LOCUS       NM_012112               3473 bp    mRNA    linear   PRI 01-AUG-2024
DEFINITION  Homo sapiens TPX2 microtubule nucleation factor (TPX2), mRNA.
ACCESSION   NM_012112
VERSION     NM_012112.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Yu,J., Wang,W., Jiang,Z. and Liu,H.
  TITLE     TPX2 upregulates MMP13 to promote the progression of
            lipopolysaccharide-induced osteoarthritis
  JOURNAL   PeerJ 12, e17032 (2024)
   PUBMED   38770093
  REMARK    GeneRIF: TPX2 upregulates MMP13 to promote the progression of
            lipopolysaccharide-induced osteoarthritis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Wang,L., Zhang,H., Li,Y. and Li,L.
  TITLE     TPX2 influences the regulation of macrophage polarization via the
            NF-kappaB pathway in lung adenocarcinoma
  JOURNAL   Life Sci 340, 122437 (2024)
   PUBMED   38266813
  REMARK    GeneRIF: TPX2 influences the regulation of macrophage polarization
            via the NF-kappaB pathway in lung adenocarcinoma.
REFERENCE   3  (bases 1 to 3473)
  AUTHORS   Zhu,M., Wang,X., Zhang,Q., Xie,C., Wang,T., Shen,K., Zhang,L. and
            Zhou,X.
  TITLE     Integrative analysis confirms TPX2 as a novel biomarker for
            clinical implication, tumor microenvironment, and immunotherapy
            response across human solid tumors
  JOURNAL   Aging (Albany NY) 16 (3), 2563-2590 (2024)
   PUBMED   38315450
  REMARK    GeneRIF: Integrative analysis confirms TPX2 as a novel biomarker
            for clinical implication, tumor microenvironment, and immunotherapy
            response across human solid tumors.
REFERENCE   4  (bases 1 to 3473)
  AUTHORS   Liu,N., Jiang,Y., Chen,S., Pan,F., Tang,Y. and Tan,X.
  TITLE     miRNA-27b-3p/TPX2 Axis Regulates Clear Cell Renal Cell Carcinoma
            Cell Proliferation, Invasion and Migration
  JOURNAL   Crit Rev Eukaryot Gene Expr 34 (1), 27-39 (2024)
   PUBMED   37824390
  REMARK    GeneRIF: miRNA-27b-3p/TPX2 Axis Regulates Clear Cell Renal Cell
            Carcinoma Cell Proliferation, Invasion and Migration.
REFERENCE   5  (bases 1 to 3473)
  AUTHORS   Qi,X., Liu,Y., Peng,Y., Fu,Y., Fu,Y., Yin,L. and Li,X.
  TITLE     UHRF1 promotes spindle assembly and chromosome congression by
            catalyzing EG5 polyubiquitination
  JOURNAL   J Cell Biol 222 (11) (2023)
   PUBMED   37728657
REFERENCE   6  (bases 1 to 3473)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
REFERENCE   7  (bases 1 to 3473)
  AUTHORS   Wittmann,T., Wilm,M., Karsenti,E. and Vernos,I.
  TITLE     TPX2, A novel xenopus MAP involved in spindle pole organization
  JOURNAL   J Cell Biol 149 (7), 1405-1418 (2000)
   PUBMED   10871281
REFERENCE   8  (bases 1 to 3473)
  AUTHORS   Manda,R., Kohno,T., Matsuno,Y., Takenoshita,S., Kuwano,H. and
            Yokota,J.
  TITLE     Identification of genes (SPON2 and C20orf2) differentially
            expressed between cancerous and noncancerous lung cells by mRNA
            differential display
  JOURNAL   Genomics 61 (1), 5-14 (1999)
   PUBMED   10512675
REFERENCE   9  (bases 1 to 3473)
  AUTHORS   Zhang,Y., Heidebrecht,H., Rott,A., Schlegelberger,B. and
            Parwaresch,R.
  TITLE     Assignment of human proliferation associated p100 gene (C20orf1) to
            human chromosome band 20q11.2 by in situ hybridization
  JOURNAL   Cytogenet Cell Genet 84 (3-4), 182-183 (1999)
   PUBMED   10393424
REFERENCE   10 (bases 1 to 3473)
  AUTHORS   Heidebrecht,H.J., Buck,F., Steinmann,J., Sprenger,R., Wacker,H.H.
            and Parwaresch,R.
  TITLE     p100: a novel proliferation-associated nuclear protein specifically
            restricted to cell cycle phases S, G2, and M
  JOURNAL   Blood 90 (1), 226-233 (1997)
   PUBMED   9207457
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB960160.1, AF098158.2 and BC020207.1.
            
            On Nov 22, 2018 this sequence version replaced NM_012112.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.191620.1,
                                           SRR1803615.244086.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000300403.11/ ENSP00000300403.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                CB960160.1         46-83
            39-52               AF098158.2         1-14
            53-3473             BC020207.1         1-3421
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q11.21"
     gene            1..3473
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /note="TPX2 microtubule nucleation factor"
                     /db_xref="GeneID:22974"
                     /db_xref="HGNC:HGNC:1249"
                     /db_xref="MIM:605917"
     exon            1..332
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            333..439
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            440..615
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     CDS             510..2753
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /note="preferentially expressed in colorectal cancer;
                     differentially expressed in lung cells; hepatocellular
                     carcinoma-associated antigen 519; restricted expression
                     proliferation associated protein 100; protein fls353;
                     differentially expressed in cancerous and non-cancerous
                     lung cells 2; TPX2, microtubule-associated, homolog;
                     hepatocellular carcinoma-associated antigen 90; TPX2,
                     microtubule-associated protein homolog; differentially
                     expressed in cancerous and noncancerous lung cells 2"
                     /codon_start=1
                     /product="targeting protein for Xklp2"
                     /protein_id="NP_036244.2"
                     /db_xref="CCDS:CCDS13190.1"
                     /db_xref="GeneID:22974"
                     /db_xref="HGNC:HGNC:1249"
                     /db_xref="MIM:605917"
                     /translation="MSQVKSSYSYDAPSDFINFSSLDDEGDTQNIDSWFEEKANLENK
                     LLGKNGTGGLFQGKTPLRKANLQQAIVTPLKPVDNTYYKEAEKENLVEQSIPSNACSS
                     LEVEAAISRKTPAQPQRRSLRLSAQKDLEQKEKHHVKMKAKRCATPVIIDEILPSKKM
                     KVSNNKKKPEEEGSAHQDTAEKNASSPEKAKGRHTVPCMPPAKQKFLKSTEEQELEKS
                     MKMQQEVVEMRKKNEEFKKLALAGIGQPVKKSVSQVTKSVDFHFRTDERIKQHPKNQE
                     EYKEVNFTSELRKHPSSPARVTKGCTIVKPFNLSQGKKRTFDETVSTYVPLAQQVEDF
                     HKRTPNRYHLRSKKDDINLLPSKSSVTKICRDPQTPVLQTKHRARAVTCKSTAELEAE
                     ELEKLQQYKFKARELDPRILEGGPILPKKPPVKPPTEPIGFDLEIEKRIQERESKKKT
                     EDEHFEFHSRPCPTKILEDVVGVPEKKVLPITVPKSPAFALKNRIRMPTKEDEEEDEP
                     VVIKAQPVPHYGVPFKPQIPEARTVEICPFSFDSRDKERQLQKEKKIKELQKGEVPKF
                     KALPLPHFDTINLPEKKVKNVTQIEPFCLETDRRGALKAQTWKHQLEEELRQQKEAAC
                     FKARPNTVISQEPFVPKKEKKSVAEGLSGSLVQEPFQLATEKRAKERQELEKRMAEVE
                     AQKAQQLEEARLQEEEQKKEELARLRRELVHKANPIRKYQGLEIKSSDQPLTVPVSPK
                     FSTRFHC"
     exon            616..738
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            739..865
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            866..994
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1117
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1239
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1391
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1563
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            1564..1705
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            1706..1922
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            1923..2018
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            2019..2195
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            2196..2342
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            2343..2454
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            2455..2642
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     exon            2643..3473
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3146..3151
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /note="hexamer: AATAAA"
     polyA_site      3167
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /note="major polyA site"
     regulatory      3449..3454
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /note="hexamer: AATAAA"
     polyA_site      3473
                     /gene="TPX2"
                     /gene_synonym="C20orf1; C20orf2; DIL-2; DIL2; FLS353;
                     GD:C20orf1; HCA519; HCTP4; p100; REPP86"
                     /note="major polyA site"
ORIGIN      
        1 gaatgagtcc cgcggcgggt tggctcgcgc ttcgttgtca gatctgaggc gaggctaggt
       61 gagccgtggg aagaaaagag ggagcagcta gggcgcgggt ctccctcctc ccggagtttg
      121 gaacggctga agttcacctt ccagccccta gcgccgttcg cgccgctagg cctggcttct
      181 gaggcggttg cggtgctcgg tcgccgccta ggcggggcag ggtgcgagca ggggcttcgg
      241 gccacgcttc tcttggcgac aggattttgc tgtgaagtcc gtccgggaaa cggaggaaaa
      301 aaagagttgc gggaggctgt cggctaataa cggttcttga tacatatttg ccagacttca
      361 agatttcaga aaaggggtga aagagaagat tgcaactttg agtcagacct gtaggcctga
      421 tagactgatt aaaccacaga aggtgacctg ctgagaaaag tggtacaaat actgggaaaa
      481 acctgctctt ctgcgttaag tgggagacaa tgtcacaagt taaaagctct tattcctatg
      541 atgccccctc ggatttcatc aatttttcat ccttggatga tgaaggagat actcaaaaca
      601 tagattcatg gtttgaggag aaggccaatt tggagaataa gttactgggg aagaatggaa
      661 ctggagggct ttttcagggc aaaactcctt tgagaaaggc taatcttcag caagctattg
      721 tcacaccttt gaaaccagtt gacaacactt actacaaaga ggcagaaaaa gaaaatcttg
      781 tggaacaatc cattccgtca aatgcttgtt cttccctgga agttgaggca gccatatcaa
      841 gaaaaactcc agcccagcct cagagaagat ctcttaggct ttctgctcag aaggatttgg
      901 aacagaaaga aaagcatcat gtaaaaatga aagccaagag atgtgccact cctgtaatca
      961 tcgatgaaat tctaccctct aagaaaatga aagtttctaa caacaaaaag aagccagagg
     1021 aagaaggcag tgctcatcaa gatactgctg aaaagaatgc atcttcccca gagaaagcca
     1081 agggtagaca tactgtgcct tgtatgccac ctgcaaagca gaagtttcta aaaagtactg
     1141 aggagcaaga gctggagaag agtatgaaaa tgcagcaaga ggtggtggag atgcggaaaa
     1201 agaatgaaga attcaagaaa cttgctctgg ctggaatagg gcaacctgtg aagaaatcag
     1261 tgagccaggt caccaaatca gttgacttcc acttccgcac agatgagcga atcaaacaac
     1321 atcctaagaa ccaggaggaa tataaggaag tgaactttac atctgaacta cgaaagcatc
     1381 cttcatctcc tgcccgagtg actaagggat gtaccattgt taagcctttc aacctgtccc
     1441 aaggaaagaa aagaacattt gatgaaacag tttctacata tgtgcccctt gcacagcaag
     1501 ttgaagactt ccataaacga acccctaaca gatatcattt gaggagcaag aaggatgata
     1561 ttaacctgtt accctccaaa tcttctgtga ccaagatttg cagagaccca cagactcctg
     1621 tactgcaaac caaacaccgt gcacgggctg tgacctgcaa aagtacagca gagctggagg
     1681 ctgaggagct cgagaaattg caacaataca aattcaaagc acgtgaactt gatcccagaa
     1741 tacttgaagg tgggcccatc ttgcccaaga aaccacctgt gaaaccaccc accgagccta
     1801 ttggctttga tttggaaatt gagaaaagaa tccaggagcg agaatcaaag aagaaaacag
     1861 aggatgaaca ctttgaattt cattccagac cttgccctac taagattttg gaagatgttg
     1921 tgggtgttcc tgaaaagaag gtacttccaa tcaccgtccc caagtcacca gcctttgcat
     1981 tgaagaacag aattcgaatg cccaccaaag aagatgagga agaggacgaa ccggtagtga
     2041 taaaagctca acctgtgcca cattatgggg tgccttttaa gccccaaatc ccagaggcaa
     2101 gaactgtgga aatatgccct ttctcgtttg attctcgaga caaagaacgt cagttacaga
     2161 aggagaagaa aataaaagaa ctgcagaaag gggaggtgcc caagttcaag gcacttccct
     2221 tgcctcattt tgacaccatt aacctgccag agaagaaggt aaagaatgtg acccagattg
     2281 aacctttctg cttggagact gacagaagag gtgctctgaa ggcacagact tggaagcacc
     2341 agctggaaga agaactgaga cagcagaaag aagcagcttg tttcaaggct cgtccaaaca
     2401 ccgtcatctc tcaggagccc tttgttccca agaaagagaa gaaatcagtt gctgagggcc
     2461 tttctggttc tctagttcag gaaccttttc agctggctac tgagaagaga gccaaagagc
     2521 ggcaggagct ggagaagaga atggctgagg tagaagccca gaaagcccag cagttggagg
     2581 aggccagact acaggaggaa gagcagaaaa aagaggagct ggccaggcta cggagagaac
     2641 tggtgcataa ggcaaatcca atacgcaagt accagggtct ggagataaag tcaagtgacc
     2701 agcctctgac tgtgcctgta tctcccaaat tctccactcg attccactgc taaactcagc
     2761 tgtgagctgc ggataccgcc cggcaatggg acctgctctt aacctcaaac ctaggaccgt
     2821 cttgctttgt cattgggcat ggagagaacc catttctcca gacttttacc tacccgtgcc
     2881 tgagaaagca tacttgacaa ctgtggactc cagttttgtt gagaattgtt ttcttacatt
     2941 actaaggcta ataatgagat gtaactcatg aatgtctcga ttagactcca tgtagttact
     3001 tcctttaaac catcagccgg ccttttatat gggtcttcac tctgactaga atttagtctc
     3061 tgtgtcagca cagtgtaatc tctattgcta ttgcccctta cgactctcac cctctcccca
     3121 ctttttttaa aaattttaac cagaaaataa agatagttaa atcctaagat agagattaag
     3181 tcatggttta aatgaggaac aatcagtaaa tcagattctg tcctcttctc tgcataccgt
     3241 gaatttatag ttaaggatcc ctttgctgtg agggtagaaa acctcaccaa ctgcaccagt
     3301 gaggaagaag actgcgtgga ttcatgggga gcctcacagc agccacgcag caggctctgg
     3361 gtggggctgc cgttaaggca cgttctttcc ttactggtgc tgataacaac agggaaccgt
     3421 gcagtgtgca ttttaagacc tggcctggaa taaatacgtt ttgtctttcc ctc
//
