LOCUS       NM_001039141           10085 bp    mRNA    linear   PRI 11-DEC-2024
DEFINITION  Homo sapiens TRIO and F-actin binding protein (TRIOBP), transcript
            variant 6, mRNA.
ACCESSION   NM_001039141
VERSION     NM_001039141.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Wang,L., Zhao,W., Xia,C., Ma,S., Li,Z., Wang,N., Ding,L., Wang,Y.,
            Cheng,L., Liu,H., Yang,J., Li,Y., Rosas,I. and Yu,G.
  TITLE     TRIOBP modulates beta-catenin signaling by regulation of miR-29b in
            idiopathic pulmonary fibrosis
  JOURNAL   Cell Mol Life Sci 81 (1), 13 (2023)
   PUBMED   38157020
  REMARK    GeneRIF: TRIOBP modulates beta-catenin signaling by regulation of
            miR-29b in idiopathic pulmonary fibrosis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10085)
  AUTHORS   Pavesic Radonja,A., Blazevic Zelic,S., Rubesa,G. and Bradshaw,N.J.
  TITLE     SYMPTOM SEVERITY IN SCHIZOPHRENIA PATIENTS WITH NPAS3, DYSBINDIN-1
            AND/OR TRIOBP PROTEIN PATHOLOGY IN THEIR BLOOD SERUM: A PANSS-BASED
            FOLLOW UP STUDY
  JOURNAL   Psychiatr Danub 35 (2), 180-186 (2023)
   PUBMED   37480305
  REMARK    GeneRIF: SYMPTOM SEVERITY IN SCHIZOPHRENIA PATIENTS WITH NPAS3,
            DYSBINDIN-1 AND/OR TRIOBP PROTEIN PATHOLOGY IN THEIR BLOOD SERUM: A
            PANSS-BASED FOLLOW UP STUDY.
REFERENCE   3  (bases 1 to 10085)
  AUTHORS   Kabahuma,R.I., Schubert,W.D., Labuschagne,C., Yan,D., Pepper,M.S.
            and Liu,X.Z.
  TITLE     Elucidation of repeat motifs R1- and R2-related TRIOBP variants in
            autosomal recessive nonsyndromic hearing loss DFNB28 among
            indigenous South African individuals
  JOURNAL   Mol Genet Genomic Med 10 (10), e2015 (2022)
   PUBMED   36029164
  REMARK    GeneRIF: Elucidation of repeat motifs R1- and R2-related TRIOBP
            variants in autosomal recessive nonsyndromic hearing loss DFNB28
            among indigenous South African individuals.
REFERENCE   4  (bases 1 to 10085)
  AUTHORS   Zaharija,B., Odorcic,M., Hart,A., Samardzija,B., Marreiros,R.,
            Prikulis,I., Jukovic,M., Hyde,T.M., Kleinman,J.E., Korth,C. and
            Bradshaw,N.J.
  TITLE     TRIOBP-1 Protein Aggregation Exists in Both Major Depressive
            Disorder and Schizophrenia, and Can Occur through Two Distinct
            Regions of the Protein
  JOURNAL   Int J Mol Sci 23 (19), 11048 (2022)
   PUBMED   36232351
  REMARK    GeneRIF: TRIOBP-1 Protein Aggregation Exists in Both Major
            Depressive Disorder and Schizophrenia, and Can Occur through Two
            Distinct Regions of the Protein.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10085)
  AUTHORS   Park,S., Lee,H., Kim,M., Park,J., Kim,S.H. and Park,J.
  TITLE     Emerging roles of TRIO and F-actin-binding protein in human
            diseases
  JOURNAL   Cell Commun Signal 16 (1), 29 (2018)
   PUBMED   29890989
  REMARK    GeneRIF: roles of TRIO and F-actin-binding protein in human
            diseases
            Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 10085)
  AUTHORS   Seipel,K., O'Brien,S.P., Iannotti,E., Medley,Q.G. and Streuli,M.
  TITLE     Tara, a novel F-actin binding protein, associates with the Trio
            guanine nucleotide exchange factor and regulates actin cytoskeletal
            organization
  JOURNAL   J Cell Sci 114 (Pt 2), 389-399 (2001)
   PUBMED   11148140
REFERENCE   7  (bases 1 to 10085)
  AUTHORS   Dunham,I., Shimizu,N., Roe,B.A., Chissoe,S., Hunt,A.R.,
            Collins,J.E., Bruskiewich,R., Beare,D.M., Clamp,M., Smink,L.J.,
            Ainscough,R., Almeida,J.P., Babbage,A., Bagguley,C., Bailey,J.,
            Barlow,K., Bates,K.N., Beasley,O., Bird,C.P., Blakey,S.,
            Bridgeman,A.M., Buck,D., Burgess,J., Burrill,W.D., O'Brien,K.P. et
            al.
  TITLE     The DNA sequence of human chromosome 22
  JOURNAL   Nature 402 (6761), 489-495 (1999)
   PUBMED   10591208
  REMARK    Erratum:[Nature 2000 Apr 20;404(6780):904]
REFERENCE   8  (bases 1 to 10085)
  AUTHORS   Ueki,N., Oda,T., Kondo,M., Yano,K., Noguchi,T. and Muramatsu,M.
  TITLE     Selection system for genes encoding nuclear-targeted proteins
  JOURNAL   Nat Biotechnol 16 (13), 1338-1342 (1998)
   PUBMED   9853615
REFERENCE   9  (bases 1 to 10085)
  AUTHORS   Mayer,B.J., Ren,R., Clark,K.L. and Baltimore,D.
  TITLE     A putative modular domain present in diverse signaling proteins
  JOURNAL   Cell 73 (4), 629-630 (1993)
   PUBMED   8500161
REFERENCE   10 (bases 1 to 10085)
  AUTHORS   Shearer,A.E., Hildebrand,M.S., Odell,A.M. and Smith,R.J.H.
  TITLE     Genetic Hearing Loss Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301607
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA790674.1, DQ228005.1,
            Z83844.5 and AW444985.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001039141.2.
            
            Summary: This gene encodes a protein with an N-terminal pleckstrin
            homology domain and a C-terminal coiled-coil region. The protein
            interacts with trio, which is involved with neural tissue
            development and controlling actin cytoskeleton organization, cell
            motility and cell growth. The protein also associates with F-actin
            and stabilizes F-actin structures. Mutations in this gene have been
            associated with a form of autosomal recessive nonsyndromic
            deafness. Multiple alternatively spliced transcript variants that
            would encode different isoforms have been found for this gene,
            however some transcripts may be subject to nonsense-mediated decay
            (NMD). [provided by RefSeq, Nov 2008].
            
            Transcript Variant: This variant (6) represents the longest
            transcript and encodes the longest protein (isoform 6).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: DQ228005.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA1965299,
                                    SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000644935.1/ ENSP00000496394.1
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-75                DA790674.1         61-135
            76-7577             DQ228005.1         1-7502
            7578-9707           Z83844.5           147999-150128
            9708-10085          AW444985.1         15-392              c
FEATURES             Location/Qualifiers
     source          1..10085
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q13.1"
     gene            1..10085
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /note="TRIO and F-actin binding protein"
                     /db_xref="GeneID:11078"
                     /db_xref="HGNC:HGNC:17009"
                     /db_xref="MIM:609761"
     exon            1..42
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            43..151
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            152..325
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     CDS             212..7309
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /note="isoform 6 is encoded by transcript variant 6;
                     tara-like protein; trio-associated repeat on actin;
                     protein Tara; TRF1-associated protein of 68 kDa"
                     /codon_start=1
                     /product="TRIO and F-actin-binding protein isoform 6"
                     /protein_id="NP_001034230.1"
                     /db_xref="CCDS:CCDS43015.1"
                     /db_xref="GeneID:11078"
                     /db_xref="HGNC:HGNC:17009"
                     /db_xref="MIM:609761"
                     /translation="MEEVPGDALCEHFEANILTQNRCQNCFHPEEAHGARYQELRSPS
                     GAEVPYCDLPRCPPAPEDPLSASTSGCQSVVDPGLRPGPKRGPSPSAGLPEEGPTAAP
                     RSRSRELEAVPYLEGLTTSLCGSCNEDPGSDPTSSPDSATPDDTSNSSSVDWDTVERQ
                     EEEAPSWDELAVMIPRRPREGPRADSSQRAPSLLTRSPVGGDAAGQKKEDTGGGGRSA
                     GQHWARLRGESGLSLERHRSTLTQASSMTPHSGPRSTTSQASPAQRDTAQAASTREIP
                     RASSPHRITQRDTSRASSTQQEISRASSTQQETSRASSTQEDTPRASSTQEDTPRASS
                     TQWNTPRASSPSRSTQLDNPRTSSTQQDNPQTSFPTCTPQRENPRTPCVQQDDPRASS
                     PNRTTQRENSRTSCAQRDNPKASRTSSPNRATRDNPRTSCAQRDNPRASSPSRATRDN
                     PTTSCAQRDNPRASRTSSPNRATRDNPRTSCAQRDNPRASSPSRATRDNPTTSCAQRD
                     NPRASRTSSPNRATRDNPRTSCAQRDNPRASSPNRAARDNPTTSCAQRDNPRASRTSS
                     PNRATRDNPRTSCAQRDNPRASSPNRATRDNPTTSCAQRDNPRASRTSSPNRATRDNP
                     RTSCAQRDNPRASSPNRTTQQDSPRTSCARRDDPRASSPNRTIQQENPRTSCALRDNP
                     RASSPSRTIQQENPRTSCAQRDDPRASSPNRTTQQENPRTSCARRDNPRASSRNRTIQ
                     RDNPRTSCAQRDNPRASSPNRTIQQENLRTSCTRQDNPRTSSPNRATRDNPRTSCAQR
                     DNLRASSPIRATQQDNPRTCIQQNIPRSSSTQQDNPKTSCTKRDNLRPTCTQRDRTQS
                     FSFQRDNPGTSSSQCCTQKENLRPSSPHRSTQWNNPRNSSPHRTNKDIPWASFPLRPT
                     QSDGPRTSSPSRSKQSEVPWASIALRPTQGDRPQTSSPSRPAQHDPPQSSFGPTQYNL
                     PSRATSSSHNPGHQSTSRTSSPVYPAAYGAPLTSPEPSQPPCAVCIGHRDAPRASSPP
                     RYLQHDPFPFFPEPRAPESEPPHHEPPYIPPAVCIGHRDAPRASSPPRHTQFDPFPFL
                     PDTSDAEHQCQSPQHEPLQLPAPVCIGYRDAPRASSPPRQAPEPSLLFQDLPRASTES
                     LVPSMDSLHECPHIPTPVCIGHRDAPSFSSPPRQAPEPSLFFQDPPGTSMESLAPSTD
                     SLHGSPVLIPQVCIGHRDAPRASSPPRHPPSDLAFLAPSPSPGSSGGSRGSAPPGETR
                     HNLEREEYTVLADLPPPRRLAQRQPGPQAQCSSGGRTHSPGRAEVERLFGQERRKSEA
                     AGAFQAQDEGRSQQPSQGQSQLLRRQSSPAPSRQVTMLPAKQAELTRRSQAEPPHPWS
                     PEKRPEGDRQLQGSPLPPRTSARTPERELRTQRPLESGQAGPRQPLGVWQSQEEPPGS
                     QGPHRHLERSWSSQEGGLGPGGWWGCGEPSLGAAKAPEGAWGGTSREYKESWGQPEAW
                     EEKPTHELPRELGKRSPLTSPPENWGGPAESSQSWHSGTPTAVGWGAEGACPYPRGSE
                     RRPELDWRDLLGLLRAPGEGVWARVPSLDWEGLLELLQARLPRKDPAGHRDDLARALG
                     PELGPPGTNDVPEQESHSQPEGWAEATPVNGHSPALQSQSPVQLPSPACTSTQWPKIK
                     VTRGPATATLAGLEQTGPLGSRSTAKGPSLPELQFQPEEPEESEPSRGQDPLTDQKQA
                     DSADKRPAEGKAGSPLKGRLVTSWRMPGDRPTLFNPFLLSLGVLRWRRPDLLNFKKGW
                     MSILDEPGEPPSPSLTTTSTSQWKKHWFVLTDSSLKYYRDSTAEEADELDGEIDLRSC
                     TDVTEYAVQRNYGFQIHTKDAVYTLSAMTSGIRRNWIEALRKTVRPTSAPDVTKLSDS
                     NKENALHSYSTQKGPLKAGEQRAGSEVISRGGPRKADGQRQALDYVELSPLTQASPQR
                     ARTPARTPDRLAKQEELERDLAQRSEERRKWFEATDSRTPEVPAGEGPRRGLGAPLTE
                     DQQNRLSEEIEKKWQELEKLPLRENKRVPLTALLNQSRGERRGPPSDGHEALEKEVQA
                     LRAQLEAWRLQGEAPQSALRSQEDGHIPPGYISQEACERSLAEMESSHQQVMEELQRH
                     HERELQRLQQEKEWLLAEETAATASAIEAMKKAYQEELSRELSKTRSLQQGPDGLRKQ
                     HQSDVEALKRELQVLSEQYSQKCLEIGALMRQAEEREHTLRRCQQEGQELLRHNQELH
                     GRLSEEIDQLRGFIASQGMGNGCGRSNERSSCELEVLLRVKENELQYLKKEVQCLRDE
                     LQMMQKDKRFTSGKYQDVYVELSHIKTRSEREIEQLKEHLRLAMAALQEKESMRNSLA
                     E"
     exon            326..465
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            466..667
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            668..839
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            840..4158
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            4159..4273
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            4274..5317
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            5318..5395
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            5396..5533
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            5534..5590
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            5591..5698
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            5699..5788
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            5789..5898
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            5899..6424
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            6425..6535
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            6536..6683
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            6684..6786
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            6787..6946
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            6947..7060
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            7061..7147
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            7148..7311
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     exon            7312..10085
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8020..8025
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /note="hexamer: AATAAA"
     polyA_site      8038
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /note="major polyA site"
     polyA_site      8070
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
     regulatory      10029..10034
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /note="hexamer: AATAAA"
     regulatory      10036..10041
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /note="hexamer: AATACA"
     polyA_site      10055
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
     regulatory      10064..10069
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
                     /note="hexamer: AATAAA"
     polyA_site      10065
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
     polyA_site      10085
                     /gene="TRIOBP"
                     /gene_synonym="DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA"
ORIGIN      
        1 actcccaccc cggcagcccc tgtgcgtgga aacccagcca aggtctgatg atggaggagc
       61 cttggccctg gctgtcccag gggaggaggt gaaattcctc agctctccac caagattggc
      121 cacaaaagcc tgatcccctg gaacacagca ggcctcacat agacggtcag ccattggatc
      181 ataggaactg ccctggcctg actcacccaa tatggaggag gtgcctgggg atgccctgtg
      241 tgaacacttt gaggccaaca tacttaccca gaaccgctgt caaaactgct tccaccctga
      301 ggaggcccat ggagcaagat accaggagct caggagccct tcaggtgctg aggtgcccta
      361 ctgcgacctg cctcgatgtc cacctgcccc tgaggaccca ctcagcgcct caacctccgg
      421 ctgccagtct gtggtggacc caggcctcag gccagggccc aagaggggcc catccccctc
      481 agcagggctc ccagaagagg gtcccacagc tgcccccagg agcaggagcc gggagcttga
      541 ggcagtaccc tatctggagg gcctgaccac ttccttgtgt ggcagctgca acgaggaccc
      601 cggctctgac cccacctcca gccctgactc cgccacccct gatgatacca gcaactcgtc
      661 ctctgtggac tgggacactg ttgagaggca ggaggaggag gcccccagct gggacgagct
      721 cgcagtgatg atcccgagga ggcctcggga ggggccgaga gctgacagct cccaaagggc
      781 tccgtctctc ctcaccaggt cccctgtggg aggagatgct gcaggccaga aaaaggagga
      841 caccggcggt gggggccgga gcgcaggaca gcactgggca aggctccggg gagaaagcgg
      901 gttgtccctg gagcggcacc ggtcaacact gacccaggct tcctccatga caccacacag
      961 tggacctcga agcaccacgt ctcaggcttc tcctgcccaa agggacactg ctcaggctgc
     1021 ctctacacgt gaaatcccca gagcctcctc tccccatcga atcacccaaa gggacacctc
     1081 cagggcctca tccacccaac aggaaatctc cagggcctca tccacccaac aggaaacctc
     1141 cagggcctca tccacccaag aggacacccc tagggcctca tccacccaag aggacacccc
     1201 cagggcctca tctacacagt ggaacacccc cagagcttcc tctccctcac gaagcaccca
     1261 actggataac cccagaacct cttctaccca gcaggacaac ccccaaactt cttttcctac
     1321 ttgtactccc cagcgggaaa accccaggac accctgtgtc cagcaggacg atcccagagc
     1381 ctcctctccc aacagaacca ctcaacgaga gaattccaga acatcctgtg cccagcggga
     1441 caatcccaaa gcctccagaa cctcctctcc caatagagcc acacgagaca accccagaac
     1501 atcctgcgcc cagcgggaca atcccagagc ctcctctccc agtagagcta cacgagacaa
     1561 ccccacaaca tcctgtgccc agcgggacaa tcccagagcc tccagaacct cctctcccaa
     1621 tagagccaca cgagacaacc ccagaacatc ctgtgcccag cgggacaatc ccagagcctc
     1681 ctctcccagt agagctacac gagacaaccc cacaacatcc tgtgcccagc gggacaatcc
     1741 cagagcctcc agaacctcct ctcccaatag agccacacga gacaacccca gaacatcctg
     1801 cgcccagcgg gacaatccca gagcctcctc tcccaataga gctgcacgag acaaccccac
     1861 aacatcctgt gcccagcggg acaatcccag agcctccaga acctcctctc ccaatagagc
     1921 cacacgagac aaccccagaa catcctgtgc ccagcgggac aatcccagag cctcctctcc
     1981 caatagagct acacgagaca accccacaac atcctgtgcc cagcgggaca atcccagagc
     2041 ctccagaacc tcctctccca atagagccac acgagataac cccagaacat cctgtgccca
     2101 gcgggacaat cccagagcct cctctcccaa cagaaccacc caacaagaca gccccagaac
     2161 atcctgtgcc cgacgggacg atcccagagc ctcctctcct aacagaacca tccaacaaga
     2221 gaaccccaga acatcctgtg ccctacggga caatcccaga gcctcctctc ccagcagaac
     2281 catccaacaa gagaacccca gaacatcctg tgcccaacgg gacgatccca gagcctcctc
     2341 tcctaacaga accacccaac aagagaaccc cagaacatcc tgtgcccgac gggacaatcc
     2401 cagagcctcc tctcgcaaca gaaccatcca gcgagacaac cccagaacat cctgtgccca
     2461 gcgggacaat cccagagcct cctctcctaa cagaaccatc caacaagaga acctcagaac
     2521 atcctgtacc cgacaggaca atcccaggac ctcctctccc aatagagcca cacgagacaa
     2581 ccccagaaca tcctgtgccc agcgggacaa tctcagagcc tcctctccca tcagagccac
     2641 ccaacaggac aaccccagaa cttgtattca acagaacatc cccagatcat cttctaccca
     2701 acaagacaac cctaaaacct cttgtaccaa acgagataac ctcagaccca cttgtacaca
     2761 gcgggaccgc acacagtcct tttcctttca acgagacaac cctggaacct cctcatctca
     2821 atgctgcacc caaaaggaga atctgagacc atcatctccc caccgctcca ctcaatggaa
     2881 caatcccagg aattcatctc cccatcgtac taacaaagac atcccctggg cctcgtttcc
     2941 cctccggcca actcagagtg atggtccccg aacctcttcc ccatctcgct ccaagcaaag
     3001 cgaggttccc tgggcatcca tcgccctccg gccaacccaa ggtgacaggc ctcagacatc
     3061 ctctcccagc aggccagccc agcatgaccc accccagtcc tcctttggcc ccacccagta
     3121 caacttgcca tcccgggcca cctcttcctc ccataaccca ggccaccaga gcacctcccg
     3181 aacttcctca cctgtgtacc ccgctgccta tggggctccc ctgacctctc ctgagccctc
     3241 ccagcctcca tgtgctgtgt gcattgggca ccgggatgcc cctcgagcct cttcgccccc
     3301 tcgctatttg cagcacgacc ccttcccctt cttcccagag ccccgcgccc ctgagagtga
     3361 accgccccac cacgagcctc cctatatacc acctgctgtg tgcattggac accgagatgc
     3421 cccccgggcg tcctcgcccc cccgccacac ccaatttgac cccttcccct tcctcccaga
     3481 cacatcagat gccgagcatc agtgtcagtc cccccaacac gagccccttc agctccctgc
     3541 acctgtgtgt attgggtacc gagatgcacc ccgggcctcc tccccaccac gccaggcccc
     3601 agagccttcc ctcttattcc aggacctccc cagggccagc acagagagcc ttgtcccttc
     3661 catggactct ctgcacgagt gcccccacat ccccacccct gtgtgcattg ggcaccggga
     3721 tgcaccctcc ttctcatccc caccacgcca ggctcctgag ccatccctct tcttccagga
     3781 tccccctgga actagtatgg agagcctggc cccctccact gactctctgc atggctcccc
     3841 agtgctgatc ccccaagtgt gcatcgggca ccgggatgca ccccgagcct cctccccacc
     3901 ccgccaccca cccagtgacc tagcgttcct ggcaccctca ccttcaccgg gcagctctgg
     3961 gggctcccgg ggctcagcgc ctcccgggga gaccaggcac aacttggagc gggaggagta
     4021 cactgtgctg gccgacctgc ccccacccag gaggctggcc cagagacagc cagggcccca
     4081 ggcgcagtgc agcagcgggg gccgcaccca cagccctggc cgtgcagagg tggagcgcct
     4141 cttcgggcaa gagcgcagga agtccgaggc agcgggggcc ttccaggccc aggacgaggg
     4201 acggtcacag cagcccagcc aaggccagag ccaacttctc cgaagacagt ccagccctgc
     4261 ccccagcagg caggtgacca tgctccctgc caaacaggca gaactgaccc ggcggagcca
     4321 agcagagccc cctcatcctt ggagtcctga gaagagacct gagggagatc ggcagctcca
     4381 ggggtccccg ctgcccccca ggacatcagc caggacccct gagagggagc tgcggacaca
     4441 gagacctctg gagagtggcc aagcaggccc aagacagcct ctgggggtgt ggcagagtca
     4501 ggaggaaccg ccagggtccc agggccctca tagacaccta gaaaggagct ggagcagcca
     4561 ggagggaggc ctgggccctg ggggctggtg gggatgtgga gagcccagcc tgggggcagc
     4621 caaagccccg gagggagcat gggggggcac ttccagggag tacaaggaga gctgggggca
     4681 gccagaggcc tgggaggaga agcccactca tgagctcccc agagaactag gaaagagaag
     4741 cccactcacg agcccccctg agaactgggg aggccccgca gagtcctcac aatcctggca
     4801 ctctgggaca cccactgctg tgggctgggg ggcagaggga gcgtgtccat acccgcgtgg
     4861 ctctgagagg cgacccgagc ttgactggag ggatctgctt ggccttctcc gggcaccagg
     4921 agagggggtc tgggcccgtg tccccagcct ggactgggag ggcctcttgg agctcctgca
     4981 ggccaggctg ccccgcaagg acccagctgg acacagggat gacctggcca gggctttagg
     5041 gccagagctg ggtcccccag gcacaaacga tgtccctgag caggagtcac acagccagcc
     5101 agaaggctgg gccgaggcca ccccagtcaa tggacacagc cccgcactgc agtcccagag
     5161 cccggtccag ctgcccagcc ctgcctgcac ctccacccag tggccaaaga tcaaagtgac
     5221 aagaggacca gcgaccgcaa ctctggcagg cctggagcag acgggccccc tggggagcag
     5281 gagcactgcg aagggcccca gcttgccaga gctgcagttc caaccagagg agcctgagga
     5341 gtcagaacca agcagaggcc aagaccccct gactgaccag aagcaggcag actcggcaga
     5401 caagaggcca gcagagggca aggctgggag cccgctcaag ggccgactgg tgacctcatg
     5461 gcggatgccc ggggaccggc ccacgctgtt caatccgttc ctgctgtctc tgggggtcct
     5521 caggtggcga aggcccgatc tgctcaactt caagaaggga tggatgtcga tcttggacga
     5581 gcctggagag cctccctccc cctcgctcac caccacctct acttcgcagt ggaagaaaca
     5641 ttggtttgtg ctgacagatt caagtctcaa atattacaga gactccactg ctgaggaggc
     5701 agatgagctg gatggtgaga tcgacctgcg ttcctgcacg gatgtcactg agtacgcggt
     5761 gcagcgcaac tatggcttcc agatccacac caaggatgct gtctatacct tgtcggccat
     5821 gacctcaggc atccggcgga actggatcga ggctctgaga aagaccgtac gtccaacttc
     5881 agccccagat gtcaccaagc tctcggactc taacaaggag aacgcgctgc acagctacag
     5941 cacccagaag ggccccctga aggcagggga gcagcgggcg ggctctgagg tcatcagccg
     6001 gggtggccct cggaaggcgg acgggcagcg tcaggccttg gactacgtgg agctctcgcc
     6061 gctgacccag gcttccccgc agcgggcccg caccccagcc cgcactcctg accgcctggc
     6121 caagcaggag gagctggagc gggacctggc ccagcgctcc gaggagcggc gcaagtggtt
     6181 tgaggccaca gacagcagga ccccagaggt gcctgctggt gaggggccgc gccggggcct
     6241 gggtgccccc ctgactgagg accagcaaaa ccggcttagt gaggagatcg agaagaagtg
     6301 gcaggagctg gagaagctgc ccctgcggga gaataagcgg gtgcccctca ctgccctgct
     6361 caaccaaagc cgcggagagc gccgagggcc cccaagtgac ggccacgagg cactggagaa
     6421 ggaggttcag gctcttcggg cccagctgga ggcgtggcgt ctccaagggg aggctcctca
     6481 gagtgcactg agatcccagg aggatggcca catccccccg ggctacatct cacaggaggc
     6541 atgtgagcgc agcctggcag agatggagtc ctcgcaccag caggtgatgg aggagctgca
     6601 gcggcaccac gagcgggagc tgcagcgcct gcagcaggag aaggagtggc tcctggctga
     6661 ggagacggca gccacggcct cagccattga agccatgaag aaggcctacc aggaagagct
     6721 gagccgagag ctgagcaaaa cacggagtct ccagcagggc ccggatggcc tccggaagca
     6781 gcaccagtca gatgtggagg cactgaagcg agagctgcag gtgctatcgg agcagtactc
     6841 gcagaagtgc ctggagattg gggcactcat gcggcaggct gaggagcgcg agcacacgct
     6901 gcgccgctgc cagcaggagg gccaggagct gctgcgccac aaccaggagc tgcatggccg
     6961 cctgtcagag gagatagacc agctgcgcgg cttcattgcc tcgcagggca tgggcaatgg
     7021 ctgcgggcgc agcaacgagc ggagttcctg cgagctagag gtgctgcttc gcgtaaaaga
     7081 aaacgaactc cagtacctaa agaaggaggt gcagtgcctc cgggacgagc tccagatgat
     7141 gcagaaggac aagcgcttca cctcgggaaa gtaccaggac gtctatgtgg agctgagcca
     7201 catcaagaca cggtctgagc gggagatcga gcagctgaag gagcacctgc gtcttgccat
     7261 ggccgccctc caggagaagg agtcgatgcg caacagcctg gctgagtaga ggtcccgccc
     7321 agctgcagac cctccaggct ggaggaccag ccgccctcct tccctcctgg atggaagtaa
     7381 aaagccaagc tttctcccca ccctctgtgg gccacacgtg cacttgcacc caccacacac
     7441 acacacacac acacacacac acacacagac acacagacac atacgcacac acgtgcacac
     7501 atgtacacac ggatacacac acacacacac acactgcata tctgagcgcg cccctcgcac
     7561 tgggtctcac cttgcacctt cttcaggatt ttatatgtga agagattttt atatagattt
     7621 ttttcctttt tttccaaaac actttatact ttaaaaaaaa aaaaaaaaag caattcctgg
     7681 tggctgtgtg cctccaaccc tggtccccct ctgtctccag ccaccctctg cttgggcttc
     7741 tgagctggtg gccctggccc agaggtctgg cggaggccca ggcagcagcc atggcggggt
     7801 gtctctacag gggagaggcg ggagcctgcc accctcttcc tgccctacct cctactaaca
     7861 cttcctgccc catttggacc cgtaccatgg ggctcaggac agagggagct agcagctggc
     7921 ctccatggcc ccacagcctc cttcgaggct gtgctgggtg cagaaccgcc agagccaccc
     7981 aaaaggtgtt tctcttctgc tccctgaacc tcttaactta ataaaacgtt ccagcagctc
     8041 tggtgtccta gatggctggc agaacaggaa tggagattgg ggtttcttga agtggcttcc
     8101 ccactcacac cctacccaca ccccaccctt cctaaagcag cggcctctag gcttctgagg
     8161 gtggggctga aaatccaagg tctcccttag tacagactgt gacgccccca gtgtggcttg
     8221 caggctagtg gcagcggaag catgtgggaa atagcaagtc cagtcccacc ccaacctact
     8281 gaaccagcgt ctgcatttta acagggtgcc ccagtgattc acgtgacctt caaagtctga
     8341 ggagccctgg agtagggccc agggcccagg cctgagagag aggcctgggg ctaagactag
     8401 ccctgaccat gaccttgggc aagtcacacc cctcactgag cctcagtttg cttctccatc
     8461 aaatgggtgt ttttctttca gtcccagtcc tctcctgagg cttttcccag aggtcatgcc
     8521 cagccctggg caggggtgag ggcagggttg tggctgccag gcacaggtcc agcgggctgg
     8581 gagtcctcac catggctggc tgtgctgatt atggttgcat ggggctggga agaggtccca
     8641 tttctgagcc acagaaacag gccacatcca gtaggggtcc tggaaggctg tgagaaccca
     8701 gaggagaccc tgacccagcc tgcaggtaga agactgcctt tggaggcagc tttgaaagat
     8761 gactcttcca gatggaagaa tcaggaaggg ccttcccacg tggaggcacc agcaggagca
     8821 aacgtccctg gtgtgttcag gcaaagagcc acaggagagt gacctcgtgg agtgggaaga
     8881 gattgtttcc cattcccttg ccagggattg gttgaggaat ggacaaggct tgcagttcac
     8941 gcaagccagt gaaatgcgag gggaagtctt gggagtgggg tggaggaggt catctgggaa
     9001 gggtgtcctt ggtcttaaaa agaggcacaa gaggccgggc gcggtggctc ttgcctatta
     9061 tcctagcact ttgggaggcc aaggcagggg gatcacttga ggtcaggagt tcgagaccag
     9121 cctggccaac atggtgaaac cccgtctcta ctaaaaatac aaaaaattag ccagacgtgg
     9181 tgccaagcac ctgtaatccc agctactcag gaggctgagg caggagaatc acttgaaccc
     9241 aggaggcaga agttgcagtg agccaagatc gcgccactgc actccagcct gtgtgacaag
     9301 ggcgaaactc catctcaaaa aaaaaggggg agggtggcgc aaggagagga cacctctctc
     9361 ctggccctgt cgccatcttc cctcatcaat agctaaaaat ggcaaagaag aaagagccag
     9421 gaccccgtgc catcaaatcc tccggccttg tgctgccctg tctcggcact gcttgcctta
     9481 ggagatcagg agtgttccta gccactggga gatgggatgc ctgccactgg cagctctgcc
     9541 gtccatcctc accatcccac cgtcccccca agacagctct ccatcccatc ctctgcctcc
     9601 cttctagcct caacctaact cagggctccc ccgtctcaca cctgggttgc aggaacatcc
     9661 tcttcaccaa cccccctccc atccctcctc agggctgcct agggcagcta caaaaaccag
     9721 gccagcctct gaaaggtgct ccacatctca ttaaaaaccg tgaaatcgca ctgccccact
     9781 atcagaatag ctcagaggag aaggtctgcc agtaccaagt gctggtgggg atacggagaa
     9841 gagcagctcc caggcgctgc tggtgcaaat gtgctttgtt gcacccacgg caggaaactg
     9901 accagcttct catcaagtta aatacgcatc taccctatgg cctagcattt ccactcttgc
     9961 ttacacatcc aggagaaatg agtccttaca tgcacaaaag gatgagtacc gtaatactca
    10021 catcagcaaa taaacaatac agctgtgtgt cagtagtaga atgaataaat tgtggtcgat
    10081 tcata
//
