LOCUS       NM_003286               3734 bp    mRNA    linear   PRI 19-AUG-2024
DEFINITION  Homo sapiens DNA topoisomerase I (TOP1), mRNA.
ACCESSION   NM_003286
VERSION     NM_003286.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Hu,H., Shen,S., Wu,J. and Ma,L.
  TITLE     CircTOP1 targeted regulation of PTBP1 expression promotes the
            progression of coronary artery calcification
  JOURNAL   Exp Cell Res 440 (2), 114147 (2024)
   PUBMED   38944174
  REMARK    GeneRIF: CircTOP1 targeted regulation of PTBP1 expression promotes
            the progression of coronary artery calcification.
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Duardo,R.C., Marinello,J., Russo,M., Morelli,S., Pepe,S.,
            Guerra,F., Gomez-Gonzalez,B., Aguilera,A. and Capranico,G.
  TITLE     Human DNA topoisomerase I poisoning causes R loop-mediated genome
            instability attenuated by transcription factor IIS
  JOURNAL   Sci Adv 10 (21), eadm8196 (2024)
   PUBMED   38787953
  REMARK    GeneRIF: Human DNA topoisomerase I poisoning causes R loop-mediated
            genome instability attenuated by transcription factor IIS.
REFERENCE   3  (bases 1 to 3734)
  AUTHORS   Mulalin,K., Mahakkanukrauh,A., Suwannaroj,S., Pongkulkiat,P.,
            Onchan,T., Kasa,S. and Foocharoen,C.
  TITLE     Levels of anti-topoisomerase I antibody correlated with short onset
            of cardiopulmonary involvement in Thai systemic sclerosis patients
  JOURNAL   Sci Rep 14 (1), 10354 (2024)
   PUBMED   38710779
  REMARK    GeneRIF: Levels of anti-topoisomerase I antibody correlated with
            short onset of cardiopulmonary involvement in Thai systemic
            sclerosis patients.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3734)
  AUTHORS   Liang,H.T., Yan,J.Y., Yao,H.J., Zhang,X.N., Xing,Z.M., Liu,L.,
            Chen,Y.Q., Li,G.R., Huang,J., He,Y.D. and Zheng,K.W.
  TITLE     G-quadruplexes on chromosomal DNA negatively regulates
            topoisomerase 1 activity
  JOURNAL   Nucleic Acids Res 52 (5), 2142-2156 (2024)
   PUBMED   38340342
  REMARK    GeneRIF: G-quadruplexes on chromosomal DNA negatively regulates
            topoisomerase 1 activity.
REFERENCE   5  (bases 1 to 3734)
  AUTHORS   Cameron,D.P., Grosser,J., Ladigan,S., Kuzin,V., Iliopoulou,E.,
            Wiegard,A., Benredjem,H., Jackson,K., Liffers,S.T., Lueong,S.,
            Cheung,P.F., Vangala,D., Pohl,M., Viebahn,R., Teschendorf,C.,
            Wolters,H., Usta,S., Geng,K., Kutter,C., Arsenian-Henriksson,M.,
            Siveke,J.T., Tannapfel,A., Schmiegel,W., Hahn,S.A. and Baranello,L.
  TITLE     Coinhibition of topoisomerase 1 and BRD4-mediated pause release
            selectively kills pancreatic cancer via readthrough transcription
  JOURNAL   Sci Adv 9 (41), eadg5109 (2023)
   PUBMED   37831776
  REMARK    GeneRIF: Coinhibition of topoisomerase 1 and BRD4-mediated pause
            release selectively kills pancreatic cancer via readthrough
            transcription.
REFERENCE   6  (bases 1 to 3734)
  AUTHORS   Kubota,N., Kanzawa,F., Nishio,K., Takeda,Y., Ohmori,T.,
            Fujiwara,Y., Terashima,Y. and Saijo,N.
  TITLE     Detection of topoisomerase I gene point mutation in CPT-11
            resistant lung cancer cell line
  JOURNAL   Biochem Biophys Res Commun 188 (2), 571-577 (1992)
   PUBMED   1332703
REFERENCE   7  (bases 1 to 3734)
  AUTHORS   Zhou,B.S., Beidler,D.R. and Cheng,Y.C.
  TITLE     Identification of antisense RNA transcripts from a human DNA
            topoisomerase I pseudogene
  JOURNAL   Cancer Res 52 (15), 4280-4285 (1992)
   PUBMED   1339303
REFERENCE   8  (bases 1 to 3734)
  AUTHORS   Kunze,N., Yang,G.C., Dolberg,M., Sundarp,R., Knippers,R. and
            Richter,A.
  TITLE     Structure of the human type I DNA topoisomerase gene
  JOURNAL   J Biol Chem 266 (15), 9610-9616 (1991)
   PUBMED   1851751
REFERENCE   9  (bases 1 to 3734)
  AUTHORS   Tamura,H., Kohchi,C., Yamada,R., Ikeda,T., Koiwai,O., Patterson,E.,
            Keene,J.D., Okada,K., Kjeldsen,E., Nishikawa,K. et al.
  TITLE     Molecular cloning of a cDNA of a camptothecin-resistant human DNA
            topoisomerase I and identification of mutation sites
  JOURNAL   Nucleic Acids Res 19 (1), 69-75 (1991)
   PUBMED   1849260
REFERENCE   10 (bases 1 to 3734)
  AUTHORS   Priel,E., Showalter,S.D., Roberts,M., Oroszlan,S., Segal,S.,
            Aboud,M. and Blair,D.G.
  TITLE     Topoisomerase I activity associated with human immunodeficiency
            virus (HIV) particles and equine infectious anemia virus core
  JOURNAL   EMBO J 9 (12), 4167-4172 (1990)
   PUBMED   2174357
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA690829.1, AK292943.1 and
            BC000943.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_003286.3.
            
            Summary: This gene encodes a DNA topoisomerase, an enzyme that
            controls and alters the topologic states of DNA during
            transcription. This enzyme catalyzes the transient breaking and
            rejoining of a single strand of DNA which allows the strands to
            pass through one another, thus altering the topology of DNA. This
            gene is localized to chromosome 20 and has pseudogenes which reside
            on chromosomes 1 and 22. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U07806.1, SRR1660803.104236.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361337.3/ ENSP00000354522.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 DA690829.1         5-5
            2-2995              AK292943.1         1-2994
            2996-3734           BC000943.2         983-1721
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q12"
     gene            1..3734
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /note="DNA topoisomerase I"
                     /db_xref="GeneID:7150"
                     /db_xref="HGNC:HGNC:11986"
                     /db_xref="MIM:126420"
     exon            1..279
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     CDS             247..2544
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /EC_number="5.6.2.1"
                     /note="type I DNA topoisomerase; topoisomerase (DNA) I;
                     Scl-70 antigen"
                     /codon_start=1
                     /product="DNA topoisomerase 1"
                     /protein_id="NP_003277.1"
                     /db_xref="CCDS:CCDS13312.1"
                     /db_xref="GeneID:7150"
                     /db_xref="HGNC:HGNC:11986"
                     /db_xref="MIM:126420"
                     /translation="MSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKEKDR
                     EKSKHSNSEHKDSEKKHKEKEKTKHKDGSSEKHKDKHKDRDKEKRKEEKVRASGDAKI
                     KKEKENGFSSPPQIKDEPEDDGYFVPPKEDIKPLKRPRDEDDADYKPKKIKTEDTKKE
                     KKRKLEEEEDGKLKKPKNKDKDKKVPEPDNKKKKPKKEEEQKWKWWEEERYPEGIKWK
                     FLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRK
                     NFFKDWRKEMTNEEKNIITNLSKCDFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKL
                     LKEYGFCIMDNHKERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKV
                     PSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRL
                     KKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLR
                     VEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFD
                     RLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENIPAKILSYNRANR
                     AVAILCNHQRAPPKTFEKSMMNLQTKIDAKKEQLADARRDLKSAKADAKVMKDAKTKK
                     VVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPI
                     EKIYNKTQREKFAWAIDMADEDYEF"
     exon            280..304
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     exon            305..401
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     exon            402..525
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     exon            526..581
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     exon            582..677
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     exon            678..753
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     exon            754..860
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     exon            861..976
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1098
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1221
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1409
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1554
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     exon            1555..1698
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     exon            1699..1884
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     exon            1885..1953
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     exon            1954..2068
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     exon            2069..2196
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     exon            2197..2291
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     exon            2292..2441
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     exon            2442..3734
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3709..3714
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /note="hexamer: AATAAA"
     polyA_site      3734
                     /gene="TOP1"
                     /gene_synonym="TOPI"
                     /note="major polyA site"
ORIGIN      
        1 caaatgcgaa cttaggctgt tacacaactg ctggggtctg ttctcgccgc ccgcccggca
       61 gtcaggcagc gtcgccgccg tggtagcagc ctcagccgtt tctggagtct cgggcccaca
      121 gtcaccgccg cttacctgcg cctcctcgag cctccggagt ccccgtccgc ccgcacaggc
      181 cggttcgccg tctgcgtctc ccccacgccg cctcgcctgc cgccgcgctc gtccctccgg
      241 gccgacatga gtggggacca cctccacaac gattcccaga tcgaagcgga tttccgattg
      301 aatgattctc ataaacacaa agataaacac aaagatcgag aacaccggca caaagaacac
      361 aagaaggaga aggaccggga aaagtccaag catagcaaca gtgaacataa agattctgaa
      421 aagaaacaca aagagaagga gaagaccaaa cacaaagatg gaagctcaga aaagcataaa
      481 gacaaacata aagacagaga caaggaaaaa cgaaaagagg aaaaggttcg agcctctggg
      541 gatgcaaaaa taaagaagga gaaggaaaat ggcttctcta gtccaccaca aattaaagat
      601 gaacctgaag atgatggcta ttttgttcct cctaaagagg atataaagcc attaaagaga
      661 cctcgagatg aggatgatgc tgattataaa cctaagaaaa ttaaaacaga agataccaag
      721 aaggagaaga aaagaaaact agaagaagaa gaggatggta aattgaaaaa acccaagaat
      781 aaagataaag ataaaaaagt tcctgagcca gataacaaga aaaagaagcc gaagaaagaa
      841 gaggaacaga agtggaaatg gtgggaagaa gagcgctatc ctgaaggcat caagtggaaa
      901 ttcctagaac ataaaggtcc agtatttgcc ccaccatatg agcctcttcc agagaatgtc
      961 aagttttatt atgatggtaa agtcatgaag ctgagcccca aagcagagga agtagctacg
     1021 ttctttgcaa aaatgctcga ccatgaatat actaccaagg aaatatttag gaaaaatttc
     1081 tttaaagact ggagaaagga aatgactaat gaagagaaga atattatcac caacctaagc
     1141 aaatgtgatt ttacccagat gagccagtat ttcaaagccc agacggaagc tcggaaacag
     1201 atgagcaagg aagagaaact gaaaatcaaa gaggagaatg aaaaattact gaaagaatat
     1261 ggattctgta ttatggataa ccacaaagag aggattgcta acttcaagat agagcctcct
     1321 ggacttttcc gtggccgcgg caaccacccc aagatgggca tgctgaagag acgaatcatg
     1381 cccgaggata taatcatcaa ctgtagcaaa gatgccaagg ttccttctcc tcctccagga
     1441 cataagtgga aagaagtccg gcatgataac aaggttactt ggctggtttc ctggacagag
     1501 aacatccaag gttccattaa atacatcatg cttaacccta gttcacgaat caagggtgag
     1561 aaggactggc agaaatacga gactgctcgg cggctgaaaa aatgtgtgga caagatccgg
     1621 aaccagtatc gagaagactg gaagtccaaa gagatgaaag tccggcagag agctgtagcc
     1681 ctgtacttca tcgacaagct tgctctgaga gcaggcaatg aaaaggagga aggagaaaca
     1741 gcggacactg tgggctgctg ctcacttcgt gtggagcaca tcaatctaca cccagagttg
     1801 gatggtcagg aatatgtggt agagtttgac ttcctcggga aggactccat cagatactat
     1861 aacaaggtcc ctgttgagaa acgagttttt aagaacctac aactatttat ggagaacaag
     1921 cagcccgagg atgatctttt tgatagactc aatactggta ttctgaataa gcatcttcag
     1981 gatctcatgg agggcttgac agccaaggta ttccgtacat acaatgcctc catcacgcta
     2041 cagcagcagc taaaagaact gacagccccg gatgagaaca tcccagcgaa gatcctttct
     2101 tataaccgtg ccaatcgagc tgttgcaatt ctttgtaacc atcagagggc accaccaaaa
     2161 acttttgaga agtctatgat gaacttgcaa actaagattg atgccaagaa ggaacagcta
     2221 gcagatgccc ggagagacct gaaaagtgct aaggctgatg ccaaggtcat gaaggatgca
     2281 aagacgaaga aggtagtaga gtcaaagaag aaggctgttc agagactgga ggaacagttg
     2341 atgaagctgg aagttcaagc cacagaccga gaggaaaata aacagattgc cctgggaacc
     2401 tccaaactca attatctgga ccctaggatc acagtggctt ggtgcaagaa gtggggtgtc
     2461 ccaattgaga agatttacaa caaaacccag cgggagaagt ttgcctgggc cattgacatg
     2521 gctgatgaag actatgagtt ttagccagtc tcaagaggca gagttctgtg aagaggaaca
     2581 gtgtggtttg ggaaagatgg ataaactgag cctcacttgc cctcgtgcct gggggagaga
     2641 ggcagcaagt cttaacaaac caacatcttt gcgaaaagat aaacctggag atattataag
     2701 ggagagctga gccagttgtc ctatggacaa cttatttaaa aatatttcag atatcaaaat
     2761 tctagctgta tgatttgttt tgaattttgt ttttattttc aagagggcaa gtggatggga
     2821 atttgtcagc gttctaccag gcaaattcac tgtttcactg aaatgtttgg attctcttag
     2881 ctactgtatg caaagtccga ttatattggt gcgtttttac agttagggtt ttgcaataac
     2941 ttctatattt taatagaaat aaattcctaa actcccttcc ctctctccca tttcaggaat
     3001 ttaaaattaa gtagaacaaa aaacccagcg cacctgttag agtcgtcact ctctattgtc
     3061 atggggatca attttcatta aacttgaagc agtcgtggct ttggcagtgt tttggttcag
     3121 acacctgttc acagaaaaag catgatggga aaatatttcc tgacttgagt gttccttttt
     3181 aaatgtgaat ttttatttct ttttaattat tttaaaatat ttaaaccttt ttcttgatct
     3241 taaagatcgt gtagattggg gttggggagg gatgaagggc gagtgaatct aaggataatg
     3301 aaataatcag tgactgaaac cattttccca tcatcctttg ttctgagcat tcgctgtacc
     3361 ctttaagata tccatctttt tctttttaac cctaatcttt cacttgaaag attttattgt
     3421 ataaaaagtt tcacaggtca ataaacttag aggaaaatga gtatttggtc caaaaaaagg
     3481 aaaaataatc aagattttag ggcttttatt ttttcttttg taattgtgta aaaaatggaa
     3541 aaaaacataa aaagcagaat tttaatgtga agacattttt tgctataatc attagtttta
     3601 gaggcattgt tagtttagtg tgtgtgcaga gtccatttcc cacatctttc ctcaagtatc
     3661 ttctattttt atcatgaatt cccttttaat caactgtagg ttatttaaaa taaattccta
     3721 caacttaatg gaaa
//
