LOCUS       NM_001330588            5484 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens tripeptidyl peptidase 2 (TPP2), transcript variant 2,
            mRNA.
ACCESSION   NM_001330588 XM_005254072
VERSION     NM_001330588.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Liao,C.L., Hu,R.C., Liao,M.S., Chen,Y.J., Chen,Y.P., Hsieh,H.H.,
            Tai,C.H., Chou,T.C., Chu,C.Y., Chen,Y.J., Lo,L.C. and Lin,J.J.
  TITLE     Unveiling a novel serpinB2-tripeptidyl peptidase II signaling axis
            during senescence
  JOURNAL   J Cell Sci 135 (10) (2022)
   PUBMED   35466366
  REMARK    GeneRIF: Unveiling a novel serpinB2-tripeptidyl peptidase II
            signaling axis during senescence.
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Atallah,I., Quinodoz,M., Campos-Xavier,B., Peter,V.G., Fouriki,A.,
            Bonvin,C., Bottani,A., Kumps,C., Angelini,F., Bellutti Enders,F.,
            Christen-Zaech,S., Rizzi,M., Renella,R., Beck-Popovic,M.,
            Poloni,C., Frossard,V., Blouin,J.L., Rivolta,C., Riccio,O.,
            Candotti,F., Hofer,M., Unger,S. and Superti-Furga,A.
  TITLE     Immune deficiency, autoimmune disease and intellectual disability:
            A pleiotropic disorder caused by biallelic variants in the TPP2
            gene
  JOURNAL   Clin Genet 99 (6), 780-788 (2021)
   PUBMED   33586135
  REMARK    GeneRIF: Immune deficiency, autoimmune disease and intellectual
            disability: A pleiotropic disorder caused by biallelic variants in
            the TPP2 gene.
REFERENCE   3  (bases 1 to 5484)
  AUTHORS   Lindas,A.C. and Tomkinson,B.
  TITLE     Characterization of the promoter of the gene encoding human
            tripeptidyl-peptidase II and identification of upstream silencer
            elements
  JOURNAL   Gene 393 (1-2), 62-69 (2007)
   PUBMED   17343995
  REMARK    GeneRIF: This investigation reveals that TPP II expression could be
            regulated through both positive and negative regulatory elements.
REFERENCE   4  (bases 1 to 5484)
  AUTHORS   York,I.A., Bhutani,N., Zendzian,S., Goldberg,A.L. and Rock,K.L.
  TITLE     Tripeptidyl peptidase II is the major peptidase needed to trim long
            antigenic precursors, but is not required for most MHC class I
            antigen presentation
  JOURNAL   J Immunol 177 (3), 1434-1443 (2006)
   PUBMED   16849449
  REMARK    GeneRIF: TPP2 plays a specialized role in antigen processing and
            one that is not essential for the generation of most presented
            peptides.
REFERENCE   5  (bases 1 to 5484)
  AUTHORS   Lindas,A.C. and Tomkinson,B.
  TITLE     Identification and characterization of the promoter for the gene
            encoding human tripeptidyl-peptidase II
  JOURNAL   Gene 345 (2), 249-257 (2005)
   PUBMED   15716107
  REMARK    GeneRIF: the promoter could be localized to a 215 bp fragment
            upstream of the initiation codon.
REFERENCE   6  (bases 1 to 5484)
  AUTHORS   Geier,E., Pfeifer,G., Wilm,M., Lucchiari-Hartz,M., Baumeister,W.,
            Eichmann,K. and Niedermann,G.
  TITLE     A giant protease with potential to substitute for some functions of
            the proteasome
  JOURNAL   Science 283 (5404), 978-981 (1999)
   PUBMED   9974389
REFERENCE   7  (bases 1 to 5484)
  AUTHORS   Tomkinson,B. and Jonsson,A.K.
  TITLE     Characterization of cDNA for human tripeptidyl peptidase II: the
            N-terminal part of the enzyme is similar to subtilisin
  JOURNAL   Biochemistry 30 (1), 168-174 (1991)
   PUBMED   1670990
REFERENCE   8  (bases 1 to 5484)
  AUTHORS   Tomkinson,B.
  TITLE     Nucleotide sequence of cDNA covering the N-terminus of human
            tripeptidyl peptidase II
  JOURNAL   Biomed Biochim Acta 50 (4-6), 727-729 (1991)
   PUBMED   1840501
REFERENCE   9  (bases 1 to 5484)
  AUTHORS   Tomkinson,B. and Zetterqvist,O.
  TITLE     Immunological cross-reactivity between human tripeptidyl peptidase
            II and fibronectin
  JOURNAL   Biochem J 267 (1), 149-154 (1990)
   PUBMED   1691635
REFERENCE   10 (bases 1 to 5484)
  AUTHORS   Tomkinson,B., Wernstedt,C., Hellman,U. and Zetterqvist,O.
  TITLE     Active site of tripeptidyl peptidase II from human erythrocytes is
            of the subtilisin type
  JOURNAL   Proc Natl Acad Sci U S A 84 (21), 7508-7512 (1987)
   PUBMED   3313395
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AU132468.1, BC039905.1,
            M73047.1, BX370108.2, BF514671.1, N27441.1, DB303355.1 and
            BM684792.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Dec 31, 2018 this sequence version replaced NM_001330588.1.
            
            Summary: This gene encodes a mammalian peptidase that, at neutral
            pH, removes tripeptides from the N terminus of longer peptides. The
            protein has a specialized function that is essential for some MHC
            class I antigen presentation. The protein is a high molecular mass
            serine exopeptidase; the amino acid sequence surrounding the serine
            residue at the active site is similar to the peptidases of the
            subtilisin class rather than the trypsin class. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) contains an alternate in-frame
            exon in the 3' coding region, compared to variant 1. The encoded
            isoform (2) has the same N- and C-termini, but is longer than
            isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.71142.1,
                                           SRR1803617.222348.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000376052.5/ ENSP00000365220.3
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                AU132468.1         51-67
            18-37               BC039905.1         11-30
            38-806              M73047.1           8-776
            807-832             BC039905.1         800-825
            833-2363            M73047.1           803-2333
            2364-3118           BX370108.2         15-769
            3119-4341           M73047.1           3050-4272
            4342-4700           BF514671.1         15-373              c
            4701-4951           N27441.1           5-255               c
            4952-5168           DB303355.1         316-532             c
            5169-5484           BM684792.1         16-331              c
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q33.1"
     gene            1..5484
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /note="tripeptidyl peptidase 2"
                     /db_xref="GeneID:7174"
                     /db_xref="HGNC:HGNC:12016"
                     /db_xref="MIM:190470"
     exon            1..218
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     CDS             54..3842
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /EC_number="3.4.14.10"
                     /note="isoform 2 is encoded by transcript variant 2;
                     tripeptidyl-peptidase II; tripeptidyl aminopeptidase"
                     /codon_start=1
                     /product="tripeptidyl-peptidase 2 isoform 2"
                     /protein_id="NP_001317517.1"
                     /db_xref="CCDS:CCDS81777.1"
                     /db_xref="GeneID:7174"
                     /db_xref="HGNC:HGNC:12016"
                     /db_xref="MIM:190470"
                     /translation="MATAATEEPFPFHGLLPKKETGAASFLCRYPEYDGRGVLIAVLD
                     TGVDPGAPGMQVTTDGKPKIVDIIDTTGSGDVNTATEVEPKDGEIVGLSGRVLKIPAS
                     WTNPSGKYHIGIKNGYDFYPKALKERIQKERKEKIWDPVHRVALAEACRKQEEFDVAN
                     NGSSQANKLIKEELQSQVELLNSFEKKYSDPGPVYDCLVWHDGEVWRACIDSNEDGDL
                     SKSTVLRNYKEAQEYGSFGTAEMLNYSVNIYDDGNLLSIVTSGGAHGTHVASIAAGHF
                     PEEPERNGVAPGAQILSIKIGDTRLSTMETGTGLIRAMIEVINHKCDLVNYSYGEATH
                     WPNSGRICEVINEAVWKHNIIYVSSAGNNGPCLSTVGCPGGTTSSVIGVGAYVSPDMM
                     VAEYSLREKLPANQYTWSSRGPSADGALGVSISAPGGAIASVPNWTLRGTQLMNGTSM
                     SSPNACGGIALILSGLKANNIDYTVHSVRRALENTAVKADNIEVFAQGHGIIQVDKAY
                     DYLVQNTSFANKLGFTVTVGNNRGIYLRDPVQVAAPSDHGVGIEPVFPENTENSEKIS
                     LQLHLALTSNSSWVQCPSHLELMNQCRHINIRVDPRGLREGLHYTEVCGYDIASPNAG
                     PLFRVPITAVIAAKVNESSHYDLAFTDVHFKPGQIRRHFIEVPEGATWAEVTVCSCSS
                     EVSAKFVLHAVQLVKQRAYRSHEFYKFCSLPEKGTLTEAFPVLGGKAIEFCIARWWAS
                     LSDVNIDYTISFHGIVCTAPQLNIHASEGINRFDVQSSLKYEDLAPCITLKNWVQTLR
                     PVSAKTKPLGSRDVLPNNRQLYEMVLTYNFHQPKSGEVTPSCPLLCELLYESEFDSQL
                     WIIFDQNKRQMGSGDAYPHQYSLKLEKGDYTIRLQIRHEQISDLERLKDLPFIVSHRL
                     SNTLSLDIHENHSFALLGKKKSSNLTLPPKYNQPFFVTSLPDDKIPKGAGPGCYLAGS
                     LTLSKTELGKKAGQSAAKRQGKFKKDVIPVHYYLIPPPTKTKNGSKDKEKDSEKEKDL
                     KEEFTEALRDLKIQWMTKLDSSDIYNELKETYPNYLPLYVARLHQLDAEKERMKRLNE
                     IVDAANAVISHIDQTALAVYIAMKTDPRPDAATIKNDMDKQKSTLVDALCRKGCALAD
                     HLLHTQAQDGAISTDAEGKEEEGESPLDSLAETFWETTKWTDLFDNKVLTFAYKHALV
                     NKMYGRGLKFATKLVEEKPTKENWKNCIQLMKLLGWTHCASFTENWLPIMYPPDYCVF
                     "
     exon            219..347
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            348..443
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            444..548
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            549..673
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            674..837
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            838..992
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            993..1069
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1197
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1297
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1446
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1447..1562
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1563..1731
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1732..1889
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1890..1966
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1967..2073
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2228
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            2229..2345
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            2346..2446
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            2447..2543
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            2544..2681
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            2682..2926
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            2927..3005
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            3006..3044
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            3045..3196
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            3197..3293
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            3294..3424
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            3425..3632
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            3633..3752
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     exon            3753..5484
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3960..3965
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /note="hexamer: ATTAAA"
     polyA_site      3987
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /note="major polyA site"
     regulatory      4672..4677
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /note="hexamer: AATAAA"
     polyA_site      4700
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /note="major polyA site"
     regulatory      4933..4938
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /note="hexamer: AATAAA"
     polyA_site      4960
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
     polyA_site      4987
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
     regulatory      5463..5468
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
                     /note="hexamer: AATAAA"
     polyA_site      5484
                     /gene="TPP2"
                     /gene_synonym="IMD78; TPP-2; TPP-II; TPPII"
ORIGIN      
        1 agtccgcgcg cagcctggca gtttgccgct tcctcgtcct ccatcctgcg tccatggcca
       61 ccgctgcgac tgaggagccc ttcccttttc acggtctcct gccgaagaag gagaccggag
      121 ccgcctcctt cctctgccgc tacccggagt atgatgggcg gggggtgctc atcgcagtcc
      181 tggacacggg ggtcgacccg ggggctccgg gcatgcaggt tacaactgat ggaaaaccaa
      241 aaatcgttga tatcattgat acaacaggaa gtggcgatgt gaatactgct acagaagtag
      301 agccaaagga tggtgagatt gttggccttt caggaagagt gcttaagatt cctgcaagct
      361 ggacaaatcc ctcaggcaaa tatcatattg gcataaaaaa tggctatgac ttctatccta
      421 aggcactcaa ggaaaggata cagaaagaac ggaaggaaaa aatctgggac cctgttcaca
      481 gagtggccct tgcagaagcc tgtagaaaac aggaagaatt tgatgttgcc aacaacggct
      541 cttctcaagc aaataaacta atcaaggagg aacttcaaag tcaagtggaa ttgctaaatt
      601 cttttgagaa gaaatacagc gatcctggcc ctgtatatga ctgcttggta tggcatgatg
      661 gcgaagtctg gagagcctgc attgattcta atgaagatgg ggacttgagt aaatctaccg
      721 tgttgagaaa ctacaaagaa gcccaagaat atggctcttt tggcacagct gagatgttga
      781 attactccgt taatatatac gatgatggaa acctgctctc cattgtgacc agtggaggag
      841 ctcatgggac acatgtagct agtatagctg ctggacactt tccagaagaa cctgaacgga
      901 atggggtagc tcctggtgct caaattcttt ccatcaagat tggtgataca agactaagca
      961 caatggaaac aggcacaggc ctcataagag ctatgataga agttataaat cataagtgtg
     1021 atcttgtcaa ctacagttac ggagaagcaa ctcactggcc aaattctggg agaatttgtg
     1081 aagtaattaa tgaagcagta tggaagcata atataattta tgtttcaagt gctggaaata
     1141 atggtccatg cctgtctaca gttggttgtc caggtggaac tacatcaagt gtgataggtg
     1201 ttggtgctta tgtttctcct gatatgatgg ttgctgagta ttcactgaga gagaaattac
     1261 ctgcaaatca atatacttgg tcttctagag gacctagtgc tgacggggcc cttggtgtga
     1321 gtatcagtgc gccaggagga gccattgctt ctgttcctaa ctggacactg agagggacgc
     1381 agctgatgaa tggaacatct atgtcttccc ccaatgcatg tggaggcatt gccctgatcc
     1441 tttcaggtct gaaagctaat aacattgact acacagttca ttcagtcaga agagctctag
     1501 aaaacactgc agtgaaggct gacaatatag aagtatttgc tcaaggacat ggtattattc
     1561 aggttgataa agcctatgac tacctcgttc agaatacatc atttgctaat aaattaggtt
     1621 ttactgttac tgttggaaat aaccgtggca tctacctccg agatcctgtt caggtggctg
     1681 caccttcaga tcatggcgtt ggcattgaac ctgtatttcc ggagaacaca gaaaactctg
     1741 aaaaaatatc ccttcagctt catttagctc tgacttcaaa ttcatcttgg gttcagtgtc
     1801 ccagccattt ggaactcatg aatcaatgta gacacataaa catacgtgtg gatcccaggg
     1861 gcttaagaga aggattgcat tatacagagg tatgtggcta tgatatagca tcccctaacg
     1921 caggtccgct cttcagagtt ccgatcactg cagttatagc agcaaaagta aatgaatcat
     1981 cacattatga tctagccttt acagatgtac actttaaacc tggtcaaatt cgaaggcatt
     2041 ttattgaggt tcctgagggt gcaacatggg ctgaagtgac agtgtgttcg tgttcttctg
     2101 aggtgtcagc aaagtttgtt ctacatgcag tccagcttgt gaagcaaaga gcatatcgaa
     2161 gccatgaatt ctataagttt tgttctcttc cagagaaagg aacactgact gaagcttttc
     2221 ctgtcctagg tggaaaagca attgaatttt gcattgctcg ttggtgggca agtctcagtg
     2281 atgtcaacat tgattatacc atttctttcc atgggatagt gtgtactgct cctcagttaa
     2341 acattcatgc atcggaagga atcaaccgct ttgatgttca gtcctccttg aaatacgaag
     2401 atctggctcc ctgcataact ttgaagaact gggtccaaac actgcgccca gtgagtgcaa
     2461 aaacaaaacc tttaggatca agagatgttt tgccaaataa ccgtcaactt tatgagatgg
     2521 tcctgacata taactttcat caacccaaga gtggggaagt aactccaagc tgcccactac
     2581 tttgtgaact attatatgaa tctgaatttg acagccaact gtggattatt tttgaccaga
     2641 acaaaagaca gatgggttca ggcgatgcct atccacatca gtattctttg aaactggaga
     2701 aaggagatta tacaattcga ctacagattc gccatgagca aatcagtgat ttggaacgcc
     2761 ttaaagacct tccatttatt gtttctcata gattgtctaa taccttgagc ttagatattc
     2821 atgaaaatca tagttttgca cttctaggga agaagaaatc aagcaatttg acattaccac
     2881 ccaaatataa ccagccattc tttgttactt ccttacctga tgataaaata cctaaagggg
     2941 caggacctgg atgctatctt gcaggatcct taacattgtc aaagactgaa ctaggaaaga
     3001 aagctgggca gtctgcagca aaacgacaag gaaaatttaa aaaggatgta atccctgttc
     3061 attactactt aatacctcca ccaacaaaga ctaagaatgg cagcaaagat aaggaaaaag
     3121 attcagaaaa agagaaagat ttaaaagaag agtttactga agcattacga gatcttaaaa
     3181 ttcagtggat gacaaagctg gattctagtg acatttataa cgaattgaaa gaaacatatc
     3241 ctaattatct tcctctgtac gttgcacgac ttcatcaatt ggatgctgaa aaggaacgaa
     3301 tgaaaagact taatgaaatt gttgatgcgg caaatgctgt tatttctcat atagatcaaa
     3361 cagccctagc agtttatatt gcaatgaaga ctgatcccag gcctgatgca gctactataa
     3421 aaaatgacat ggacaaacaa aaatccaccc tcgtagatgc cctttgtagg aaaggttgtg
     3481 ccctggcaga ccatcttctt cacacccagg ctcaagacgg agccatttcc actgatgcag
     3541 aaggaaagga ggaggaagga gaaagtcctt tggattctct ggcagaaaca ttttgggaaa
     3601 ctactaaatg gactgatctc tttgacaata aggttttgac atttgcatat aaacatgcat
     3661 tagtaaataa aatgtatggg agaggcctta aatttgcaac taaacttgtg gaagaaaaac
     3721 caacaaaaga aaactggaaa aattgtattc aactgatgaa gttacttgga tggacccatt
     3781 gtgcatcttt tactgaaaac tggctcccca tcatgtatcc tcccgattat tgcgtattct
     3841 aaaataggaa acaagacttt aaattttaaa aaaggaagtt ttatagtgaa tgggtataaa
     3901 aacaaatttg tggcattttt agtctaatgc atgttttcat ccactatcca gtactgatta
     3961 ttaaaatgac atgtatttat cagagaattc actgacgtgt ggcttaatac atgtaaatct
     4021 agacctctga catcatggtg ttttcttaat gcctcacatt gctggcacgg ggatgtgccc
     4081 tgcctgccag cacctaggac ttcgagttgg gttgcagctt atgacatgca tgataggttt
     4141 tggaaggtaa cttttaactg caaacctata aagtactatt ttttatttta taaatgaaca
     4201 gggttttaac gtgctcaact ttaatttttt tcaattgtat gaaggcctta aaaaagctac
     4261 attaagcgta gctaaaatta tttattggac taaaaactaa cagaacttca tttccagaat
     4321 tttttttttt tttttttttt tttggcaaat gtttacattc aattaagggg aaaaagtaga
     4381 accagcacaa atgagtggca gttgctggag cataactgct tcaataaatc ttcatcttgg
     4441 ggtaattaca ggcaagtcat tttcacatcc tcttgaggtt cagagcatca gaatgaactc
     4501 tatgaataca tgtgtaagtg ccagacagct gaatctttat caggtattgt aaagatacac
     4561 atatgatatg tttattaaaa ttgaaataat gtaaaacaca tgaataaatt tgcaaaacca
     4621 agatcacagt acaccatatg cactctggta ccttaatttt tttttataaa taataaaagt
     4681 gaatattgaa gcttcttaaa gttggtctta atttttcata aataaaattt ggggttataa
     4741 cagaaataat acattaaata catagacaaa aaaagttaaa cataagataa tctattttac
     4801 agtgtttctt ataggcttac aattatttga atgtatttcc agttcttgta tgattaactt
     4861 ttgtgcatat ctaacttttt attattttta tctttggcta ctgcacgtgc atctctcgtc
     4921 attcctccac caaataaaca gacaaaagta gtattgaaaa gtttacagtc ccttatctaa
     4981 tggcaaattg tgtgtatgta tgattttttt atcttgttct cttaagattt actgctgctt
     5041 aaaattacta ccagtaatcc ttagtcactc ccaactgttt ctgctgtatc tcatgaagtg
     5101 ttagagcgtt tatgagaaac ataaatacat gtataagcat attggtcaca ccttctgggc
     5161 tagctagggt ccaaagtcta tccatgaaag ctacatgcat gttctctctc acacactcat
     5221 ggactttcac ctgcacacac atacaggtcc ccatttgtat aggtttagtc cagtaagaga
     5281 atggagaagc tcagtaaagg taggatgact tcccaaggtt atacggctga tcaactgttg
     5341 gaaattaatt acagaccttg ctttacaacc caggttttgc tatttcaggt aagtactacc
     5401 tacagcagga atcagtaacg tttggccctt taacccaaac tggactgctg cctgtttttg
     5461 taaataaagt tttattggaa caga
//
