSP00000297994.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                HY066525.1         3-13
            12-1359             BC057774.1         1-1348
            1360-1766           AK056017.1         1360-1766
            1767-1821           AK092718.1         1650-1704
            1822-2293           CA440955.1         16-487              c
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p13.2"
     gene            1..2293
                     /gene="TRMT10B"
                     /gene_synonym="bA3J10.9; RG9MTD3"
                     /note="tRNA methyltransferase 10B"
                     /db_xref="GeneID:158234"
                     /db_xref="HGNC:HGNC:26454"
                     /db_xref="MIM:620957"
     exon            1..48
                     /gene="TRMT10B"
                     /gene_synonym="bA3J10.9; RG9MTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            49..263
                     /gene="TRMT10B"
                     /gene_synonym="bA3J10.9; RG9MTD3"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..1028
                     /gene="TRMT10B"
                     /gene_synonym="bA3J10.9; RG9MTD3"
                     /EC_number="2.1.1.221"
                     /note="isoform a is encoded by transcript variant 1; RNA
                     (guanine-9-)-methyltransferase domain-containing protein
                     3; RNA (guanine-9-) methyltransferase domain containing 3;
                     tRNA methyltransferase 10 homolog B; tRNA
                     (guanine(9)-N(1))-methyltransferase TRMT10B"
                     /codon_start=1
                     /product="tRNA methyltransferase 10 homolog B isoform a"
                     /protein_id="NP_659401.2"
                     /db_xref="CCDS:CCDS43804.1"
                     /db_xref="GeneID:158234"
                     /db_xref="HGNC:HGNC:26454"
                     /db_xref="MIM:620957"
                     /translation="MDWKLEGSTQKVESPVLQGQEGILEETGEDGLPEGFQLLQIDAE
                     GECQEGEILATGSTAWCSKNVQRKQRHWEKIVAAKKSKRKQEKERRKANRAENPGICP
                     QHSKRFLRALTKDKLLEAKHSGPRLCIDLSMTHYMSKKELSRLAGQIRRLYGSNKKAD
                     RPFWICLTGFTTDSPLYEECVRMNDGFSSYLLDITEEDCFSLFPLETLVYLTPDSEHA
                     LEDVDLNKVYILGGLVDESIQKKVTFQKAREYSVKTARLPIQEYMVRNQNGKNYHSEI
                     LAINQVFDILSTYLETHNWPEALKKGVSSGKGYILRNSVE"
     exon            264..372
                     /gene="TRMT10B"
                     /gene_synonym="bA3J10.9; RG9MTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            373..497
                     /gene="TRMT10B"
                     /gene_synonym="bA3J10.9; RG9MTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            498..650
                     /gene="TRMT10B"
                     /gene_synonym="bA3J10.9; RG9MTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            651..729
                     /gene="TRMT10B"
                     /gene_synonym="bA3J10.9; RG9MTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            730..797
                     /gene="TRMT10B"
                     /gene_synonym="bA3J10.9; RG9MTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            798..921
                     /gene="TRMT10B"
                     /gene_synonym="bA3J10.9; RG9MTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            922..2293
                     /gene="TRMT10B"
                     /gene_synonym="bA3J10.9; RG9MTD3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1980..1985
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRMT10B"
                     /gene_synonym="bA3J10.9; RG9MTD3"
                     /note="hexamer: CATAAA"
     polyA_site      2003
                     /gene="TRMT10B"
                     /gene_synonym="bA3J10.9; RG9MTD3"
                     /note="major polyA site"
     regulatory      2272..2277
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRMT10B"
                     /gene_synonym="bA3J10.9; RG9MTD3"
                     /note="hexamer: ATTAAA"
     polyA_site      2293
                     /gene="TRMT10B"
                     /gene_synonym="bA3J10.9; RG9MTD3"
                     /note="major polyA site"
ORIGIN      
        1 ggggatccga tgcgcgccgc tgccgctgcg tgggggtgag gggatcagtc tggtggaaag
       61 gacagaggac taagtccatg gactggaaat tggaagggag tactcagaaa gtagagtcac
      121 ctgtgctgca ggggcaagaa ggcatcctag aggagacagg tgaagatgga cttcctgaag
      181 gcttccagct tctgcagatc gatgcggaag gcgagtgcca ggagggagag atcctggcca
      241 caggcagtac ggcatggtgc tcgaaaaatg tccagagaaa acagagacac tgggaaaaga
      301 tagttgcagc aaagaagagc aaaagaaagc aagaaaaaga acgaagaaaa gccaatcgtg
      361 cagaaaatcc aggcatttgc ccccagcaca gcaaacgttt cctgagagct ctaaccaaag
      421 acaaactttt ggaagccaaa cactcaggac caagactatg tatcgatttg agtatgaccc
      481 actacatgtc aaagaaggaa ttaagtagac tggctggaca gattcgaagg ttgtatggtt
      541 caaacaaaaa agctgacagg ccattttgga tctgcctcac tggattcaca acagacagtc
      601 ccctttatga agagtgtgtg aggatgaatg atggattttc tagttacctg ttagacataa
      661 cagaagaaga ctgctttagt ttatttccct tggaaaccct tgtgtacctg actcctgact
      721 cagaacacgc tcttgaagat gttgatctaa acaaagttta catcctcggt gggcttgtgg
      781 atgaaagcat tcagaagaag gtgacatttc aaaaggcccg ggaatactct gtcaagaccg
      841 cacgcttgcc aatccaggaa tacatggtca gaaaccagaa tgggaaaaac tatcattcag
      901 agatactggc catcaatcaa gtgtttgata tcctgtccac ttacttagag actcacaact
      961 ggcctgaagc attgaagaaa ggagtttctt caggaaaagg ctatattctt cggaactcag
     1021 tggaatgatg ggcctaagat tgcagctgcg tggccaggtg ctcacgccgt aatgccaaca
     1081 ctttggtaga ccgaagtggg cagatcacct gaggtcagga gttcacgacc agcctggcca
     1141 acatggtgaa acccttctct actgaaaata caaaaattag ccaggtgtgg tggcgcatac
     1201 ctgtagtccc agctacttgg gaggctgagg caggagaatc acttgaactc gggaggcgaa
     1261 ggttgcagtg agccgagatt tcaccagtgc actccagcct gggtgacaga gcaagactcc
     1321 atctcaaaaa aaaaataaat aaaaaaaaat gcagctgcag gagtgaggcg cttggaggta
     1381 ccttgaccca aagagcaggg cagagggtgg cagtggcaca taggcaagtg tctttgcatg
     1441 acatcttctc agagcttcac aataatgtca gggaccacat ttaatgcttt ttaatctccc
     1501 atagtgcctg gctcacagga agtgctcaga tatgttaagt aataaaaagt taatgtggtg
     1561 ggtgcagtgg ctcacgcctg taatcccagc actttgggag gctgaggtga gtggattaca
     1621 aggtcaggag ttccagacca gcctggctaa tatggtgaaa cctcgtctct actaaaaata
     1681 caaaaattag ctgggcatgg tggtgcgtgc ctgtagtctc agctactcag gaggctgagg
     1741 caggagaatc gcctgaaccc gggcggcgga ggttgtagtg agccaagatc cagcctgggc
     1801 gacagagcca gactcttgtc tcgaggggaa aaaaaaaaaa aattatgtaa caaggaatta
     1861 gcctcaaaac cacatggctt tggacgtgct aattcaactt ttgtacatct atttgtgaga
     1921 gacctcttga aattgtttaa atggttacat caagagttta atattcacta aactggcatc
     1981 ataaaattgt atgactttct ccagccttgt ctgatcatgc tcgttgttgc ccagctcagt
     2041 aataagcttt gcaggatcca gcctgttctg acctatccct actgagtccc catagcattt
     2101 tgttccctcc tgtggctctt accttgttct cccttgcaga gtatgggcat ggagggcttc
     2161 tcttcctaat ccttcctaat ggtaggatcc taacaggaca aatctgtgta cctatctttc
     2221 tatcctccct gagcctgtac caactagatt cttcatatgt atttgtaaca gattaaattg
     2281 gaaagcaaac aca
//
