LOCUS       NM_001320759            6522 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript
            variant 2, mRNA.
ACCESSION   NM_001320759 XM_005256776
VERSION     NM_001320759.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Erdinc,D., Rodriguez-Luis,A., Fassad,M.R., Mackenzie,S.,
            Watson,C.M., Valenzuela,S., Xie,X., Menger,K.E., Sergeant,K.,
            Craig,K., Hopton,S., Falkous,G., Poulton,J., Garcia-Moreno,H.,
            Giunti,P., de Moura Aschoff,C.A., Morales Saute,J.A., Kirby,A.J.,
            Toro,C., Wolfe,L., Novacic,D., Greenbaum,L., Eliyahu,A., Barel,O.,
            Anikster,Y., McFarland,R., Gorman,G.S., Schaefer,A.M.,
            Gustafsson,C.M., Taylor,R.W., Falkenberg,M. and Nicholls,T.J.
  CONSRTM   Genomics England Research Consortium
  TITLE     Pathological variants in TOP3A cause distinct disorders of
            mitochondrial and nuclear genome stability
  JOURNAL   EMBO Mol Med 15 (5), e16775 (2023)
   PUBMED   37013609
  REMARK    GeneRIF: Pathological variants in TOP3A cause distinct disorders of
            mitochondrial and nuclear genome stability.
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Soniat,M.M., Nguyen,G., Kuo,H.C. and Finkelstein,I.J.
  TITLE     The MRN complex and topoisomerase IIIa-RMI1/2 synchronize DNA
            resection motor proteins
  JOURNAL   J Biol Chem 299 (2), 102802 (2023)
   PUBMED   36529288
  REMARK    GeneRIF: The MRN complex and topoisomerase IIIa-RMI1/2 synchronize
            DNA resection motor proteins.
REFERENCE   3  (bases 1 to 6522)
  AUTHORS   Menger,K.E., Chapman,J., Diaz-Maldonado,H., Khazeem,M.M., Deen,D.,
            Erdinc,D., Casement,J.W., Di Leo,V., Pyle,A., Rodriguez-Luis,A.,
            Cowell,I.G., Falkenberg,M., Austin,C.A. and Nicholls,T.J.
  TITLE     Two type I topoisomerases maintain DNA topology in human
            mitochondria
  JOURNAL   Nucleic Acids Res 50 (19), 11154-11174 (2022)
   PUBMED   36215039
  REMARK    GeneRIF: Two type I topoisomerases maintain DNA topology in human
            mitochondria.
REFERENCE   4  (bases 1 to 6522)
  AUTHORS   de Nonneville,A., Salas,S., Bertucci,F., Sobinoff,A.P.,
            Adelaide,J., Guille,A., Finetti,P., Noble,J.R., Churikov,D.,
            Chaffanet,M., Lavit,E., Pickett,H.A., Bouvier,C., Birnbaum,D.,
            Reddel,R.R. and Geli,V.
  TITLE     TOP3A amplification and ATRX inactivation are mutually exclusive
            events in pediatric osteosarcomas using ALT
  JOURNAL   EMBO Mol Med 14 (10), e15859 (2022)
   PUBMED   35920001
  REMARK    GeneRIF: TOP3A amplification and ATRX inactivation are mutually
            exclusive events in pediatric osteosarcomas using ALT.
REFERENCE   5  (bases 1 to 6522)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 6522)
  AUTHORS   Shimamoto,A., Nishikawa,K., Kitao,S. and Furuichi,Y.
  TITLE     Human RecQ5beta, a large isomer of RecQ5 DNA helicase, localizes in
            the nucleoplasm and interacts with topoisomerases 3alpha and 3beta
  JOURNAL   Nucleic Acids Res 28 (7), 1647-1655 (2000)
   PUBMED   10710432
REFERENCE   7  (bases 1 to 6522)
  AUTHORS   Goulaouic,H., Roulon,T., Flamand,O., Grondard,L., Lavelle,F. and
            Riou,J.F.
  TITLE     Purification and characterization of human DNA topoisomerase
            IIIalpha
  JOURNAL   Nucleic Acids Res 27 (12), 2443-2450 (1999)
   PUBMED   10352172
REFERENCE   8  (bases 1 to 6522)
  AUTHORS   Kim,J.C., Yoon,J.B., Koo,H.S. and Chung,I.K.
  TITLE     Cloning and characterization of the 5'-flanking region for the
            human topoisomerase III gene
  JOURNAL   J Biol Chem 273 (40), 26130-26137 (1998)
   PUBMED   9748294
REFERENCE   9  (bases 1 to 6522)
  AUTHORS   Fritz,E., Elsea,S.H., Patel,P.I. and Meyn,M.S.
  TITLE     Overexpression of a truncated human topoisomerase III partially
            corrects multiple aspects of the ataxia-telangiectasia phenotype
  JOURNAL   Proc Natl Acad Sci U S A 94 (9), 4538-4542 (1997)
   PUBMED   9114025
REFERENCE   10 (bases 1 to 6522)
  AUTHORS   Hanai,R., Caron,P.R. and Wang,J.C.
  TITLE     Human TOP3: a single-copy gene encoding DNA topoisomerase III
  JOURNAL   Proc Natl Acad Sci U S A 93 (8), 3653-3657 (1996)
   PUBMED   8622991
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BM462184.1, AK296437.1,
            BY996313.1, AF086042.1, AC127537.8 and AW294300.1.
            
            On May 31, 2019 this sequence version replaced NM_001320759.1.
            
            Summary: This gene encodes a DNA topoisomerase, an enzyme that
            controls and alters the topologic states of DNA during
            transcription. This enzyme catalyzes the transient breaking and
            rejoining of a single strand of DNA which allows the strands to
            pass through one another, thus reducing the number of supercoils
            and altering the topology of DNA. This enzyme forms a complex with
            BLM which functions in the regulation of recombination in somatic
            cells. Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Mar 2016].
            
            Transcript Variant: This variant (2) lacks an alternate exon in the
            5' coding region, resulting in a novel 5' UTR and the use of an
            alternate start codon, compared to variant 1. The encoded isoform
            (2) has a distinct N-terminus and is shorter, compared to isoform
            1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.222873.1,
                                           SRR1803612.112571.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-70                BM462184.1         100-169
            71-3226             AK296437.1         1-3156
            3227-3780           BY996313.1         76-629
            3781-4029           AF086042.1         296-544
            4030-6289           AC127537.8         45900-48159         c
            6290-6522           AW294300.1         15-247              c
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p11.2"
     gene            1..6522
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /note="DNA topoisomerase III alpha"
                     /db_xref="GeneID:7156"
                     /db_xref="HGNC:HGNC:11992"
                     /db_xref="MIM:601243"
     exon            1..396
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            397..456
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     CDS             428..3148
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /EC_number="5.6.2.1"
                     /note="isoform 2 is encoded by transcript variant 2; topo
                     III-alpha; DNA topoisomerase 3-alpha; zinc finger,
                     GRF-type containing 7; topoisomerase (DNA) III alpha"
                     /codon_start=1
                     /product="DNA topoisomerase 3-alpha isoform 2"
                     /protein_id="NP_001307688.1"
                     /db_xref="GeneID:7156"
                     /db_xref="HGNC:HGNC:11992"
                     /db_xref="MIM:601243"
                     /translation="MNLIIICMARQSCNPLVLFEAEIEKYCPENFVDIKKTLERETRQ
                     CQALVIWTDCDREGENIGFEIIHVCKAVKPNLQVLRARFSEITPHAVRTACENLTEPD
                     QRVSDAVDVRQELDLRIGAAFTRFQTLRLQRIFPEVLAEQLISYGSCQFPTLGFVVER
                     FKAIQAFVPEIFHRIKVTHDHKDGIVEFNWKRHRLFNHTACLVLYQLCVEDPMATVVE
                     VRSKPKSKWRPQALDTVELEKLASRKLRINAKETMRIAEKLYTQGYISYPRTETNIFP
                     RDLNLTVLVEQQTPDPRWGAFAQSILERGGPTPRNGNKSDQAHPPIHPTKYTNNLQGD
                     EQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLMILARNYLDVYPYDHWS
                     DKILPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETI
                     KARMYVGLTPDKRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKF
                     VVLRQQVQKYKQVFIEAVAKAKKLDEALAQYFGNGTELAQQEDIYPAMPEPIRKCPQC
                     NKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSVLEASRDSSVCPVCQPHPVYRLKLK
                     FKRGSLPPTMPLEFVCCIGGCDDTLREILDLRFSGGPPRASQPSGRLQANQSLNRMDN
                     SQHPQPADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQF
                     FKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGS
                     GTSCLCSQPSVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWT
                     GDRGRTLESEARSKRPRASSSDMGSTAKKPRKCSLCHQPGHTRPFCPQNR"
     exon            457..532
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            533..641
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            642..785
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            786..956
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1057
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            1058..1132
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..1215
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1423
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            1424..1609
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            1610..1739
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            1740..1853
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            1854..2019
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            2020..2163
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            2164..2286
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            2287..2969
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     exon            2970..6522
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3701..3706
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /note="hexamer: AATAAA"
     polyA_site      3720
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /note="major polyA site"
     polyA_site      6188
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
     regulatory      6502..6507
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /note="hexamer: ATTAAA"
     polyA_site      6522
                     /gene="TOP3A"
                     /gene_synonym="MGRISCE2; PEOB5; TOP3; ZGRF7"
                     /note="major polyA site"
ORIGIN      
        1 ggaagttcca cgccaagata ctggattcgg tgctgaggcg gcggctgcgg cacgggaaag
       61 gctcagtgac tgaagctcca aaggccagca ggctggtggg gacgtgaccg aagcgaggct
      121 ctggttccct ttcggtgggc gccatttgag cctcatctct ggcttcccca ggatgcgccg
      181 gcagccgggg agcggctccg ggcgcgaggt ctgaggatga tctttcctgt cgcccgctac
      241 gcgctccggt ggctgcgacg gcccgaagac cgtgcctttt cccgcgccgc catggagatg
      301 gccctccgag gcgtgcggaa agtcctctgt gtggccgaaa aaaacgacgc ggccaagggg
      361 atcgccgacc tgctgtcaaa cggtcgcatg aggcggagag aaggactttc aaaattcaac
      421 aagatctatg aatttgatta tcatctgtat ggccaggcag agctgcaacc ctcttgtcct
      481 ctttgaagca gaaattgaaa agtactgccc agagaatttt gtagacatca agaaaacttt
      541 ggaacgagag actcgccagt gccaggctct ggtgatctgg actgactgtg atagagaagg
      601 cgaaaacatc gggtttgaga ttatccacgt gtgtaaggct gtaaagccca atctgcaggt
      661 gttgcgagcc cgattctctg agatcacacc ccatgccgtc aggacagctt gtgaaaacct
      721 gaccgagcct gatcagaggg tgagcgatgc tgtggatgtg aggcaggagc tggacctgag
      781 gattggagct gcctttacta ggttccagac cctgcggctt cagaggattt ttcctgaggt
      841 gctggcagag cagctcatca gttacggcag ctgccagttc cccacactgg gctttgtggt
      901 ggagcggttc aaagccattc aggcttttgt accagaaatc ttccacagaa ttaaagtaac
      961 tcatgaccac aaagatggta tcgtagaatt caactggaaa aggcatcgac tctttaacca
     1021 cacggcttgc ctagttctct atcagttgtg tgtggaggat cccatggcaa ctgtggtaga
     1081 ggtcagatct aagcccaaga gcaagtggcg gcctcaagcc ttggacactg tggagcttga
     1141 gaagctggct tctcgaaagt tgagaataaa tgctaaagaa accatgagga ttgctgagaa
     1201 gctctacact caagggtaca tcagctatcc ccgaacagaa acaaacattt ttcccagaga
     1261 cttaaacctg acggtgttgg tggaacagca gacccccgat ccacgctggg gggcctttgc
     1321 ccagagcatt ctagagcggg gtggtcccac cccacgcaat gggaacaagt ctgaccaagc
     1381 tcaccctccc attcacccca ccaaatacac caacaactta cagggagatg aacagcgact
     1441 gtacgagttt attgttcgcc atttcctggc ttgctgctcc caggatgctc aggggcagga
     1501 gaccacagtg gagatcgaca tcgctcagga acgctttgtg gcccatggcc tcatgattct
     1561 ggcccgaaac tatctggatg tgtatccata tgatcactgg agtgacaaga tcctccctgt
     1621 ctatgagcaa ggatcccact ttcagcccag caccgtggag atggtggacg gggagaccag
     1681 cccacccaag ctgctcaccg aggccgacct cattgccctc atggagaagc atggcattgg
     1741 tacggatgcc actcatgcgg agcacatcga gaccatcaaa gcccggatgt acgtgggcct
     1801 caccccagac aagcggttcc tccctgggca cctgggcatg ggacttgtgg aaggttatga
     1861 ttccatgggc tatgaaatgt ctaagcctga cctccgggct gaactggaag ctgatctgaa
     1921 gctgatctgt gatggcaaaa aggacaaatt tgtggttcta aggcagcaag tgcagaaata
     1981 caagcaggtt ttcattgaag cggtggctaa agcaaagaaa ttggacgagg ccttggccca
     2041 gtactttggg aatgggacag agttggccca gcaagaagat atctacccag ccatgccaga
     2101 gcccatcagg aagtgcccac agtgcaacaa ggacatggtc cttaagacca agaagaatgg
     2161 cgggttctac ctcagctgca tgggtttccc agagtgtcgc tcagctgtgt ggcttcctga
     2221 ctcggtgctg gaggccagca gggacagcag tgtgtgtcca gtttgtcagc cacaccctgt
     2281 gtacaggtta aagttaaagt ttaagcgcgg tagccttccc ccgaccatgc ctctggagtt
     2341 tgtttgctgc atcggcggat gcgacgacac cctgagggag atcctggacc tgagattttc
     2401 agggggcccc cccagggcta gccagccctc tggccgcctg caggctaacc agtccctgaa
     2461 caggatggac aacagccagc acccccagcc tgctgacagc agacagactg ggtcctcaaa
     2521 ggctctggcc cagaccctcc caccacccac ggctgctggt gaaagcaatt ctgtgacctg
     2581 caactgtggc caggaggctg tgctgctcac tgtccgtaag gagggcccca accggggccg
     2641 gcagttcttt aagtgcaacg gaggtagctg caacttcttc ctgtgggcag acagccccaa
     2701 tccgggagca ggagggcctc ctgccttggc atatagaccc ctgggcgcct ccctgggatg
     2761 cccaccaggc ccagggatcc acctaggtgg gtttggcaac cctggtgatg gcagtggtag
     2821 tggcacatcc tgcctttgca gccagccctc cgtcacacgg actgtgcaga aggatggacc
     2881 caacaagggg cgccagttcc acacatgtgc caagccgaga gagcagcagt gtggcttttt
     2941 ccagtgggtc gatgagaaca ccgctccagg gacttctgga gccccgtcct ggacaggaga
     3001 cagaggaaga accctggagt cggaagccag aagcaaaagg ccccgggcca gttcctcaga
     3061 catggggtcc acagcaaaga aaccccggaa atgcagcctt tgccaccagc ctggacacac
     3121 ccgtcccttt tgtcctcaga acagatgagc tcagggtagg gtagagaacg ccactttctc
     3181 agacctgtcc cctttgtgtt tagaaatgag ttaaccagga ccaagtggcc atttagtgtc
     3241 ctggaaactt agaggacagt gttggccttt ggagtcgggc cttcttgtgt taaggggcac
     3301 aaggtccaga tcactctgga gcaggccagc tctgctggac agtgaccctc ttcccaggcc
     3361 tcaggagtga ccatagccac tgctgaaaag tcacgcagct gctccctcgg accccccaag
     3421 gatggttgct gttagcagag gattggtgca gtcccagctg aagcccactg tgtgccaaag
     3481 gaagaagctc ccagggctgc ttccttcacc tgcagaaagc cccaagtgag ccaccagcac
     3541 tcatggggca gtccctgtcc aggctgccca gggcttctca tagacgtcct gagaaggacg
     3601 gtgtaatgca aggaaatggc tgtggtaaca ctgatccttc agaagaagct tcattccctc
     3661 ttaatctagt taagccagga catccagaat tcattgcttt aataaagaac ccaggccggg
     3721 tgcagtggct catgcctgta atcccagcac tttgggaggc tgtggggggc ggattgcctg
     3781 agctcaggag ttcgagacca gccagggcaa catggtgaaa ccctgtctct actaaaatac
     3841 aaaaaattag ccaggtgtgg cggtgtgcgc ctgtagaccc ggctactcag gaggctgagg
     3901 caggagaatt gcttgaaccc gggaggcgga ggttgcagtt agctgagatt gcgccactgg
     3961 acgacagagc gagactcagt ctcaaaaaaa atttaaaaat ttaaaaaaat aaaaagaatc
     4021 caaccccggc agggcgtgga ggctcacacc tgtaatccag cacttaggga ggccaaggct
     4081 ggaggattgc ttgagatcag gagttcaaga ccagcctggg caacatagtt agaccttgtc
     4141 tctattttta aaaatataac aagcacagtt gtacacgtct ttagttcagc tgctcaggag
     4201 gctgaagtgg gaggatcctt tgaacccaag agtttgaggc tgcagcaagc catgatcaca
     4261 ccactgcact ccagcctggg tgacagagta agaccctgtc tcaaactttt tttaaaatga
     4321 aagaatccaa ccttttttta ctctgacctg cgagagtgca gagggtctgg ggaacatttg
     4381 cagaagcaac aggtaccagc cagtgctgga aggagctcac cctgggaggt ctcgtcagcc
     4441 tctgtccttc atggctgtcc cttgtgtccc atgtggagag cccttcctcc ctttccacat
     4501 ggtaagcact gagcccaatt tcttctcacc ccacagatgg tccctcagag cagagatgtc
     4561 taatgaaagg ttcagattca gatcactaac tttccatctt ccactttttc cagtggtggc
     4621 catgttcccc cgtttgcctt cacaaaaacc ttgtgaataa tacaagccat atggactctg
     4681 atttacagtt tagaagatga gcagaggtgg gtgtgagttg cccagtcatg ttgctagttg
     4741 ttgaagaaac taggattgtt ctcaggtctt gggctcctgg cccatagacc agtggctctg
     4801 tgttctgatg gggtattggg gaggattttt acaaatgcag gttcctgaga ttgttcctgg
     4861 aacatctccg agtgggtgtg ggttgtggcc ctgcgtgtgt gattttgcct atcccagctc
     4921 cggggtacca ccaaccactt tttgtctctg tgggtttacc tactctggat attttgttta
     4981 aatggaatca taccaggctg ggcacagtgg ctcacgcctg taatcctagc actttgggag
     5041 gccaaggtgg gcagatcacc tgaggtcagg agttcgagac cagcctgacc aatatgatga
     5101 aaccccgtct ctaaaaaaat acaaaaatta gccgggcgtg gtgtcaggca cctgtaatcc
     5161 cagctactca ggaagcagag gttgcagtga gctgagatcg ggccattgca ctccagcctg
     5221 ggcaaaaaga gtgaaactct gtctcaagaa aaaaaaaaaa atgaaatcct accatacatg
     5281 acatcctgtg tctggtttct ttgacttggc tgatgtttgt caggctcatc cacattgtag
     5341 catgtcagtc ctcgatgttt atgataagct gatagcccac tgtatgaata taccacacga
     5401 ttatccattt gtcagttgat ggacattggg ttgtttccac tttttggcta ttgtgaatag
     5461 cactgttgtg aacattggtg tatgagtgtc tgatgacttg ttttcagtta ttttgggcat
     5521 atgccgagga atggaattgt agttttatac cataactcca tgttttgact ttttgaggaa
     5581 cctacaaatt ttccacagtg gatgcaccac tttacaccca ccagcagcac accagggtta
     5641 ctgcttctcc atatccttgt gaacacttgt tcctttcctt ttcttgtatt atagccatcc
     5701 tagtgttttg tcatcctagt gggtatgaag tggcgtgtca ttgtggtttt gttttgcatt
     5761 ttcctagtca ttaatgacat tgaccatctt tccacaggct tactggccat tggtatacat
     5821 tctttggaga atgtcgattt aagttcttca cccatttttt attttttatt ttttattttt
     5881 ttttgagatg gagtttcgct cttgtgccca ggctggagtg cagtggtgcg atcttggctc
     5941 actgcaacct ctgcctcctg ggttcaagca gttctcctgc ctcagcctcc caagtagctg
     6001 gaattacagg catctgcctc cacacccagc taatttgttt agtattttta gtaaagacgg
     6061 ggtttcacca tgttggccag gctggtctca aactcgtgac ctcaggtgat ctaccagcct
     6121 cggcctccca aagtgctagg attaacaggc aggagccatc acacctggtc tcttccccca
     6181 tttttaaatt gtgttgtctg tctttgttgt tgagttgtag gagttcttaa tagtttctgg
     6241 atatcagatg cctttgtggt tttcacatct ctgcaggcaa ctgtgtgtct caaaagcaaa
     6301 ctgcaggatt gggctgcacc atggactggg ccaggcatct gagatcagac agacccaggc
     6361 tcagctccca cttcctggcc tcttctgcca ccctgggtgt gcagcctcat ccttggagcc
     6421 ccagttgcct catccgtaat ggggtgtcct tgttcatatt gaggctctct agtgaaggct
     6481 tacggtcagg cacgactgca aattaaagct tcacaaatca aa
//
