LOCUS       NM_014294               3056 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens translocation associated membrane protein 1 (TRAM1),
            transcript variant 1, mRNA.
ACCESSION   NM_014294 XM_001721007 XM_001721035 XM_001721065
VERSION     NM_014294.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Li,J., Gui,Q., Liang,F.-X., Sall,J., Zhang,Q., Duan,Y.,
            Dhabaria,A., Askenazi,M., Ueberheide,B., Stapleford,K.A. and
            Pagano,M.
  TITLE     The REEP5/TRAM1 complex binds SARS-CoV-2 NSP3 and promotes virus
            replication
  JOURNAL   J Virol 97 (10), e0050723 (2023)
   PUBMED   37768083
  REMARK    GeneRIF: The REEP5/TRAM1 complex binds SARS-CoV-2 NSP3 and promotes
            virus replication.
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Deng,Y., You,L., Lu,Y., Han,S., Wang,J., Vicas,N., Chen,C. and
            Ye,J.
  TITLE     Identification of TRAMs as sphingolipid-binding proteins using a
            photoactivatable and clickable short-chain ceramide analog
  JOURNAL   J Biol Chem 297 (6), 101415 (2021)
   PUBMED   34793833
  REMARK    GeneRIF: Identification of TRAMs as sphingolipid-binding proteins
            using a photoactivatable and clickable short-chain ceramide analog.
REFERENCE   3  (bases 1 to 3056)
  AUTHORS   Dubois,J. and Sczakiel,G.
  TITLE     The human TRAM1 locus expresses circular RNAs
  JOURNAL   Sci Rep 11 (1), 22114 (2021)
   PUBMED   34764360
  REMARK    GeneRIF: The human TRAM1 locus expresses circular RNAs.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3056)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 3056)
  AUTHORS   Tang,Z., Zhang,W., Wan,C., Xu,G., Nie,X., Zhu,X., Xia,N., Zhao,Y.,
            Wang,S., Cui,S. and Wang,C.
  TITLE     TRAM1 protect HepG2 cells from palmitate induced insulin resistance
            through ER stress-JNK pathway
  JOURNAL   Biochem Biophys Res Commun 457 (4), 578-584 (2015)
   PUBMED   25600807
  REMARK    GeneRIF: TRAM1 protect HepG2 cells from palmitate-induced insulin
            resistance. The protection of TRAM1 to HepG2 cells is through
            endoplasmic reticulum stress-JNK pathway.
REFERENCE   6  (bases 1 to 3056)
  AUTHORS   Meacock,S.L., Lecomte,F.J., Crawshaw,S.G. and High,S.
  TITLE     Different transmembrane domains associate with distinct endoplasmic
            reticulum components during membrane integration of a polytopic
            protein
  JOURNAL   Mol Biol Cell 13 (12), 4114-4129 (2002)
   PUBMED   12475939
REFERENCE   7  (bases 1 to 3056)
  AUTHORS   Hegde,R.S., Voigt,S., Rapoport,T.A. and Lingappa,V.R.
  TITLE     TRAM regulates the exposure of nascent secretory proteins to the
            cytosol during translocation into the endoplasmic reticulum
  JOURNAL   Cell 92 (5), 621-631 (1998)
   PUBMED   9506517
REFERENCE   8  (bases 1 to 3056)
  AUTHORS   Do,H., Falcone,D., Lin,J., Andrews,D.W. and Johnson,A.E.
  TITLE     The cotranslational integration of membrane proteins into the
            phospholipid bilayer is a multistep process
  JOURNAL   Cell 85 (3), 369-378 (1996)
   PUBMED   8616892
REFERENCE   9  (bases 1 to 3056)
  AUTHORS   Gorlich,D., Hartmann,E., Prehn,S. and Rapoport,T.A.
  TITLE     A protein of the endoplasmic reticulum involved early in
            polypeptide translocation
  JOURNAL   Nature 357 (6373), 47-52 (1992)
   PUBMED   1315422
REFERENCE   10 (bases 1 to 3056)
  AUTHORS   Walter,P.
  TITLE     Protein translocation. Travelling by TRAM
  JOURNAL   Nature 357 (6373), 22-23 (1992)
   PUBMED   1315419
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB448315.1, BI465346.1,
            BC037738.1, AC120194.6 and AA740941.1.
            
            On Nov 23, 2018 this sequence version replaced NM_014294.5.
            
            Summary: This gene encodes a multi-pass membrane protein that is
            part of the mammalian endoplasmic reticulum. The encoded protein
            influences glycosylation and facilitates the translocation of
            secretory proteins across the endoplasmic reticulum membrane by
            regulating which domains of the nascent polypeptide chain are
            visible to the cytosol during a translocational pause. [provided by
            RefSeq, Oct 2009].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.146865.1,
                                           SRR1660803.30986.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262213.7/ ENSP00000262213.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-43                DB448315.1         44-86
            44-47               BI465346.1         74-77
            48-1940             BC037738.1         3-1895
            1941-2866           AC120194.6         148267-149192       c
            2867-3056           AA740941.1         4-193               c
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q13.3"
     gene            1..3056
                     /gene="TRAM1"
                     /gene_synonym="PNAS8; TRAM; TRAMP"
                     /note="translocation associated membrane protein 1"
                     /db_xref="GeneID:23471"
                     /db_xref="HGNC:HGNC:20568"
                     /db_xref="MIM:605190"
     exon            1..340
                     /gene="TRAM1"
                     /gene_synonym="PNAS8; TRAM; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     CDS             218..1342
                     /gene="TRAM1"
                     /gene_synonym="PNAS8; TRAM; TRAMP"
                     /note="isoform 1 is encoded by transcript variant 1;
                     translocating chain-associating membrane protein;
                     translocation-associating membrane protein 1;
                     translocating chain-associated membrane protein 1"
                     /codon_start=1
                     /product="translocating chain-associated membrane protein
                     1 isoform 1"
                     /protein_id="NP_055109.1"
                     /db_xref="CCDS:CCDS6207.1"
                     /db_xref="GeneID:23471"
                     /db_xref="HGNC:HGNC:20568"
                     /db_xref="MIM:605190"
                     /translation="MAIRKKSTKSPPVLSHEFVLQNHADIVSCVAMVFLLGLMFEITA
                     KASIIFVTLQYNVTLPATEEQATESVSLYYYGIKDLATVFFYMLVAIIIHAVIQEYML
                     DKINRRMHFSKTKHSKFNESGQLSAFYLFACVWGTFILISENYISDPTILWRAYPHNL
                     MTFQMKFFYISQLAYWLHAFPELYFQKTKKEDIPRQLVYIGLYLFHIAGAYLLNLNHL
                     GLVLLVLHYFVEFLFHISRLFYFSNEKYQKGFSLWAVLFVLGRLLTLILSVLTVGFGL
                     ARAENQKLDFSTGNFNVLAVRIAVLASICVTQAFMMWKFINFQLRRWREHSAFQAPAV
                     KKKPTVTKGRSSKKGTENGVNGTLTSNVADSPRNKKEKSS"
     exon            341..404
                     /gene="TRAM1"
                     /gene_synonym="PNAS8; TRAM; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     exon            405..526
                     /gene="TRAM1"
                     /gene_synonym="PNAS8; TRAM; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     exon            527..643
                     /gene="TRAM1"
                     /gene_synonym="PNAS8; TRAM; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     exon            644..702
                     /gene="TRAM1"
                     /gene_synonym="PNAS8; TRAM; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     exon            703..787
                     /gene="TRAM1"
                     /gene_synonym="PNAS8; TRAM; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     exon            788..858
                     /gene="TRAM1"
                     /gene_synonym="PNAS8; TRAM; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     exon            859..963
                     /gene="TRAM1"
                     /gene_synonym="PNAS8; TRAM; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     exon            964..1107
                     /gene="TRAM1"
                     /gene_synonym="PNAS8; TRAM; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1268
                     /gene="TRAM1"
                     /gene_synonym="PNAS8; TRAM; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1269..3056
                     /gene="TRAM1"
                     /gene_synonym="PNAS8; TRAM; TRAMP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1429..1434
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRAM1"
                     /gene_synonym="PNAS8; TRAM; TRAMP"
                     /note="hexamer: AATACA"
     polyA_site      1449
                     /gene="TRAM1"
                     /gene_synonym="PNAS8; TRAM; TRAMP"
     regulatory      2810..2815
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRAM1"
                     /gene_synonym="PNAS8; TRAM; TRAMP"
                     /note="hexamer: AATAAA"
     polyA_site      2829
                     /gene="TRAM1"
                     /gene_synonym="PNAS8; TRAM; TRAMP"
                     /note="major polyA site"
     regulatory      3026..3031
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TRAM1"
                     /gene_synonym="PNAS8; TRAM; TRAMP"
                     /note="hexamer: AATAAA"
     polyA_site      3056
                     /gene="TRAM1"
                     /gene_synonym="PNAS8; TRAM; TRAMP"
ORIGIN      
        1 atgctcgccc ccgctgcggg ctagctgttg tgtttttttt tttcccccgg gcggcccggc
       61 ggctgcgtac tggctgtggg atgggaagtg aagccccagc gagcggctgc agcggggccg
      121 tgaggagcag ccagcgggag gcggcggcga gtcggtgagc agctgggaag agcagaaccg
      181 gggcggagca cctgcaggcg cgggcggcgg ccccaccatg gcgattcgca agaaaagcac
      241 caagagcccc ccagtgctga gccacgaatt cgtcctgcag aatcacgcgg acatcgtctc
      301 ctgtgtggcg atggtcttcc tgctggggct catgtttgag ataacggcaa aagcttctat
      361 catttttgtt actcttcagt acaatgtcac cctcccagca acagaagaac aagctactga
      421 atcagtgtcc ctttattact atggcatcaa agatttggct actgttttct tctacatgct
      481 agtggcgata attattcatg ccgtaattca agagtatatg ttggataaaa ttaacaggcg
      541 aatgcacttc tccaaaacaa aacacagcaa gtttaatgaa tctggtcagc ttagtgcgtt
      601 ctaccttttt gcctgtgttt ggggcacatt cattctcatc tctgaaaact acatctcaga
      661 cccaactatc ttatggaggg cttatcccca taacctgatg acatttcaaa tgaagttttt
      721 ctacatatca cagctggctt actggcttca tgcttttcct gaactctact tccagaaaac
      781 caaaaaagaa gatattcctc gtcagcttgt ctacattggt ctttacctct tccacattgc
      841 tggagcttac cttttgaact tgaatcatct aggacttgtt cttctggtgc tacattattt
      901 tgttgaattt cttttccaca tttcccgcct gttttatttt agcaatgaaa agtatcagaa
      961 aggattttct ctgtgggcag ttctttttgt tttgggaaga cttctgactt taattctttc
     1021 agtactgact gttggttttg gccttgcaag agcagaaaat cagaagctgg atttcagtac
     1081 tggaaacttc aatgtgttag ctgttagaat cgctgttctg gcatccattt gcgttactca
     1141 ggcatttatg atgtggaagt tcattaattt tcagcttcga aggtggaggg aacattctgc
     1201 ttttcaggca ccagctgtga agaagaaacc aacagtaact aaaggcagat cttctaaaaa
     1261 aggaacagaa aatggtgtga atggaacatt aacttcaaat gtagcagact ctccccggaa
     1321 taaaaaagag aaatcttcat aatgaattat aaactaattg attaatgtcc ccaaagaaat
     1381 ctgctttcta ctatatcttt cagcattaga gatttttctg ttcttgaaaa tacagtctgt
     1441 gctctttgat ttttgctatt gtacggtttc atgcattttt ttaaagggca tttgagggga
     1501 ggattattgc tatgaatgaa aaaaatattt tagcttagac taagctacct gccttcaaaa
     1561 tagtttaggg accaccacca tattttattt tgtttttatt tttgaacatt tttctaatga
     1621 tttggagaga aaactattta caaaaattcc acatatcagt gatacaattt cttgctgtca
     1681 ccaatttttt ataatagcag agtggcctgt tctaagaagg ccatattttt taagttatct
     1741 ttcagggtaa catggaaata ctataaagtt ggatgtcaaa ctttaatatg ttttcagtgt
     1801 tctctaattt tttggaattt ttgtagactt tacacctgga aaaaaagatt tgtaaaatca
     1861 ccggaacaat tgtgtgcttt attttatagg tagtggttat tagtattaca tccccatttt
     1921 aaaaacaaaa acataataat cgttacaaca cgtggagttt tactaacata catattaaat
     1981 caaagtatat tcttaaaagt acttgtgaag taaaatcttt cttgtgcatt ttcaatactt
     2041 gtaaactgga aatcagaaaa tatttactat gaacaggaaa atctgacata tagccctttt
     2101 tgatatgttt attaataatg attcttaatg gggctcataa taagtttaat atgcacagca
     2161 tcttagaaaa gtttaacctg caaacacttt taaaacataa tgcctacttg atttatatct
     2221 ataaaaagac tgacaggtaa ttatatttgg aaaacattta atgcactaac tttaaagaaa
     2281 ttgaaaattc aggtggataa atagtcttac aaaagacaat gtgctttatg ttatacctat
     2341 agctttggtc ccatctttaa ttgagaaaca tttatctgta taaaacatat ttttggataa
     2401 atatatatat atatatttgt atctctacag aaaggctcta aaaagcattt gaggaaaata
     2461 tttggttccc ttttctataa tcatccttta agattcttat agctacattt ggtttattca
     2521 tcatatttac agtatatata ttgttctttt cagtgttcac atcttgttcc ccatttctca
     2581 cttgtgtcac cagctgtttg tgccattttt agtgtaaaag ttgcagacct attagatctg
     2641 cagtttaagt tgccatgctg ctaggaaatt gtcctttttc tttctagctg ttaacctact
     2701 tcctggaaaa agtagtagct ctctgtagca ttatggagtt tcagtggaac caaatttttg
     2761 ccattaaaaa ctggcattat actgaactat acattgagaa atcaatcaaa ataaaaattt
     2821 ttactttcac aagttgtatc ctggatgttt ctgtcattgt tggtgtttag gctattttgg
     2881 tatataacct cattaaaatg taccatattt aaaacacttc atagacattc agaataaccc
     2941 ttttcaaaat tgtgttctgc aaataaacag atttgttcca cagaatattg gtgaggacta
     3001 ctggggggat agatggtcat aaccaaataa atctgggaat cactggatta aactaa
//
