LOCUS       NM_003291               5445 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens tripeptidyl peptidase 2 (TPP2), transcript variant 1,
            mRNA.
ACCESSION   NM_003291
VERSION     NM_003291.4
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Liao,C.L., Hu,R.C., Liao,M.S., Chen,Y.J., Chen,Y.P., Hsieh,H.H.,
            Tai,C.H., Chou,T.C., Chu,C.Y., Chen,Y.J., Lo,L.C. and Lin,J.J.
  TITLE     Unveiling a novel serpinB2-tripeptidyl peptidase II signaling axis
            during senescence
  JOURNAL   J Cell Sci 135 (10) (2022)
   PUBMED   35466366
  REMARK    GeneRIF: Unveiling a novel serpinB2-tripeptidyl peptidase II
            signaling axis during senescence.
REFERENCE   2  (bases 1 to 5445)
  AUTHORS   Atallah,I., Quinodoz,M., Campos-Xavier,B., Peter,V.G., Fouriki,A.,
            Bonvin,C., Bottani,A., Kumps,C., Angelini,F., Bellutti Enders,F.,
            Christen-Zaech,S., Rizzi,M., Renella,R., Beck-Popovic,M.,
            Poloni,C., Frossard,V., Blouin,J.L., Rivolta,C., Riccio,O.,
            Candotti,F., Hofer,M., Unger,S. and Superti-Furga,A.
  TITLE     Immune deficiency, autoimmune disease and intellectual disability:
            A pleiotropic disorder caused by biallelic variants in the TPP2
            gene
  JOURNAL   Clin Genet 99 (6), 780-788 (2021)
   PUBMED   33586135
  REMARK    GeneRIF: Immune deficiency, autoimmune disease and intellectual
            disability: A pleiotropic disorder caused by biallelic variants in
            the TPP2 gene.
REFERENCE   3  (bases 1 to 5445)
  AUTHORS   Lindas,A.C. and Tomkinson,B.
  TITLE     Characterization of the promoter of the gene encoding human
            tripeptidyl-peptidase II and identification of upstream silencer
            elements
  JOURNAL   Gene 393 (1-2), 62-69 (2007)
   PUBMED   17343995
  REMARK    GeneRIF: This investigation reveals that TPP II expression could be
            regulated through both positive and negative regulatory elements.
REFERENCE   4  (bases 1 to 5445)
  AUTHORS   York,I.A., Bhutani,N., Zendzian,S., Goldberg,A.L. and Rock,K.L.
  TITLE     Tripeptidyl peptidase II is the major peptidase needed to trim long
            antigenic precursors, but is not required for most MHC class I
            antigen presentation
  JOURNAL   J Immunol 177 (3), 1434-1443 (2006)
   PUBMED   16849449
  REMARK    GeneRIF: TPP2 plays a specialized role in antigen processing and
            one that is not essential for the generation of most presented
            peptides.
REFERENCE   5  (bases 1 to 5445)
  AUTHORS   Lindas,A.C. and Tomkinson,B.
  TITLE     Identification and characterization of the promoter for the gene
            encoding human tripeptidyl-peptidase II
  JOURNAL   Gene 345 (2), 249-257 (2005)
   PUBMED   15716107
  REMARK    GeneRIF: the promoter could be localized to a 215 bp fragment
            upstream of the initiation codon.
REFERENCE   6  (bases 1 to 5445)
  AUTHORS   Geier,E., Pfeifer,G., Wilm,M., Lucchiari-Hartz,M., Baumeister,W.,
            Eichmann,K. and Niedermann,G.
  TITLE     A giant protease with potential to substitute for some functions of
            the proteasome
  JOURNAL   Science 283 (5404), 978-981 (1999)
   PUBMED   9974389
REFERENCE   7  (bases 1 to 5445)
  AUTHORS   Tomkinson,B. and Jonsson,A.K.
  TITLE     Characterization of cDNA for human tripeptidyl peptidase II: the
            N-terminal part of the enzyme is similar to subtilisin
  JOURNAL   Biochemistry 30 (1), 168-174 (1991)
   PUBMED   1670990
REFERENCE   8  (bases 1 to 5445)
  AUTHORS   Tomkinson,B.
  TITLE     Nucleotide sequence of cDNA covering the N-terminus of human
            tripeptidyl peptidase II
  JOURNAL   Biomed Biochim Acta 50 (4-6), 727-729 (1991)
   PUBMED   1840501
REFERENCE   9  (bases 1 to 5445)
  AUTHORS   Tomkinson,B. and Zetterqvist,O.
  TITLE     Immunological cross-reactivity between human tripeptidyl peptidase
            II and fibronectin
  JOURNAL   Biochem J 267 (1), 149-154 (1990)
   PUBMED   1691635
REFERENCE   10 (bases 1 to 5445)
  AUTHORS   Tomkinson,B., Wernstedt,C., Hellman,U. and Zetterqvist,O.
  TITLE     Active site of tripeptidyl peptidase II from human erythrocytes is
            of the subtilisin type
  JOURNAL   Proc Natl Acad Sci U S A 84 (21), 7508-7512 (1987)
   PUBMED   3313395
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AU132468.1, BC039905.1,
            M73047.1, BF514671.1, N27441.1, DB303355.1 and BM684792.1.
            
            On Jan 1, 2019 this sequence version replaced NM_003291.3.
            
            Summary: This gene encodes a mammalian peptidase that, at neutral
            pH, removes tripeptides from the N terminus of longer peptides. The
            protein has a specialized function that is essential for some MHC
            class I antigen presentation. The protein is a high molecular mass
            serine exopeptidase; the amino acid sequence surrounding the serine
            residue at the active site is similar to the peptidases of the
            subtilisin class rather than the trypsin class. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the shortest
            transcript and encodes the shortest, but most predominant, isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.207306.1,
                                           SRR1803611.74573.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                AU132468.1         51-67
            18-37               BC039905.1         11-30
            38-806              M73047.1           8-776
            807-832             BC039905.1         800-825
            833-4302            M73047.1           803-4272
            4303-4661           BF514671.1         15-373              c
            4662-4912           N27441.1           5-255               c
            4913-5129           DB303355.1         316-532             c
            5130-5445           BM684792.1         16-331              c
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q33.1"
     gene            1..5445
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /note="tripeptidyl peptidase 2"
                     /db_xref="GeneID:7174"
                     /db_xref="HGNC:HGNC:12016"
                     /db_xref="MIM:190470"
     exon            1..218
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     CDS             54..3803
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /EC_number="3.4.14.10"
                     /note="isoform 1 is encoded by transcript variant 1;
                     tripeptidyl-peptidase II; tripeptidyl aminopeptidase"
                     /codon_start=1
                     /product="tripeptidyl-peptidase 2 isoform 1"
                     /protein_id="NP_003282.2"
                     /db_xref="CCDS:CCDS9502.1"
                     /db_xref="GeneID:7174"
                     /db_xref="HGNC:HGNC:12016"
                     /db_xref="MIM:190470"
                     /translation="MATAATEEPFPFHGLLPKKETGAASFLCRYPEYDGRGVLIAVLD
                     TGVDPGAPGMQVTTDGKPKIVDIIDTTGSGDVNTATEVEPKDGEIVGLSGRVLKIPAS
                     WTNPSGKYHIGIKNGYDFYPKALKERIQKERKEKIWDPVHRVALAEACRKQEEFDVAN
                     NGSSQANKLIKEELQSQVELLNSFEKKYSDPGPVYDCLVWHDGEVWRACIDSNEDGDL
                     SKSTVLRNYKEAQEYGSFGTAEMLNYSVNIYDDGNLLSIVTSGGAHGTHVASIAAGHF
                     PEEPERNGVAPGAQILSIKIGDTRLSTMETGTGLIRAMIEVINHKCDLVNYSYGEATH
                     WPNSGRICEVINEAVWKHNIIYVSSAGNNGPCLSTVGCPGGTTSSVIGVGAYVSPDMM
                     VAEYSLREKLPANQYTWSSRGPSADGALGVSISAPGGAIASVPNWTLRGTQLMNGTSM
                     SSPNACGGIALILSGLKANNIDYTVHSVRRALENTAVKADNIEVFAQGHGIIQVDKAY
                     DYLVQNTSFANKLGFTVTVGNNRGIYLRDPVQVAAPSDHGVGIEPVFPENTENSEKIS
                     LQLHLALTSNSSWVQCPSHLELMNQCRHINIRVDPRGLREGLHYTEVCGYDIASPNAG
                     PLFRVPITAVIAAKVNESSHYDLAFTDVHFKPGQIRRHFIEVPEGATWAEVTVCSCSS
                     EVSAKFVLHAVQLVKQRAYRSHEFYKFCSLPEKGTLTEAFPVLGGKAIEFCIARWWAS
                     LSDVNIDYTISFHGIVCTAPQLNIHASEGINRFDVQSSLKYEDLAPCITLKNWVQTLR
                     PVSAKTKPLGSRDVLPNNRQLYEMVLTYNFHQPKSGEVTPSCPLLCELLYESEFDSQL
                     WIIFDQNKRQMGSGDAYPHQYSLKLEKGDYTIRLQIRHEQISDLERLKDLPFIVSHRL
                     SNTLSLDIHENHSFALLGKKKSSNLTLPPKYNQPFFVTSLPDDKIPKGAGPGCYLAGS
                     LTLSKTELGKKADVIPVHYYLIPPPTKTKNGSKDKEKDSEKEKDLKEEFTEALRDLKI
                     QWMTKLDSSDIYNELKETYPNYLPLYVARLHQLDAEKERMKRLNEIVDAANAVISHID
                     QTALAVYIAMKTDPRPDAATIKNDMDKQKSTLVDALCRKGCALADHLLHTQAQDGAIS
                     TDAEGKEEEGESPLDSLAETFWETTKWTDLFDNKVLTFAYKHALVNKMYGRGLKFATK
                     LVEEKPTKENWKNCIQLMKLLGWTHCASFTENWLPIMYPPDYCVF"
     exon            219..347
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            348..443
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            444..548
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            549..673
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            674..837
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            838..992
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            993..1069
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1197
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1297
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1446
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1447..1562
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1563..1731
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1732..1889
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1890..1966
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1967..2073
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2228
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            2229..2345
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            2346..2446
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            2447..2543
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            2544..2681
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            2682..2926
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            2927..3005
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            3006..3157
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            3158..3254
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            3255..3385
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            3386..3593
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            3594..3713
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            3714..5445
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3921..3926
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /note="hexamer: ATTAAA"
     polyA_site      3948
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /note="major polyA site"
     regulatory      4633..4638
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /note="hexamer: AATAAA"
     polyA_site      4661
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /note="major polyA site"
     regulatory      4894..4899
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /note="hexamer: AATAAA"
     polyA_site      4921
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
     polyA_site      4948
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
     regulatory      5424..5429
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /note="hexamer: AATAAA"
     polyA_site      5445
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
ORIGIN      
        1 agtccgcgcg cagcctggca gtttgccgct tcctcgtcct ccatcctgcg tccatggcca
       61 ccgctgcgac tgaggagccc ttcccttttc acggtctcct gccgaagaag gagaccggag
      121 ccgcctcctt cctctgccgc tacccggagt atgatgggcg gggggtgctc atcgcagtcc
      181 tggacacggg ggtcgacccg ggggctccgg gcatgcaggt tacaactgat ggaaaaccaa
      241 aaatcgttga tatcattgat acaacaggaa gtggcgatgt gaatactgct acagaagtag
      301 agccaaagga tggtgagatt gttggccttt caggaagagt gcttaagatt cctgcaagct
      361 ggacaaatcc ctcaggcaaa tatcatattg gcataaaaaa tggctatgac ttctatccta
      421 aggcactcaa ggaaaggata cagaaagaac ggaaggaaaa aatctgggac cctgttcaca
      481 gagtggccct tgcagaagcc tgtagaaaac aggaagaatt tgatgttgcc aacaacggct
      541 cttctcaagc aaataaacta atcaaggagg aacttcaaag tcaagtggaa ttgctaaatt
      601 cttttgagaa gaaatacagc gatcctggcc ctgtatatga ctgcttggta tggcatgatg
      661 gcgaagtctg gagagcctgc attgattcta atgaagatgg ggacttgagt aaatctaccg
      721 tgttgagaaa ctacaaagaa gcccaagaat atggctcttt tggcacagct gagatgttga
      781 attactccgt taatatatac gatgatggaa acctgctctc cattgtgacc agtggaggag
      841 ctcatgggac acatgtagct agtatagctg ctggacactt tccagaagaa cctgaacgga
      901 atggggtagc tcctggtgct caaattcttt ccatcaagat tggtgataca agactaagca
      961 caatggaaac aggcacaggc ctcataagag ctatgataga agttataaat cataagtgtg
     1021 atcttgtcaa ctacagttac ggagaagcaa ctcactggcc aaattctggg agaatttgtg
     1081 aagtaattaa tgaagcagta tggaagcata atataattta tgtttcaagt gctggaaata
     1141 atggtccatg cctgtctaca gttggttgtc caggtggaac tacatcaagt gtgataggtg
     1201 ttggtgctta tgtttctcct gatatgatgg ttgctgagta ttcactgaga gagaaattac
     1261 ctgcaaatca atatacttgg tcttctagag gacctagtgc tgacggggcc cttggtgtga
     1321 gtatcagtgc gccaggagga gccattgctt ctgttcctaa ctggacactg agagggacgc
     1381 agctgatgaa tggaacatct atgtcttccc ccaatgcatg tggaggcatt gccctgatcc
     1441 tttcaggtct gaaagctaat aacattgact acacagttca ttcagtcaga agagctctag
     1501 aaaacactgc agtgaaggct gacaatatag aagtatttgc tcaaggacat ggtattattc
     1561 aggttgataa agcctatgac tacctcgttc agaatacatc atttgctaat aaattaggtt
     1621 ttactgttac tgttggaaat aaccgtggca tctacctccg agatcctgtt caggtggctg
     1681 caccttcaga tcatggcgtt ggcattgaac ctgtatttcc ggagaacaca gaaaactctg
     1741 aaaaaatatc ccttcagctt catttagctc tgacttcaaa ttcatcttgg gttcagtgtc
     1801 ccagccattt ggaactcatg aatcaatgta gacacataaa catacgtgtg gatcccaggg
     1861 gcttaagaga aggattgcat tatacagagg tatgtggcta tgatatagca tcccctaacg
     1921 caggtccgct cttcagagtt ccgatcactg cagttatagc agcaaaagta aatgaatcat
     1981 cacattatga tctagccttt acagatgtac actttaaacc tggtcaaatt cgaaggcatt
     2041 ttattgaggt tcctgagggt gcaacatggg ctgaagtgac agtgtgttcg tgttcttctg
     2101 aggtgtcagc aaagtttgtt ctacatgcag tccagcttgt gaagcaaaga gcatatcgaa
     2161 gccatgaatt ctataagttt tgttctcttc cagagaaagg aacactgact gaagcttttc
     2221 ctgtcctagg tggaaaagca attgaatttt gcattgctcg ttggtgggca agtctcagtg
     2281 atgtcaacat tgattatacc atttctttcc atgggatagt gtgtactgct cctcagttaa
     2341 acattcatgc atcggaagga atcaaccgct ttgatgttca gtcctccttg aaatacgaag
     2401 atctggctcc ctgcataact ttgaagaact gggtccaaac actgcgccca gtgagtgcaa
     2461 aaacaaaacc tttaggatca agagatgttt tgccaaataa ccgtcaactt tatgagatgg
     2521 tcctgacata taactttcat caacccaaga gtggggaagt aactccaagc tgcccactac
     2581 tttgtgaact attatatgaa tctgaatttg acagccaact gtggattatt tttgaccaga
     2641 acaaaagaca gatgggttca ggcgatgcct atccacatca gtattctttg aaactggaga
     2701 aaggagatta tacaattcga ctacagattc gccatgagca aatcagtgat ttggaacgcc
     2761 ttaaagacct tccatttatt gtttctcata gattgtctaa taccttgagc ttagatattc
     2821 atgaaaatca tagttttgca cttctaggga agaagaaatc aagcaatttg acattaccac
     2881 ccaaatataa ccagccattc tttgttactt ccttacctga tgataaaata cctaaagggg
     2941 caggacctgg atgctatctt gcaggatcct taacattgtc aaagactgaa ctaggaaaga
     3001 aagctgatgt aatccctgtt cattactact taatacctcc accaacaaag actaagaatg
     3061 gcagcaaaga taaggaaaaa gattcagaaa aagagaaaga tttaaaagaa gagtttactg
     3121 aagcattacg agatcttaaa attcagtgga tgacaaagct ggattctagt gacatttata
     3181 acgaattgaa agaaacatat cctaattatc ttcctctgta cgttgcacga cttcatcaat
     3241 tggatgctga aaaggaacga atgaaaagac ttaatgaaat tgttgatgcg gcaaatgctg
     3301 ttatttctca tatagatcaa acagccctag cagtttatat tgcaatgaag actgatccca
     3361 ggcctgatgc agctactata aaaaatgaca tggacaaaca aaaatccacc ctcgtagatg
     3421 ccctttgtag gaaaggttgt gccctggcag accatcttct tcacacccag gctcaagacg
     3481 gagccatttc cactgatgca gaaggaaagg aggaggaagg agaaagtcct ttggattctc
     3541 tggcagaaac attttgggaa actactaaat ggactgatct ctttgacaat aaggttttga
     3601 catttgcata taaacatgca ttagtaaata aaatgtatgg gagaggcctt aaatttgcaa
     3661 ctaaacttgt ggaagaaaaa ccaacaaaag aaaactggaa aaattgtatt caactgatga
     3721 agttacttgg atggacccat tgtgcatctt ttactgaaaa ctggctcccc atcatgtatc
     3781 ctcccgatta ttgcgtattc taaaatagga aacaagactt taaattttaa aaaaggaagt
     3841 tttatagtga atgggtataa aaacaaattt gtggcatttt tagtctaatg catgttttca
     3901 tccactatcc agtactgatt attaaaatga catgtattta tcagagaatt cactgacgtg
     3961 tggcttaata catgtaaatc tagacctctg acatcatggt gttttcttaa tgcctcacat
     4021 tgctggcacg gggatgtgcc ctgcctgcca gcacctagga cttcgagttg ggttgcagct
     4081 tatgacatgc atgataggtt ttggaaggta acttttaact gcaaacctat aaagtactat
     4141 tttttatttt ataaatgaac agggttttaa cgtgctcaac tttaattttt ttcaattgta
     4201 tgaaggcctt aaaaaagcta cattaagcgt agctaaaatt atttattgga ctaaaaacta
     4261 acagaacttc atttccagaa tttttttttt tttttttttt ttttggcaaa tgtttacatt
     4321 caattaaggg gaaaaagtag aaccagcaca aatgagtggc agttgctgga gcataactgc
     4381 ttcaataaat cttcatcttg gggtaattac aggcaagtca ttttcacatc ctcttgaggt
     4441 tcagagcatc agaatgaact ctatgaatac atgtgtaagt gccagacagc tgaatcttta
     4501 tcaggtattg taaagataca catatgatat gtttattaaa attgaaataa tgtaaaacac
     4561 atgaataaat ttgcaaaacc aagatcacag tacaccatat gcactctggt accttaattt
     4621 ttttttataa ataataaaag tgaatattga agcttcttaa agttggtctt aatttttcat
     4681 aaataaaatt tggggttata acagaaataa tacattaaat acatagacaa aaaaagttaa
     4741 acataagata atctatttta cagtgtttct tataggctta caattatttg aatgtatttc
     4801 cagttcttgt atgattaact tttgtgcata tctaactttt tattattttt atctttggct
     4861 actgcacgtg catctctcgt cattcctcca ccaaataaac agacaaaagt agtattgaaa
     4921 agtttacagt cccttatcta atggcaaatt gtgtgtatgt atgatttttt tatcttgttc
     4981 tcttaagatt tactgctgct taaaattact accagtaatc cttagtcact cccaactgtt
     5041 tctgctgtat ctcatgaagt gttagagcgt ttatgagaaa cataaataca tgtataagca
     5101 tattggtcac accttctggg ctagctaggg tccaaagtct atccatgaaa gctacatgca
     5161 tgttctctct cacacactca tggactttca cctgcacaca catacaggtc cccatttgta
     5221 taggtttagt ccagtaagag aatggagaag ctcagtaaag gtaggatgac ttcccaaggt
     5281 tatacggctg atcaactgtt ggaaattaat tacagacctt gctttacaac ccaggttttg
     5341 ctatttcagg taagtactac ctacagcagg aatcagtaac gtttggccct ttaacccaaa
     5401 ctggactgct gcctgttttt gtaaataaag ttttattgga acaga
//
