LOCUS       NM_004618               6596 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript
            variant 1, mRNA.
ACCESSION   NM_004618
VERSION     NM_004618.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Erdinc,D., Rodriguez-Luis,A., Fassad,M.R., Mackenzie,S.,
            Watson,C.M., Valenzuela,S., Xie,X., Menger,K.E., Sergeant,K.,
            Craig,K., Hopton,S., Falkous,G., Poulton,J., Garcia-Moreno,H.,
            Giunti,P., de Moura Aschoff,C.A., Morales Saute,J.A., Kirby,A.J.,
            Toro,C., Wolfe,L., Novacic,D., Greenbaum,L., Eliyahu,A., Barel,O.,
            Anikster,Y., McFarland,R., Gorman,G.S., Schaefer,A.M.,
            Gustafsson,C.M., Taylor,R.W., Falkenberg,M. and Nicholls,T.J.
  CONSRTM   Genomics England Research Consortium
  TITLE     Pathological variants in TOP3A cause distinct disorders of
            mitochondrial and nuclear genome stability
  JOURNAL   EMBO Mol Med 15 (5), e16775 (2023)
   PUBMED   37013609
  REMARK    GeneRIF: Pathological variants in TOP3A cause distinct disorders of
            mitochondrial and nuclear genome stability.
REFERENCE   2  (bases 1 to 6596)
  AUTHORS   Soniat,M.M., Nguyen,G., Kuo,H.C. and Finkelstein,I.J.
  TITLE     The MRN complex and topoisomerase IIIa-RMI1/2 synchronize DNA
            resection motor proteins
  JOURNAL   J Biol Chem 299 (2), 102802 (2023)
   PUBMED   36529288
  REMARK    GeneRIF: The MRN complex and topoisomerase IIIa-RMI1/2 synchronize
            DNA resection motor proteins.
REFERENCE   3  (bases 1 to 6596)
  AUTHORS   Menger,K.E., Chapman,J., Diaz-Maldonado,H., Khazeem,M.M., Deen,D.,
            Erdinc,D., Casement,J.W., Di Leo,V., Pyle,A., Rodriguez-Luis,A.,
            Cowell,I.G., Falkenberg,M., Austin,C.A. and Nicholls,T.J.
  TITLE     Two type I topoisomerases maintain DNA topology in human
            mitochondria
  JOURNAL   Nucleic Acids Res 50 (19), 11154-11174 (2022)
   PUBMED   36215039
  REMARK    GeneRIF: Two type I topoisomerases maintain DNA topology in human
            mitochondria.
REFERENCE   4  (bases 1 to 6596)
  AUTHORS   de Nonneville,A., Salas,S., Bertucci,F., Sobinoff,A.P.,
            Adelaide,J., Guille,A., Finetti,P., Noble,J.R., Churikov,D.,
            Chaffanet,M., Lavit,E., Pickett,H.A., Bouvier,C., Birnbaum,D.,
            Reddel,R.R. and Geli,V.
  TITLE     TOP3A amplification and ATRX inactivation are mutually exclusive
            events in pediatric osteosarcomas using ALT
  JOURNAL   EMBO Mol Med 14 (10), e15859 (2022)
   PUBMED   35920001
  REMARK    GeneRIF: TOP3A amplification and ATRX inactivation are mutually
            exclusive events in pediatric osteosarcomas using ALT.
REFERENCE   5  (bases 1 to 6596)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 6596)
  AUTHORS   Shimamoto,A., Nishikawa,K., Kitao,S. and Furuichi,Y.
  TITLE     Human RecQ5beta, a large isomer of RecQ5 DNA helicase, localizes in
            the nucleoplasm and interacts with topoisomerases 3alpha and 3beta
  JOURNAL   Nucleic Acids Res 28 (7), 1647-1655 (2000)
   PUBMED   10710432
REFERENCE   7  (bases 1 to 6596)
  AUTHORS   Goulaouic,H., Roulon,T., Flamand,O., Grondard,L., Lavelle,F. and
            Riou,J.F.
  TITLE     Purification and characterization of human DNA topoisomerase
            IIIalpha
  JOURNAL   Nucleic Acids Res 27 (12), 2443-2450 (1999)
   PUBMED   10352172
REFERENCE   8  (bases 1 to 6596)
  AUTHORS   Kim,J.C., Yoon,J.B., Koo,H.S. and Chung,I.K.
  TITLE     Cloning and characterization of the 5'-flanking region for the
            human topoisomerase III gene
  JOURNAL   J Biol Chem 273 (40), 26130-26137 (1998)
   PUBMED   9748294
REFERENCE   9  (bases 1 to 6596)
  AUTHORS   Fritz,E., Elsea,S.H., Patel,P.I. and Meyn,M.S.
  TITLE     Overexpression of a truncated human topoisomerase III partially
            corrects multiple aspects of the ataxia-telangiectasia phenotype
  JOURNAL   Proc Natl Acad Sci U S A 94 (9), 4538-4542 (1997)
   PUBMED   9114025
REFERENCE   10 (bases 1 to 6596)
  AUTHORS   Hanai,R., Caron,P.R. and Wang,J.C.
  TITLE     Human TOP3: a single-copy gene encoding DNA topoisomerase III
  JOURNAL   Proc Natl Acad Sci U S A 93 (8), 3653-3657 (1996)
   PUBMED   8622991
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BM462184.1, BC051748.1,
            AF086042.1, AC127537.8 and AW294300.1.
            
            On Nov 23, 2018 this sequence version replaced NM_004618.4.
            
            Summary: This gene encodes a DNA topoisomerase, an enzyme that
            controls and alters the topologic states of DNA during
            transcription. This enzyme catalyzes the transient breaking and
            rejoining of a single strand of DNA which allows the strands to
            pass through one another, thus reducing the number of supercoils
            and altering the topology of DNA. This enzyme forms a complex with
            BLM which functions in the regulation of recombination in somatic
            cells. Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Mar 2016].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.82081.1,
                                           SRR1803611.50754.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000321105.10/ ENSP00000321636.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                BM462184.1         100-119
            21-4065             BC051748.1         1-4045
            4066-4076           BC051748.1         4047-4057
            4077-4103           AF086042.1         518-544
            4104-6363           AC127537.8         45900-48159         c
            6364-6596           AW294300.1         15-247              c
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p11.2"
     gene            1..6596
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /note="DNA topoisomerase III alpha"
                     /db_xref="GeneID:7156"
                     /db_xref="HGNC:HGNC:11992"
                     /db_xref="MIM:601243"
     exon            1..396
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     CDS             217..3222
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /EC_number="5.6.2.1"
                     /note="isoform 1 is encoded by transcript variant 1; topo
                     III-alpha; DNA topoisomerase 3-alpha; zinc finger,
                     GRF-type containing 7; topoisomerase (DNA) III alpha"
                     /codon_start=1
                     /product="DNA topoisomerase 3-alpha isoform 1"
                     /protein_id="NP_004609.1"
                     /db_xref="CCDS:CCDS11194.1"
                     /db_xref="GeneID:7156"
                     /db_xref="HGNC:HGNC:11992"
                     /db_xref="MIM:601243"
                     /translation="MIFPVARYALRWLRRPEDRAFSRAAMEMALRGVRKVLCVAEKND
                     AAKGIADLLSNGRMRRREGLSKFNKIYEFDYHLYGQNVTMVMTSVSGHLLAHDFQMQF
                     RKWQSCNPLVLFEAEIEKYCPENFVDIKKTLERETRQCQALVIWTDCDREGENIGFEI
                     IHVCKAVKPNLQVLRARFSEITPHAVRTACENLTEPDQRVSDAVDVRQELDLRIGAAF
                     TRFQTLRLQRIFPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEIFHRIKVTHDH
                     KDGIVEFNWKRHRLFNHTACLVLYQLCVEDPMATVVEVRSKPKSKWRPQALDTVELEK
                     LASRKLRINAKETMRIAEKLYTQGYISYPRTETNIFPRDLNLTVLVEQQTPDPRWGAF
                     AQSILERGGPTPRNGNKSDQAHPPIHPTKYTNNLQGDEQRLYEFIVRHFLACCSQDAQ
                     GQETTVEIDIAQERFVAHGLMILARNYLDVYPYDHWSDKILPVYEQGSHFQPSTVEMV
                     DGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTPDKRFLPGHLGM
                     GLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKA
                     KKLDEALAQYFGNGTELAQQEDIYPAMPEPIRKCPQCNKDMVLKTKKNGGFYLSCMGF
                     PECRSAVWLPDSVLEASRDSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFVCCIGGC
                     DDTLREILDLRFSGGPPRASQPSGRLQANQSLNRMDNSQHPQPADSRQTGSSKALAQT
                     LPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGA
                     GGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQKDGPN
                     KGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLESEARSKRPRASSS
                     DMGSTAKKPRKCSLCHQPGHTRPFCPQNR"
     exon            397..456
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            457..530
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            531..606
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            607..715
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            716..859
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            860..1030
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            1031..1131
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1206
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            1207..1289
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            1290..1497
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            1498..1683
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            1684..1813
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            1814..1927
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            1928..2093
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            2094..2237
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            2238..2360
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            2361..3043
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            3044..6596
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3775..3780
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /note="hexamer: AATAAA"
     polyA_site      3794
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /note="major polyA site"
     polyA_site      6262
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
     regulatory      6576..6581
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /note="hexamer: ATTAAA"
     polyA_site      6596
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /note="major polyA site"
ORIGIN      
        1 ggaagttcca cgccaagata ctggattcgg tgctgaggcg gcggctgcgg cacgggaaag
       61 gctcagtgac tgaagctcca aaggccagca ggctggtggg gacgtgaccg aagcgaggct
      121 ctggttccct ttcggtgggc gccatttgag cctcatctct ggcttcccca ggatgcgccg
      181 gcagccgggg agcggctccg ggcgcgaggt ctgaggatga tctttcctgt cgcccgctac
      241 gcgctccggt ggctgcgacg gcccgaagac cgtgcctttt cccgcgccgc catggagatg
      301 gccctccgag gcgtgcggaa agtcctctgt gtggccgaaa aaaacgacgc ggccaagggg
      361 atcgccgacc tgctgtcaaa cggtcgcatg aggcggagag aaggactttc aaaattcaac
      421 aagatctatg aatttgatta tcatctgtat ggccagaatg ttaccatggt aatgacttca
      481 gtttctggac atttactggc tcatgatttc cagatgcagt ttcgaaaatg gcagagctgc
      541 aaccctcttg tcctctttga agcagaaatt gaaaagtact gcccagagaa ttttgtagac
      601 atcaagaaaa ctttggaacg agagactcgc cagtgccagg ctctggtgat ctggactgac
      661 tgtgatagag aaggcgaaaa catcgggttt gagattatcc acgtgtgtaa ggctgtaaag
      721 cccaatctgc aggtgttgcg agcccgattc tctgagatca caccccatgc cgtcaggaca
      781 gcttgtgaaa acctgaccga gcctgatcag agggtgagcg atgctgtgga tgtgaggcag
      841 gagctggacc tgaggattgg agctgccttt actaggttcc agaccctgcg gcttcagagg
      901 atttttcctg aggtgctggc agagcagctc atcagttacg gcagctgcca gttccccaca
      961 ctgggctttg tggtggagcg gttcaaagcc attcaggctt ttgtaccaga aatcttccac
     1021 agaattaaag taactcatga ccacaaagat ggtatcgtag aattcaactg gaaaaggcat
     1081 cgactcttta accacacggc ttgcctagtt ctctatcagt tgtgtgtgga ggatcccatg
     1141 gcaactgtgg tagaggtcag atctaagccc aagagcaagt ggcggcctca agccttggac
     1201 actgtggagc ttgagaagct ggcttctcga aagttgagaa taaatgctaa agaaaccatg
     1261 aggattgctg agaagctcta cactcaaggg tacatcagct atccccgaac agaaacaaac
     1321 atttttccca gagacttaaa cctgacggtg ttggtggaac agcagacccc cgatccacgc
     1381 tggggggcct ttgcccagag cattctagag cggggtggtc ccaccccacg caatgggaac
     1441 aagtctgacc aagctcaccc tcccattcac cccaccaaat acaccaacaa cttacaggga
     1501 gatgaacagc gactgtacga gtttattgtt cgccatttcc tggcttgctg ctcccaggat
     1561 gctcaggggc aggagaccac agtggagatc gacatcgctc aggaacgctt tgtggcccat
     1621 ggcctcatga ttctggcccg aaactatctg gatgtgtatc catatgatca ctggagtgac
     1681 aagatcctcc ctgtctatga gcaaggatcc cactttcagc ccagcaccgt ggagatggtg
     1741 gacggggaga ccagcccacc caagctgctc accgaggccg acctcattgc cctcatggag
     1801 aagcatggca ttggtacgga tgccactcat gcggagcaca tcgagaccat caaagcccgg
     1861 atgtacgtgg gcctcacccc agacaagcgg ttcctccctg ggcacctggg catgggactt
     1921 gtggaaggtt atgattccat gggctatgaa atgtctaagc ctgacctccg ggctgaactg
     1981 gaagctgatc tgaagctgat ctgtgatggc aaaaaggaca aatttgtggt tctaaggcag
     2041 caagtgcaga aatacaagca ggttttcatt gaagcggtgg ctaaagcaaa gaaattggac
     2101 gaggccttgg cccagtactt tgggaatggg acagagttgg cccagcaaga agatatctac
     2161 ccagccatgc cagagcccat caggaagtgc ccacagtgca acaaggacat ggtccttaag
     2221 accaagaaga atggcgggtt ctacctcagc tgcatgggtt tcccagagtg tcgctcagct
     2281 gtgtggcttc ctgactcggt gctggaggcc agcagggaca gcagtgtgtg tccagtttgt
     2341 cagccacacc ctgtgtacag gttaaagtta aagtttaagc gcggtagcct tcccccgacc
     2401 atgcctctgg agtttgtttg ctgcatcggc ggatgcgacg acaccctgag ggagatcctg
     2461 gacctgagat tttcaggggg cccccccagg gctagccagc cctctggccg cctgcaggct
     2521 aaccagtccc tgaacaggat ggacaacagc cagcaccccc agcctgctga cagcagacag
     2581 actgggtcct caaaggctct ggcccagacc ctcccaccac ccacggctgc tggtgaaagc
     2641 aattctgtga cctgcaactg tggccaggag gctgtgctgc tcactgtccg taaggagggc
     2701 cccaaccggg gccggcagtt ctttaagtgc aacggaggta gctgcaactt cttcctgtgg
     2761 gcagacagcc ccaatccggg agcaggaggg cctcctgcct tggcatatag acccctgggc
     2821 gcctccctgg gatgcccacc aggcccaggg atccacctag gtgggtttgg caaccctggt
     2881 gatggcagtg gtagtggcac atcctgcctt tgcagccagc cctccgtcac acggactgtg
     2941 cagaaggatg gacccaacaa ggggcgccag ttccacacat gtgccaagcc gagagagcag
     3001 cagtgtggct ttttccagtg ggtcgatgag aacaccgctc cagggacttc tggagccccg
     3061 tcctggacag gagacagagg aagaaccctg gagtcggaag ccagaagcaa aaggccccgg
     3121 gccagttcct cagacatggg gtccacagca aagaaacccc ggaaatgcag cctttgccac
     3181 cagcctggac acacccgtcc cttttgtcct cagaacagat gagctcaggg tagggtagag
     3241 aacgccactt tctcagacct gtcccctttg tgtttagaaa tgagttaacc aggaccaagt
     3301 ggccatttag tgtcctggaa acttagagga cagtgttggc ctttggagtc gggccttctt
     3361 gtgttaaggg gcacaaggtc cagatcactc tggagcaggc cagctctgct ggacagtgac
     3421 cctcttccca ggcctcagga gtgaccatag ccactgctga aaagtcacgc agctgctccc
     3481 tcggaccccc caaggatggt tgctgttagc agaggattgg tgcagtccca gctgaagccc
     3541 actgtgtgcc aaaggaagaa gctcccaggg ctgcttcctt cacctgcaga aagccccaag
     3601 tgagccacca gcactcatgg ggcagtccct gtccaggctg cccagggctt ctcatagacg
     3661 tcctgagaag gacggtgtaa tgcaaggaaa tggctgtggt aacactgatc cttcagaaga
     3721 agcttcattc cctcttaatc tagttaagcc aggacatcca gaattcattg ctttaataaa
     3781 gaacccaggc cgggtgcagt ggctcatgcc tgtaatccca gcactttggg aggctgtggg
     3841 gggcggattg cctgagctca ggagttcgag accagccagg gcaacatggt gaaaccctgt
     3901 ctctactaaa atacaaaaaa ttagccaggt gtggcggtgt gcgcctgtag acccggctac
     3961 tcaggaggct gaggcaggag aattgcttga acccgggagg cggaggttgc agttagctga
     4021 gattgcgcca ctggacgaca gagcgagact cagtctcaaa aaaaatttaa aaatttaaaa
     4081 aaataaaaag aatccaaccc cggcagggcg tggaggctca cacctgtaat ccagcactta
     4141 gggaggccaa ggctggagga ttgcttgaga tcaggagttc aagaccagcc tgggcaacat
     4201 agttagacct tgtctctatt tttaaaaata taacaagcac agttgtacac gtctttagtt
     4261 cagctgctca ggaggctgaa gtgggaggat cctttgaacc caagagtttg aggctgcagc
     4321 aagccatgat cacaccactg cactccagcc tgggtgacag agtaagaccc tgtctcaaac
     4381 tttttttaaa atgaaagaat ccaacctttt tttactctga cctgcgagag tgcagagggt
     4441 ctggggaaca tttgcagaag caacaggtac cagccagtgc tggaaggagc tcaccctggg
     4501 aggtctcgtc agcctctgtc cttcatggct gtcccttgtg tcccatgtgg agagcccttc
     4561 ctccctttcc acatggtaag cactgagccc aatttcttct caccccacag atggtccctc
     4621 agagcagaga tgtctaatga aaggttcaga ttcagatcac taactttcca tcttccactt
     4681 tttccagtgg tggccatgtt cccccgtttg ccttcacaaa aaccttgtga ataatacaag
     4741 ccatatggac tctgatttac agtttagaag atgagcagag gtgggtgtga gttgcccagt
     4801 catgttgcta gttgttgaag aaactaggat tgttctcagg tcttgggctc ctggcccata
     4861 gaccagtggc tctgtgttct gatggggtat tggggaggat ttttacaaat gcaggttcct
     4921 gagattgttc ctggaacatc tccgagtggg tgtgggttgt ggccctgcgt gtgtgatttt
     4981 gcctatccca gctccggggt accaccaacc actttttgtc tctgtgggtt tacctactct
     5041 ggatattttg tttaaatgga atcataccag gctgggcaca gtggctcacg cctgtaatcc
     5101 tagcactttg ggaggccaag gtgggcagat cacctgaggt caggagttcg agaccagcct
     5161 gaccaatatg atgaaacccc gtctctaaaa aaatacaaaa attagccggg cgtggtgtca
     5221 ggcacctgta atcccagcta ctcaggaagc agaggttgca gtgagctgag atcgggccat
     5281 tgcactccag cctgggcaaa aagagtgaaa ctctgtctca agaaaaaaaa aaaaatgaaa
     5341 tcctaccata catgacatcc tgtgtctggt ttctttgact tggctgatgt ttgtcaggct
     5401 catccacatt gtagcatgtc agtcctcgat gtttatgata agctgatagc ccactgtatg
     5461 aatataccac acgattatcc atttgtcagt tgatggacat tgggttgttt ccactttttg
     5521 gctattgtga atagcactgt tgtgaacatt ggtgtatgag tgtctgatga cttgttttca
     5581 gttattttgg gcatatgccg aggaatggaa ttgtagtttt ataccataac tccatgtttt
     5641 gactttttga ggaacctaca aattttccac agtggatgca ccactttaca cccaccagca
     5701 gcacaccagg gttactgctt ctccatatcc ttgtgaacac ttgttccttt ccttttcttg
     5761 tattatagcc atcctagtgt tttgtcatcc tagtgggtat gaagtggcgt gtcattgtgg
     5821 ttttgttttg cattttccta gtcattaatg acattgacca tctttccaca ggcttactgg
     5881 ccattggtat acattctttg gagaatgtcg atttaagttc ttcacccatt ttttattttt
     5941 tattttttat ttttttttga gatggagttt cgctcttgtg cccaggctgg agtgcagtgg
     6001 tgcgatcttg gctcactgca acctctgcct cctgggttca agcagttctc ctgcctcagc
     6061 ctcccaagta gctggaatta caggcatctg cctccacacc cagctaattt gtttagtatt
     6121 tttagtaaag acggggtttc accatgttgg ccaggctggt ctcaaactcg tgacctcagg
     6181 tgatctacca gcctcggcct cccaaagtgc taggattaac aggcaggagc catcacacct
     6241 ggtctcttcc cccattttta aattgtgttg tctgtctttg ttgttgagtt gtaggagttc
     6301 ttaatagttt ctggatatca gatgcctttg tggttttcac atctctgcag gcaactgtgt
     6361 gtctcaaaag caaactgcag gattgggctg caccatggac tgggccaggc atctgagatc
     6421 agacagaccc aggctcagct cccacttcct ggcctcttct gccaccctgg gtgtgcagcc
     6481 tcatccttgg agccccagtt gcctcatccg taatggggtg tccttgttca tattgaggct
     6541 ctctagtgaa ggcttacggt caggcacgac tgcaaattaa agcttcacaa atcaaa
//
