LOCUS       NM_001376922            2890 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens trophoblast glycoprotein (TPBG), transcript variant 3,
            mRNA.
ACCESSION   NM_001376922
VERSION     NM_001376922.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Su,H., Yu,S., Sun,F., Lin,D., Liu,P. and Zhao,L.
  TITLE     LINC00342 induces metastasis of lung adenocarcinoma by targeting
            miR-15b/TPBG
  JOURNAL   Acta Biochim Pol 69 (2), 291-297 (2022)
   PUBMED   35452205
  REMARK    GeneRIF: LINC00342 induces metastasis of lung adenocarcinoma by
            targeting miR-15b/TPBG.
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Spencer,H.L., Jover,E., Cathery,W., Avolio,E.,
            Rodriguez-Arabaolaza,I., Thomas,A.C., Alvino,V.V., Sala-Newby,G.,
            Dang,Z., Fagnano,M., Reni,C., Rowlinson,J., Vono,R., Spinetti,G.,
            Beltrami,A.P., Gargioli,C., Caporali,A., Angelini,G. and Madeddu,P.
  TITLE     Role of TPBG (Trophoblast Glycoprotein) Antigen in Human Pericyte
            Migratory and Angiogenic Activity
  JOURNAL   Arterioscler Thromb Vasc Biol 39 (6), 1113-1124 (2019)
   PUBMED   31018661
  REMARK    GeneRIF: TPBG orchestrates the migratory and angiogenic activities
            of pericytes through the activation of the CXCL12/CXCR7/pERK axis.
REFERENCE   3  (bases 1 to 2890)
  AUTHORS   Alam,S.M.K., Jasti,S., Kshirsagar,S.K., Tannetta,D.S.,
            Dragovic,R.A., Redman,C.W., Sargent,I.L., Hodes,H.C., Nauser,T.L.,
            Fortes,T., Filler,A.M., Behan,K., Martin,D.R., Fields,T.A.,
            Petroff,B.K. and Petroff,M.G.
  TITLE     Trophoblast Glycoprotein (TPGB/5T4) in Human Placenta: Expression,
            Regulation, and Presence in Extracellular Microvesicles and
            Exosomes
  JOURNAL   Reprod Sci 25 (2), 185-197 (2018)
   PUBMED   28481180
  REMARK    GeneRIF: In extravillous trophoblasts, 5T4 may function in
            epithelial-to-mesenchymal transition during placentation. The role
            of syncytiotrophoblast 5T4 is unknown, but its abundance in shed
            syncytial vesicles may signify route of sensitization of the
            maternal immune system.
REFERENCE   4  (bases 1 to 2890)
  AUTHORS   Jun,G.R., Chung,J., Mez,J., Barber,R., Beecham,G.W., Bennett,D.A.,
            Buxbaum,J.D., Byrd,G.S., Carrasquillo,M.M., Crane,P.K.,
            Cruchaga,C., De Jager,P., Ertekin-Taner,N., Evans,D., Fallin,M.D.,
            Foroud,T.M., Friedland,R.P., Goate,A.M., Graff-Radford,N.R.,
            Hendrie,H., Hall,K.S., Hamilton-Nelson,K.L., Inzelberg,R.,
            Kamboh,M.I., Kauwe,J.S.K., Kukull,W.A., Kunkle,B.W., Kuwano,R.,
            Larson,E.B., Logue,M.W., Manly,J.J., Martin,E.R., Montine,T.J.,
            Mukherjee,S., Naj,A., Reiman,E.M., Reitz,C., Sherva,R., St
            George-Hyslop,P.H., Thornton,T., Younkin,S.G., Vardarajan,B.N.,
            Wang,L.S., Wendlund,J.R., Winslow,A.R., Haines,J., Mayeux,R.,
            Pericak-Vance,M.A., Schellenberg,G., Lunetta,K.L. and Farrer,L.A.
  CONSRTM   Alzheimer's Disease Genetics Consortium
  TITLE     Transethnic genome-wide scan identifies novel Alzheimer's disease
            loci
  JOURNAL   Alzheimers Dement 13 (7), 727-738 (2017)
   PUBMED   28183528
  REMARK    GeneRIF: We obtained GWS evidence (P < 2.7 x 10(-6)) for gene-based
            association in the total sample with a novel locus, TPBG (P = 1.8 x
            10(-6)).
REFERENCE   5  (bases 1 to 2890)
  AUTHORS   Kerk,S.A., Finkel,K.A., Pearson,A.T., Warner,K.A., Zhang,Z.,
            Nor,F., Wagner,V.P., Vargas,P.A., Wicha,M.S., Hurt,E.M.,
            Hollingsworth,R.E., Tice,D.A. and Nor,J.E.
  TITLE     5T4-Targeted Therapy Ablates Cancer Stem Cells and Prevents
            Recurrence of Head and Neck Squamous Cell Carcinoma
  JOURNAL   Clin Cancer Res 23 (10), 2516-2527 (2017)
   PUBMED   27780858
  REMARK    GeneRIF: Collectively, these findings demonstrate that an anti-5T4
            antibody-drug conjugate reduces the fraction of cancer stem cells
            (CSC), and prevents local recurrence and suggest a novel
            therapeutic approach for patients with head and neck squamous cell
            carcinoma.
REFERENCE   6  (bases 1 to 2890)
  AUTHORS   Awan,A., Lucic,M.R., Shaw,D.M., Sheppard,F., Westwater,C.,
            Lyons,S.A. and Stern,P.L.
  TITLE     5T4 interacts with TIP-2/GIPC, a PDZ protein, with implications for
            metastasis
  JOURNAL   Biochem Biophys Res Commun 290 (3), 1030-1036 (2002)
   PUBMED   11798178
REFERENCE   7  (bases 1 to 2890)
  AUTHORS   King,K.W., Sheppard,F.C., Westwater,C., Stern,P.L. and Myers,K.A.
  TITLE     Organisation of the mouse and human 5T4 oncofoetal leucine-rich
            glycoprotein genes and expression in foetal and adult murine
            tissues
  JOURNAL   Biochim Biophys Acta 1445 (3), 257-270 (1999)
   PUBMED   10366710
REFERENCE   8  (bases 1 to 2890)
  AUTHORS   Carsberg,C.J., Myers,K.A. and Stern,P.L.
  TITLE     Metastasis-associated 5T4 antigen disrupts cell-cell contacts and
            induces cellular motility in epithelial cells
  JOURNAL   Int J Cancer 68 (1), 84-92 (1996)
   PUBMED   8895545
REFERENCE   9  (bases 1 to 2890)
  AUTHORS   Carsberg,C.J., Myers,K.A., Evans,G.S., Allen,T.D. and Stern,P.L.
  TITLE     Metastasis-associated 5T4 oncofoetal antigen is concentrated at
            microvillus projections of the plasma membrane
  JOURNAL   J Cell Sci 108 (Pt 8), 2905-2916 (1995)
   PUBMED   7593330
REFERENCE   10 (bases 1 to 2890)
  AUTHORS   Myers,K.A., Rahi-Saund,V., Davison,M.D., Young,J.A., Cheater,A.J.
            and Stern,P.L.
  TITLE     Isolation of a cDNA encoding 5T4 oncofetal trophoblast
            glycoprotein. An antigen associated with metastasis contains
            leucine-rich repeats
  JOURNAL   J Biol Chem 269 (12), 9319-9324 (1994)
   PUBMED   8132670
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL121977.11.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: This gene encodes a leucine-rich transmembrane
            glycoprotein that may be involved in cell adhesion. The encoded
            protein is an oncofetal antigen that is specific to trophoblast
            cells. In adults this protein is highly expressed in many tumor
            cells and is associated with poor clinical outcome in numerous
            cancers. Alternate splicing in the 5' UTR results in multiple
            transcript variants that encode the same protein. [provided by
            RefSeq, Oct 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC037161.2, AK074786.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369750.4/ ENSP00000358765.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-92                AL121977.11        109825-109916
            93-2890             AL121977.11        110631-113428
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q14.1"
     gene            1..2890
                     /gene="TPBG"
                     /gene_synonym="5T4; 5T4AG; M6P1; WAIF1"
                     /note="trophoblast glycoprotein"
                     /db_xref="GeneID:7162"
                     /db_xref="HGNC:HGNC:12004"
                     /db_xref="MIM:190920"
     exon            1..92
                     /gene="TPBG"
                     /gene_synonym="5T4; 5T4AG; M6P1; WAIF1"
                     /inference="alignment:Splign:2.1.0"
     exon            93..2890
                     /gene="TPBG"
                     /gene_synonym="5T4; 5T4AG; M6P1; WAIF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             432..1694
                     /gene="TPBG"
                     /gene_synonym="5T4; 5T4AG; M6P1; WAIF1"
                     /note="5T4 oncofetal trophoblast glycoprotein; 5T4
                     oncotrophoblast glycoprotein; 5T4 oncofetal antigen;
                     wnt-activated inhibitory factor 1"
                     /codon_start=1
                     /product="trophoblast glycoprotein precursor"
                     /protein_id="NP_001363851.1"
                     /db_xref="CCDS:CCDS4995.1"
                     /db_xref="GeneID:7162"
                     /db_xref="HGNC:HGNC:12004"
                     /db_xref="MIM:190920"
                     /translation="MPGGCSRGPAAGDGRLRLARLALVLLGWVSSSSPTSSASSFSSS
                     APFLASAVSAQPPLPDQCPALCECSEAARTVKCVNRNLTEVPTDLPAYVRNLFLTGNQ
                     LAVLPAGAFARRPPLAELAALNLSGSRLDEVRAGAFEHLPSLRQLDLSHNPLADLSPF
                     AFSGSNASVSAPSPLVELILNHIVPPEDERQNRSFEGMVVAALLAGRALQGLRRLELA
                     SNHFLYLPRDVLAQLPSLRHLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNG
                     TLAELQGLPHIRVFLDNNPWVCDCHMADMVTWLKETEVVQGKDRLTCAYPEKMRNRVL
                     LELNSADLDCDPILPPSLQTSYVFLGIVLALIGAIFLLVLYLNRKGIKKWMHNIRDAC
                     RDHMEGYHYRYEINADPRLTNLSSNSDV"
     regulatory      2380..2385
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPBG"
                     /gene_synonym="5T4; 5T4AG; M6P1; WAIF1"
                     /note="hexamer: AATAAA"
     polyA_site      2399
                     /gene="TPBG"
                     /gene_synonym="5T4; 5T4AG; M6P1; WAIF1"
                     /note="major polyA site"
     regulatory      2861..2866
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPBG"
                     /gene_synonym="5T4; 5T4AG; M6P1; WAIF1"
                     /note="hexamer: AATAAA"
     regulatory      2870..2875
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TPBG"
                     /gene_synonym="5T4; 5T4AG; M6P1; WAIF1"
                     /note="hexamer: TATAAA"
     polyA_site      2881
                     /gene="TPBG"
                     /gene_synonym="5T4; 5T4AG; M6P1; WAIF1"
     polyA_site      2890
                     /gene="TPBG"
                     /gene_synonym="5T4; 5T4AG; M6P1; WAIF1"
                     /note="major polyA site"
ORIGIN      
        1 gcgaagagag tcggcgcccg caacgcggag ccgggaagtc gtcgctactc tggtggaact
       61 cagagttggt tctggaggcg gcggacgcgg aggtccctca gaggatccag cgaggggcgc
      121 caacaagagg cgaagaggtg gcaccagggc ggcggcagga agaggagcgg gagcaggagc
      181 gcggagcgga gcgtcccgac ccgccgtgcg tactttctgg agggaagggg cgggggaatc
      241 ggcccctgag ggaagcgccc ggtggcgagg gggttagcca agttccggct gcggcgccac
      301 tccctcggtt ccacgagagg aaagtttttt ttttccagac gcttccgccg gctcgcgccc
      361 tccgggccca gcctcccgag ccttcggagc gggcgccgtc ccagcccagc tccggggaaa
      421 cgcgagccgc gatgcctggg gggtgctccc ggggccccgc cgccggggac gggcgtctgc
      481 ggctggcgcg actagcgctg gtactcctgg gctgggtctc ctcgtcttct cccacctcct
      541 cggcatcctc cttctcctcc tcggcgccgt tcctggcttc cgccgtgtcc gcccagcccc
      601 cgctgccgga ccagtgcccc gcgctgtgcg agtgctccga ggcagcgcgc acagtcaagt
      661 gcgttaaccg caatctgacc gaggtgccca cggacctgcc cgcctacgtg cgcaacctct
      721 tccttaccgg caaccagctg gccgtgctcc ctgccggcgc cttcgcccgc cggccgccgc
      781 tggcggagct ggccgcgctc aacctcagcg gcagccgcct ggacgaggtg cgcgcgggcg
      841 ccttcgagca tctgcccagc ctgcgccagc tcgacctcag ccacaaccca ctggccgacc
      901 tcagtccctt cgctttctcg ggcagcaatg ccagcgtctc ggcccccagt ccccttgtgg
      961 aactgatcct gaaccacatc gtgccccctg aagatgagcg gcagaaccgg agcttcgagg
     1021 gcatggtggt ggcggccctg ctggcgggcc gtgcactgca ggggctccgc cgcttggagc
     1081 tggccagcaa ccacttcctt tacctgccgc gggatgtgct ggcccaactg cccagcctca
     1141 ggcacctgga cttaagtaat aattcgctgg tgagcctgac ctacgtgtcc ttccgcaacc
     1201 tgacacatct agaaagcctc cacctggagg acaatgccct caaggtcctt cacaatggca
     1261 ccctggctga gttgcaaggt ctaccccaca ttagggtttt cctggacaac aatccctggg
     1321 tctgcgactg ccacatggca gacatggtga cctggctcaa ggaaacagag gtagtgcagg
     1381 gcaaagaccg gctcacctgt gcatatccgg aaaaaatgag gaatcgggtc ctcttggaac
     1441 tcaacagtgc tgacctggac tgtgacccga ttcttccccc atccctgcaa acctcttatg
     1501 tcttcctggg tattgtttta gccctgatag gcgctatttt cctcctggtt ttgtatttga
     1561 accgcaaggg gataaaaaag tggatgcata acatcagaga tgcctgcagg gatcacatgg
     1621 aagggtatca ttacagatat gaaatcaatg cggaccccag attaacgaac ctcagttcta
     1681 actcggatgt ctgagaaata ttagaggaca gaccaaggac aactctgcat gagatgtaga
     1741 cttaagcttt atccctacta ggcttgctcc actttcatcc tccactatag atacaacgga
     1801 ctttgactaa aagcagtgaa ggggatttgc ttccttgtta tgtaaagttt ctcggtgtgt
     1861 tctgttaatg taagacgatg aacagttgtg tatagtgttt taccctcttc tttttcttgg
     1921 aactcctcaa cacgtatgga gggatttttc aggtttcagc atgaacatgg gcttcttgct
     1981 gtctgtctct ctctcagtac agttcaaggt gtagcaagtg tacccacaca gatagcattc
     2041 aacaaaagct gcctcaactt tttcgagaaa aatactttat tcataaatat cagttttatt
     2101 ctcatgtacc taagttgtgg agaaaataat tgcatcctat aaactgcctg cagacgttag
     2161 caggctcttc aaaataactc catggtgcac aggagcacct gcatccaaga gcatgcttac
     2221 attttactgt tctgcatatt acaaaaaata acttgcaact tcataacttc tttgacaaag
     2281 taaattactt ttttgattgc agtttatatg aaaatgtact gatttttttt taataaactg
     2341 catcgagatc caaccgactg aattgttaaa aaaaaaaaaa ataaagattc ttaaaagaat
     2401 tacagtgtgt gcaagtttgc tttgaaaaaa ggatgaaggg caggagtatt caatggtctt
     2461 ggttccgatg ataattatta cttaacatag ctgagaatca aactgtagca atgtctaata
     2521 taaatagact tgggagatcg ttttgaaatg gtggatcctt ttagtattta aatggagagt
     2581 ttgagtattc atgtgactgt atggtcccaa agttaatgct atttatgaaa cagttttgta
     2641 agaacataat gaagttggta aaagctaagc gttctgcaca ataaacagtt cccagtttgg
     2701 ggctttttca aggctaatga agttattgtt ggtctagaaa aatagtattt acttaataaa
     2761 actctgtttg aaattgttct gtggattttt agagcagaat ttgaaacaaa tgtaatgcaa
     2821 ataaatataa agataaagtg tcttaaagac aatattctga aataaaagat ataaaatata
     2881 atttaaaaaa
//
