LOCUS       NM_001411050           10591 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens tumor protein p53 binding protein 1 (TP53BP1),
            transcript variant 5, mRNA.
ACCESSION   NM_001411050 XM_047432996
VERSION     NM_001411050.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Lim,B., Matsui,Y., Jung,S., Djekidel,M.N., Qi,W., Yuan,Z.F.,
            Wang,X., Yang,X., Connolly,N., Pilehroud,A.S., Pan,H., Wang,F.,
            Pruett-Miller,S.M., Kavdia,K., Pagala,V., Fan,Y., Peng,J., Xu,B.
            and Peng,J.C.
  TITLE     Phosphorylation of the DNA damage repair factor 53BP1 by ATM kinase
            controls neurodevelopmental programs in cortical brain organoids
  JOURNAL   PLoS Biol 22 (9), e3002760 (2024)
   PUBMED   39226322
  REMARK    GeneRIF: Phosphorylation of the DNA damage repair factor 53BP1 by
            ATM kinase controls neurodevelopmental programs in cortical brain
            organoids.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10591)
  AUTHORS   Sun,Y., Patterson-Fortin,J., Han,S., Li,Z., Nowicka,Z.,
            Hirohashi,Y., Kilgas,S., Yi,J.K., Spektor,A., Fendler,W.,
            Konstantinopoulos,P.A. and Chowdhury,D.
  TITLE     53BP1 loss elicits cGAS-STING-dependent antitumor immunity in
            ovarian and pancreatic cancer
  JOURNAL   Nat Commun 15 (1), 6676 (2024)
   PUBMED   39107288
  REMARK    GeneRIF: 53BP1 loss elicits cGAS-STING-dependent antitumor immunity
            in ovarian and pancreatic cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10591)
  AUTHORS   Kelliher,J.L., Folkerts,M.L., Shen,K.V., Song,W., Tengler,K.,
            Stiefel,C.M., Lee,S.O., Dray,E., Zhao,W., Koss,B., Pannunzio,N.R.
            and Leung,J.W.
  TITLE     Evolved histone tail regulates 53BP1 recruitment at damaged
            chromatin
  JOURNAL   Nat Commun 15 (1), 4634 (2024)
   PUBMED   38821984
  REMARK    GeneRIF: Evolved histone tail regulates 53BP1 recruitment at
            damaged chromatin.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10591)
  AUTHORS   Panier,S. and Boulton,S.J.
  TITLE     Double-strand break repair: 53BP1 comes into focus
  JOURNAL   Nat Rev Mol Cell Biol 15 (1), 7-18 (2014)
   PUBMED   24326623
  REMARK    GeneRIF: 53BP1 recruitment to DNA repair foci requires the direct
            recognition of a double strand break-specific histone code.
            [Review]
            Review article
REFERENCE   5  (bases 1 to 10591)
  AUTHORS   Dimitrova,N., Chen,Y.C., Spector,D.L. and de Lange,T.
  TITLE     53BP1 promotes non-homologous end joining of telomeres by
            increasing chromatin mobility
  JOURNAL   Nature 456 (7221), 524-528 (2008)
   PUBMED   18931659
  REMARK    GeneRIF: 53BP1 (also known as TRP53BP1), a component of DNA damage
            foci, changes the dynamic behaviour of chromatin to promote DNA
            repair
REFERENCE   6  (bases 1 to 10591)
  AUTHORS   DiTullio,R.A. Jr., Mochan,T.A., Venere,M., Bartkova,J.,
            Sehested,M., Bartek,J. and Halazonetis,T.D.
  TITLE     53BP1 functions in an ATM-dependent checkpoint pathway that is
            constitutively activated in human cancer
  JOURNAL   Nat Cell Biol 4 (12), 998-1002 (2002)
   PUBMED   12447382
  REMARK    GeneRIF: 53BP1 functions as a DNA damage checkpoint protein
            Erratum:[Nat Cell Biol. 2003 Jan;5(1):84.]
REFERENCE   7  (bases 1 to 10591)
  AUTHORS   Wang,B., Matsuoka,S., Carpenter,P.B. and Elledge,S.J.
  TITLE     53BP1, a mediator of the DNA damage checkpoint
  JOURNAL   Science 298 (5597), 1435-1438 (2002)
   PUBMED   12364621
  REMARK    GeneRIF: demonstrated that 53BP1 is a key transducer of the DNA
            damage checkpoint signal; required for p53 accumulation, G2-M
            checkpoint arrest, and the intra-S-phase checkpoint in response to
            ionizing radiation
REFERENCE   8  (bases 1 to 10591)
  AUTHORS   Choubey,D., Li,S.J., Datta,B., Gutterman,J.U. and Lengyel,P.
  TITLE     Inhibition of E2F-mediated transcription by p202
  JOURNAL   EMBO J 15 (20), 5668-5678 (1996)
   PUBMED   8896460
REFERENCE   9  (bases 1 to 10591)
  AUTHORS   Choubey,D. and Lengyel,P.
  TITLE     Binding of an interferon-inducible protein (p202) to the
            retinoblastoma protein
  JOURNAL   J Biol Chem 270 (11), 6134-6140 (1995)
   PUBMED   7890747
REFERENCE   10 (bases 1 to 10591)
  AUTHORS   Iwabuchi,K., Bartel,P.L., Li,B., Marraccino,R. and Fields,S.
  TITLE     Two cellular proteins that bind to wild-type but not mutant p53
  JOURNAL   Proc Natl Acad Sci U S A 91 (13), 6098-6102 (1994)
   PUBMED   8016121
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC018924.9.
            
            On Aug 20, 2022 this sequence version replaced XM_047432996.1.
            
            Summary: This gene encodes a protein that functions in the DNA
            double-strand break repair pathway choice, promoting non-homologous
            end joining (NHEJ) pathways, and limiting homologous recombination.
            This protein plays multiple roles in the DNA damage response,
            including promoting checkpoint signaling following DNA damage,
            acting as a scaffold for recruitment of DNA damage response
            proteins to damaged chromatin, and promoting NHEJ pathways by
            limiting end resection following a double-strand break. These roles
            are also important during V(D)J recombination, class switch
            recombination and at unprotected telomeres. Alternative splicing
            results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Aug 2017].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038192.3772763.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-271               AC018924.9         180703-180973       c
            272-492             AC018924.9         163370-163590       c
            493-677             AC018924.9         162617-162801       c
            678-771             AC018924.9         162335-162428       c
            772-856             AC018924.9         162002-162086       c
            857-984             AC018924.9         151228-151355       c
            985-1143            AC018924.9         150192-150350       c
            1144-1273           AC018924.9         149730-149859       c
            1274-1440           AC018924.9         147926-148092       c
            1441-1570           AC018924.9         145898-146027       c
            1571-1665           AC018924.9         145006-145100       c
            1666-1874           AC018924.9         140191-140399       c
            1875-3201           AC018924.9         126225-127551       c
            3202-3321           AC018924.9         117699-117818       c
            3322-3525           AC018924.9         116720-116923       c
            3526-3583           AC018924.9         111859-111916       c
            3584-3676           AC018924.9         108657-108749       c
            3677-4160           AC018924.9         102527-103010       c
            4161-4313           AC018924.9         98349-98501         c
            4314-4585           AC018924.9         92188-92459         c
            4586-5016           AC018924.9         90638-91068         c
            5017-5208           AC018924.9         86558-86749         c
            5209-5424           AC018924.9         85927-86142         c
            5425-5640           AC018924.9         83452-83667         c
            5641-5735           AC018924.9         79980-80074         c
            5736-5935           AC018924.9         79230-79429         c
            5936-6081           AC018924.9         78276-78421         c
            6082-10591          AC018924.9         73394-77903         c
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.3"
     gene            1..10591
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /note="tumor protein p53 binding protein 1"
                     /db_xref="GeneID:7158"
                     /db_xref="HGNC:HGNC:11999"
                     /db_xref="MIM:605230"
     exon            1..271
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            272..492
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     CDS             486..6269
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /note="isoform 5 is encoded by transcript variant 5; tumor
                     protein 53-binding protein, 1; tumor suppressor
                     p53-binding protein 1; TP53-binding protein 1"
                     /codon_start=1
                     /product="TP53-binding protein 1 isoform 5"
                     /protein_id="NP_001397979.1"
                     /db_xref="CCDS:CCDS91987.1"
                     /db_xref="GeneID:7158"
                     /db_xref="HGNC:HGNC:11999"
                     /db_xref="MIM:605230"
                     /translation="MPGEQMDPTGSQLDSDFSQQDTPCLIIEDSQPESQVLEDDSGSH
                     FSMLSRHLPNLQTHKENPVLDVVSNPEQTAGEERGDGNSGFNEHLKENKVADPVDSSN
                     LDTCGSISQVIEQLPQPNRTSSVLGMSVESAPAVEEEKGEELEQKEKEKEEDTSGNTT
                     HSLGAEDTASSQLGFGVLELSQSQDVEENTVPYEVDKEQLQSVTTNSGYTRLSDVDAN
                     TAIKHEEQSNEDIPIAEQSSKDIPVTAQPSKDVHVVKEQNPPPARSEDMPFSPKASVA
                     AMEAKEQLSAQELMESGLQIQKSPEPEVLSTQEDLFDQSNKTVSSDGCSTPSREEGGC
                     SLASTPATTLHLLQLSGQRSLVQDSLSTNSSDLVAPSPDAFRSTPFIVPSSPTEQEGR
                     QDKPMDTSVLSEEGGEPFQKKLQSGEPVELENPPLLPESTVSPQASTPISQSTPVFPP
                     GSLPIPSQPQFSHDIFIPSPSLEEQSNDGKKDGDMHSSSLTVECSKTSEIEPKNSPED
                     LGLSLTGDSCKLMLSTSEYSQSPKMESLSSHRIDEDGENTQIEDTEPMSPVLNSKFVP
                     AENDSILMNPAQDGEVQLSQNDDKTKGDDTDTRDDISILATGCKGREETVAEDVCIDL
                     TCDSGSQAVPSPATRSEALSSVLDQEEAMEIKEHHPEEGSSGSEVEEIPETPCESQGE
                     ELKEENMESVPLHLSLTETQSQGLCLQKEMPKKECSEAMEVETSVISIDSPQKLAILD
                     QELEHKEQEAWEEATSEDSSVVIVDVKEPSPRVDVSCEPLEGVEKCSDSQSWEDIAPE
                     IEPCAENRLDTKEEKSVEYEGDLKSGTAETEPVEQDSSQPSLPLVRADDPLRLDQELQ
                     QPQTQEKTSNSLTEDSKMANAKQLSSDAEAQKLGKPSAHASQSFCESSSETPFHFTLP
                     KEGDIIPPLTGATPPLIGHLKLEPKRHSTPIGISNYPESTIATSDVMSESMVETHDPI
                     LGSGKGDSGAAPDVDDKLCLRMKLVSPETEASEESLQFNLEKPATGERKNGSTAVAES
                     VASPQKTMSVLSCICEARQENEARSEDPPTTPIRGNLLHFPSSQGEEEKEKLEGDHTI
                     RQSQQPMKPISPVKDPVSPASQKMVIQGPSSPQGEAMVTDVLEDQKEGRSTNKENPSK
                     ALIERPSQNNIGIQTMECSLRVPETVSAATQTIKNVCEQGTSTVDQNFGKQDATVQTE
                     RGSGEKPVSAPGDDTESLHSQGEEEFDMPQPPHGHVLHRHMRTIREVRTLVTRVITDV
                     YYVDGTEVERKVTEETEEPIVECQECETEVSPSQTGGSSGDLGDISSFSSKASSLHRT
                     SSGTSLSAMHSSGSSGKGAGPLRGKTSGTEPADFALPSSRGGPGKLRETAVPGPLGIE
                     DISPNLSPDDKSFSRVVPRVPDSTRRTDVGAGALRRSDSPEIPFQAAAGPSDGLDASS
                     PGNSFVGLRVVAKWSSNGYFYSGKITRDVGAGKYKLLFDDGYECDVLGKDILLCDPIP
                     LDTEVTALSEDEYFSAGVVKGHRKESGELYYSIEKEGQRKWYKRMAVILSLEQGNRLR
                     EQYGLGPYEAVTPLTKAADISLDNLVEGKRKRRSNVSSPATPTASSSSSTTPTRKITE
                     SPRASMGVLSGKRKLITSEEERSPAKRGRKSATVKPGAVGAGEFVSPCESGDNTGEPS
                     ALEEQRGPLPLNKTLFLGYAFLLTMATTSDKLASRSKLPDGPTGSSEEEEEFLEIPPF
                     NKQYTESQLRAGAGYILEDFNEAQCNTAYQCLLIADQHCRTRKYFLCLASGIPCVSHV
                     WVHDSCHANQLQNYRNYLLPAGYSLEEQRILDWQPRENPFQNLKVLLVSDQQQNFLEL
                     WSEILMTGGAASVKQHHSSAHNKDIALGVFDVVVTDPSCPASVLKCAEALQLPVVSQE
                     WVIQCLIVGERIGFKQHPKYKHDYVSH"
     exon            493..677
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            678..771
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            772..856
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            857..984
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1143
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1273
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..1440
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            1441..1570
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1665
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            1666..1874
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            1875..3201
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            3202..3321
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            3322..3525
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            3526..3583
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            3584..3676
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            3677..4160
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            4161..4313
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            4314..4585
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            4586..5016
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            5017..5208
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            5209..5424
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            5425..5640
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            5641..5735
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            5736..5935
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            5936..6081
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     exon            6082..10591
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6408..6413
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /note="hexamer: AATAAA"
     polyA_site      6438
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /note="major polyA site"
     regulatory      10570..10575
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
                     /note="hexamer: AATAAA"
     polyA_site      10591
                     /gene="TP53BP1"
                     /gene_synonym="53BP1; p202; p53BP1; TDRD30"
ORIGIN      
        1 ggcaattccg cagcctcgcc cgaactgagg ggagccatct tgtccctctc tgcctccgag
       61 tttgtatcgt tgcctccatc tgtgtctgtg tgtctgtatt atcgttgttt ggcgtgtttt
      121 tttttttttt tgttttttgt cactgcctgc ctgggtcctg cccgaggtct ccatcctcgg
      181 tttccctgtc cttgccccgg gccctgggag tgctctggaa ggctgcgcag tattggaggg
      241 gacagaatga ccttccggcc ttgagtccct gggcactctg ccgcagctac ctatcatcca
      301 cgggatttct tgagtggcgg gcggcggcag cgaatggacg atccgatatt gtgtgacgtg
      361 acgggaaagg gggagttcgc ggccggtggc ggcggtggcg acagcggcga cctagggatc
      421 gatctggagg gacttgggga gcgtgcagag acctctagct cgagcgcgag ggacctcccg
      481 ccgggatgcc tggggagcag atggacccta ctggaagtca gttggattca gatttctctc
      541 agcaagatac tccttgcctg ataattgaag attctcagcc tgaaagccag gttctagagg
      601 atgattctgg ttctcacttc agtatgctat ctcgacacct tcctaatctc cagacgcaca
      661 aagaaaatcc tgtgttggat gttgtgtcca atcctgaaca aacagctgga gaagaacgag
      721 gagacggtaa tagtgggttc aatgaacatt tgaaagaaaa caaggttgca gaccctgtgg
      781 attcttctaa cttggacaca tgtggttcca tcagtcaggt cattgagcag ttacctcagc
      841 caaacaggac aagcagtgtt ctgggaatgt cagtggaatc tgctcctgct gtggaggaag
      901 agaagggaga agagttggaa cagaaggaga aagagaagga agaagatact tcaggcaata
      961 ctacacattc ccttggtgct gaagatactg cctcatcaca gttgggtttt ggggttctgg
     1021 aactctccca gagccaggat gttgaggaaa atactgtgcc atatgaagtg gacaaagagc
     1081 agctacaatc agtaaccacc aactctggtt ataccaggct gtctgatgtg gatgctaata
     1141 ctgcaattaa gcatgaagaa cagtccaacg aagatatccc catagcagaa cagtccagca
     1201 aggacatccc tgtgacagca cagcccagta aggatgtaca tgttgtaaaa gagcaaaatc
     1261 caccacctgc aaggtcagag gacatgcctt ttagccccaa agcatctgtt gctgctatgg
     1321 aagcaaaaga acagttgtct gcacaagaac ttatggaaag tggactgcag attcagaagt
     1381 caccagagcc tgaggttttg tcaactcagg aagacttgtt tgaccagagc aataaaacag
     1441 tatcttctga tggttgctct actccttcaa gggaggaagg tgggtgttct ttggcttcca
     1501 ctcctgccac cactctgcat ctcctgcagc tctctggtca gaggtccctt gttcaggaca
     1561 gtctttccac gaattcttca gatcttgttg ctccttctcc tgatgctttc cgatctactc
     1621 cttttatcgt tcctagcagt cccacagagc aagaagggag acaagataag ccaatggaca
     1681 cgtcagtgtt atctgaagaa ggaggagagc cttttcagaa gaaacttcaa agtggtgaac
     1741 cagtggagtt agaaaacccc cctctcctgc ctgagtccac tgtatcacca caagcctcaa
     1801 caccaatatc tcagagcaca ccagtcttcc ctcctgggtc acttcctatc ccatcccagc
     1861 ctcagttttc tcatgacatt tttattcctt ccccaagtct ggaagaacaa tcaaatgatg
     1921 ggaagaaaga tggagatatg catagttcat ctttgacagt tgagtgttct aaaacttcag
     1981 agattgaacc aaagaattcc cctgaggatc ttgggctatc tttgacaggg gattcttgca
     2041 agttgatgct ttctacaagt gaatatagtc agtccccaaa gatggagagc ttgagttctc
     2101 acagaattga tgaagatgga gaaaacacac agattgagga tacggaaccc atgtctccag
     2161 ttctcaattc taaatttgtt cctgctgaaa atgatagtat cctgatgaat ccagcacagg
     2221 atggtgaagt acaactgagt cagaatgatg acaaaacaaa gggagatgat acagacacca
     2281 gggatgacat tagtatttta gccactggtt gcaagggcag agaagaaacg gtagcagaag
     2341 atgtttgtat tgatctcact tgtgattcgg ggagtcaggc agttccgtca ccagctactc
     2401 gatctgaggc actttctagt gtgttagatc aggaggaagc tatggaaatt aaagaacacc
     2461 atccagagga ggggtcttca gggtctgagg tggaagaaat ccctgagaca ccttgtgaaa
     2521 gtcaaggaga ggaactcaaa gaagaaaata tggagagtgt tccgttgcac ctttctctga
     2581 ctgaaactca gtcccaaggg ttgtgtcttc aaaaggaaat gccaaaaaaa gaatgctcag
     2641 aagctatgga agttgaaacc agtgtgatta gtattgattc ccctcaaaag ttggcaatac
     2701 ttgaccaaga attggaacat aaggaacagg aagcttggga agaagctact tcagaggact
     2761 ccagtgttgt cattgtagat gtgaaagagc catctcccag agttgatgtt tcttgtgaac
     2821 ctttggaggg agtggagaag tgctcagatt cccagtcatg ggaggatatt gctccagaaa
     2881 tagaaccatg tgctgagaat agattagaca ccaaggaaga aaagagtgta gaatatgaag
     2941 gagatctgaa atcagggact gcagaaacag aacctgtaga gcaagattct tcacagcctt
     3001 ccttaccttt agtgagagca gatgatcctt taagacttga ccaggagttg cagcagcccc
     3061 aaactcagga gaaaacaagt aattcattaa cagaagactc aaaaatggct aatgcaaagc
     3121 agctaagctc agatgcagag gcccagaagc tggggaagcc ctctgcccat gcctcacaaa
     3181 gcttctgtga aagttctagt gaaaccccat ttcatttcac tttgcctaaa gaaggtgata
     3241 tcatcccacc attgactggt gcaaccccac ctcttattgg gcacctaaaa ttggagccca
     3301 agagacacag tactcctatt ggtattagca actatccaga aagcaccata gcaaccagtg
     3361 atgtcatgtc tgaaagcatg gtggagaccc atgatcccat acttgggagt ggaaaagggg
     3421 attctggggc tgccccagac gtggatgata aattatgtct aagaatgaaa ctggttagtc
     3481 ctgagactga ggcgagtgaa gagtctttgc agttcaacct ggaaaagcct gcaactggtg
     3541 aaagaaaaaa tggatctact gctgttgctg agtctgttgc cagtccccag aagaccatgt
     3601 ctgtgttgag ctgtatctgt gaagccaggc aagagaatga ggctcgaagt gaggatcccc
     3661 ccaccacacc catcaggggg aacttgctcc actttccaag ttctcaagga gaagaggaga
     3721 aagaaaaatt ggagggtgac catacaatca ggcagagtca acagcctatg aagcccatta
     3781 gtcctgtcaa ggaccctgtt tctcctgctt cccagaagat ggtcatacaa gggccatcca
     3841 gtcctcaagg agaggcaatg gtgacagatg tgctagaaga ccagaaagaa ggacggagta
     3901 ctaataagga aaatcctagt aaggccttga ttgaaaggcc cagccaaaat aacataggaa
     3961 tccaaaccat ggagtgttcc ttgagggtcc cagaaactgt ttcagcagca acccagacta
     4021 taaagaatgt gtgtgagcag gggaccagta cagtggacca gaactttgga aagcaagatg
     4081 ccacagttca gactgagagg gggagtggtg agaaaccagt cagtgctcct ggggatgata
     4141 cagagtcgct ccatagccag ggagaagaag agtttgatat gcctcagcct ccacatggcc
     4201 atgtcttaca tcgtcacatg agaacaatcc gggaagtacg cacacttgtc actcgtgtca
     4261 ttacagatgt gtattatgtg gatggaacag aagtagaaag aaaagtaact gaggagactg
     4321 aagagccaat tgtagagtgt caggagtgtg aaactgaagt ttccccttca cagactgggg
     4381 gctcctcagg tgacctgggg gatatcagct ccttctcctc caaggcatcc agcttacacc
     4441 gcacatcaag tgggacaagt ctctcagcta tgcacagcag tggaagctca gggaaaggag
     4501 ccggaccact cagagggaaa accagcggga cagaacccgc agattttgcc ttacccagct
     4561 cccgaggagg cccaggaaaa ctgagagaaa cagctgtgcc tggccccttg ggcatagagg
     4621 acatttcacc taacttgtca ccagatgata aatccttcag ccgtgtcgtg ccccgagtgc
     4681 cagactccac cagacgaaca gatgtgggtg ctggtgcttt gcgtcgtagt gactctccag
     4741 aaattccttt ccaggctgct gctggccctt ctgatggctt agatgcctcc tctccaggaa
     4801 atagctttgt agggctccgt gttgtagcca agtggtcatc caatggctac ttttactctg
     4861 ggaaaatcac acgagatgtc ggagctggga agtataaatt gctctttgat gatgggtacg
     4921 aatgtgatgt gttgggcaaa gacattctgt tatgtgaccc catcccgctg gacactgaag
     4981 tgacggccct ctcggaggat gagtatttca gtgcaggagt ggtgaaagga cataggaagg
     5041 agtctgggga actgtactac agcattgaaa aagaaggcca aagaaagtgg tataagcgaa
     5101 tggctgtcat cctgtccttg gagcaaggaa acagactgag agagcagtat gggcttggcc
     5161 cctatgaagc agtaacacct cttacaaagg cagcagatat cagcttagac aatttggtgg
     5221 aagggaagcg gaaacggcgc agtaacgtca gctccccagc cacccctact gcctccagta
     5281 gcagcagcac aacccctacc cgaaagatca cagaaagtcc tcgtgcctcc atgggagttc
     5341 tctcaggcaa aagaaaactt atcacttctg aagaggaacg gtcccctgcc aagcgaggtc
     5401 gcaagtctgc cacagtaaaa cctggtgcag taggggcagg agagtttgtg agcccctgtg
     5461 agagtggaga caacaccggt gaaccctctg ccctggaaga gcagagaggg cctttgcctc
     5521 tcaacaagac cttgtttctg ggctacgcat ttctccttac catggccaca accagtgaca
     5581 agttggccag ccgctccaaa ctgccagatg gtcctacagg aagcagtgaa gaagaggagg
     5641 aatttttgga aattcctcct ttcaacaagc agtatacaga atcccagctt cgagcaggag
     5701 ctggctatat ccttgaagat ttcaatgaag cccagtgtaa cacagcttac cagtgtcttc
     5761 taattgcgga tcagcattgt cgaacccgga agtacttcct gtgccttgcc agtgggattc
     5821 cttgtgtgtc tcatgtctgg gtccatgata gttgccatgc caaccagctc cagaactacc
     5881 gtaattatct gttgccagct gggtacagcc ttgaggagca aagaattctg gactggcaac
     5941 cccgtgaaaa tcctttccag aatctgaagg tactcttggt atcagaccaa cagcagaact
     6001 tcctggagct ctggtctgag atcctcatga ctggtggtgc agcctctgtg aagcagcacc
     6061 attcaagtgc ccataacaaa gatattgctt taggggtatt tgatgtggtg gtgacggacc
     6121 cctcatgccc agcctcggtg ctgaagtgtg ctgaagcatt gcagctgcct gtggtgtcac
     6181 aagagtgggt gatccagtgc ctcattgttg gggagagaat tggattcaag cagcatccaa
     6241 aatataaaca cgattatgtt tctcactaaa gatacttggt cttactggtt ttattccctg
     6301 ctatcgtgga gattgtgttt taaccaggtt ttaaatgtgt cttgtgtgta actggattcc
     6361 ttgcatggat cttgtatata gttttatttg ctgaactttt atgataaaat aaatgttgaa
     6421 tctctttggt tgtagtaact gggatttctt catctgtttt tttgagctta atctcagaac
     6481 aaatgacaag acatagtact ttctctgagt ctttcaacag gcttattcac ttacggagga
     6541 cagctggcca aggaaattga aaagttaaga gtgaacttta ttctgtggca tcattcccaa
     6601 aaggttattc cagggtgtct aaaatgctat gcttgcagaa actcagttta aggtaggtga
     6661 aggcccagat taacagttgt gccaaaagtt gagtggaatt gggcacagct ctgtttcctg
     6721 acagttaaaa aagacctcat gctctctctc tgagctgaga tcacagctca cctgtgggta
     6781 ctccccaact cttagagcta aagggagaac gaaaggacca actgccatga agggacagtg
     6841 accataagct tgatggaatg accttccgta agataaacat gggaagcaca agtgagaaca
     6901 cctggaaatg ttacacgttc tagtcaaaga cccaatatta ttattattat tattgtcaca
     6961 atagctggaa gcagttcctt cccttcctct ggcatcactg atccctgcat ggcttctcat
     7021 tctctaaagc aggggtcaac aaggtttttt tctgtaaagg gtcaaagagt aaatatttca
     7081 ggctttgtgg gccatttgat ccatcacaac tactcgcctt tgctgtgagg gcatgaaagc
     7141 aaccatagac aatgagtaaa caaatgggca tggctgtgtt tcagtaaaac tgtacaaaaa
     7201 cagacagcag gccatagttt gccagctcct gctccagaga cagcagtaga aagggtgatc
     7261 tttagttgat aatagcaggg aataagttgt cagagcttcc cagtgtgtgt agaatatgta
     7321 gtgatgaaaa ccagatgcag tgactataac ctgatgccag aacactgcat tctttttcag
     7381 tttggagggc gttgttcagt gaatatttct ttttacttac actgatatga atattgatta
     7441 ccagtgatgg ctgggccata ttaagataac ttcaacccct atggtttgtg taagatgggt
     7501 aattgggcct gcaatcttca gtatttaaaa atctaacaac ttgatctcaa ttttttctta
     7561 aggacctttt tcttggagaa taatactttt tttttttttt tttttttttt tgagacggaa
     7621 tttcgctctt gttgcccggg ctggaatgca atggcacaat ctcagctcac tgcagcgtct
     7681 gcttcccagg ttcaagcaat tctcctgtct cagcctcctg agtagctggg attacaggca
     7741 catgccacca cacctggcta atttttgtat ttttagtaga atcgaggttt catcatgttg
     7801 gtcaggctgg tctcaaactc ctgacttcag gtgatccgcc cgcctcggcc tcccaaagtg
     7861 ctgggattac aggtgtgagc caccatgccc ggcctaagaa atacttttaa gtatattttc
     7921 attagctaga attgcccaat ctgtgtaggt ataaattact tggtataggg agagagaaag
     7981 cctatcttac ctgttgcttt cttacttggt ggtaacatcc agcagttagt ctatttataa
     8041 acataattac tttttcacat atgaaccata aaatatttaa ctttctgctc tatattgttt
     8101 gtttaccgct gtatctccca cagcttgaac agtaccaagg tacgtagtag gtgctcaata
     8161 aatgactatt gaataaatga acatatccaa caaatgttct caatgtaaag gatcagagat
     8221 gccacatgtt ctccttgatg ggagagaccc ttccacatgg gaatgatggg aaggagttgt
     8281 actcctggat gttcagtaac tgcttctagg agaaaaggta gagtcctatc actaagccgc
     8341 agatatttat ttgtgtgtgg ctagaatggg atgttttgaa tcttctgtta caaccttggg
     8401 aacgtggctg ttatttcaat ttatgagcca gaaattttca catcccgaaa ctacaaaaga
     8461 gaaaaagagc cttattaagt gtcatgcttt cccaagacta ccttcaaaga aatatgaatc
     8521 aggataacct gtgatctaaa taatgtcatc ttaaaactga agagtttctt ttgactcttc
     8581 tgctacaata gcttagaaaa aaatctgctt gcagacattt tagagagaaa ggacaatgaa
     8641 gtgattttct gaatgggaat gacagacctc tgggaagcca gctaccactg aatctcggta
     8701 tcagtttttt ttaaagttta gagttagaag gggtggtcgc ctcctttcac agatgcggaa
     8761 gctagggacc agcaaggcgg ggtgcccacg gctgcacagc tagttcatat cagaattggg
     8821 agtggaaggc ccactgcctc ccagcatagc aatacataac ctagcaaagg acttaacacc
     8881 tatctcactg tcaggttttt tagtatttta tgatgatgat gacttctact agaaaataac
     8941 ctccattaaa attattaaag atggtcacac ctctatctct aagccttact tataaaatga
     9001 gggtatttgg actaaagtct tcttccagtt ctagaattct acaactcatt aaaaagccac
     9061 cttaaaaagt ctactgagtt acccaagggt tgctcctacc tgcccagagt tccaccagcc
     9121 tgggtatagt atttgttata atctagtcgt aacagtagtt gagccaaatc tgagttgatc
     9181 tgatgattcc gaacactgga gagaatcttg aacaggagtg aagactggcg gctaaagccc
     9241 tgcagagaga aggactcagc tgtcattcca cttcagctca ccaactctcc atatggagga
     9301 tgggggcgga gggaggagtt tcttggaaaa gccttgttca aaattctaca gaaccacctg
     9361 gccttcccac attcctattt ctattagttc ctaaaatgac ttgtaccaaa tccatacatg
     9421 catgacttcc tatgaaagta ctctttcatc agtaggaatt tagtagctgg tttccagtta
     9481 atgtattttg tcaagtactg gggttgggga gaacccgttt tgattacaag cagataatta
     9541 tctcagtgag atgggggtta gttcaaggaa gtaaggaggg gggaggatgt gaggaagtta
     9601 gaacaaccca atgcttattt gatgggctga ataaactatt caggactgaa ctatttttga
     9661 gcactgtgag gtggcacagt attacctgct tcaaaatcaa ctgataccaa catttttatc
     9721 tttgtatctt atctctgtac gtgtgtgtat tgaggaaatg ctttactgac tcagaggaaa
     9781 gatcatgaat tctccattgg caaaaccacc tctgtccttt cggcaaggct gcatacttcc
     9841 aggcagacgc accttcacga gaatgctcag ctgggcggct ccacgctcat ccagtgggcc
     9901 taggttctga ctgaccagcg aacaaaaact gtgacagaga tctaggattt cattcaggca
     9961 gtgaaacacc tacccgggaa acagagttgg cattaggaaa ggaaggaagg tacatccatg
    10021 aagttaaagt gttaggagaa cagtctgatt aatagctgat ctaattaata gctgacctcc
    10081 caaatctgac aggatagaca ctgccacgtg caaggcctgc cagcccctca gacgcacaaa
    10141 atgcgtaaaa caaatgcatc ctttcctggc taagcgagta ttactctctt agccctgcac
    10201 caaacctcca atctagccac atttaactct tcatttctta gacccgcaga gtgtcttcct
    10261 gcctctgagc tgtgagtgtt gttccctttg cccgggatgc tcttgttttt aataccagtt
    10321 caagtcccac tctctcagtg aagcactccc ttccccacta tagcctttag tgaaccctcg
    10381 tttcttgctt ctttattatc tgtactgttg tccacttggc aattgttcag gcctctgtgt
    10441 tgttactgat ttttgtatgt atatatatat atatgtcttg tttttccaac tagattgtga
    10501 gctccttaag ggcagagcca tgaattatac ctctttgtat ccccagtgcc ttgcatacag
    10561 taagcactca ataaatattt aatgatgatg a
//
