LOCUS       NM_003274               6986 bp    mRNA    linear   PRI 12-MAR-2023
DEFINITION  Homo sapiens trafficking protein particle complex subunit 10
            (TRAPPC10), transcript variant 1, mRNA.
ACCESSION   NM_003274
VERSION     NM_003274.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Rawlins LE, Almousa H, Khan S, Collins SC, Milev MP, Leslie J,
            Saint-Dic D, Khan V, Hincapie AM, Day JO, McGavin L, Rowley C,
            Harlalka GV, Vancollie VE, Ahmad W, Lelliott CJ, Gul A, Yalcin B,
            Crosby AH, Sacher M and Baple EL.
  TITLE     Biallelic variants in TRAPPC10 cause a microcephalic TRAPPopathy
            disorder in humans and mice
  JOURNAL   PLoS Genet 18 (3), e1010114 (2022)
   PUBMED   35298461
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   Cuenca A, Insinna C, Zhao H, John P, Weiss MA, Lu Q, Walia V,
            Specht S, Manivannan S, Stauffer J, Peden AA and Westlake CJ.
  TITLE     The C7orf43/TRAPPC14 component links the TRAPPII complex to Rabin8
            for preciliary vesicle tethering at the mother centriole during
            ciliogenesis
  JOURNAL   J Biol Chem 294 (42), 15418-15434 (2019)
   PUBMED   31467083
REFERENCE   3  (bases 1 to 6986)
  AUTHORS   Boldt K, van Reeuwijk J, Lu Q, Koutroumpas K, Nguyen TM, Texier Y,
            van Beersum SE, Horn N, Willer JR, Mans DA, Dougherty G, Lamers IJ,
            Coene KL, Arts HH, Betts MJ, Beyer T, Bolat E, Gloeckner CJ,
            Haidari K, Hetterschijt L, Iaconis D, Jenkins D, Klose F, Knapp B,
            Latour B, Letteboer SJ, Marcelis CL, Mitic D, Morleo M, Oud MM,
            Riemersma M, Rix S, Terhal PA, Toedt G, van Dam TJ, de Vrieze E,
            Wissinger Y, Wu KM, Apic G, Beales PL, Blacque OE, Gibson TJ,
            Huynen MA, Katsanis N, Kremer H, Omran H, van Wijk E, Wolfrum U,
            Kepes F, Davis EE, Franco B, Giles RH, Ueffing M, Russell RB and
            Roepman R.
  CONSRTM   UK10K Rare Diseases Group
  TITLE     An organelle-specific protein landscape identifies novel diseases
            and molecular mechanisms
  JOURNAL   Nat Commun 7, 11491 (2016)
   PUBMED   27173435
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6986)
  AUTHORS   Kim JJ, Lipatova Z and Segev N.
  TITLE     TRAPP Complexes in Secretion and Autophagy
  JOURNAL   Front Cell Dev Biol 4, 20 (2016)
   PUBMED   27066478
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6986)
  AUTHORS   Lafreniere RG, Kibar Z, Rochefort DL, Han FY, Fon EA, Dube MP, Kang
            X, Baird S, Korneluk RG, Rommens JM and Rouleau GA.
  TITLE     Genomic structure of the human GT334 (EHOC-1) gene mapping to
            21q22.3
  JOURNAL   Gene 198 (1-2), 313-321 (1997)
   PUBMED   9370297
REFERENCE   6  (bases 1 to 6986)
  AUTHORS   Nagamine K, Kudoh J, Minoshima S, Kawasaki K, Asakawa S, Ito F and
            Shimizu N.
  TITLE     Genomic organization and complete nucleotide sequence of the human
            PWP2 gene on chromosome 21
  JOURNAL   Genomics 42 (3), 528-531 (1997)
   PUBMED   9205129
REFERENCE   7  (bases 1 to 6986)
  AUTHORS   Nagamine K, Kudoh J, Kawasaki K, Minoshima S, Asakawa S, Ito F and
            Shimizu N.
  TITLE     Genomic organization and complete nucleotide sequence of the TMEM1
            gene on human chromosome 21q22.3
  JOURNAL   Biochem Biophys Res Commun 235 (1), 185-190 (1997)
   PUBMED   9196060
REFERENCE   8  (bases 1 to 6986)
  AUTHORS   Nagamine K, Kudoh J, Minoshima S, Kawasaki K, Asakawa S, Ito F and
            Shimizu N.
  TITLE     Isolation of cDNA for a novel human protein KNP-I that is
            homologous to the E. coli SCRP-27A protein from the autoimmune
            polyglandular disease type I (APECED) region of chromosome 21q22.3
  JOURNAL   Biochem Biophys Res Commun 225 (2), 608-616 (1996)
   PUBMED   8753807
REFERENCE   9  (bases 1 to 6986)
  AUTHORS   Yamakawa K, Mitchell S, Hubert R, Chen XN, Colbern S, Huo YK,
            Gadomski C, Kim UJ and Korenberg JR.
  TITLE     Isolation and characterization of a candidate gene for progressive
            myoclonus epilepsy on 21q22.3
  JOURNAL   Hum Mol Genet 4 (4), 709-716 (1995)
   PUBMED   7633421
REFERENCE   10 (bases 1 to 6986)
  AUTHORS   Tekendo-Ngongang,C., Muenke,M. and Kruszka,P.
  TITLE     Holoprosencephaly Overview
  JOURNAL   (in) Adam MP, Mirzaa GM, Pagon RA, Wallace SE, Bean LJH, Gripp KW
            and Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301702
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB001523.1, AL559301.3,
            BC101728.1 and AB001517.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_003274.4.
            
            Summary: The protein encoded by this gene is a transmembrane
            protein found in the cis-Golgi complex. The encoded protein is part
            of the multisubunit transport protein particle (TRAPP) complex and
            may be involved in vesicular transport from the endoplasmic
            reticulum to the Golgi. Mutations in this gene could be responsible
            for the Unverricht-Lundborg type of progressive myoclonus epilepsy,
            or for autoimmune polyglandular disease type 1. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U61500.1, U19252.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000291574.9/ ENSP00000291574.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                AB001523.1         25393-25408
            17-66               AL559301.3         7-56
            67-96               AL559301.3         58-87
            97-3990             BC101728.1         1-3894
            3991-6986           AB001517.1         2602-5597
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q22.3"
     gene            1..6986
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /note="trafficking protein particle complex subunit 10"
                     /db_xref="GeneID:7109"
                     /db_xref="HGNC:HGNC:11868"
                     /db_xref="MIM:602103"
     exon            1..252
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     CDS             186..3965
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /note="isoform a is encoded by transcript variant 1;
                     transmembrane protein 1; TRAPP 130 kDa subunit;
                     trafficking protein particle complex subunit 130; epilepsy
                     holoprosencephaly candidate-1 protein; trafficking protein
                     particle complex 10; transport protein particle subunit
                     TMEM1; TRAPP subunit TMEM1; trafficking protein particle
                     complex subunit TMEM1"
                     /codon_start=1
                     /product="trafficking protein particle complex subunit 10
                     isoform a"
                     /protein_id="NP_003265.3"
                     /db_xref="CCDS:CCDS13704.1"
                     /db_xref="GeneID:7109"
                     /db_xref="HGNC:HGNC:11868"
                     /db_xref="MIM:602103"
                     /translation="MDASEEPLPPVIYTMENKPIVTCAGDQNLFTSVYPTLSQQLPRE
                     PMEWRRSYGRAPKMIHLESNFVQFKEELLPKEGNKALLTFPFLHIYWTECCDTEVYKA
                     TVKDDLTKWQNVLKAHSSVDWLIVIVENDAKKKNKTNILPRTSIVDKIRNDFCNKQSD
                     RCVVLSDPLKDSSRTQESWNAFLTKLRTLLLMSFTKNLGKFEDDMRTLREKRTEPGWS
                     FCEYFMVQEELAFVFEMLQQFEDALVQYDELDALFSQYVVNFGAGDGANWLTFFCQPV
                     KSWNGLILRKPIDMEKRESIQRREATLLDLRSYLFSRQCTLLLFLQRPWEVAQRALEL
                     LHNCVQELKLLEVSVPPGALDCWVFLSCLEVLQRIEGCCDRAQIDSNIAHTVGLWSYA
                     TEKLKSLGYLCGLVSEKGPNSEDLNRTVDLLAGLGAERPETANTAQSPYKKLKEALSS
                     VEAFEKHYLDLSHATIEMYTSIGRIRSAKFVGKDLAEFYMRKKAPQKAEIYLQGALKN
                     YLAEGWALPITHTRKQLAECQKHLGQIENYLQTSSLLASDHHLTEEERKHFCQEILDF
                     ASQPSDSPGHKIVLPMHSFAQLRDLHFDPSNAVVHVGGVLCVEITMYSQMPVPVHVEQ
                     IVVNVHFSIEKNSYRKTAEWLTKHKTSNGIINFPPETAPFPVSQNSLPALELYEMFER
                     SPSDNSLNTTGIICRNVHMLLRRQESSSSLEMPSGVALEEGAHVLRCSHVTLEPGANQ
                     ITFRTQAKEPGTYTLRQLCASVGSVWFVLPHIYPIVQYDVYSQEPQLHVEPLADSLLA
                     GIPQRVKFTVTTGHYTIKNGDSLQLSNAEAMLILCQAESRAVVYSNTREQSSEAALRI
                     QSSDKVTSISLPVAPAYHVIEFELEVLSLPSAPALGGESDMLGMAEPHRKHKDKQRTG
                     RCMVTTDHKVSIDCPWSIYSTVIALTFSVPFRTTHSLLSSGTRKYVQVCVQNLSELDF
                     QLSDSYLVDTGDSTDLQLVPLNTQSQQPIYSKQSVFFVWELKWTEEPPPSLHCRFSVG
                     FSPASEEQLSISLKPYTYEFKVENFFTLYNVKAEIFPPSGMEYCRTGSLCSLEVLITR
                     LSDLLEVDKDEALTESDEHFSTKLMYEVVDNSSNWAVCGKSCGVISMPVAARATHRVH
                     MEVMPLFAGYLPLPDVRLFKYLPHHSAHSSQLDADSWIENDSLSVDKHGDDQPDSSSL
                     KSRGSVHSACSSEHKGLPMPRLQALPAGQVFNSSSGTQVLVIPSQDDHVLEVSVT"
     exon            253..334
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            335..470
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            471..667
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            668..863
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            864..975
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1223
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1370
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1485
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..1562
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            1563..1654
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..1795
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            1796..1908
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            1909..2423
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            2424..2565
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            2566..2724
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            2725..2954
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            2955..3055
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            3056..3182
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            3183..3353
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            3354..3531
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            3532..3726
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     exon            3727..6986
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5143..5148
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /note="hexamer: ATTAAA"
     polyA_site      5167
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /note="major polyA site"
     regulatory      6966..6971
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /note="hexamer: AATACA"
     polyA_site      6986
                     /gene="TRAPPC10"
                     /gene_synonym="EHOC-1; EHOC1; GT334; NEDMISS; TMEM1;
                     TRS130; TRS30"
                     /note="major polyA site"
ORIGIN      
        1 ggcgccgagg ccggaagtgg ctgaggccgg cagcagcggg cggcagctgc ggcgcaaccg
       61 gctccggagc tgcctggcgc ggccgggcgg gcggcgccgc tcaggctcgg gctccggctg
      121 ggcccggcgc ggcctcgggg ctgcccatgg ggcgcggggg gccgggccgg tgacgccgga
      181 cgcccatgga cgcctctgag gagccgctgc cgccggtgat ctacaccatg gagaacaagc
      241 ccatcgtcac ctgtgctgga gatcagaatt tatttacctc tgtttatcca acgctctctc
      301 agcagcttcc aagagaacca atggaatgga gaaggtccta tggccgggct ccgaagatga
      361 ttcacctaga gtctaacttt gttcaattca aagaggagct gctgcccaaa gaaggaaaca
      421 aagctctgct cacgtttccc ttcctccata tttactggac agagtgctgt gataccgaag
      481 tgtataaagc tacagtaaaa gatgacctca ccaagtggca gaatgttctg aaggctcata
      541 gctctgtgga ctggttaata gtgatagttg aaaatgatgc caagaaaaaa aacaaaacca
      601 acatccttcc ccgaacctct attgtggaca aaataagaaa tgatttttgt aataaacaga
      661 gtgacaggtg tgttgtgctc tccgacccct tgaaggactc ttctcgaact caggaatcct
      721 ggaatgcctt cctgaccaaa ctcaggacat tgcttcttat gtcttttacc aaaaacctag
      781 gcaagtttga ggatgacatg agaaccttga gggagaagag gactgagcca ggctggagct
      841 tttgtgaata tttcatggtt caggaggagc ttgcctttgt tttcgagatg ctgcagcagt
      901 tcgaggacgc cctggtgcag tacgacgaac tggacgccct cttctctcag tatgtggtca
      961 acttcggggc cggggatggt gccaactggc tgactttttt ctgccagcca gtgaagagct
     1021 ggaacggatt gatcctccga aaacccatag atatggagaa gcgggaatcg atccagaggc
     1081 gagaagccac cctgttagat ctgcgcagtt acctgttctc tcgccagtgc accttgctgc
     1141 tcttcctgca gaggccgtgg gaggtggccc agcgcgccct agagctgctg cacaactgcg
     1201 tgcaggaact gaagctctta gaagtctctg tcccacctgg tgctctggac tgctgggtgt
     1261 ttctgagctg tctggaggtg ttgcagagga tagaaggctg ctgtgaccgg gcacagatcg
     1321 actcaaacat tgcccacact gtggggctat ggagctatgc cacagaaaag ttaaagtcct
     1381 tgggctatct atgtggactt gtgtcagaga aaggacctaa ctcagaagat ctcaacagga
     1441 cagttgacct tttggcaggt ttgggagctg agcgaccaga aacagccaac acagctcaga
     1501 gtccttataa gaaactgaaa gaagcattat cgtcagtgga agcttttgaa aaacactact
     1561 tagatttgtc ccatgccacc attgaaatgt atacaagcat tgggaggatt cgatctgcta
     1621 agtttgttgg aaaagatctg gcagagtttt acatgaggaa aaaggctcca caaaaggcag
     1681 aaatctatct tcaaggagca ctgaaaaact acctggctga gggctgggca ctccccatca
     1741 cacacacaag gaagcagctg gccgaatgtc aaaagcacct tggacaaatt gaaaactacc
     1801 tgcagaccag cagcctctta gccagtgacc accacctcac tgaagaggag cgcaagcact
     1861 tctgccagga gatacttgac tttgccagcc agccgtcaga cagcccaggt cataagatag
     1921 tgctacccat gcattccttt gcacaactgc gagatctcca ttttgatccc tccaatgccg
     1981 tggtccacgt gggcggcgtt ttgtgcgttg agataaccat gtacagccag atgcctgtgc
     2041 ctgttcacgt ggagcagatt gtggtcaatg tccacttcag cattgagaaa aacagctacc
     2101 ggaagactgc ggagtggctt accaagcaca agacgtccaa tgggatcatt aactttccac
     2161 ccgagaccgc acctttccct gtatcccaaa acagtttgcc cgcgctggag ttgtatgaaa
     2221 tgtttgagag aagcccatct gataactcct tgaacacgac tgggattatc tgcagaaacg
     2281 tccacatgct cctgagaagg caggagagca gctcctctct agagatgccc tcaggggtgg
     2341 ctctggagga gggtgcccac gtgctgaggt gcagccacgt gaccctggaa ccaggggcca
     2401 accagataac attcaggact caggccaagg aacctggaac gtatacactc aggcagctgt
     2461 gcgcctcggt gggctccgtg tggttcgtcc tccctcacat ctaccccatt gtgcagtacg
     2521 acgtgtactc acaggagccc cagctgcacg tggagccgct ggctgatagc cttctggcag
     2581 gcattcctca gagagtcaag ttcactgtca ctaccggcca ttatacgata aagaatggag
     2641 acagcctgca gcttagcaat gccgaagcca tgctcatcct gtgccaggcg gagagcaggg
     2701 ctgtggtcta ctccaacacg agagaacagt cttctgaggc cgcgctccgg attcagtcct
     2761 ccgacaaggt cacgagcatc agtctgcctg ttgcgcctgc gtaccacgtg atcgaatttg
     2821 aactggaagt tctctcttta ccttcagccc cagcactcgg aggggagagt gacatgctgg
     2881 ggatggcaga gccccacagg aagcataagg acaaacagag aactggccgc tgcatggtta
     2941 ccacagacca caaagtgtcg attgactgcc cgtggtccat ctactccaca gtcatcgcac
     3001 tgaccttcag cgtacccttc aggaccacac acagcctcct gtcctcagga acacggaaat
     3061 atgttcaagt ttgtgtccag aatttgtcag aacttgactt tcagctgtca gatagttatc
     3121 ttgtagatac cggtgatagt accgacctgc aactagtacc actgaacacg cagtcccagc
     3181 agcccatcta cagcaagcag tcggtgttct tcgtctggga actcaagtgg acagaagagc
     3241 ctcccccttc tctgcattgc cggttctctg ttggattttc cccagcttct gaggaacagc
     3301 tgtctatctc cttaaagccg tatacttatg aatttaaagt ggaaaatttt tttacattat
     3361 acaacgtgaa ggctgagatc tttccccctt cgggaatgga gtattgcaga acaggctccc
     3421 tctgctccct ggaggttttg atcacgaggc tctcagacct cttggaggtg gataaagatg
     3481 aagcactgac tgaatctgat gagcattttt cgacaaagct tatgtatgaa gttgtcgaca
     3541 acagtagcaa ctgggcagtg tgtgggaaaa gctgcggtgt catctccatg ccagtggctg
     3601 ctcgggccac tcacagggtc cacatggaag tgatgccgct cttcgccggg tatctccccc
     3661 tgcccgacgt caggctgttc aagtacctcc cccatcattc tgcacactcc tcccaactgg
     3721 acgctgacag ctggatagaa aacgacagcc tgtcagtaga caagcacggg gacgaccagc
     3781 cggacagcag cagcctcaag agcaggggca gcgtgcattc ggcctgcagc agcgagcaca
     3841 aaggcctacc catgccccgg ctgcaggcac tgccggccgg ccaggtcttc aactccagct
     3901 cgggcacaca agtcctggtc atccccagcc aagatgacca cgtcctggaa gtcagtgtaa
     3961 catgacaacg ccagggtgaa cacacgccac ttcccagcta ggagtgcact ttatgggact
     4021 gtgactggac tcttccgttc tggctccagc cagaccttca gtggtcctgc ctggccgtgg
     4081 ggacatcaga gagtgtcatc acgcagctgg ccagctgagt tctgttgttg ttttcatgcc
     4141 gcctgtgatc tcagattcct gcttttctca ccccgtcccc atgctggtgt ccgacgccgc
     4201 ttactcagag ccctggcctc cctcccccta cctcacacgc tgctcatgaa agtttccacc
     4261 cacgctgtct ccacggaaca gcctccgtct gctggctctt cgtggaaggc catttgtctt
     4321 tcaggtagac actcagcagc cctcacggtc ttagtgacgt gtgtgccttt ctggtcacac
     4381 agctgcccag tttcctgatc ggggtggatt tgtgtcccct aaggggtaaa acagccgttt
     4441 accgcagatc ctctcattgt gcttttctag aataacaccc ttctagggga ggcgggtggg
     4501 ggagggaggg atcataaccc cttctgtgcc ttgggatgcc ggagctgggg gacctggagg
     4561 cccatcagcc ggagccacgt gaaaggtact gaagaaagct gagacccggc tgtgaggagc
     4621 gcctcagcgg tgaggtggtt tagggataaa tgtttctgga accctgtggt cccccataat
     4681 gttgatagaa tatcatatgc actgggagtt aaatatattt aatttaatga tcattatata
     4741 tgtgggggtt aatatgttgt ttttctgtcc ctttaaagtc tttacatgta attgtagctg
     4801 tataatcgtt atttttcttt tgcatcttaa gtcttagaaa ttaagatatt ccatcgtgag
     4861 gatgagagag gtcctcagtg tgtttttggt ctggttgtag ggaaggactc aagtcctgga
     4921 atgtcctcca ctggtctact gagttgcagt cacactgttc caatggatta tttgctttcg
     4981 gttgtaaatt taattgtaca tatggttgat ttattatttt taaaaataca gactaactga
     5041 tgtaatgttt atgtataagt tgcaccaaaa atcaaggaca aaaataagtg tgtttgtttt
     5101 tacaggtgtg aaagtcacag cttgtaaata agtgttgtat gtattaaacc ttttccagtt
     5161 ctccaaagcg atgtattttt gtacacttga aatagagtac tcttaattta ctgggcaaat
     5221 gtgcttggaa ttgaacttga caagattagc tcaagcagat agagtcgggt ccagcagtgg
     5281 gtggccctcg tgtgaatccc cgtggatgtg caagttgtgg agagaaggag caccgggttc
     5341 ctgcccagca ctgtgcttgc gggaggcggt ggggcatggg aggaaggagg cacagaccgg
     5401 ggaaatatga cagccgtcat ttccagtatt ctctgtgttg tcttttagct cattcaataa
     5461 ataaaggtgg tgtgattttt ttttcctcct gtctttttca tttgtagaaa ctggagacgt
     5521 gtaaagaaga taaataattg tgtaattaaa ctttccagaa atttatcttc ctcatgtgca
     5581 gtttaacaaa cttggtcaaa ctagttagca aattagaact tcagaatcta atgatagttt
     5641 agggtttcta aaataaggtt ttttattgta aaaattgacg attgccctgc atttctacca
     5701 agtcctgtga ataaagagat gggagatttg attccgtcag aagagactgt aatccgtgtc
     5761 gtcagcctgg gagccttccc cagtgtaatg tagctttctc tcttaccttc tggaagaggg
     5821 aatgtttcat ttattactgt ttgattttct tgtatctggt tctactccca ggatgaaatt
     5881 atccaactac atatatattt agaggaagaa agtgaagggg aaatttaaaa tgtttacggc
     5941 gcttaattgc ctggaaatga aatgaaatca aatttatcag tttttttccc cctaattacc
     6001 caaaagatct tttgcaaact atgttacatg aatgcttctg cctctttaag acaaagaaga
     6061 atgtcaccca aaattgtcat ttttttctta atgttcatca taaaagtcct aaaagagtaa
     6121 ctgtaattgg atgtttattg tttttatcta aagtaaggtg tatgtgtttg agacaagctg
     6181 gttttgttga taaagagatg ttaaataatt gtgaagccag atatgcaatg tgtatctgaa
     6241 aagcaaggaa tttgcagccg ttttacaaat atctgtggaa catgtaaata ctgtcaaatg
     6301 gaaaataaaa taagttataa tttttgtgaa tttcatggga tgtcctatga ttggaaaaat
     6361 tataactctt ctgattctaa tgtggaaatt gttgtattta atctgaaaat gactttacct
     6421 acaacagttc cattgtcagc acagcctagg aaggtcagat cctgtattaa ttactcttag
     6481 tggagatgcc agatatccca tacagaatta gcagagaaaa tacacacagg cttctattca
     6541 aattttcttt agtgcttaaa attaagtttt aaaatgaaat cagacactgc aggtttgtat
     6601 ataaaatgaa aagctatact actttttaca aaagggcaaa ctgggctgat gtaaatgttt
     6661 tactttcaac tgtgttcttt aaaataaatc ctacctggtt tttaaatttt atttttcatg
     6721 aaaatgctcc tttctctaca tttattcatc ctatatacat caggctgtaa gacccccccc
     6781 agtcatcatt aatacaatgt gttgggattc tgtgactgga aaaggtgaca agttggtgac
     6841 tttgacactg caggtattcc attttcatgg tttactatga aaagtcattt ttcatattat
     6901 gtaatatatt gttagattaa aaccattgta ttaagacttt aaaatgtaag cattgtaatt
     6961 ctgaaaatac acattttaag aagaaa
//
