LOCUS       NM_016030               2499 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens trafficking protein particle complex subunit 12
            (TRAPPC12), transcript variant 1, mRNA.
ACCESSION   NM_016030
VERSION     NM_016030.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Milev,M.P., Grout,M.E., Saint-Dic,D., Cheng,Y.H., Glass,I.A.,
            Hale,C.J., Hanna,D.S., Dorschner,M.O., Prematilake,K., Shaag,A.,
            Elpeleg,O., Sacher,M., Doherty,D. and Edvardson,S.
  TITLE     Mutations in TRAPPC12 Manifest in Progressive Childhood
            Encephalopathy and Golgi Dysfunction
  JOURNAL   Am J Hum Genet 101 (2), 291-299 (2017)
   PUBMED   28777934
  REMARK    GeneRIF: The authors report that variants in TRAPPC12 result in
            progressive childhood encephalopathy.  Fibroblasts derived from
            three affected individuals showed a fragmented Golgi that could be
            rescued by expression of wild-type TRAPPC12.
REFERENCE   3  (bases 1 to 2499)
  AUTHORS   Zhao,S., Li,C.M., Luo,X.M., Siu,G.K., Gan,W.J., Zhang,L., Wu,W.K.,
            Chan,H.C. and Yu,S.
  TITLE     Mammalian TRAPPIII Complex positively modulates the recruitment of
            Sec13/31 onto COPII vesicles
  JOURNAL   Sci Rep 7, 43207 (2017)
   PUBMED   28240221
  REMARK    GeneRIF: TRAPPC12, but not TRAPPC8, bound to Sec13/Sec31A tetramer
            but each Sec protein alone could not interact with TRAPPC12.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2499)
  AUTHORS   Kim,J.J., Lipatova,Z. and Segev,N.
  TITLE     TRAPP Complexes in Secretion and Autophagy
  JOURNAL   Front Cell Dev Biol 4, 20 (2016)
   PUBMED   27066478
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2499)
  AUTHORS   Milev,M.P., Hasaj,B., Saint-Dic,D., Snounou,S., Zhao,Q. and
            Sacher,M.
  TITLE     TRAMM/TrappC12 plays a role in chromosome congression, kinetochore
            stability, and CENP-E recruitment
  JOURNAL   J Cell Biol 209 (2), 221-234 (2015)
   PUBMED   25918224
REFERENCE   6  (bases 1 to 2499)
  AUTHORS   Comuzzie,A.G., Cole,S.A., Laston,S.L., Voruganti,V.S., Haack,K.,
            Gibbs,R.A. and Butte,N.F.
  TITLE     Novel genetic loci identified for the pathophysiology of childhood
            obesity in the Hispanic population
  JOURNAL   PLoS One 7 (12), e51954 (2012)
   PUBMED   23251661
REFERENCE   7  (bases 1 to 2499)
  AUTHORS   Scrivens,P.J., Noueihed,B., Shahrzad,N., Hul,S., Brunet,S. and
            Sacher,M.
  TITLE     C4orf41 and TTC-15 are mammalian TRAPP components with a role at an
            early stage in ER-to-Golgi trafficking
  JOURNAL   Mol Biol Cell 22 (12), 2083-2093 (2011)
   PUBMED   21525244
REFERENCE   8  (bases 1 to 2499)
  AUTHORS   Behrends,C., Sowa,M.E., Gygi,S.P. and Harper,J.W.
  TITLE     Network organization of the human autophagy system
  JOURNAL   Nature 466 (7302), 68-76 (2010)
   PUBMED   20562859
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA031172.1, BC014164.2, BC017475.1 and AF151845.1.
            
            On Nov 23, 2018 this sequence version replaced NM_016030.5.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.710119.1,
                                           SRR1163658.177467.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000324266.10/ ENSP00000324318.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-104               DA031172.1         21-124
            105-1884            BC014164.2         1-1780
            1885-2028           BC017475.1         902-1045
            2029-2499           AF151845.1         777-1247
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p25.3"
     gene            1..2499
                     /gene="TRAPPC12"
                     /gene_synonym="CGI-87; PEBAS; TTC-15; TTC15"
                     /note="trafficking protein particle complex subunit 12"
                     /db_xref="GeneID:51112"
                     /db_xref="HGNC:HGNC:24284"
                     /db_xref="MIM:614139"
     exon            1..183
                     /gene="TRAPPC12"
                     /gene_synonym="CGI-87; PEBAS; TTC-15; TTC15"
                     /inference="alignment:Splign:2.1.0"
     exon            184..1234
                     /gene="TRAPPC12"
                     /gene_synonym="CGI-87; PEBAS; TTC-15; TTC15"
                     /inference="alignment:Splign:2.1.0"
     CDS             188..2395
                     /gene="TRAPPC12"
                     /gene_synonym="CGI-87; PEBAS; TTC-15; TTC15"
                     /note="tetratricopeptide repeat protein 15; TPR repeat
                     protein 15; tetratricopeptide repeat domain 15;
                     trafficking of membranes and mitosis; trafficking protein
                     particle complex 12"
                     /codon_start=1
                     /product="trafficking protein particle complex subunit 12"
                     /protein_id="NP_057114.5"
                     /db_xref="CCDS:CCDS1652.1"
                     /db_xref="GeneID:51112"
                     /db_xref="HGNC:HGNC:24284"
                     /db_xref="MIM:614139"
                     /translation="MEDAGGGEETPAPEAPHPPQLAPPEEQGLLFQEETIDLGGDEFG
                     SEENETASEGSSPLADKLNEHMMESVLISDSPNSEGDAGDLGRVRDEAEPGGEGDPGP
                     EPAGTPSPSGEADGDCAPEDAAPSSGGAPRQDAAREVPGSEAARPEQEPPVAEPVPVC
                     TIFSQRAPPASGDGFEPQMVKSPSFGGASEASARTPPQVVQPSPSLSTFFGDTAASHS
                     LASDFFDSFTTSAFISVSNPGAGSPAPASPPPLAVPGTEGRPEPVAMRGPQAAAPPAS
                     PEPFAHIQAVFAGSDDPFATALSMSEMDRRNDAWLPGEATRGVLRAVATQQRGAVFVD
                     KENLTMPGLRFDNIQGDAVKDLMLRFLGEKAAAKRQVLNADSVEQSFVGLKQLISCRN
                     WRAAVDLCGRLLTAHGQGYGKSGLLTSHTTDSLQLWFVRLALLVKLGLFQNAEMEFEP
                     FGNLDQPDLYYEYYPHVYPGRRGSMVPFSMRILHAELQQYLGNPQESLDRLHKVKTVC
                     SKILANLEQGLAEDGGMSSVTQEGRQASIRLWRSRLGRVMYSMANCLLLMKDYVLAVE
                     AYHSVIKYYPEQEPQLLSGIGRISLQIGDIKTAEKYFQDVEKVTQKLDGLQGKIMVLM
                     NSAFLHLGQNNFAEAHRFFTEILRMDPRNAVANNNAAVCLLYLGKLKDSLRQLEAMVQ
                     QDPRHYLHESVLFNLTTMYELESSRSMQKKQALLEAVAGKEGDSFNTQCLKLA"
     exon            1235..1351
                     /gene="TRAPPC12"
                     /gene_synonym="CGI-87; PEBAS; TTC-15; TTC15"
                     /inference="alignment:Splign:2.1.0"
     exon            1352..1465
                     /gene="TRAPPC12"
                     /gene_synonym="CGI-87; PEBAS; TTC-15; TTC15"
                     /inference="alignment:Splign:2.1.0"
     exon            1466..1604
                     /gene="TRAPPC12"
                     /gene_synonym="CGI-87; PEBAS; TTC-15; TTC15"
                     /inference="alignment:Splign:2.1.0"
     exon            1605..1717
                     /gene="TRAPPC12"
                     /gene_synonym="CGI-87; PEBAS; TTC-15; TTC15"
                     /inference="alignment:Splign:2.1.0"
     exon            1718..1790
                     /gene="TRAPPC12"
                     /gene_synonym="CGI-87; PEBAS; TTC-15; TTC15"
                     /inference="alignment:Splign:2.1.0"
     exon            1791..1864
                     /gene="TRAPPC12"
                     /gene_synonym="CGI-87; PEBAS; TTC-15; TTC15"
                     /inference="alignment:Splign:2.1.0"
     exon            1865..1963
                     /gene="TRAPPC12"
                     /gene_synonym="CGI-87; PEBAS; TTC-15; TTC15"
                     /inference="alignment:Splign:2.1.0"
     exon            1964..2064
                     /gene="TRAPPC12"
                     /gene_synonym="CGI-87; PEBAS; TTC-15; TTC15"
                     /inference="alignment:Splign:2.1.0"
     exon            2065..2152
                     /gene="TRAPPC12"
                     /gene_synonym="CGI-87; PEBAS; TTC-15; TTC15"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2499
                     /gene="TRAPPC12"
                     /gene_synonym="CGI-87; PEBAS; TTC-15; TTC15"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2482..2487
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRAPPC12"
                     /gene_synonym="CGI-87; PEBAS; TTC-15; TTC15"
                     /note="hexamer: AATAAA"
     polyA_site      2499
                     /gene="TRAPPC12"
                     /gene_synonym="CGI-87; PEBAS; TTC-15; TTC15"
                     /note="major polyA site"
ORIGIN      
        1 gcctcgggtt cctgcatcgg gcctgagcag aactaggcgc gccgcggccc ggcacgcgca
       61 ggcgtacaga gctccccggg ggtgccagtt cctctccgat tcccggcttc tgtcaccttc
      121 ctcacggacc ttggtcacgg ccgcaggtga ccccttagcc cagctccagt gggcgggtgg
      181 cagggtcatg gaggacgctg gcggcggcga ggagaccccg gccccggagg ccccgcaccc
      241 ccctcagctc gcgcctccgg aggagcaggg gttgctcttc caggaggaaa ccatcgatct
      301 tggcggagat gagtttggat ccgaagagaa cgagaccgca tcggaaggct cgagtcctct
      361 cgcggacaag ctgaacgaac acatgatgga gagcgtcctc atctctgact cccccaacag
      421 cgagggcgac gcgggcgacc tgggccgagt gcgggacgaa gctgagcccg gaggggaagg
      481 cgacccaggc ccggagcccg cgggcacccc gagtcccagc ggcgaggccg acggcgactg
      541 tgcccccgag gacgcggcac ccagtagcgg aggggccccg aggcaggacg cggcccgcga
      601 ggtcccaggc agcgaagccg cgcgcccgga gcaggagcct cccgttgcgg agccggtccc
      661 ggtgtgcacc atcttcagcc agcgcgcgcc cccagcctcc ggggacggct tcgagccgca
      721 gatggtgaag tcgcccagct tcggtggcgc cagcgaggcc tcggccagga caccgcccca
      781 ggtcgtgcag cccagcccca gcctcagcac gttcttcgga gacacggccg ccagccactc
      841 cttggcctcg gacttcttcg actcctttac tacctccgcc ttcatttccg tcagcaatcc
      901 cggcgcgggc tccccggccc ccgccagccc gcctcccctc gctgtgcccg ggaccgaggg
      961 gcgccccgaa cccgtggcca tgcgagggcc ccaggcagct gcgcccccgg cgtcgccaga
     1021 gcctttcgcg cacatccagg cagtgtttgc agggagtgac gacccctttg ccaccgccct
     1081 gagcatgagc gagatggacc ggaggaacga cgcctggctt cccggcgagg ctacgcgtgg
     1141 agtcctgcgg gccgtggcca cccagcagcg cggcgccgtg ttcgtggaca aggagaacct
     1201 caccatgccg ggcctcaggt tcgacaacat ccagggagat gcagttaaag acttgatgct
     1261 tcgctttctg ggtgaaaaag ctgcagcaaa gagacaagtc ctaaatgccg actcagtgga
     1321 acaatctttt gttggattga aacagctaat cagctgcaga aactggaggg cagcagtgga
     1381 cctgtgcgga cgtctcctca cagcccacgg ccagggctac ggcaagagcg ggctgctcac
     1441 cagccacacg acagattcac tgcagctctg gtttgtcagg ctggcactac tagtgaagtt
     1501 gggccttttc cagaatgctg agatggaatt tgaacccttc ggaaatcttg atcagccaga
     1561 tctttattac gagtactacc cgcacgtgta ccctgggcgc aggggctcca tggtcccctt
     1621 ctcgatgcgc atcttgcacg cggagcttca gcagtacctg gggaacccac aggagtcgct
     1681 ggatagactg cacaaggtga agactgtctg cagcaagatc ctggccaatt tggagcaagg
     1741 cttagcagaa gacggcggca tgagcagcgt gactcaggag ggcagacaag cctctatccg
     1801 gctgtggagg tcacgtctgg gccgggtgat gtactccatg gcaaactgtc tgctcctgat
     1861 gaaggattat gtgctggccg tggaggcgta tcattcggtt atcaagtatt acccagagca
     1921 agagccccag ctgctcagcg gcatcggccg gatttccctg cagattggag acataaaaac
     1981 agctgaaaag tattttcaag acgttgagaa agtaacacag aaattagatg gactacaggg
     2041 taaaatcatg gttttgatga acagcgcgtt ccttcacctc gggcagaata actttgcaga
     2101 agcccacagg ttcttcacag agatcttaag gatggatcca agaaacgcag tggccaacaa
     2161 caacgctgcc gtgtgtctgc tctacctggg caagctcaag gactccctgc ggcagctgga
     2221 ggccatggtc cagcaggacc ccaggcacta cctgcacgag agcgtgctct tcaacctgac
     2281 caccatgtac gagctggagt cctcacggag catgcagaag aaacaggccc tgctggaggc
     2341 tgtcgccggc aaggaggggg acagcttcaa cacacagtgc ctcaagctgg cctagctgcc
     2401 tccaacacac tacgtcagaa ggacccgggt ctttgaaact gtgtcttgaa gctaatgtat
     2461 taatgtgaca tggaggaact caataaaact cctgcttca
//
