LOCUS       NM_014939               6230 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens trafficking protein particle complex subunit 8
            (TRAPPC8), mRNA.
ACCESSION   NM_014939 XM_375535
VERSION     NM_014939.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Zhang,Z., Bai,M., Barbosa,G.O., Chen,A., Wei,Y., Luo,S., Wang,X.,
            Wang,B., Tsukui,T., Li,H., Sheppard,D., Kornberg,T.B. and Ma,D.K.
  TITLE     Broadly conserved roles of TMEM131 family proteins in intracellular
            collagen assembly and secretory cargo trafficking
  JOURNAL   Sci Adv 6 (7), eaay7667 (2020)
   PUBMED   32095531
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6230)
  AUTHORS   Kim,J.J., Lipatova,Z. and Segev,N.
  TITLE     TRAPP Complexes in Secretion and Autophagy
  JOURNAL   Front Cell Dev Biol 4, 20 (2016)
   PUBMED   27066478
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6230)
  AUTHORS   Lamb,C.A., Nuhlen,S., Judith,D., Frith,D., Snijders,A.P.,
            Behrends,C. and Tooze,S.A.
  TITLE     TBC1D14 regulates autophagy via the TRAPP complex and ATG9 traffic
  JOURNAL   EMBO J 35 (3), 281-301 (2016)
   PUBMED   26711178
  REMARK    GeneRIF: TRAPPC8 modulates autophagy and secretory trafficking and
            is required for TBC1D14 to bind TRAPPIII.
REFERENCE   5  (bases 1 to 6230)
  AUTHORS   Scrivens,P.J., Noueihed,B., Shahrzad,N., Hul,S., Brunet,S. and
            Sacher,M.
  TITLE     C4orf41 and TTC-15 are mammalian TRAPP components with a role at an
            early stage in ER-to-Golgi trafficking
  JOURNAL   Mol Biol Cell 22 (12), 2083-2093 (2011)
   PUBMED   21525244
REFERENCE   6  (bases 1 to 6230)
  AUTHORS   Choi,C., Davey,M., Schluter,C., Pandher,P., Fang,Y., Foster,L.J.
            and Conibear,E.
  TITLE     Organization and assembly of the TRAPPII complex
  JOURNAL   Traffic 12 (6), 715-725 (2011)
   PUBMED   21453443
REFERENCE   7  (bases 1 to 6230)
  AUTHORS   Zong,M., Wu,X.G., Chan,C.W., Choi,M.Y., Chan,H.C., Tanner,J.A. and
            Yu,S.
  TITLE     The adaptor function of TRAPPC2 in mammalian TRAPPs explains
            TRAPPC2-associated SEDT and TRAPPC9-associated congenital
            intellectual disability
  JOURNAL   PLoS One 6 (8), e23350 (2011)
   PUBMED   21858081
  REMARK    GeneRIF: Data show that a disease-causing mutation of TRAPPC2,
            D47Y, failed to interact with either TRAPPC9 or TRAPPC8, suggesting
            that aspartate 47 in TRAPPC2 is at or near the site of interaction
            with TRAPPC9 or TRAPPC8.
REFERENCE   8  (bases 1 to 6230)
  AUTHORS   Ballif,B.A., Villen,J., Beausoleil,S.A., Schwartz,D. and Gygi,S.P.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
REFERENCE   9  (bases 1 to 6230)
  AUTHORS   Gavin,A.C., Bosche,M., Krause,R., Grandi,P., Marzioch,M., Bauer,A.,
            Schultz,J., Rick,J.M., Michon,A.M., Cruciat,C.M., Remor,M.,
            Hofert,C., Schelder,M., Brajenovic,M., Ruffner,H., Merino,A.,
            Klein,K., Hudak,M., Dickson,D., Rudi,T., Gnau,V., Bauch,A.,
            Bastuck,S., Huhse,B., Leutwein,C., Heurtier,M.A., Copley,R.R.,
            Edelmann,A., Querfurth,E., Rybin,V., Drewes,G., Raida,M.,
            Bouwmeester,T., Bork,P., Seraphin,B., Kuster,B., Neubauer,G. and
            Superti-Furga,G.
  TITLE     Functional organization of the yeast proteome by systematic
            analysis of protein complexes
  JOURNAL   Nature 415 (6868), 141-147 (2002)
   PUBMED   11805826
REFERENCE   10 (bases 1 to 6230)
  AUTHORS   Sacher,M., Barrowman,J., Schieltz,D., Yates,J.R. 3rd and
            Ferro-Novick,S.
  TITLE     Identification and characterization of five new subunits of TRAPP
  JOURNAL   Eur J Cell Biol 79 (2), 71-80 (2000)
   PUBMED   10727015
  REMARK    Erratum:[Eur J Cell Biol. 2000 Jul;79(7):520]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC009831.21 and AC022960.13.
            
            On May 21, 2020 this sequence version replaced NM_014939.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB023229.1, SRR14038191.1993647.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000283351.10/ ENSP00000283351.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-521               AC009831.21        54168-54688         c
            522-716             AC009831.21        42889-43083         c
            717-806             AC009831.21        29138-29227         c
            807-981             AC009831.21        27832-28006         c
            982-1135            AC009831.21        24929-25082         c
            1136-1229           AC009831.21        21227-21320         c
            1230-1486           AC009831.21        20314-20570         c
            1487-1602           AC009831.21        19863-19978         c
            1603-1753           AC009831.21        19020-19170         c
            1754-1854           AC022960.13        170710-170810       c
            1855-1960           AC022960.13        167571-167676       c
            1961-2092           AC022960.13        160520-160651       c
            2093-2317           AC022960.13        144265-144489       c
            2318-2426           AC022960.13        143215-143323       c
            2427-2621           AC022960.13        140711-140905       c
            2622-2752           AC022960.13        140157-140287       c
            2753-2827           AC022960.13        137187-137261       c
            2828-2954           AC022960.13        136634-136760       c
            2955-3109           AC022960.13        134412-134566       c
            3110-3552           AC022960.13        127325-127767       c
            3553-3700           AC022960.13        125445-125592       c
            3701-3797           AC022960.13        123634-123730       c
            3798-3866           AC022960.13        122380-122448       c
            3867-3925           AC022960.13        122231-122289       c
            3926-4099           AC022960.13        119351-119524       c
            4100-4201           AC022960.13        116501-116602       c
            4202-4347           AC022960.13        109097-109242       c
            4348-4437           AC022960.13        101869-101958       c
            4438-6230           AC022960.13        98982-100774        c
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q12.1"
     gene            1..6230
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /note="trafficking protein particle complex subunit 8"
                     /db_xref="GeneID:22878"
                     /db_xref="HGNC:HGNC:29169"
                     /db_xref="MIM:614136"
     exon            1..521
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     CDS             365..4672
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /note="general sporulation gene 1 homolog; protein TRS85
                     homolog; trafficking protein particle complex 8"
                     /codon_start=1
                     /product="trafficking protein particle complex subunit 8"
                     /protein_id="NP_055754.3"
                     /db_xref="CCDS:CCDS11901.1"
                     /db_xref="GeneID:22878"
                     /db_xref="HGNC:HGNC:29169"
                     /db_xref="MIM:614136"
                     /translation="MAQCVQSVQELIPDSFVPCVAALCSDEAERLTRLNHLSFAELLK
                     PFSRLTSEVHMRDPNNQLHVIKNLKIAVSNIVTQPPQPGAIRKLLNDVVSGSQPAEGL
                     VANVITAGDYDLNISATTPWFESYRETFLQSMPALDHEFLNHYLACMLVASSSEAEPV
                     EQFSKLSQEQHRIQHNSDYSYPKWFIPNTLKYYVLLHDVSAGDEQRAESIYEEMKQKY
                     GTQGCYLLKINSRTSNRASDEQIPDPWSQYLQKNSIQNQESYEDGPCTITSNKNSDNN
                     LLSLDGLDNEVKDGLPNNFRAHPLQLEQSSDPSNSIDGPDHLRSASSLHETKKGNTGI
                     IHGACLTLTDHDRIRQFIQEFTFRGLLPHIEKTIRQLNDQLISRKGLSRSLFSATKKW
                     FSGSKVPEKSINDLKNTSGLLYPPEAPELQIRKMADLCFLVQHYDLAYSCYHTAKKDF
                     LNDQAMLYAAGALEMAAVSAFLQPGAPRPYPAHYMDTAIQTYRDICKNMVLAERCVLL
                     SAELLKSQSKYSEAAALLIRLTSEDSDLRSALLLEQAAHCFINMKSPMVRKYAFHMIL
                     AGHRFSKAGQKKHALRCYCQAMQVYKGKGWSLAEDHINFTIGRQSYTLRQLDNAVSAF
                     RHILINESKQSAAQQGAFLREYLYVYKNVSQLSPDGPLPQLPLPYINSSATRVFFGHD
                     RRPADGEKQAATHVSLDQEYDSESSQQWRELEEQVVSVVNKGVIPSNFHPTQYCLNSY
                     SDNSRFPLAVVEEPITVEVAFRNPLKVLLLLTDLSLLWKFHPKDFSGKDNEEVKQLVT
                     SEPEMIGAEVISEFLINGEESKVARLKLFPHHIGELHILGVVYNLGTIQGSMTVDGIG
                     ALPGCHTGKYSLSMSVRGKQDLEIQGPRLNNTKEEKTSVKYGPDRRLDPIITEEMPLL
                     EVFFIHFPTGLLCGEIRKAYVEFVNVSKCPLTGLKVVSKRPEFFTFGGNTAVLTPLSP
                     SASENCSAYKTVVTDATSVCTALISSASSVDFGIGTGSQPEVIPVPLPDTVLLPGASV
                     QLPMWLRGPDEEGVHEINFLFYYESVKKQPKIRHRILRHTAIICTSRSLNVRATVCRS
                     NSLENEEGRGGNMLVFVDVENTNTSEAGVKEFHIVQVSSSSKHWKLQKSVNLSENKDT
                     KLASREKGKFCFKAIRCEKEEAATQSSEKYTFADIIFGNEQIISSASPCADFFYRSLS
                     SELKKPQAHLPVHTEKQSTEDAVRLIQKCSEVDLNIVILWKAYVVEDSKQLILEGQHH
                     VILRTIGKEAFSYPQKQEPPEMELLKFFRPENITVSSRPSVEQLSSLIKTSLHYPESF
                     NHPFHQKSLCLVPVTLLLSNCSKADVDVIVDLRHKTTSPEALEIHGSFTWLGQTQYKL
                     QLKSQEIHSLQLKACFVHTGVYNLGTPRVFAKLSDQVTVFETSQQNSMPALIIISNV"
     exon            522..716
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            717..806
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            807..981
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1135
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1229
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            1230..1486
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            1487..1602
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            1603..1753
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            1754..1854
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            1855..1960
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            1961..2092
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            2093..2317
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            2318..2426
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            2427..2621
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            2622..2752
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            2753..2827
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            2828..2954
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            2955..3109
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            3110..3552
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            3553..3700
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            3701..3797
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            3798..3866
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            3867..3925
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            3926..4099
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            4100..4201
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            4202..4347
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            4348..4437
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     exon            4438..6230
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5464..5469
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /note="hexamer: AATAAA"
     polyA_site      5487
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /note="major polyA site"
     regulatory      6206..6211
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
                     /note="hexamer: AATAAA"
     polyA_site      6230
                     /gene="TRAPPC8"
                     /gene_synonym="GSG1; HsT2706; KIAA1012; TRS85"
ORIGIN      
        1 gagtagtact gccggttggt cagggttcgg cctcaacatg gcggttccgg ccgggcagtg
       61 accaaggttt tgccgtccga aggactaagt ggtgactgtc ggcgtctcca cctatcgggg
      121 tggaatgcga gcacgcgggg acgagcgctg agaagcggcg gtgacgggaa ggggggggtc
      181 agtggattgt gccccagaaa ccgaagcggc ggcgtctgtt ccttcttgtt tctaggctcc
      241 agtgcgttcg gggccccgcc cggccgggcc aggccggcgg gcggcggcgg tagctgctgc
      301 agccgcagga taacctcgca gggtgggccg gagggcgggc gccgccgctg cctgtgctgc
      361 ggcgatggcc cagtgtgtac aatcagtgca ggagctaatc ccggactcct tcgtcccctg
      421 tgtcgctgcg ctgtgcagcg acgaagccga gcggctcact cgtctcaatc acctcagctt
      481 cgcggagctg cttaagccct tctcccgcct cacttccgag gttcacatga gagatcctaa
      541 taatcaactt cacgtaatta aaaatttgaa gatagcagta agcaacattg tcacccagcc
      601 acctcagcct ggagccatcc ggaagctttt gaatgatgtt gtttctggca gtcagcctgc
      661 agaaggatta gtagctaatg tgattacagc aggagattat gaccttaaca tcagtgccac
      721 tactccatgg tttgagtctt acagagaaac ctttcttcag tcgatgccag cattggatca
      781 tgaatttctg aaccactatt tagcatgtat gttggtagcg tcatctagtg aagctgaacc
      841 tgtggaacag ttttcaaagt tgtcacaaga acagcatcga attcagcaca acagtgatta
      901 ttcctacccc aagtggttta taccaaatac acttaaatac tatgtacttt tacatgatgt
      961 aagtgcagga gatgaacaga gagctgaatc aatttatgaa gaaatgaaac agaaatatgg
     1021 aactcagggt tgctatttac ttaaaattaa ttctcgaaca tctaatcgag catcagatga
     1081 acagatacca gatccttgga gtcagtatct ccagaaaaat agtattcaaa accaggaatc
     1141 atatgaagat ggcccttgta ctataacttc aaataagaat tctgataata acttgctttc
     1201 attggatgga ttagataacg aagtcaaaga tggcttacca aataacttta gagctcaccc
     1261 acttcagttg gagcaatcca gtgacccttc taacagtatt gatggcccag atcatctaag
     1321 atctgcttca tcgttacatg aaacaaagaa aggaaatact ggaataattc atggtgcatg
     1381 tttaacactt actgatcatg atagaattcg acagtttata caagagttca catttcgggg
     1441 ccttttgcca catatagaga aaacaattag gcaattaaac gatcagctaa tatcaagaaa
     1501 aggtttgagt cgatctctat tttctgcaac taaaaaatgg tttagtggca gtaaagttcc
     1561 agaaaagagc attaatgacc tgaaaaatac atctggcttg ctgtatccgc cggaagcacc
     1621 agaacttcaa atcaggaaaa tggctgactt atgttttttg gtgcagcatt atgatttggc
     1681 ttacagttgc tatcatactg caaagaaaga ttttcttaat gatcaagcaa tgctttatgc
     1741 agctggtgcc ttggaaatgg cagcagtgtc tgcttttctt caaccaggag cacctaggcc
     1801 atatcctgct cattacatgg atacagcaat tcagacatac agagatatct gcaagaatat
     1861 ggtgttggct gaaagatgtg tgttgcttag tgctgaactt ttaaaaagcc aaagcaaata
     1921 ttcagaggct gcagctctcc taatacggtt gaccagtgag gattctgatc ttcgaagtgc
     1981 acttcttttg gaacaggcag cacattgctt tataaacatg aaaagtccca tggttagaaa
     2041 atatgcattt catatgatat tggcaggcca tcgatttagt aaagcagggc agaaaaagca
     2101 tgctttacgc tgttattgtc aagccatgca agtttacaaa ggaaaaggct ggtctcttgc
     2161 agaggatcac attaatttca ctattgggcg ccagtcctat actcttagac agctggataa
     2221 tgctgtgtct gcttttaggc atattctaat taatgaaagt aaacaatctg ctgctcaaca
     2281 gggggctttc ctcagagaat atctttatgt ttacaagaat gtaagtcagc tgtcaccaga
     2341 tggtcctttg ccacagcttc ctttaccgta tattaacagt tcagcaacac gggttttttt
     2401 tggccatgac agacgaccag cggatggtga aaaacaagca gctactcatg taagtcttga
     2461 tcaagaatat gattctgaat cctctcagca gtggcgagaa cttgaggaac aagttgtttc
     2521 tgtggttaac aaaggagtaa ttccatccaa ttttcatccc acacaatact gtttgaacag
     2581 ttactcagat aattcaagat ttccacttgc agttgtagaa gaaccaatta cagtggaagt
     2641 ggcttttaga aaccctttga aagttctact tttgttgact gatttgtcat tgctttggaa
     2701 gtttcatcct aaagatttca gtggaaagga taatgaagaa gttaaacaac tagttacaag
     2761 tgaacctgaa atgattggag ctgaagttat ttcagagttc ttaattaatg gcgaagaatc
     2821 aaaagtggca agactaaagc tctttcccca tcacataggg gagctgcata ttctgggagt
     2881 tgtttataat cttggcacta ttcagggctc tatgacagta gatggcattg gtgctcttcc
     2941 cggatgtcac acaggaaaat attccttgag tatgtcagtc cgagggaagc aggatttaga
     3001 aattcaaggt cctcgactta acaacacaaa agaagagaaa acatctgtta aatatggccc
     3061 tgatcgacgt ttagatccca taatcacaga agaaatgcca ctgttggagg tgttctttat
     3121 acattttcct acagggcttc tctgtggaga aatccgaaaa gcatatgtag aatttgtcaa
     3181 tgtcagcaaa tgtccactta ctggattgaa ggttgtttct aaacgtccag agttctttac
     3241 tttcggtggt aatactgctg ttctaacacc actaagtccc tcagcttctg agaattgtag
     3301 tgcttacaag actgttgtga cagatgctac ctctgtgtgt acagcactca tatcatcagc
     3361 ttcttctgta gactttggca ttggcacagg aagtcaacca gaggtgattc ctgttcccct
     3421 tcctgacact gttcttctac ccggagcctc agtgcagctg ccaatgtggt tacgtgggcc
     3481 tgatgaagaa ggtgtccatg aaattaactt tttgttttac tatgaaagtg tcaaaaagca
     3541 gccaaaaata cggcacagaa tattaagaca cactgcaatt atttgtacca gtcggtcttt
     3601 aaatgtacgg gccactgtct gcagaagtaa ttctcttgaa aatgaagaag gcagaggagg
     3661 caatatgcta gtctttgtgg atgtggaaaa taccaatact agtgaagcag gcgttaagga
     3721 attccacata gtgcaagtat caagtagtag caaacactgg aagttacaga aatctgtaaa
     3781 tctttctgaa aacaaagata ccaaacttgc cagtagggag aagggaaagt tttgctttaa
     3841 ggcaataaga tgtgagaaag aagaagcggc cacacagtcc tctgaaaaat atacctttgc
     3901 agatatcatc tttggaaatg aacagataat aagttcagca agcccatgtg cagacttctt
     3961 ttatcgaagt ttatcttctg aattgaaaaa accacaagct cacttgcctg tgcatacaga
     4021 aaaacagtca acagaggatg ctgtgagatt gattcaaaaa tgcagtgagg tagatttgaa
     4081 tattgtcata ttatggaagg catacgttgt ggaagacagt aaacagctta ttttggaagg
     4141 tcaacatcat gttattcttc gcactatagg aaaagaagcc ttttcatatc ctcagaaaca
     4201 ggagccacca gaaatggaac tattgaaatt tttcaggcca gaaaacatta cagtttcctc
     4261 aaggccatca gtagagcagc tttctagtct cattaaaacg agtcttcact acccagaatc
     4321 atttaatcat ccatttcatc aaaaaagcct ttgtttagta ccagtcactc ttttactttc
     4381 caattgttct aaggctgatg tagatgtcat agttgatctt cggcataaaa caacaagtcc
     4441 agaagcactg gaaatccatg gatcattcac atggcttgga caaacacagt ataaacttca
     4501 acttaaaagc caggagattc acagtctgca gctgaaagca tgctttgttc atacaggtgt
     4561 ttataacctt ggaactccta gggtatttgc caagttatcg gaccaagtta cagtgtttga
     4621 aacaagtcag cagaattcca tgcctgccct gatcatcatc agtaatgtgt gacaacttgg
     4681 aaatttgtac tgaaatccac aataatcagt ttttgctgga tgggttttac agcagtattt
     4741 gatataccta acttgttatg gaggttgatt gatatctgat ccctgcaaaa tactttgact
     4801 tgtcattttg ttgatgatgc aaagcacgtt ggactgagaa tacttaacat tctttttctg
     4861 tatttcttta aaccctgaga ataatttaca tgctcataat acaggatatc agcatatttg
     4921 tgcaccttat taagccccat cttaagaaaa cacaaagtct aagtctgctg ttacaacttg
     4981 tcaatggtat acgaatatta ggagatgatt ctgagaaagg aaaggccttg ttggcagtac
     5041 tcctgttaag ccattagtct ctaaattcca gctttactgt gaagttctat agagtgttaa
     5101 atacaaattt tcctgtcttg cttcacacag ttccttaaaa tcagttttga actttggtca
     5161 tagagtcttc atatttcagt atttggtggt ccctatgact tatacataac tttgtaaaaa
     5221 gaaaaaaaaa ttttttctga tgctttgaat atagttttga aaggagtttt gacttttttc
     5281 ccctcattca tctcagtata gagtgcgcta tttcacaata cgatttttgt cattaaaatt
     5341 accatattct ttattatata acgttaacta ttgagttgat ctgtttaaaa tataaatctc
     5401 aagttaatta aaaataagct tttcaaaaat gtattatatt tataacaaat atactgtaaa
     5461 tagaataaag acatgctatt cactgtatag cgtattagta tctgttgatt agaaagtctg
     5521 gtttcaaaat atttttttat tcagagaatg ggcatttgga cataagaaat ttcaattcag
     5581 ccctgctgca gatagagaaa tgggagcaag gttgtttcaa tgaatatctc ttgtcattgg
     5641 agaaggaggg aagacagggt gtaggagtgg gtggcaggga gtacaagagc cagggtcagt
     5701 caagtctgga gcagatgggg tccttgtgcc attgacagct accatggttt gttccagtct
     5761 caatctcggc actaatcgga gcagactgca ccaaagtggg gagagatcaa aagaccccgt
     5821 tttcagactg aagattccaa acctgtactt ggaacaactc ttcttgagag atgggtggct
     5881 agtctgtgtg cctctctgca actgctgctt atgcttctgg atcttaagtg ggtctctggc
     5941 ttttcagctc cttccactct gtgtcttagc aggtgtggga caagttggag aggaaggtca
     6001 cattctaagg acactcattt ccttcttgag agtttagggc catgggctta taaaaagtct
     6061 gaggaggaaa gacaagaatg aggaggcata atcacagtgg tatctcatat ttaaaccact
     6121 cagctgaata ttaaattgaa ccgattgtta aacagacact aataaaaaaa ttaaactata
     6181 taaacctaca actgaatata ttaaaaataa aggtaataaa gcactcaaaa
//
