LOCUS       NM_003935               3150 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant
            1, mRNA.
ACCESSION   NM_003935
VERSION     NM_003935.5
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Yang,X., Saha,S., Yang,W., Neuman,K.C. and Pommier,Y.
  TITLE     Structural and biochemical basis for DNA and RNA catalysis by human
            Topoisomerase 3beta
  JOURNAL   Nat Commun 13 (1), 4656 (2022)
   PUBMED   35945419
  REMARK    GeneRIF: Structural and biochemical basis for DNA and RNA catalysis
            by human Topoisomerase 3beta.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Su,S., Xue,Y., Sharov,A., Zhang,Y., Lee,S.K., Martindale,J.L.,
            Li,W., Ku,W.L., Zhao,K., De,S., Shen,W., Sen,P., Gorospe,M., Xu,D.
            and Wang,W.
  TITLE     A dual-activity topoisomerase complex regulates mRNA translation
            and turnover
  JOURNAL   Nucleic Acids Res 50 (12), 7013-7033 (2022)
   PUBMED   35748872
  REMARK    GeneRIF: A dual-activity topoisomerase complex regulates mRNA
            translation and turnover.
REFERENCE   3  (bases 1 to 3150)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 3150)
  AUTHORS   Saha,S., Sun,Y., Huang,S.N., Baechler,S.A., Pongor,L.S., Agama,K.,
            Jo,U., Zhang,H., Tse-Dinh,Y.C. and Pommier,Y.
  TITLE     DNA and RNA Cleavage Complexes and Repair Pathway for TOP3B RNA-
            and DNA-Protein Crosslinks
  JOURNAL   Cell Rep 33 (13), 108569 (2020)
   PUBMED   33378676
  REMARK    GeneRIF: DNA and RNA Cleavage Complexes and Repair Pathway for
            TOP3B RNA- and DNA-Protein Crosslinks.
REFERENCE   5  (bases 1 to 3150)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   6  (bases 1 to 3150)
  AUTHORS   Oliveira-Costa,J.P., Zanetti,J., Oliveira,L.R., Soares,F.A.,
            Ramalho,L.Z., Silva Ramalho,F., Garcia,S.B. and Ribeiro-Silva,A.
  TITLE     Significance of topoisomerase IIIbeta expression in breast ductal
            carcinomas: strong associations with disease-specific survival and
            metastasis
  JOURNAL   Hum Pathol 41 (11), 1624-1630 (2010)
   PUBMED   20950730
  REMARK    GeneRIF: Topoisomerase IIIbeta expression can be a useful marker in
            assessing the prognosis of patients with breast cancer and is an
            independent predictor of survival.
            Erratum:[Hum Pathol. 2012 Apr;43(4):618]
REFERENCE   7  (bases 1 to 3150)
  AUTHORS   Cho,Y.H., Park,J.Y., Han,S.Y. and Chung,I.K.
  TITLE     Identification of the functional elements in the promoter region of
            human DNA topoisomerase IIIbeta gene
  JOURNAL   Biochim Biophys Acta 1679 (3), 272-278 (2004)
   PUBMED   15358519
  REMARK    GeneRIF: the positive regulatory region including the 5'GGAACC3'
            core element is essential for efficient expression of the DNA
            topoisomerase III beta gene
REFERENCE   8  (bases 1 to 3150)
  AUTHORS   Kobayashi,M. and Hanai,R.
  TITLE     M phase-specific association of human topoisomerase IIIbeta with
            chromosomes
  JOURNAL   Biochem Biophys Res Commun 287 (1), 282-287 (2001)
   PUBMED   11549288
  REMARK    GeneRIF: Two isoforms of human DNA topoisomerase IIIbeta were
            expressed in HeLa cells. Isoform 1 was localized to the nucleus and
            associated with chromosomes during metaphase and anaphase. Isoform
            2 was found in the cytoplasm.
REFERENCE   9  (bases 1 to 3150)
  AUTHORS   Shimamoto,A., Nishikawa,K., Kitao,S. and Furuichi,Y.
  TITLE     Human RecQ5beta, a large isomer of RecQ5 DNA helicase, localizes in
            the nucleoplasm and interacts with topoisomerases 3alpha and 3beta
  JOURNAL   Nucleic Acids Res 28 (7), 1647-1655 (2000)
   PUBMED   10710432
REFERENCE   10 (bases 1 to 3150)
  AUTHORS   Ng,S.W., Liu,Y., Hasselblatt,K.T., Mok,S.C. and Berkowitz,R.S.
  TITLE     A new human topoisomerase III that interacts with SGS1 protein
  JOURNAL   Nucleic Acids Res 27 (4), 993-1000 (1999)
   PUBMED   9927731
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA025312.1, BC002432.2,
            CR456596.1 and BQ270362.1.
            
            On Jun 2, 2019 this sequence version replaced NM_003935.4.
            
            Summary: This gene encodes a DNA topoisomerase, an enzyme that
            controls and alters the topologic states of DNA during
            transcription. This enzyme catalyzes the transient breaking and
            rejoining of a single strand of DNA which allows the strands to
            pass through one another, thus relaxing the supercoils and altering
            the topology of DNA. The enzyme interacts with DNA helicase SGS1
            and plays a role in DNA recombination, cellular aging and
            maintenance of genome stability. Low expression of this gene may be
            related to higher survival rates in breast cancer patients. This
            gene has a pseudogene on chromosome 22. Alternate splicing results
            in multiple transcript variants. Additional alternatively spliced
            transcript variants of this gene have been described, but their
            full-length nature is not known. [provided by RefSeq, Aug 2013].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1-5 encode the same protein (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.174756.1, BC002432.2
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2158188, SAMEA2162946
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                DA025312.1         57-93
            38-446              BC002432.2         1-409
            447-1565            CR456596.1         2-1120
            1566-3144           BC002432.2         1529-3107
            3145-3150           BQ270362.1         373-378
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q11.22"
     gene            1..3150
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /note="DNA topoisomerase III beta"
                     /db_xref="GeneID:8940"
                     /db_xref="HGNC:HGNC:11993"
                     /db_xref="MIM:603582"
     exon            1..374
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            375..542
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     CDS             473..3061
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /EC_number="5.6.2.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     topoisomerase III beta; DNA topoisomerase 3-beta-1;
                     topoisomerase (DNA) III beta; DNA topoisomerase III
                     beta-1"
                     /codon_start=1
                     /product="DNA topoisomerase 3-beta-1 isoform 1"
                     /protein_id="NP_003926.1"
                     /db_xref="CCDS:CCDS13797.1"
                     /db_xref="GeneID:8940"
                     /db_xref="HGNC:HGNC:11993"
                     /db_xref="MIM:603582"
                     /translation="MKTVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGT
                     FAGQPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFL
                     QVEGRGCDYIVLWLDCDKEGENICFEVLDAVLPVMNKAHGGEKTVFRARFSSITDTDI
                     CNAMACLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQ
                     TPTLGFCVERHDKIQSFKPETYWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNM
                     TKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYI
                     SYPRTETTHYPENFDLKGSLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITP
                     MKSATEAELGGDAWRLYEYITRHFIATVSHDCKYLQSTISFRIGPELFTCSGKTVLSP
                     GFTEVMPWQSVPLEESLPTCQRGDAFPVGEVKMLEKQTNPPDYLTEAELITLMEKHGI
                     GTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEK
                     QLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRC
                     GKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRG
                     KSYPLCPYCYNHPPFRDMKKGMGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTS
                     GPKWKVACNKCNVVAHCFENAHRVRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHM
                     GCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRGRARRPPGKPNPRRPKDKMSA
                     LAAYFV"
     exon            543..674
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            675..781
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            782..856
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            857..1053
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1210
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1324
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1415
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1416..1570
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1676
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1677..1823
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1997
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1998..2126
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            2127..2276
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            2277..2377
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            2378..2579
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            2580..3150
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gttgtcggta gccgccccgg gaacaaggac cggagctgga tccgcggtgc ggctgtgggt
       61 gagcggcggg ctgcgggacc ctcgcccggc ggctcagaaa ggcgggaagc gcacgcattt
      121 ttctggcttg tttcctgaac gttggccggc ggaggacccg cgagctcggg agagcgcccg
      181 ggccgggctc tgcattcttg ttgcctcttc cctggcgggg gtgatactct cccgcggggc
      241 ttgctgtggg ggttcaggga ggtggagatg ggcccgccat gtgcctgcag ggagcaggcg
      301 ctgtggttaa tacttttctt cttcgaaccc tcccacagct ctttagggtg agatctgctg
      361 taccagtctc acaggagtca gcattggtaa tagtgctgtg ctgcggaaga gtggagtcta
      421 aactttttca ttgccagtgc cccgaaactg gagtgtgaag gaccgagaca aaatgaagac
      481 tgtgctcatg gttgctgaaa agccgtcctt ggcacagtca attgccaaaa tcctctctag
      541 agggagcctg tcctcacaca aagggctgaa cggggcctgc tcagtccacg agtacactgg
      601 gacctttgct ggccagccag tgcgcttcaa gatgacgtct gtctgtggtc acgtgatgac
      661 cctggatttc ctgggaaaat acaacaaatg ggacaaagtg gaccccgcag aactgttcag
      721 ccaagctccc acggagaaga aagaagctaa ccccaagctg aacatggtga agttcctgca
      781 ggtggagggc agaggctgcg actacatcgt gctgtggctg gactgcgaca aggaggggga
      841 gaacatctgc tttgaggttc ttgatgctgt tctgcccgtc atgaacaagg cccatggtgg
      901 cgagaagacc gtgttccggg ccaggtttag ctccatcacg gacacagaca tctgtaatgc
      961 catggcctgc ctaggcgagc ctgaccacaa cgaggcgctc tcagtggatg ctcgccagga
     1021 gctggacctg cgaatcggct gtgcattcac caggtttcag actaaatatt tccaggggaa
     1081 atacggtgat ttagacagct ctctcatctc ctttgggccg tgtcagactc caaccctggg
     1141 attctgtgtg gagagacatg ataaaatcca gtccttcaaa ccagagacct actgggtgct
     1201 gcaggccaag gttaacactg acaaagacag atctctcctt ttggactggg accgagtaag
     1261 agtgtttgac cgggagatcg cacagatgtt tttaaacatg acaaagctgg agaaggaagc
     1321 ccaggtggag gccacaagca ggaaagaaaa ggccaagcag aggcccctgg ccctgaacac
     1381 tgtggagatg ctgcgtgtgg ccagctcttc tctgggcatg gggccgcagc acgccatgca
     1441 gacggctgag cggctctaca cgcaaggcta catcagctac ccacggacag agaccaccca
     1501 ctaccctgag aactttgacc tgaagggctc tctgcggcag caggccaacc acccctactg
     1561 ggccgacacg gtgaagcggt tgttagcaga aggtatcaac cgcccgcgga aaggccatga
     1621 cgccggcgac catcccccca tcacccccat gaagtctgcc acagaggccg aattaggggg
     1681 tgacgcgtgg cggctctatg agtacatcac cagacacttc atcgccacgg tcagccatga
     1741 ctgcaagtac ctgcagagca ccatctcctt cagaattggg cccgagctct tcacctgctc
     1801 cgggaagacc gtcctctcac caggcttcac ggaggtcatg ccctggcaga gcgtgcccct
     1861 ggaggagagc ctgcccactt gccagcgggg tgatgccttc cctgtgggcg aggtgaagat
     1921 gctggagaag cagacgaacc cacccgacta cctgacggag gccgagctca tcacgctcat
     1981 ggagaagcat ggcatcggca cggatgccag catccctgtg catatcaaca acatctgcca
     2041 gcgcaactat gtcacggtgg agagcgggcg ccggctcaag cccaccaacc tcggcatcgt
     2101 cctggtgcac ggctactata agattgatgc agagctggtg ctccccacca tccgcagtgc
     2161 agtggagaag cagctgaacc tgatcgccca gggcaaggcc gactaccgcc aggtcctggg
     2221 ccacaccctg gacgtgttca agaggaagtt ccactacttt gtcgactcca ttgctggcat
     2281 ggatgagttg atggaggtgt ctttctcgcc cctggcggcc acaggcaagc ccctctcacg
     2341 ctgtgggaag tgccaccgct tcatgaagta catccaggcc aagccaagcc gcctgcactg
     2401 ctcccactgc gatgagacct acacgctccc ccagaacggc accatcaagc tctacaagga
     2461 gctccgctgc cctctggatg acttcgagct ggtcctgtgg tcatcaggct ctcggggcaa
     2521 gagctacccg ctgtgcccct actgctacaa ccacccaccc ttccgagaca tgaagaaagg
     2581 catgggctgc aacgagtgta cgcacccctc ctgccagcac tcgctgagca tgctgggcat
     2641 cggccagtgc gtggaatgtg agagcggggt gctggtgctg gaccccacct cgggccccaa
     2701 gtggaaggtg gcctgcaaca agtgcaacgt ggtagcgcac tgcttcgaga acgcccaccg
     2761 cgtgcgggtg tccgccgaca cctgcagtgt ctgtgaggcc gccttgcttg atgtggactt
     2821 caacaaggcc aagtccccac tcccgggcga tgagacgcag cacatgggct gcgtcttttg
     2881 tgaccccgtc ttccaggagc tggtggagct gaagcatgcg gcctcctgcc accccatgca
     2941 ccgcggtgga ccagggagaa ggcagggtcg agggcggggc cgggccagga ggccccctgg
     3001 gaagcccaac cccagacggc ccaaggacaa gatgtcagcc ctggccgcct actttgtatg
     3061 atgaccctgt cctccctcac ccaggctgca gtgccatgca gacacctcat ggcacttcaa
     3121 actattaaaa tgcattttat cttatccaga
//
