LOCUS       NM_001136053            3752 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens transmembrane protein adipocyte associated 1 (TPRA1),
            transcript variant 1, mRNA.
ACCESSION   NM_001136053
VERSION     NM_001136053.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Singh,J., Wen,X. and Scales,S.J.
  TITLE     The Orphan G Protein-coupled Receptor Gpr175 (Tpra40) Enhances
            Hedgehog Signaling by Modulating cAMP Levels
  JOURNAL   J Biol Chem 290 (49), 29663-29675 (2015)
   PUBMED   26451044
  REMARK    GeneRIF: Gpr175 is a novel positive regulator of the Hh signaling
            pathway
REFERENCE   3  (bases 1 to 3752)
  AUTHORS   Shen,C., Huo,L.R., Zhao,X.L., Wang,P.R. and Zhong,N.
  TITLE     Novel interactive partners of neuroligin 3: new aspects for
            pathogenesis of autism
  JOURNAL   J Mol Neurosci 56 (1), 89-101 (2015)
   PUBMED   25464930
REFERENCE   4  (bases 1 to 3752)
  AUTHORS   Ma,J., Dempsey,A.A., Stamatiou,D., Marshall,K.W. and Liew,C.C.
  TITLE     Identifying leukocyte gene expression patterns associated with
            plasma lipid levels in human subjects
  JOURNAL   Atherosclerosis 191 (1), 63-72 (2007)
   PUBMED   16806233
REFERENCE   5  (bases 1 to 3752)
  AUTHORS   Yang,H., Egan,J.M., Rodgers,B.D., Bernier,M. and
            Montrose-Rafizadeh,C.
  TITLE     Differential expression of a novel seven transmembrane domain
            protein in epididymal fat from aged and diabetic mice
  JOURNAL   Endocrinology 140 (6), 2859-2867 (1999)
   PUBMED   10342878
REFERENCE   6  (bases 1 to 3752)
  AUTHORS   Bussow,K., Cahill,D., Nietfeld,W., Bancroft,D., Scherzinger,E.,
            Lehrach,H. and Walter,G.
  TITLE     A method for global protein expression and antibody screening on
            high-density filters of an arrayed cDNA library
  JOURNAL   Nucleic Acids Res 26 (21), 5007-5008 (1998)
   PUBMED   9776767
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            HY066741.1, BC050711.2, AC084035.24 and BM670609.1.
            
            On May 17, 2019 this sequence version replaced NM_001136053.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.4316174.1,
                                           SRR18074969.3498320.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000355552.8/ ENSP00000347748.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-80                HY066741.1         28-107
            81-1912             BC050711.2         1-1832
            1913-3721           AC084035.24        20693-22501
            3722-3752           BM670609.1         17-47               c
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q21.3"
     gene            1..3752
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
                     /note="transmembrane protein adipocyte associated 1"
                     /db_xref="GeneID:131601"
                     /db_xref="HGNC:HGNC:30413"
                     /db_xref="MIM:608336"
     exon            1..324
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
                     /inference="alignment:Splign:2.1.0"
     exon            325..466
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
                     /inference="alignment:Splign:2.1.0"
     CDS             342..1463
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
                     /note="isoform 1 is encoded by transcript variant 1;
                     transmembrane protein 227; transmembrane protein,
                     adipocyte asscociated 1; seven transmembrane domain orphan
                     receptor; G protein-coupled receptor 175; transmembrane
                     domain protein regulated in adipocytes 40 kDa; integral
                     membrane protein GPR175"
                     /codon_start=1
                     /product="transmembrane protein adipocyte-associated 1
                     isoform 1"
                     /protein_id="NP_001129525.1"
                     /db_xref="CCDS:CCDS3042.1"
                     /db_xref="GeneID:131601"
                     /db_xref="HGNC:HGNC:30413"
                     /db_xref="MIM:608336"
                     /translation="MDTLEEVTWANGSTALPPPLAPNISVPHRCLLLLYEDIGTSRVR
                     YWDLLLLIPNVLFLIFLLWKLPSARAKIRITSSPIFITFYILVFVVALVGIARAVVSM
                     TVSTSNAATVADKILWEITRFFLLAIELSVIILGLAFGHLESKSSIKRVLAITTVLSL
                     AYSVTQGTLEILYPDAHLSAEDFNIYGHGGRQFWLVSSCFFFLVYSLVVILPKTPLKE
                     RISLPSRRSFYVYAGILALLNLLQGLGSVLLCFDIIEGLCCVDATTFLYFSFFAPLIY
                     VAFLRGFFGSEPKILFSYKCQVDETEEPDVHLPQPYAVARREGLEAAGAAGASAASYS
                     STQFDSAGGVAYLDDIASMPCHTGSINSTDSERWKAINA"
     exon            467..599
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
                     /inference="alignment:Splign:2.1.0"
     exon            600..686
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
                     /inference="alignment:Splign:2.1.0"
     exon            687..759
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
                     /inference="alignment:Splign:2.1.0"
     exon            760..839
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
                     /inference="alignment:Splign:2.1.0"
     exon            840..950
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1011
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1114
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1195
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..3752
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1893..1898
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
                     /note="hexamer: AATAAA"
     polyA_site      1912
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
                     /note="major polyA site"
     polyA_site      1942
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
     regulatory      2231..2236
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
                     /note="hexamer: ATTAAA"
     polyA_site      2252
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
     regulatory      3639..3644
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
                     /note="hexamer: AATATA"
     polyA_site      3665
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
     regulatory      3705..3710
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
                     /note="hexamer: AATAAA"
     polyA_site      3730
                     /gene="TPRA1"
                     /gene_synonym="GPR175; TMEM227; TPRA40"
ORIGIN      
        1 gggactgtgg ccgttttcgc aatttgggct ggacgaaaaa gacggtcttg ctttcccggt
       61 cgccgctgtc gggaagggct gcagggtgtc cgcgagaccc gcggcccggc gagctgaccc
      121 cgcctcgcct ttcctgccta gccctcattc cacggagccg gtcgcgcccg gtccttgcgc
      181 gacgcttccc ggcccaggcc gcctggtctg gcgctggagg ccggagtccc gcggcctgtg
      241 ctggatccgc gcacacccag tggcggcgga tgggcggccg gggcggccgg ggcggccggt
      301 cctgagcgcg gcccgggctg tcagggctgg ctgctggcgg gatggacacc ctggaggagg
      361 tgacttgggc caatgggagc acagcgctac ccccacccct ggcaccaaac atcagtgtgc
      421 ctcatcgctg cctgctgctg ctctacgaag acattggcac ctccagggtc cggtactggg
      481 acctcttgct gctcatcccc aatgtgctct tcctcatctt cctgctctgg aagcttccat
      541 ctgctcgggc gaagatccgc atcacctcca gccccatttt tatcaccttc tacatcctgg
      601 tgtttgtggt ggcgctggtg ggcattgccc gggccgtggt atccatgacg gtgagcacct
      661 cgaacgctgc aactgttgct gataagatcc tgtgggagat cacccgcttc ttcctgctgg
      721 ccatcgagct gagtgtgatc atcctgggcc tggcctttgg ccacctggag agtaagtcca
      781 gcatcaagcg ggtgctggcc atcaccacag tgctgtccct ggcctactct gtcacccagg
      841 ggaccctgga gatcctgtac cctgatgccc atctctcagc tgaggacttt aatatctatg
      901 gccatggggg ccgccagttc tggctggtca gctcctgctt cttcttcctg gtctactctc
      961 tggtggtcat ccttcccaag accccgctga aggagcgcat ctccctgcct tctcggagga
     1021 gcttctacgt gtatgcgggc atcctggcac tgctcaacct actgcagggg ctggggagtg
     1081 tgctgctgtg cttcgacatc atcgaggggc tctgctgtgt agatgccaca accttcctgt
     1141 acttcagctt cttcgctccg ctcatctacg tggctttcct ccggggcttc ttcggctcgg
     1201 agcccaagat cctcttctcc tacaaatgcc aagtggacga gacagaggag ccagatgtac
     1261 acctacccca gccctacgct gtggcccggc gggagggcct ggaggctgca ggggctgctg
     1321 gggcctcagc tgccagctac tcgagcacgc agttcgactc tgccggcggg gtggcctacc
     1381 tggatgacat cgcttccatg ccctgccaca ctggcagcat caacagcaca gacagcgagc
     1441 gctggaaggc catcaatgcc tgagggcagc tgccagggcc tgtggaggac aggccagaga
     1501 ggaggccagc aggcccagag tccccagggg aggaggacca ggtcaaggga cgttctgtgg
     1561 gcagtagccc tgtgtggccc tgttcccacc atgagtctgg aggccccacc tccctggggc
     1621 tcccaatccc ctttgccatc tctgctctca ctggggaccc tcctcccctt cccacctgct
     1681 ctcatactgc tcagtgacat ggcccaggct ttccttccag ggccatgctt ggcaaggttg
     1741 gctgagggca ccctccttct ctgcaccctt ggcacgaggg cagggctggc tctcccaatg
     1801 cctccatccc atccccatgg tgctttggcc tcctcaaagc atccaccatg gtggatggac
     1861 tgaagtgtgt atattttctt gatctatttt ttaataaaaa ggaaaaggag cagaaggtgc
     1921 taatgtggag gttttgcagg aatactgggg ctggttcctt tcaggagcct gagggctggg
     1981 tgaggggctg ggaggctcag ggggcctctg ctcaaccaag gagcagtccc cagtgcccaa
     2041 gccctgggct ggctgctggg gaggtgaaga gaaacaggat cccagctgcc gctgcccctg
     2101 cccctgggcc catctgagta ggggggacag acatggagag agaggagaac tatgttttgt
     2161 gagccagccc ctctcccttt gtcagacaca cactgttgtc ttgggcagcc ggttctctct
     2221 gctcagctcc attaaaacgc agaatggact tactattgcc atctgtaaaa tgcggagaag
     2281 acccatcctg cagggccttt tgtaaggatt ggaaaacact aagcacgagt ggctggcgta
     2341 cagtaatgaa cgataaggat agctcttatt tttagcctca agaatagtgc ttggcacata
     2401 gtaggccccc agcagacagg actcggggac tgatgtttgt cataaggatg ggtgtggttg
     2461 gctgaaaatg ggagtaaatc agagcaaagc actgggatca taaggcatga gtaggggaga
     2521 ggggcccact tgtccctgcc actgcccttg tacctgagtt cggctttgag ctttttggca
     2581 gccagagtga aagggagttg ctgccagtta tgtaatcccc agagttggag aggaggagcc
     2641 catccaggac acagccaact gcatgcttca gagcctgggg aggtctcagc cctggctgtg
     2701 gggaccctga ggatcaggct gttgcccgcc tccgcgatca gccttttttt tttagggggg
     2761 aatggggtgc cgagggcaca agctccaaca cagctcatgt ctgtggggcc aatgtgtcag
     2821 gctccctggg agggcctgga gggttgatag cggacagggc caacagtacg gccagtgagc
     2881 tggactcctg ggctgcaggg ggtactgcag ggtgcccctg aatggcatac tttatcgggg
     2941 cctctcagct catgtaaaag cctggtaatc agggaaggca agctcacggc attctgagtg
     3001 agcggggtaa gggttggggc cagcagggat ttgaccatca gatccacatg cagtccaaga
     3061 tggcacagtg tcagccgaag tttgctggct gcagtgaatg agcagagagg catcaacaga
     3121 agaaaagaga ccatttttat aatttttaag tttgtttttt aaaaataggt catatatgta
     3181 catggcaaga aaaaaaaagg ttcatgcagt acaggagggt atttggtgaa cgataaatct
     3241 ccctgtgtgg cactgaattc ctctgtcccc ctcctgaagc acccagggtc tccataacct
     3301 tccagagcca tcctgtgttc acaccaacgg atgtgtatat catccctctt tctgtaatgc
     3361 taatgtcctg tagctcactc caatggatct tggagatcat tccatgtcaa gtggaggact
     3421 gcttcacccc tgcagctaca tatatatgga tatatggcaa tttataaacc acttcccttt
     3481 tgtttctgaa aaattttgca acaacaaaca aggcaacatc aacaatcctt ataaatttgc
     3541 ttttggtaac tcatgcaagt agaattgctg gagcacacag tcttaattag ttttaattac
     3601 tcttattagt cttctgcaga gatcaatagt tgtcctccaa tatatgttgt ttttctccat
     3661 tggaagagaa tccctgattt ttcgctagat acacagctgt ctagaataaa gactgcattt
     3721 tctagccttc cttgcaggtg ggttctggcc aa
//
