LOCUS       NM_001387777           10311 bp    mRNA    linear   PRI 03-OCT-2024
DEFINITION  Homo sapiens tensin 1 (TNS1), transcript variant 4, mRNA.
ACCESSION   NM_001387777 XM_024453083
VERSION     NM_001387777.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10311)
  AUTHORS   Zhou,H. and Wang,F.
  TITLE     Tensin 1 regulated by hepatic leukemia factor represses the
            progression of prostate cancer
  JOURNAL   Mutagenesis 38 (6), 295-304 (2023)
   PUBMED   37712764
  REMARK    GeneRIF: Tensin 1 regulated by hepatic leukemia factor represses
            the progression of prostate cancer.
REFERENCE   2  (bases 1 to 10311)
  AUTHORS   Wang,J., Tan,L., Yu,X., Cao,X., Jia,B., Chen,R. and Li,J.
  TITLE     lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation,
            migration, and angiogenesis via the miR-942/TNS1 axis and is
            positively regulated by the m6A reader YTHDC2
  JOURNAL   Mol Cancer 21 (1), 229 (2022)
   PUBMED   36581942
  REMARK    GeneRIF: lncRNA ZNRD1-AS1 promotes malignant lung cell
            proliferation, migration, and angiogenesis via the miR-942/TNS1
            axis and is positively regulated by the m[6]A reader YTHDC2.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10311)
  AUTHORS   Park,G.C., Kim,J.M., Shin,S.C., Cheon,Y.I., Sung,E.S., Lee,M.,
            Lee,J.C. and Lee,B.J.
  TITLE     Tensin Regulates Fundamental Biological Processes by Interacting
            with Integrins of Tonsil-Derived Mesenchymal Stem Cells
  JOURNAL   Cells 11 (15), 2333 (2022)
   PUBMED   35954177
  REMARK    GeneRIF: Tensin Regulates Fundamental Biological Processes by
            Interacting with Integrins of Tonsil-Derived Mesenchymal Stem
            Cells.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10311)
  AUTHORS   Zhu,C., Wang,S., Zheng,M., Chen,Z., Wang,G., Ma,J., Zhang,B.,
            Huang,W., Sun,X. and Wang,C.
  TITLE     miR-31-5p modulates cell progression in lung adenocarcinoma through
            TNS1/p53 axis
  JOURNAL   Strahlenther Onkol 198 (3), 304-314 (2022)
   PUBMED   35037949
  REMARK    GeneRIF: miR-31-5p modulates cell progression in lung
            adenocarcinoma through TNS1/p53 axis.
REFERENCE   5  (bases 1 to 10311)
  AUTHORS   Walton,N.I., Zhang,X., Soltis,A.R., Starr,J., Dalgard,C.L.,
            Wilkerson,M.D., Conrad,D. and Pollard,H.B.
  TITLE     Tensin 1 (TNS1) is a modifier gene for low body mass index (BMI) in
            homozygous [F508del]CFTR patients
  JOURNAL   Physiol Rep 9 (11), e14886 (2021)
   PUBMED   34086412
  REMARK    GeneRIF: Tensin 1 (TNS1) is a modifier gene for low body mass index
            (BMI) in homozygous [F508del]CFTR patients.
REFERENCE   6  (bases 1 to 10311)
  AUTHORS   Beaty,T.H., Hetmanski,J.B., Fallin,M.D., Park,J.W., Sull,J.W.,
            McIntosh,I., Liang,K.Y., Vanderkolk,C.A., Redett,R.J.,
            Boyadjiev,S.A., Jabs,E.W., Chong,S.S., Cheah,F.S., Wu-Chou,Y.H.,
            Chen,P.K., Chiu,Y.F., Yeow,V., Ng,I.S., Cheng,J., Huang,S., Ye,X.,
            Wang,H., Ingersoll,R. and Scott,A.F.
  TITLE     Analysis of candidate genes on chromosome 2 in oral cleft
            case-parent trios from three populations
  JOURNAL   Hum Genet 120 (4), 501-518 (2006)
   PUBMED   16953426
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 10311)
  AUTHORS   Yamashita,M., Horikoshi,S., Asanuma,K., Takahara,H., Shirato,I. and
            Tomino,Y.
  TITLE     Tensin is potentially involved in extracellular matrix production
            in mesangial cells
  JOURNAL   Histochem Cell Biol 121 (3), 245-254 (2004)
   PUBMED   14991332
  REMARK    GeneRIF: Tensin may play important roles in mesangial ECM
            production through an adhesion complex with integrin alpha5, FAK,
            and vinculin.
REFERENCE   8  (bases 1 to 10311)
  AUTHORS   Benzing,T., Gerke,P., Hopker,K., Hildebrandt,F., Kim,E. and Walz,G.
  TITLE     Nephrocystin interacts with Pyk2, p130(Cas), and tensin and
            triggers phosphorylation of Pyk2
  JOURNAL   Proc Natl Acad Sci U S A 98 (17), 9784-9789 (2001)
   PUBMED   11493697
REFERENCE   9  (bases 1 to 10311)
  AUTHORS   Chen,H., Ishii,A., Wong,W.K., Chen,L.B. and Lo,S.H.
  TITLE     Molecular characterization of human tensin
  JOURNAL   Biochem J 351 Pt 2 (Pt 2), 403-411 (2000)
   PUBMED   11023826
REFERENCE   10 (bases 1 to 10311)
  AUTHORS   Katz,B.Z., Zohar,M., Teramoto,H., Matsumoto,K., Gutkind,J.S.,
            Lin,D.C., Lin,S. and Yamada,K.M.
  TITLE     Tensin can induce JNK and p38 activation
  JOURNAL   Biochem Biophys Res Commun 272 (3), 717-720 (2000)
   PUBMED   10860821
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC010136.8 and AC009469.4.
            
            On Oct 8, 2020 this sequence version replaced XM_024453083.1.
            
            Summary: The protein encoded by this gene localizes to focal
            adhesions, regions of the plasma membrane where the cell attaches
            to the extracellular matrix. This protein crosslinks actin
            filaments and contains a Src homology 2 (SH2) domain, which is
            often found in molecules involved in signal transduction. This
            protein is a substrate of calpain II. Alternative splicing results
            in multiple transcript variants encoding different isoforms.
            [provided by RefSeq, Apr 2015].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.2924753.1,
                                           SRR14038195.1360091.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000682258.1/ ENSP00000506917.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-156               AC010136.8         54962-55117         c
            157-271             AC010136.8         43064-43178         c
            272-309             AC010136.8         30887-30924         c
            310-351             AC009469.4         140565-140606       c
            352-393             AC009469.4         127580-127621       c
            394-444             AC009469.4         126705-126755       c
            445-494             AC009469.4         120833-120882       c
            495-666             AC009469.4         118168-118339       c
            667-717             AC009469.4         115376-115426       c
            718-840             AC009469.4         113809-113931       c
            841-905             AC009469.4         113318-113382       c
            906-989             AC009469.4         111332-111415       c
            990-1102            AC009469.4         106904-107016       c
            1103-1163           AC009469.4         106414-106474       c
            1164-1239           AC009469.4         106114-106189       c
            1240-1369           AC009469.4         105405-105534       c
            1370-1435           AC009469.4         102716-102781       c
            1436-1552           AC009469.4         101268-101384       c
            1553-3130           AC009469.4         67880-69457         c
            3131-3327           AC009469.4         56385-56581         c
            3328-3403           AC009469.4         55461-55536         c
            3404-3496           AC009469.4         51825-51917         c
            3497-3695           AC009469.4         42110-42308         c
            3696-4765           AC009469.4         38060-39129         c
            4766-4852           AC009469.4         35282-35368         c
            4853-4984           AC009469.4         34055-34186         c
            4985-5077           AC009469.4         33585-33677         c
            5078-5155           AC009469.4         32738-32815         c
            5156-5227           AC009469.4         30618-30689         c
            5228-5396           AC009469.4         30193-30361         c
            5397-5465           AC009469.4         28973-29041         c
            5466-5498           AC009469.4         28445-28477         c
            5499-10311          AC009469.4         20161-24973         c
FEATURES             Location/Qualifiers
     source          1..10311
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q35"
     gene            1..10311
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /note="tensin 1"
                     /db_xref="GeneID:7145"
                     /db_xref="HGNC:HGNC:11973"
                     /db_xref="MIM:600076"
     exon            1..156
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..5643
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /note="isoform 4 is encoded by transcript variant 4;
                     matrix-remodelling associated 6;
                     Matrix-remodelling-associated protein 6; protein
                     phosphatase 1, regulatory subunit 155"
                     /codon_start=1
                     /product="tensin-1 isoform 4"
                     /protein_id="NP_001374706.1"
                     /db_xref="CCDS:CCDS92941.1"
                     /db_xref="GeneID:7145"
                     /db_xref="HGNC:HGNC:11973"
                     /db_xref="MIM:600076"
                     /translation="MTWICLSCMLWPEDLEAPKTHRFKVKTFKKVKPCGICRQVITQE
                     GCTCKVCSFSCHRKCQAKVAAPCVPPSNHELVPITTENAPKNVVDKGEGASRGGNTRK
                     SLEDNGSTRVTPSVQPHLQPIRNMSVSRTMEDSCELDLVYVTERIIAVSFPSTANEEN
                     FRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSI
                     CKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYE
                     DKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRI
                     YQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQ
                     FHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSV
                     DYNTSDPLIRWDSYDNFSGHRDDGMEEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVN
                     ATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELD
                     RLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETD
                     ILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGG
                     GYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSG
                     MFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLE
                     SLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKS
                     QSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPP
                     GRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPL
                     LSPKEATSDPSRTPEEEPLNLEGLVAHRVAGVQAREKQPAEPPAPLRRRAASDGQYEN
                     QSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSS
                     PPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGEPRSYVESVARTAVAGPRAQDS
                     EPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSD
                     QGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSP
                     ESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQ
                     MMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGR
                     HPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDR
                     RPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKR
                     RMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPE
                     TRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSP
                     PPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLAL
                     PCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKAT
                     SETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMK
                     TEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR"
     exon            157..271
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            272..309
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            310..351
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            352..393
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            394..444
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            445..494
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            495..666
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            667..717
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            718..840
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            841..905
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            906..989
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1102
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            1103..1163
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1239
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1369
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            1370..1435
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            1436..1552
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..3130
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            3131..3327
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            3328..3403
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            3404..3496
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            3497..3695
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            3696..4765
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            4766..4852
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            4853..4984
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            4985..5077
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            5078..5155
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            5156..5227
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            5228..5396
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            5397..5465
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            5466..5498
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     exon            5499..10311
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10292..10297
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /note="hexamer: GATAAA"
     polyA_site      10311
                     /gene="TNS1"
                     /gene_synonym="MST091; MST122; MST127; MSTP091; MSTP122;
                     MSTP127; MXRA6; PPP1R155; TNS"
                     /note="major polyA site"
ORIGIN      
        1 agatgctggc ggagccggag cagccgcgga ctcagagagc ccttctgcca gcgccgcggg
       61 gactctagct tcaggggctc ttcacaggcc ctgggcgtgc ctcagtgacg ggacccagca
      121 aagatgacgt ggatctgtct gtcctgcatg ctctggccag aggatctgga ggcccccaag
      181 acacaccgct tcaaggtgaa gaccttcaag aaggtgaagc cctgtgggat ctgccgccag
      241 gtcatcaccc aggaaggctg cacctgcaaa gtctgcagct tctcctgtca tcggaaatgc
      301 caggccaagg tggctgcccc ctgcgttcct ccatccaacc atgagctggt gcccatcacc
      361 actgagaatg caccaaagaa tgtagtggac aagggagaag gagcctcccg gggtggaaac
      421 acacggaaaa gcctcgagga caacggctcc accagggtca ccccgagtgt ccagccccac
      481 ctccagccca tcagaaacat gagtgtgagc cggaccatgg aggacagctg tgagctggac
      541 ctggtgtacg tcacagagag gatcatcgct gtctccttcc ccagcacagc caatgaggag
      601 aacttccgga gcaacctccg tgaggtggcg cagatgctca agtccaaaca tggaggcaac
      661 tacctgctgt tcaacctctc tgagcggaga cctgacatca cgaagctcca tgccaaggta
      721 ctggaatttg gctggcccga cctccacacc ccagccctgg agaagatctg cagcatctgt
      781 aaggccatgg acacatggct caatgcagac cctcacaatg tcgttgttct acacaacaag
      841 ggaaaccgag gcaggatagg agttgtcatc gcggcttaca tgcactacag caacatttct
      901 gccagtgcgg accaggctct ggaccggttt gcaatgaagc ggttctatga ggataagatt
      961 gtgcccattg gccagccatc ccaaagaagg tacgtgcatt acttcagtgg cctgctctcc
     1021 ggctccatca aaatgaacaa caagcccttg tttctgcacc acgtgatcat gcacggcatc
     1081 cccaactttg agtctaaagg aggatgtcgg ccatttctcc gcatctacca ggccatgcaa
     1141 cctgtgtaca catctggcat ctacaacatc ccaggagaca gccagactag cgtctgcatc
     1201 accatcgagc caggactgct cttgaaggga gacatcttgc tgaagtgcta ccacaagaag
     1261 ttccgaagcc cagcccgaga cgtcatcttc cgtgtgcagt tccacacctg tgccatccat
     1321 gacctggggg ttgtctttgg gaaggaggac cttgatgatg ctttcaaaga tgatcgattt
     1381 ccagagtatg gcaaagtgga gtttgtattt tcttatgggc cagagaaaat tcaaggcatg
     1441 gagcacctgg agaacgggcc gagcgtgtct gtggactata acacctccga ccccctcatc
     1501 cgctgggact cctacgacaa cttcagtggg catcgagatg acggcatgga ggaggtggtg
     1561 ggacacacgc aggggccact agatgggagc ctgtatgcta aggtgaagaa gaaagactcc
     1621 ctgcacggca gcaccggggc tgttaatgcc acacgtccta cactgtcggc cacccccaac
     1681 cacgtggaac acacgctttc tgtgagcagc gactcgggca actccacagc ctccaccaag
     1741 accgacaaga ccgacgagcc tgtccccggg gcctccagtg ccactgctgc cttgagtccc
     1801 caggagaagc gggagctgga ccgcctgctg agtggctttg gcttagagcg agagaagcaa
     1861 ggcgccatgt accacaccca gcacctcagg tcccgcccag cagggggctc ggctgtgccc
     1921 tcctctggac gccacgttgt cccagcccag gttcatgtca atggtggggc gttagcatct
     1981 gagcgggaga cagacatcct ggacgatgaa ttgccaaacc aggatggtca cagtgcgggc
     2041 agcatgggca cactctcttc tctggacggg gtcaccaaca ccagtgaggg gggctaccca
     2101 gaggccctgt ccccactgac caacggtctg gacaagtcct accccatgga gcctatggtc
     2161 aatggaggag gctaccccta cgagtctgcc agccgggcgg ggcctgccca tgctggccac
     2221 acggccccca tgcggccctc ctactctgca caggagggtt tagctggcta ccagagggag
     2281 gggccccacc cagcctggcc acagccagtg accacctccc actatgccca tgaccccagc
     2341 ggtatgttcc gctctcaatc cttttcggaa gctgaacccc agctgccccc agctccggtc
     2401 cgagggggaa gcagccggga ggctgtgcaa aggggactga attcgtggca gcagcagcag
     2461 cagcagcagc agcagcctcg cccacctcca cgccagcagg aaagagccca cttggagagt
     2521 cttgtagcca gcaggcccag ccctcagcca ttggcagaga cccccatccc cagtctccct
     2581 gagttcccgc gagcagcctc ccagcaggag attgaacagt ccatcgaaac actcaatatg
     2641 ctgatgctgg acctggagcc agcctccgct gctgccccac tacacaagtc ccagagtgtc
     2701 cccggggcct ggccaggggc ttctccactc tcctcccagc ccctctctgg atcctcccgt
     2761 cagtcccatc cactgaccca gtccagatct ggctatatcc ccagtgggca ttcgttggga
     2821 acccctgagc cagccccacg ggcctctctg gagtctgtcc ctcctggcag gtcttactca
     2881 ccttatgact atcagccatg tttggctggg cctaaccagg atttccattc aaagagccca
     2941 gcctcttcct ccttgcctgc cttccttccg accacccaca gccctccagg gcctcagcaa
     3001 cccccagcct ctctccctgg cctcactgct cagcctctgc tctcaccaaa ggaagcgact
     3061 tcagacccct cccggactcc agaggaggag ccattgaatt tagaagggct ggtggcccac
     3121 agggtagcag gggtacaggc tcgggagaag cagcctgcag agcccccagc ccctctgcgg
     3181 aggcgggcgg ccagtgatgg acagtatgag aaccagtctc cagaagccac atcccctcgt
     3241 agccctgggg ttcgctcccc tgtccagtgt gtctccccgg agctggctct taccatcgct
     3301 ctcaatcctg gagggcggcc caaagagccc catttgcaca gctacaagga ggccttcgag
     3361 gagatggagg gaacctcccc gagcagccca ccacccagtg gggtgcggtc ccccccgggt
     3421 ctggccaaga cacccctgtc tgctctgggc ctgaaacctc acaacccagc ggacatcctg
     3481 ttgcacccca caggagagcc ccggagctat gtggagtctg tggcacggac agcggtggct
     3541 ggaccccgag ctcaggactc tgagcccaag agctttagtg ctccagccac ccaggcctat
     3601 ggccatgaga tacccctgag gaacgggacc ctgggtggct cctttgtctc ccccagcccc
     3661 ctctccacca gcagccccat cctcagtgct gacagcactt cagtggggag tttcccgtcg
     3721 ggagagagca gtgaccaggg tccccggacg cccacccagc ctctgttgga gtctggcttc
     3781 cgctcaggca gcctgggaca gcccagcccg tctgcccaga gaaactacca gagctcttct
     3841 cctctcccga ctgtgggcag tagctacagc agccccgact actcacttca gcatttcagc
     3901 tcctctccgg aaagccaggc tcgagctcag ttcagtgtgg ctggcgtcca cacggtgcct
     3961 gggagccctc aggcgcgcca cagaacagtg ggcaccaaca ctccccctag tcctggcttc
     4021 ggctggcggg ccatcaatcc cagcatggct gcccccagca gtcccagttt gagccatcac
     4081 cagatgatgg gtccaccagg cactggcttc catggtagca ctgtctccag cccccagagc
     4141 agtgcagcga ccaccccggg gagccccagc ctgtgtcggc acccagcagg ggtctaccag
     4201 gtttctggcc tccacaacaa agtggccacc accccgggga gtcccagcct gggccggcac
     4261 cctggggctc accaaggcaa cctggcctcc ggtcttcata gcaatgcaat agccagccct
     4321 ggaagcccca gcctgggccg tcacctcgga gggtctggat ctgtggttcc cggcagcccc
     4381 tgcttggacc ggcatgtggc ctatggtggc tattctaccc cggaggatcg gagacccaca
     4441 ctgtcccggc agagcagtgc ctctggctac caggctcctt ccacgccctc cttccctgtc
     4501 tcccctgcct actaccctgg cctgagcagc cctgccacct ccccgtcacc agactccgca
     4561 gccttccggc aagggagccc aacaccagcc ttgccagaga agcgaaggat gtcagtggga
     4621 gaccgggcag gcagcctccc caactatgcc accatcaatg ggaaggtgtc ttcgcctgtc
     4681 gccagcggca tgtccagtcc cagtgggggc agcaccgtct ccttctccca cactctgccc
     4741 gacttctcca agtactccat gccagacaac agcccggaga cgcgggctaa agtgaagttt
     4801 gtccaggaca cttctaagta ttggtacaag cctgagatct ccagggagca ggccatcgcg
     4861 ctcctcaagg accaggagcc gggggccttc atcatccgcg acagtcactc cttccgaggc
     4921 gcgtacgggc tggccatgaa ggtgtcttcg ccacctccaa ccatcatgca gcagaataaa
     4981 aaaggagaca tgacccatga gctggtcagg cattttctga tagagactgg ccccagagga
     5041 gtcaagctca agggctgccc caatgagcca aacttcggat cgctgtctgc cctggtctac
     5101 cagcactcca tcatcccatt ggccctgcct tgcaagctgg tcattccaaa ccgagacccc
     5161 acagatgaat cgaaagatag ctccggccct gccaactcaa ctgcagacct gctgaaacaa
     5221 ggggcagcct gcaatgtgct cttcgtcaac tctgtggaca tggagtcact cactgggcca
     5281 caggccatct ctaaagccac atctgagacg ttggctgcag accccacgcc agctgccacc
     5341 atcgttcact tcaaagtctc tgcccaggga atcactctga ctgacaacca gagaaagctc
     5401 tttttcagac gccactaccc tctcaacact gtcaccttct gtgacctgga tccacaggaa
     5461 agaaagtgga tgaaaacaga gggtggtgcc cctgctaagc tcttcggctt cgtggcccgg
     5521 aagcagggca gcaccacgga caacgcctgc cacctctttg ctgagcttga ccccaaccag
     5581 ccggcctctg ccatcgtcaa cttcgtctcc aaggtcatgc tgaatgccgg ccaaaagaga
     5641 tgaaccctgc cccttgccca gggccagtgc catggggaag gggcttgtgg ggaggggacc
     5701 catgaatcct gaccactctt gaacccagaa ggaggacttt gggccaattt cggaggagag
     5761 aagaaagtgc aacgtgggga gagggaagtg aattgcagag gggaggggga aaagagagag
     5821 agagagagag agagagagag agagaaagat ggaggagaag aacttggatt cccctgggta
     5881 gatggaaact gcaaaaaccc aaagcctcca aaactaacca ggtccaccta acaccccctc
     5941 cctcccctaa gaagatggat gtcctcaaaa gagaaggaac aaacctcctt gggaatccac
     6001 attttttggg ggaatggaaa agctctgtct ccctaactca actgctttgc aaggggaaat
     6061 caagctggga gaatcttttt ctggccacct gtggggtagg ttgtcaaacc aaacagagcc
     6121 accgtgggac atcaagtgga agaacttgtt tgcttgaaag tatctcagac ccaaggcacc
     6181 tcaggtctct ttgctgtgcc tccactatat tgtcgtgtgg gtgtgtgtct gcacccacat
     6241 cctcacacat tgatctagat ctgcctttat ccactcgaat tataaacagc tcggcttgtc
     6301 cttgtcccat gtgtttgtag acacacatgc atactgtcca aagattaggg ttggtggtgg
     6361 cagtgcagca ggggagggac aaacaaccaa gctatgggtg acagaggctc tctcctggtg
     6421 cctgcacctg cactctagtg accctgggtg ccgccagacc cttctcttct acaaagaccc
     6481 cagcaggagt gggagggtct gcaatggcat cgccctgtcc tgccttggcc agaagcctgg
     6541 agctttggtt tgaggaggta gagatatgtg tatccatagg aagagatctg tcagaacagg
     6601 cagctgttga gctcggggtg tcttccccaa ggcatgtggc tcagcagcaa gaaaggcaag
     6661 ttgctcctgc tggggccctg gactctgcct tagctcccac ctctcagcct tgttattggg
     6721 tttcatgccc ctggaccagc cttatctcag acctgcttac ctgcatgatg cctttttggg
     6781 ggctggggat tgagtcttgc tgctctgccc agccctgttc tattctgcag ggtccctgtg
     6841 ttggaattct ccctggggaa cctactttct gctcagtgag gctccggcca gaaacctgga
     6901 gtccttatcc tcccctctgt aagtgtttta gggtctggct tttgcaggca ccctctgacc
     6961 tcagcagagc tcctgggcct gctgcctgca caccacatcg cctacctaca atgccaaagc
     7021 ctcactgtca ccctttctgc cttggtttcc ctagctgagc cacgctgccc atgcagcaga
     7081 gggcagaagg cttgcacttg ggccaaaggg cctaaggtcc actggacagt tgggaaaaca
     7141 cctgaccacc atttaaggac tctaagccag aatggaaaat tcaccaggac tccattctta
     7201 agcctatgcg agtcccctag agagaggcat tgtactgata tataaatatt atataatata
     7261 tacatgagac atactgacag aatctgtaag ctaataaaat gtaagaaaag gttaaaaaaa
     7321 gaataggtaa attgacaaga agtatttatt gtttttccat attgctttat tgccttcctt
     7381 ggggataaac caattcctat ccttttttat atgtgtaagt aaagcctgaa gtgtaggggg
     7441 cctttgttct tgaagcagcc agggtctcct tgccctggcc ttggccttcc ctagactgtg
     7501 tggggctcag cattgggagg ggttgcacat gtcccagcct ttggccccct tacttttcag
     7561 caagccaggg gcccagcagt cagctcccag gatgtgtggg gagctgtccc tgactctgca
     7621 ggcctgagcg agtgtgtgag catgcgggga catgggtgtg tatggcacac ataggtgcgt
     7681 gtgtgtcttt tgtatttttt ctcctccaag gagctgtgtc agtgtggacg ttctgtttca
     7741 gggagttgga aaggagggtg tctgcagaag gtggagagca ggggcagagg ccccactggc
     7801 caccccctgc ttcccagagt gaaaccttgt gcctggtgac caaagtccct ccaaagtgct
     7861 cttccttctg ggttattcaa gccaaatatc tgggtttccc cctctcctca ttccctagca
     7921 aaccccaatt atcttccaag ataggagata tttcccatcc ccttcctttg taaatatctc
     7981 atctcccact ggagagccca ggagcctatt cctggcatgg atgttctgtc cacacttgag
     8041 gctgggcggt gtatcagacc cttcaagcag cctggctggg gcccaggact gagtctgggg
     8101 tcagctttca cggtcgcttt tcccttcctc accacccacc acagcccacc ttgcatgcat
     8161 ggccagcccc tccactccag cctgagccat gtgtgcccct gcgggaggac ccattcatgc
     8221 cagaaagctg gtaactccct cccagcatcc ctgcggaagg agtcagtttc tgagagtgtg
     8281 acttttcaag gcgaatgatg gggaagggtt ccccagtccc cacagtggcc ccacctctgg
     8341 gccctgcacc agagcccttc tgtgtcacgg cgggctgtgc acccatgcac acacctacgc
     8401 acacacaaca ctccgcactg cagtatattc ttgccaaaga tttcctttaa aagcaagcac
     8461 ttttactaat tattattttg taaatgttta tcttcttctg tcttctccct ccctgaatct
     8521 attttactgt tgtttattgt tgaatctgtg tgtcagccag gagagcgctg tctggccttg
     8581 aacatgggct gggatgggaa agggtctggg agaagatggg caacaaagag ccagggagtc
     8641 atggacatcg cagcgacgca gaccccagca ggttcagtcc cgtgctgcca ccagctgtcc
     8701 agctgggtgt ctggagggaa gagggcagag gagggtcatg tcccttcagc tgggggaggg
     8761 gcccagtgag ctccacgtgg ctttttccca aagggagcaa gagggaagga ttgggcgaga
     8821 aaacaatgga gaggggacct gcgaaggaaa acagggagga agtgagcggt ttgatcagcc
     8881 tgctatcacg gtgttctggc tctcttattt agccaggcgc ttaagggaca gatacatcac
     8941 atcctaagtt tgggaaaggc ctttgaccca tgtcatctga gcgtctcctc cagtagctct
     9001 gaaagctgtg gacaccaatg gccaggattc cttctcccct ggtttttgag gatccctggg
     9061 tcttctgaga ctggccagga gagggatggt ggggccagtg gttgtgtgaa agcaggaggg
     9121 gcagccctcc tggacaagtg tgatccccct ataaacggct ctcaggaggt tagtgagtag
     9181 gagattctgc cttgttctga tgagcctgtg caggggctcc aggggagcat gctgtccagg
     9241 gggcacagaa gggtggtgag tgtgatcaaa tctagtctca ctcccacttt ttagtctcac
     9301 tcctactttt gtccaccacc cctgcctcct ggatcttctc ccactttttt tttcagcttt
     9361 aggacctggg gagatcctgt gagtcaaggc agacacccaa tcctgccccc acactcgggg
     9421 tcctccaaga ggttgggggg cagagtccca gagcagccct ttaccccagg tccaggccct
     9481 ggaatcctga gactcgcgtt tccttggcca gtggtaacac aggacgtgtg tgcgcatgtg
     9541 caagtgtgga tgtatgtgtg tgcgtgtgtt ttgctcattt ctttagggaa cttgggagtc
     9601 ggggttggag gtgctgggca atggaacttc aaattcaatg tcgcccagca gtgaggggag
     9661 tcgggaggtg aggcctgtag gccaaccaat tggtggagtc tcagcgatag cccaggtgag
     9721 aagtggttca cccagagggg cagggtgggg gcctcgggca gatctgtccc tcttggcacc
     9781 tctgtcctca aatgtccaaa atgttggagg acctctgttc atatcccacg cctgggctct
     9841 tgccagcagt ggagttactg tagagggatg tcccaagctt gttttccaat cagtgttaag
     9901 ctgtttgaaa ctctcctgtg tctgtgtttt gtttgtgcgt gtgtgtgaga gcacatcagt
     9961 gtgtgcaggc tgtgtttccc catttctctc ctcccttcag acccatcatt gagaacaaat
    10021 gtaagaaatc ccttcccacc accctccctg cctcccaggc cctctgcggg ggaaacaaga
    10081 tcacccagca tccttcccca ccccagctgt gtatttatat agatggaaat atactttata
    10141 ttttgtatca tcgtgcctat agccgctgcc accgtgtata aatcctggtg tatgctcctt
    10201 atcctggaca tgaatgtatt gtacactgac gcgtccccac tcctgtacag ctgctttgtt
    10261 tctttgcaat gcattgtatg gctttataaa tgataaagtt aaagaaaact c
//
