LOCUS       NM_001160372            6899 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens trafficking protein particle complex subunit 9
            (TRAPPC9), transcript variant 2, mRNA.
ACCESSION   NM_001160372
VERSION     NM_001160372.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Kharrat,M., Triki,C., Ben Isaa,A., Bouchaala,W., Alila,O.,
            Chouchen,J., Ghouliya,Y., Kamoun,F., Tlili,A. and Fakhfakh,F.
  TITLE     Expanding the genetic and phenotypic spectrum of TRAPPC9 and
            MID2-related neurodevelopmental disabilities: report of two novel
            mutations, 3D-modelling, and molecular docking studies
  JOURNAL   J Hum Genet 69 (7), 291-299 (2024)
   PUBMED   38467738
  REMARK    GeneRIF: Expanding the genetic and phenotypic spectrum of TRAPPC9
            and MID2-related neurodevelopmental disabilities: report of two
            novel mutations, 3D-modelling, and molecular docking studies.
REFERENCE   2  (bases 1 to 6899)
  AUTHORS   Lorente-Pozo,S., Boronat,N., Parra-Llorca,A., Lara-Canton,I.,
            Solaz-Garcia,A., Oltra Soler,J.V., Buso,E.J., Casabo-Valles,G.,
            Garcia-Gimenez,J.L., Pallardo,F.V. and Vento,M.
  TITLE     Neurodevelopmental Outcome and Epigenetic Changes at 2 Years
            Associated with the Oxygen Load Received upon Postnatal
            Stabilization: A Pilot Study
  JOURNAL   Neonatology 119 (5), 575-584 (2022)
   PUBMED   35760056
  REMARK    GeneRIF: Neurodevelopmental Outcome and Epigenetic Changes at 2
            Years Associated with the Oxygen Load Received upon Postnatal
            Stabilization: A Pilot Study.
REFERENCE   3  (bases 1 to 6899)
  AUTHORS   Ben Ayed,I., Bouchaala,W., Bouzid,A., Feki,W., Souissi,A., Ben
            Nsir,S., Ben Said,M., Sammouda,T., Majdoub,F., Kharrat,I.,
            Kamoun,F., Elloumi,I., Kamoun,H., Tlili,A., Masmoudi,S. and
            Triki,C.
  TITLE     Further insights into the spectrum phenotype of TRAPPC9 and
            CDK5RAP2 genes, segregating independently in a large Tunisian
            family with intellectual disability and microcephaly
  JOURNAL   Eur J Med Genet 64 (12), 104373 (2021)
   PUBMED   34737153
  REMARK    GeneRIF: Further insights into the spectrum phenotype of TRAPPC9
            and CDK5RAP2 genes, segregating independently in a large Tunisian
            family with intellectual disability and microcephaly.
REFERENCE   4  (bases 1 to 6899)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 6899)
  AUTHORS   Alvarez-Mora,M.I., Corominas,J., Gilissen,C., Sanchez,A.,
            Madrigal,I. and Rodriguez-Revenga,L.
  TITLE     Novel Compound Heterozygous Mutation in TRAPPC9 Gene: The Relevance
            of Whole Genome Sequencing
  JOURNAL   Genes (Basel) 12 (4), 557 (2021)
   PUBMED   33921338
  REMARK    GeneRIF: Novel Compound Heterozygous Mutation in TRAPPC9 Gene: The
            Relevance of Whole Genome Sequencing.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6899)
  AUTHORS   Kent,J.W. Jr., Peterson,C.P., Dyer,T.D., Almasy,L. and Blangero,J.
  TITLE     Genome-wide discovery of maternal effect variants
  JOURNAL   BMC Proc 3 Suppl 7 (Suppl 7), S19 (2009)
   PUBMED   20018008
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 6899)
  AUTHORS   Mir,A., Kaufman,L., Noor,A., Motazacker,M.M., Jamil,T., Azam,M.,
            Kahrizi,K., Rafiq,M.A., Weksberg,R., Nasr,T., Naeem,F.,
            Tzschach,A., Kuss,A.W., Ishak,G.E., Doherty,D., Ropers,H.H.,
            Barkovich,A.J., Najmabadi,H., Ayub,M. and Vincent,J.B.
  TITLE     Identification of mutations in TRAPPC9, which encodes the NIK- and
            IKK-beta-binding protein, in nonsyndromic autosomal-recessive
            mental retardation
  JOURNAL   Am J Hum Genet 85 (6), 909-915 (2009)
   PUBMED   20004765
  REMARK    GeneRIF: TRAPPC9, encodes the NIK- and IKK-beta-binding protein,
            has a role in nonsyndromic autosomal-recessive mental retardation
REFERENCE   8  (bases 1 to 6899)
  AUTHORS   Philippe,O., Rio,M., Carioux,A., Plaza,J.M., Guigue,P.,
            Molinari,F., Boddaert,N., Bole-Feysot,C., Nitschke,P., Smahi,A.,
            Munnich,A. and Colleaux,L.
  TITLE     Combination of linkage mapping and microarray-expression analysis
            identifies NF-kappaB signaling defect as a cause of
            autosomal-recessive mental retardation
  JOURNAL   Am J Hum Genet 85 (6), 903-908 (2009)
   PUBMED   20004764
REFERENCE   9  (bases 1 to 6899)
  AUTHORS   Mochida,G.H., Mahajnah,M., Hill,A.D., Basel-Vanagaite,L.,
            Gleason,D., Hill,R.S., Bodell,A., Crosier,M., Straussberg,R. and
            Walsh,C.A.
  TITLE     A truncating mutation of TRAPPC9 is associated with
            autosomal-recessive intellectual disability and postnatal
            microcephaly
  JOURNAL   Am J Hum Genet 85 (6), 897-902 (2009)
   PUBMED   20004763
  REMARK    GeneRIF: TRAPPC9 has a role in brain development, possibly through
            its effect on NF-kappaB activation and protein trafficking in the
            postmitotic neurons of the cerebral cortex
REFERENCE   10 (bases 1 to 6899)
  AUTHORS   Hu,W.H., Pendergast,J.S., Mo,X.M., Brambilla,R., Bracchi-Ricard,V.,
            Li,F., Walters,W.M., Blits,B., He,L., Schaal,S.M. and Bethea,J.R.
  TITLE     NIBP, a novel NIK and IKK(beta)-binding protein that enhances
            NF-(kappa)B activation
  JOURNAL   J Biol Chem 280 (32), 29233-29241 (2005)
   PUBMED   15951441
  REMARK    GeneRIF: NIBP is a NIK and IKK(beta)-binding protein that enhances
            NF-(kappa)B activation
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA228705.1, DA778234.1,
            AB067469.1, AC091022.4, AC040978.8, AC007869.2 and R49592.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001160372.3.
            
            Summary: This gene encodes a protein that likely plays a role in
            NF-kappa-B signaling. Mutations in this gene have been associated
            with autosomal-recessive cognitive disability. Alternatively
            spliced transcript variants have been described.[provided by
            RefSeq, Feb 2010].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB067469.1, AK026394.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000438773.4/ ENSP00000405060.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 DA228705.1         19-21
            4-527               DA778234.1         4-527
            528-1150            AB067469.1         496-1118
            1151-1151           AC091022.4         74818-74818
            1152-2914           AB067469.1         1120-2882
            2915-2915           AC040978.8         53298-53298         c
            2916-4279           AB067469.1         2884-4247
            4280-6675           AC007869.2         6197-8592
            6676-6899           R49592.1           8-231               c
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.3"
     gene            1..6899
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /note="trafficking protein particle complex subunit 9"
                     /db_xref="GeneID:83696"
                     /db_xref="HGNC:HGNC:30832"
                     /db_xref="MIM:611966"
     exon            1..106
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            107..700
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     CDS             117..3563
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /note="isoform b is encoded by transcript variant 2; IKK2
                     binding protein; TRAPP 120 kDa subunit; NIK- and
                     IKBKB-binding protein; NIK and IKK-beta binding protein;
                     tularik gene 1 protein; trafficking protein particle
                     complex 9"
                     /codon_start=1
                     /product="trafficking protein particle complex subunit 9
                     isoform b"
                     /protein_id="NP_001153844.1"
                     /db_xref="CCDS:CCDS55278.1"
                     /db_xref="GeneID:83696"
                     /db_xref="HGNC:HGNC:30832"
                     /db_xref="MIM:611966"
                     /translation="MSVPDYMQCAEDHQTLLVVVQPVGIVSEENFFRIYKRICSVSQI
                     SVRDSQRVLYIRYRHHYPPENNEWGDFQTHRKVVGLITITDCFSAKDWPQTFEKFHVQ
                     KEIYGSTLYDSRLFVFGLQGEIVEQPRTDVAFYPNYEDCQTVEKRIEDFIESLFIVLE
                     SKRLDRATDKSGDKIPLLCVPFEKKDFVGLDTDSRHYKKRCQGRMRKHVGDLCLQAGM
                     LQDSLVHYHMSVELLRSVNDFLWLGAALEGLCSASVIYHYPGGTGGKSGARRFQGSTL
                     PAEAANRHRPGAQEVLIDPGALTTNGINPDTSTEIGRAKNCLSPEDIIDKYKEAISYY
                     SKYKNAGVIELEACIKAVRVLAIQKRSMEASEFLQNAVYINLRQLSEEEKIQRYSILS
                     ELYELIGFHRKSAFFKRVAAMQCVAPSIAEPGWRACYKLLLETLPGYSLSLDPKDFSR
                     GTHRGWAAVQMRLLHELVYASRRMGNPALSVRHLSFLLQTMLDFLSDQEKKDVAQSLE
                     NYTSKCPGTMEPIALPGGLTLPPVPFTKLPIVRHVKLLNLPASLRPHKMKSLLGQNVS
                     TKSPFIYSPIIAHNRGEERNKKIDFQWVQGDVCEVQLMVYNPMPFELRVENMGLLTSG
                     VEFESLPAALSLPAESGLYPVTLVGVPQTTGTITVNGYHTTVFGVFSDCLLDNLPGIK
                     TSGSTVEVIPALPRLQISTSLPRSAHSLQPSSGDEISTNVSVQLYNGESQQLIIKLEN
                     IGMEPLEKLEVTSKVLTTKEKLYGDFLSWKLEETLAQFPLQPGKVATFTINIKVKLDF
                     SCQENLLQDLSDDGISVSGFPLSSPFRQVVRPRVEGKPVNPPESNKAGDYSHVKTLEA
                     VLNFKYSGGPGHTEGYYRNLSLGLHVEVEPSVFFTRVSTLPATSTRQCHLLLDVFNST
                     EHELTVSTRSSEALILHAGECQRMAIQVDKFNFESFPESPGEKGQFANPKQLEEERRE
                     ARGLEIHSKLGICWRIPSLKRSGEASVEGLLNQLVLEHLQLAPLQWDVLVDGQPCDRE
                     AVAACQVGDPVRLEVRLTNRSPRSVGPFALTVVPFQDHQNGVHNYDLHDTVSFVGSST
                     FYLDAVQPSGQSACLGALLFLYTGDFFLHIRFHEDSTSKELPPSWFCLPSVHVCALEA
                     QA"
     exon            701..846
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            847..975
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1002
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1124
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1250
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..1467
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1611
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            1612..1738
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            1739..1884
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            1885..1970
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            1971..2097
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            2098..2230
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            2231..2394
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            2395..2547
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            2548..2672
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            2673..2815
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            2816..2926
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            2927..3080
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            3081..3171
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            3172..3395
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     exon            3396..6899
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4259..4264
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /note="hexamer: AATAAA"
     polyA_site      4279
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /note="major polyA site"
     regulatory      6520..6525
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /note="hexamer: AATAAA"
     regulatory      6530..6535
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /note="hexamer: GATAAA"
     polyA_site      6539
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
     polyA_site      6552
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
     regulatory      6620..6625
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
                     /note="hexamer: AATAAA"
     polyA_site      6644
                     /gene="TRAPPC9"
                     /gene_synonym="IBP; IKBKBBP; MRT13; NIBP; T1; TRS120"
ORIGIN      
        1 agtaggaagc gccagcccag agcctgggaa gtggccgcag aggctgctcg gccccgctgc
       61 ccgccaggtc gtgggctccc gctccgggcc cggggccacc ggctgtggac ttcaaaatga
      121 gcgtccctga ctacatgcag tgtgctgagg accaccagac gctgctcgtg gtggtccagc
      181 ctgtgggcat cgtctccgag gagaacttct tcaggatcta taagaggatt tgctctgtga
      241 gtcagatcag cgtgcgggac tcccagcgag tcctctacat ccgctacagg caccactacc
      301 cacccgagaa caacgagtgg ggtgacttcc agacccaccg caaagtcgtg ggcctcatca
      361 ccatcacaga ctgcttctcg gccaaggact ggccacagac ctttgagaag ttccacgtgc
      421 agaaggagat ctacggctcc acactgtatg actcccggct ctttgtcttc gggctgcagg
      481 gggagatcgt ggagcagccg cgcaccgacg tggctttcta ccccaactac gaggactgcc
      541 agacggtgga gaagagaatc gaggacttca tcgagtcact gttcatcgtg ctggagtcca
      601 agcgtctgga cagagccaca gacaagtctg gggataagat cccccttctc tgtgtcccgt
      661 ttgagaaaaa ggactttgta ggactggaca cagacagcag acattacaag aagcggtgcc
      721 aaggccgcat gcggaagcac gtgggggacc tgtgcctgca ggcagggatg ctgcaggact
      781 ccctggtgca ttaccacatg tcggtggagc tgctgcgttc tgtgaatgac tttctgtggc
      841 ttggagctgc cctggaagga ttgtgttcag cttctgtcat ctatcactat cctggtggaa
      901 ctggtgggaa gagtggagct cggaggttcc agggcagcac ccttcctgct gaagcagcca
      961 atagacaccg gccaggggca caggaagttc tcattgatcc aggtgccctc accaccaatg
     1021 gcatcaaccc tgacaccagt actgagatcg gacgtgctaa gaactgcctt agccctgaag
     1081 acataattga caagtataaa gaggcgattt cctattacag caagtataag aatgcgggag
     1141 tgattgagtt ggaagcgtgc atcaaggctg tacgtgtcct tgcaattcag aaacggagca
     1201 tggaagcatc agaatttctt cagaatgcag tttacattaa ccttcgacag ctttctgagg
     1261 aagagaaaat tcagcgctac agcatcctct ccgagctcta tgagctgatc ggcttccatc
     1321 gcaagtctgc gttcttcaag cgcgtggccg ccatgcagtg cgtggcccca agcatcgcgg
     1381 agcctgggtg gagggcctgc tacaaactcc tcctggaaac gctgcccggc tacagtctgt
     1441 cgctggatcc caaagatttc agcagaggca cgcacagagg ctgggctgcg gtccagatgc
     1501 gtttgctcca tgaattggtc tacgcctccc gaaggatggg gaaccctgcc ctctctgtca
     1561 gacacctgtc cttccttcta cagaccatgc tggacttctt gtcggatcag gaaaagaaag
     1621 atgtggccca aagcctagag aactatacgt ccaagtgtcc tgggaccatg gagcccatcg
     1681 ccctccctgg cggcctcacc ctgccaccgg tgcccttcac caagcttccc atcgtcaggc
     1741 atgtgaaact attgaacctt cctgctagcc tccggccaca caaaatgaaa agcttgctgg
     1801 gtcagaacgt gtcaaccaaa agtcctttca tctattcacc aattatcgca cacaaccgtg
     1861 gagaagagcg gaacaagaaa atagatttcc agtgggttca aggagatgtg tgtgaagttc
     1921 agctgatggt atataaccca atgccgtttg aacttcgagt tgaaaacatg gggctgctca
     1981 ccagcggagt ggagttcgag tctctccctg cggcgctttc tcttccggct gaatctggtc
     2041 tgtacccagt gacgctcgtc ggggtcccgc agacgactgg aacgattact gtgaacggtt
     2101 accataccac ggtcttcggt gtgttcagtg actgtttgct ggataacctg ccgggaataa
     2161 aaaccagtgg ctccacagtg gaagtcattc ccgcgttgcc aagactgcag atcagcacct
     2221 ctctgcccag atctgcacat tcattgcaac cttcttctgg tgatgaaata tctactaatg
     2281 tatctgtcca gctttacaat ggagaaagtc agcaactaat cattaaattg gaaaatattg
     2341 gaatggaacc attggagaaa ctggaggtca cctcgaaagt tctcaccact aaagaaaaat
     2401 tgtatggcga cttcttgagc tggaagctag aggaaaccct tgcccagttc cctttgcagc
     2461 ctgggaaggt ggccacgttc acaatcaaca tcaaagtgaa gctggatttc tcctgccagg
     2521 agaatctcct gcaggatctc agtgatgatg gaatcagtgt gagtggcttt cccctgtcca
     2581 gtccttttcg gcaggtcgtt cggccccgag tggagggcaa acctgtgaac ccacccgaga
     2641 gcaacaaagc aggcgactac agccacgtga agaccctgga agctgtcctg aatttcaaat
     2701 actctggagg cccgggccac actgaaggat attacaggaa tctctccctg gggctgcatg
     2761 tagaagtcga gccgtctgta tttttcaccc gagtcagcac cctcccagca accagtaccc
     2821 ggcagtgtca cctgctcctg gatgtcttca actccaccga gcatgagctg accgtcagca
     2881 ccaggagcag cgaggcactc atcctgcacg ccggtgagtg ccagcgaatg gctattcaag
     2941 tggacaagtt caactttgag agtttcccgg agtcccctgg ggagaagggg caatttgcaa
     3001 accccaagca gctggaggaa gagcggcggg aagcccgagg cctggagatc cacagcaagc
     3061 tgggcatctg ctggagaatc ccctccctga agcgcagtgg cgaggcgagt gtggaaggac
     3121 tcctgaacca gctcgtcctg gagcacctgc agctggcgcc tctgcagtgg gatgtgctgg
     3181 tggacggaca gccatgtgac cgcgaggctg tggcggcctg ccaggtgggc gaccccgtgc
     3241 gcctggaggt gcggctgacc aaccggagcc cgcgcagcgt agggcccttc gccctcactg
     3301 tggtcccctt ccaggaccac cagaacggcg tgcacaacta cgacctgcac gacaccgtct
     3361 ccttcgtggg ctccagcacc ttctacctcg acgcggtgca gccgtccggc cagtcggcct
     3421 gcctcggggc cctcctcttc ctctacacgg gagacttctt cctccacatc cggttccacg
     3481 aggacagcac cagcaaggag ctgccaccct cttggttctg cctgcccagt gtgcacgtgt
     3541 gtgccctgga ggcgcaggcc tgagcccgcc tacttccgtc cctctttctg cagggccaga
     3601 ggtgaccctg cctggcctcc cacaccccct gcaatgagca aggccttcac tgcagcccca
     3661 tctcctcctc ctcccccaga cccctcccag ccctctcctc ctgttcctcc tgtagcatct
     3721 ttgctgggct acgcagaagc cccggacatg gcagccccac cccatgccac gccccttcct
     3781 acactgttcc ctggaccata cacaggctga agcagaggaa atcccaaagc gggtgcccat
     3841 ccagcccagg tcccaggatc cctgcaccca tttctgtgac ctggggcccc agccgtgctg
     3901 tgctgctcat cccagcagag ggacctccct cgtccagcga cttccctttg gccatagaaa
     3961 gaaatggtga gcatgagact gggcacagcc tgagggcgtg ggcagcttcc caccctccct
     4021 gggccttgga atcccccaag gctggttttc ttcctggaga cccccatggg caacttggca
     4081 ggagagatgg tgccgtagga ggtcgtggat ggttgatgcc aagagaggcc ctccacccgt
     4141 ggtgggcaaa tgtccaggcc tgggctggca gcccagggct gtttctgggt gctccctggc
     4201 cccagggtgg cgtctggtta ccatggctgt gtgtgtccat gtctgcaagc agttcttcaa
     4261 taaatggcct gcctccccct ccctgcctcc ctgcatctgc tagcccagtg cagtccgggg
     4321 cccccaccca gcccgtcagc ccccacctca ggtggctggc ttcccagcag aagccggacc
     4381 caggaaggga cgggttctga gttagagatc tcacataagc aaacgctgag acaggaatct
     4441 ggtcaccagc agcttgtctg ggaggtggaa ggaggcctgc aggggaggga gatcaccagt
     4501 gaaggagtgt gactgggccc gagctgccgc cacagtgggc agctggccct gcgttcctca
     4561 gggaggaccg gagagagaaa gcacacggct cacatctccc ttgagggcca gagctggggt
     4621 gtgtctacac tacccacaag agtcctgttc tgggggcagg cttctggctg tgttgaccct
     4681 tagctcccca ggtcccaggg aggataaaca gcccttggtc cccagcagat accaggctcg
     4741 tcaggtgccc gttgggcact gaaactccca tggagtattg ggggacagag agcggggctt
     4801 cctaaaggcc agttggccaa acaggcagag ccaggaagtg gctgccctgg cctcccatgg
     4861 ggcagagtca tgttggcatc aggaggcctg cggggctgag ggaacttcct gaggacctga
     4921 agtcccaggc ccaaacctcc ctcgctggga gcaaggtcac cctgtggcct ccggcctaag
     4981 gaacaaattt tttgttccat ctgtggcctc ctgttgtcgc tcagtgaatg atgggagcac
     5041 actgtgatgt ggtgtgggct gggggcatgt gggggcgggt gtccagtggc cctcagttcc
     5101 tggagctcat tcagcatctg ctcgaggctc cccagggaag gcagccccag aaggtctggc
     5161 tgcagtaggg ggtggagacc ccagggcggt ctcttcagcc cttccgttca ggatgcctca
     5221 gcgtagttga gggcctggcc acctgggctg tttctgaatg gaacaaacag catccatgtg
     5281 tgtcaacctc aacagagccc aaaaatacgc cattgaatga aacaacacat tgcaggatga
     5341 tgggctcagc ccaacctcac ttgtgtagac aatgtccccc caaattataa attttctatg
     5401 cctcagaacg tgtctataac aacatgttta aaaggtagaa aggatacaca tcccctgtag
     5461 aagagtgacc tgaactggag ttgcatttga catctgatgt cagcatgagg gtgctggtca
     5521 ccggggactt gagtacgtac tttcctttca aaagcacaat agatcaagat acaggtacac
     5581 acatagctac agatatagat ccagccacag atccagatta gatatagatg catatagaag
     5641 cctagaaaag ccaatatgga aaactctggc agtgtttgat tctgagtggc aagcatattg
     5701 gtgactgtaa ttatttcttg cagttttcta tattttttag tttcccaaac tgcaaaaaat
     5761 acccacaaca gttaccagag aaaatgctgg atgccacaag aaggaagaac ctcaaggggc
     5821 aggaggcaaa gttgtcttct tggaggaggg ggtgttagaa ataacccagg cctcctggaa
     5881 aatgacccaa atacagcctg gctctgccag ccccaggccc ttccccaccc ccagggcacc
     5941 ctttctgtcc cagtagagaa ggtgacctgg agtcaggcct tgtgtgtgct ccaagcgttt
     6001 ggagctttac cgtgggatgt ggggagccag gggtgttgtt tgctggcatt tctgtggctg
     6061 ggtctttctg gctgtggagc tgtgtttgtg ggcagtggct gagatggagg acctggggca
     6121 ggtgtctcag tgacagcgca gtatcctcca gcctcttcca gggccccacg ctactggcta
     6181 cctggcaata cagtccagca gttgctgctt ctctaggccc ctggtgcatt cagaaacctc
     6241 ctgaaggcca gcggagggta agccaggaac agatcatgtc cattgcactt cactagctga
     6301 gcgagttggt tctgcccttc tgatccttgg tccctcaggg ccaggacagg cccggtgacc
     6361 ccataatcat gcagccgtca tgctccgtca tctgaaattc acaaggaagg caaggattca
     6421 aaggaagatg tctgagatgc tgaactccag ctcagccact tgtcaactcc ttgaccttgg
     6481 acaagttact taaattaagc cttgattttt tttcatctga ataaatagag ataaaaatac
     6541 ttatttcatg gacactttca tatcagttta tttgatttaa cctcctagat ttttttttag
     6601 cacatgatag caacttctta ataaatactg ggtcccttct cctagccttc ccattgctgt
     6661 tctttttaat tccatccaca tgtcctgtgc ctgaaatatc ctgtgccatg tgcctgaaat
     6721 tccattcttc tcctggaacc cttaacaggc cacattgcta tgctccgtta gtcccctgca
     6781 tggcttaatg tattaggtaa catgaagcat gtaaggaaga actttctaac acaggtgctg
     6841 aaatgggaca ggaaatgctg atggagtgag ctctttgtca atagaagtat tcaagcaaa
//
