LOCUS       NM_003290               2474 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens tropomyosin 4 (TPM4), transcript variant 1, mRNA.
ACCESSION   NM_003290
VERSION     NM_003290.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Yan,J., Xie,B., Tian,Y., An,W., Peng,Z., Liu,Z., Li,J. and Li,L.
  TITLE     MicroRNA-5195-3p mediated malignant biological behaviour of
            insulin-resistant liver cancer cells via SOX9 and TPM4
  JOURNAL   BMC Cancer 23 (1), 557 (2023)
   PUBMED   37328795
  REMARK    GeneRIF: MicroRNA-5195-3p mediated malignant biological behaviour
            of insulin-resistant liver cancer cells via SOX9 and TPM4.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Guo,Q., Zhao,L., Yan,N., Li,Y., Guo,C., Dang,S., Shen,X., Han,J.
            and Luo,Y.
  TITLE     Integrated pan-cancer analysis and experimental verification of the
            roles of tropomyosin 4 in gastric cancer
  JOURNAL   Front Immunol 14, 1148056 (2023)
   PUBMED   36993958
  REMARK    GeneRIF: Integrated pan-cancer analysis and experimental
            verification of the roles of tropomyosin 4 in gastric cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2474)
  AUTHORS   Luo,A., Lan,X., Qiu,Q., Zhou,Q., Li,J., Wu,M., Liu,P., Zhang,H.,
            Lu,B., Lu,Y. and Lu,W.
  TITLE     LncRNA SFTA1P promotes cervical cancer progression by interaction
            with PTBP1 to facilitate TPM4 mRNA degradation
  JOURNAL   Cell Death Dis 13 (11), 936 (2022)
   PUBMED   36344495
  REMARK    GeneRIF: LncRNA SFTA1P promotes cervical cancer progression by
            interaction with PTBP1 to facilitate TPM4 mRNA degradation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2474)
  AUTHORS   Marin-Quilez,A., Vuelta,E., Diaz-Ajenjo,L., Fernandez-Infante,C.,
            Garcia-Tunon,I., Benito,R., Palma-Barqueros,V.,
            Hernandez-Rivas,J.M., Gonzalez-Porras,J.R., Rivera,J. and
            Bastida,J.M.
  TITLE     A novel nonsense variant in TPM4 caused dominant
            macrothrombocytopenia, mild bleeding tendency and disrupted
            cytoskeleton remodeling
  JOURNAL   J Thromb Haemost 20 (5), 1248-1255 (2022)
   PUBMED   35170221
REFERENCE   5  (bases 1 to 2474)
  AUTHORS   Stapley,R.J., Poulter,N.S., Khan,A.O., Smith,C.W., Bignell,P.,
            Fratter,C., Lester,W., Lowe,G. and Morgan,N.V.
  CONSRTM   UK GAPP Study Group
  TITLE     Rare missense variants in Tropomyosin-4 (TPM4) are associated with
            platelet dysfunction, cytoskeletal defects, and excessive bleeding
  JOURNAL   J Thromb Haemost 20 (2), 478-485 (2022)
   PUBMED   34758189
  REMARK    GeneRIF: Rare missense variants in Tropomyosin-4 (TPM4) are
            associated with platelet dysfunction, cytoskeletal defects, and
            excessive bleeding.
            Erratum:[J Thromb Haemost. 2022 Jun;20(6):1511. doi:
            10.1111/jth.15719.. PMID: 35596517]
REFERENCE   6  (bases 1 to 2474)
  AUTHORS   Geeves,M.A., Hitchcock-DeGregori,S.E. and Gunning,P.W.
  TITLE     A systematic nomenclature for mammalian tropomyosin isoforms
  JOURNAL   J Muscle Res Cell Motil 36 (2), 147-153 (2015)
   PUBMED   25369766
  REMARK    Review article
REFERENCE   7  (bases 1 to 2474)
  AUTHORS   Rasmussen,H.H., van Damme,J., Puype,M., Gesser,B., Celis,J.E. and
            Vandekerckhove,J.
  TITLE     Microsequences of 145 proteins recorded in the two-dimensional gel
            protein database of normal human epidermal keratinocytes
  JOURNAL   Electrophoresis 13 (12), 960-969 (1992)
   PUBMED   1286667
REFERENCE   8  (bases 1 to 2474)
  AUTHORS   Crabos,M., Yamakado,T., Heizmann,C.W., Cerletti,N., Buhler,F.R. and
            Erne,P.
  TITLE     The calcium binding protein tropomyosin in human platelets and
            cardiac tissue: elevation in hypertensive cardiac hypertrophy
  JOURNAL   Eur J Clin Invest 21 (5), 472-478 (1991)
   PUBMED   1836432
REFERENCE   9  (bases 1 to 2474)
  AUTHORS   MacLeod,A.R., Talbot,K., Smillie,L.B. and Houlker,C.
  TITLE     Characterization of a cDNA defining a gene family encoding TM30p1,
            a human fibroblast tropomyosin
  JOURNAL   J Mol Biol 194 (1), 1-10 (1987)
   PUBMED   3612796
REFERENCE   10 (bases 1 to 2474)
  AUTHORS   MacLeod,A.R., Houlker,C., Reinach,F.C., Smillie,L.B., Talbot,K.,
            Modi,G. and Walsh,F.S.
  TITLE     A muscle-type tropomyosin in human fibroblasts: evidence for
            expression by an alternative RNA splicing mechanism
  JOURNAL   Proc Natl Acad Sci U S A 82 (23), 7835-7839 (1985)
   PUBMED   3865200
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC037576.1 and AC008894.9.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Dec 19, 2018 this sequence version replaced NM_003290.2.
            
            Summary: This gene encodes a member of the tropomyosin family of
            actin-binding proteins involved in the contractile system of
            striated and smooth muscles and the cytoskeleton of non-muscle
            cells. Tropomyosins are dimers of coiled-coil proteins that
            polymerize end-to-end along the major groove in most actin
            filaments. They provide stability to the filaments and regulate
            access of other actin-binding proteins. In muscle cells, they
            regulate muscle contraction by controlling the binding of myosin
            heads to the actin filament. Multiple transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Nov 2009].
            
            Transcript Variant: This variant (Tpm4.2, also known as variant 2)
            differs in the 5' UTR and coding sequence compared to variant
            Tpm4.1. The resulting isoform (Tpm4.2cy, also known as isoform 2)
            has a shorter and distinct N-terminus compared to isoform Tpm4.1cy.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.108830.1,
                                           SRR3476690.1123291.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000643579.2/ ENSP00000495347.1
            RefSeq Select criteria :: based on manual assertion, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1661              BC037576.1         46-1706
            1662-2474           AC008894.9         61457-62269
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.12-p13.11"
     gene            1..2474
                     /gene="TPM4"
                     /gene_synonym="BDPLT25; HEL-S-108"
                     /note="tropomyosin 4"
                     /db_xref="GeneID:7171"
                     /db_xref="HGNC:HGNC:12013"
                     /db_xref="MIM:600317"
     exon            1..203
                     /gene="TPM4"
                     /gene_synonym="BDPLT25; HEL-S-108"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..818
                     /gene="TPM4"
                     /gene_synonym="BDPLT25; HEL-S-108"
                     /note="isoform Tpm4.2cy is encoded by transcript variant
                     1; tropomyosin alpha-4 chain; TM30p1; epididymis secretory
                     protein Li 108"
                     /codon_start=1
                     /product="tropomyosin alpha-4 chain isoform Tpm4.2cy"
                     /protein_id="NP_003281.1"
                     /db_xref="CCDS:CCDS12338.1"
                     /db_xref="GeneID:7171"
                     /db_xref="HGNC:HGNC:12013"
                     /db_xref="MIM:600317"
                     /translation="MAGLNSLEAVKRKIQALQQQADEAEDRAQGLQRELDGERERREK
                     AEGDVAALNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRAMKDE
                     EKMEIQEMQLKEAKHIAEEADRKYEEVARKLVILEGELERAEERAEVSELKCGDLEEE
                     LKNVTNNLKSLEAASEKYSEKEDKYEEEIKLLSDKLKEAETRAEFAERTVAKLEKTID
                     DLEEKLAQAKEENVGLHQTLDQTLNELNCI"
     exon            204..337
                     /gene="TPM4"
                     /gene_synonym="BDPLT25; HEL-S-108"
                     /inference="alignment:Splign:2.1.0"
     exon            338..455
                     /gene="TPM4"
                     /gene_synonym="BDPLT25; HEL-S-108"
                     /inference="alignment:Splign:2.1.0"
     exon            456..526
                     /gene="TPM4"
                     /gene_synonym="BDPLT25; HEL-S-108"
                     /inference="alignment:Splign:2.1.0"
     exon            527..602
                     /gene="TPM4"
                     /gene_synonym="BDPLT25; HEL-S-108"
                     /inference="alignment:Splign:2.1.0"
     exon            603..665
                     /gene="TPM4"
                     /gene_synonym="BDPLT25; HEL-S-108"
                     /inference="alignment:Splign:2.1.0"
     exon            666..735
                     /gene="TPM4"
                     /gene_synonym="BDPLT25; HEL-S-108"
                     /inference="alignment:Splign:2.1.0"
     exon            736..2474
                     /gene="TPM4"
                     /gene_synonym="BDPLT25; HEL-S-108"
                     /inference="alignment:Splign:2.1.0"
     regulatory      942..947
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPM4"
                     /gene_synonym="BDPLT25; HEL-S-108"
                     /note="hexamer: AATACA"
     polyA_site      969
                     /gene="TPM4"
                     /gene_synonym="BDPLT25; HEL-S-108"
                     /note="major polyA site"
     regulatory      1194..1199
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPM4"
                     /gene_synonym="BDPLT25; HEL-S-108"
                     /note="hexamer: ATTAAA"
     polyA_site      1215
                     /gene="TPM4"
                     /gene_synonym="BDPLT25; HEL-S-108"
     regulatory      2382..2387
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPM4"
                     /gene_synonym="BDPLT25; HEL-S-108"
                     /note="hexamer: AATAAA"
     polyA_site      2415
                     /gene="TPM4"
                     /gene_synonym="BDPLT25; HEL-S-108"
     regulatory      2454..2459
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPM4"
                     /gene_synonym="BDPLT25; HEL-S-108"
                     /note="hexamer: AATAAA"
     polyA_site      2474
                     /gene="TPM4"
                     /gene_synonym="BDPLT25; HEL-S-108"
ORIGIN      
        1 gtgcagctct cgccggagcc gagcccagcc gagcgtccgc cgctgcccgt gcgcctctgc
       61 gcctccgcgc catggccggc ctcaactccc tggaggcggt gaaacgcaag atccaggccc
      121 tgcagcagca ggcggacgag gcggaagacc gcgcgcaggg cctgcagcgg gagctggacg
      181 gcgagcgcga gcggcgcgag aaagctgaag gtgatgtggc cgccctcaac cgacgcatcc
      241 agctcgttga ggaggagttg gacagggctc aggaacgact ggccacggcc ctgcagaagc
      301 tggaggaggc agaaaaagct gcagatgaga gtgagagagg aatgaaggtg atagaaaacc
      361 gggccatgaa ggatgaggag aagatggaga ttcaggagat gcagctcaaa gaggccaagc
      421 acattgcgga agaggctgac cgcaaatacg aggaggtagc tcgtaagctg gtcatcctgg
      481 agggtgagct ggagagggca gaggagcgtg cggaggtgtc tgaactaaaa tgtggtgacc
      541 tggaagaaga actcaagaat gttactaaca atctgaaatc tctggaggct gcatctgaaa
      601 agtattctga aaaggaggac aaatatgaag aagaaattaa acttctgtct gacaaactga
      661 aagaggctga gacccgtgct gaatttgcag agagaacggt tgcaaaactg gaaaagacaa
      721 ttgatgacct ggaagagaaa cttgcccagg ccaaagaaga gaacgtgggc ttacatcaga
      781 cactggatca gacactaaac gaacttaact gtatataagc aaaacagaag agtcttgttc
      841 caacagaaac tctggagctc cgtgggtctt tctcttctct tgtaagaagt tccttttgtt
      901 attgccatct tcgctttgct ggaaatgtca agcaaattat gaatacatga ccaaatattt
      961 tgtatcggag aagctttgag caccagttaa atctcattcc ttcccttttt ttttcaaatg
     1021 gcaccagctt tttcagctct cttatttttt ccttaagtag catttattcc taaggtaggc
     1081 agggtatttc ctagtaagca tactttctta agacggaggc catttggttc ctgggagaat
     1141 aggcagcccc acactttgaa gaatacagac cccagtatct agtcgtggat ataattaaaa
     1201 cgctgaagac cataaccttt tgggtcaact gttggtcaaa ctataggaga gaccagggac
     1261 catcacatgg gtagggattt tccatccaga gccaataaaa ggactggtgg gggccggggg
     1321 tggctattgt gggaagtcat aacccacaga tagatcaacc taagaatcct ggcccttctc
     1381 cactctccac catgcaggac aaacatcttc tcaagcagtc aacgtagaat gcttgggaaa
     1441 tagtcataat tacccacata tagtaattaa tagatggtaa ttaattgatc cttgatgtga
     1501 tgttcttttg catatttcct tcattctaaa gttgttccct ggccgggagc gttggctttc
     1561 gcctgtaatc ccaacacttt gggaggccag gacagatcac ttgaggtcag gagttcgaga
     1621 ccagcccagc caacatggcg aaaccatgtc tctactaaaa atacaaaaat tatggtgacg
     1681 cctgcctgta gtcccagcta ctcgggaggc tgaggcagga ggatcgcttg aacccaggaa
     1741 gtggagactg cagtgagccg atatcgcacc acagcgctcc agcctggtcg acagagtgag
     1801 actccatctc aagaaaaaat aaaaataaag ttgttctctg aagagcaaat gtctcattcc
     1861 agtaatgacc cactcagcag gaatatggtg gagttcagtc caattcaggt cagccatatc
     1921 caaaagacca caagtcatta ctaagttgag caaaagagtt tttatctatt agcagaaagg
     1981 gcctctctgg cagcagagat taaaaactgg cccaacttca tttccatact tcagggaaca
     2041 gcaaattgag gatttactta tctaggactt gaattccttc tttgggacca agttaataaa
     2101 agaccaagaa actcctgatt aaactggata atgaaggatt ctgtagacag ggctgcacgt
     2161 atcggctttg tttgacttct cttttctcag ttaacatctc agagctagaa cattccacat
     2221 tccccagcag cgtgtggggg ctgactaaag tttacaattc caactaaaaa tcaccctgct
     2281 tctggcttat ctgaatccct tacccacccc accccaccac cctactccta tttattcagc
     2341 accacactac ccaggaaata cactagcaaa ttgtgcaatg gaataaaatc cacactttag
     2401 attcttgcaa ctgtatcata tgtaatagta tcactttttc tacattttgg tcaaataaat
     2461 ttttacataa acta
//
