LOCUS       NM_021942               4523 bp    mRNA    linear   PRI 17-SEP-2024
DEFINITION  Homo sapiens trafficking protein particle complex subunit 11
            (TRAPPC11), transcript variant 1, mRNA.
ACCESSION   NM_021942
VERSION     NM_021942.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Kopcilova,J., Ptackova,H., Kramarova,T., Fajkusova,L., Reblova,K.,
            Zeman,J., Honzik,T., Zdrazilova,L., Zamecnik,J., Balazova,P.,
            Viestova,K., Kolnikova,M., Hansikova,H. and Zidkova,J.
  TITLE     Large TRAPPC11 gene deletions as a cause of muscular dystrophy and
            their estimated genesis
  JOURNAL   J Med Genet 61 (9), 908-913 (2024)
   PUBMED   38955476
  REMARK    GeneRIF: Large TRAPPC11 gene deletions as a cause of muscular
            dystrophy and their estimated genesis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Justel,M., Jou,C., Sariego-Jamardo,A., Julia-Palacios,N.A.,
            Ortez,C., Poch,M.L., Hedrera-Fernandez,A., Gomez-Martin,H.,
            Codina,A., Dominguez-Carral,J., Muxart,J., Hernandez-Lain,A.,
            Vila-Bedmar,S., Zulaica,M., Cancho-Candela,R., Castro,M.D.C., de la
            Osa-Langreo,A., Pena-Valenceja,A., Marcos-Vadillo,E.,
            Prieto-Matos,P., Pascual-Pascual,S.I., Lopez de Munain,A.,
            Camacho,A., Estevez-Arias,B., Musokhranova,U., Olivella,M.,
            Oyarzabal,A., Jimenez-Mallebrera,C., Dominguez-Gonzalez,C.,
            Nascimento,A., Garcia-Cazorla,A. and Natera-de Benito,D.
  TITLE     Expanding the phenotypic spectrum of TRAPPC11-related muscular
            dystrophy: 25 Roma individuals carrying a founder variant
  JOURNAL   J Med Genet 60 (10), 965-973 (2023)
   PUBMED   37197784
  REMARK    GeneRIF: Expanding the phenotypic spectrum of TRAPPC11-related
            muscular dystrophy: 25 Roma individuals carrying a founder variant.
REFERENCE   3  (bases 1 to 4523)
  AUTHORS   Stanga,D., Zhao,Q., Milev,M.P., Saint-Dic,D., Jimenez-Mallebrera,C.
            and Sacher,M.
  TITLE     TRAPPC11 functions in autophagy by recruiting ATG2B-WIPI4/WDR45 to
            preautophagosomal membranes
  JOURNAL   Traffic 20 (5), 325-345 (2019)
   PUBMED   30843302
  REMARK    GeneRIF: TRAPPC11 recruits ATG2B-WIPI4/WDR45 to preautophagosomal
            membranes.  Fibroblasts from a patient with TRAPPC11 mutations
            failed to recruit ATG2B-WIPI4.
REFERENCE   4  (bases 1 to 4523)
  AUTHORS   Larson,A.A., Baker,P.R. 2nd, Milev,M.P., Press,C.A., Sokol,R.J.,
            Cox,M.O., Lekostaj,J.K., Stence,A.A., Bossler,A.D., Mueller,J.M.,
            Prematilake,K., Tadjo,T.F., Williams,C.A., Sacher,M. and Moore,S.A.
  TITLE     TRAPPC11 and GOSR2 mutations associate with hypoglycosylation of
            alpha-dystroglycan and muscular dystrophy
  JOURNAL   Skelet Muscle 8 (1), 17 (2018)
   PUBMED   29855340
  REMARK    GeneRIF: Recessive mutations in TRAPPC11 and GOSR2 are associated
            with congenital muscular dystrophy and hypoglycosylation of
            alpha-dystroglycan.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4523)
  AUTHORS   DeRossi,C., Vacaru,A., Rafiq,R., Cinaroglu,A., Imrie,D., Nayar,S.,
            Baryshnikova,A., Milev,M.P., Stanga,D., Kadakia,D., Gao,N., Chu,J.,
            Freeze,H.H., Lehrman,M.A., Sacher,M. and Sadler,K.C.
  TITLE     trappc11 is required for protein glycosylation in zebrafish and
            humans
  JOURNAL   Mol Biol Cell 27 (8), 1220-1234 (2016)
   PUBMED   26912795
  REMARK    GeneRIF: TRAPPC11 role in protein glycosylation and lipid-linked
            oligosaccharides biosynthesis
REFERENCE   6  (bases 1 to 4523)
  AUTHORS   Kim,J.J., Lipatova,Z. and Segev,N.
  TITLE     TRAPP Complexes in Secretion and Autophagy
  JOURNAL   Front Cell Dev Biol 4, 20 (2016)
   PUBMED   27066478
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 4523)
  AUTHORS   Bogershausen,N., Shahrzad,N., Chong,J.X., von Kleist-Retzow,J.C.,
            Stanga,D., Li,Y., Bernier,F.P., Loucks,C.M., Wirth,R.,
            Puffenberger,E.G., Hegele,R.A., Schreml,J., Lapointe,G., Keupp,K.,
            Brett,C.L., Anderson,R., Hahn,A., Innes,A.M., Suchowersky,O.,
            Mets,M.B., Nurnberg,G., McLeod,D.R., Thiele,H., Waggoner,D.,
            Altmuller,J., Boycott,K.M., Schoser,B., Nurnberg,P., Ober,C.,
            Heller,R., Parboosingh,J.S., Wollnik,B., Sacher,M. and Lamont,R.E.
  TITLE     Recessive TRAPPC11 mutations cause a disease spectrum of limb
            girdle muscular dystrophy and myopathy with movement disorder and
            intellectual disability
  JOURNAL   Am J Hum Genet 93 (1), 181-190 (2013)
   PUBMED   23830518
  REMARK    GeneRIF: Homozygous mutations in the membrane trafficking component
            TRAPPC11 causes a form of autosomal-recessive, slowly progressive
            limb girdle muscular dystrophy with childhood onset and high serum
            creatine kinase.
REFERENCE   8  (bases 1 to 4523)
  AUTHORS   Yu,S. and Liang,Y.
  TITLE     A trapper keeper for TRAPP, its structures and functions
  JOURNAL   Cell Mol Life Sci 69 (23), 3933-3944 (2012)
   PUBMED   22669257
  REMARK    Review article
REFERENCE   9  (bases 1 to 4523)
  AUTHORS   Scrivens,P.J., Noueihed,B., Shahrzad,N., Hul,S., Brunet,S. and
            Sacher,M.
  TITLE     C4orf41 and TTC-15 are mammalian TRAPP components with a role at an
            early stage in ER-to-Golgi trafficking
  JOURNAL   Mol Biol Cell 22 (12), 2083-2093 (2011)
   PUBMED   21525244
REFERENCE   10 (bases 1 to 4523)
  AUTHORS   Wendler,F., Gillingham,A.K., Sinka,R., Rosa-Ferreira,C.,
            Gordon,D.E., Franch-Marro,X., Peden,A.A., Vincent,J.P. and Munro,S.
  TITLE     A genome-wide RNA interference screen identifies two novel
            components of the metazoan secretory pathway
  JOURNAL   EMBO J 29 (2), 304-314 (2010)
   PUBMED   19942856
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX648499.1, BX647127.1 and
            AI034264.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_021942.5.
            
            Summary: The protein encoded by this gene is a subunit of the TRAPP
            (transport protein particle) tethering complex, which functions in
            intracellular vesicle trafficking. This subunit is involved in
            early stage endoplasmic reticulum-to-Golgi vesicle transport.
            Alternative splicing of this gene results in multiple transcript
            variants. [provided by RefSeq, Jan 2013].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK096345.1, BX647127.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000334690.11/ ENSP00000335371.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2320              BX648499.1         2-2321
            2321-4521           BX647127.1         2318-4518
            4522-4523           AI034264.1         16-17               c
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q35.1"
     gene            1..4523
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /note="trafficking protein particle complex subunit 11"
                     /db_xref="GeneID:60684"
                     /db_xref="HGNC:HGNC:25751"
                     /db_xref="MIM:614138"
     exon            1..155
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            156..380
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     CDS             177..3578
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /note="isoform a is encoded by transcript variant 1;
                     gryzun homolog; foie gras homolog; trafficking protein
                     particle complex 11"
                     /codon_start=1
                     /product="trafficking protein particle complex subunit 11
                     isoform a"
                     /protein_id="NP_068761.4"
                     /db_xref="CCDS:CCDS34112.1"
                     /db_xref="GeneID:60684"
                     /db_xref="HGNC:HGNC:25751"
                     /db_xref="MIM:614138"
                     /translation="MSPTQWDFPVELCCRPMAFVTLTGLDVVYNAVHRAVWDAFCANR
                     RADRVPISFKVLPGDHEYPKCRPKRTSYEWYIPKGILKTGWMNKHLNLVPALVVVFYE
                     LDWDEPQWKEKQSECATRVEIVRQSLQGRNTKVAVVLIQKKTPLPPGEDVIASERAAA
                     LCNACELSGKSLFVLPHTDHLVGYIIRLENAFYEHAQTYYYTEIRRVKSHKEFLNKTT
                     HQLLFVRHQFKIAFFSELKQDTQNALKNYRTAYNLVHELRAHETNILEIKTMAGFINY
                     KICRLCFQHNTPLDAIAQFRKHIDLCKKKIGSAELSFEHDAWMSKQFQAFGDLFDEAI
                     KLGLTAIQTQNPGFYYQQAAYYAQERKQLAKTLCNHEASVMYPNPDPLETQTGVLDFY
                     GQRSWRQGILSFDLSDPEKEKVGILAIQLKERNVVHSEIIITLLSNAVAQFKKYKCPR
                     MKSHLMVQMGEEYYYAKDYTKALKLLDYVMCDYRSEGWWTLLTSVLTTALKCSYLMAQ
                     LKDYITYSLELLGRASTLKDDQKSRIEKNLINVLMNESPDPEPDCDILAVKTAQKLWA
                     DRISLAGSNIFTIGVQDFVPFVQCKAKFHAPSFHVDVPVQFDIYLKADCPHPIRFSKL
                     CVSFNNQEYNQFCVIEEASKANEVLENLTQGKMCLVPGKTRKLLFKFVAKTEDVGKKI
                     EITSVDLALGNETGRCVVLNWQGGGGDAASSQEALQAARSFKRRPKLPDNEVHWDSII
                     IQASTMIISRVPNISVHLLHEPPALTNEMYCLVVTVQSHEKTQIRDVKLTAGLKPGQD
                     ANLTQKTHVTLHGTELCDESYPALLTDIPVGDLHPGEQLEKMLYVRCGTVGSRMFLVY
                     VSYLINTTVEEKEIVCKCHKDETVTIETVFPFDVAVKFVSTKFEHLERVYADIPFLLM
                     TDLLSASPWALTIVSSELQLAPSMTTVDQLESQVDNVILQTGESASECFCLQCPSLGN
                     IEGGVATGHYIISWKRTSAMENIPIITTVITLPHVIVENIPLHVNADLPSFGRVRESL
                     PVKYHLQNKTDLVQDVEISVEPSDAFMFSGLKQIRLRILPGTEQEMLYNFYPLMAGYQ
                     QLPSLNINLLRFPNFTNQLLRRFIPTSIFVKPQGRLMDDTSIAAA"
     exon            381..550
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            551..621
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            622..736
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            737..836
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            837..910
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            911..1007
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1141
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1289
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            1290..1383
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1463
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1542
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            1543..1597
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            1598..1743
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            1744..1805
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            1806..1938
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            1939..2069
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            2070..2225
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            2226..2413
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            2414..2562
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            2563..2684
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            2685..2804
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            2805..2870
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            2871..3027
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            3028..3139
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            3140..3231
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            3232..3365
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            3366..3533
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     exon            3534..4523
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4503..4508
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /note="hexamer: AATAAA"
     polyA_site      4523
                     /gene="TRAPPC11"
                     /gene_synonym="C4orf41; FOIGR; GRY; LGMD2S; LGMDR18"
                     /note="major polyA site"
ORIGIN      
        1 gggtacaggg aagtctcgcc tgttggaact ggctgtgggg ctgcggcggt ggggacgggc
       61 cacggcgttc tgtgacatcc ccccgcctcc cctcgtcttc tcccggctgg cggcgaccgg
      121 cctcagctgc agcgggcccg gggcgtgggg cctgggtttt ttgtgacatc gtaaacatga
      181 gccccacaca gtgggacttc cctgtggaat tatgttgccg gcctatggcc tttgttactc
      241 taacgggcct ggatgtagtt tataatgcag tccatcgagc tgtctgggac gccttctgtg
      301 caaatcggag agctgatcga gtaccaattt ctttcaaggt gctcccaggt gaccatgagt
      361 atcccaaatg tagacccaag agaacttcat atgagtggta cattcctaaa gggatcttaa
      421 agactggctg gatgaataag catctgaatc tggtgccagc cctggtggtt gtgttctatg
      481 aactggactg ggatgagcct cagtggaaag aaaagcagtc tgagtgcgcc accagagtgg
      541 aaatagtcag gcaaagttta caaggaagaa acacaaaagt tgcagtggtt ctgattcaga
      601 agaaaacccc tttgccccca ggagaagatg tcattgcttc agaaagggct gcagctttat
      661 gcaatgcatg tgaactctca ggaaagtctt tgtttgtact gccgcacact gaccaccttg
      721 tgggttatat tataagattg gaaaatgcct tttatgaaca tgcacagact tattactaca
      781 ctgagatcag aagagtgaaa tctcataaag aatttttgaa taaaacaaca caccagcttt
      841 tatttgttag gcatcagttc aaaatagctt tcttcagtga gttgaaacaa gatacacaaa
      901 atgcgctgaa gaattatagg accgcctata atcttgtaca cgaattgaga gcccatgaaa
      961 ctaatattct ggaaattaag actatggcag gatttataaa ctacaagatc tgtaggctgt
     1021 gttttcaaca caacacccca ttggatgcaa ttgctcagtt ccgaaaacac atcgacttgt
     1081 gtaagaaaaa gattggaagt gcagagctgt cttttgagca tgatgcatgg atgtctaaac
     1141 aattccaggc ctttggagat ttatttgatg aagctattaa gttagggtta acagctattc
     1201 aaactcagaa tcctggtttc tattaccagc aggcagcata ctatgcccag gagcggaaac
     1261 agcttgcaaa aaccctctgt aaccacgaag cttctgtaat gtatcccaat cctgatccct
     1321 tagaaacaca aacaggcgtt cttgactttt atggacaaag atcatggcga caaggaatac
     1381 taagttttga tctttctgat cctgaaaaag aaaaggtggg aattcttgcc attcagctga
     1441 aggagagaaa tgttgttcac tctgagataa tcataactct tctgagcaat gctgttgcac
     1501 agttcaagaa gtataagtgc ccgcgaatga aaagtcacct aatggttcag atgggagagg
     1561 aatattatta cgcaaaggat tataccaaag ctttgaagtt gctggattat gtgatgtgtg
     1621 attatcggag tgaaggatgg tggactctgc tcacttctgt attaactaca gctctgaagt
     1681 gctcctacct catggcccaa ttaaaggatt acattactta ctccctagaa ctccttggta
     1741 gagcttcaac tctgaaagat gaccagaagt ctcggataga aaagaacctc ataaatgttt
     1801 taatgaatga aagtcctgat ccagaacccg actgtgatat cttagctgtg aaaactgctc
     1861 agaagctgtg ggcagaccga atttctctgg ctggcagcaa tattttcaca ataggagtac
     1921 aggactttgt gccatttgtg cagtgcaaag ccaagtttca tgccccaagt tttcatgttg
     1981 atgttcctgt tcagtttgat atttatctga aggctgattg tccacatccc attaggtttt
     2041 ccaagctctg tgtcagcttt aataatcagg aatacaacca gttctgtgta atagaagaag
     2101 catccaaagc aaatgaagtt ttagaaaatc tgactcaagg aaagatgtgc ctagttcctg
     2161 gcaaaacaag aaaactgtta tttaagtttg ttgcaaaaac tgaagatgtg ggaaagaaaa
     2221 ttgagattac ttcagtggat cttgctctgg gcaatgagac gggaagatgt gtggttttaa
     2281 attggcaggg aggaggagga gatgctgctt cctcccaaga agccttacag gcagctcggt
     2341 ctttcaaaag gcgacctaag ctacctgaca atgaagttca ctgggacagc attataattc
     2401 aggcaagcac aatgatcata tccagagtcc caaacatttc tgtacatctg ctacatgaac
     2461 cccctgcact gactaatgaa atgtattgtt tggttgtgac tgttcagtcc catgaaaaga
     2521 cccaaatcag agatgtgaag ctcaccgctg gcttaaaacc aggacaggat gccaatttaa
     2581 ctcagaagac tcacgtgact cttcatggaa cagaactgtg tgatgaatcc tacccggctt
     2641 tactcactga cattcctgtt ggagacttac atccagggga acagctggaa aaaatgttgt
     2701 atgttcgctg tggaacagtg ggttccagaa tgtttcttgt atatgtttct tacctgataa
     2761 atacaaccgt tgaagaaaaa gaaattgttt gcaagtgtca caaggatgaa actgtaacaa
     2821 ttgaaacagt ctttccattt gatgttgcgg ttaaatttgt ttctaccaag tttgagcacc
     2881 tggaaagggt ttatgctgac atcccctttc tgttgatgac ggacctctta agtgcctcac
     2941 cctgggccct cactattgtt tccagtgagc tccagcttgc tccatccatg accacagtgg
     3001 accagctcga gtctcaagtg gacaatgtta tcttacagac tggagagagt gctagtgaat
     3061 gcttttgtct tcaatgccca tctcttggaa atattgaagg tggagtagca accgggcatt
     3121 atattatctc ttggaaaagg acctcagcaa tggagaatat ccccatcatc acaactgtca
     3181 tcactctgcc gcacgtgatt gtggagaata tccctctcca tgtgaatgca gatctgccgt
     3241 catttgggcg tgtcagagag tcgttacctg tcaagtatca cctacagaat aagaccgact
     3301 tagttcaaga tgtagaaatt tctgtggagc ccagtgatgc cttcatgttc tcaggtctca
     3361 aacagattcg attacgtatc ctccctggca cggagcagga aatgctatat aatttctatc
     3421 ctctgatggc tggataccag cagctgccat ctctcaacat caacttgctt agatttccta
     3481 acttcacaaa tcagctgctc aggcgtttta tacctaccag tatttttgtc aagccacagg
     3541 gtcgactcat ggatgatacc tctattgctg ctgcatgatg ttcaagaccg gcccttggct
     3601 gttgttacag agatgttggg cagagctatg caggtgtttc attgtgaact ctagctttga
     3661 tcatggtaaa aagttaacct tttctatttt ttaatggatg ttataccaac tattcagagg
     3721 aactcatact tcaaaaatat taggaaaatc tgtcttatag tttctctaat aaatatctga
     3781 aatctcagta cgacatgaaa gaatgtcaga ccattgttat tgttgaaagt catttgatga
     3841 atggtaaatt ctatgaaaag taagtgattt gcatgtataa tatcaggaaa attaagcatc
     3901 ccaagtgtga ctggacaaag agagcagatg caccagtgcc tgtgccataa agttccgaat
     3961 cccccatgtg tctctttcag agctggccag accggaaata aatcattctc ataaattcag
     4021 tgtgtactca gaacacatac acaacaacat agggagttgt atgactgata cggaaaactt
     4081 ccagaaagtt ttaatcaaag cagtttaatt aaggtatcaa aaatatcttt gcttactatc
     4141 aagaagtgtc aaataggttc agcttgctgc caaaatatgg atcatttatg aagcaggttc
     4201 atattttaga ggtgttaata aaatcctcat cggaaaagat ccaaagtgca aggatttgat
     4261 tataaacata atttcctaga ctgaaagttt ttggaaaaga tgcagggtct gagtcaggcc
     4321 ttctggttat attgtgcagt ttcaaaagaa ctatttaaaa ctcttgaaaa ctcatgtaaa
     4381 taaaaatcat agggtgaaaa ttgtatttgt taaaatacct taataattta aaatgacctg
     4441 atttcctgga aaattttatt attcaaaagg tggaggcatt gtaaaaagga aatagtgatg
     4501 taaataaaca tgttctcttt caa
//
