LOCUS       NM_173547               3384 bp    mRNA    linear   PRI 11-MAR-2025
DEFINITION  Homo sapiens tripartite motif containing 65 (TRIM65), transcript
            variant 1, mRNA.
ACCESSION   NM_173547
VERSION     NM_173547.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Bian,Z., Xu,C., Wang,X., Zhang,B., Xiao,Y., Liu,L., Zhao,S.,
            Huang,N., Yang,F., Zhang,Y., Xue,S., Wang,X., Pan,Q. and Sun,F.
  TITLE     TRIM65/NF2/YAP1 Signaling Coordinately Orchestrates Metabolic and
            Immune Advantages in Hepatocellular Carcinoma
  JOURNAL   Adv Sci (Weinh) 11 (35), e2402578 (2024)
   PUBMED   39005234
  REMARK    GeneRIF: TRIM65/NF2/YAP1 Signaling Coordinately Orchestrates
            Metabolic and Immune Advantages in Hepatocellular Carcinoma.
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Shen,S., Yan,R., Xie,Z., Yu,X., Liang,H., You,Q., Zhang,H., Hou,J.,
            Zhang,X., Liu,Y., Sun,J. and Guo,H.
  TITLE     Tripartite Motif-Containing Protein 65 (TRIM65) Inhibits Hepatitis
            B Virus Transcription
  JOURNAL   Viruses 16 (6), 890 (2024)
   PUBMED   38932182
  REMARK    GeneRIF: Tripartite Motif-Containing Protein 65 (TRIM65) Inhibits
            Hepatitis B Virus Transcription.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3384)
  AUTHORS   Zhou,Z.X., Ma,X.F., Xiong,W.H., Ren,Z., Jiang,M., Deng,N.H.,
            Zhou,B.B., Liu,H.T., Zhou,K., Hu,H.J., Tang,H.F., Zheng,H. and
            Jiang,Z.S.
  TITLE     TRIM65 promotes vascular smooth muscle cell phenotypic
            transformation by activating PI3K/Akt/mTOR signaling during
            atherogenesis
  JOURNAL   Atherosclerosis 390, 117430 (2024)
   PUBMED   38301602
  REMARK    GeneRIF: TRIM65 promotes vascular smooth muscle cell phenotypic
            transformation by activating PI3K/Akt/mTOR signaling during
            atherogenesis.
REFERENCE   4  (bases 1 to 3384)
  AUTHORS   Jiang,M., Wang,D., Su,N., Lou,W., Chen,Y., Yang,H., Chen,C., Xi,F.,
            Chen,Y., Deng,L. and Tang,X.
  TITLE     TRIM65 knockout inhibits the development of HCC by polarization
            tumor-associated macrophages towards M1 phenotype via JAK1/STAT1
            signaling pathway
  JOURNAL   Int Immunopharmacol 128, 111494 (2024)
   PUBMED   38218012
  REMARK    GeneRIF: TRIM65 knockout inhibits the development of HCC by
            polarization tumor-associated macrophages towards M1 phenotype via
            JAK1/STAT1 signaling pathway.
REFERENCE   5  (bases 1 to 3384)
  AUTHORS   Ma,X.F., Zhou,Y.R., Zhou,Z.X., Liu,H.T., Zhou,B.B., Deng,N.H.,
            Zhou,K., Tian,Z., Wu,Z.F., Liu,X.Y., Fu,M.G. and Jiang,Z.S.
  TITLE     TRIM65 Suppresses oxLDL-induced Endothelial Inflammation by
            Interaction with VCAM-1 in Atherogenesis
  JOURNAL   Curr Med Chem 31 (30), 4898-4911 (2024)
   PUBMED   37608612
  REMARK    GeneRIF: TRIM65 Suppresses oxLDL-induced Endothelial Inflammation
            by Interaction with VCAM-1 in Atherogenesis.
REFERENCE   6  (bases 1 to 3384)
  AUTHORS   Huang,W.Q., Ye,H.M., Li,F.F., Yi,K.H., Zhang,Y., Cai,L.L.,
            Lin,H.N., Lin,Q. and Tzeng,C.M.
  TITLE     Analysis of genetic polymorphisms associated with leukoaraiosis in
            the southern Chinese population: A case-control study
  JOURNAL   Medicine (Baltimore) 95 (35), e3857 (2016)
   PUBMED   27583843
  REMARK    GeneRIF: These results suggest that rs3744028 in TRIM65 is not
            associated with leukoaraiosis (LA) risk in the Chinese population.
            However, the association of rs3744028 (TRIM65) with LA before
            Bonferroni correction and Sidak correction is worth highlighting.
REFERENCE   7  (bases 1 to 3384)
  AUTHORS   Li,Y., Ma,C., Zhou,T., Liu,Y., Sun,L. and Yu,Z.
  TITLE     TRIM65 negatively regulates p53 through ubiquitination
  JOURNAL   Biochem Biophys Res Commun 473 (1), 278-282 (2016)
   PUBMED   27012201
  REMARK    GeneRIF: TRIM65 is a potential oncogenic protein, highly likely
            through p53 inactivation
REFERENCE   8  (bases 1 to 3384)
  AUTHORS   Kimura,T., Jain,A., Choi,S.W., Mandell,M.A., Schroder,K.,
            Johansen,T. and Deretic,V.
  TITLE     TRIM-mediated precision autophagy targets cytoplasmic regulators of
            innate immunity
  JOURNAL   J Cell Biol 210 (6), 973-989 (2015)
   PUBMED   26347139
REFERENCE   9  (bases 1 to 3384)
  AUTHORS   Li,S., Wang,L., Fu,B., Berman,M.A., Diallo,A. and Dorf,M.E.
  TITLE     TRIM65 regulates microRNA activity by ubiquitination of TNRC6
  JOURNAL   Proc Natl Acad Sci U S A 111 (19), 6970-6975 (2014)
   PUBMED   24778252
  REMARK    GeneRIF: TRIM65 relieves miRNA-driven suppression of mRNA
            expression through ubiquitination and subsequent degradation of
            TNRC6.
REFERENCE   10 (bases 1 to 3384)
  AUTHORS   Fornage,M., Debette,S., Bis,J.C., Schmidt,H., Ikram,M.A.,
            Dufouil,C., Sigurdsson,S., Lumley,T., DeStefano,A.L., Fazekas,F.,
            Vrooman,H.A., Shibata,D.K., Maillard,P., Zijdenbos,A., Smith,A.V.,
            Gudnason,H., de Boer,R., Cushman,M., Mazoyer,B., Heiss,G.,
            Vernooij,M.W., Enzinger,C., Glazer,N.L., Beiser,A., Knopman,D.S.,
            Cavalieri,M., Niessen,W.J., Harris,T.B., Petrovic,K., Lopez,O.L.,
            Au,R., Lambert,J.C., Hofman,A., Gottesman,R.F., Garcia,M.,
            Heckbert,S.R., Atwood,L.D., Catellier,D.J., Uitterlinden,A.G.,
            Yang,Q., Smith,N.L., Aspelund,T., Romero,J.R., Rice,K.,
            Taylor,K.D., Nalls,M.A., Rotter,J.I., Sharrett,R., van Duijn,C.M.,
            Amouyel,P., Wolf,P.A., Gudnason,V., van der Lugt,A., Boerwinkle,E.,
            Psaty,B.M., Seshadri,S., Tzourio,C., Breteler,M.M., Mosley,T.H.,
            Schmidt,R., Longstreth,W.T., DeCarli,C. and Launer,L.J.
  TITLE     Genome-wide association studies of cerebral white matter lesion
            burden: the CHARGE consortium
  JOURNAL   Ann Neurol 69 (6), 928-939 (2011)
   PUBMED   21681796
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC021259.2, AI242308.1 and AC087289.9.
            
            On Nov 23, 2018 this sequence version replaced NM_173547.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC021259.2, SRR14038193.212484.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000269383.8/ ENSP00000269383.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-678               BC021259.2         21-698
            679-750             AI242308.1         348-419
            751-1539            BC021259.2         771-1559
            1540-3272           AC087289.9         62580-64312
            3273-3384           BC021259.2         3294-3405
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q25.1"
     gene            1..3384
                     /gene="TRIM65"
                     /gene_synonym="4732463G12Rik"
                     /note="tripartite motif containing 65"
                     /db_xref="GeneID:201292"
                     /db_xref="HGNC:HGNC:27316"
                     /db_xref="MIM:619408"
     exon            1..428
                     /gene="TRIM65"
                     /gene_synonym="4732463G12Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             15..1568
                     /gene="TRIM65"
                     /gene_synonym="4732463G12Rik"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1;
                     tripartite motif-containing protein 65; E3
                     ubiquitin-protein ligase TRIM65"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase TRIM65 isoform 1"
                     /protein_id="NP_775818.2"
                     /db_xref="CCDS:CCDS11732.1"
                     /db_xref="GeneID:201292"
                     /db_xref="HGNC:HGNC:27316"
                     /db_xref="MIM:619408"
                     /translation="MAAQLLEEKLTCAICLGLYQDPVTLPCGHNFCGACIRDWWDRCG
                     KACPECREPFPDGAELRRNVALSGVLEVVRAGPARDPGPDPGPGPDPAARCPRHGRPL
                     ELFCRTEGRCVCSVCTVRECRLHERALLDAERLKREAQLRASLEVTQQQATQAEGQLL
                     ELRKQSSQIQNSACILASWVSGKFSSLLQALEIQHTTALRSIEVAKTQALAQARDEEQ
                     RLRVHLEAVARHGCRIRELLEQVDEQTFLQESQLLQPPGPLGPLTPLQWDEDQQLGDL
                     KQLLSRLCGLLLEEGSHPGAPAKPVDLAPVEAPGPLAPVPSTVCPLRRKLWQNYRNLT
                     FDPVSANRHFYLSRQDQQVKHCRQSRGPGGPGSFELWQVQCAQSFQAGHHYWEVRASD
                     HSVTLGVSYPQLPRCRLGPHTDNIGRGPCSWGLCVQEDSLQAWHNGEAQRLPGVSGRL
                     LGMDLDLASGCLTFYSLEPQTQPLYTFHALFNQPLTPVFWLLEGRTLTLCHQPGAVFP
                     LGPQEEVLS"
     exon            429..524
                     /gene="TRIM65"
                     /gene_synonym="4732463G12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            525..758
                     /gene="TRIM65"
                     /gene_synonym="4732463G12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            759..933
                     /gene="TRIM65"
                     /gene_synonym="4732463G12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            934..999
                     /gene="TRIM65"
                     /gene_synonym="4732463G12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..3384
                     /gene="TRIM65"
                     /gene_synonym="4732463G12Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3365..3370
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRIM65"
                     /gene_synonym="4732463G12Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3384
                     /gene="TRIM65"
                     /gene_synonym="4732463G12Rik"
                     /note="major polyA site"
ORIGIN      
        1 gccgccgccg ggccatggcc gcgcagctgc tggaggagaa gctgacctgc gccatctgcc
       61 tggggctcta ccaggaccca gtgacgctgc cctgcggcca caacttctgc ggggcctgca
      121 tccgggactg gtgggaccgc tgcggaaagg cgtgccccga gtgccgggag ccctttcccg
      181 acggcgccga gctgcgccgc aacgtggccc tcagcggcgt gctggaggtg gtgcgcgccg
      241 ggcccgcccg ggatcccggc cccgatcccg gccccggccc cgaccctgcc gcgcgctgcc
      301 cccgccacgg gcggccgctg gagctcttct gccggaccga gggccgctgt gtgtgcagcg
      361 tgtgcaccgt gcgcgagtgt cgcctccacg agcgggcgct gctggatgcc gagcgcctca
      421 agcgcgaggc ccagctgaga gccagcctgg aggttaccca gcagcaggcc acccaggccg
      481 aaggccagct actagagctg cgcaagcaaa gcagccagat ccagaactcg gcctgcatct
      541 tggcctcctg ggtctccggc aagttcagca gcctgctaca ggccctggaa atacagcaca
      601 cgacagcact gaggagcatc gaggtggcca agacgcaggc gctggcacag gctcgagacg
      661 aggagcagcg gctgcgggtc catttggagg ctgtggctcg ccatggctgc aggatccggg
      721 agctcctgga gcaggtggat gagcagacct tcctgcagga atcgcagctc ctccagcccc
      781 cagggcctct tgggccactg acccctctgc agtgggatga agaccaacag ctgggtgacc
      841 tgaagcagtt gctaagccgg ctgtgtggcc tcctcttgga agaggggagc caccctgggg
      901 caccagccaa gcctgtggac ttagcccccg tggaggcccc aggtcccctg gcaccggtcc
      961 caagcacagt ttgtccactg aggaggaaac tctggcagaa ttatcgcaat ctgacctttg
     1021 atccagtcag cgccaaccgt cacttctatc tgtcgcgcca ggaccagcag gtgaagcact
     1081 gtcgtcagtc ccggggccca ggcgggcccg gcagctttga gctctggcag gtgcaatgtg
     1141 cccagagctt ccaggccggg caccactact gggaggtgcg cgcgtcagac cactcggtga
     1201 cactgggcgt ctcctacccg caactgccac ggtgcaggct ggggccccac acagacaaca
     1261 ttggccgggg accctgctcc tgggggctct gcgtccagga ggacagcctc caggcctggc
     1321 acaacgggga agcccagcgc ctcccagggg tgtcagggcg gctcctgggc atggatttgg
     1381 acctggcctc aggctgcctc accttctaca gcctggagcc ccagacccag cccctgtaca
     1441 ccttccatgc cctcttcaac cagcccctca cccccgtctt ctggctcctc gagggtagga
     1501 ccctgaccct gtgccatcag ccaggggctg tgttccctct ggggccccag gaagaggtgc
     1561 tcagctgaag aaggcatggg atggagccct ggcatagctg ccaccatgcc tatgtgccca
     1621 agagctgccc agcttcagct tggggactgg aggaccagct gttggcctct ctgttaactc
     1681 agaaagagat gggaggttgg gggaggtgag cataaacgca gagttcactg ttgcagcctt
     1741 tttgaagggg acacagtcta ggagggggat aaatgggatg cccttgcccc agagagaacc
     1801 cagttctagg tactgtctgg gcctgggagg cgagagcagt gcccagggga cttctgggct
     1861 tacaggacag cgtgtgtgac aaaattcaga tctacctgaa cttgcctctg gagatgataa
     1921 gggccaaagg agcagtcagg gaggggcggt gagccagagt agtcccaggg ggagacagat
     1981 tcctccctcc tccccgcctg cagctctctt taattttttg taacatttgg agagacgtcc
     2041 gtcctgtctt gtagtctttt tattttgtgc atccttataa ttgtattcta caaacaattt
     2101 tgttttctgc atttaaacat ttttgtgttt ttaggagatg gtcttgctct gtcactcagg
     2161 ctggagtaca gtggcacaat catggctcac tgcagcctcg agctcctgag ctcaaaggat
     2221 cctcccacct cagcctccag agtagctcag attacagtgt tttctgaagt ttcctgactt
     2281 aacataagca tttttcacat gattgaaaat tttcactgat tgctgtagtc attctaatga
     2341 ctatcaagtt tatttatcca ttattggaca tttaaatttc tttcttttta gctatacaaa
     2401 cattgatgtg ttttttttga gacaggatct ccatctgtca cccaggctgg tgtgcagggg
     2461 tgcggtcatg ggctcatggg attctcctac ctcagcctcc tgagtagctg ggattacagg
     2521 tgtatgccac cacgcctggc ttatgatgaa tatttctgag tgggatgttt ggccacattt
     2581 atggtcatgt atgtaggctg ttacctcaag tgagtcactc agggaacaat gagcacttga
     2641 agattttttt atacaaaagg ccacagtgag gccaccttga gtcaagccga ctaaggcccc
     2701 tcaaccctgt cactaagcag cacgtgacac tggcaggacc ttcatctcca gcatcccacc
     2761 cctgggtgtg ggactttggg gcagccgtgt gtgcaggtgt cggcacaggc tagctcctcc
     2821 tggtttggtg tggtgtttgc cattgcagag caagctgcca cgaagacccc tgggcatgat
     2881 tttgcttgta tttccggaag tggggttgct gggtcatagg gcaggtgtaa ttttttttac
     2941 ttgaaatgtt ccacttcttg ttctgggagg tggttccaag ggtgtctgct tttggccagg
     3001 cgcagtggct cacgcttgta atcccagcac tttgggaggc cgaggcaggc ggatcacaag
     3061 gtcaggagtt cgagaccagc ctggccaaca aggcaaaacc ccgtctctac taaaaataca
     3121 aaaattagcc gggcatggtg gtgtgcgcct gtaatcccag ctactgggga ggctgaggca
     3181 gaagaatcgc ttgaacccag gaggtgaggt tgcagtgagc tgagattgca ccactggact
     3241 acagcctggg tgacagagcg agactctgtc tcaaaaaaaa aaaaaaaaag ggagggctgt
     3301 ctgctttttg taacttgaaa aactggacat ctatgttata tgtactcatg gataaatatt
     3361 tcacaataaa actttttaaa agga
//
