LOCUS       NM_139075               4969 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens two pore segment channel 2 (TPCN2), mRNA.
ACCESSION   NM_139075
VERSION     NM_139075.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Netcharoensirisuk,P., Abrahamian,C., Tang,R., Chen,C.C.,
            Rosato,A.S., Beyers,W., Chao,Y.K., Filippini,A., Di Pietro,S.,
            Bartel,K., Biel,M., Vollmar,A.M., Umehara,K., De-Eknamkul,W. and
            Grimm,C.
  TITLE     Flavonoids increase melanin production and reduce proliferation,
            migration and invasion of melanoma cells by blocking
            endolysosomal/melanosomal TPC2
  JOURNAL   Sci Rep 11 (1), 8515 (2021)
   PUBMED   33875769
  REMARK    GeneRIF: Flavonoids increase melanin production and reduce
            proliferation, migration and invasion of melanoma cells by blocking
            endolysosomal/melanosomal TPC2.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Bock,J., Krogsaeter,E., Passon,M., Chao,Y.K., Sharma,S.,
            Grallert,H., Peters,A. and Grimm,C.
  TITLE     Human genome diversity data reveal that L564P is the predominant
            TPC2 variant and a prerequisite for the blond hair associated M484L
            gain-of-function effect
  JOURNAL   PLoS Genet 17 (1), e1009236 (2021)
   PUBMED   33465068
  REMARK    GeneRIF: Human genome diversity data reveal that L564P is the
            predominant TPC2 variant and a prerequisite for the blond hair
            associated M484L gain-of-function effect.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4969)
  AUTHORS   Ou,X., Liu,Y., Lei,X., Li,P., Mi,D., Ren,L., Guo,L., Guo,R.,
            Chen,T., Hu,J., Xiang,Z., Mu,Z., Chen,X., Chen,J., Hu,K., Jin,Q.,
            Wang,J. and Qian,Z.
  TITLE     Characterization of spike glycoprotein of SARS-CoV-2 on virus entry
            and its immune cross-reactivity with SARS-CoV
  JOURNAL   Nat Commun 11 (1), 1620 (2020)
   PUBMED   32221306
  REMARK    Erratum:[Nat Commun. 2021 Apr 1;12(1):2144. doi:
            10.1038/s41467-021-22614-1.. PMID: 33795662]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4969)
  AUTHORS   Gerndt,S., Chen,C.C., Chao,Y.K., Yuan,Y., Burgstaller,S., Scotto
            Rosato,A., Krogsaeter,E., Urban,N., Jacob,K., Nguyen,O.N.P.,
            Miller,M.T., Keller,M., Vollmar,A.M., Gudermann,T., Zierler,S.,
            Schredelseker,J., Schaefer,M., Biel,M., Malli,R., Wahl-Schott,C.,
            Bracher,F., Patel,S. and Grimm,C.
  TITLE     Agonist-mediated switching of ion selectivity in TPC2
            differentially promotes lysosomal function
  JOURNAL   Elife 9, e54712 (2020)
   PUBMED   32167471
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4969)
  AUTHORS   She,J., Zeng,W., Guo,J., Chen,Q., Bai,X.C. and Jiang,Y.
  TITLE     Structural mechanisms of phospholipid activation of the human TPC2
            channel
  JOURNAL   Elife 8, e45222 (2019)
   PUBMED   30860481
  REMARK    GeneRIF: cryo-EM structure of human TPC2 provides insights into the
            mechanism of PI(3,5)P2-regulated gating of TPC2, which is distinct
            from that of TPC1
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4969)
  AUTHORS   Calcraft,P.J., Ruas,M., Pan,Z., Cheng,X., Arredouani,A., Hao,X.,
            Tang,J., Rietdorf,K., Teboul,L., Chuang,K.T., Lin,P., Xiao,R.,
            Wang,C., Zhu,Y., Lin,Y., Wyatt,C.N., Parrington,J., Ma,J.,
            Evans,A.M., Galione,A. and Zhu,M.X.
  TITLE     NAADP mobilizes calcium from acidic organelles through two-pore
            channels
  JOURNAL   Nature 459 (7246), 596-600 (2009)
   PUBMED   19387438
REFERENCE   7  (bases 1 to 4969)
  AUTHORS   Sulem,P., Gudbjartsson,D.F., Stacey,S.N., Helgason,A., Rafnar,T.,
            Jakobsdottir,M., Steinberg,S., Gudjonsson,S.A., Palsson,A.,
            Thorleifsson,G., Palsson,S., Sigurgeirsson,B., Thorisdottir,K.,
            Ragnarsson,R., Benediktsdottir,K.R., Aben,K.K., Vermeulen,S.H.,
            Goldstein,A.M., Tucker,M.A., Kiemeney,L.A., Olafsson,J.H.,
            Gulcher,J., Kong,A., Thorsteinsdottir,U. and Stefansson,K.
  TITLE     Two newly identified genetic determinants of pigmentation in
            Europeans
  JOURNAL   Nat Genet 40 (7), 835-837 (2008)
   PUBMED   18488028
  REMARK    GeneRIF: Two coding variants in TPCN2 are associated with hair
            color, and a variant at the ASIP locus shows strong association
            with skin sensitivity to sun, freckling and red hair.
            GeneRIF: Genome-wide association study of gene-disease association.
            (HuGE Navigator)
REFERENCE   8  (bases 1 to 4969)
  AUTHORS   Taylor,T.D., Noguchi,H., Totoki,Y., Toyoda,A., Kuroki,Y., Dewar,K.,
            Lloyd,C., Itoh,T., Takeda,T., Kim,D.W., She,X., Barlow,K.F.,
            Bloom,T., Bruford,E., Chang,J.L., Cuomo,C.A., Eichler,E.,
            FitzGerald,M.G., Jaffe,D.B., LaButti,K., Nicol,R., Park,H.S.,
            Seaman,C., Sougnez,C., Yang,X., Zimmer,A.R., Zody,M.C.,
            Birren,B.W., Nusbaum,C., Fujiyama,A., Hattori,M., Rogers,J.,
            Lander,E.S. and Sakaki,Y.
  TITLE     Human chromosome 11 DNA sequence and analysis including novel gene
            identification
  JOURNAL   Nature 440 (7083), 497-500 (2006)
   PUBMED   16554811
REFERENCE   9  (bases 1 to 4969)
  AUTHORS   Clapham,D.E. and Garbers,D.L.
  TITLE     International Union of Pharmacology. L. Nomenclature and
            structure-function relationships of CatSper and two-pore channels
  JOURNAL   Pharmacol Rev 57 (4), 451-454 (2005)
   PUBMED   16382101
  REMARK    Review article
REFERENCE   10 (bases 1 to 4969)
  AUTHORS   Fu,G.K., Wang,J.T., Yang,J., Au-Young,J. and Stuve,L.L.
  TITLE     Circular rapid amplification of cDNA ends for high-throughput
            extension cloning of partial genes
  JOURNAL   Genomics 84 (1), 205-210 (2004)
   PUBMED   15203218
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP003071.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_139075.3.
            
            Summary: This gene encodes a putative cation-selective ion channel
            with two repeats of a six-transmembrane-domain. The protein
            localizes to lysosomal membranes and enables nicotinic acid adenine
            dinucleotide phosphate (NAADP) -induced calcium ion release from
            lysosome-related stores. This ubiquitously expressed gene has
            elevated expression in liver and kidney. Two common nonsynonymous
            SNPs in this gene strongly associate with blond versus brown hair
            pigmentation.[provided by RefSeq, Dec 2009].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY029200.1, BC063008.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000294309.8/ ENSP00000294309.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-175               AP003071.3         63426-63600
            176-240             AP003071.3         68527-68591
            241-317             AP003071.3         69215-69291
            318-495             AP003071.3         69669-69846
            496-612             AP003071.3         72072-72188
            613-719             AP003071.3         77378-77484
            720-792             AP003071.3         78389-78461
            793-895             AP003071.3         81997-82099
            896-961             AP003071.3         84924-84989
            962-1026            AP003071.3         85850-85914
            1027-1127           AP003071.3         86417-86517
            1128-1209           AP003071.3         87121-87202
            1210-1296           AP003071.3         87409-87495
            1297-1416           AP003071.3         92976-93095
            1417-1476           AP003071.3         93228-93287
            1477-1605           AP003071.3         93386-93514
            1606-1655           AP003071.3         94328-94377
            1656-1755           AP003071.3         95894-95993
            1756-1827           AP003071.3         98439-98510
            1828-1904           AP003071.3         99704-99780
            1905-1986           AP003071.3         100165-100246
            1987-2069           AP003071.3         100342-100424
            2070-2151           AP003071.3         101017-101098
            2152-2246           AP003071.3         101606-101700
            2247-4969           AP003071.3         102369-105091
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.3"
     gene            1..4969
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /note="two pore segment channel 2"
                     /db_xref="GeneID:219931"
                     /db_xref="HGNC:HGNC:20820"
                     /db_xref="MIM:612163"
     exon            1..175
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             67..2325
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /note="two pore calcium channel protein 2;
                     voltage-dependent calcium channel protein TPC2"
                     /codon_start=1
                     /product="two pore channel protein 2"
                     /protein_id="NP_620714.2"
                     /db_xref="CCDS:CCDS8189.1"
                     /db_xref="GeneID:219931"
                     /db_xref="HGNC:HGNC:20820"
                     /db_xref="MIM:612163"
                     /translation="MAEPQAESEPLLGGARGGGGDWPAGLTTYRSIQVGPGAAARWDL
                     CIDQAVVFIEDAIQYRSINHRVDASSMWLYRRYYSNVCQRTLSFTIFLILFLAFIETP
                     SSLTSTADVRYRAAPWEPPCGLTESVEVLCLLVFAADLSVKGYLFGWAHFQKNLWLLG
                     YLVVLVVSLVDWTVSLSLVCHEPLRIRRLLRPFFLLQNSSMMKKTLKCIRWSLPEMAS
                     VGLLLAIHLCLFTMFGMLLFAGGKQDDGQDRERLTYFQNLPESLTSLLVLLTTANNPD
                     VMIPAYSKNRAYAIFFIVFTVIGSLFLMNLLTAIIYSQFRGYLMKSLQTSLFRRRLGT
                     RAAFEVLSSMVGEGGAFPQAVGVKPQNLLQVLQKVQLDSSHKQAMMEKVRSYGSVLLS
                     AEEFQKLFNELDRSVVKEHPPRPEYQSPFLQSAQFLFGHYYFDYLGNLIALANLVSIC
                     VFLVLDADVLPAERDDFILGILNCVFIVYYLLEMLLKVFALGLRGYLSYPSNVFDGLL
                     TVVLLVLEISTLAVYRLPHPGWRPEMVGLLSLWDMTRMLNMLIVFRFLRIIPSMKLMA
                     VVASTVLGLVQNMRAFGGILVVVYYVFAIIGINLFRGVIVALPGNSSLAPANGSAPCG
                     SFEQLEYWANNFDDFAAALVTLWNLMVVNNWQVFLDAYRRYSGPWSKIYFVLWWLVSS
                     VIWVNLFLALILENFLHKWDPRSHLQPLAGTPEATYQMTVELLFRDILEEPGEDELTE
                     RLSQHPHLWLCR"
     exon            176..240
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            241..317
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            318..495
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            496..612
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            613..719
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            720..792
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            793..895
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            896..961
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1026
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1127
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1128..1209
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1296
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1297..1416
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1476
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1605
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1606..1655
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1755
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1827
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1828..1904
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..1986
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1987..2069
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2070..2151
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2152..2246
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2247..4969
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2857..2862
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /note="hexamer: AATAAG"
     polyA_site      2874
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /note="major polyA site"
     regulatory      3681..3686
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /note="hexamer: AGTAAA"
     polyA_site      3703
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
     regulatory      4944..4949
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
                     /note="hexamer: AATAAA"
     polyA_site      4969
                     /gene="TPCN2"
                     /gene_synonym="SHEP10; TPC2"
ORIGIN      
        1 gcagtgaagc tgggcgcctt cggggcttga gcttctgagg gtcgggtcca gcgcgtgggc
       61 tgctggatgg cggaacccca ggcggagtcg gagcccctgc tgggcggggc ccgcggcggt
      121 ggcggcgact ggccggcggg gctgaccact taccgcagca tccaagtcgg ccctggtgcc
      181 gcggccaggt gggacctctg cattgatcag gctgtggtct tcatcgaaga tgctattcag
      241 taccgctcca tcaaccaccg ggtggatgcc agctcgatgt ggctttaccg acggtattac
      301 tcgaacgtat gccaacggac tttgagcttc accatcttct tgatcctgtt tttggctttt
      361 atcgagaccc catcctcact caccagcacg gcggacgtgc gctaccgcgc tgctccctgg
      421 gagccgccct gcggcctgac cgagagtgtc gaggtgctct gcctgctggt ctttgcggcc
      481 gacctctctg tgaagggtta cctgttcggg tgggcccatt tccagaaaaa cctttggctg
      541 ctgggctacc tcgtggtgct ggtggtgtct ctggtggact ggaccgtgtc cctgagtctc
      601 gtgtgtcatg agcccctgcg gatccgccgg cttctccgtc ccttcttcct gctgcagaac
      661 tcctctatga tgaagaagac cttgaaatgc atccgctggt cgctgccgga aatggccagc
      721 gtcgggctgc tgctggccat ccacctgtgc ctcttcacca tgttcggaat gctgctgttc
      781 gctggtggga agcaggatga tgggcaggac agggagaggc tgacctactt ccagaacctg
      841 cctgagtctc tgacttccct cctggtgctg ctgaccacgg ccaacaaccc cgatgtgatg
      901 attcctgcgt attccaagaa ccgggcctat gccatcttct tcatagtctt cactgtgata
      961 ggaagcctgt ttctgatgaa cctgctgaca gccatcatct acagtcagtt ccggggctac
     1021 ctgatgaaat ctctccagac ctcgctgttt cggaggcggc tgggaacccg ggctgccttt
     1081 gaagtcctat cctccatggt gggggaggga ggagccttcc ctcaggcagt tggggtgaag
     1141 ccccagaact tgctgcaggt gcttcagaag gtccagctgg acagctccca caaacaggcc
     1201 atgatggaga aggtgcgttc ctatggcagt gttctgctct cagctgagga gtttcagaag
     1261 ctcttcaacg agcttgacag aagtgtggtt aaagagcacc cgccgaggcc cgagtaccag
     1321 tctccgtttc tgcagagcgc ccagttcctc ttcggccact actactttga ctacctgggg
     1381 aacctcatcg ccctggcaaa cctggtgtcc atttgcgtgt tcctggtgct ggatgcagat
     1441 gtgctgcctg ctgagcgtga tgacttcatc ctggggattc tcaactgcgt cttcattgtg
     1501 tactacctgt tggagatgct gctcaaggtc tttgccctgg gcctgcgagg gtacctgtcc
     1561 taccccagca acgtgtttga cgggctcctc accgttgtcc tgctggtttt ggagatctca
     1621 actctggctg tgtaccgatt gccacaccca ggctggaggc cggagatggt gggcctgctg
     1681 tcgctgtggg acatgacccg catgctgaac atgctcatcg tgttccgctt cctgcgtatc
     1741 atccccagca tgaagctgat ggccgtggtg gccagtaccg tcctgggcct ggtgcagaac
     1801 atgcgtgcgt ttggcgggat cctggtggtg gtctactacg tatttgccat cattgggatc
     1861 aacttgttta gaggcgtcat tgtggctctt cctggaaaca gcagcctggc ccctgccaat
     1921 ggctcggcgc cctgtgggag cttcgagcag ctggagtact gggccaacaa cttcgatgac
     1981 tttgcggctg ccctggtcac tctgtggaac ttgatggtgg tgaacaactg gcaggtgttt
     2041 ctggatgcat atcggcgcta ctcaggcccg tggtccaaga tctattttgt attgtggtgg
     2101 ctggtgtcgt ctgtcatctg ggtcaacctg tttctggccc tgattctgga gaacttcctt
     2161 cacaagtggg acccccgcag ccacctgcag ccccttgctg ggaccccaga ggccacctac
     2221 cagatgactg tggagctcct gttcagggat attctggagg agcccgggga ggatgagctc
     2281 acagagaggc tgagccagca cccgcacctg tggctgtgca ggtgacgtcc gggctgccgt
     2341 cccagcaggg gcggcaggag agagaggctg gcctacacag gtgcccgtca tggaagaggc
     2401 ggccatgctg tggccagcca ggcaggaaga gacctttcct ctgacggacc actaagctgg
     2461 ggacaggaac caagtccttt gcgtgtggcc caacaaccat ctacagaaca gctgctggtg
     2521 cttcagggag gcgccgtgcc ctccgctttc ttttatagct gcttcagtga gaattccctc
     2581 gtcgactcca cagggacctt tcagacaaaa atgcaagaag cagcggcctc ccctgtcccc
     2641 tgcagcttcc gtggtgcctt tgctgccggc agcccttggg gaccacaggc ctgaccaggg
     2701 cctgcacagg ttaaccgtca gacttccggg gcattcaggt ggggatgctg gtggtttgac
     2761 atggagagaa ccttgactgt gttttattat ttcatggctt gtatgagtgt gactgggtgt
     2821 gtttctttag ggttctgatt gccagttatt ttcatcaata agtcttgcaa agaatgggat
     2881 tgtcattctt cacttcagca cagttctagt cctgcttctc tggagtaggg ttgttgagta
     2941 aggttgcttg ggttgtgcat tgcacaaggg cacatggctg tgaggtgtat cctggcgggg
     3001 ggctgtctac ctgcagtgag gggcaccttt tctgttttgc tcaaaggcat gtataagcca
     3061 atgggtgacc ttatttcctg tgtcttcagg tgtgtggcag ggggcctggg gtggggaggt
     3121 ggggcgagcg agcagtgtgt ggaaagcctt gttgtcacct gaagcacgcc aggtccagat
     3181 tgaccaatgg ttttctcact tcaggggcca acccacgccc cctttctgct gaggtttggg
     3241 tgccatctag tggtgggatg ggacttggtt gactacattt aaggtaaggt ggacccagca
     3301 actcccagaa acaactccgg ggacaccact ccccatcaca ctccacaccg agcctggtgc
     3361 ccggtctgtg cccgagctca gcgggaccag gaagggatgg gccctgccag ggttgcccct
     3421 gcactgtgca ttctcgcctg ggaggcacaa gttctttcat ctgcttttcc ttcagaggtg
     3481 ctgagcccac gccatagccc ctgtgggatg gtgggggagg gggcgacccg aacaacagtg
     3541 cagtcggtat cgagattggg gagaggagcg agtccaagga gaaggtcatg agtttctttt
     3601 tactcgtgtt gaataataac aataacaata acaataacaa tatggaaacc accgcaaact
     3661 tggagaaaag ttgtaagcac agtaaagaga agcttccttc tgagtcactt gagtggttgc
     3721 cgttctggcc ctgcaccctc tgtgctttgg gacggcgtcc aacccgcatt catgtcagga
     3781 gtgagtcgca cgtggctttg tggtcatggc gacttaatcc gcctggacgg tggctccgtc
     3841 tccctgggct tagacgacct tggcacttct ggagataagc ccatggctcc caggttgtgt
     3901 tcatgtgacg tttccttgtg gtaggttctg ggtctgcgtt tcgtctagga gtgtcacagg
     3961 atggacactg cctcctggca ggggctgccc aatgcagtta gcctcctgct ggtgttctct
     4021 cttgttgctt ggtgaaggtg gccctggtca gcttctccac tgcccagtga acgacccctt
     4081 tgtaatgaat gagtggggag gtagtgtgaa gcgatgccaa tatcccatcc ctgtcaaact
     4141 gcctttactt tttccttcct tccttgctcc cacctgtgtg gatcctggtc ccttcttgta
     4201 ttcagggctg tggtctgtta tgacatttac tctcaggctc aggtcctgct tgtttggccc
     4261 gtgggagccc cttcttctgc cttttgtgtt ttttttgtta tgtacctaca gttctttagc
     4321 tgtttcttta ctttctggtg caaaaagatg ttcaagcctt attttatact tgcctgcccc
     4381 tttctctttc atttattgga gtgagctgca gctctaagaa gacctgttct tttgaatgga
     4441 gagtagcatc aggaaccagg atgtgggtgc gaggcgtgct cctggctgtt gcagattgct
     4501 gcacccggga gctcttagtg gacagagcta gaggatatgt gcacgtactt ccatctctct
     4561 ctctgtctca tctattcaca gctgggaatg atactaatac ctccgatttt agcccagcac
     4621 cacagggtac gttccagttt ttctctcttt ccatagctgt aaggcccttt ctgggaatgg
     4681 ttctcattct ccttaatcta ttattgggtc agttttcctg catgtcccca gcctcccatc
     4741 actgccaccc actccccaca gagatgccct gctcatccga ctggggcttt gactcccaca
     4801 ctgtgtaccc ctcttgtgtg gacgccctgc tgccaaaacc ttcagcaaac agctttccaa
     4861 atggaagttg tcactgtcag ggcctttaca atcagcaaca gcaaaatcta catgctgctg
     4921 agggtcctgc ctcattaaga tgcaataaat atgtaagtac ataaaaaca
//
