LOCUS       NM_015906               8369 bp    mRNA    linear   PRI 24-OCT-2024
DEFINITION  Homo sapiens tripartite motif containing 33 (TRIM33), transcript
            variant a, mRNA.
ACCESSION   NM_015906
VERSION     NM_015906.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8369)
  AUTHORS   Xia,T., Meng,L., Xu,G., Sun,H. and Chen,H.
  TITLE     TRIM33 promotes glycolysis through regulating P53 K48-linked
            ubiquitination to promote esophageal squamous cell carcinoma growth
  JOURNAL   Cell Death Dis 15 (10), 740 (2024)
   PUBMED   39389957
  REMARK    GeneRIF: TRIM33 promotes glycolysis through regulating P53
            K48-linked ubiquitination to promote esophageal squamous cell
            carcinoma growth.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8369)
  AUTHORS   Ji,W., Zhang,W., Zhang,X. and Ke,Y.
  TITLE     TRIM33 enhances the ubiquitination of TFRC to enhance the
            susceptibility of liver cancer cells to ferroptosis
  JOURNAL   Cell Signal 121, 111268 (2024)
   PUBMED   38909931
  REMARK    GeneRIF: TRIM33 enhances the ubiquitination of TFRC to enhance the
            susceptibility of liver cancer cells to ferroptosis.
REFERENCE   3  (bases 1 to 8369)
  AUTHORS   McAvera,R.M., Morgan,J.J., Herrero,A.B., Mills,K.I. and
            Crawford,L.J.
  TITLE     TRIM33 loss in multiple myeloma is associated with genomic
            instability and sensitivity to PARP inhibitors
  JOURNAL   Sci Rep 14 (1), 8797 (2024)
   PUBMED   38627415
  REMARK    GeneRIF: TRIM33 loss in multiple myeloma is associated with genomic
            instability and sensitivity to PARP inhibitors.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8369)
  AUTHORS   Wu,Y., Yu,B., Ai,X., Zhang,W., Chen,W., Laurence,A., Zhang,M.,
            Chen,Q., Shao,Y. and Zhang,B.
  TITLE     TIF1gamma and SMAD4 regulation in colorectal cancer: impact on cell
            proliferation and liver metastasis
  JOURNAL   Biol Chem 405 (4), 241-256 (2024)
   PUBMED   38270141
  REMARK    GeneRIF: TIF1gamma and SMAD4 regulation in colorectal cancer:
            impact on cell proliferation and liver metastasis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8369)
  AUTHORS   Qi,Y., Ma,N. and Zhang,J.
  TITLE     Tripartite motif containing 33 demonstrated anticancer effect by
            degrading c-Myc: Limitation of glutamine metabolism and
            proliferation in endometrial carcinoma cells
  JOURNAL   Int J Oncol 63 (6) (2023)
   PUBMED   37859625
  REMARK    GeneRIF: Tripartite motif containing 33 demonstrated anticancer
            effect by degrading c-Myc: Limitation of glutamine metabolism and
            proliferation in endometrial carcinoma cells.
REFERENCE   6  (bases 1 to 8369)
  AUTHORS   He,W., Dorn,D.C., Erdjument-Bromage,H., Tempst,P., Moore,M.A. and
            Massague,J.
  TITLE     Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches
            of the TGFbeta pathway
  JOURNAL   Cell 125 (5), 929-941 (2006)
   PUBMED   16751102
  REMARK    GeneRIF: Thus, Smad2/3-TIF1gamma and Smad2/3-Smad4 function as
            complementary effector arms in the control of hematopoietic cell
            fate by the TGFbeta/Smad pathway.
REFERENCE   7  (bases 1 to 8369)
  AUTHORS   Peng,H., Feldman,I. and Rauscher,F.J. 3rd.
  TITLE     Hetero-oligomerization among the TIF family of RBCC/TRIM
            domain-containing nuclear cofactors: a potential mechanism for
            regulating the switch between coactivation and corepression
  JOURNAL   J Mol Biol 320 (3), 629-644 (2002)
   PUBMED   12096914
  REMARK    GeneRIF: TIF1alpha interacts with TIF1gamma and the coiled-coil
            region of TIF1gamma is necessary for this interaction.
REFERENCE   8  (bases 1 to 8369)
  AUTHORS   Reymond,A., Meroni,G., Fantozzi,A., Merla,G., Cairo,S., Luzi,L.,
            Riganelli,D., Zanaria,E., Messali,S., Cainarca,S., Guffanti,A.,
            Minucci,S., Pelicci,P.G. and Ballabio,A.
  TITLE     The tripartite motif family identifies cell compartments
  JOURNAL   EMBO J 20 (9), 2140-2151 (2001)
   PUBMED   11331580
REFERENCE   9  (bases 1 to 8369)
  AUTHORS   Klugbauer,S. and Rabes,H.M.
  TITLE     The transcription coactivator HTIF1 and a related protein are fused
            to the RET receptor tyrosine kinase in childhood papillary thyroid
            carcinomas
  JOURNAL   Oncogene 18 (30), 4388-4393 (1999)
   PUBMED   10439047
REFERENCE   10 (bases 1 to 8369)
  AUTHORS   Venturini,L., You,J., Stadler,M., Galien,R., Lallemand,V.,
            Koken,M.H., Mattei,M.G., Ganser,A., Chambon,P., Losson,R. and de
            The,H.
  TITLE     TIF1gamma, a novel member of the transcriptional intermediary
            factor 1 family
  JOURNAL   Oncogene 18 (5), 1209-1217 (1999)
   PUBMED   10022127
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL390241.19, CN267696.1,
            AF119043.1, AB029036.1, AK023434.1 and AL035410.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_015906.3.
            
            Summary: The protein encoded by this gene is thought to be a
            transcriptional corepressor. However, molecules that interact with
            this protein have not yet been identified. The protein is a member
            of the tripartite motif family. This motif includes three
            zinc-binding domains, a RING, a B-box type 1 and a B-box type 2,
            and a coiled-coil region. Three alternatively spliced transcript
            variants for this gene have been described, however, the
            full-length nature of one variant has not been determined.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (a) encodes the longer isoform
            (alpha).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF119043.1, AF220136.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000358465.7/ ENSP00000351250.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-43                AL390241.19        50980-51022         c
            44-80               CN267696.1         41-77
            81-1197             AF119043.1         38-1154
            1198-1272           AB029036.1         1084-1158
            1273-2645           AF119043.1         1230-2602
            2646-3550           AK023434.1         1816-2720
            3551-8369           AL035410.7         62673-67491
FEATURES             Location/Qualifiers
     source          1..8369
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p13.2"
     gene            1..8369
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /note="tripartite motif containing 33"
                     /db_xref="GeneID:51592"
                     /db_xref="HGNC:HGNC:16290"
                     /db_xref="MIM:605769"
     exon            1..653
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     CDS             128..3511
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /EC_number="2.3.2.27"
                     /note="isoform alpha is encoded by transcript variant a;
                     transcriptional intermediary factor 1 gamma; E3
                     ubiquitin-protein ligase TRIM33; TIF1-gamma; protein Rfg7;
                     ectodermin homolog; RET-fused gene 7 protein; RING-type E3
                     ubiquitin transferase TRIM33"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase TRIM33 isoform
                     alpha"
                     /protein_id="NP_056990.3"
                     /db_xref="CCDS:CCDS872.1"
                     /db_xref="GeneID:51592"
                     /db_xref="HGNC:HGNC:16290"
                     /db_xref="MIM:605769"
                     /translation="MAENKGGGEAESGGGGSGSAPVTAGAAGPAAQEAEPPLTAVLVE
                     EEEEEGGRAGAEGGAAGPDDGGVAAASSGSAQAASSPAASVGTGVAGGAVSTPAPAPA
                     SAPAPGPSAGPPPGPPASLLDTCAVCQQSLQSRREAEPKLLPCLHSFCLRCLPEPERQ
                     LSVPIPGGSNGDIQQVGVIRCPVCRQECRQIDLVDNYFVKDTSEAPSSSDEKSEQVCT
                     SCEDNASAVGFCVECGEWLCKTCIEAHQRVKFTKDHLIRKKEDVSESVGASGQRPVFC
                     PVHKQEQLKLFCETCDRLTCRDCQLLEHKEHRYQFLEEAFQNQKGAIENLLAKLLEKK
                     NYVHFAATQVQNRIKEVNETNKRVEQEIKVAIFTLINEINKKGKSLLQQLENVTKERQ
                     MKLLQQQNDITGLSRQVKHVMNFTNWAIASGSSTALLYSKRLITFQLRHILKARCDPV
                     PAANGAIRFHCDPTFWAKNVVNLGNLVIESKPAPGYTPNVVVGQVPPGTNHISKTPGQ
                     INLAQLRLQHMQQQVYAQKHQQLQQMRMQQPPAPVPTTTTTTQQHPRQAAPQMLQQQP
                     PRLISVQTMQRGNMNCGAFQAHQMRLAQNAARIPGIPRHSGPQYSMMQPHLQRQHSNP
                     GHAGPFPVVSVHNTTINPTSPTTATMANANRGPTSPSVTAIELIPSVTNPENLPSLPD
                     IPPIQLEDAGSSSLDNLLSRYISGSHLPPQPTSTMNPSPGPSALSPGSSGLSNSHTPV
                     RPPSTSSTGSRGSCGSSGRTAEKTSLSFKSDQVKVKQEPGTEDEICSFSGGVKQEKTE
                     DGRRSACMLSSPESSLTPPLSTNLHLESELDALASLENHVKIEPADMNESCKQSGLSS
                     LVNGKSPIRSLMHRSARIGGDGNNKDDDPNEDWCAVCQNGGDLLCCEKCPKVFHLTCH
                     VPTLLSFPSGDWICTFCRDIGKPEVEYDCDNLQHSKKGKTAQGLSPVDQRKCERLLLY
                     LYCHELSIEFQEPVPASIPNYYKIIKKPMDLSTVKKKLQKKHSQHYQIPDDFVADVRL
                     IFKNCERFNEMMKVVQVYADTQEINLKADSEVAQAGKAVALYFEDKLTEIYSDRTFAP
                     LPEFEQEEDDGEVTEDSDEDFIQPRRKRLKSDERPVHIK"
     exon            654..772
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            773..917
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            918..1050
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1167
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..1282
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1429
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..1547
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..1822
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            1823..1987
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            1988..2188
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            2189..2321
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            2322..2385
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            2386..2545
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            2546..2895
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            2896..3019
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            3020..3094
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            3095..3247
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            3248..3298
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            3299..8369
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5229..5234
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /note="hexamer: AAGAAA"
     polyA_site      5246
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /note="major polyA site"
     regulatory      8348..8353
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /note="hexamer: ATTAAA"
     polyA_site      8369
                     /gene="TRIM33"
                     /gene_synonym="ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;
                     TIFGAMMA"
                     /note="major polyA site"
ORIGIN      
        1 ggttcgctct ctcgctagct ctcggctgcc gctcttgctg cggctgcggc tggggctggg
       61 ggcggcggcg gcggcgacgc gggcggcggg cggcgcgggg cggtccggcg ggttcaaaga
      121 ggaaaacatg gcggaaaaca aaggcggcgg cgaggctgag agcggcggcg ggggcagcgg
      181 cagcgcgccg gtaactgccg gggccgccgg gcccgccgcg caggaggcgg agccgcctct
      241 caccgcggtg ctggtggagg aggaggagga ggaaggcggc agggccggcg ctgagggcgg
      301 cgcggccggg cccgacgacg ggggggtggc cgcggcctcc tcgggctcgg cccaggctgc
      361 ttcatctcct gcggcctcag tgggcactgg agttgccggg ggcgcagtat cgacgccggc
      421 tccagctcca gcctcggctc ccgctccggg tccctcggca gggccgcctc ctggaccgcc
      481 agcctcgctc ctggacacct gcgccgtgtg tcagcagagc ttgcagagcc ggcgtgaggc
      541 ggagcccaag ctgctgccct gtcttcactc cttctgcctg cgctgcctgc ccgagccgga
      601 gcgccagctc agcgtgccca tcccgggggg cagcaacggc gacatccagc aagttggtgt
      661 aatacggtgc ccagtatgcc gccaagaatg cagacagata gaccttgtgg ataattattt
      721 tgtgaaagac acatctgaag ctcctagcag ttctgatgaa aaatcagaac aggtatgtac
      781 tagttgtgaa gacaatgcaa gtgcagttgg cttttgtgta gaatgtggag agtggctatg
      841 taagacatgt atcgaagcac atcaaagagt aaaatttact aaagatcact tgatcaggaa
      901 gaaagaagat gtctcagagt ctgttggagc atctggtcaa cgccctgttt tctgccctgt
      961 acacaaacaa gaacagttga aacttttctg tgaaacatgt gatagattga catgtagaga
     1021 ctgtcagcta ttggaacaca aagaacatag gtatcagttt ttggaagaag cttttcaaaa
     1081 tcagaagggt gcaattgaga atctactggc gaaacttctt gagaagaaga attatgttca
     1141 ttttgcagct actcaggtgc agaataggat aaaagaagta aatgagacta acaaacgagt
     1201 agaacaggaa attaaagtgg ccattttcac ccttatcaat gaaattaata agaaaggaaa
     1261 atctctctta caacagctag agaatgttac aaaggaaaga cagatgaagt tactacagca
     1321 gcagaatgac atcacaggcc tttcccggca ggtgaagcat gttatgaact tcacaaattg
     1381 ggcaattgca agtggcagca gcacagcact actatacagc aagcgactga ttactttcca
     1441 gttgcgtcat attttgaaag cacggtgtga tcctgtccct gctgctaatg gagcaatacg
     1501 tttccattgt gatcccacct tctgggcaaa gaatgtagtc aatttaggta atctagtaat
     1561 agagagtaaa ccagctcctg gttatactcc taatgttgta gttgggcaag ttcctccagg
     1621 gacaaaccac attagtaaaa cccctggaca gattaactta gcacagcttc gactccagca
     1681 catgcaacaa caagtatatg cacagaaaca tcagcagttg caacagatga ggatgcagca
     1741 accaccagca cctgtaccaa ctacaacaac aacaacacaa cagcatccta gacaagcagc
     1801 ccctcagatg ttacaacaac agcctcctcg attgatcagt gtgcaaacaa tgcaaagagg
     1861 caacatgaac tgtggagctt ttcaagccca tcagatgaga ctggctcaga atgctgccag
     1921 aataccaggg atacccaggc acagcggccc tcaatattcc atgatgcagc cacacctcca
     1981 aagacaacac tcaaacccag ggcatgctgg accctttccc gtagtatcgg tacacaacac
     2041 cacaatcaac ccaacgagcc ctactacagc aactatggca aatgcaaacc gaggtcccac
     2101 cagcccatct gttacagcaa tagagctaat cccctcagtt accaatccag aaaaccttcc
     2161 atcgctgcca gatattccac ccatacagtt ggaagatgct ggctcaagta gtttagataa
     2221 tctactaagt agatacatct caggcagtca cctaccccca cagcctacaa gcaccatgaa
     2281 tccttctcca ggtccctctg ccctttctcc gggatcatca ggtttatcca attctcacac
     2341 acctgtgaga cccccaagta cttctagtac tggcagtcga ggcagctgtg ggtcatcagg
     2401 aagaactgct gagaagacaa gtcttagttt caaatctgat caggtgaagg tcaagcaaga
     2461 acctgggact gaagatgaaa tatgtagctt ttcaggaggt gtaaaacaag aaaaaacaga
     2521 ggatggcagg aggagtgctt gcatgttgag cagtcctgag agtagcttga caccacctct
     2581 ctcaaccaac ctgcatctag aaagtgaatt ggatgcattg gcaagcctgg aaaaccatgt
     2641 gaaaattgaa cctgcagata tgaatgaaag ctgcaaacag tcagggctca gcagccttgt
     2701 taatggaaag tccccaattc gaagcctcat gcacaggtcg gcaaggattg gaggagatgg
     2761 caacaataaa gatgatgacc caaatgaaga ctggtgtgct gtctgccaaa acggaggaga
     2821 tctcttgtgc tgcgaaaaat gtccaaaggt ctttcatcta acttgtcatg ttccaacact
     2881 acttagcttt ccaagtgggg actggatatg cacattttgt agagatattg gaaagccaga
     2941 agttgaatat gattgtgata atttgcaaca tagtaagaag gggaaaactg cgcaggggtt
     3001 aagccccgtg gaccaaagga aatgtgaacg tcttctgctt tacctctatt gccatgaatt
     3061 aagtattgaa ttccaggagc ctgttcctgc ttcgatacca aactactata aaattataaa
     3121 gaaaccaatg gatttatcca ccgtgaaaaa gaagcttcag aaaaaacatt cccaacacta
     3181 ccaaatcccg gatgactttg tggccgatgt ccgtttgatc ttcaagaact gtgaaaggtt
     3241 taatgaaatg atgaaagttg ttcaagttta tgcagacaca caagagatta atttgaaggc
     3301 tgattcagaa gtagctcagg cagggaaagc agttgcattg tactttgaag ataaactcac
     3361 agagatctac tcagacagga ccttcgcacc tttgccagag tttgagcagg aagaggatga
     3421 tggtgaggta actgaggact ctgatgaaga ctttatacag ccccgcagaa aacgcctaaa
     3481 gtcagatgag agaccagtac atataaagta aaatgacatg gatttaaatc aattgtttaa
     3541 aaaaaaaaaa acgaaaaaaa aaaaaaaaac acaaaaaacc cagaaaactt ttaagtgttg
     3601 ctggaatatc ctgcctacag tgggcacctc cttgaagaag ctgatagctt ttacacagta
     3661 ttagattgaa ataatggaca gaaacacatt cttgtcaaga aagggggaga gaagtctgtt
     3721 tgcaagtttc aaagcaaaaa gcaaaagtga aatgatttga ggatttctgt tctaatggag
     3781 atgattctct gattgttaga aatggcaaat attgatgatt gtgtgctatt gattggtgca
     3841 ggatacttgg tatacgagta aatacttgag actcgtgtca cttgataaat tttctttttg
     3901 gactaggtcg cacagttatt aaaacaactt ttaaccctcc cccttcacac acatacatat
     3961 caggttgttt tctagttaaa aacccaagta gctcagattc tactttaatg tcagtgcaga
     4021 tttgcattga atcatgccat tatgtttttt ctcattttta tgctgttggg tcttagtttt
     4081 taaattgata taaagaactc agcaatggtt ttattttcta ctcatactta gggtttagga
     4141 aacactacca ctagttatca tttaatcaac ttcaatggtc tactgaaaca aaaatggtaa
     4201 cttttcatta gtggattatt tagagttata gtagttgttt ccagaaaaca cttcctcaca
     4261 attgtacttc ccaatcaaat catgtgatca tacagttatt cccatgaaag gcagaatgtt
     4321 tgtttcaaaa ttaatctagt tttctgtaca tttaaatttg agaaggtgac aactggctct
     4381 tttccagtct tccttcatgt cagttttctg atagaccact attggcaaac agtatctgtc
     4441 aactaccaaa tgtgtaaaat tttctgtatt tcactttgtc ttatttgtaa atagtgaact
     4501 aaaacttttg gcagatcagc aacatttgct gagcctgttt tttaagctaa tgtgtattct
     4561 tactaatgtt cctatcaaga atggatttgt aatatatgct gtctatttct aatgttcaca
     4621 ttcatatttt gaggttctat cttattttaa tagagaacag acttctcaaa aaatcttcag
     4681 aagcagctta ttattgaaat atcgaaatat tgaaataaac ccggtggggt tagattactc
     4741 atctgtccac caagtgggac atttgcatgg actgggggct taaaggactt agaagagacc
     4801 tgtaagtaaa tcctgaaaat gagccaatcc ccacttgaat ggttactgga gtaaacccac
     4861 ctttaccacc ccaattacag cacccgaggc cgataaacca acttggctct ggttcatttt
     4921 tcttttcttc atttgtgatg ctcagattca aaatgtgtgt tctacactgt tacaggcttc
     4981 tcttttgttt gattaaagat tttagtccta cttttgtatg gacacattag aatattcaga
     5041 gaccaaaata gaagaatttg ctgttagata tttttcagaa gtcagcagat ttgtggcaaa
     5101 tcatttattt gcctttttaa aaattcattt aagcagttca gagagtagac tactcagaaa
     5161 attatttcac gtaattgtct aagaggtcaa tattttttaa tgcatattga atcaaataaa
     5221 gtgctctaaa gaaattatta tacaaattcc tttgggttgt ttttcttttc ttaacaaggg
     5281 gtgggggtaa acaggaatat gattcaggct ttctggttgt gtatttaaag agtattgatt
     5341 ttattattac tattgattta ctttattcct ggcttccttt tcacttttct ttcaattttt
     5401 aaaaaataat ttaagccgtt gaaaatatac caaactgttg aaacatttta ctcaaatttt
     5461 aaattcctaa aaatgttttt taataagagg gagaaaatta tttaaaaata cttatgccta
     5521 tgccaatttc cctctttttt cacaaaatcc atgatttcag tttgtaagta gacatatatc
     5581 taagggcaca tttttggaaa gtgaggaata gcagcagtat aacttcattt tgtcaggcct
     5641 ttgagttcta atattttgta ttgctcttca aatggatcct tttaaaaaaa ttgtagataa
     5701 tgagtcataa atagattctg ccaactgagg ggagaaacat tttaagtaaa tatttttcag
     5761 tatttggggc cttaaaaaat aattgtgttt ccttaaaatt acatgttaga tagagttttt
     5821 aggttttttt ggttttaaga ttggttaaag caatttaaaa gccacttttt tgtcaacatt
     5881 taatagcctc cacttctgtt aagataatgt atactgctga ggaattacta ttaatagcta
     5941 tcaacatacc accattaaat taaggtattc actttagatt ttttattaaa gcttttttct
     6001 tgcacactga tcgttgtgtt tctaagctga ttttttcagc tctaatatac ctatggttaa
     6061 aaagtataaa aacttaaatt gatatttaga tatatgtttt cctattagtt tatgttttaa
     6121 aaagacaaaa ttgtatctgt cagtccctga aggcagtttg tttttatact ctctcacatt
     6181 tgtatttgtt ttttaaatgg cagtatttta gaagatttgg agaaaagtcc acataataat
     6241 gttttcttaa aagcttttaa agtttttgct gtacttcaat ttacttcttc catcagaaaa
     6301 ctaagaacaa agtgttgctc agtctgttcc gctgacctaa atttgtgttt tcagcacttg
     6361 gctcagccaa ttcactgagt gaaggaattg ctttatgagg caaagcatgt gaaagttcta
     6421 aagtatggtt agattgtagg tcgtgctcta tatggaaaca tcaaaccatt actacagaga
     6481 aatgataagg cattggatcc actattgaaa ttattatttt tggatcaaca agttggtact
     6541 ttctgacttc tgtatcttaa cataagggaa ttttaggtaa tgctaagtca gttgtctcat
     6601 tttttgtgat aagttttgga atttttagtt aattgaaata aataatgctt ttaaatagaa
     6661 gtaaaaggtt tataagtgtg caaattgtag atttatcaat tacctcagca ggtatcctgc
     6721 catgtaatta ttagtgatta gtgttaataa gataatagat tcaggtcttc caactatgcc
     6781 cttggattgt ggcctactgt atgttattaa atggtctctt actatccaaa atgggagtag
     6841 atgctgtggc cccgtctccc ttggctttta cgtcccatat ccacccccat tcatgtacaa
     6901 catgtgaaat ataaaaatct catttcttgt caaaatcagc actgcttatt tgcatactca
     6961 gcatcggatc agtgagtagt tttataaaaa atccacgcac ccaactccct tagttaaaac
     7021 agattcttaa ttcataccat gaattcttaa tttctgtacc atctatgtta atgatctgct
     7081 gaaggtgact caagattttc aaggtgtaat acagtttgat catgtaccgg acctggatat
     7141 ttaatttttt ttccctcaca gttaatctcc tccttgataa agcaataaca ctgctttgag
     7201 tctgttgcct aatagcatgt cagaatcctc tcctggatgg tgattttata ggaaagtttg
     7261 tatgcatatc acccagtcta tcttttaaaa attaagaaat ttaaatgtat gctggaagta
     7321 atgacactat attgtggcat tttattttaa aaattgggga aagttgcata tttttttaaa
     7381 agtaagtgtt tgagtaaaaa aattgaaggt acttttttaa ggaaaaaaat ttatatgcca
     7441 cagtttacat agacatttca gattcaacac gtactcttga atataatggt ttcttttact
     7501 tggtcaaaat gcatgtatag catttctttc atcttagttc cttgtgtttg cctatgtggt
     7561 cctttatata ttttttattg tatcgaagaa acaaaactat cttcaaaaat aagttaattt
     7621 ggatatattt gtcatatcaa actacaaagt gtacaaagtt aagtttagcc cttttctaga
     7681 aagtgatctt taaaattaaa aatgctcctc ttttaaattc accaaattta tgtgtgggaa
     7741 ggcaccaaaa tgattttgta agtgccactg caatattccc tttcaagtgt ggcctaaatt
     7801 tcaatcttaa ggatggaatg catgtctgct ccttgttctg aaaaatgtag gcatctacta
     7861 cattttaaaa cacagtgaaa catatacata agcctataaa aaaagatttg tgcaatttga
     7921 aagcctgtta attttttatg tagacatacc tacacacgaa agggttaaat tcacagcctt
     7981 actagttcct tgcttccagt atttcaattg gtctcctccc ctcattatta ttattactac
     8041 tagtactatt atttttgcac atagttaact gcccttcaat atgattctta aaaagtgctg
     8101 tttctgtggt atcgtattct ctaaataatc atatttaatt ttttaaaaca aggttgcagt
     8161 ttctaattgt ttcgttcctg tgtttttgct ggtgtgtaat aaaagcaagt tttttctttt
     8221 catggttatt taatacatta gctgcctgta aataattctt gttataatgc tctggaatgt
     8281 gttgtagaag ttgtattaga ttagttttaa acccttgttt gaaagccaca ttgttttggt
     8341 tatttctatt aaattagaaa attgaaaaa
//
