LOCUS       NM_001039111            8704 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens tripartite motif containing 71 (TRIM71), mRNA.
ACCESSION   NM_001039111 XM_067369
VERSION     NM_001039111.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Yuan,J., Lv,T., Yang,J., Wu,Z., Yan,L., Yang,J., Shi,Y. and
            Jiang,L.
  TITLE     HDLBP Promotes Hepatocellular Carcinoma Proliferation and Sorafenib
            Resistance by Suppressing Trim71-dependent RAF1 Degradation
  JOURNAL   Cell Mol Gastroenterol Hepatol 15 (2), 307-325 (2023)
   PUBMED   36244648
  REMARK    GeneRIF: HDLBP Promotes Hepatocellular Carcinoma Proliferation and
            Sorafenib Resistance by Suppressing Trim71-dependent RAF1
            Degradation.
REFERENCE   2  (bases 1 to 8704)
  AUTHORS   Luptak,J., Mallery,D.L., Jahun,A.S., Albecka,A., Clift,D.,
            Ather,O., Slodkowicz,G., Goodfellow,I. and James,L.C.
  TITLE     TRIM7 Restricts Coxsackievirus and Norovirus Infection by Detecting
            the C-Terminal Glutamine Generated by 3C Protease Processing
  JOURNAL   Viruses 14 (8), 1610 (2022)
   PUBMED   35893676
  REMARK    GeneRIF: TRIM7 Restricts Coxsackievirus and Norovirus Infection by
            Detecting the C-Terminal Glutamine Generated by 3C Protease
            Processing.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8704)
  AUTHORS   Foster,D.J., Chang,H.M., Haswell,J.R., Gregory,R.I. and Slack,F.J.
  TITLE     TRIM71 binds to IMP1 and is capable of positive and negative
            regulation of target RNAs
  JOURNAL   Cell Cycle 19 (18), 2314-2326 (2020)
   PUBMED   32816599
  REMARK    GeneRIF: TRIM71 binds to IMP1 and is capable of positive and
            negative regulation of target RNAs.
REFERENCE   4  (bases 1 to 8704)
  AUTHORS   Torres-Fernandez,L.A., Jux,B., Bille,M., Port,Y., Schneider,K.,
            Geyer,M., Mayer,G. and Kolanus,W.
  TITLE     The mRNA repressor TRIM71 cooperates with Nonsense-Mediated Decay
            factors to destabilize the mRNA of CDKN1A/p21
  JOURNAL   Nucleic Acids Res 47 (22), 11861-11879 (2019)
   PUBMED   31732746
  REMARK    GeneRIF: TRIM71-mediated target recognition and repression
            mechanisms uncovers a role for this stem cell-specific factor and
            oncogene in non-canonical Nonsense-mediated Decay (NMD), revealing
            the existence of a novel mRNA surveillance mechanism which has been
            termed here the TRIM71/NMD axis.
REFERENCE   5  (bases 1 to 8704)
  AUTHORS   Chen,Y., Hao,Q., Wang,J., Li,J., Huang,C., Zhang,Y., Wu,X., Lu,H.
            and Zhou,X.
  TITLE     Ubiquitin ligase TRIM71 suppresses ovarian tumorigenesis by
            degrading mutant p53
  JOURNAL   Cell Death Dis 10 (10), 737 (2019)
   PUBMED   31570706
  REMARK    GeneRIF: Ubiquitin ligase TRIM71 suppresses ovarian tumorigenesis
            by degrading mutant p53.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8704)
  AUTHORS   Wulczyn,F.G., Cuevas,E., Franzoni,E. and Rybak,A.
  TITLE     MiRNA need a TRIM regulation of miRNA activity by Trim-NHL proteins
  JOURNAL   Adv Exp Med Biol 700, 85-105 (2010)
   PUBMED   21627033
  REMARK    Review article
REFERENCE   7  (bases 1 to 8704)
  AUTHORS   Lin,Y.C., Hsieh,L.C., Kuo,M.W., Yu,J., Kuo,H.H., Lo,W.L., Lin,R.J.,
            Yu,A.L. and Li,W.H.
  TITLE     Human TRIM71 and its nematode homologue are targets of let-7
            microRNA and its zebrafish orthologue is essential for development
  JOURNAL   Mol Biol Evol 24 (11), 2525-2534 (2007)
   PUBMED   17890240
  REMARK    GeneRIF: Repression of human TRIM71 and the zebrafish lin-41
            ortholog was abolished when predicted let-7 target sites were
            mutated. Regulation of TRIM71 expression by let-7 seems to have
            been evolutionarily conserved.
REFERENCE   8  (bases 1 to 8704)
  AUTHORS   Schulman,B.R., Esquela-Kerscher,A. and Slack,F.J.
  TITLE     Reciprocal expression of lin-41 and the microRNAs let-7 and mir-125
            during mouse embryogenesis
  JOURNAL   Dev Dyn 234 (4), 1046-1054 (2005)
   PUBMED   16247770
REFERENCE   9  (bases 1 to 8704)
  AUTHORS   Lancman,J.J., Caruccio,N.C., Harfe,B.D., Pasquinelli,A.E.,
            Schageman,J.J., Pertsemlidis,A. and Fallon,J.F.
  TITLE     Analysis of the regulation of lin-41 during chick and mouse limb
            development
  JOURNAL   Dev Dyn 234 (4), 948-960 (2005)
   PUBMED   16245339
REFERENCE   10 (bases 1 to 8704)
  AUTHORS   Lee,Y.S., Kim,H.K., Chung,S., Kim,K.S. and Dutta,A.
  TITLE     Depletion of human micro-RNA miR-125b reveals that it is critical
            for the proliferation of differentiated cells but not for the
            down-regulation of putative targets during differentiation
  JOURNAL   J Biol Chem 280 (17), 16635-16641 (2005)
   PUBMED   15722555
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC139452.4, DQ232881.1 and
            AW196612.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001039111.2.
            
            Summary: The protein encoded by this gene is an E3
            ubiquitin-protein ligase that binds with miRNAs and maintains the
            growth and upkeep of embryonic stem cells. This gene also is
            involved in the G1-S phase transition of the cell cycle. [provided
            by RefSeq, Dec 2015].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ232881.1, DT932675.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2145743
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000383763.6/ ENSP00000373272.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                AC139452.4         115068-115088       c
            22-3159             DQ232881.1         1-3138
            3160-8228           AC139452.4         35737-40805         c
            8229-8704           AW196612.1         12-487              c
FEATURES             Location/Qualifiers
     source          1..8704
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p22.3"
     gene            1..8704
                     /gene="TRIM71"
                     /gene_synonym="HYC4; HYDCC1; LIN-41; LIN41"
                     /note="tripartite motif containing 71"
                     /db_xref="GeneID:131405"
                     /db_xref="HGNC:HGNC:32669"
                     /db_xref="MIM:618570"
     exon            1..936
                     /gene="TRIM71"
                     /gene_synonym="HYC4; HYDCC1; LIN-41; LIN41"
                     /inference="alignment:Splign:2.1.0"
     CDS             85..2691
                     /gene="TRIM71"
                     /gene_synonym="HYC4; HYDCC1; LIN-41; LIN41"
                     /EC_number="2.3.2.27"
                     /note="homolog of C. elegans Lin-41; tripartite
                     motif-containing protein 71; abnormal cell LINeage LIN-41;
                     protein lin-41 homolog; tripartite motif containing 71, E3
                     ubiquitin protein ligase; RING-type E3 ubiquitin
                     transferase TRIM71"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase TRIM71"
                     /protein_id="NP_001034200.1"
                     /db_xref="CCDS:CCDS43060.1"
                     /db_xref="GeneID:131405"
                     /db_xref="HGNC:HGNC:32669"
                     /db_xref="MIM:618570"
                     /translation="MASFPETDFQICLLCKEMCGSPAPLSSNSSASSSSSQTSTSSGG
                     GGGGPGAAARRLHVLPCLHAFCRPCLEAHRLPAAGGGAAGEPLKLRCPVCDQKVVLAE
                     AAGMDALPSSAFLLSNLLDAVVATADEPPPKNGRAGAPAGAGGHSNHRHHAHHAHPRA
                     SASAPPLPQAPQPPAPSRSAPGGPAASPSALLLRRPHGCSSCDEGNAASSRCLDCQEH
                     LCDNCVRAHQRVRLTKDHYIERGPPGPGAAAAAQQLGLGPPFPGPPFSILSVFPERLG
                     FCQHHDDEVLHLYCDTCSVPICRECTMGRHGGHSFIYLQEALQDSRALTIQLLADAQQ
                     GRQAIQLSIEQAQTVAEQVEMKAKVVQSEVKAVTARHKKALEERECELLWKVEKIRQV
                     KAKSLYLQVEKLRQNLNKLESTISAVQQVLEEGRALDILLARDRMLAQVQELKTVRSL
                     LQPQEDDRVMFTPPDQALYLAIKSFGFVSSGAFAPLTKATGDGLKRALQGKVASFTVI
                     GYDHDGEPRLSGGDLMSAVVLGPDGNLFGAEVSDQQNGTYVVSYRPQLEGEHLVSVTL
                     CNQHIENSPFKVVVKSGRSYVGIGLPGLSFGSEGDSDGKLCRPWGVSVDKEGYIIVAD
                     RSNNRIQVFKPCGAFHHKFGTLGSRPGQFDRPAGVACDASRRIVVADKDNHRIQIFTF
                     EGQFLLKFGEKGTKNGQFNYPWDVAVNSEGKILVSDTRNHRIQLFGPDGVFLNKYGFE
                     GALWKHFDSPRGVAFNHEGHLVVTDFNNHRLLVIHPDCQSARFLGSEGTGNGQFLRPQ
                     GVAVDQEGRIIVADSRNHRVQMFESNGSFLCKFGAQGSGFGQMDRPSGIAITPDGMIV
                     VVDFGNNRILVF"
     exon            937..1104
                     /gene="TRIM71"
                     /gene_synonym="HYC4; HYDCC1; LIN-41; LIN41"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1239
                     /gene="TRIM71"
                     /gene_synonym="HYC4; HYDCC1; LIN-41; LIN41"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..8704
                     /gene="TRIM71"
                     /gene_synonym="HYC4; HYDCC1; LIN-41; LIN41"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8681..8686
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRIM71"
                     /gene_synonym="HYC4; HYDCC1; LIN-41; LIN41"
                     /note="hexamer: AATAAA"
     polyA_site      8704
                     /gene="TRIM71"
                     /gene_synonym="HYC4; HYDCC1; LIN-41; LIN41"
                     /note="major polyA site"
ORIGIN      
        1 actcccccac ccacctcgtc cgctctctcc tcctcctcct cctcttcctc tctggtctcc
       61 tccctcctcc gggctgggtt gcaaatggct tcgttccccg agaccgattt ccagatctgc
      121 ttgctgtgca aggagatgtg cggctcgccg gcgccgctct cctccaactc gtccgcgtcg
      181 tcgtcctcct cgcagacgtc cacgtcgtcg gggggcggcg gcgggggccc tggggcggcg
      241 gcgcgccgcc tacacgtcct gccctgcctg cacgccttct gccgcccctg cctcgaggcg
      301 caccggctgc cggcggcggg cggcggcgcg gcgggagagc cgctcaagct gcgctgcccc
      361 gtgtgcgacc agaaagtagt gctagccgag gcggcgggta tggacgcgct gccttcgtcc
      421 gccttcctgc ttagcaacct gctcgacgcg gtggtggcca ctgccgacga gccgccgccc
      481 aagaacgggc gcgccggcgc tccggcggga gcgggcggcc acagcaacca ccggcaccac
      541 gctcaccacg cgcacccgcg cgcgtccgcc tccgcgccgc cactcccgca ggcgccgcag
      601 ccgcccgcgc cttcccgctc ggcacccggc ggccctgccg cttccccgtc ggcgctgctg
      661 ctccgccgtc ctcacggctg cagctcgtgc gatgagggca acgcagcttc ttcgcgctgc
      721 ctcgactgcc aggagcacct gtgcgacaac tgcgtccgag cgcaccagcg cgtgcgcctc
      781 accaaggacc actacatcga gcgcggcccg ccgggtcccg gtgccgcagc agcggcgcag
      841 cagctcgggc tcgggccgcc ctttcccggc ccgcccttct ccatcctctc agtgtttccc
      901 gagcgcctcg gcttctgcca gcaccacgac gacgaggtgc tgcacctgta ctgtgacact
      961 tgctctgtac ccatctgtcg tgagtgcaca atgggccggc atgggggcca cagcttcatc
     1021 tacctccagg aggcactgca ggactcacgg gcactcacca tccagctgct ggcagatgcc
     1081 cagcagggac gacaggcaat ccagctgagc atcgagcagg cccagacggt ggcggaacag
     1141 gtggagatga aggcgaaggt tgtgcagtcg gaggtcaaag ccgtgacggc gaggcataag
     1201 aaagccctgg aggaacgcga gtgtgagctg ctgtggaagg tagaaaagat ccgccaggtg
     1261 aaagccaagt ctctgtacct gcaggtggag aagctgcggc aaaacctcaa caagcttgag
     1321 agcaccatca gtgccgtgca gcaggtcctg gaggagggta gagcgctaga catcctactg
     1381 gcccgagacc ggatgctggc ccaggtgcag gagctgaaga ccgtgcggag cctcctgcag
     1441 ccccaggaag acgaccgagt catgttcaca ccccccgatc aggcactgta ccttgccatc
     1501 aagtcttttg gctttgttag cagcggggcc tttgccccac tcaccaaggc cacaggcgat
     1561 ggcctcaagc gtgccctcca gggtaaggtg gcctccttca cagtcattgg ttatgaccac
     1621 gatggtgagc cccgcctctc aggaggcgac ctgatgtcgg ctgtggtcct gggccctgat
     1681 ggcaacctgt ttggtgcaga ggtgagtgat cagcagaatg ggacatacgt ggtgagttac
     1741 cgaccccagc tggagggtga gcacctggta tctgtgacac tgtgcaacca gcacattgag
     1801 aacagccctt tcaaggtggt ggtcaagtca ggccgcagct acgtgggcat tgggctcccg
     1861 ggcctgagct tcggcagtga gggtgacagc gatggcaagc tctgccgccc ttggggtgtg
     1921 agtgtagaca aggagggcta catcattgtc gccgaccgca gcaacaaccg catccaggtg
     1981 ttcaagccct gcggcgcctt ccaccacaaa ttcggcaccc tgggctcccg gcctgggcag
     2041 ttcgaccgac cagccggcgt ggcctgtgac gcctcacgca ggatcgtggt ggctgacaag
     2101 gacaatcatc gcatccagat cttcacgttc gagggccagt tcctcctcaa gtttggtgag
     2161 aaaggaacca agaatgggca gttcaactac ccttgggatg tggcggtgaa ttctgagggc
     2221 aagatcctgg tctcagacac gaggaaccac cggatccagc tgtttgggcc tgatggtgtc
     2281 ttcctaaaca agtatggctt cgagggggct ctctggaagc actttgactc cccacggggt
     2341 gtggccttca accatgaggg ccacttggtg gtcactgact tcaacaacca ccggctcctg
     2401 gttattcacc ccgactgcca gtcggcacgc tttctgggct cggagggcac aggcaatggg
     2461 cagttcctgc gcccacaagg ggtagctgtg gaccaggaag ggcgcatcat tgtggcggat
     2521 tccaggaacc atcgggtaca gatgtttgaa tccaacggca gcttcctgtg caagtttggt
     2581 gctcaaggca gcggctttgg gcagatggac cgcccttccg gcatcgccat cacccccgac
     2641 ggaatgatcg ttgtggtgga ctttggcaac aatcgaatcc tcgtcttcta attgcatttc
     2701 ctaggtttct gtgtttgggg tgtgtgtgcg tgtctctctc tctctctctc tctttctctt
     2761 tctctctctt tttgaatttc aaagaagaaa cagtctcagg gaaatttctt ttttcttttt
     2821 tttttttaaa gagaacaaga aaagtacaac attgcttaag tcctacctca tctttatttt
     2881 tttacagatg aatgtactta tcttttctgc agggattgag cctgtgaagt gataatttct
     2941 atctacctca taaatcttta catttccttc tgcaacaggc cctcttcccc tcctcagtgg
     3001 agtttgcatt tccctcttcc cctgcgtggg gcatgatatg cacaagcctg gcatctgtat
     3061 ggctgggagg gcactggatg tgtgtggtgg ggtgtattct gtagattgag ccaaggaaac
     3121 acaaaaaaaa actactaagt aaaaaaacaa aaaactataa aacatggaaa aaataggatt
     3181 tgaaatgcat aattatagaa tacctgtgtt cttgagaata ctgtttatat ggggtttaga
     3241 ttatgttgtg ttgttttgat ctttttggaa aatcttctct ttttaaatgc tgcaacagag
     3301 aaatttcctc tgttctctgt ttatacctca gtgtgtttaa catcctcttc tgcatcgttt
     3361 cttgatctta gacgttgtgg ctgtggcttg ctagccaaga aaagcagacc cttcatattt
     3421 tagggtatat aatctttgtt tcttttaagg gagggtgtgt gcatgtttgt ttcaaagcca
     3481 tcttgcaccc aagtagtaaa gctgaaaatg acacactgcc tcccaaagaa cctctccttt
     3541 tcacactttg gtttcttaaa aaagtatata gatggtagct caggattttt gattaaacat
     3601 attctcaaaa gttatgcttt cttattttgc tgttaggtta taaagggtta aatggaagga
     3661 aaaaggaaat taacctcgtt ctcagagaga gggttttcag gcatgggctg acgtggacat
     3721 gaatgtgggt ggacatgcgt gtgctgaggg agtcagggag cacattaaag aacaaggcac
     3781 ccccaaacta taggaactcc acagaaaggg cagggtctgc cctgacccgc gagtgcatgt
     3841 tgttctgtag tgctaggggt ttcttttcct aagaagagta ggcaaaggaa agaactttta
     3901 ttttacaagc acaatttttt cctgctttga aagttcaggg aaatagaagg tttgaaagtc
     3961 aaattaatac caacagccca gtcccacacc ctctgcttgg gtcacttttt ataggattgt
     4021 ttgcacccag gccatgcttt aggacctcat ggttaacatg aatccccccg ccccctctcc
     4081 ccccaaggcc tttgaccata aaggatctag aaaccaaatt gtcccaacct gaaatgtagg
     4141 atgtaatgtc ttttcttaaa cctgtaactc ggtaccaaag aatgaaaatt taaggctccc
     4201 actgcagaac tgtggggtag aatctgtgtc actttacagg attggttggt tgtaagctgg
     4261 acttgggtac aaaacttgtg cttctgggca ttgtctgtct ttcaagtgca aagcagatgt
     4321 agctgatacc ctgcttgtca gaacccagtg ggttcctgca atgccccaaa tactgaaata
     4381 agaaccaaat tctggaggag ccctcctcag ggaaaagagg gactctcaaa caaacgtcag
     4441 tgacttacaa gggagacctc ttgtttaaag acttatggat caacttctgt tcctctgttt
     4501 aagagaaatt tctattgcaa aatgggtatc gttttaaatg agcagaacag attaacaaat
     4561 ggagcaattt gtttctgtta tcccaataga aaaggagatg atggggacag tgtgacaagt
     4621 caatcaagtt gcttttcccg tacacctctt ttggacgttt aatttactac tactacaagc
     4681 tactctgtct cttcccattg ctttaattct agggtttctg agtgaccaaa atgggaagga
     4741 aaaaaaaaac ctcatctcac agaggagaac tgcatgcaat aaaagattca aaagttggaa
     4801 agtagcttca aactgctgct ggctcccagc tgctgttaca gggtgggaag gtgttaggaa
     4861 atgttgtttg ttagaagctc cttttactgt agaatttaat agtgaatcct tccccaatgc
     4921 ctattcagtt gccttttggc cgttaagctc taagttcttt ggtaaagagt taatataatt
     4981 aaacattttt actgttttct attttggagt aacttggtgc tgatcttcct tccctttccc
     5041 cagctgccca acaagccacc ctttaaacac caaagacaac tggttttaaa tgcttgaaaa
     5101 aaattttatt gaggagatgg atcttgatta aaatcttttc atcatcattt tcattctgct
     5161 cttttctttc taatttcccc agatgtagga aaattttggg acctgaatga gaaatgttct
     5221 tggtgcataa cagtaactag agctccttat ttgacatttt actatggatg tgatcgaaaa
     5281 gccaagattt tcgctccatg gctgaaatta tcaggtctta atttacagac ttgtaaattt
     5341 gcaggcttag agcaactaga actatcccct gaaataggtg tgtaggtcag agatactacc
     5401 tctttgtcct cagtggtggt atatctatct ccttaccttt ctgtacccaa aatggtgcat
     5461 gactggtatt tgagaaacat ggtcacattt ggaaagcttt tgtttttaaa acattggcgg
     5521 tttttcctgc ccgtggttga ctcctgaccc atagggattg gtgcgaagca ctttggagag
     5581 tgtttattgt atgatgtgaa atgttctaaa tcaaagaaag atgataaaag ccaacatttg
     5641 gcagtcccta gtgtgaaact gtgaaaagta cctgtgttta aatgcataat ctcctgcccg
     5701 ggtaatgcca ggtagccagg agttgggtca aagaagggag tcgtcatcct gatggaaaaa
     5761 ataaggtcct gggtattatg aggtaggaga ataaaaaggt ttctggggag taagtatgac
     5821 aattactagt ccccatggag gtctgatctg gtcaggtggc aagggttggg tggggtatga
     5881 tgtaagctca gtttatgtgt ggagatgccc attgggtttg gatatatagg tttgagtatt
     5941 gagatgttta aaggacatct aacccatagg taaaaactac ttttgcttga cactgactta
     6001 acatttcaaa agtgttattt ttggtcccaa gatctaatgc tgtctcagaa catgtgccct
     6061 gttgtggctg caagtggcac tcaagttgac tagagcacag tgacctgggt tgtaccccaa
     6121 gtgcactaac tcctcaatgc ccttttaagt ctaacttatt tgggaaacac ataacaaagg
     6181 gtaccataaa ccgaatccaa attacgtgtt gggccaaaaa tgttgccaga actgaggcac
     6241 tttgtgatgg agttgcaaga gatagcttga gatcggctca gtctgattta ttctagttaa
     6301 gccaaagctt aattatttga ggaggaaagt cttttaggct tgaagcagca aaatactgtt
     6361 tatatatgtg ttaccttcct cgttggaaga ttctacgatt gctctgttct ggtaggtggt
     6421 gaaagatatg ccaaagtttt aggcttgttt ttctgatctt atttttttaa tccagtggtg
     6481 ccaaaaagta tttagggtat gtttaaggta tatttaaaac atcactctga atgagtttcc
     6541 aacagtctgg ggctgtataa aagtcacact cttgtacttg caggtgatgg tgtcacaggg
     6601 ccagggcctc cagagctcta cggctttctc tggttttcta tagggccaag ggcacaggac
     6661 gcagcattcc agaccacact gactgctttt gctgagtttc tacctcactt gtggcaagtc
     6721 atctacctca tgttagcatc tgacaggtct caaaaagaat agtattaatc cagtgggcag
     6781 tgggtgggag tgggaagcta cctctaaaag aaatgttacc ataaatctac aactgatttc
     6841 tcccaggaga aatgttctac ttaccttgaa agagaaatgt caggcaaatg tagtcataaa
     6901 tccaagtagt tcttagcaat ctggcttttc cccctcaaaa agaattatgc ataccattac
     6961 tgttgttcag tattatgaaa ctactggtta atctgaccta ttggaggact taactacaaa
     7021 taaagacctt taggtctctt tctcttcaag agaaaaatat tttatctgta tttcgaatgt
     7081 ctgcaaataa taccctttag gagcaattct aaaggtctag atcttggaaa catcaaatat
     7141 ttgaagggaa tgagaatcct cttgacttca tgattgtatt tgtagctctc tggacccggc
     7201 aggacgttca tcaccatgat gttgcaacaa tcactgtttt gactgagcag tgactgttga
     7261 agtgtgtatt gctcttttgt tttgttttta tttttttcta ccaaaggtaa catgttgacg
     7321 ggtttttttt ttccttgttt attaaatact tgataaagtt gagaagcaca ttaccaggat
     7381 atactgtatt gaccctccca cctctcttct gccctaattt ttggttgttt ggaagagagg
     7441 agggtcctaa gaccactgtc attatcatga ggggcttata aatgtcatgg tgaagaaata
     7501 cctcaatgat gtctattttt aaaactgatc ttacgggtta acaagccagc ctggtgggat
     7561 attttccatc atcatttaac caataatggt tctaaaagtt ttgtgtatcc acatggtctt
     7621 agacctcctt ttaatgattg tattaactta caagctcagg tagtattttt cttaagactc
     7681 tatctcagag cacactgact gaatgttgat gtgtgtagca aagtgtttac tttctttaaa
     7741 taaccagctc tgtaactctg taagttcttt gtgtttctag cagtctgtgt agttgtcttt
     7801 cttcagagga agatttttca catttctggg gttttcttgc ttttagggtg gtaagattcc
     7861 tttctttttt tcccttttct cctaaaatcg atgcaggtaa gggtgggtgg gtaaggggtg
     7921 ttgtttttta actagcatgt tagttatact tgggtgggtg ggagggttgt taaactgaat
     7981 cttgcttaat actgtccatt agctctcatg ccgggtcagc aaaatgcttt aatttttaac
     8041 tgcagaacat gtgactcggt tgagtctttt tgtctttttt ttttttaaat aaacaattgg
     8101 gggaattaat gtgggcaagt tgccttatgt tagaatgact aagttaaacc tcttaatttg
     8161 tatttgtcca aggcagacat gatataagga atatgcacta ccgtagtaac tcccctggcc
     8221 gcagaaacca cactgcaagc ctgtccgggg tggggtgctg actgccattt gccactttta
     8281 aatgggcact gccgtggtaa tgtgaatccc atcaatgcag tagatgggtg gggaaatgaa
     8341 gatttccccc caaaccttta ggcaaaagtg agtttttgtt tggttgattt tgggtttttt
     8401 tcccccctcc tcttttggct ttcacatttt aaatcttaaa tgttactatt gcaggccgca
     8461 ggcatgacag gcaatgagca ggtgaagaac caatggaaaa gtgttcaaaa actcctgtgt
     8521 tagctaacag gcttctgaat gtatcactgt ggtccacaga gaaggctgga ggaggtagca
     8581 aggagatgct gtatcagcta ctacagcctt aaaacaaagt tggtgtctct ttggacttta
     8641 aaattgttcc tatgcagctt attttatttt tgtttaatca aataaacgag ggtttttcca
     8701 tggc
//
