LOCUS       NP_008958               1522 aa            linear   PRI 09-FEB-2025
DEFINITION  DNA topoisomerase 2-binding protein 1 isoform 1 [Homo sapiens].
ACCESSION   NP_008958
VERSION     NP_008958.2
DBSOURCE    REFSEQ: accession NM_007027.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 1522)
  AUTHORS   Egger,T., Morano,L., Blanchard,M.P., Basbous,J. and Constantinou,A.
  TITLE     Spatial organization and functions of Chk1 activation by TopBP1
            biomolecular condensates
  JOURNAL   Cell Rep 43 (4), 114064 (2024)
   PUBMED   38578830
  REMARK    GeneRIF: Spatial organization and functions of Chk1 activation by
            TopBP1 biomolecular condensates.
REFERENCE   2  (residues 1 to 1522)
  AUTHORS   Sun,X., Dong,M., Li,J., Sun,Y., Gao,Y., Wang,Y., Du,L., Liu,Y.,
            Ji,K., He,N., Wang,J., Zhang,M., Song,H., Xu,C. and Liu,Q.
  TITLE     NRF2 promotes radiation resistance by cooperating with TOPBP1 to
            activate the ATR-CHK1 signaling pathway
  JOURNAL   Theranostics 14 (2), 681-698 (2024)
   PUBMED   38169561
  REMARK    GeneRIF: NRF2 promotes radiation resistance by cooperating with
            TOPBP1 to activate the ATR-CHK1 signaling pathway.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 1522)
  AUTHORS   Prabhakar,A.T., James,C.D., Fontan,C.T., Otoa,R., Wang,X.,
            Bristol,M.L., Yeager,C., Hill,R.D., Dubey,A., Wu,S.-Y.,
            Chiang,C.-M. and Morgan,I.M.
  TITLE     Direct interaction with the BRD4 carboxyl-terminal motif (CTM) and
            TopBP1 is required for human papillomavirus 16 E2 association with
            mitotic chromatin and plasmid segregation function
  JOURNAL   J Virol 97 (10), e0078223 (2023)
   PUBMED   37712702
  REMARK    GeneRIF: Direct interaction with the BRD4 carboxyl-terminal motif
            (CTM) and TopBP1 is required for human papillomavirus 16 E2
            association with mitotic chromatin and plasmid segregation
            function.
REFERENCE   4  (residues 1 to 1522)
  AUTHORS   Gelot,C., Kovacs,M.T., Miron,S., Mylne,E., Haan,A.,
            Boeffard-Dosierre,L., Ghouil,R., Popova,T., Dingli,F., Loew,D.,
            Guirouilh-Barbat,J., Del Nery,E., Zinn-Justin,S. and Ceccaldi,R.
  TITLE     Poltheta; is phosphorylated by PLK1 to repair double-strand breaks
            in mitosis
  JOURNAL   Nature 621 (7978), 415-422 (2023)
   PUBMED   37674080
  REMARK    Erratum:[Nature. 2024 Feb;626(7999):E13. doi:
            10.1038/s41586-024-07025-8.. PMID: 38273133]
REFERENCE   5  (residues 1 to 1522)
  AUTHORS   Trivedi,P., Steele,C.D., Au,F.K.C., Alexandrov,L.B. and
            Cleveland,D.W.
  TITLE     Mitotic tethering enables inheritance of shattered micronuclear
            chromosomes
  JOURNAL   Nature 618 (7967), 1049-1056 (2023)
   PUBMED   37316668
REFERENCE   6  (residues 1 to 1522)
  AUTHORS   Honda,Y., Tojo,M., Matsuzaki,K., Anan,T., Matsumoto,M., Ando,M.,
            Saya,H. and Nakao,M.
  TITLE     Cooperation of HECT-domain ubiquitin ligase hHYD and DNA
            topoisomerase II-binding protein for DNA damage response
  JOURNAL   J Biol Chem 277 (5), 3599-3605 (2002)
   PUBMED   11714696
REFERENCE   7  (residues 1 to 1522)
  AUTHORS   Makiniemi,M., Hillukkala,T., Tuusa,J., Reini,K., Vaara,M.,
            Huang,D., Pospiech,H., Majuri,I., Westerling,T., Makela,T.P. and
            Syvaoja,J.E.
  TITLE     BRCT domain-containing protein TopBP1 functions in DNA replication
            and damage response
  JOURNAL   J Biol Chem 276 (32), 30399-30406 (2001)
   PUBMED   11395493
REFERENCE   8  (residues 1 to 1522)
  AUTHORS   Potter,T., Gohde,W., Wedemeyer,N. and Kohnlein,W.
  TITLE     Keratinocytes exposed to ultraviolet radiation reveal three
            down-regulated genes with potential function in differentiation and
            cell cycle control
  JOURNAL   Radiat Res 154 (2), 151-158 (2000)
   PUBMED   10931686
REFERENCE   9  (residues 1 to 1522)
  AUTHORS   Yamane,K. and Tsuruo,T.
  TITLE     Conserved BRCT regions of TopBP1 and of the tumor suppressor BRCA1
            bind strand breaks and termini of DNA
  JOURNAL   Oncogene 18 (37), 5194-5203 (1999)
   PUBMED   10498869
REFERENCE   10 (residues 1 to 1522)
  AUTHORS   Yamane,K., Kawabata,M. and Tsuruo,T.
  TITLE     A DNA-topoisomerase-II-binding protein with eight repeating regions
            similar to DNA-repair enzymes and to a cell-cycle regulator
  JOURNAL   Eur J Biochem 250 (3), 794-799 (1997)
   PUBMED   9461304
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC083905.20, BC032633.1,
            BC126209.1, BC151237.1 and AC016255.24.
            
            On Jul 25, 2008 this sequence version replaced NP_008958.1.
            
            Summary: This gene encodes a binding protein which interacts with
            the C-terminal region of topoisomerase II beta. This interaction
            suggests a supportive role for this protein in the catalytic
            reactions of topoisomerase II beta through transient breakages of
            DNA strands. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC151237.1, D87448.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000260810.10/ ENSP00000260810.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q22.1"
     Protein         1..1522
                     /product="DNA topoisomerase 2-binding protein 1 isoform 1"
                     /note="DNA topoisomerase 2-binding protein 1; DNA
                     topoisomerase II-beta-binding protein 1; topoisomerase
                     (DNA) II binding protein 1"
                     /calculated_mol_wt=170548
     Region          108..179
                     /region_name="BRCT_TopBP1_rpt1"
                     /note="first BRCT domain of DNA topoisomerase 2-binding
                     protein 1 (TopBP1) and similar proteins; cd17737"
                     /db_xref="CDD:349369"
     Region          198..274
                     /region_name="BRCT_TopBP1_rpt2_like"
                     /note="second BRCT domain of DNA topoisomerase 2-binding
                     protein 1 (TopBP1) and similar proteins; cd17731"
                     /db_xref="CDD:349363"
     Site            order(207..211,215,231..235,249..250,253,257..258)
                     /site_type="other"
                     /note="Crb2 interaction site [polypeptide binding]"
                     /db_xref="CDD:349363"
     Site            298
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q92547.3)"
     Site            301
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:17525332; propagated from
                     UniProtKB/Swiss-Prot (Q92547.3)"
     Region          353..434
                     /region_name="BRCT_TopBP1_rpt3"
                     /note="third BRCT domain of DNA topoisomerase 2-binding
                     protein 1 (TopBP1) and similar proteins; cd17718"
                     /db_xref="CDD:349350"
     Region          553..636
                     /region_name="BRCT_TopBP1_rpt4"
                     /note="fourth BRCT domain of DNA topoisomerase 2-binding
                     protein 1 (TopBP1) and similar proteins; cd17749"
                     /db_xref="CDD:349380"
     Region          645..733
                     /region_name="BRCT_TopBP1_rpt5"
                     /note="fifth BRCT domain of DNA topoisomerase 2-binding
                     protein 1 (TopBP1) and similar proteins; cd18434"
                     /db_xref="CDD:349387"
     Region          756..891
                     /region_name="Interaction with CIP2A.
                     /evidence=ECO:0000269|PubMed:35121901,
                     ECO:0000269|PubMed:35842428"
                     /note="propagated from UniProtKB/Swiss-Prot (Q92547.3)"
     Site            779
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:18669648,
                     ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q92547.3)"
     Site            848
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:18669648; propagated from
                     UniProtKB/Swiss-Prot (Q92547.3)"
     Region          852..858
                     /region_name="Nuclear localization signal.
                     /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q92547.3)"
     Site            860
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q92547.3)"
     Site            861
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q92547.3)"
     Site            864
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q92547.3)"
     Site            886
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:20068231; propagated from
                     UniProtKB/Swiss-Prot (Q92547.3)"
     Site            888
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:16964243,
                     ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332,
                     ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163;
                     propagated from UniProtKB/Swiss-Prot (Q92547.3)"
     Region          905..979
                     /region_name="BRCT_TopBP1_rpt6"
                     /note="sixth BRCT domain of DNA topoisomerase 2-binding
                     protein 1 (TopBP1) and similar proteins; cd17727"
                     /db_xref="CDD:349359"
     Site            1002
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:18669648; propagated from
                     UniProtKB/Swiss-Prot (Q92547.3)"
     Region          1018..1058
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q92547.3)"
     Site            1062
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000269|PubMed:21777809; propagated from
                     UniProtKB/Swiss-Prot (Q92547.3)"
     Site            1064
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q92547.3)"
     Region          1083..1118
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q92547.3)"
     Region          1267..1341
                     /region_name="BRCT_TopBP1_rpt7"
                     /note="seventh BRCT domain of DNA topoisomerase 2-binding
                     protein 1; cd17738"
                     /db_xref="CDD:349370"
     Site            order(1272..1276,1280,1311,1313..1317)
                     /site_type="other"
                     /note="BACH1 interaction site [polypeptide binding]"
                     /db_xref="CDD:349370"
     Region          1396..1476
                     /region_name="BRCT_TopBP1_rpt8"
                     /note="eighth (C-terminal) BRCT domain of DNA
                     topoisomerase 2-binding protein 1; cd17728"
                     /db_xref="CDD:349360"
     Region          1501..1522
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q92547.3)"
     Site            1504
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q92547.3)"
     Region          1517..1520
                     /region_name="Nuclear localization signal.
                     /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q92547.3)"
     CDS             1..1522
                     /gene="TOPBP1"
                     /gene_synonym="Dpb11; TOP2BP1"
                     /coded_by="NM_007027.4:181..4749"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS46919.1"
                     /db_xref="GeneID:11073"
                     /db_xref="HGNC:HGNC:17008"
                     /db_xref="MIM:607760"
ORIGIN      
        1 msrndkepff vkflkssdns kcffkalesi kefqseeylq iiteeealki kendrslyic
       61 dpfsgvvfdh lkklgcrivg pqvvifcmhh qrcvpraehp vynmvmsdvt isctslekek
      121 reevhkyvqm mggrvyrdln vsvthliage vgskkylvaa nlkkpillps wiktlweksq
      181 ekkitrytdi nmedfkcpif lgciicvtgl cgldrkevqq ltvkhggqym gqlkmnecth
      241 livqepkgqk yecakrwnvh cvttqwffds iekgfcqdes iykteprpea ktmpnsstpt
      301 sqintidsrt lsdvsnisni nascvsesic nslnsklept lenlenldvs afqapedlld
      361 gcriylcgfs grkldklrrl insgggvrfn qlnedvthvi vgdyddelkq fwnksahrph
      421 vvgakwllec fskgymlsee pyihanyqpv eipvshkpes kaallkkkns sfskkdfaps
      481 ekheqadedl lsqyengsst vveaktsear pfndsthaep lndsthislq eenqssvshc
      541 vpdvstitee glfsqksflv lgfsnenesn ianiikenag kimsllsrtv adyavvpllg
      601 ceveatvgev vtntwlvtci dyqtlfdpks nplftpvpvm tgmtpledcv isfsqcagae
      661 kesltflanl lgasvqeyfv rksnakkgmf asthlilker ggskyeaakk wnlpavtiaw
      721 lletartgkr adeshflien stkeerslet eitnginlns dtaehpgtrl qthrktvvtp
      781 ldmnrfqska fravvsqhar qvaaspavgq plqkepslhl dtpskflskd klfkpsfdvk
      841 dalaaletpg rpsqqkrkps tplsevivkn lqlalanssr navalsaspq lkeaqsekee
      901 apkplhkvvv cvskklskkq selngiaasl gadyrwsfde tvthfiyqgr pndtnreyks
      961 vkergvhivs ehwlldcaqe ckhlpeslyp htynpkmsld isavqdgrlc nsrllsavss
     1021 tkddepdpli leendvdnma tnnkesapsn gsgkndskgv ltqtlemren fqkqlqeims
     1081 atsivkpqgq rtslsrsgcn sasstpdstr sarsgrsrvl ealrqsrqtv pdvntepsqn
     1141 eqiiwddpta reerarlasn lqwpscptqy selqvdiqnl edspfqkplh dseiakqavc
     1201 dpgnirvtea pkhpiseele tpikdshlip tpqapsiafp lanppvaphp rekiitieet
     1261 heelkkqyif qlsslnpqer idychliekl gglviekqcf dptcthivvg hplrnekyla
     1321 svaagkwvlh rsyleacrta ghfvqeedye wgsssildvl tginvqqrrl alaamrwrkk
     1381 iqqrqesgiv egafsgwkvi lhvdqsreag fkrllqsgga kvlpghsvpl fkeathlfsd
     1441 lnklkpddsg vniaeaaaqn vyclrteyia dylmqespph venyclpeai sfiqnnkelg
     1501 tglsqkrkap teknkikrpr vh
//
