LOCUS       NM_021158               2356 bp    mRNA    linear   PRI 19-AUG-2024
DEFINITION  Homo sapiens tribbles pseudokinase 3 (TRIB3), transcript variant 1,
            also known as 1A, mRNA.
ACCESSION   NM_021158
VERSION     NM_021158.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Lei,S., Sun,J., Xie,Y., Xiao,X., He,X., Lin,S., Zhang,H., Huang,Z.,
            Wang,H., Wu,X., Peng,H. and Liu,J.
  TITLE     Diverse functions of Tribbles homolog 3 in cancers and its
            potential as a therapeutic target
  JOURNAL   Carcinogenesis 45 (8), 527-542 (2024)
   PUBMED   38902892
  REMARK    GeneRIF: Diverse functions of Tribbles homolog 3 in cancers and its
            potential as a therapeutic target.
            Review article
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Meng,Z., Wang,Y., Wang,X. and Han,X.
  TITLE     TRIB3 promotes the growth of oral squamous cell carcinoma by
            regulating JNK/JUN-mediated aerobic glycolysis
  JOURNAL   Arch Oral Biol 164, 105977 (2024)
   PUBMED   38696945
  REMARK    GeneRIF: TRIB3 promotes the growth of oral squamous cell carcinoma
            by regulating JNK/JUN-mediated aerobic glycolysis.
REFERENCE   3  (bases 1 to 2356)
  AUTHORS   Li,J., Zhang,Q., Guan,Y., Liao,D., Chen,H., Xiong,H., Sheng,Y.,
            Chen,X. and Pang,J.
  TITLE     TRIB3 promotes the progression of renal cell carcinoma by
            upregulating the lipid droplet-associated protein PLIN2
  JOURNAL   Cell Death Dis 15 (4), 240 (2024)
   PUBMED   38561354
  REMARK    GeneRIF: TRIB3 promotes the progression of renal cell carcinoma by
            upregulating the lipid droplet-associated protein PLIN2.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2356)
  AUTHORS   Chen,L., Lin,W., Zhang,H., Geng,S., Le,Z., Wan,F., Huang,Q.,
            Chen,H., Liu,X., Lu,J.J. and Kong,L.
  TITLE     TRIB3 promotes malignancy of head and neck squamous cell carcinoma
            via inhibiting ferroptosis
  JOURNAL   Cell Death Dis 15 (3), 178 (2024)
   PUBMED   38429254
  REMARK    GeneRIF: TRIB3 promotes malignancy of head and neck squamous cell
            carcinoma via inhibiting ferroptosis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2356)
  AUTHORS   Ord,T., Ord,D., Koivomagi,M., Juhkam,K. and Ord,T.
  TITLE     Human TRB3 is upregulated in stressed cells by the induction of
            translationally efficient mRNA containing a truncated 5'-UTR
  JOURNAL   Gene 444 (1-2), 24-32 (2009)
   PUBMED   19505541
  REMARK    GeneRIF: increased transcription as well as altered usage of 5'-UTR
            variants contributes to the upregulation of hTRB3 protein synthesis
            in stressful conditions.
REFERENCE   6  (bases 1 to 2356)
  AUTHORS   Ord,D. and Ord,T.
  TITLE     Characterization of human NIPK (TRB3, SKIP3) gene activation in
            stressful conditions
  JOURNAL   Biochem Biophys Res Commun 330 (1), 210-218 (2005)
   PUBMED   15781252
  REMARK    GeneRIF: coexpression of hNIPK inhibits activation of hNIPK
            promoter in response to the stress-inducing agents and to
            overexpressed ATF4, and thus NIPK may function as a negative
            feedback regulator of ATF4
REFERENCE   7  (bases 1 to 2356)
  AUTHORS   Wu,M., Xu,L.G., Zhai,Z. and Shu,H.B.
  TITLE     SINK is a p65-interacting negative regulator of NF-kappaB-dependent
            transcription
  JOURNAL   J Biol Chem 278 (29), 27072-27079 (2003)
   PUBMED   12736262
  REMARK    GeneRIF: SINK specifically interacted with the NF-kappaB
            transactivator p65 and inhibited p65 phosphorylation
REFERENCE   8  (bases 1 to 2356)
  AUTHORS   Du,K., Herzig,S., Kulkarni,R.N. and Montminy,M.
  TITLE     TRB3: a tribbles homolog that inhibits Akt/PKB activation by
            insulin in liver
  JOURNAL   Science 300 (5625), 1574-1577 (2003)
   PUBMED   12791994
  REMARK    GeneRIF: results suggest that TRB3, a mammalian homolog of
            Drosophila tribbles, promotes glucose output from liver under
            fasting conditions by binding to and interfering with Akt
            phosphorylation in response to residual insulin signaling [Trb3
            tribbles homolog 3]
REFERENCE   9  (bases 1 to 2356)
  AUTHORS   Bowers,A.J., Scully,S. and Boylan,J.F.
  TITLE     SKIP3, a novel Drosophila tribbles ortholog, is overexpressed in
            human tumors and is regulated by hypoxia
  JOURNAL   Oncogene 22 (18), 2823-2835 (2003)
   PUBMED   12743605
  REMARK    GeneRIF: This protein, a novel Drosophila tribbles ortholog, is
            overexpressed in human tumors and is regulated by hypoxia.
REFERENCE   10 (bases 1 to 2356)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL034548.25.
            
            On Nov 5, 2020 this sequence version replaced NM_021158.4.
            
            Summary: The protein encoded by this gene is a putative protein
            kinase that is induced by the transcription factor NF-kappaB. The
            encoded protein is a negative regulator of NF-kappaB and can also
            sensitize cells to TNF- and TRAIL-induced apoptosis. In addition,
            this protein can negatively regulate the cell survival
            serine-threonine kinase AKT1. Differential promoter usage and
            alternate splicing result in multiple transcript variants.
            [provided by RefSeq, Jul 2014].
            
            Transcript Variant: This variant (1, also known as 1A) represents
            the longest transcript and encode the predominant isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC027484.1, AY247738.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000217233.9/ ENSP00000217233.3
            RefSeq Select criteria :: based on conservation, expression
            regulatory uORF        :: PMID: 19505541
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-410               AL034548.25        135438-135847
            411-701             AL034548.25        142689-142979
            702-994             AL034548.25        145965-146257
            995-2356            AL034548.25        150876-152237
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p13"
     gene            1..2356
                     /gene="TRIB3"
                     /gene_synonym="C20orf97; NIPK; SINK; SKIP3; TRB3"
                     /note="tribbles pseudokinase 3"
                     /db_xref="GeneID:57761"
                     /db_xref="HGNC:HGNC:16228"
                     /db_xref="MIM:607898"
     exon            1..410
                     /gene="TRIB3"
                     /gene_synonym="C20orf97; NIPK; SINK; SKIP3; TRB3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      46..279
                     /regulatory_class="uORF"
                     /gene="TRIB3"
                     /gene_synonym="C20orf97; NIPK; SINK; SKIP3; TRB3"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:19505541]"
                     /note="this uORF is predicted to encode a 77 aa peptide
                     (PMID:19505541)"
     regulatory      81..125
                     /regulatory_class="uORF"
                     /gene="TRIB3"
                     /gene_synonym="C20orf97; NIPK; SINK; SKIP3; TRB3"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:19505541]"
                     /note="this uORF is predicted to encode a 14 aa peptide
                     (PMID:19505541)"
     CDS             411..1487
                     /gene="TRIB3"
                     /gene_synonym="C20orf97; NIPK; SINK; SKIP3; TRB3"
                     /note="isoform 1 is encoded by transcript variant 1, also
                     known as 1A; p65-interacting inhibitor of NF-kappaB;
                     neuronal cell death inducible putative kinase; TRB-3;
                     p65-interacting inhibitor of NF-kappa-B; tribbles homolog
                     3"
                     /codon_start=1
                     /product="tribbles homolog 3 isoform 1"
                     /protein_id="NP_066981.2"
                     /db_xref="CCDS:CCDS12997.1"
                     /db_xref="GeneID:57761"
                     /db_xref="HGNC:HGNC:16228"
                     /db_xref="MIM:607898"
                     /translation="MRATPLAAPAGSLSRKKRLELDDNLDTERPVQKRARSGPQPRLP
                     PCLLPLSPPTAPDRATAVATASRLGPYVLLEPEEGGRAYQALHCPTGTEYTCKVYPVQ
                     EALAVLEPYARLPPHKHVARPTEVLAGTQLLYAFFTRTHGDMHSLVRSRHRIPEPEAA
                     VLFRQMATALAHCHQHGLVLRDLKLCRFVFADRERKKLVLENLEDSCVLTGPDDSLWD
                     KHACPAYVGPEILSSRASYSGKAADVWSLGVALFTMLAGHYPFQDSEPVLLFGKIRRG
                     AYALPAGLSAPARCLVRCLLRREPAERLTATGILLHPWLRQDPMPLAPTRSHLWEAAQ
                     VVPDGLGLDEAREEEGDREVVLYG"
     exon            411..701
                     /gene="TRIB3"
                     /gene_synonym="C20orf97; NIPK; SINK; SKIP3; TRB3"
                     /inference="alignment:Splign:2.1.0"
     exon            702..994
                     /gene="TRIB3"
                     /gene_synonym="C20orf97; NIPK; SINK; SKIP3; TRB3"
                     /inference="alignment:Splign:2.1.0"
     exon            995..2356
                     /gene="TRIB3"
                     /gene_synonym="C20orf97; NIPK; SINK; SKIP3; TRB3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2334..2339
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRIB3"
                     /gene_synonym="C20orf97; NIPK; SINK; SKIP3; TRB3"
                     /note="hexamer: AATAAA"
     polyA_site      2356
                     /gene="TRIB3"
                     /gene_synonym="C20orf97; NIPK; SINK; SKIP3; TRB3"
                     /note="major polyA site"
ORIGIN      
        1 agacgcccct agggggccag cgagggcggg tcccaggtgc agcggatgca gaggagagag
       61 gcccgggcgc ggcgcggggg atggtgcgat cccgggcccg agggcatcag acggcggctg
      121 attagctccg gtttgcatca cccggaccgg gggattagct ccggtttgca tcacccggac
      181 cgggggatta gctccggttt gcatcacccg gaccgggggc cgggcgcgca cgagactcgc
      241 agcggaagtg gaggcggctc cgcgcgcgtc cgctgctagg acccgggcag ggctggagct
      301 gggctgggat cccgagctcg gcagcagcgc agcgggccgg cccacctgct ggtgccctgg
      361 aggctctgag ccccggcggc gcccgggccc acgcggaacg acggggcgag atgcgagcca
      421 cccctctggc tgctcctgcg ggttccctgt ccaggaagaa gcggttggag ttggatgaca
      481 acttagatac cgagcgtccc gtccagaaac gagctcgaag tgggccccag cccagactgc
      541 ccccctgcct gttgcccctg agcccaccta ctgctccaga tcgtgcaact gctgtggcca
      601 ctgcctcccg tcttgggccc tatgtcctcc tggagcccga ggagggcggg cgggcctacc
      661 aggccctgca ctgccctaca ggcactgagt atacctgcaa ggtgtacccc gtccaggaag
      721 ccctggccgt gctggagccc tatgcgcggc tgcccccgca caagcatgtg gctcggccca
      781 ctgaggtcct ggctggtacc cagctcctct acgccttttt cactcggacc catggggaca
      841 tgcacagcct ggtgcgaagc cgccaccgta tccctgagcc tgaggctgcc gtgctcttcc
      901 gccagatggc caccgccctg gcgcactgtc accagcacgg tctggtcctg cgtgatctca
      961 agctgtgtcg ctttgtcttc gctgaccgtg agaggaagaa gctggtgctg gagaacctgg
     1021 aggactcctg cgtgctgact gggccagatg attccctgtg ggacaagcac gcgtgcccag
     1081 cctacgtggg acctgagata ctcagctcac gggcctcata ctcgggcaag gcagccgatg
     1141 tctggagcct gggcgtggcg ctcttcacca tgctggccgg ccactacccc ttccaggact
     1201 cggagcctgt cctgctcttc ggcaagatcc gccgcggggc ctacgccttg cctgcaggcc
     1261 tctcggcccc tgcccgctgt ctggttcgct gcctccttcg tcgggagcca gctgaacggc
     1321 tcacagccac aggcatcctc ctgcacccct ggctgcgaca ggacccgatg cccttagccc
     1381 caacccgatc ccatctctgg gaggctgccc aggtggtccc tgatggactg gggctggacg
     1441 aagccaggga agaggaggga gacagagaag tggttctgta tggctaggac caccctacta
     1501 cacgctcagc tgccaacagt ggattgagtt tgggggtagc tccaagcctt ctcctgcctc
     1561 tgaactgagc caaaccttca gtgccttcca gaagggagaa aggcagaagc ctgtgtggag
     1621 tgtgctgtgt acacatctgc tttgttccac acacatgcag ttcctgcttg ggtgcttatc
     1681 aggtgccaag ccctgttctc ggtgctggga gtacagcagt gagcaaagga gacaatattc
     1741 cctgctcaca gagatgacaa actggcatcc ttgagctgac aacacttttc catgaccata
     1801 ggtcactgtc tacactgggt acactttgta ccagtgtcgg cctccactga tgctggtgct
     1861 caggcacctc tgtccaagga caatcccttt cacaaacaaa ccagctgcct ttgtatcttg
     1921 taccttttca gagaaaggga ggtatccctg tgccaaaggc tccaggcctc tcccctgcaa
     1981 ctcaggaccc aagcccagct cactctggga actgtgttcc cagcatctct gtcctcttga
     2041 ttaagagatt ctccttccag gcctaagcct gggatttggg ccagagataa gaatccaaac
     2101 tatgaggcta gttcttgtct aactcaagac tgttctggaa tgagggtcca ggcctgtcaa
     2161 ccatggggct tctgacctga gcaccaaggt tgagggacag gattaggcag ggtctgtcct
     2221 gtggccacct ggaaagtccc aggtgggact cttctgggga cacttggggt ccacaatccc
     2281 aggtccatac tctaggtttt ggataccatg agtatgtatg tttacctgtg cctaataaag
     2341 gagaattatg aaataa
//
