LOCUS       NM_015905               8488 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens tripartite motif containing 24 (TRIM24), transcript
            variant 1, mRNA.
ACCESSION   NM_015905
VERSION     NM_015905.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8488)
  AUTHORS   Xu,C., Chen,G., Yu,B., Sun,B., Zhang,Y., Zhang,M., Yang,Y.,
            Xiao,Y., Cheng,S.Y., Li,Y. and Feng,H.
  TITLE     TRIM24 Cooperates with Ras Mutation to Drive Glioma Progression
            through snoRNA Recruitment of PHAX and DNA-PKcs
  JOURNAL   Adv Sci (Weinh) 11 (29), e2400023 (2024)
   PUBMED   38828688
  REMARK    GeneRIF: TRIM24 Cooperates with Ras Mutation to Drive Glioma
            Progression through snoRNA Recruitment of PHAX and DNA-PKcs.
REFERENCE   2  (bases 1 to 8488)
  AUTHORS   Ren,D., Li,W., Zeng,R., Liu,X., Liang,H., Xiong,W., Yang,C. and
            Jin,X.
  TITLE     Retinoblastoma-associated protein is important for TRIM24-mediated
            activation of the mTOR signaling pathway through DUSP2 action in
            prostate cancer
  JOURNAL   Cell Death Differ 31 (5), 592-604 (2024)
   PUBMED   38514847
  REMARK    GeneRIF: Retinoblastoma-associated protein is important for
            TRIM24-mediated activation of the mTOR signaling pathway through
            DUSP2 action in prostate cancer.
REFERENCE   3  (bases 1 to 8488)
  AUTHORS   Bardhan,I., Barman,S., Roy,A. and Sudhamalla,B.
  TITLE     Novel insights into the recognition of acetylated histone H4 tail
            by the TRIM24 PHD-Bromo module
  JOURNAL   Biochem J 480 (9), 629-647 (2023)
   PUBMED   37075063
  REMARK    GeneRIF: Novel insights into the recognition of acetylated histone
            H4 tail by the TRIM24 PHD-Bromo module.
            Erratum:[Biochem J. 2023 May 31;480(10):751. doi:
            10.1042/BCJ-2023-0011_COR.. PMID: 37255272]
REFERENCE   4  (bases 1 to 8488)
  AUTHORS   Wang,Y., Yao,Y., Wei,Q., Long,S., Chen,Y., Xie,J., Tan,R.,
            Jiang,W., Zhang,Q., Wu,D., Xiao,S., Wan,F. and Fu,K.
  TITLE     TRIM24 is critical for the cellular response to DNA double-strand
            breaks through regulating the recruitment of MRN complex
  JOURNAL   Oncogene 42 (8), 586-600 (2023)
   PUBMED   36550358
  REMARK    GeneRIF: TRIM24 is critical for the cellular response to DNA
            double-strand breaks through regulating the recruitment of MRN
            complex.
REFERENCE   5  (bases 1 to 8488)
  AUTHORS   Horvath,R.M., Dahabieh,M., Malcolm,T. and Sadowski,I.
  TITLE     TRIM24 controls induction of latent HIV-1 by stimulating
            transcriptional elongation
  JOURNAL   Commun Biol 6 (1), 86 (2023)
   PUBMED   36690785
  REMARK    GeneRIF: TRIM24 controls induction of latent HIV-1 by stimulating
            transcriptional elongation.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8488)
  AUTHORS   Venturini,L., You,J., Stadler,M., Galien,R., Lallemand,V.,
            Koken,M.H., Mattei,M.G., Ganser,A., Chambon,P., Losson,R. and de
            The,H.
  TITLE     TIF1gamma, a novel member of the transcriptional intermediary
            factor 1 family
  JOURNAL   Oncogene 18 (5), 1209-1217 (1999)
   PUBMED   10022127
REFERENCE   7  (bases 1 to 8488)
  AUTHORS   Fraser,R.A., Heard,D.J., Adam,S., Lavigne,A.C., Le Douarin,B.,
            Tora,L., Losson,R., Rochette-Egly,C. and Chambon,P.
  TITLE     The putative cofactor TIF1alpha is a protein kinase that is
            hyperphosphorylated upon interaction with liganded nuclear
            receptors
  JOURNAL   J Biol Chem 273 (26), 16199-16204 (1998)
   PUBMED   9632676
REFERENCE   8  (bases 1 to 8488)
  AUTHORS   Thenot,S., Henriquet,C., Rochefort,H. and Cavailles,V.
  TITLE     Differential interaction of nuclear receptors with the putative
            human transcriptional coactivator hTIF1
  JOURNAL   J Biol Chem 272 (18), 12062-12068 (1997)
   PUBMED   9115274
REFERENCE   9  (bases 1 to 8488)
  AUTHORS   Le Douarin,B., Nielsen,A.L., You,J., Chambon,P. and Losson,R.
  TITLE     TIF1 alpha: a chromatin-specific mediator for the ligand-dependent
            activation function AF-2 of nuclear receptors?
  JOURNAL   Biochem Soc Trans 25 (2), 605-612 (1997)
   PUBMED   9191165
  REMARK    Review article
REFERENCE   10 (bases 1 to 8488)
  AUTHORS   Moosmann,P., Georgiev,O., Le Douarin,B., Bourquin,J.P. and
            Schaffner,W.
  TITLE     Transcriptional repression by RING finger protein TIF1 beta that
            interacts with the KRAB repressor domain of KOX1
  JOURNAL   Nucleic Acids Res 24 (24), 4859-4867 (1996)
   PUBMED   9016654
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC008265.15, CB988362.1,
            BC028689.2, AK075306.1, AA844662.1 and AC013429.12.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_015905.2.
            
            Summary: The protein encoded by this gene mediates transcriptional
            control by interaction with the activation function 2 (AF2) region
            of several nuclear receptors, including the estrogen, retinoic
            acid, and vitamin D3 receptors. The protein localizes to nuclear
            bodies and is thought to associate with chromatin and
            heterochromatin-associated factors. The protein is a member of the
            tripartite motif (TRIM) family. The TRIM motif includes three
            zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 -
            and a coiled-coil region. Two alternatively spliced transcript
            variants encoding different isoforms have been described for this
            gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.252713.1, AK075306.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000343526.9/ ENSP00000340507.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-75                AC008265.15        50259-50333
            76-192              CB988362.1         105-221
            193-1439            BC028689.2         16-1262
            1440-1489           AK075306.1         1365-1414
            1490-4069           BC028689.2         1313-3892
            4070-4080           AA844662.1         3-13                c
            4081-8488           AC013429.12        66613-71020         c
FEATURES             Location/Qualifiers
     source          1..8488
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q33-q34"
     gene            1..8488
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /note="tripartite motif containing 24"
                     /db_xref="GeneID:8805"
                     /db_xref="HGNC:HGNC:11812"
                     /db_xref="MIM:603406"
     exon            1..654
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /inference="alignment:Splign:2.1.0"
     CDS             291..3443
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /EC_number="2.3.2.27"
                     /note="isoform a is encoded by transcript variant 1;
                     transcriptional intermediary factor 1; TIF1-alpha; RING
                     finger protein 82; E3 ubiquitin-protein ligase TRIM24;
                     RING-type E3 ubiquitin transferase TIF1-alpha"
                     /codon_start=1
                     /product="transcription intermediary factor 1-alpha
                     isoform a"
                     /protein_id="NP_056989.2"
                     /db_xref="CCDS:CCDS5847.1"
                     /db_xref="GeneID:8805"
                     /db_xref="HGNC:HGNC:11812"
                     /db_xref="MIM:603406"
                     /translation="MEVAVEKAVAAAAAASAAASGGPSAAPSGENEAESRQGPDSERG
                     GEAARLNLLDTCAVCHQNIQSRAPKLLPCLHSFCQRCLPAPQRYLMLPAPMLGSAETP
                     PPVPAPGSPVSGSSPFATQVGVIRCPVCSQECAERHIIDNFFVKDTTEVPSSTVEKSN
                     QVCTSCEDNAEANGFCVECVEWLCKTCIRAHQRVKFTKDHTVRQKEEVSPEAVGVTSQ
                     RPVFCPFHKKEQLKLYCETCDKLTCRDCQLLEHKEHRYQFIEEAFQNQKVIIDTLITK
                     LMEKTKYIKFTGNQIQNRIIEVNQNQKQVEQDIKVAIFTLMVEINKKGKALLHQLESL
                     AKDHRMKLMQQQQEVAGLSKQLEHVMHFSKWAVSSGSSTALLYSKRLITYRLRHLLRA
                     RCDASPVTNNTIQFHCDPSFWAQNIINLGSLVIEDKESQPQMPKQNPVVEQNSQPPSG
                     LSSNQLSKFPTQISLAQLRLQHMQQQVMAQRQQVQRRPAPVGLPNPRMQGPIQQPSIS
                     HQQPPPRLINFQNHSPKPNGPVLPPHPQQLRYPPNQNIPRQAIKPNPLQMAFLAQQAI
                     KQWQISSGQGTPSTTNSTSSTPSSPTITSAAGYDGKAFGSPMIDLSSPVGGSYNLPSL
                     PDIDCSSTIMLDNIVRKDTNIDHGQPRPPSNRTVQSPNSSVPSPGLAGPVTMTSVHPP
                     IRSPSASSVGSRGSSGSSSKPAGADSTHKVPVVMLEPIRIKQENSGPPENYDFPVVIV
                     KQESDEESRPQNANYPRSILTSLLLNSSQSSTSEETVLRSDAPDSTGDQPGLHQDNSS
                     NGKSEWLDPSQKSPLHVGETRKEDDPNEDWCAVCQNGGELLCCEKCPKVFHLSCHVPT
                     LTNFPSGEWICTFCRDLSKPEVEYDCDAPSHNSEKKKTEGLVKLTPIDKRKCERLLLF
                     LYCHEMSLAFQDPVPLTVPDYYKIIKNPMDLSTIKKRLQEDYSMYSKPEDFVADFRLI
                     FQNCAEFNEPDSEVANAGIKLENYFEELLKNLYPEKRFPKPEFRNESEDNKFSDDSDD
                     DFVQPRKKRLKSIEERQLLK"
     exon            655..773
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /inference="alignment:Splign:2.1.0"
     exon            774..921
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /inference="alignment:Splign:2.1.0"
     exon            922..1054
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1171
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /inference="alignment:Splign:2.1.0"
     exon            1172..1286
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..1433
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /inference="alignment:Splign:2.1.0"
     exon            1434..1551
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /inference="alignment:Splign:2.1.0"
     exon            1552..1820
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..1994
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /inference="alignment:Splign:2.1.0"
     exon            1995..2168
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /inference="alignment:Splign:2.1.0"
     exon            2169..2304
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /inference="alignment:Splign:2.1.0"
     exon            2305..2377
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /inference="alignment:Splign:2.1.0"
     exon            2378..2546
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /inference="alignment:Splign:2.1.0"
     exon            2547..2875
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /inference="alignment:Splign:2.1.0"
     exon            2876..3008
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /inference="alignment:Splign:2.1.0"
     exon            3009..3083
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /inference="alignment:Splign:2.1.0"
     exon            3084..3233
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /inference="alignment:Splign:2.1.0"
     exon            3234..8488
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4060..4065
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /note="hexamer: AAGAAA"
     polyA_site      4080
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /note="major polyA site"
     regulatory      4378..4383
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /note="hexamer: AATATA"
     polyA_site      4396
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
     polyA_site      5177
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
     regulatory      7838..7843
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /note="hexamer: AATAAA"
     polyA_site      7859
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
     regulatory      8463..8468
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
                     /note="hexamer: AATAAA"
     polyA_site      8488
                     /gene="TRIM24"
                     /gene_synonym="hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A;
                     TIF1ALPHA"
ORIGIN      
        1 gtattccacg agcgcctcgg cggttggcga agcggacggg gtgcagcctc cccggtgcga
       61 ggaacggtct ccgctgacag ataccctcct tccggccgcg ccactcggga ggcggatccc
      121 gtgggcctga ggaggcttcc cccgcccggt ttgctttccc tccctcgctg gcgctgccgc
      181 gagtccaccg agcggcctct gaggagcagc cgcaggagga ggaggaggtc gtcgggggcg
      241 gcgggcggag accgcgctct cgcttccccg gcggcggcaa gggcaggaca atggaggtgg
      301 cggtggagaa ggcggtggcg gcggcggcag cggcctcggc tgcggcctcc ggggggccct
      361 cggcggcgcc gagcggggag aacgaggccg agagtcggca gggcccggac tcggagcgcg
      421 gcggcgaggc ggcccggctc aacctgttgg acacttgcgc cgtgtgccac cagaacatcc
      481 agagccgggc gcccaagctg ctgccctgcc tgcactcttt ctgccagcgc tgcctgcccg
      541 cgccccagcg ctacctcatg ctgcccgcgc ccatgctggg ctcggccgag accccgccac
      601 ccgtccctgc ccccggctcg ccggtcagcg gctcgtcgcc gttcgccacc caagttggag
      661 tcattcgttg cccagtttgc agccaagaat gtgcagagag acacatcata gataactttt
      721 ttgtgaagga cactactgag gttcccagca gtacagtaga aaagtcaaat caggtatgta
      781 caagctgtga ggacaacgca gaagccaatg ggttttgtgt agagtgtgtt gaatggctct
      841 gcaagacgtg tatcagagct catcagaggg taaagttcac aaaagaccac actgtcagac
      901 agaaagagga agtatctcca gaggcagttg gtgtcaccag ccagcgacca gtgttttgtc
      961 cttttcataa aaaggagcag ctgaagctgt actgtgagac atgtgacaaa ctgacatgtc
     1021 gagactgtca gttgttagaa cataaagagc atagatacca atttatagaa gaagcttttc
     1081 agaatcagaa agtgatcata gatacactaa tcaccaaact gatggaaaaa acaaaataca
     1141 taaaattcac aggaaatcag atccaaaaca gaattattga agtaaatcaa aatcaaaagc
     1201 aggtggaaca ggatattaaa gttgctatat ttacactgat ggtagaaata aataaaaaag
     1261 gaaaagctct actgcatcag ttagagagcc ttgcaaagga ccatcgcatg aaacttatgc
     1321 aacaacaaca ggaagtggct ggactctcta aacaattgga gcatgtcatg catttttcta
     1381 aatgggcagt ttccagtggc agcagtacag cattacttta tagcaaacga ctgattacat
     1441 accggttacg gcacctcctt cgtgcaaggt gtgatgcatc cccagtgacc aacaacacca
     1501 tccaatttca ctgtgatcct agtttctggg ctcaaaatat catcaactta ggttctttag
     1561 taatcgagga taaagagagc cagccacaaa tgcctaagca gaatcctgtc gtggaacaga
     1621 attcacagcc accaagtggt ttatcatcaa accagttatc caagttccca acacagatca
     1681 gcctagctca attacggctc cagcatatgc agcaacaggt aatggctcag aggcaacagg
     1741 tgcaacggag gccagcacct gtgggtttac caaaccctag aatgcagggg cccatccagc
     1801 aaccttccat ctctcatcag caaccgcctc cacgtttgat aaactttcag aatcacagcc
     1861 ccaaacccaa tggaccagtt cttcctcctc atcctcaaca actgagatat ccaccaaacc
     1921 agaacatacc acgacaagca ataaagccaa accccctaca gatggctttc ttggctcaac
     1981 aagccataaa acagtggcag atcagcagtg gacagggaac cccatcaact accaacagca
     2041 catcctctac tccttccagc cccacgatta ctagtgcagc aggatatgat ggaaaggctt
     2101 ttggttcacc tatgatcgat ttgagctcac cagtgggagg gtcttataat cttccctctc
     2161 ttccggatat tgactgttca agtactatta tgctggacaa tattgtgagg aaagatacta
     2221 atatagatca tggccagcca agaccaccct caaacagaac ggtccagtca ccaaattcat
     2281 cagtgccatc tccaggcctt gcaggacctg ttactatgac tagtgtacac cccccaatac
     2341 gttcacctag tgcctccagc gttggaagcc gaggaagctc tggctcttcc agcaaaccag
     2401 caggagctga ctctacacac aaagtcccag tggtcatgct ggagccaatt cgaataaaac
     2461 aagaaaacag tggaccaccg gaaaattatg atttccctgt tgttatagtg aagcaagaat
     2521 cagatgaaga atctaggcct caaaatgcca attatccaag aagcatactc acctccctgc
     2581 tcttaaatag cagtcagagc tctacttctg aggagactgt gctaagatca gatgcccctg
     2641 atagtacagg agatcaacct ggacttcacc aggacaattc ctcaaatgga aagtctgaat
     2701 ggttggatcc ttcccagaag tcacctcttc atgttggaga gacaaggaaa gaggatgacc
     2761 ccaatgagga ctggtgtgca gtttgtcaaa acggagggga actcctctgc tgtgaaaagt
     2821 gccccaaagt attccatctt tcttgtcatg tgcccacatt gacaaatttt ccaagtggag
     2881 agtggatttg cactttctgc cgagacttat ctaaaccaga agttgaatat gattgtgatg
     2941 ctcccagtca caactcagaa aaaaagaaaa ctgaaggcct tgttaagtta acacctatag
     3001 ataaaaggaa gtgtgagcgc ctacttttat ttctttactg ccatgaaatg agcctggctt
     3061 ttcaagaccc tgttcctcta actgtgcctg attattacaa aataattaaa aatccaatgg
     3121 atttgtcaac catcaagaaa agactacaag aagattattc catgtactca aaacctgaag
     3181 attttgtagc tgattttaga ttgatctttc aaaactgtgc tgaattcaat gagcctgatt
     3241 cagaagtagc caatgctggt ataaaacttg aaaattattt tgaagaactt ctaaagaacc
     3301 tctatccaga aaaaaggttt cccaaaccag aattcaggaa tgaatcagaa gataataaat
     3361 ttagtgatga ttcagatgat gactttgtac agccccggaa gaaacgcctc aaaagcattg
     3421 aagaacgcca gttgcttaaa taatatgcag caccactagc ttgtgctggt ttttagattt
     3481 ttttgttttc aaaaaaacat ttgtcagtaa tttaacatca ctacaaaaag aagagtttgt
     3541 gactattctc atctctgttt tggacgttta ctagactttg atttccttaa tagcccattt
     3601 ctgttaacct cttatcacta agaaagaaag gaaagaagga gatgaataga agaaagaaaa
     3661 tggaaagaag gaaaaaagga ggatagaaaa aggatggaag aaagaagcat tgaaaacaaa
     3721 gacattcttc ccacttcttg gatttttaaa ccacagtctg gagtgatagc tactgtagaa
     3781 aggaaataga ctttgtatga actctttaag ttgaaaagta aaaaatatat gtggtttgga
     3841 tgtgtgcttt aattcagctt tagaaattaa taccactacc cgtgaattat atggcctgac
     3901 aatatgaatt aggtgtactg tactgaagaa cagtactcca caaacatggg tggtaacaag
     3961 agttccatcc caggaggcca aacggtgcaa cagaagggta ggttagatgc tattaagaag
     4021 gcacttaata gtacatcatg taagatggca actgtattaa agaaaaatcc ggaaaacaaa
     4081 tgtttgattt ttgtttttgt ttttatcttg tctgtagagg tattttggta tagcaggttt
     4141 tcaaggccat tttttataca tttctagatc tagattttca acttcttcca ctgagggaag
     4201 tatatacatt tgggtttgct gtgtgtctat gtgaggttta attgtacagg tgatcctttt
     4261 acaacaagcc tcattgtttg cagtatagct tttagtggaa ctacccaaaa tataaataca
     4321 gcagcgtgca ctgtatttga tgtgagggtt cttcatcata taccctactg ggcattaaat
     4381 ataagttcct ctgaaaggga ctcgtttttg tggttttcat ctgtctataa tttggaatga
     4441 aaatgtgttg taggattttg ggagcaggca gctgggggga attaatagtg attttttttt
     4501 tttcctgaag catctatctc atgtttttct tttgagagtc agaacatcaa acttaatctt
     4561 tgatctgact tctgatttta ttcttctgat tgattgatag aggtacaaaa gacttatctt
     4621 ctgaggacaa gcatattctt aatgtgccag acctacccgg ttcagctgat atagatagat
     4681 agatagatag aagaaaattg ctgtgccata cattaatcca gcatttgaca caatatctaa
     4741 atggtttgcc gaagttaatc tgtatttata aaacattaac tggagtaaat ttttctcctt
     4801 aggatgatag aataaaaaga gctcacttga aagaaggcta ttatttgcat tatatcacct
     4861 gccataaatt taacatagaa gacttggttt tatctacatg gctttacttc ttaaagtgga
     4921 aaatatcctg tggctaattc attgggtttt caggtactgc taaagataaa atacagggag
     4981 aaaataactt gttagcaata gatccccatt gtttatatat ataggtcttg ttcataatat
     5041 gtcaattatg tattgttaaa aagtcctact cacttttcaa atatgtgtta catggtaatg
     5101 tttgtcattg ttgttttaaa gttgcatttg acatttgttc tccaaagagt gtttgaacag
     5161 attttgataa cagtgcatac accttttgtc tttttttgct ccagcatttt atttttagca
     5221 cagcagctgt ggagcttttg ctgataattt tattgttggt atcttagaat actgttcgtc
     5281 tgacagttta ttcttccata cttgtctccc aagttaaaac agagcaaaac agataaatta
     5341 ttttgagaat catcacaaaa gggagttaga acttgtgaga ctaaaactat aatttcctaa
     5401 atgtgactaa tgaaccccaa actgtcacta ttacattcag tgccatattt atctttcaaa
     5461 acagtatttg tttcacccta accatctttg gctgaatgaa aggcagttga gaaccatctt
     5521 ttggtcatac ataatataat tatattaaaa gtaaagatcg gggctgggcg cagtggctca
     5581 cacctgcaat cccaccagca ctttgggagg ccaaggtggg cggatcacaa agtcaagagt
     5641 tcgagatcag cctggccaac attgtgaaac cccgcctcta caaaaaatac aaaaattagc
     5701 cggacatggt ggcgtgcaac tgtaatccca actactcagg aggctgagac aggagaatca
     5761 cttgaacgca ggaggcagaa gttgcagtga gccaagatct caccattgca ctccagcctg
     5821 agtgacagag actcagtctc aaaaacaaaa agtaaaggtc ttgggtttct ctctgtaaat
     5881 agcctttgtg taatggtctt tagattaata tcctttgact ttattaataa gtacctgtgt
     5941 gcccaggtgg atctcagcta agtcatcctt aaattctgtt caaatagaaa taaaccataa
     6001 aatgagccta actataaccc acactcactt atcaagcatt cccccaaaag ttcagtgcga
     6061 ttcaggtaac cttgacctaa acttgtacac tccggtgtac atctttgacc ataaactctg
     6121 ggagatttcc ctctggattt gggatcagga gcccagtagg gccccattgc tgaatgttta
     6181 tcacacttct ggctagggct tatggatgag cagaccacgt aggagctagg atttactcag
     6241 ttccacctca ctgacagctt ttctaggtgc caagcaactg acttttcctg tgtaaggaag
     6301 cccaagtact tgaccagtga tatatatggc catggcaaaa gtataaatgg atggatgtag
     6361 accatattca acgcagaata cattttagat cggtatctgg cattgtaatc attttgtctt
     6421 ttgaattaac atgccctgct tgtcttttct gaatggaagg gtaggaaagt ggaaggtact
     6481 aaaaaggaaa atgtagtttt agtttctttt ctaagctaaa gaactggctt tactactgct
     6541 atattttact accacatagt agtgtagatg tagtataact tgcttaggaa tgctttcaaa
     6601 aaggttacct ccatagtcaa aaaaaggggg agtatacagc tgattgttgc atttatgaaa
     6661 ctgcatttca atatgctatg ctgttactgt gcttagacac ttccttacgt ttgagttcca
     6721 aggagagaga gtgagaagag gaactccttt tgctgagctc tggtccaaat taatgtgaat
     6781 ccccaaagta aattgctgca tatgaacaac aggttttagg tttaaattga agatacattc
     6841 attttatatg tatttgttgt gaatgagaga aattgagtga tctgattggc cattaaaatt
     6901 atggtgaatg ggtcgggcgc agtggctcac gcctgtaatc ccagcacttt gggaggccga
     6961 ggtgggtaga tacctgaggt caggagtttg agaccagcct gaccaacatg gtgaaacccc
     7021 atctctacta aaaataccaa aatcaccagg gtgtggtggc ccatgcctgt aatcctagct
     7081 actcgggagg ctgaggcagg agaatcggct tgaacccagg aggcagaggt tgcagtgagc
     7141 tgagatcgca ccactgtagt ccagcctggg tgtcagagca agactctgtc acaaaaagaa
     7201 aaattatggg gaatggccgg gcgcggtggc tcacacctgt aatcccagca ctttgggagg
     7261 ccgaggtggg tggatcacct gaagtcagga gctcaagacc agcctggcca acatggtgaa
     7321 accccacctc aacagaatat acaaaaaaat tagctgggtg tggtggcggg tgcctgtaat
     7381 ctcagctact ttgggatgct gaggcaggag aatagcttga acccaggagg tggaggttgc
     7441 agtgagtcga gattgcacca ttgcactcca gcctgggcaa caagagcaaa actctgtctc
     7501 aaaaaaaaaa aaaaatggtg accatgcgtt caaccatata atttcttact gaaattgagc
     7561 tcctcctttt gaataaccat gtacatattt ttaaaagtat tttatagccc tatgtaaatt
     7621 aatagttgat gtctttttat aaatgtttat tgcatattca tcttgaatgt gaatttactg
     7681 ttgtaattca catactggtg tcatgtttct gagtgagaat aatgctagtc agtcagatca
     7741 cagatttttc tccatgaagg ttttacttat tgagagggac atctgaggca gtagtcttcg
     7801 tctttaattt ttgggggcag attggtgctt tggttttaat aaaaactaac tttgatggat
     7861 ttttactgtc aatattttac cacaaggatt ttttaataga aaaattactg aatgttactt
     7921 cagcagagtt ctatctactt tgagtctcca ttactactac tgttgttccc ctagctgtaa
     7981 cattgatcca tataggggca agctactagt ccataaatag ctgtaatagg aaaacagttt
     8041 tttaaaattc taacacgtgt atttcaccag ccttaaaaga tttaaaaaaa aatctgacag
     8101 gaaggaaagc gggtggtaag tatcttggat tattctgcct cataccagcc actcccaaag
     8161 taggaggacc aaagatgcca tgaagacttc tagttgtctg gaagttattt ggggaagggg
     8221 ggaaagacac agagtgggga cctttggaag tcattttaaa aatttagcta tgttaattcc
     8281 acttgtaaag tttgaaaggt tttgattctt gtaatttctg agaattacta tgttttaaat
     8341 ttactattgg atatttaaca gatctttcat ggggccaacc tatttgcttc cttaattttc
     8401 aaattaagtc ctaatttgag tcctaaaaaa atctttcctt gttagttgca agttaaacat
     8461 ttaataaaat tattttccca cgttttca
//
