LOCUS       NM_014408               1282 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens trafficking protein particle complex subunit 3
            (TRAPPC3), transcript variant 1, mRNA.
ACCESSION   NM_014408
VERSION     NM_014408.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1282)
  AUTHORS   Wang,Y., Anderson,E.P. and Tatakis,D.N.
  TITLE     Whole transcriptome analysis of smoker palatal mucosa identifies
            multiple downregulated innate immunity genes
  JOURNAL   J Periodontol 91 (6), 756-766 (2020)
   PUBMED   31682009
  REMARK    GeneRIF: TRAPPC3 expression in healthy human palatal mucosa is
            strongly negatively correlated with serum cotinine levels.
REFERENCE   4  (bases 1 to 1282)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1282)
  AUTHORS   Kummel,D., Muller,J.J., Roske,Y., Henke,N. and Heinemann,U.
  TITLE     Structure of the Bet3-Tpc6B core of TRAPP: two Tpc6 paralogs form
            trimeric complexes with Bet3 and Mum2
  JOURNAL   J Mol Biol 361 (1), 22-32 (2006)
   PUBMED   16828797
  REMARK    GeneRIF: The crystal structure of human Bet3-Tpc6B heterodimer
            presented here represents a core sub-complex in the assembly of
            TRAPP.
REFERENCE   6  (bases 1 to 1282)
  AUTHORS   Kim,M.S., Yi,M.J., Lee,K.H., Wagner,J., Munger,C., Kim,Y.G.,
            Whiteway,M., Cygler,M., Oh,B.H. and Sacher,M.
  TITLE     Biochemical and crystallographic studies reveal a specific
            interaction between TRAPP subunits Trs33p and Bet3p
  JOURNAL   Traffic 6 (12), 1183-1195 (2005)
   PUBMED   16262728
REFERENCE   7  (bases 1 to 1282)
  AUTHORS   Loh,E., Peter,F., Subramaniam,V.N. and Hong,W.
  TITLE     Mammalian Bet3 functions as a cytosolic factor participating in
            transport from the ER to the Golgi apparatus
  JOURNAL   J Cell Sci 118 (Pt 6), 1209-1222 (2005)
   PUBMED   15728249
  REMARK    GeneRIF: Bet3 functions as a cytosolic factor participating in
            transport from the ER to the Golgi apparatus
REFERENCE   8  (bases 1 to 1282)
  AUTHORS   Turnbull,A.P., Kummel,D., Prinz,B., Holz,C., Schultchen,J.,
            Lang,C., Niesen,F.H., Hofmann,K.P., Delbruck,H., Behlke,J.,
            Muller,E.C., Jarosch,E., Sommer,T. and Heinemann,U.
  TITLE     Structure of palmitoylated BET3: insights into TRAPP complex
            assembly and membrane localization
  JOURNAL   EMBO J 24 (5), 875-884 (2005)
   PUBMED   15692564
  REMARK    GeneRIF: crystal structure
REFERENCE   9  (bases 1 to 1282)
  AUTHORS   Kim,Y.G., Sohn,E.J., Seo,J., Lee,K.J., Lee,H.S., Hwang,I.,
            Whiteway,M., Sacher,M. and Oh,B.H.
  TITLE     Crystal structure of bet3 reveals a novel mechanism for Golgi
            localization of tethering factor TRAPP
  JOURNAL   Nat Struct Mol Biol 12 (1), 38-45 (2005)
   PUBMED   15608655
REFERENCE   10 (bases 1 to 1282)
  AUTHORS   Sacher,M., Jiang,Y., Barrowman,J., Scarpa,A., Burston,J., Zhang,L.,
            Schieltz,D., Yates,J.R. 3rd, Abeliovich,H. and Ferro-Novick,S.
  TITLE     TRAPP, a highly conserved novel complex on the cis-Golgi that
            mediates vesicle docking and fusion
  JOURNAL   EMBO J 17 (9), 2494-2503 (1998)
   PUBMED   9564032
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG704657.1 and BC007662.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_014408.4.
            
            Summary: This gene encodes a component of the trafficking protein
            particle complex, which tethers transport vesicles to the cis-Golgi
            membrane. The encoded protein participates in the regulation of
            transport from the endoplasmic reticulum to the Golgi apparatus.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Aug 2012].
            
            Transcript Variant: This variant (1) encodes isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189652.30649.1,
                                           SRR5189655.48863.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142853, SAMEA2148874
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373166.8/ ENSP00000362261.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                BG704657.1         34-53
            21-1282             BC007662.2         1-1262
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p34.3"
     gene            1..1282
                     /gene="TRAPPC3"
                     /gene_synonym="BET3"
                     /note="trafficking protein particle complex subunit 3"
                     /db_xref="GeneID:27095"
                     /db_xref="HGNC:HGNC:19942"
                     /db_xref="MIM:610955"
     exon            1..150
                     /gene="TRAPPC3"
                     /gene_synonym="BET3"
                     /inference="alignment:Splign:2.1.0"
     CDS             109..651
                     /gene="TRAPPC3"
                     /gene_synonym="BET3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     trafficking protein particle complex 3"
                     /codon_start=1
                     /product="trafficking protein particle complex subunit 3
                     isoform 1"
                     /protein_id="NP_055223.1"
                     /db_xref="CCDS:CCDS404.1"
                     /db_xref="GeneID:27095"
                     /db_xref="HGNC:HGNC:19942"
                     /db_xref="MIM:610955"
                     /translation="MSRQANRGTESKKMSSELFTLTYGALVTQLCKDYENDEDVNKQL
                     DKMGFNIGVRLIEDFLARSNVGRCHDFRETADVIAKVAFKMYLGITPSITNWSPAGDE
                     FSLILENNPLVDFVELPDNHSSLIYSNLLCGVLRGALEMVQMAVEAKFVQDTLKGDGV
                     TEIRMRFIRRIEDNLPAGEE"
     exon            151..248
                     /gene="TRAPPC3"
                     /gene_synonym="BET3"
                     /inference="alignment:Splign:2.1.0"
     exon            249..348
                     /gene="TRAPPC3"
                     /gene_synonym="BET3"
                     /inference="alignment:Splign:2.1.0"
     exon            349..531
                     /gene="TRAPPC3"
                     /gene_synonym="BET3"
                     /inference="alignment:Splign:2.1.0"
     exon            532..1282
                     /gene="TRAPPC3"
                     /gene_synonym="BET3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1252..1257
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRAPPC3"
                     /gene_synonym="BET3"
                     /note="hexamer: AATAAA"
     polyA_site      1282
                     /gene="TRAPPC3"
                     /gene_synonym="BET3"
                     /note="major polyA site"
ORIGIN      
        1 agtgccggtc ggtcttgtgg gctgaggggc agcggcttag gctccggcgt ctgcaggggt
       61 cgccgagcta acccgtggct aggcgagtgg ggcggggcgg ccggcaccat gtcgaggcag
      121 gcgaaccgtg gcaccgagag caagaaaatg agctctgagc tcttcaccct gacctatggt
      181 gccctggtca cccagctatg taaggactat gaaaatgatg aagatgtgaa taaacagctg
      241 gacaaaatgg gctttaacat tggagtccgg ctgattgaag atttcttggc tcggtcaaat
      301 gttgggaggt gccatgactt tcgggaaact gcggatgtca ttgccaaggt ggcgttcaag
      361 atgtacttgg gcatcactcc aagcattact aattggagcc cagctggtga tgaattctcc
      421 ctcattttgg aaaataaccc cttggtggac tttgtggaac ttcctgataa ccactcatcc
      481 cttatttatt ccaatctctt gtgtggggtg ttgcggggag ctttggagat ggtccagatg
      541 gctgtggagg ccaagtttgt ccaggacacc ctgaaaggag acggtgtgac agaaatccgg
      601 atgagattca tcaggcggat tgaggacaat cttccagctg gagaggaata accatcccta
      661 caactcgagg atagccatca ggagcactgt tggaatcagc aggcctctgt gctccctctg
      721 ccctccagaa ctcagtgact cttgaacatg gatgttatat attcttataa cctgtttcca
      781 ttctccattc aaataaagag cagactgcga tatagtccat ttaccccatg tgtgcacatt
      841 caggagcgac agtctctgcc cccattccct tgagaggggc tggatgtaat cacctttggt
      901 tggactagaa agagctcaaa ccattttaca ttcctgtttg aatttttcca aagcaaaact
      961 cactttgacc ccattaagag gcaagcctgg cacatctatc cctgggcctt tagaaagcca
     1021 tttgcctcaa atggctatag ggttgtgggg tggagggagg aagggctggg agggagtggg
     1081 gaggaattgc tagctgtagt gtgacacatt gtagtgtttg ccaggaaagg agccagtcat
     1141 gccggaaaca ctgacttctg ggaagccacc caggtctcat tcctccctgc tgttggaggc
     1201 aacatctcct ctttttacag agggtacatc cttttttctt acaaattctt caataaagac
     1261 acattcttga gtgaaatccc aa
//
