LOCUS       NM_001031685            4632 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens tumor protein p53 binding protein 2 (TP53BP2),
            transcript variant 1, mRNA.
ACCESSION   NM_001031685
VERSION     NM_001031685.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Guan,G., Zhang,T., Ning,J., Tao,C., Gao,N., Zeng,Z., Guo,H.,
            Chen,C.C., Yang,J., Zhang,J., Gu,W., Yang,E., Liu,R., Guo,X.,
            Ren,S., Wang,L., Wei,G., Zheng,S., Gao,Z., Chen,X., Lu,F. and
            Chen,X.
  TITLE     Higher TP53BP2 expression is associated with HBsAg loss in
            peginterferon-alpha-treated patients with chronic hepatitis B
  JOURNAL   J Hepatol 80 (1), 41-52 (2024)
   PUBMED   37858684
  REMARK    GeneRIF: Higher TP53BP2 expression is associated with HBsAg loss in
            peginterferon-alpha-treated patients with chronic hepatitis B.
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Smirnov,A., Magri,A., Lotz,R., Han,X., Yin,C., Harris,M.,
            Osterburg,C., Dotsch,V., McKeating,J.A. and Lu,X.
  TITLE     ASPP2 binds to hepatitis C virus NS5A protein via an SH3
            domain/PxxP motif-mediated interaction and potentiates infection
  JOURNAL   J Gen Virol 104 (9) (2023)
   PUBMED   37750869
  REMARK    GeneRIF: ASPP2 binds to hepatitis C virus NS5A protein via an SH3
            domain/PxxP motif-mediated interaction and potentiates infection.
REFERENCE   3  (bases 1 to 4632)
  AUTHORS   Wang,Y., Yan,Y., Huo,Y., Pang,Y., Chan,L., Wang,S., Chen,D., Lin,D.
            and Wang,W.
  TITLE     mRNA sequencing and CyTOF analysis revealed ASPP2 altered the
            response patterns of hepatocellular carcinoma HepG2 cells to usnic
            acid
  JOURNAL   Naunyn Schmiedebergs Arch Pharmacol 396 (8), 1847-1856 (2023)
   PUBMED   36877271
  REMARK    GeneRIF: mRNA sequencing and CyTOF analysis revealed ASPP2 altered
            the response patterns of hepatocellular carcinoma HepG2 cells to
            usnic acid.
REFERENCE   4  (bases 1 to 4632)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   5  (bases 1 to 4632)
  AUTHORS   Takahashi,N., Kobayashi,S., Jiang,X., Kitagori,K., Imai,K., Hibi,Y.
            and Okamoto,T.
  TITLE     Expression of 53BP2 and ASPP2 proteins from TP53BP2 gene by
            alternative splicing
  JOURNAL   Biochem Biophys Res Commun 315 (2), 434-438 (2004)
   PUBMED   14766226
  REMARK    GeneRIF: TP53BP2 encodes two mRNA species, either with (53BP2) or
            without exon 3 (ASPP2), by alternative splicing in various cell
            lines and tissues
REFERENCE   6  (bases 1 to 4632)
  AUTHORS   Pirozzi,G., McConnell,S.J., Uveges,A.J., Carter,J.M., Sparks,A.B.,
            Kay,B.K. and Fowlkes,D.M.
  TITLE     Identification of novel human WW domain-containing proteins by
            cloning of ligand targets
  JOURNAL   J Biol Chem 272 (23), 14611-14616 (1997)
   PUBMED   9169421
REFERENCE   7  (bases 1 to 4632)
  AUTHORS   Gorina,S. and Pavletich,N.P.
  TITLE     Structure of the p53 tumor suppressor bound to the ankyrin and SH3
            domains of 53BP2
  JOURNAL   Science 274 (5289), 1001-1005 (1996)
   PUBMED   8875926
REFERENCE   8  (bases 1 to 4632)
  AUTHORS   Naumovski,L. and Cleary,M.L.
  TITLE     The p53-binding protein 53BP2 also interacts with Bc12 and impedes
            cell cycle progression at G2/M
  JOURNAL   Mol Cell Biol 16 (7), 3884-3892 (1996)
   PUBMED   8668206
REFERENCE   9  (bases 1 to 4632)
  AUTHORS   Helps,N.R., Barker,H.M., Elledge,S.J. and Cohen,P.T.
  TITLE     Protein phosphatase 1 interacts with p53BP2, a protein which binds
            to the tumour suppressor p53
  JOURNAL   FEBS Lett 377 (3), 295-300 (1995)
   PUBMED   8549741
REFERENCE   10 (bases 1 to 4632)
  AUTHORS   Iwabuchi,K., Bartel,P.L., Li,B., Marraccino,R. and Fields,S.
  TITLE     Two cellular proteins that bind to wild-type but not mutant p53
  JOURNAL   Proc Natl Acad Sci U S A 91 (13), 6098-6102 (1994)
   PUBMED   8016121
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX425756.2, BC058918.1,
            AA961496.1 and AI042183.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001031685.2.
            
            Summary: This gene encodes a member of the ASPP
            (apoptosis-stimulating protein of p53) family of p53 interacting
            proteins. The protein contains four ankyrin repeats and an SH3
            domain involved in protein-protein interactions. It is localized to
            the perinuclear region of the cytoplasm, and regulates apoptosis
            and cell growth through interactions with other regulatory
            molecules including members of the p53 family. Multiple transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript but encodes the longer isoform (1), also known as 53BP2L
            or long.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC058918.1, AJ318888.2 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000343537.12/ ENSP00000341957.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                BX425756.2         26-42
            18-4437             BC058918.1         1-4420
            4438-4631           AA961496.1         1-194               c
            4632-4632           AI042183.1         14-14               c
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q41"
     gene            1..4632
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /note="tumor protein p53 binding protein 2"
                     /db_xref="GeneID:7159"
                     /db_xref="HGNC:HGNC:12000"
                     /db_xref="MIM:602143"
     exon            1..294
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /inference="alignment:Splign:2.1.0"
     CDS             268..3672
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /note="isoform 1 is encoded by transcript variant 1;
                     BCL2-binding protein; tumor suppressor p53-binding protein
                     2; apoptosis-stimulating protein of p53, 2; renal
                     carcinoma antigen NY-REN-51"
                     /codon_start=1
                     /product="apoptosis-stimulating of p53 protein 2 isoform
                     1"
                     /protein_id="NP_001026855.2"
                     /db_xref="CCDS:CCDS44319.1"
                     /db_xref="GeneID:7159"
                     /db_xref="HGNC:HGNC:12000"
                     /db_xref="MIM:602143"
                     /translation="MRFGSKMMPMFLTVYLSNNEQHFTEVPVTPETICRDVVDLCKEP
                     GESDCHLAEVWCGSERPVADNERMFDVLQRFGSQRNEVRFFLRHERPPGRDIVSGPRS
                     QDPSLKRNGVKVPGEYRRKENGVNSPRMDLTLAELQEMASRQQQQIEAQQQLLATKEQ
                     RLKFLKQQDQRQQQQVAEQEKLKRLKEIAENQEAKLKKVRALKGHVEQKRLSNGKLVE
                     EIEQMNNLFQQKQRELVLAVSKVEELTRQLEMLKNGRIDSHHDNQSAVAELDRLYKEL
                     QLRNKLNQEQNAKLQQQRECLNKRNSEVAVMDKRVNELRDRLWKKKAALQQKENLPVS
                     SDGNLPQQAASAPSRVAAVGPYIQSSTMPRMPSRPELLVKPALPDGSLVIQASEGPMK
                     IQTLPNMRSGAASQTKGSKIHPVGPDWSPSNADLFPSQGSASVPQSTGNALDQVDDGE
                     VPLREKEKKVRPFSMFDAVDQSNAPPSFGTLRKNQSSEDILRDAQVANKNVAKVPPPV
                     PTKPKQINLPYFGQTNQPPSDIKPDGSSQQLSTVVPSMGTKPKPAGQQPRVLLSPSIP
                     SVGQDQTLSPGSKQESPPAAAVRPFTPQPSKDTLLPPFRKPQTVAASSIYSMYTQQQA
                     PGKNFQQAVQSALTKTHTRGPHFSSVYGKPVIAAAQNQQQHPENIYSNSQGKPGSPEP
                     ETEPVSSVQENHENERIPRPLSPTKLLPFLSNPYRNQSDADLEALRKKLSNAPRPLKK
                     RSSITEPEGPNGPNIQKLLYQRTTIAAMETISVPSYPSKSASVTASSESPVEIQNPYL
                     HVEPEKEVVSLVPESLSPEDVGNASTENSDMPAPSPGLDYEPEGVPDNSPNLQNNPEE
                     PNPEAPHVLDVYLEEYPPYPPPPYPSGEPEGPGEDSVSMRPPEITGQVSLPPGKRTNL
                     RKTGSERIAHGMRVKFNPLALLLDSSLEGEFDLVQRIIYEVDDPSLPNDEGITALHNA
                     VCAGHTEIVKFLVQFGVNVNAADSDGWTPLHCAASCNNVQVCKFLVESGAAVFAMTYS
                     DMQTAADKCEEMEEGYTQCSQFLYGVQEKMGIMNKGVIYALWDYEPQNDDELPMKEGD
                     CMTIIHREDEDEIEWWWARLNDKEGYVPRNLLGLYPRIKPRQRSLA"
     exon            295..442
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /inference="alignment:Splign:2.1.0"
     exon            443..556
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /inference="alignment:Splign:2.1.0"
     exon            557..639
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /inference="alignment:Splign:2.1.0"
     exon            640..741
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /inference="alignment:Splign:2.1.0"
     exon            742..916
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1098
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1263
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /inference="alignment:Splign:2.1.0"
     exon            1264..1492
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /inference="alignment:Splign:2.1.0"
     exon            1493..1603
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..1752
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..2215
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /inference="alignment:Splign:2.1.0"
     exon            2216..2991
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /inference="alignment:Splign:2.1.0"
     exon            2992..3129
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /inference="alignment:Splign:2.1.0"
     exon            3130..3263
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /inference="alignment:Splign:2.1.0"
     exon            3264..3430
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /inference="alignment:Splign:2.1.0"
     exon            3431..3630
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /inference="alignment:Splign:2.1.0"
     exon            3631..4632
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4419..4424
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /note="hexamer: AATAAA"
     polyA_site      4437
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /note="major polyA site"
     regulatory      4606..4611
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
                     /note="hexamer: AATAAA"
     polyA_site      4632
                     /gene="TP53BP2"
                     /gene_synonym="53BP2; ASPP2; BBP; P53BP2; PPP1R13A"
ORIGIN      
        1 ggtttcggag cggaggaggg agccccgacc gtcacgagcg tcgaagagac aaagccgcgt
       61 cagggggccc ggccggggcg ggggagcccg gggcttgttg gtgccccagc ccgcgcggag
      121 ggcccttcgg acccgcgcgc cgccgctgcc gccgccgccg cctcgcaaca ggtccgggcg
      181 gcctcgctct ccgctcccct cccccgcatc cgcgaccctc cggggcacct cagctcggcc
      241 ggggccgcag tctggccacc cgcttccatg cggttcgggt ccaagatgat gccgatgttt
      301 cttaccgtgt atctcagtaa caatgagcag cacttcacag aagttccagt tactccagaa
      361 acaatatgca gagacgtggt ggatctgtgc aaagaacccg gcgagagtga ttgccatttg
      421 gctgaagtgt ggtgtggctc tgaacgtcca gttgcggata atgagcgaat gtttgatgtt
      481 cttcaacgat ttggaagtca gaggaacgaa gttcgcttct tccttcgtca tgaacgcccc
      541 cctggcaggg acattgtgag tggaccaaga tctcaggatc caagtttaaa aagaaatggt
      601 gtaaaagttc ctggtgaata tcgaagaaag gagaacggtg ttaatagtcc taggatggat
      661 ctgactcttg ctgaacttca ggaaatggca tctcgccagc agcaacagat tgaagcccag
      721 caacaattgc tggcaactaa ggaacagcgc ttaaagtttt tgaaacaaca agatcagcga
      781 caacagcaac aagttgctga gcaggagaaa cttaaaaggc taaaagaaat agctgagaat
      841 caggaagcta agctaaaaaa agtgagagca cttaaaggcc acgtggaaca gaagagacta
      901 agcaatggga aacttgtgga ggaaattgaa cagatgaata atttgttcca gcaaaaacag
      961 agggagctcg tcctggctgt gtcaaaagta gaagaactga ccaggcagct agagatgctc
     1021 aagaacggca ggatcgacag ccaccatgac aatcagtctg cagtggctga gcttgatcgc
     1081 ctctataagg agctgcagct aagaaacaaa ttgaatcaag agcagaatgc caagctacaa
     1141 caacagaggg agtgtttgaa taagcgtaat tcagaagtgg cagtcatgga taagcgtgtt
     1201 aatgagctga gggaccggct gtggaagaag aaggcagctc tacagcaaaa agaaaatcta
     1261 ccagtttcat ctgatggaaa tcttccccag caagccgcgt cagccccaag ccgtgtggct
     1321 gcagtaggtc cctatatcca gtcgtctact atgcctcgga tgccctcaag gcctgaattg
     1381 ctggtgaagc cagccctgcc ggatggttcc ttggtcattc aggcttcaga ggggccgatg
     1441 aaaatacaga cactgcccaa catgagatct ggggctgctt cacaaactaa aggctctaaa
     1501 atccatccag ttggccctga ttggagtcct tcaaatgcag atcttttccc aagccaaggc
     1561 tctgcttctg tacctcaaag cactgggaat gctctggatc aagttgatga tggagaggtt
     1621 ccgctgaggg agaaagagaa gaaagtgcgt ccgttctcaa tgtttgatgc agtagaccag
     1681 tccaatgccc caccttcctt tggtactctg aggaagaacc agagcagtga agatatcttg
     1741 cgggatgctc aggttgcaaa taaaaatgtg gctaaagtac cacctcctgt tcctacaaaa
     1801 ccaaaacaga ttaatttgcc ttattttgga caaactaatc agccaccttc agacattaag
     1861 ccagacggaa gttctcagca gttgtcaaca gttgttccgt ccatgggaac taaaccaaaa
     1921 ccagcagggc agcagccgag agtgctgcta tctcccagca taccttcggt tggccaagac
     1981 cagacccttt ctccaggttc taagcaagaa agtccacctg ctgctgccgt ccggcccttt
     2041 actccccagc cttccaaaga caccttactt ccacccttca gaaaacccca gaccgtggca
     2101 gcaagttcaa tatattccat gtatacgcaa cagcaggcgc caggaaaaaa cttccagcag
     2161 gctgtgcaga gcgcgttgac caagactcat accagagggc cacacttttc aagtgtatat
     2221 ggtaagcctg taattgctgc tgcccagaat caacagcagc acccagagaa catttattcc
     2281 aatagccagg gcaagcctgg cagtccagaa cctgaaacag agcctgtttc ttcagttcag
     2341 gagaaccatg aaaacgaaag aattcctcgg ccactcagcc caactaaatt actgcctttc
     2401 ttatctaatc cttaccgaaa ccagagtgat gctgacctag aagccttacg aaagaaactg
     2461 tctaacgcac caaggcctct aaagaaacgt agttctatta cagagccaga gggtcctaat
     2521 gggccaaata ttcagaagct tttatatcag aggaccacca tagcggccat ggagaccatc
     2581 tctgtcccat catacccatc caagtcagct tctgtgactg ccagctcaga aagcccagta
     2641 gaaatccaga atccatattt acatgtggag cccgaaaagg aggtggtctc tctggttcct
     2701 gaatcattgt ccccagagga tgtggggaat gccagtacag agaacagtga catgccagct
     2761 ccttctccag gccttgatta tgagcctgag ggagtcccag acaacagccc aaatctccag
     2821 aataacccag aagaaccaaa tccagaggct ccacatgtgc ttgatgtgta cctggaggag
     2881 taccctccat acccaccccc accataccca tctggggagc ctgaagggcc cggagaagac
     2941 tcggtgagca tgcgcccgcc tgaaatcacc gggcaggtct ctctgcctcc tggtaaaagg
     3001 acaaacttgc gtaaaactgg ctcagagcgt atcgctcatg gaatgagggt gaaattcaac
     3061 ccccttgctt tactgctaga ttcgtctttg gagggagaat ttgaccttgt acagagaatt
     3121 atttatgagg ttgatgaccc aagcctcccc aatgatgaag gcatcacggc tcttcacaat
     3181 gctgtgtgtg caggccacac agaaatcgtt aagttcctgg tacagtttgg tgtaaatgta
     3241 aatgctgctg atagtgatgg atggactcca ttacattgtg ctgcctcatg taacaacgtc
     3301 caagtgtgta agtttttggt ggagtcagga gccgctgtgt ttgccatgac ctacagtgac
     3361 atgcagactg ctgcagataa gtgcgaggaa atggaggaag gctacactca gtgctcccaa
     3421 tttctttatg gagttcagga gaagatgggc ataatgaata aaggagtcat ttatgcgctt
     3481 tgggattatg aacctcagaa tgatgatgag ctgcccatga aagaaggaga ctgcatgaca
     3541 atcatccaca gggaagacga agatgaaatc gaatggtggt gggcgcgcct taatgataag
     3601 gagggatatg ttccacgtaa cttgctggga ctgtacccaa gaattaaacc aagacaaagg
     3661 agcttggcct gaaacttcca cacagaattt tagtcaatga agaattaatc tctgttaaga
     3721 agaagtaata cgattatttt tggcaaaaat ttcacaagac ttattttaat gacaatgtag
     3781 cttgaaagcg atgaagaatg tctctagaag agaatgaagg attgaagaat tcaccattag
     3841 aggacattta gcgtgatgaa ataaagcatc tacgtcagca ggccatactg tgttggggca
     3901 aaggtgtccc gtgtagcact cagataagta tacagcgaca atcctgtttt ctacaagaat
     3961 cctgtctagt aaataggatc atttattggg cagttgggaa atcagctctc tgtcctgttg
     4021 agtgttttca gcagctgctc ctaaaccagt cctcctgcca gaaaggacca gtgccgtcac
     4081 atcgctgtct ctgattgtcc ccggcaccag caggcccttg gggggctcac ctgaaggctc
     4141 gaaggcactg cacacttgta tattgtcagt gaagaactgt tagttggttg tcagtgaaca
     4201 ataactttat tatatgagtt tttgtagcat cttaagaatt atacatatgt ttgaaatatt
     4261 gaaactaagc tacggtacca gtaattagat gtagaatctt gtttgtaggc tgaattttaa
     4321 tctgtattta ttgtcttttg tatctcagaa attagaaact tgctacagac ttacccgtaa
     4381 tatttgtcaa gatcatagct gactttaaaa acagttgtaa taaacttttt gatgctagct
     4441 gtttactggt ttttgttttt gatgtcataa atagaccttg tttaatagtc acaagccgtt
     4501 gggatatcat acccagctga aaaagaacaa actgcttaac ataagtatat gtatcgtaat
     4561 aagagttttt taccagctaa gtgattcaat gtaagtggtt tttaaaataa aatactgtag
     4621 agatcatggt ga
//
