LOCUS       NM_187841               1930 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens tripartite motif containing 54 (TRIM54), transcript
            variant 2, mRNA.
ACCESSION   NM_187841
VERSION     NM_187841.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Liu,M.R., Shi,C., Song,Q.Y., Kang,M.J., Jiang,X., Liu,H. and
            Pei,D.S.
  TITLE     Sorafenib induces ferroptosis by promoting TRIM54-mediated FSP1
            ubiquitination and degradation in hepatocellular carcinoma
  JOURNAL   Hepatol Commun 7 (10), e0246 (2023)
   PUBMED   37695069
  REMARK    GeneRIF: Sorafenib induces ferroptosis by promoting TRIM54-mediated
            FSP1 ubiquitination and degradation in hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Kim,D.K., Weller,B., Lin,C.W., Sheykhkarimli,D., Knapp,J.J.,
            Dugied,G., Zanzoni,A., Pons,C., Tofaute,M.J., Maseko,S.B.,
            Spirohn,K., Laval,F., Lambourne,L., Kishore,N., Rayhan,A.,
            Sauer,M., Young,V., Halder,H., la Rosa,N.M., Pogoutse,O.,
            Strobel,A., Schwehn,P., Li,R., Rothballer,S.T., Altmann,M.,
            Cassonnet,P., Cote,A.G., Vergara,L.E., Hazelwood,I., Liu,B.B.,
            Nguyen,M., Pandiarajan,R., Dohai,B., Coloma,P.A.R., Poirson,J.,
            Giuliana,P., Willems,L., Taipale,M., Jacob,Y., Hao,T., Hill,D.E.,
            Brun,C., Twizere,J.C., Krappmann,D., Heinig,M., Falter,C., Aloy,P.,
            Demeret,C., Vidal,M., Calderwood,M.A., Roth,F.P. and
            Falter-Braun,P.
  TITLE     A proteome-scale map of the SARS-CoV-2-human contactome
  JOURNAL   Nat Biotechnol 41 (1), 140-149 (2023)
   PUBMED   36217029
REFERENCE   3  (bases 1 to 1930)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1930)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1930)
  AUTHORS   Nowak,M., Suenkel,B., Porras,P., Migotti,R., Schmidt,F., Kny,M.,
            Zhu,X., Wanker,E.E., Dittmar,G., Fielitz,J. and Sommer,T.
  TITLE     DCAF8, a novel MuRF1 interaction partner, promotes muscle atrophy
  JOURNAL   J Cell Sci 132 (17) (2019)
   PUBMED   31391242
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1930)
  AUTHORS   Chen,S., Fragoza,R., Klei,L., Liu,Y., Wang,J., Roeder,K., Devlin,B.
            and Yu,H.
  TITLE     An interactome perturbation framework prioritizes damaging missense
            mutations for developmental disorders
  JOURNAL   Nat Genet 50 (7), 1032-1040 (2018)
   PUBMED   29892012
REFERENCE   7  (bases 1 to 1930)
  AUTHORS   Olive,M., Abdul-Hussein,S., Oldfors,A., Gonzalez-Costello,J., van
            der Ven,P.F., Furst,D.O., Gonzalez,L., Moreno,D.,
            Torrejon-Escribano,B., Alio,J., Pou,A., Ferrer,I. and Tajsharghi,H.
  TITLE     New cardiac and skeletal protein aggregate myopathy associated with
            combined MuRF1 and MuRF3 mutations
  JOURNAL   Hum Mol Genet 24 (13), 3638-3650 (2015)
   PUBMED   25801283
  REMARK    GeneRIF: A novel protein aggregate myopathies and cardiomyopathy
            resulting from combined homozygous MuRF1 null mutation and
            heterozygous MuRF3 missense mutation.
            Erratum:[Hum Mol Genet. 2015 Nov 1;24(21):6264. doi:
            10.1093/hmg/ddv311.. PMID: 26345447]
REFERENCE   8  (bases 1 to 1930)
  AUTHORS   Sahni,N., Yi,S., Taipale,M., Fuxman Bass,J.I.,
            Coulombe-Huntington,J., Yang,F., Peng,J., Weile,J., Karras,G.I.,
            Wang,Y., Kovacs,I.A., Kamburov,A., Krykbaeva,I., Lam,M.H.,
            Tucker,G., Khurana,V., Sharma,A., Liu,Y.Y., Yachie,N., Zhong,Q.,
            Shen,Y., Palagi,A., San-Miguel,A., Fan,C., Balcha,D., Dricot,A.,
            Jordan,D.M., Walsh,J.M., Shah,A.A., Yang,X., Stoyanova,A.K.,
            Leighton,A., Calderwood,M.A., Jacob,Y., Cusick,M.E.,
            Salehi-Ashtiani,K., Whitesell,L.J., Sunyaev,S., Berger,B.,
            Barabasi,A.L., Charloteaux,B., Hill,D.E., Hao,T., Roth,F.P.,
            Xia,Y., Walhout,A.J.M., Lindquist,S. and Vidal,M.
  TITLE     Widespread macromolecular interaction perturbations in human
            genetic disorders
  JOURNAL   Cell 161 (3), 647-660 (2015)
   PUBMED   25910212
REFERENCE   9  (bases 1 to 1930)
  AUTHORS   Centner,T., Yano,J., Kimura,E., McElhinny,A.S., Pelin,K.,
            Witt,C.C., Bang,M.L., Trombitas,K., Granzier,H., Gregorio,C.C.,
            Sorimachi,H. and Labeit,S.
  TITLE     Identification of muscle specific ring finger proteins as potential
            regulators of the titin kinase domain
  JOURNAL   J Mol Biol 306 (4), 717-726 (2001)
   PUBMED   11243782
REFERENCE   10 (bases 1 to 1930)
  AUTHORS   Spencer,J.A., Eliazer,S., Ilaria,R.L. Jr., Richardson,J.A. and
            Olson,E.N.
  TITLE     Regulation of microtubule dynamics and myogenic differentiation by
            MURF, a striated muscle RING-finger protein
  JOURNAL   J Cell Biol 150 (4), 771-784 (2000)
   PUBMED   10953002
  REMARK    GeneRIF: Functional analysis of the mouse counterpart.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA897004.1 and BC141807.1.
            
            On Jun 2, 2019 this sequence version replaced NM_187841.2.
            
            Summary: The protein encoded by this gene contains a RING finger
            motif and is highly similar to the ring finger proteins RNF28/MURF1
            and RNF29/MURF2. In vitro studies demonstrated that this protein,
            RNF28, and RNF29 form heterodimers, which may be important for the
            regulation of titin kinase and microtubule-dependent signal
            pathways in striated muscles. Alternatively spliced transcript
            variants encoding distinct isoforms have been reported. [provided
            by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) lacks an in-frame segment
            within the coding region. The resulting shorter isoform (2) lacks
            an internal region, as compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC141807.1, ERR279839.2774.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2145743
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000380075.7/ ENSP00000369415.3
            RefSeq Select criteria :: based on manual assertion, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-228               DA897004.1         1-228
            229-1930            BC141807.1         1-1702
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p23.3"
     gene            1..1930
                     /gene="TRIM54"
                     /gene_synonym="MURF; MURF-3; muRF3; RNF30"
                     /note="tripartite motif containing 54"
                     /db_xref="GeneID:57159"
                     /db_xref="HGNC:HGNC:16008"
                     /db_xref="MIM:606474"
     exon            1..471
                     /gene="TRIM54"
                     /gene_synonym="MURF; MURF-3; muRF3; RNF30"
                     /inference="alignment:Splign:2.1.0"
     CDS             304..1380
                     /gene="TRIM54"
                     /gene_synonym="MURF; MURF-3; muRF3; RNF30"
                     /note="isoform 2 is encoded by transcript variant 2; ring
                     finger protein 30; tripartite motif-containing protein 54;
                     muscle-specific RING finger protein 3"
                     /codon_start=1
                     /product="tripartite motif-containing protein 54 isoform
                     2"
                     /protein_id="NP_912730.2"
                     /db_xref="CCDS:CCDS1746.2"
                     /db_xref="GeneID:57159"
                     /db_xref="HGNC:HGNC:16008"
                     /db_xref="MIM:606474"
                     /translation="MNFTVGFKPLLGDAHSMDNLEKQLICPICLEMFSKPVVILPCQH
                     NLCRKCANDVFQASNPLWQSRGSTTVSSGGRFRCPSCRHEVVLDRHGVYGLQRNLLVE
                     NIIDIYKQESSRPLHSKAEQHLMCEEHEEEKINIYCLSCEVPTCSLCKVFGAHKDCEV
                     APLPTIYKRQKSELSDGIAMLVAGNDRVQAVITQMEEVCQTIEDNSRRQKQLLNQRFE
                     SLCAVLEERKGELLQALAREQEEKLQRVRGLIRQYGDHLEASSKLVESAIQSMEEPQM
                     ALYLQQAKELINKVGAMSKVELAGRPEPGYESMEQFTVRVEHVAEMLRTIDFQPGASG
                     EEEEVAPDGEEGSAGPEEERPDGP"
     exon            472..644
                     /gene="TRIM54"
                     /gene_synonym="MURF; MURF-3; muRF3; RNF30"
                     /inference="alignment:Splign:2.1.0"
     exon            645..816
                     /gene="TRIM54"
                     /gene_synonym="MURF; MURF-3; muRF3; RNF30"
                     /inference="alignment:Splign:2.1.0"
     exon            817..912
                     /gene="TRIM54"
                     /gene_synonym="MURF; MURF-3; muRF3; RNF30"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1146
                     /gene="TRIM54"
                     /gene_synonym="MURF; MURF-3; muRF3; RNF30"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1169
                     /gene="TRIM54"
                     /gene_synonym="MURF; MURF-3; muRF3; RNF30"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1294
                     /gene="TRIM54"
                     /gene_synonym="MURF; MURF-3; muRF3; RNF30"
                     /inference="alignment:Splign:2.1.0"
     exon            1295..1381
                     /gene="TRIM54"
                     /gene_synonym="MURF; MURF-3; muRF3; RNF30"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1930
                     /gene="TRIM54"
                     /gene_synonym="MURF; MURF-3; muRF3; RNF30"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1912..1917
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRIM54"
                     /gene_synonym="MURF; MURF-3; muRF3; RNF30"
                     /note="hexamer: ATTAAA"
     polyA_site      1930
                     /gene="TRIM54"
                     /gene_synonym="MURF; MURF-3; muRF3; RNF30"
                     /note="major polyA site"
ORIGIN      
        1 aaacagtgct acccacagag tgaacaagag agagtcattt gggaaacaaa aggagaattt
       61 tacagagaga gagggatagc taaaactacg tgagcctggc gagggtgcag agcagaaagt
      121 agagactgtc cgaagactgc tatctgggac gagacaagtt gttaaaggga caggagagaa
      181 agcagagcta tttcaagagt gagccacaga agggaatcca gaggccatct aagcgaggaa
      241 gggtctacag gcagtgagtg aaggccagga gcagggccca ggccaggcac gaccaccgag
      301 gggatgaact tcacagtggg tttcaagccg ctgctagggg atgcacacag catggacaac
      361 ctggagaagc agctcatctg ccccatctgc ctggagatgt tctccaaacc agtggtgatc
      421 ctgccctgcc aacacaacct gtgccgcaaa tgtgccaacg acgtcttcca ggcctcgaat
      481 cctctatggc agtcccgggg ctccaccact gtgtcttcag gaggccgttt ccgctgccca
      541 tcgtgcaggc atgaggttgt cctggacaga cacggtgtct acggcctgca gcgaaacctg
      601 ctagtggaga acattatcga catttacaag caggagtcat ccaggccgct gcactccaag
      661 gctgagcagc acctcatgtg cgaggagcat gaagaagaga agatcaatat ttactgcctg
      721 agctgtgagg tgcccacctg ctctctctgc aaggtcttcg gtgcccacaa ggactgtgag
      781 gtggccccac tgcccaccat ttacaaacgc cagaagagtg agctcagcga tggcatcgcg
      841 atgctggtgg caggcaatga ccgcgtgcaa gcagtgatca cacagatgga ggaggtgtgc
      901 cagactatcg aggacaatag ccggaggcag aagcagttgt taaaccagag gtttgagagc
      961 ctgtgcgcag tgctggagga gcgcaagggt gagctgctgc aggcgctggc ccgggagcaa
     1021 gaggagaagc tgcagcgcgt ccgcggcctc atccgtcagt atggcgacca cctggaggcc
     1081 tcctctaagc tggtggagtc tgccatccag tccatggaag agccacaaat ggcgctgtat
     1141 ctccagcagg ccaaggagct gatcaataag gtcggggcca tgtcgaaggt ggagctggca
     1201 gggcggccgg agccaggcta tgagagcatg gagcaattca ccgtaagggt ggagcacgtg
     1261 gccgaaatgc tgcggaccat cgacttccag ccaggcgctt ccggggagga agaggaggtg
     1321 gccccagacg gagaggaggg cagcgcgggg ccggaggaag agcggccgga tgggccttaa
     1381 ggcctgcgcc gacccgaccc tgctcgagag cccgcgctag agtcggggag gatctgcgca
     1441 gagaccgcag catcacccaa atcggcgccg gccccgggag gatctcaata aagaactcga
     1501 gcgtcccaga cccgtatctc ctttcgctgc ccaaccccgc agcctgggct tcgaaggcga
     1561 cccgcccacc atcctgccct tcccagaacc tgagaccgtc tggggggcgg aagccaaatg
     1621 aacccctatt gggcacctct gtgatgccag gagcgaactg gtgagcccag cgccctggga
     1681 agagggccga gggcggggcg gtggtgccgg gacctctgag gtcctgggga tttggggacc
     1741 cttggggtcc acatgcacct ggctgacctg gctgaaagcc gctgtctcgg agccccccac
     1801 agcattttgt tcccctcccg ctggcccggg ggccccacct tcccacgggt tcccacgctg
     1861 ctgtgactgc cctgcctcta cgacaaaagc caacgggtct tcagtacttt tattaaaaaa
     1921 tagtcacgca
//
