LOCUS       NM_017901               5248 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens two pore segment channel 1 (TPCN1), transcript variant
            2, mRNA.
ACCESSION   NM_017901
VERSION     NM_017901.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Trufanov,S.K., Rybakova,E.Y., Avdonin,P.P., Tsitrina,A.A.,
            Zharkikh,I.L., Goncharov,N.V., Jenkins,R.O. and Avdonin,P.V.
  TITLE     The Role of Two-Pore Channels in Norepinephrine-Induced [Ca2+]i
            Rise in Rat Aortic Smooth Muscle Cells and Aorta Contraction
  JOURNAL   Cells 8 (10), 1144 (2019)
   PUBMED   31557916
  REMARK    GeneRIF: TPC1 is involved in the norepinephrine (NE)-stimulated
            [Ca(2+)]i rise in Smooth muscle cells. Inhibition of TPC1 activity
            by NED 19 could be the reason for partial inhibition of aortic
            rings contraction in response to NE.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   She,J., Zeng,W., Guo,J., Chen,Q., Bai,X.C. and Jiang,Y.
  TITLE     Structural mechanisms of phospholipid activation of the human TPC2
            channel
  JOURNAL   Elife 8, e45222 (2019)
   PUBMED   30860481
  REMARK    GeneRIF: cryo-EM structure of human TPC2 provides insights into the
            mechanism of PI(3,5)P2-regulated gating of TPC2, which is distinct
            from that of TPC1
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5248)
  AUTHORS   Patel,S., Churamani,D. and Brailoiu,E.
  TITLE     NAADP-evoked Ca2+ signals through two-pore channel-1 require
            arginine residues in the first S4-S5 linker
  JOURNAL   Cell Calcium 68, 1-4 (2017)
   PUBMED   29129203
  REMARK    GeneRIF: a cluster of arginine residues in the first domain
            required for selective voltage-gating of TPC1 map not to the
            voltage-sensing fourth transmembrane region (S4) but to a cytosolic
            downstream region (S4-S5 linker).
REFERENCE   4  (bases 1 to 5248)
  AUTHORS   Lagostena,L., Festa,M., Pusch,M. and Carpaneto,A.
  TITLE     The human two-pore channel 1 is modulated by cytosolic and luminal
            calcium
  JOURNAL   Sci Rep 7, 43900 (2017)
   PUBMED   28252105
  REMARK    GeneRIF: These data point to a process in which Ca(2+) permeation
            in human TPC1 has a positive feedback on channel activity while
            Na(+) acts as a negative regulator.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5248)
  AUTHORS   Jahidin,A.H., Stewart,T.A., Thompson,E.W., Roberts-Thomson,S.J. and
            Monteith,G.R.
  TITLE     Differential effects of two-pore channel protein 1 and 2 silencing
            in MDA-MB-468 breast cancer cells
  JOURNAL   Biochem Biophys Res Commun 477 (4), 731-736 (2016)
   PUBMED   27353380
  REMARK    GeneRIF: These findings indicate potential differential regulation
            of signaling processes by TPC1 and TPC2 in breast cancer cells.
REFERENCE   6  (bases 1 to 5248)
  AUTHORS   Brailoiu,E., Churamani,D., Cai,X., Schrlau,M.G., Brailoiu,G.C.,
            Gao,X., Hooper,R., Boulware,M.J., Dun,N.J., Marchant,J.S. and
            Patel,S.
  TITLE     Essential requirement for two-pore channel 1 in NAADP-mediated
            calcium signaling
  JOURNAL   J Cell Biol 186 (2), 201-209 (2009)
   PUBMED   19620632
  REMARK    GeneRIF: Data show that human two-pore channel TPC1 is critical for
            nicotinic acid adenine dinucleotide phosphate action and is likely
            the long sought after target channel for NAADP.
REFERENCE   7  (bases 1 to 5248)
  AUTHORS   Calcraft,P.J., Ruas,M., Pan,Z., Cheng,X., Arredouani,A., Hao,X.,
            Tang,J., Rietdorf,K., Teboul,L., Chuang,K.T., Lin,P., Xiao,R.,
            Wang,C., Zhu,Y., Lin,Y., Wyatt,C.N., Parrington,J., Ma,J.,
            Evans,A.M., Galione,A. and Zhu,M.X.
  TITLE     NAADP mobilizes calcium from acidic organelles through two-pore
            channels
  JOURNAL   Nature 459 (7246), 596-600 (2009)
   PUBMED   19387438
REFERENCE   8  (bases 1 to 5248)
  AUTHORS   Clapham,D.E. and Garbers,D.L.
  TITLE     International Union of Pharmacology. L. Nomenclature and
            structure-function relationships of CatSper and two-pore channels
  JOURNAL   Pharmacol Rev 57 (4), 451-454 (2005)
   PUBMED   16382101
  REMARK    Review article
REFERENCE   9  (bases 1 to 5248)
  AUTHORS   Zhou,G., Wang,J., Zhang,Y., Zhong,C., Ni,J., Wang,L., Guo,J.,
            Zhang,K., Yu,L. and Zhao,S.
  TITLE     Cloning, expression and subcellular localization of HN1 and HN1L
            genes, as well as characterization of their orthologs, defining an
            evolutionarily conserved gene family
  JOURNAL   Gene 331, 115-123 (2004)
   PUBMED   15094197
REFERENCE   10 (bases 1 to 5248)
  AUTHORS   Ishibashi,K., Suzuki,M. and Imai,M.
  TITLE     Molecular cloning of a novel form (two-repeat) protein related to
            voltage-gated sodium and calcium channels
  JOURNAL   Biochem Biophys Res Commun 270 (2), 370-376 (2000)
   PUBMED   10753632
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK093490.1 and BC150203.1.
            
            On Jun 2, 2019 this sequence version replaced NM_017901.5.
            
            Summary: Voltage-gated Ca(2+) and Na+ channels have 4 homologous
            domains, each containing 6 transmembrane segments, S1 to S6. TPCN1
            is similar to these channels, but it has only 2 domains containing
            S1 to S6 (Ishibashi et al., 2000 [PubMed 10753632]).[supplied by
            OMIM, Mar 2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and uses a downstream start codon,
            compared to variant 1. The encoded isoform (2) has a shorter
            N-terminus compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC150203.1, AB032995.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000335509.11/ ENSP00000335300.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-51                AK093490.1         27-77
            52-5248             BC150203.1         1-5197
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.13"
     gene            1..5248
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /note="two pore segment channel 1"
                     /db_xref="GeneID:53373"
                     /db_xref="HGNC:HGNC:18182"
                     /db_xref="MIM:609666"
     exon            1..163
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            164..400
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             289..2739
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /note="isoform 2 is encoded by transcript variant 2; two
                     pore calcium channel protein 1; voltage-dependent calcium
                     channel protein TPC1; two-pore channel 1, homolog; two
                     pore channel protein 1"
                     /codon_start=1
                     /product="two pore channel protein 1 isoform 2"
                     /protein_id="NP_060371.2"
                     /db_xref="CCDS:CCDS31908.1"
                     /db_xref="GeneID:53373"
                     /db_xref="HGNC:HGNC:18182"
                     /db_xref="MIM:609666"
                     /translation="MAVSLDDDVPLILTLDEGGSAPLAPSNGLGQEELPSKNGGSYAI
                     HDSQAPSLSSGGESSPSSPAHNWEMNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLF
                     AHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLR
                     WLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGV
                     RRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVL
                     LTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSL
                     LLHKRTAIQHAYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTP
                     LLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLV
                     VAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWN
                     LFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTMFELLPR
                     MASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGY
                     YYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTII
                     VAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRL
                     LETLSQMERYQQHSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQRQLSSSA
                     APAAQQPPGSRQRSQTVT"
     exon            401..525
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            526..702
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            703..816
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            817..947
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1036
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1071
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1130
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1131..1230
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1347
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1348..1472
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..1521
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1522..1585
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1586..1630
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1631..1687
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..1741
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1742..1814
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1815..1922
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1923..1994
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1995..2084
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2085..2200
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2201..2247
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2248..2316
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2317..2401
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2402..2541
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2542..2622
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2623..5248
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5224..5229
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /note="hexamer: ACTAAA"
     polyA_site      5248
                     /gene="TPCN1"
                     /gene_synonym="TPC1"
                     /note="major polyA site"
ORIGIN      
        1 gagctggcgc agctgccctg gtggcagtgg ctgaagtggc ggcggcttcg gcggctgcgg
       61 cggctgcaac agcttcgggc tcggggtttt ggcggcggcg ccggcgggct aggctgcgcg
      121 gtgcggaccc cggcgcgcgg tccgggttgc tggggcggcg cgtaagatgc ctctaatgga
      181 ggagtttctg agcagcaccc ctggcccagt ggctttgaaa gggagctcaa accagagact
      241 atttcaagcc ctggatatca tatcctgagg gccacaggag aagagaacat ggctgtgagt
      301 ttggatgacg acgtgccgct catcctgacc ttggatgagg gtggcagtgc cccactggct
      361 ccctccaacg gcctgggcca agaagagcta cctagcaaaa atggcggcag ctatgccatc
      421 cacgactccc aggcccccag tctcagctct gggggtgaga gttccccctc cagccccgca
      481 cacaactggg agatgaatta ccaagaggca gcaatctacc tccaggaagg cgagaacaac
      541 gacaagttct tcacccaccc caaggatgcc aaggcgctgg cggcctacct ctttgcacac
      601 aatcacctct tctacctgat ggagctggcc acggccctgc tgctgctgct gctctccctg
      661 tgcgaggccc ccgccgtccc cgcactccgg cttggcatct atgtccacgc caccctggag
      721 ctgtttgccc tgatggtggt agtgtttgaa ctctgcatga agttacgctg gctgggcctc
      781 cacaccttca tccggcacaa gcggaccatg gtcaagacct cggtgctggt ggtgcagttt
      841 gtcgaggcca tcgtggtgtt ggtacggcag atgtcccatg tgcgggtgac ccgagcactg
      901 cgctgcattt tcctggtgga ctgtcggtat tgcggtggcg tccggcgcaa cctgcggcag
      961 atcttccagt ccctgccgcc cttcatggac atcctcctgc tgctgctgtt cttcatgatc
     1021 atctttgcca tcctcggttt ctacttgttc tcccctaacc cttcagaccc ctacttcagc
     1081 accctggaga acagcatcgt cagtctgttt gtccttctga ccacagccaa tttcccagat
     1141 gtgatgatgc cctcctactc ccggaacccc tggtcctgcg tcttcttcat cgtgtacctc
     1201 tccatcgagc tgtatttcat catgaacctg cttctggctg tggtgttcga caccttcaat
     1261 gacattgaga aacgcaagtt caagtctttg ctactgcaca agcgaaccgc tatccagcat
     1321 gcctaccgcc tgctcatcag ccagaggagg cctgccggca tctcctacag gcagtttgaa
     1381 ggcctcatgc gcttctacaa gccccggatg agtgccaggg agcgctatct taccttcaag
     1441 gccctgaatc agaacaacac acccctgctc agcctaaagg acttttacga tatctacgaa
     1501 gttgctgctt tgaagtggaa ggccaagaaa aacagagagc actggtttga tgagcttccc
     1561 aggacggcgc tcctcatctt caaaggtatt aatatccttg tgaagtccaa ggccttccag
     1621 tatttcatgt acttggtggt ggcagtcaac ggggtctgga tcctcgtgga gacatttatg
     1681 ctgaaaggtg ggaacttctt ctccaagcac gtgccctgga gttacctcgt ctttctaact
     1741 atctatgggg tggagctgtt cctgaaggtt gccggcctgg gccctgtgga gtacttgtct
     1801 tccggatgga acttgtttga cttctccgtg acagtgttcg ccttcctggg actgctggcg
     1861 ctggccctca acatggagcc cttctatttc atcgtggtcc tgcgccccct ccagctgctg
     1921 aggttgttta agttgaagga gcgctaccgc aacgtgctgg acaccatgtt cgagctgctg
     1981 ccccggatgg ccagcctggg cctcaccctg ctcatctttt actactcctt cgccatcgtg
     2041 ggcatggagt tcttctgcgg gatcgtcttc cccaactgct gcaacacgag tacagtggca
     2101 gatgcctacc gctggcgcaa ccacaccgtg ggcaacagga ccgtggtgga ggaaggctac
     2161 tattatctca ataattttga caacatcctc aacagctttg tgaccctgtt tgagctcaca
     2221 gttgtcaaca actggtacat catcatggaa ggcgtcacct ctcagacctc ccactggagc
     2281 cgcctctact tcatgacctt ttacattgtg accatggtgg tgatgacgat cattgtcgcc
     2341 tttatcctcg aggccttcgt cttccgaatg aactacagcc gcaagaacca ggactcggaa
     2401 gttgatggtg gcatcaccct tgagaaggaa atctccaaag aagagctggt tgccgtcctg
     2461 gagctctacc gggaggcacg gggggcctcc tcggatgtca ccaggctgct ggagaccctc
     2521 tcccagatgg agagatacca gcaacattcc atggtgtttc tgggacggcg atcaaggacc
     2581 aagagcgacc tgagcctgaa gatgtaccag gaggagatcc aggagtggta tgaggagcat
     2641 gccagggagc aagagcagca gcgacaactc agcagcagtg cagcccccgc cgcccagcag
     2701 cccccaggca gccgccagcg ctcccagacc gttacctagc ccagcgcccg aaagccgtct
     2761 cttctatgca ataacacaat agtattactc tactgcgatg tacggaactg cggtgtgtgt
     2821 acacatactc acgtatatgc acatatttat atacaggaag aaaaaagaca gacaagatgg
     2881 ggcttggttt ataaccacct tgccctgtct tccttaactc cagaagccag tttggtgagg
     2941 ggtgggggtg cggccaccag gtctgagctc ttcctactgt ggaaggctcc agaaggccct
     3001 tcacaaggag acccctcacc tggatccagt cgactgcggg gcttgcccct catgtgggct
     3061 ggcctccatc ggccacgtcc aaagctgtca ctgctactgc ttcaggctca catccccccg
     3121 acctgatggc gtgcccgccc cctctccctg cggcccatgc cacaggtttc tgtgttttgc
     3181 tttagggaca gaaccactta ggaaggaaag aactcccggt ctccagggtg gtatttcagt
     3241 gtctgtgata atgtcacgca acacctcttc ggggaccagt gcccaggatc taatggaagc
     3301 ggaattgggg caactgggcc catgtggcca gagctcagtt agccagtgcc gggcggccac
     3361 agattacact gaccaatctc ctcccttggc tctgcaagcc tcccacccag ccttctctgg
     3421 cttaaccctt gttggcgaaa actcttccac agtggcctcc ttggggaccc agaacccgga
     3481 ggaaggggca tgaggcagga agtggggccg atgtctgcaa cccagaccac ttcgtggaat
     3541 gggctcttga ccaaatccct ttttttgcga tttacccgtt caagcaaaac aacgttttgg
     3601 ttaactaagg attgtgctaa agccgatacc aggtccttca cacgtgtgca ctaggaacag
     3661 gagcgaacag cacagagaga cgctccctgt gggacgcagc agccccgtgg ccccggccca
     3721 gttcccagcc accctccctg gctctgctca caccagagat ttccatagca ggagcggttg
     3781 gtgcagaagt aggttcagat gaacctcagt taacgtcgcc acccctcctc ccaccatggt
     3841 accctgtagg agccctgtat gacatctgag cgtggtggag gtaggagggt tgccagctgc
     3901 agtgaccctg ccacagaggc agggtcagtg cagaggtcgc tttggttccg cttccctggg
     3961 ccacagaacg gaacacagca taggttctgc agcaggagcc gcagtggcag gatggagggt
     4021 gcgaagggca aggagtgcac tgctgggcat tcctggccag ccccggccct ctggtgcctg
     4081 cttcctgtga cttcagaagg caggtggaca gagcctccct ctggccttgt cctcttccca
     4141 gccacagaac gggcagggtg gcacccgacc ccaggggagc agtacctggt cccccacccc
     4201 ctcctcccaa ccacctccaa ggccaagctg ggtcccatag ccagcacggc atggttctcc
     4261 ccttcccccc ttcccaggtc aggggagttg gacaagtagc aggtgtttgt ttttaaagca
     4321 cagccctttg ggaaagcaac acattattga gactcactgt gattcccccg ggagtcagac
     4381 tggctttgtc ctcttcctct ctggagggcc atgggccatc agcaggagct ccacatcgag
     4441 ccccaggcca gaaccccctc cctttcacag agagggaact ttattgcaca attgggtgcc
     4501 ttttagcttt tgtgtgttga aatgggcgtt ttggaagcaa gggtcagggg acagcttcta
     4561 aaggtgtgag tctctgacct gagcatctgg gcctcgcctg ggcccttctt cctccccagg
     4621 ggtggaaacg tggaggggcc agcagcaccc cggggtctgc ccaggggagt caaggccccg
     4681 aggtgggggg gcctattcca ggaggagtgg gatctcggcc ctgtccagag cgagttaatg
     4741 tgtccatctg cccaaccctg tcctgagaaa ggacctggtt ttggccagga cctgacaaat
     4801 acccaatggc agcagtgtca acagacggga gtccagccag ggtgggtgcc cttgtcattg
     4861 aggttaggga cagctatccc caggttatgc ctggccccac ccagcaggga gttggggtcc
     4921 ccccacaggc tgtgagctct gtgggccctg ggatgtgatc tagctcagat gccctctcat
     4981 ccttgatgtc atagttgagt gcacccaagt ggcacccact ggcggccagg ggcacagcct
     5041 ggtggtggtg gcaatagaac tgtgcccctc ctcagctcct cgcttcccct cccacagccc
     5101 cagccttact ccaccatgcg gacaatcatt ttgtacggat cacgggagca atgctgtacg
     5161 gttttgtaca ctggtggttt gtttcctaga aaacccattg tgtctctgga tttctagcac
     5221 attactaaaa gagcctctgc tttgtaaa
//
