LOCUS       NP_001269041             862 aa            linear   PRI 28-APR-2025
DEFINITION  DNA topoisomerase 3-beta-1 isoform 1 [Homo sapiens].
ACCESSION   NP_001269041 XP_005261867
VERSION     NP_001269041.1
DBSOURCE    REFSEQ: accession NM_001282112.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 862)
  AUTHORS   Yang,X., Saha,S., Yang,W., Neuman,K.C. and Pommier,Y.
  TITLE     Structural and biochemical basis for DNA and RNA catalysis by human
            Topoisomerase 3beta
  JOURNAL   Nat Commun 13 (1), 4656 (2022)
   PUBMED   35945419
  REMARK    GeneRIF: Structural and biochemical basis for DNA and RNA catalysis
            by human Topoisomerase 3beta.
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 862)
  AUTHORS   Su,S., Xue,Y., Sharov,A., Zhang,Y., Lee,S.K., Martindale,J.L.,
            Li,W., Ku,W.L., Zhao,K., De,S., Shen,W., Sen,P., Gorospe,M., Xu,D.
            and Wang,W.
  TITLE     A dual-activity topoisomerase complex regulates mRNA translation
            and turnover
  JOURNAL   Nucleic Acids Res 50 (12), 7013-7033 (2022)
   PUBMED   35748872
  REMARK    GeneRIF: A dual-activity topoisomerase complex regulates mRNA
            translation and turnover.
REFERENCE   3  (residues 1 to 862)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (residues 1 to 862)
  AUTHORS   Saha,S., Sun,Y., Huang,S.N., Baechler,S.A., Pongor,L.S., Agama,K.,
            Jo,U., Zhang,H., Tse-Dinh,Y.C. and Pommier,Y.
  TITLE     DNA and RNA Cleavage Complexes and Repair Pathway for TOP3B RNA-
            and DNA-Protein Crosslinks
  JOURNAL   Cell Rep 33 (13), 108569 (2020)
   PUBMED   33378676
  REMARK    GeneRIF: DNA and RNA Cleavage Complexes and Repair Pathway for
            TOP3B RNA- and DNA-Protein Crosslinks.
REFERENCE   5  (residues 1 to 862)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   6  (residues 1 to 862)
  AUTHORS   Oliveira-Costa,J.P., Zanetti,J., Oliveira,L.R., Soares,F.A.,
            Ramalho,L.Z., Silva Ramalho,F., Garcia,S.B. and Ribeiro-Silva,A.
  TITLE     Significance of topoisomerase IIIbeta expression in breast ductal
            carcinomas: strong associations with disease-specific survival and
            metastasis
  JOURNAL   Hum Pathol 41 (11), 1624-1630 (2010)
   PUBMED   20950730
  REMARK    GeneRIF: Topoisomerase IIIbeta expression can be a useful marker in
            assessing the prognosis of patients with breast cancer and is an
            independent predictor of survival.
            Erratum:[Hum Pathol. 2012 Apr;43(4):618]
REFERENCE   7  (residues 1 to 862)
  AUTHORS   Cho,Y.H., Park,J.Y., Han,S.Y. and Chung,I.K.
  TITLE     Identification of the functional elements in the promoter region of
            human DNA topoisomerase IIIbeta gene
  JOURNAL   Biochim Biophys Acta 1679 (3), 272-278 (2004)
   PUBMED   15358519
  REMARK    GeneRIF: the positive regulatory region including the 5'GGAACC3'
            core element is essential for efficient expression of the DNA
            topoisomerase III beta gene
REFERENCE   8  (residues 1 to 862)
  AUTHORS   Kobayashi,M. and Hanai,R.
  TITLE     M phase-specific association of human topoisomerase IIIbeta with
            chromosomes
  JOURNAL   Biochem Biophys Res Commun 287 (1), 282-287 (2001)
   PUBMED   11549288
  REMARK    GeneRIF: Two isoforms of human DNA topoisomerase IIIbeta were
            expressed in HeLa cells. Isoform 1 was localized to the nucleus and
            associated with chromosomes during metaphase and anaphase. Isoform
            2 was found in the cytoplasm.
REFERENCE   9  (residues 1 to 862)
  AUTHORS   Shimamoto,A., Nishikawa,K., Kitao,S. and Furuichi,Y.
  TITLE     Human RecQ5beta, a large isomer of RecQ5 DNA helicase, localizes in
            the nucleoplasm and interacts with topoisomerases 3alpha and 3beta
  JOURNAL   Nucleic Acids Res 28 (7), 1647-1655 (2000)
   PUBMED   10710432
REFERENCE   10 (residues 1 to 862)
  AUTHORS   Ng,S.W., Liu,Y., Hasselblatt,K.T., Mok,S.C. and Berkowitz,R.S.
  TITLE     A new human topoisomerase III that interacts with SGS1 protein
  JOURNAL   Nucleic Acids Res 27 (4), 993-1000 (1999)
   PUBMED   9927731
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA025312.1, AF053082.1,
            AC245452.3 and BQ270362.1.
            
            On Aug 31, 2013 this sequence version replaced XP_005261867.1.
            
            Summary: This gene encodes a DNA topoisomerase, an enzyme that
            controls and alters the topologic states of DNA during
            transcription. This enzyme catalyzes the transient breaking and
            rejoining of a single strand of DNA which allows the strands to
            pass through one another, thus relaxing the supercoils and altering
            the topology of DNA. The enzyme interacts with DNA helicase SGS1
            and plays a role in DNA recombination, cellular aging and
            maintenance of genome stability. Low expression of this gene may be
            related to higher survival rates in breast cancer patients. This
            gene has a pseudogene on chromosome 22. Alternate splicing results
            in multiple transcript variants. Additional alternatively spliced
            transcript variants of this gene have been described, but their
            full-length nature is not known. [provided by RefSeq, Aug 2013].
            
            Transcript Variant: This variant (2) contains an alternate 5'
            terminal exon compared to variant 1. Variants 1-5 encode the same
            protein (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.7267.1,
                                           SRR1660809.158208.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2158188, SAMEA2158569
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000357179.10/ ENSP00000349705.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q11.22"
     Protein         1..862
                     /product="DNA topoisomerase 3-beta-1 isoform 1"
                     /EC_number="5.6.2.1"
                     /note="topoisomerase III beta; DNA topoisomerase 3-beta-1;
                     topoisomerase (DNA) III beta; DNA topoisomerase III
                     beta-1"
                     /calculated_mol_wt=96531
     Region          3..167
                     /region_name="TOPRIM_TopoIA_TopoIII"
                     /note="The topoisomerase-primase (TORPIM) domain found in
                     members of the type IA family of DNA topoisomerases (Topo
                     IA) similar to topoisomerase III. Type IA DNA
                     topoisomerases remove (relax) negative supercoils in the
                     DNA by: cleaving one strand of the DNA...; cd03362"
                     /db_xref="CDD:173782"
     Site            order(9..10,13,117,119,121)
                     /site_type="active"
                     /db_xref="CDD:173782"
     Site            order(16,118,120,127..128,130..131)
                     /site_type="other"
                     /note="putative interdomain interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:173782"
     Site            order(117,119)
                     /site_type="other"
                     /note="putative metal-binding site [ion binding]"
                     /db_xref="CDD:173782"
     Site            120
                     /site_type="other"
                     /note="putative nucleotide binding site [chemical
                     binding]"
                     /db_xref="CDD:173782"
     Region          172..593
                     /region_name="TOP1Ac"
                     /note="DNA Topoisomerase, subtype IA; DNA-binding,
                     ATP-binding and catalytic domain of bacterial DNA
                     topoisomerases I and III, and eukaryotic DNA topoisomerase
                     III and eubacterial and archael reverse gyrases.
                     Topoisomerases clevage single or double stranded DNA...;
                     cd00186"
                     /db_xref="CDD:238110"
     Site            order(172..199,212..232)
                     /site_type="active"
                     /note="domain I [active]"
                     /db_xref="CDD:238110"
     Site            order(181..182,185,189,193,326,330,338,512..513,516..517,
                     519,523..524)
                     /site_type="DNA binding"
                     /note="DNA binding groove [nucleotide binding]"
                     /db_xref="CDD:238110"
     Site            order(223,227,549)
                     /site_type="other"
                     /note="phosphate binding site [ion binding]"
                     /db_xref="CDD:238110"
     Site            order(237..244,282..296,422..436,438..456,479..489)
                     /site_type="active"
                     /note="domain II [active]"
                     /db_xref="CDD:238110"
     Site            order(297..303,305..360,386..394,401..421)
                     /site_type="active"
                     /note="domain III [active]"
                     /db_xref="CDD:238110"
     Site            order(308,312..314,387,407,410,414,510,512,535)
                     /site_type="other"
                     /note="nucleotide binding site [chemical binding]"
                     /db_xref="CDD:238110"
     Site            order(336,338,387)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:238110"
     Site            order(490..530,535..549,555..593)
                     /site_type="active"
                     /note="domain IV [active]"
                     /db_xref="CDD:238110"
     Region          821..854
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O95985.1)"
     CDS             1..862
                     /gene="TOP3B"
                     /gene_synonym="TOP3B1"
                     /coded_by="NM_001282112.2:157..2745"
                     /note="isoform 1 is encoded by transcript variant 2"
                     /db_xref="CCDS:CCDS13797.1"
                     /db_xref="GeneID:8940"
                     /db_xref="HGNC:HGNC:11993"
                     /db_xref="MIM:603582"
ORIGIN      
        1 mktvlmvaek pslaqsiaki lsrgslsshk glngacsvhe ytgtfagqpv rfkmtsvcgh
       61 vmtldflgky nkwdkvdpae lfsqaptekk eanpklnmvk flqvegrgcd yivlwldcdk
      121 egenicfevl davlpvmnka hggektvfra rfssitdtdi cnamaclgep dhnealsvda
      181 rqeldlrigc aftrfqtkyf qgkygdldss lisfgpcqtp tlgfcverhd kiqsfkpety
      241 wvlqakvntd kdrsllldwd rvrvfdreia qmflnmtkle keaqveatsr kekakqrpla
      301 lntvemlrva ssslgmgpqh amqtaerlyt qgyisyprte tthypenfdl kgslrqqanh
      361 pywadtvkrl laeginrprk ghdagdhppi tpmksateae lggdawrlye yitrhfiatv
      421 shdckylqst isfrigpelf tcsgktvlsp gftevmpwqs vpleeslptc qrgdafpvge
      481 vkmlekqtnp pdylteaeli tlmekhgigt dasipvhinn icqrnyvtve sgrrlkptnl
      541 givlvhgyyk idaelvlpti rsavekqlnl iaqgkadyrq vlghtldvfk rkfhyfvdsi
      601 agmdelmevs fsplaatgkp lsrcgkchrf mkyiqakpsr lhcshcdety tlpqngtikl
      661 ykelrcpldd felvlwssgs rgksyplcpy cynhppfrdm kkgmgcnect hpscqhslsm
      721 lgigqcvece sgvlvldpts gpkwkvacnk cnvvahcfen ahrvrvsadt csvceaalld
      781 vdfnkakspl pgdetqhmgc vfcdpvfqel velkhaasch pmhrggpgrr qgrgrgrarr
      841 ppgkpnprrp kdkmsalaay fv
//
