LOCUS       NM_178125               2050 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens tripartite motif containing 50 (TRIM50), transcript
            variant 1, mRNA.
ACCESSION   NM_178125
VERSION     NM_178125.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Hu,J., Huang,R., Liang,C., Wang,Y., Wang,M., Chen,Y., Wu,C.,
            Zhang,J., Liu,Z., Zhao,Q., Liu,Z., Wang,F. and Yuan,S.
  TITLE     TRIM50 Inhibits Gastric Cancer Progression by Regulating the
            Ubiquitination and Nuclear Translocation of JUP
  JOURNAL   Mol Cancer Res 21 (10), 1107-1119 (2023)
   PUBMED   37409971
  REMARK    GeneRIF: TRIM50 Inhibits Gastric Cancer Progression by Regulating
            the Ubiquitination and Nuclear Translocation of JUP.
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Kim,D.K., Weller,B., Lin,C.W., Sheykhkarimli,D., Knapp,J.J.,
            Dugied,G., Zanzoni,A., Pons,C., Tofaute,M.J., Maseko,S.B.,
            Spirohn,K., Laval,F., Lambourne,L., Kishore,N., Rayhan,A.,
            Sauer,M., Young,V., Halder,H., la Rosa,N.M., Pogoutse,O.,
            Strobel,A., Schwehn,P., Li,R., Rothballer,S.T., Altmann,M.,
            Cassonnet,P., Cote,A.G., Vergara,L.E., Hazelwood,I., Liu,B.B.,
            Nguyen,M., Pandiarajan,R., Dohai,B., Coloma,P.A.R., Poirson,J.,
            Giuliana,P., Willems,L., Taipale,M., Jacob,Y., Hao,T., Hill,D.E.,
            Brun,C., Twizere,J.C., Krappmann,D., Heinig,M., Falter,C., Aloy,P.,
            Demeret,C., Vidal,M., Calderwood,M.A., Roth,F.P. and
            Falter-Braun,P.
  TITLE     A proteome-scale map of the SARS-CoV-2-human contactome
  JOURNAL   Nat Biotechnol 41 (1), 140-149 (2023)
   PUBMED   36217029
REFERENCE   3  (bases 1 to 2050)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2050)
  AUTHORS   Fusco,C., Mandriani,B., Di Rienzo,M., Micale,L., Malerba,N.,
            Cocciadiferro,D., Sjottem,E., Augello,B., Squeo,G.M., Pellico,M.T.,
            Jain,A., Johansen,T., Fimia,G.M. and Merla,G.
  TITLE     TRIM50 regulates Beclin 1 proautophagic activity
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1865 (6), 908-919 (2018)
   PUBMED   29604308
  REMARK    GeneRIF: TRIM50, via the RING domain, ubiquitinates Beclin 1 in a
            K63-dependent manner enhancing its binding with ULK1 and autophagy
            activity.
REFERENCE   5  (bases 1 to 2050)
  AUTHORS   Ma,X., Ma,X., Qiu,Y., Zhu,L., Lin,Y., You,Y., Ma,D., Qin,Z.,
            Sun,C., Zhao,Y., Sun,Y. and Han,L.
  TITLE     TRIM50 suppressed hepatocarcinoma progression through directly
            targeting SNAIL for ubiquitous degradation
  JOURNAL   Cell Death Dis 9 (6), 608 (2018)
   PUBMED   29789583
  REMARK    GeneRIF: TRIM50 acted as a tumor suppressor in hepatocarcinoma
            cells by directly targeting SNAIL and reversing
            epithelial-to-mesenchymal transition.
            Erratum:[Cell Death Dis. 2018 Oct 9;9(10):1029. doi:
            10.1038/s41419-018-0835-z.. PMID: 30301954]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2050)
  AUTHORS   Kim,Y.J., Ahn,K., Gim,J.A., Oh,M.H., Han,K. and Kim,H.S.
  TITLE     Gene structure variation in segmental duplication block C of human
            chromosome 7q 11.23 during primate evolution
  JOURNAL   Gene 573 (2), 285-295 (2015)
   PUBMED   26196062
REFERENCE   7  (bases 1 to 2050)
  AUTHORS   Fusco,C., Micale,L., Augello,B., Mandriani,B., Pellico,M.T., De
            Nittis,P., Calcagni,A., Monti,M., Cozzolino,F., Pucci,P. and
            Merla,G.
  TITLE     HDAC6 mediates the acetylation of TRIM50
  JOURNAL   Cell Signal 26 (2), 363-369 (2014)
   PUBMED   24308962
  REMARK    GeneRIF: TRIM50 K372 residue competes for post-translational
            protein modifications such as acetylation and ubiquitination.
REFERENCE   8  (bases 1 to 2050)
  AUTHORS   Zurek,B., Schoultz,I., Neerincx,A., Napolitano,L.M., Birkner,K.,
            Bennek,E., Sellge,G., Lerm,M., Meroni,G., Soderholm,J.D. and
            Kufer,T.A.
  TITLE     TRIM27 negatively regulates NOD2 by ubiquitination and proteasomal
            degradation
  JOURNAL   PLoS One 7 (7), e41255 (2012)
   PUBMED   22829933
REFERENCE   9  (bases 1 to 2050)
  AUTHORS   Fusco,C., Micale,L., Egorov,M., Monti,M., D'Addetta,E.V.,
            Augello,B., Cozzolino,F., Calcagni,A., Fontana,A., Polishchuk,R.S.,
            Didelot,G., Reymond,A., Pucci,P. and Merla,G.
  TITLE     The E3-ubiquitin ligase TRIM50 interacts with HDAC6 and p62, and
            promotes the sequestration and clearance of ubiquitinated proteins
            into the aggresome
  JOURNAL   PLoS One 7 (7), e40440 (2012)
   PUBMED   22792322
  REMARK    GeneRIF: When the proteasome activity is impaired, TRIM50 fails to
            drive its substrates to the proteasome-mediated degradation.
REFERENCE   10 (bases 1 to 2050)
  AUTHORS   Micale,L., Fusco,C., Augello,B., Napolitano,L.M., Dermitzakis,E.T.,
            Meroni,G., Merla,G. and Reymond,A.
  TITLE     Williams-Beuren syndrome TRIM50 encodes an E3 ubiquitin ligase
  JOURNAL   Eur J Hum Genet 16 (9), 1038-1049 (2008)
   PUBMED   18398435
  REMARK    GeneRIF: Encodes an E3 ubiquitin ligase; putatively involved in
            phenotype determination of Williams-Beuren syndrome.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK057366.1, HY014417.1, BC112154.1, AY081948.1 and AI208986.1.
            
            On Jul 26, 2013 this sequence version replaced NM_178125.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (a). Variants 1 and 2
            encode the same isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY081948.1, BC112152.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1968968,
                                           SAMEA1970526 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000333149.7/ ENSP00000327994.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 AK057366.1         1-9
            10-158              HY014417.1         2-150
            159-1736            BC112154.1         1-1578
            1737-2047           AY081948.1         1737-2047
            2048-2050           AI208986.1         2-4                 c
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q11.23"
     gene            1..2050
                     /gene="TRIM50"
                     /gene_synonym="TRIM50A"
                     /note="tripartite motif containing 50"
                     /db_xref="GeneID:135892"
                     /db_xref="HGNC:HGNC:19017"
                     /db_xref="MIM:612548"
     exon            1..183
                     /gene="TRIM50"
                     /gene_synonym="TRIM50A"
                     /inference="alignment:Splign:2.1.0"
     exon            184..600
                     /gene="TRIM50"
                     /gene_synonym="TRIM50A"
                     /inference="alignment:Splign:2.1.0"
     CDS             202..1665
                     /gene="TRIM50"
                     /gene_synonym="TRIM50A"
                     /EC_number="2.3.2.27"
                     /note="isoform a is encoded by transcript variant 1; E3
                     ubiquitin-protein ligase TRIM50; tripartite
                     motif-containing 50A; tripartite motif-containing protein
                     50; RING-type E3 ubiquitin transferase TRIM50"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase TRIM50 isoform a"
                     /protein_id="NP_835226.2"
                     /db_xref="CCDS:CCDS34654.1"
                     /db_xref="GeneID:135892"
                     /db_xref="HGNC:HGNC:19017"
                     /db_xref="MIM:612548"
                     /translation="MAWQVSLPELEDRLQCPICLEVFKEPLMLQCGHSYCKGCLVSLS
                     CHLDAELRCPVCRQAVDGSSSLPNVSLARVIEALRLPGDPEPKVCVHHRNPLSLFCEK
                     DQELICGLCGLLGSHQHHPVTPVSTVYSRMKEELAALISELKQEQKKVDELIAKLVNN
                     RTRIVNESDVFSWVIRREFQELHHLVDEEKARCLEGIGGHTRGLVASLDMQLEQAQGT
                     RERLAQAECVLEQFGNEDHHKFIRKFHSMASRAEMPQARPLEGAFSPISFKPGLHQAD
                     IKLTVWKRLFRKVLPAPEPLKLDPATAHPLLELSKGNTVVQCGLLAQRRASQPERFDY
                     STCVLASRGFSCGRHYWEVVVGSKSDWRLGVIKGTASRKGKLNRSPEHGVWLIGLKEG
                     RVYEAFACPRVPLPVAGHPHRIGLYLHYEQGELTFFDADRPDDLRPLYTFQADFQGKL
                     YPILDTCWHERGSNSLPMVLPPPSGPGPLSPEQPTKL"
     exon            601..696
                     /gene="TRIM50"
                     /gene_synonym="TRIM50A"
                     /inference="alignment:Splign:2.1.0"
     exon            697..927
                     /gene="TRIM50"
                     /gene_synonym="TRIM50A"
                     /inference="alignment:Splign:2.1.0"
     exon            928..950
                     /gene="TRIM50"
                     /gene_synonym="TRIM50A"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1075
                     /gene="TRIM50"
                     /gene_synonym="TRIM50A"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..2050
                     /gene="TRIM50"
                     /gene_synonym="TRIM50A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2019..2024
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRIM50"
                     /gene_synonym="TRIM50A"
                     /note="hexamer: AATAAA"
     polyA_site      2050
                     /gene="TRIM50"
                     /gene_synonym="TRIM50A"
                     /note="major polyA site"
ORIGIN      
        1 agagcatgat ggggcacgcg cggtagcgcg aggcggggca tgtaaccata gcgtgcgggt
       61 catgatgagg cacggacgtg gggggttagg tggggcacgt aattggagct cgcggggcag
      121 gatggggcat ctaactggag cgacagagag cacgatgggg cacttacagg ggccggaggc
      181 tggcccgggc agtgagtgtg gatggcttgg caggtgagcc tgccagagct ggaggaccgg
      241 cttcagtgtc ccatctgcct ggaggtcttc aaggagcccc tgatgctgca gtgtggccac
      301 tcttactgca agggctgcct ggtttccctg tcctgccacc tggatgccga gctgcgctgc
      361 cccgtgtgcc ggcaggcggt ggacggcagc agctccctgc ccaacgtctc cctggccagg
      421 gtgatcgaag ccctgaggct ccctggggac ccggagccca aggtctgcgt gcaccaccgg
      481 aacccgctca gccttttctg cgagaaggac caggagctca tctgtggcct ctgcggtctg
      541 ctgggctccc accaacacca cccggtcacg cccgtctcca ccgtctacag ccgcatgaag
      601 gaggagctcg cagccctcat ctctgagctg aagcaggagc agaaaaaggt ggatgagctc
      661 atcgccaaac tggtgaacaa ccggacccga atcgtcaatg agtcggatgt cttcagctgg
      721 gtgatccgcc gcgagttcca ggagctgcac cacctggtgg atgaggagaa ggcccgctgc
      781 ctggagggga tagggggtca cacccgtggc ctggtggcct ccctggacat gcagctggag
      841 caggcccagg gaacccggga gcggctggcc caagccgagt gtgtgctgga acagttcggc
      901 aatgaggacc accacaagtt catccggaag ttccactcca tggcctccag agcagagatg
      961 ccgcaggccc ggcccttaga aggcgcattc agccccatct ccttcaagcc aggcctccac
     1021 caggctgaca tcaagctgac cgtgtggaaa aggctcttcc ggaaagtttt gccagccccg
     1081 gagcctctca agttggaccc tgccactgcc cacccactcc tggagctctc caagggcaac
     1141 acggtggtgc agtgcgggct tctggcccag cggcgagcca gccagcctga gcgcttcgac
     1201 tacagcacct gcgtcctggc cagccgcggc ttctcctgcg gccgccacta ctgggaggtg
     1261 gtggtgggca gcaagagcga ctggcgcctg ggggtcatca agggcacagc cagccgtaag
     1321 ggcaagctga acaggtcccc cgagcacggc gtgtggctga tcggcctgaa ggagggccgg
     1381 gtgtacgaag cctttgcctg cccccgggta cccctgcccg tggccggcca cccccaccgc
     1441 atcgggctct acctgcacta tgagcagggc gaactcacct tcttcgatgc cgaccgcccc
     1501 gatgacctgc ggccgctcta caccttccag gccgacttcc agggcaagct ctaccccatc
     1561 ctggacacct gctggcacga gaggggcagc aactcgctgc ccatggtgct gcccccgccc
     1621 agcgggcctg gccccctcag ccccgagcag cccaccaagc tgtagggccg cccggagtcc
     1681 tgccggccca caggcccatc ccggcggggc accggggact cgcgggcttc tgctgggtcc
     1741 cgcctgaggt gatatcgtca ctgtttagga aggtcttcag gcctgtgtgt ccctggaaca
     1801 tgtaatgata gggaacagga ccccagtccc tttcccctca gccaagggtg tatttgtaaa
     1861 cttcggcccc caggccttgg cagccaggga cacactgaaa tccccatggt tcctgccact
     1921 tgctaaccat gctatctgcg cagacgcttc cgagggcaga aatagtccca tgtatacctt
     1981 ttccatatta ctgtgaactt tcaactacta tcaaggataa taaatttgac attatttctt
     2041 tctctttaaa
//
