LOCUS       NM_001349845            2870 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant
            4, mRNA.
ACCESSION   NM_001349845 XM_006724349
VERSION     NM_001349845.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Yang,X., Saha,S., Yang,W., Neuman,K.C. and Pommier,Y.
  TITLE     Structural and biochemical basis for DNA and RNA catalysis by human
            Topoisomerase 3beta
  JOURNAL   Nat Commun 13 (1), 4656 (2022)
   PUBMED   35945419
  REMARK    GeneRIF: Structural and biochemical basis for DNA and RNA catalysis
            by human Topoisomerase 3beta.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Su,S., Xue,Y., Sharov,A., Zhang,Y., Lee,S.K., Martindale,J.L.,
            Li,W., Ku,W.L., Zhao,K., De,S., Shen,W., Sen,P., Gorospe,M., Xu,D.
            and Wang,W.
  TITLE     A dual-activity topoisomerase complex regulates mRNA translation
            and turnover
  JOURNAL   Nucleic Acids Res 50 (12), 7013-7033 (2022)
   PUBMED   35748872
  REMARK    GeneRIF: A dual-activity topoisomerase complex regulates mRNA
            translation and turnover.
REFERENCE   3  (bases 1 to 2870)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2870)
  AUTHORS   Saha,S., Sun,Y., Huang,S.N., Baechler,S.A., Pongor,L.S., Agama,K.,
            Jo,U., Zhang,H., Tse-Dinh,Y.C. and Pommier,Y.
  TITLE     DNA and RNA Cleavage Complexes and Repair Pathway for TOP3B RNA-
            and DNA-Protein Crosslinks
  JOURNAL   Cell Rep 33 (13), 108569 (2020)
   PUBMED   33378676
  REMARK    GeneRIF: DNA and RNA Cleavage Complexes and Repair Pathway for
            TOP3B RNA- and DNA-Protein Crosslinks.
REFERENCE   5  (bases 1 to 2870)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   6  (bases 1 to 2870)
  AUTHORS   Oliveira-Costa,J.P., Zanetti,J., Oliveira,L.R., Soares,F.A.,
            Ramalho,L.Z., Silva Ramalho,F., Garcia,S.B. and Ribeiro-Silva,A.
  TITLE     Significance of topoisomerase IIIbeta expression in breast ductal
            carcinomas: strong associations with disease-specific survival and
            metastasis
  JOURNAL   Hum Pathol 41 (11), 1624-1630 (2010)
   PUBMED   20950730
  REMARK    GeneRIF: Topoisomerase IIIbeta expression can be a useful marker in
            assessing the prognosis of patients with breast cancer and is an
            independent predictor of survival.
            Erratum:[Hum Pathol. 2012 Apr;43(4):618]
REFERENCE   7  (bases 1 to 2870)
  AUTHORS   Cho,Y.H., Park,J.Y., Han,S.Y. and Chung,I.K.
  TITLE     Identification of the functional elements in the promoter region of
            human DNA topoisomerase IIIbeta gene
  JOURNAL   Biochim Biophys Acta 1679 (3), 272-278 (2004)
   PUBMED   15358519
  REMARK    GeneRIF: the positive regulatory region including the 5'GGAACC3'
            core element is essential for efficient expression of the DNA
            topoisomerase III beta gene
REFERENCE   8  (bases 1 to 2870)
  AUTHORS   Kobayashi,M. and Hanai,R.
  TITLE     M phase-specific association of human topoisomerase IIIbeta with
            chromosomes
  JOURNAL   Biochem Biophys Res Commun 287 (1), 282-287 (2001)
   PUBMED   11549288
  REMARK    GeneRIF: Two isoforms of human DNA topoisomerase IIIbeta were
            expressed in HeLa cells. Isoform 1 was localized to the nucleus and
            associated with chromosomes during metaphase and anaphase. Isoform
            2 was found in the cytoplasm.
REFERENCE   9  (bases 1 to 2870)
  AUTHORS   Shimamoto,A., Nishikawa,K., Kitao,S. and Furuichi,Y.
  TITLE     Human RecQ5beta, a large isomer of RecQ5 DNA helicase, localizes in
            the nucleoplasm and interacts with topoisomerases 3alpha and 3beta
  JOURNAL   Nucleic Acids Res 28 (7), 1647-1655 (2000)
   PUBMED   10710432
REFERENCE   10 (bases 1 to 2870)
  AUTHORS   Ng,S.W., Liu,Y., Hasselblatt,K.T., Mok,S.C. and Berkowitz,R.S.
  TITLE     A new human topoisomerase III that interacts with SGS1 protein
  JOURNAL   Nucleic Acids Res 27 (4), 993-1000 (1999)
   PUBMED   9927731
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA025312.1, DA124773.1,
            AK290997.1, AC245452.3 and BQ270362.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001349845.1.
            
            Summary: This gene encodes a DNA topoisomerase, an enzyme that
            controls and alters the topologic states of DNA during
            transcription. This enzyme catalyzes the transient breaking and
            rejoining of a single strand of DNA which allows the strands to
            pass through one another, thus relaxing the supercoils and altering
            the topology of DNA. The enzyme interacts with DNA helicase SGS1
            and plays a role in DNA recombination, cellular aging and
            maintenance of genome stability. Low expression of this gene may be
            related to higher survival rates in breast cancer patients. This
            gene has a pseudogene on chromosome 22. Alternate splicing results
            in multiple transcript variants. Additional alternatively spliced
            transcript variants of this gene have been described, but their
            full-length nature is not known. [provided by RefSeq, Aug 2013].
            
            Transcript Variant: This variant (4) differs in the 5' UTR compared
            to variant 1. Variants 1-5 encode the same protein (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.164863.1,
                                           SRR1803613.14211.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2158188 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 DA025312.1         57-59
            4-494               DA124773.1         1-491
            495-1716            AK290997.1         461-1682
            1717-1717           AC245452.3         107218-107218       c
            1718-2863           AK290997.1         1684-2829
            2864-2870           BQ270362.1         372-378
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q11.22"
     gene            1..2870
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /note="DNA topoisomerase III beta"
                     /db_xref="GeneID:8940"
                     /db_xref="HGNC:HGNC:11993"
                     /db_xref="MIM:603582"
     exon            1..58
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            59..94
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            95..262
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     CDS             193..2781
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /EC_number="5.6.2.1"
                     /note="isoform 1 is encoded by transcript variant 4;
                     topoisomerase III beta; DNA topoisomerase 3-beta-1;
                     topoisomerase (DNA) III beta; DNA topoisomerase III
                     beta-1"
                     /codon_start=1
                     /product="DNA topoisomerase 3-beta-1 isoform 1"
                     /protein_id="NP_001336774.1"
                     /db_xref="CCDS:CCDS13797.1"
                     /db_xref="GeneID:8940"
                     /db_xref="HGNC:HGNC:11993"
                     /db_xref="MIM:603582"
                     /translation="MKTVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGT
                     FAGQPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFL
                     QVEGRGCDYIVLWLDCDKEGENICFEVLDAVLPVMNKAHGGEKTVFRARFSSITDTDI
                     CNAMACLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQ
                     TPTLGFCVERHDKIQSFKPETYWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNM
                     TKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYI
                     SYPRTETTHYPENFDLKGSLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITP
                     MKSATEAELGGDAWRLYEYITRHFIATVSHDCKYLQSTISFRIGPELFTCSGKTVLSP
                     GFTEVMPWQSVPLEESLPTCQRGDAFPVGEVKMLEKQTNPPDYLTEAELITLMEKHGI
                     GTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEK
                     QLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRC
                     GKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRG
                     KSYPLCPYCYNHPPFRDMKKGMGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTS
                     GPKWKVACNKCNVVAHCFENAHRVRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHM
                     GCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRGRARRPPGKPNPRRPKDKMSA
                     LAAYFV"
     exon            263..394
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            395..501
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            502..576
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            577..773
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            774..930
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            931..1044
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1135
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1290
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..1396
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1543
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1544..1717
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1718..1846
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1847..1996
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1997..2097
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            2098..2299
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            2300..2870
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gttgtcggta gccgccccgg gaacaaggac cggagctgga tccgcggtgc ggctgtggct
       61 ctttagggtg agatctgctg taccagtctc acaggagtca gcattggtaa tagtgctgtg
      121 ctgcggaaga gtggagtcta aactttttca ttgccagtgc cccgaaactg gagtgtgaag
      181 gaccgagaca aaatgaagac tgtgctcatg gttgctgaaa agccgtcctt ggcacagtca
      241 attgccaaaa tcctctctag agggagcctg tcctcacaca aagggctgaa cggggcctgc
      301 tcagtccacg agtacactgg gacctttgct ggccagccag tgcgcttcaa gatgacgtct
      361 gtctgtggtc acgtgatgac cctggatttc ctgggaaaat acaacaaatg ggacaaagtg
      421 gaccccgcag aactgttcag ccaagctccc acggagaaga aagaagctaa ccccaagctg
      481 aacatggtga agttcctgca ggtggagggc agaggctgcg actacatcgt gctgtggctg
      541 gactgcgaca aggaggggga gaacatctgc tttgaggttc ttgatgctgt tctgcccgtc
      601 atgaacaagg cccatggtgg cgagaagacc gtgttccggg ccaggtttag ctccatcacg
      661 gacacagaca tctgtaatgc catggcctgc ctaggcgagc ctgaccacaa cgaggcgctc
      721 tcagtggatg ctcgccagga gctggacctg cgaatcggct gtgcattcac caggtttcag
      781 actaaatatt tccaggggaa atacggtgat ttagacagct ctctcatctc ctttgggccg
      841 tgtcagactc caaccctggg attctgtgtg gagagacatg ataaaatcca gtccttcaaa
      901 ccagagacct actgggtgct gcaggccaag gttaacactg acaaagacag atctctcctt
      961 ttggactggg accgagtaag agtgtttgac cgggagatcg cacagatgtt tttaaacatg
     1021 acaaagctgg agaaggaagc ccaggtggag gccacaagca ggaaagaaaa ggccaagcag
     1081 aggcccctgg ccctgaacac tgtggagatg ctgcgtgtgg ccagctcttc tctgggcatg
     1141 gggccgcagc acgccatgca gacggctgag cggctctaca cgcaaggcta catcagctac
     1201 ccacggacag agaccaccca ctaccctgag aactttgacc tgaagggctc tctgcggcag
     1261 caggccaacc acccctactg ggccgacacg gtgaagcggt tgttagcaga aggtatcaac
     1321 cgcccgcgga aaggccatga cgccggcgac catcccccca tcacccccat gaagtctgcc
     1381 acagaggccg aattaggggg tgacgcgtgg cggctctatg agtacatcac cagacacttc
     1441 atcgccacgg tcagccatga ctgcaagtac ctgcagagca ccatctcctt cagaattggg
     1501 cccgagctct tcacctgctc cgggaagacc gtcctctcac caggcttcac ggaggtcatg
     1561 ccctggcaga gcgtgcccct ggaggagagc ctgcccactt gccagcgggg tgatgccttc
     1621 cctgtgggcg aggtgaagat gctggagaag cagacgaacc cacccgacta cctgacggag
     1681 gccgagctca tcacgctcat ggagaagcat ggcatcggca cggatgccag catccctgtg
     1741 catatcaaca acatctgcca gcgcaactat gtcacggtgg agagcgggcg ccggctcaag
     1801 cccaccaacc tcggcatcgt cctggtgcac ggctactata agattgatgc agagctggtg
     1861 ctccccacca tccgcagtgc agtggagaag cagctgaacc tgatcgccca gggcaaggcc
     1921 gactaccgcc aggtcctggg ccacaccctg gacgtgttca agaggaagtt ccactacttt
     1981 gtcgactcca ttgctggcat ggatgagttg atggaggtgt ctttctcgcc cctggcggcc
     2041 acaggcaagc ccctctcacg ctgtgggaag tgccaccgct tcatgaagta catccaggcc
     2101 aagccaagcc gcctgcactg ctcccactgc gatgagacct acacgctccc ccagaacggc
     2161 accatcaagc tctacaagga gctccgctgc cctctggatg acttcgagct ggtcctgtgg
     2221 tcatcaggct ctcggggcaa gagctacccg ctgtgcccct actgctacaa ccacccaccc
     2281 ttccgagaca tgaagaaagg catgggctgc aacgagtgta cgcacccctc ctgccagcac
     2341 tcgctgagca tgctgggcat cggccagtgc gtggaatgtg agagcggggt gctggtgctg
     2401 gaccccacct cgggccccaa gtggaaggtg gcctgcaaca agtgcaacgt ggtagcgcac
     2461 tgcttcgaga acgcccaccg cgtgcgggtg tccgccgaca cctgcagtgt ctgtgaggcc
     2521 gccttgcttg atgtggactt caacaaggcc aagtccccac tcccgggcga tgagacgcag
     2581 cacatgggct gcgtcttttg tgaccccgtc ttccaggagc tggtggagct gaagcatgcg
     2641 gcctcctgcc accccatgca ccgcggtgga ccagggagaa ggcagggtcg agggcggggc
     2701 cgggccagga ggccccctgg gaagcccaac cccagacggc ccaaggacaa gatgtcagcc
     2761 ctggccgcct actttgtatg atgaccctgt cctccctcac ccaggctgca gtgccatgca
     2821 gacacctcat ggcacttcaa actattaaaa tgcattttat cttatccaga
//
