LOCUS       NM_001282112            2834 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens DNA topoisomerase III beta (TOP3B), transcript variant
            2, mRNA.
ACCESSION   NM_001282112 XM_005261810
VERSION     NM_001282112.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Yang,X., Saha,S., Yang,W., Neuman,K.C. and Pommier,Y.
  TITLE     Structural and biochemical basis for DNA and RNA catalysis by human
            Topoisomerase 3beta
  JOURNAL   Nat Commun 13 (1), 4656 (2022)
   PUBMED   35945419
  REMARK    GeneRIF: Structural and biochemical basis for DNA and RNA catalysis
            by human Topoisomerase 3beta.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Su,S., Xue,Y., Sharov,A., Zhang,Y., Lee,S.K., Martindale,J.L.,
            Li,W., Ku,W.L., Zhao,K., De,S., Shen,W., Sen,P., Gorospe,M., Xu,D.
            and Wang,W.
  TITLE     A dual-activity topoisomerase complex regulates mRNA translation
            and turnover
  JOURNAL   Nucleic Acids Res 50 (12), 7013-7033 (2022)
   PUBMED   35748872
  REMARK    GeneRIF: A dual-activity topoisomerase complex regulates mRNA
            translation and turnover.
REFERENCE   3  (bases 1 to 2834)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2834)
  AUTHORS   Saha,S., Sun,Y., Huang,S.N., Baechler,S.A., Pongor,L.S., Agama,K.,
            Jo,U., Zhang,H., Tse-Dinh,Y.C. and Pommier,Y.
  TITLE     DNA and RNA Cleavage Complexes and Repair Pathway for TOP3B RNA-
            and DNA-Protein Crosslinks
  JOURNAL   Cell Rep 33 (13), 108569 (2020)
   PUBMED   33378676
  REMARK    GeneRIF: DNA and RNA Cleavage Complexes and Repair Pathway for
            TOP3B RNA- and DNA-Protein Crosslinks.
REFERENCE   5  (bases 1 to 2834)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   6  (bases 1 to 2834)
  AUTHORS   Oliveira-Costa,J.P., Zanetti,J., Oliveira,L.R., Soares,F.A.,
            Ramalho,L.Z., Silva Ramalho,F., Garcia,S.B. and Ribeiro-Silva,A.
  TITLE     Significance of topoisomerase IIIbeta expression in breast ductal
            carcinomas: strong associations with disease-specific survival and
            metastasis
  JOURNAL   Hum Pathol 41 (11), 1624-1630 (2010)
   PUBMED   20950730
  REMARK    GeneRIF: Topoisomerase IIIbeta expression can be a useful marker in
            assessing the prognosis of patients with breast cancer and is an
            independent predictor of survival.
            Erratum:[Hum Pathol. 2012 Apr;43(4):618]
REFERENCE   7  (bases 1 to 2834)
  AUTHORS   Cho,Y.H., Park,J.Y., Han,S.Y. and Chung,I.K.
  TITLE     Identification of the functional elements in the promoter region of
            human DNA topoisomerase IIIbeta gene
  JOURNAL   Biochim Biophys Acta 1679 (3), 272-278 (2004)
   PUBMED   15358519
  REMARK    GeneRIF: the positive regulatory region including the 5'GGAACC3'
            core element is essential for efficient expression of the DNA
            topoisomerase III beta gene
REFERENCE   8  (bases 1 to 2834)
  AUTHORS   Kobayashi,M. and Hanai,R.
  TITLE     M phase-specific association of human topoisomerase IIIbeta with
            chromosomes
  JOURNAL   Biochem Biophys Res Commun 287 (1), 282-287 (2001)
   PUBMED   11549288
  REMARK    GeneRIF: Two isoforms of human DNA topoisomerase IIIbeta were
            expressed in HeLa cells. Isoform 1 was localized to the nucleus and
            associated with chromosomes during metaphase and anaphase. Isoform
            2 was found in the cytoplasm.
REFERENCE   9  (bases 1 to 2834)
  AUTHORS   Shimamoto,A., Nishikawa,K., Kitao,S. and Furuichi,Y.
  TITLE     Human RecQ5beta, a large isomer of RecQ5 DNA helicase, localizes in
            the nucleoplasm and interacts with topoisomerases 3alpha and 3beta
  JOURNAL   Nucleic Acids Res 28 (7), 1647-1655 (2000)
   PUBMED   10710432
REFERENCE   10 (bases 1 to 2834)
  AUTHORS   Ng,S.W., Liu,Y., Hasselblatt,K.T., Mok,S.C. and Berkowitz,R.S.
  TITLE     A new human topoisomerase III that interacts with SGS1 protein
  JOURNAL   Nucleic Acids Res 27 (4), 993-1000 (1999)
   PUBMED   9927731
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA025312.1, AF053082.1,
            AC245452.3 and BQ270362.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001282112.1.
            
            Summary: This gene encodes a DNA topoisomerase, an enzyme that
            controls and alters the topologic states of DNA during
            transcription. This enzyme catalyzes the transient breaking and
            rejoining of a single strand of DNA which allows the strands to
            pass through one another, thus relaxing the supercoils and altering
            the topology of DNA. The enzyme interacts with DNA helicase SGS1
            and plays a role in DNA recombination, cellular aging and
            maintenance of genome stability. Low expression of this gene may be
            related to higher survival rates in breast cancer patients. This
            gene has a pseudogene on chromosome 22. Alternate splicing results
            in multiple transcript variants. Additional alternatively spliced
            transcript variants of this gene have been described, but their
            full-length nature is not known. [provided by RefSeq, Aug 2013].
            
            Transcript Variant: This variant (2) contains an alternate 5'
            terminal exon compared to variant 1. Variants 1-5 encode the same
            protein (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.7267.1,
                                           SRR1660809.158208.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2158188, SAMEA2158569
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000357179.10/ ENSP00000349705.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-43                DA025312.1         57-99
            44-155              AF053082.1         1-112
            156-156             AC245452.3         120499-120499       c
            157-2828            AF053082.1         114-2785
            2829-2834           BQ270362.1         373-378
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q11.22"
     gene            1..2834
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /note="DNA topoisomerase III beta"
                     /db_xref="GeneID:8940"
                     /db_xref="HGNC:HGNC:11993"
                     /db_xref="MIM:603582"
     exon            1..58
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            59..226
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     CDS             157..2745
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /EC_number="5.6.2.1"
                     /note="isoform 1 is encoded by transcript variant 2;
                     topoisomerase III beta; DNA topoisomerase 3-beta-1;
                     topoisomerase (DNA) III beta; DNA topoisomerase III
                     beta-1"
                     /codon_start=1
                     /product="DNA topoisomerase 3-beta-1 isoform 1"
                     /protein_id="NP_001269041.1"
                     /db_xref="CCDS:CCDS13797.1"
                     /db_xref="GeneID:8940"
                     /db_xref="HGNC:HGNC:11993"
                     /db_xref="MIM:603582"
                     /translation="MKTVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGT
                     FAGQPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFL
                     QVEGRGCDYIVLWLDCDKEGENICFEVLDAVLPVMNKAHGGEKTVFRARFSSITDTDI
                     CNAMACLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQ
                     TPTLGFCVERHDKIQSFKPETYWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNM
                     TKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYI
                     SYPRTETTHYPENFDLKGSLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITP
                     MKSATEAELGGDAWRLYEYITRHFIATVSHDCKYLQSTISFRIGPELFTCSGKTVLSP
                     GFTEVMPWQSVPLEESLPTCQRGDAFPVGEVKMLEKQTNPPDYLTEAELITLMEKHGI
                     GTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEK
                     QLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRC
                     GKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRG
                     KSYPLCPYCYNHPPFRDMKKGMGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTS
                     GPKWKVACNKCNVVAHCFENAHRVRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHM
                     GCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRGRARRPPGKPNPRRPKDKMSA
                     LAAYFV"
     exon            227..358
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            359..465
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            466..540
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            541..737
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            738..894
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            895..1008
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1099
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1100..1254
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1255..1360
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1507
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1681
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1682..1810
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1811..1960
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            1961..2061
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            2062..2263
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
     exon            2264..2834
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gttgtcggta gccgccccgg gaacaaggac cggagctgga tccgcggtgc ggctgtggga
       61 gtcagcattg gtaatagtgc tgtgctgcgg aagagtggag tctaaacttt ttcattgcca
      121 gtgccccgaa actggagtgt gaaggaccga gacaaaatga agactgtgct catggttgct
      181 gaaaagccgt ccttggcaca gtcaattgcc aaaatcctct ctagagggag cctgtcctca
      241 cacaaagggc tgaacggggc ctgctcagtc cacgagtaca ctgggacctt tgctggccag
      301 ccagtgcgct tcaagatgac gtctgtctgt ggtcacgtga tgaccctgga tttcctggga
      361 aaatacaaca aatgggacaa agtggacccc gcagaactgt tcagccaagc tcccacggag
      421 aagaaagaag ctaaccccaa gctgaacatg gtgaagttcc tgcaggtgga gggcagaggc
      481 tgcgactaca tcgtgctgtg gctggactgc gacaaggagg gggagaacat ctgctttgag
      541 gttcttgatg ctgttctgcc cgtcatgaac aaggcccatg gtggcgagaa gaccgtgttc
      601 cgggccaggt ttagctccat cacggacaca gacatctgta atgccatggc ctgcctaggc
      661 gagcctgacc acaacgaggc gctctcagtg gatgctcgcc aggagctgga cctgcgaatc
      721 ggctgtgcat tcaccaggtt tcagactaaa tatttccagg ggaaatacgg tgatttagac
      781 agctctctca tctcctttgg gccgtgtcag actccaaccc tgggattctg tgtggagaga
      841 catgataaaa tccagtcctt caaaccagag acctactggg tgctgcaggc caaggttaac
      901 actgacaaag acagatctct ccttttggac tgggaccgag taagagtgtt tgaccgggag
      961 atcgcacaga tgtttttaaa catgacaaag ctggagaagg aagcccaggt ggaggccaca
     1021 agcaggaaag aaaaggccaa gcagaggccc ctggccctga acactgtgga gatgctgcgt
     1081 gtggccagct cttctctggg catggggccg cagcacgcca tgcagacggc tgagcggctc
     1141 tacacgcaag gctacatcag ctacccacgg acagagacca cccactaccc tgagaacttt
     1201 gacctgaagg gctctctgcg gcagcaggcc aaccacccct actgggccga cacggtgaag
     1261 cggttgttag cagaaggtat caaccgcccg cggaaaggcc atgacgccgg cgaccatccc
     1321 cccatcaccc ccatgaagtc tgccacagag gccgaattag ggggtgacgc gtggcggctc
     1381 tatgagtaca tcaccagaca cttcatcgcc acggtcagcc atgactgcaa gtacctgcag
     1441 agcaccatct ccttcagaat tgggcccgag ctcttcacct gctccgggaa gaccgtcctc
     1501 tcaccaggct tcacggaggt catgccctgg cagagcgtgc ccctggagga gagcctgccc
     1561 acttgccagc ggggtgatgc cttccctgtg ggcgaggtga agatgctgga gaagcagacg
     1621 aacccacccg actacctgac ggaggccgag ctcatcacgc tcatggagaa gcatggcatc
     1681 ggcacggatg ccagcatccc tgtgcatatc aacaacatct gccagcgcaa ctatgtcacg
     1741 gtggagagcg ggcgccggct caagcccacc aacctcggca tcgtcctggt gcacggctac
     1801 tataagattg atgcagagct ggtgctcccc accatccgca gtgcagtgga gaagcagctg
     1861 aacctgatcg cccagggcaa ggccgactac cgccaggtcc tgggccacac cctggacgtg
     1921 ttcaagagga agttccacta ctttgtcgac tccattgctg gcatggatga gttgatggag
     1981 gtgtctttct cgcccctggc ggccacaggc aagcccctct cacgctgtgg gaagtgccac
     2041 cgcttcatga agtacatcca ggccaagcca agccgcctgc actgctccca ctgcgatgag
     2101 acctacacgc tcccccagaa cggcaccatc aagctctaca aggagctccg ctgccctctg
     2161 gatgacttcg agctggtcct gtggtcatca ggctctcggg gcaagagcta cccgctgtgc
     2221 ccctactgct acaaccaccc acccttccga gacatgaaga aaggcatggg ctgcaacgag
     2281 tgtacgcacc cctcctgcca gcactcgctg agcatgctgg gcatcggcca gtgcgtggaa
     2341 tgtgagagcg gggtgctggt gctggacccc acctcgggcc ccaagtggaa ggtggcctgc
     2401 aacaagtgca acgtggtagc gcactgcttc gagaacgccc accgcgtgcg ggtgtccgcc
     2461 gacacctgca gtgtctgtga ggccgccttg cttgatgtgg acttcaacaa ggccaagtcc
     2521 ccactcccgg gcgatgagac gcagcacatg ggctgcgtct tttgtgaccc cgtcttccag
     2581 gagctggtgg agctgaagca tgcggcctcc tgccacccca tgcaccgcgg tggaccaggg
     2641 agaaggcagg gtcgagggcg gggccgggcc aggaggcccc ctgggaagcc caaccccaga
     2701 cggcccaagg acaagatgtc agccctggcc gcctactttg tatgatgacc ctgtcctccc
     2761 tcacccaggc tgcagtgcca tgcagacacc tcatggcact tcaaactatt aaaatgcatt
     2821 ttatcttatc caga
//
